                         SEQUENCE LISTING

<110>  THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
       INOVIO PHARMACEUTICALS, INC.
       WEINER, David
       LANG, Krystle
       YAN, Jian
       DRAGHIA-AKLI, Ruxandra
       KHAN, Amir
 
<120>  IMPROVED HCV VACCINES AND METHODS FOR USING THE SAME

<130>  UPVG0024 WO2

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  876
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA Sequence of ConNS4B

<400>  1
gccaccatgg attggacctg gatcctgttc ctcgtggccg ctgccacaag agtgcacagc       60

agccagcatc tgccctacat cgaacagggc atgatgctgg ccgagcagtt caaacagaaa      120

gccctgggcc tgctgcagac agccagcaga cagggaagag gcattgcccc tgccgtgcag      180

accaactggc agaagctgga agccttctgg gccaagcaca tgtggaactt catcagcggc      240

atccagtacc tggccggcct gagcaccctg cctggcaacc ctgccattgc cagcctgatg      300

gccttcacag ccgccgtgac cagccctctg accacctccc agaccctgct gttcaacatc      360

ctgggcggat gggtggcagc ccagctggca gctcctggcg ccgctacagc ctttgtggga      420

gccggactgg ctggcgctgc catcggaagc gtgggcctgg gcaaggtgct ggtcgacatc      480

ctggccggct atggcgctgg cgtggctggg gccctggtgg cattcaagat catgagcggc      540

gaggtgccca gcaccgagga cctggtgaat ctgctgcccg ccattctgtc acctggcgct      600

ctggtggtgg gagtggtctg cgccgccatc ctgagaaggc atgtcggacc tggcgaaggt      660

gccgtgcagt ggatgaaccg gctgatcgcc ttcgccagca gaggcaacca cgtgtccccc      720

acccactacg tgcctgagag cgacgccgct gccagagtga cagccatcct gtccagcctg      780

accgtgaccc agctgctgcg gagactgcac cagtggatca gcagcgagtg caccaccccc      840

acctacccct acgacgtgcc cgactacgcc tgataa                                876


<210>  2
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein Sequence of ConNS4B

<400>  2

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Ser Gln His Leu Pro Tyr Ile Glu Gln Gly Met Met Leu Ala 
            20                  25                  30          


Glu Gln Phe Lys Gln Lys Ala Leu Gly Leu Leu Gln Thr Ala Ser Arg 
        35                  40                  45              


Gln Gly Arg Gly Ile Ala Pro Ala Val Gln Thr Asn Trp Gln Lys Leu 
    50                  55                  60                  


Glu Ala Phe Trp Ala Lys His Met Trp Asn Phe Ile Ser Gly Ile Gln 
65                  70                  75                  80  


Tyr Leu Ala Gly Leu Ser Thr Leu Pro Gly Asn Pro Ala Ile Ala Ser 
                85                  90                  95      


Leu Met Ala Phe Thr Ala Ala Val Thr Ser Pro Leu Thr Thr Ser Gln 
            100                 105                 110         


Thr Leu Leu Phe Asn Ile Leu Gly Gly Trp Val Ala Ala Gln Leu Ala 
        115                 120                 125             


Ala Pro Gly Ala Ala Thr Ala Phe Val Gly Ala Gly Leu Ala Gly Ala 
    130                 135                 140                 


Ala Ile Gly Ser Val Gly Leu Gly Lys Val Leu Val Asp Ile Leu Ala 
145                 150                 155                 160 


Gly Tyr Gly Ala Gly Val Ala Gly Ala Leu Val Ala Phe Lys Ile Met 
                165                 170                 175     


Ser Gly Glu Val Pro Ser Thr Glu Asp Leu Val Asn Leu Leu Pro Ala 
            180                 185                 190         


Ile Leu Ser Pro Gly Ala Leu Val Val Gly Val Val Cys Ala Ala Ile 
        195                 200                 205             


Leu Arg Arg His Val Gly Pro Gly Glu Gly Ala Val Gln Trp Met Asn 
    210                 215                 220                 


Arg Leu Ile Ala Phe Ala Ser Arg Gly Asn His Val Ser Pro Thr His 
225                 230                 235                 240 


Tyr Val Pro Glu Ser Asp Ala Ala Ala Arg Val Thr Ala Ile Leu Ser 
                245                 250                 255     


Ser Leu Thr Val Thr Gln Leu Leu Arg Arg Leu His Gln Trp Ile Ser 
            260                 265                 270         


Ser Glu Cys Thr Thr Pro Thr Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
        275                 280                 285             


<210>  3
<211>  1434
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA Sequence of ConNS5A

<400>  3
gccaccatgg attggacctg gatcctgttc ctggtggccg ctgctaccag agtgcacagc       60

agcggctctt ggctgagaga tgtgtgggac tggatctgca ccgtgctgac cgacttcaag      120

acctggctgc agagcaagct gctgcccaga ctgcccggcg tgcccttctt cagctgccag      180

cggggctaca agggcgtgtg gagaggcgac ggcatcatgc agaccacctg tcccggcgga      240

gcccagatca ccggccacgt gaagaacggc agcatgcgga tcgtgggccc caagacctgt      300

agcaacacct ggcacggcac cttccccatc aacgcctaca ccaccggccc ttgtaccccc      360

agccctgccc ccaattacag cagagccctg tggagagtgg ccgccgagga atacgtggaa      420

gtgaccagag tgggcgactt ccactacgtg accggcatga ccaccgacaa cgtgaagtgc      480

ccctgccagg tgccagcccc cgagttcttt accgaggtgg acggcgtgag actgcacaga      540

tacgcccctg cctgcaagcc cctgctgcgg gaggaagtga ccttccaagt cggcctgaac      600

cagtacctgg tgggaagcca gctgccctgc gagcctgaac ctgacgtggc cgtgctgaca      660

agcatgctga ccgatcccag ccacatcaca gccgaggccg ctggaagaag gctcgccaga      720

ggcagccctc ctagcctggc cagcagcagc gcctctcagc tgtccgcccc tagcctgaag      780

gccacctgta ccacccacca cgacagcccc gacgccgacc tgatcgaggc caatctgctg      840

tggcggcagg aaatgggcgg caacatcacc agagtggaga gcgagaacaa ggtggtgatc      900

ctggacagct tcgaccccct gagagccgaa gaggacgagc gggaagtgtc cgtgcccgcc      960

gagatcctgc ggaagtcccg gaagttcccc cctgccatgc ccatctgggc cagacctgac     1020

tacaaccccc ccctgctgga aagctggaag gaccccgact acgtgcctcc tgtggtgcac     1080

ggctgccctc tgcctccaac caaggcccct cccatccccc ctcccagacg gaagagaacc     1140

gtggtgctga cagagtccac cgtgtctagc gccctggccg agctggccac caagaccttc     1200

ggcagcagcg agagcagcgc cgtggattct ggcacagcca ccgcccctcc cgatcagcct     1260

agcgacgacg gcgacaccgg ctccgatgtg gagagctaca gcagcatgcc ccccctggaa     1320

ggcgaacccg gcgaccctga cctgagcgac ggcagctggt ctaccgtgtc cgaggaagcc     1380

agcgaggacg tcgtgtgctg ctacccctac gacgtgcccg actacgcctg ataa           1434


<210>  4
<211>  474
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein Sequence of ConNS5A

<400>  4

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Ser Gly Ser Trp Leu Arg Asp Val Trp Asp Trp Ile Cys Thr 
            20                  25                  30          


Val Leu Thr Asp Phe Lys Thr Trp Leu Gln Ser Lys Leu Leu Pro Arg 
        35                  40                  45              


Leu Pro Gly Val Pro Phe Phe Ser Cys Gln Arg Gly Tyr Lys Gly Val 
    50                  55                  60                  


Trp Arg Gly Asp Gly Ile Met Gln Thr Thr Cys Pro Gly Gly Ala Gln 
65                  70                  75                  80  


Ile Thr Gly His Val Lys Asn Gly Ser Met Arg Ile Val Gly Pro Lys 
                85                  90                  95      


Thr Cys Ser Asn Thr Trp His Gly Thr Phe Pro Ile Asn Ala Tyr Thr 
            100                 105                 110         


Thr Gly Pro Cys Thr Pro Ser Pro Ala Pro Asn Tyr Ser Arg Ala Leu 
        115                 120                 125             


Trp Arg Val Ala Ala Glu Glu Tyr Val Glu Val Thr Arg Val Gly Asp 
    130                 135                 140                 


Phe His Tyr Val Thr Gly Met Thr Thr Asp Asn Val Lys Cys Pro Cys 
145                 150                 155                 160 


Gln Val Pro Ala Pro Glu Phe Phe Thr Glu Val Asp Gly Val Arg Leu 
                165                 170                 175     


His Arg Tyr Ala Pro Ala Cys Lys Pro Leu Leu Arg Glu Glu Val Thr 
            180                 185                 190         


Phe Gln Val Gly Leu Asn Gln Tyr Leu Val Gly Ser Gln Leu Pro Cys 
        195                 200                 205             


Glu Pro Glu Pro Asp Val Ala Val Leu Thr Ser Met Leu Thr Asp Pro 
    210                 215                 220                 


Ser His Ile Thr Ala Glu Ala Ala Gly Arg Arg Leu Ala Arg Gly Ser 
225                 230                 235                 240 


Pro Pro Ser Leu Ala Ser Ser Ser Ala Ser Gln Leu Ser Ala Pro Ser 
                245                 250                 255     


Leu Lys Ala Thr Cys Thr Thr His His Asp Ser Pro Asp Ala Asp Leu 
            260                 265                 270         


Ile Glu Ala Asn Leu Leu Trp Arg Gln Glu Met Gly Gly Asn Ile Thr 
        275                 280                 285             


Arg Val Glu Ser Glu Asn Lys Val Val Ile Leu Asp Ser Phe Asp Pro 
    290                 295                 300                 


Leu Arg Ala Glu Glu Asp Glu Arg Glu Val Ser Val Pro Ala Glu Ile 
305                 310                 315                 320 


Leu Arg Lys Ser Arg Lys Phe Pro Pro Ala Met Pro Ile Trp Ala Arg 
                325                 330                 335     


Pro Asp Tyr Asn Pro Pro Leu Leu Glu Ser Trp Lys Asp Pro Asp Tyr 
            340                 345                 350         


Val Pro Pro Val Val His Gly Cys Pro Leu Pro Pro Thr Lys Ala Pro 
        355                 360                 365             


Pro Ile Pro Pro Pro Arg Arg Lys Arg Thr Val Val Leu Thr Glu Ser 
    370                 375                 380                 


Thr Val Ser Ser Ala Leu Ala Glu Leu Ala Thr Lys Thr Phe Gly Ser 
385                 390                 395                 400 


Ser Glu Ser Ser Ala Val Asp Ser Gly Thr Ala Thr Ala Pro Pro Asp 
                405                 410                 415     


Gln Pro Ser Asp Asp Gly Asp Thr Gly Ser Asp Val Glu Ser Tyr Ser 
            420                 425                 430         


Ser Met Pro Pro Leu Glu Gly Glu Pro Gly Asp Pro Asp Leu Ser Asp 
        435                 440                 445             


Gly Ser Trp Ser Thr Val Ser Glu Glu Ala Ser Glu Asp Val Val Cys 
    450                 455                 460                 


Cys Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
465                 470                 


<210>  5
<211>  1866
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA Sequence of ConNS5B

<400>  5
gccaccatgg attggacctg gatcctgttc ctggtggccg ctgccacaag agtgcacagc       60

agcatgagct actcttggac aggcgccctg gtgacacctt gtgccgccga ggaacagaag      120

ctccccatca acgccctgag caacagcctg ctgcggcacc acaacctggt gtacagcacc      180

acctccagaa gcgcctgtca gcggcagaaa aaagtgacct tcgaccggct gcaggtgctg      240

gacagccact accaggacgt gctgaaagaa gtgaaggctg ccgccagcaa agtgaaggcc      300

aatctgctgt ccgtggagga agcctgcagc ctgacacccc ctcacagcgc caagagcaag      360

ttcggctacg gcgccaagga tgtgcggtgc cacgccagaa aggccgtgaa ccacatcaac      420

agcgtgtgga aggatctgct ggaagatagc gtgaccccca tcgacaccac catcatggcc      480

aagaacgagg tgttctgcgt gcagcccgag aagggcggca gaaagcccgc cagactgatc      540

gtgttccccg acctgggcgt gagagtgtgc gagaagatgg ccctgtacga cgtggtgtcc      600

aagctgcctc tggccgtgat gggcagcagc tacggcttcc agtacagccc tggccagcgg      660

gtggaattcc tggtgcaggc ctggaagtcc aagaaaaccc ccatgggctt cagctacgac      720

accagatgct tcgacagcac tgtgaccgag agcgacatcc ggaccgagga agccatctac      780

cagtgctgcg acctggaccc tcaggccaga gtggccatca agagcctgac cgagagactg      840

tacgtgggcg gacctctgac caacagcaga ggcgagaact gcggcgccag aagatgtaga      900

gccagcggcg tgctgaccac ctcctgcggc aacaccctga cctgttacat caaggccaga      960

gccgcctgta gagccgccgg actgcaggac tgcaccatgc tggtgtgcgg cgacgacctg     1020

gtggtgatct gcgagtctgc cggcgtgcag gaagatgccg ccagcctgag agccttcacc     1080

gaggccatga ccagatacag cgcccctccc ggcgatcctc cccagcccga gtacgacctg     1140

gaactgatca ccagctgcag cagcaacgtg tccgtggccc acgatggcgc cggaaagcgg     1200

gtgtactacc tgaccaggga ccctaccaca cctctggcaa gggccgcttg ggagacagcc     1260

agacacaccc ccgtgaacag ctggctgggc aacatcatca tgttcgcccc caccctgtgg     1320

gcccggatga tcctgatgac ccacttcttc agcgtgctca tcgcccggga tcagctggaa     1380

caggccctgg actgcgagat ctacggcgcc tgctacagca tcgagcccct ggatctgccc     1440

cccatcatcc agagactgca cggcctgagc gccttctccc tgcacagcta cagcccaggc     1500

gagatcaaca gagtggccgc ctgcctgcgg aaactgggcg tgcctcctct gagagcctgg     1560

cggcacagag ccagatccgt gcgggccaga ctgctgtcaa gaggcggcag agcagccatc     1620

tgcggcaagt acctgttcaa ctgggccgtg cggaccaagc tgaagctgac ccctatcgcc     1680

gctgccggcc agctggatct gagcggctgg ttcacagccg gctacagcgg cggagacatc     1740

taccacagcg tgtcaagagc cagaccccgg tggttctggt tttgcctgct gctgctggcc     1800

gctggcgtgg gcatctatct gctgcccaac agatacccct acgacgtgcc cgactacgcc     1860

tgataa                                                                1866


<210>  6
<211>  618
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein Sequence of ConNS5B

<400>  6

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Ser Met Ser Tyr Ser Trp Thr Gly Ala Leu Val Thr Pro Cys 
            20                  25                  30          


Ala Ala Glu Glu Gln Lys Leu Pro Ile Asn Ala Leu Ser Asn Ser Leu 
        35                  40                  45              


Leu Arg His His Asn Leu Val Tyr Ser Thr Thr Ser Arg Ser Ala Cys 
    50                  55                  60                  


Gln Arg Gln Lys Lys Val Thr Phe Asp Arg Leu Gln Val Leu Asp Ser 
65                  70                  75                  80  


His Tyr Gln Asp Val Leu Lys Glu Val Lys Ala Ala Ala Ser Lys Val 
                85                  90                  95      


Lys Ala Asn Leu Leu Ser Val Glu Glu Ala Cys Ser Leu Thr Pro Pro 
            100                 105                 110         


His Ser Ala Lys Ser Lys Phe Gly Tyr Gly Ala Lys Asp Val Arg Cys 
        115                 120                 125             


His Ala Arg Lys Ala Val Asn His Ile Asn Ser Val Trp Lys Asp Leu 
    130                 135                 140                 


Leu Glu Asp Ser Val Thr Pro Ile Asp Thr Thr Ile Met Ala Lys Asn 
145                 150                 155                 160 


Glu Val Phe Cys Val Gln Pro Glu Lys Gly Gly Arg Lys Pro Ala Arg 
                165                 170                 175     


Leu Ile Val Phe Pro Asp Leu Gly Val Arg Val Cys Glu Lys Met Ala 
            180                 185                 190         


Leu Tyr Asp Val Val Ser Lys Leu Pro Leu Ala Val Met Gly Ser Ser 
        195                 200                 205             


Tyr Gly Phe Gln Tyr Ser Pro Gly Gln Arg Val Glu Phe Leu Val Gln 
    210                 215                 220                 


Ala Trp Lys Ser Lys Lys Thr Pro Met Gly Phe Ser Tyr Asp Thr Arg 
225                 230                 235                 240 


Cys Phe Asp Ser Thr Val Thr Glu Ser Asp Ile Arg Thr Glu Glu Ala 
                245                 250                 255     


Ile Tyr Gln Cys Cys Asp Leu Asp Pro Gln Ala Arg Val Ala Ile Lys 
            260                 265                 270         


Ser Leu Thr Glu Arg Leu Tyr Val Gly Gly Pro Leu Thr Asn Ser Arg 
        275                 280                 285             


Gly Glu Asn Cys Gly Ala Arg Arg Cys Arg Ala Ser Gly Val Leu Thr 
    290                 295                 300                 


Thr Ser Cys Gly Asn Thr Leu Thr Cys Tyr Ile Lys Ala Arg Ala Ala 
305                 310                 315                 320 


Cys Arg Ala Ala Gly Leu Gln Asp Cys Thr Met Leu Val Cys Gly Asp 
                325                 330                 335     


Asp Leu Val Val Ile Cys Glu Ser Ala Gly Val Gln Glu Asp Ala Ala 
            340                 345                 350         


Ser Leu Arg Ala Phe Thr Glu Ala Met Thr Arg Tyr Ser Ala Pro Pro 
        355                 360                 365             


Gly Asp Pro Pro Gln Pro Glu Tyr Asp Leu Glu Leu Ile Thr Ser Cys 
    370                 375                 380                 


Ser Ser Asn Val Ser Val Ala His Asp Gly Ala Gly Lys Arg Val Tyr 
385                 390                 395                 400 


Tyr Leu Thr Arg Asp Pro Thr Thr Pro Leu Ala Arg Ala Ala Trp Glu 
                405                 410                 415     


Thr Ala Arg His Thr Pro Val Asn Ser Trp Leu Gly Asn Ile Ile Met 
            420                 425                 430         


Phe Ala Pro Thr Leu Trp Ala Arg Met Ile Leu Met Thr His Phe Phe 
        435                 440                 445             


Ser Val Leu Ile Ala Arg Asp Gln Leu Glu Gln Ala Leu Asp Cys Glu 
    450                 455                 460                 


Ile Tyr Gly Ala Cys Tyr Ser Ile Glu Pro Leu Asp Leu Pro Pro Ile 
465                 470                 475                 480 


Ile Gln Arg Leu His Gly Leu Ser Ala Phe Ser Leu His Ser Tyr Ser 
                485                 490                 495     


Pro Gly Glu Ile Asn Arg Val Ala Ala Cys Leu Arg Lys Leu Gly Val 
            500                 505                 510         


Pro Pro Leu Arg Ala Trp Arg His Arg Ala Arg Ser Val Arg Ala Arg 
        515                 520                 525             


Leu Leu Ser Arg Gly Gly Arg Ala Ala Ile Cys Gly Lys Tyr Leu Phe 
    530                 535                 540                 


Asn Trp Ala Val Arg Thr Lys Leu Lys Leu Thr Pro Ile Ala Ala Ala 
545                 550                 555                 560 


Gly Gln Leu Asp Leu Ser Gly Trp Phe Thr Ala Gly Tyr Ser Gly Gly 
                565                 570                 575     


Asp Ile Tyr His Ser Val Ser Arg Ala Arg Pro Arg Trp Phe Trp Phe 
            580                 585                 590         


Cys Leu Leu Leu Leu Ala Ala Gly Val Gly Ile Tyr Leu Leu Pro Asn 
        595                 600                 605             


Arg Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
    610                 615             


<210>  7
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IgE Leader DNA sequence for NS5A

<400>  7
atggattgga cctggatcct gttcctggtg gccgctgcta ccagagtgca cagc             54


<210>  8
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IgE Leader DNA sequence for NS5B and NS4B

<400>  8
atggattgga cctggatcct gttcctggtg gccgctgcca caagagtgca cagc             54


<210>  9
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgE leader protein

<400>  9

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser 
        


