                         SEQUENCE LISTING

<110>  Keygene N.V.
 
<120>  Methods and Compositions for Producing Drimenol

<130>  P30952PC00/RKI

<150>  US61/548,934
<151>  2011-10-19

<150>  NL 2007626
<151>  2011-10-20

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  1672
<212>  DNA
<213>  Valeriana officinalis

<400>  1
atgtctactg cattaaacag tgagcatgaa actgttcgtc cattagcaag ttttcaacca       60

agtacatggg gcgatctttt catctcttat tctgaagata gccagcttaa ggaagtatat      120

ggtaatgagc acgaatgtct gaaacaacaa gtgaaaacaa tgttgttgga tgtgacaaat      180

tatagaattt ccgagaaaat cgctttcata aatacgttgg agagattagg ggtatctcat      240

gagtttgaga atgagattga aggtctgctt catcaaatgt ttgatgctca ttctaaattc      300

caagatggta ttcaacactt tgatttgttc acattgggga tttacttcag gattctcagg      360

caacatggct atagaatcta ttgtgatgtt ttcaacaagt tgaaagatag caacaatgaa      420

ttcaagaagg aacttaaaga ggacgcgatc ggtttgctaa gtttgtacga agcaacacaa      480

gtaagagcac acgctgaaga aattttagac gaagccctca ttttcacaaa ggctcaactt      540

gaatccatag ccgcaacctc cagcttaagc ccatttgtcg agaagcaaat tactcatgct      600

ttggtccaag ctctccacaa aggaatccca agagtcgaat cgcgccattt catctctgtt      660

tatgaagaag atcctgacaa aaatgatttg ttgttgaggt tctcaaagat tgattacaat      720

attgtacaaa tgcttcacaa gcaagaattg tgccatatct caaagtggtg gagagattcg      780

gagctcgaaa caaaactaac ttatgcgagg aatagagtgg cggaatgctt tttatggact      840

ctttgtgtgt accacgaacc aaagtactct ccggctcggc ttctgttagg caaactcata      900

aatatcatat cttgcactga tgacacatat gatgcgtatg gtacattaga ggaagttcag      960

atctttacag atgtcataca aaggttggat aggagttcta tggagcagct gccggattac     1020

atgaaaatcc tctacaaagc tgtccttgat cttttcgacg aagtagaagt tcagctatcg     1080

aaccaagaaa ctaataatac ttatcgtatg gcttatgcca aggaagagtt aaaagctatc     1140

gccaagtgct acgaaaagga gcacatatgg ttcagaaaat gtcacgtgcc cccattcgaa     1200

gaatatctag agaatgcggt agtgtcaatc ggtaatcgtt tggccgtaac tttttctttt     1260

ctgggaatgg atcaagtagc agctgttgaa gcgttcgagt gggccaaaac tgatcccaaa     1320

atggtaaaat cgtgcggtaa agtcttacga cttgttgacg acgtaatgag ccacgaggag     1380

gaagatgtaa gaggacacgt ggcaacggga gtcgaatgct acatgaaaga acacggagtg     1440

agtagggaag aggccgtcgt ggagttctac aagagggtcg agtacgcgtg gaaggatgtg     1500

aacgaggaat ttataacgcc gaaccatctg catatcgacc tcctcaaccg cgttcttaac     1560

cttacaagaa ttgcagacgt tgtttacaag tttgaagacg gctacacgca ccccgagaag     1620

actctgaaac atcatatcat ggcgttgttc gtcgaccccg tccccgtata ga             1672


<210>  2
<211>  555
<212>  PRT
<213>  Valeriana officinalis

<400>  2

Met Ser Thr Ala Leu Asn Ser Glu His Glu Thr Val Arg Pro Leu Ala 
1               5                   10                  15      


Ser Phe Gln Pro Ser Thr Trp Gly Asp Leu Phe Ile Ser Tyr Ser Glu 
            20                  25                  30          


Asp Ser Gln Leu Lys Glu Val Tyr Gly Asn Glu His Glu Cys Leu Lys 
        35                  40                  45              


Gln Gln Val Lys Thr Met Leu Leu Asp Val Thr Asn Tyr Arg Ile Ser 
    50                  55                  60                  


Glu Lys Ile Ala Phe Ile Asn Thr Leu Glu Arg Leu Gly Val Ser His 
65                  70                  75                  80  


Glu Phe Glu Asn Glu Ile Glu Gly Leu Leu His Gln Met Phe Asp Ala 
                85                  90                  95      


His Ser Lys Phe Gln Asp Gly Ile Gln His Phe Asp Leu Phe Thr Leu 
            100                 105                 110         


Gly Ile Tyr Phe Arg Ile Leu Arg Gln His Gly Tyr Arg Ile Tyr Cys 
        115                 120                 125             


Asp Val Phe Asn Lys Leu Lys Asp Ser Asn Asn Glu Phe Lys Lys Glu 
    130                 135                 140                 


Leu Lys Glu Asp Ala Ile Gly Leu Leu Ser Leu Tyr Glu Ala Thr Gln 
145                 150                 155                 160 


Val Arg Ala His Ala Glu Glu Ile Leu Asp Glu Ala Leu Ile Phe Thr 
                165                 170                 175     


Lys Ala Gln Leu Glu Ser Ile Ala Ala Thr Ser Ser Leu Ser Pro Phe 
            180                 185                 190         


Val Glu Lys Gln Ile Thr His Ala Leu Val Gln Ala Leu His Lys Gly 
        195                 200                 205             


Ile Pro Arg Val Glu Ser Arg His Phe Ile Ser Val Tyr Glu Glu Asp 
    210                 215                 220                 


Pro Asp Lys Asn Asp Leu Leu Leu Arg Phe Ser Lys Ile Asp Tyr Asn 
225                 230                 235                 240 


Ile Val Gln Met Leu His Lys Gln Glu Leu Cys His Ile Ser Lys Trp 
                245                 250                 255     


Trp Arg Asp Ser Glu Leu Glu Thr Lys Leu Thr Tyr Ala Arg Asn Arg 
            260                 265                 270         


Val Ala Glu Cys Phe Leu Trp Thr Leu Cys Val Tyr His Glu Pro Lys 
        275                 280                 285             


Tyr Ser Pro Ala Arg Leu Leu Leu Gly Lys Leu Ile Asn Ile Ile Ser 
    290                 295                 300                 


Cys Thr Asp Asp Thr Tyr Asp Ala Tyr Gly Thr Leu Glu Glu Val Gln 
305                 310                 315                 320 


Ile Phe Thr Asp Val Ile Gln Arg Leu Asp Arg Ser Ser Met Glu Gln 
                325                 330                 335     


Leu Pro Asp Tyr Met Lys Ile Leu Tyr Lys Ala Val Leu Asp Leu Phe 
            340                 345                 350         


Asp Glu Val Glu Val Gln Leu Ser Asn Gln Glu Thr Asn Asn Thr Tyr 
        355                 360                 365             


Arg Met Ala Tyr Ala Lys Glu Glu Leu Lys Ala Ile Ala Lys Cys Tyr 
    370                 375                 380                 


Glu Lys Glu His Ile Trp Phe Arg Lys Cys His Val Pro Pro Phe Glu 
385                 390                 395                 400 


Glu Tyr Leu Glu Asn Ala Val Val Ser Ile Gly Asn Arg Leu Ala Val 
                405                 410                 415     


Thr Phe Ser Phe Leu Gly Met Asp Gln Val Ala Ala Val Glu Ala Phe 
            420                 425                 430         


Glu Trp Ala Lys Thr Asp Pro Lys Met Val Lys Ser Cys Gly Lys Val 
        435                 440                 445             


Leu Arg Leu Val Asp Asp Val Met Ser His Glu Glu Glu Asp Val Arg 
    450                 455                 460                 


Gly His Val Ala Thr Gly Val Glu Cys Tyr Met Lys Glu His Gly Val 
465                 470                 475                 480 


Ser Arg Glu Glu Ala Val Val Glu Phe Tyr Lys Arg Val Glu Tyr Ala 
                485                 490                 495     


Trp Lys Asp Val Asn Glu Glu Phe Ile Thr Pro Asn His Leu His Asp 
            500                 505                 510         


Leu Leu Asn Arg Val Leu Asn Leu Thr Arg Ile Ala Asp Val Val Tyr 
        515                 520                 525             


Lys Phe Glu Asp Gly Tyr Thr His Pro Glu Lys Thr Leu Lys His His 
    530                 535                 540                 


Ile Met Ala Leu Phe Val Asp Pro Val Pro Val 
545                 550                 555 


<210>  3
<211>  1680
<212>  DNA
<213>  Persicaria hydropiper

<400>  3
atgtctactg ccgttaacgt cccatctgcg gtccgccccg ccgacaagcg tccgattgcg       60

agctttcacc cgagcccatg gggcgactac ttcctcaaat acgttccttg tgaccaggtg      120

actcaagcca agatggaaga tgaggtgaag aaagttgaag aggatgtaaa gaaggagttg      180

cggaagctgg cgaaggctgt agggaagcca ttggagctgc tcaacttcat cgatgtcgtc      240

gaacgccttg gggtgggata ccgccttgag caggagatcg aggaccttgt tcaagctata      300

ttcgacaacg acaaatttgg agtcgatgaa ttcgatctct atcatacttc cctctggttt      360

cgcctcctta ggcaacatgg gtttcacgta tcatgtgatg tgttcggaaa attcaagggc      420

agaaacggaa ggttcaagga ctcgttggcg agtgatgtga aggggatact cggcttgtac      480

gaagcctcac atgttcgcac ccatggcgat gacacgcttg atgaagcatt ggtgtttact      540

acgactcatc ttaaagccgt agtgaccaac caaccaaacc atcccttggt gccacaagtg      600

acccatgccc taatgcagcc ctaccacaag ggcatgccaa ggctcgagtc taggcacttc      660

atcgcattct acgagaaaga tccttaccac gacaaaacct tgttgaaatt tggcaaattg      720

gacttcaact tggtgcaagc attgcacaag aaggagctca aagatctcag caggtggtgg      780

aaagatctag atatgcacgc gaagatgcct ttcccgagca gagaccgagt gcccgaaggc      840

tacttttgga cactagggcc tttctatgaa ccacaattcg ctctttgtcg aaaatttttc      900

ttgcaagtgt tcaaagtaac ttccattgtc gatgatatct acgatgccta tggaactatc      960

gatgagctca ccgctttcac taaagctgct gagagatggg atcgtagttg ccttgatgaa     1020

cttccggaat acatgaaagt gagctacgcg tctctcattg ataccttcga ggaatttgaa     1080

cgcgacttgg ctccccaagg aagatcttgg agcgtcaagt acgcaagaga ggaaatgata     1140

cagatgtgta gagtttacta ccaagaagcg aaatggtgcc atgagaaata ctcgcccacc     1200

tgcgacgagt acttggagaa agcatccata gtgagtttcg gctacaactt gggaacagta     1260

gtgtgcttcc tcgggatggg agacgtcgct acaaaggagg cattcgaatg ggctcgcgga     1320

aacccaaagg tcgtaagagc cgcaggcata atcggaaggc tcatggacga cataggcagc     1380

catcattttg agcaaggtag agaccatgtt ccatccgccg tggagtgcta cataaggcag     1440

cacggtgtcg acgaagtaac cgcccaaaga gagttgggaa agcgggtgga aagtagctgg     1500

aaggacatca atgagatgat gttgaagcct tatatgatgc cgaagcctct tctaactcgc     1560

atccttaacg agtgtcgcat tgtggatgtg atctacaagg gagaagatag ctacaccttc     1620

tccaacacca ccatgaagaa aaacatttct cacattctca ccgatcccat tccgatttag     1680


<210>  4
<211>  558
<212>  PRT
<213>  P. hydropiper

<400>  4

Met Ser Thr Ala Val Asn Val Pro Ser Ala Val Arg Pro Ala Asp Lys 
1               5                   10                  15      


Arg Pro Ile Ala Ser Phe His Pro Ser Pro Trp Gly Asp Tyr Phe Leu 
            20                  25                  30          


Lys Tyr Val Pro Cys Asp Gln Val Thr Gln Ala Lys Met Glu Asp Glu 
        35                  40                  45              


Val Lys Lys Val Glu Glu Asp Val Lys Lys Glu Leu Arg Lys Leu Ala 
    50                  55                  60                  


Lys Ala Val Gly Lys Pro Leu Glu Leu Leu Asn Phe Ile Asp Val Val 
65                  70                  75                  80  


Glu Arg Leu Gly Val Gly Tyr Arg Leu Glu Gln Glu Ile Glu Asp Leu 
                85                  90                  95      


Val Gln Ala Ile Phe Asp Asn Asp Lys Phe Gly Val Asp Glu Phe Asp 
            100                 105                 110         


Leu Tyr His Thr Ser Leu Trp Phe Arg Leu Leu Arg Gln His Gly Phe 
        115                 120                 125             


His Val Ser Cys Asp Val Phe Gly Lys Phe Lys Gly Arg Asn Gly Arg 
    130                 135                 140                 


Phe Lys Asp Ser Leu Ala Ser Asp Val Lys Gly Ile Leu Gly Leu Tyr 
145                 150                 155                 160 


Glu Ala Ser His Val Arg Thr His Gly Asp Asp Thr Leu Asp Glu Ala 
                165                 170                 175     


Leu Val Phe Thr Thr Thr His Leu Lys Ala Val Val Thr Asn Gln Pro 
            180                 185                 190         


Asn His Pro Leu Val Pro Gln Val Thr His Ala Leu Met Gln Pro Tyr 
        195                 200                 205             


His Lys Gly Met Pro Arg Leu Glu Ser Arg His Phe Ile Ala Phe Tyr 
    210                 215                 220                 


Glu Lys Asp Pro Tyr His Asp Lys Thr Leu Leu Lys Phe Gly Lys Leu 
225                 230                 235                 240 


Asp Phe Asn Leu Val Gln Ala Leu His Lys Lys Glu Leu Lys Asp Leu 
                245                 250                 255     


Ser Arg Trp Trp Lys Asp Leu Asp Met His Ala Lys Met Pro Phe Pro 
            260                 265                 270         


Ser Arg Asp Arg Val Pro Glu Gly Phe Trp Thr Leu Gly Pro Phe Tyr 
        275                 280                 285             


Glu Pro Gln Phe Ala Leu Cys Arg Lys Phe Phe Leu Gln Val Phe Lys 
    290                 295                 300                 


Val Thr Ser Ile Val Asp Asp Ile Tyr Asp Ala Tyr Gly Thr Ile Asp 
305                 310                 315                 320 


Glu Leu Thr Ala Phe Thr Lys Ala Ala Glu Arg Trp Asp Arg Ser Cys 
                325                 330                 335     


Leu Asp Glu Leu Pro Glu Tyr Met Lys Val Ser Tyr Ala Ser Leu Ile 
            340                 345                 350         


Asp Thr Phe Glu Glu Phe Glu Arg Asp Leu Ala Pro Gln Gly Arg Ser 
        355                 360                 365             


Trp Ser Val Lys Tyr Ala Arg Glu Glu Met Ile Gln Met Cys Arg Val 
    370                 375                 380                 


Tyr Tyr Gln Glu Ala Lys Trp Cys His Glu Lys Tyr Ser Pro Thr Cys 
385                 390                 395                 400 


Asp Glu Tyr Leu Glu Lys Ala Ser Ile Val Ser Phe Gly Tyr Asn Leu 
                405                 410                 415     


Gly Thr Val Val Cys Phe Leu Gly Met Gly Asp Val Ala Thr Lys Glu 
            420                 425                 430         


Ala Phe Glu Trp Ala Arg Gly Asn Pro Lys Val Val Arg Ala Ala Gly 
        435                 440                 445             


Ile Ile Gly Arg Leu Met Asp Asp Ile Gly Ser His His Phe Glu Gln 
    450                 455                 460                 


Gly Arg Asp His Val Pro Ser Ala Val Glu Cys Tyr Ile Arg Gln His 
465                 470                 475                 480 


Gly Val Asp Glu Val Thr Ala Gln Arg Glu Leu Gly Lys Arg Val Glu 
                485                 490                 495     


Ser Ser Trp Lys Asp Ile Asn Glu Met Met Leu Lys Pro Tyr Met Met 
            500                 505                 510         


Pro Lys Pro Leu Leu Thr Arg Ile Leu Asn Glu Cys Arg Ile Val Asp 
        515                 520                 525             


Val Ile Tyr Lys Gly Glu Asp Ser Tyr Thr Phe Ser Asn Thr Thr Met 
    530                 535                 540                 


Lys Lys Asn Ile Ser His Ile Leu Thr Asp Pro Ile Pro Ile 
545                 550                 555             


<210>  5
<211>  437
<212>  PRT
<213>  Arabidopsis thaliana

<400>  5

Met Asp Pro Thr Glu Ser Leu Pro Glu Glu Asp Glu Glu Ile Val Lys 
1               5                   10                  15      


Ser Val Ile Asp Gly Val Ile Pro Ser Tyr Ser Leu Glu Ser Arg Leu 
            20                  25                  30          


Gly Asp Cys Lys Arg Ala Ala Ser Ile Arg Arg Glu Ala Leu Gln Arg 
        35                  40                  45              


Val Thr Gly Arg Ser Ile Glu Gly Leu Pro Leu Asp Gly Phe Asp Tyr 
    50                  55                  60                  


Glu Ser Ile Leu Gly Gln Cys Cys Glu Met Pro Val Gly Tyr Ile Gln 
65                  70                  75                  80  


Ile Pro Val Gly Ile Ala Gly Pro Leu Leu Leu Asp Gly Tyr Glu Tyr 
                85                  90                  95      


Ser Val Pro Met Ala Thr Thr Glu Gly Cys Leu Val Ala Ser Thr Asn 
            100                 105                 110         


Arg Gly Cys Lys Ala Met Phe Ile Ser Gly Gly Ala Thr Ser Thr Val 
        115                 120                 125             


Leu Lys Asp Gly Met Thr Arg Ala Pro Val Val Arg Phe Ala Ser Ala 
    130                 135                 140                 


Arg Arg Ala Ser Glu Leu Lys Phe Phe Leu Glu Asn Pro Glu Asn Phe 
145                 150                 155                 160 


Asp Thr Leu Ala Val Val Phe Asn Arg Ser Ser Arg Phe Ala Arg Leu 
                165                 170                 175     


Gln Ser Val Lys Cys Thr Ile Ala Gly Lys Asn Ala Tyr Val Arg Phe 
            180                 185                 190         


Cys Cys Ser Thr Gly Asp Ala Met Gly Met Asn Met Val Ser Lys Gly 
        195                 200                 205             


Val Gln Asn Val Leu Glu Tyr Leu Thr Asp Asp Phe Pro Asp Met Asp 
    210                 215                 220                 


Val Ile Gly Ile Ser Gly Asn Phe Cys Ser Asp Lys Lys Pro Ala Ala 
225                 230                 235                 240 


Val Asn Trp Ile Glu Gly Arg Gly Lys Ser Val Val Cys Glu Ala Val 
                245                 250                 255     


Ile Arg Gly Glu Ile Val Asn Lys Val Leu Lys Thr Ser Val Ala Ala 
            260                 265                 270         


Leu Val Glu Leu Asn Met Leu Lys Asn Leu Ala Gly Ser Ala Val Ala 
        275                 280                 285             


Gly Ser Leu Gly Gly Phe Asn Ala His Ala Ser Asn Ile Val Ser Ala 
    290                 295                 300                 


Val Phe Ile Ala Thr Gly Gln Asp Pro Ala Gln Asn Val Glu Ser Ser 
305                 310                 315                 320 


Gln Cys Ile Thr Met Met Glu Ala Ile Asn Asp Gly Lys Asp Ile His 
                325                 330                 335     


Ile Ser Val Thr Met Pro Ser Ile Glu Val Gly Thr Val Gly Gly Gly 
            340                 345                 350         


Thr Gln Leu Ala Ser Gln Ser Ala Cys Leu Asn Leu Leu Gly Val Lys 
        355                 360                 365             


Gly Ala Ser Thr Glu Ser Pro Gly Met Asn Ala Arg Arg Leu Ala Thr 
    370                 375                 380                 


Ile Val Ala Gly Ala Val Leu Ala Gly Glu Leu Ser Leu Met Ser Ala 
385                 390                 395                 400 


Ile Ala Ala Gly Gln Leu Val Arg Ser His Met Lys Tyr Asn Arg Ser 
                405                 410                 415     


Ser Arg Asp Ile Ser Gly Ala Thr Thr Thr Thr Thr Thr Thr Thr Ala 
            420                 425                 430         


Ala Ala Asp Leu Gln 
        435         


<210>  6
<211>  342
<212>  PRT
<213>  Arabidopsis thaliana

<400>  6

Met Ala Asp Leu Lys Ser Thr Phe Leu Asp Val Tyr Ser Val Leu Lys 
1               5                   10                  15      


Ser Asp Leu Leu Gln Asp Pro Ser Phe Glu Phe Thr His Glu Ser Arg 
            20                  25                  30          


Gln Trp Leu Glu Arg Met Leu Asp Tyr Asn Val Arg Gly Gly Lys Leu 
        35                  40                  45              


Asn Arg Gly Leu Ser Val Val Asp Ser Tyr Lys Leu Leu Lys Gln Gly 
    50                  55                  60                  


Gln Asp Leu Thr Glu Lys Glu Thr Phe Leu Ser Cys Ala Leu Gly Trp 
65                  70                  75                  80  


Cys Ile Glu Trp Leu Gln Ala Tyr Phe Leu Val Leu Asp Asp Ile Met 
                85                  90                  95      


Asp Asn Ser Val Thr Arg Arg Gly Gln Pro Cys Trp Phe Arg Lys Pro 
            100                 105                 110         


Lys Val Gly Met Ile Ala Ile Asn Asp Gly Ile Leu Leu Arg Asn His 
        115                 120                 125             


Ile His Arg Ile Leu Lys Lys His Phe Arg Glu Met Pro Tyr Tyr Val 
    130                 135                 140                 


Asp Leu Val Asp Leu Phe Asn Glu Val Glu Phe Gln Thr Ala Cys Gly 
145                 150                 155                 160 


Gln Met Ile Asp Leu Ile Thr Thr Phe Asp Gly Glu Lys Asp Leu Ser 
                165                 170                 175     


Lys Tyr Ser Leu Gln Ile His Arg Arg Ile Val Glu Tyr Lys Thr Ala 
            180                 185                 190         


Tyr Tyr Ser Phe Tyr Leu Pro Val Ala Cys Ala Leu Leu Met Ala Gly 
        195                 200                 205             


Glu Asn Leu Glu Asn His Thr Asp Val Lys Thr Val Leu Val Asp Met 
    210                 215                 220                 


Gly Ile Tyr Phe Gln Val Gln Asp Asp Tyr Leu Asp Cys Phe Ala Asp 
225                 230                 235                 240 


Pro Glu Thr Leu Gly Lys Ile Gly Thr Asp Ile Glu Asp Phe Lys Cys 
                245                 250                 255     


Ser Trp Leu Val Val Lys Ala Leu Glu Arg Cys Ser Glu Glu Gln Thr 
            260                 265                 270         


Lys Ile Leu Tyr Glu Asn Tyr Gly Lys Ala Glu Pro Ser Asn Val Ala 
        275                 280                 285             


Lys Val Lys Ala Leu Tyr Lys Glu Leu Asp Leu Glu Gly Ala Phe Met 
    290                 295                 300                 


Glu Tyr Glu Lys Glu Ser Tyr Glu Lys Leu Thr Lys Leu Ile Glu Ala 
305                 310                 315                 320 


His Gln Ser Lys Ala Ile Gln Ala Val Leu Lys Ser Phe Leu Ala Lys 
                325                 330                 335     


Ile Tyr Lys Arg Gln Lys 
            340         


<210>  7
<211>  72
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  7
atgttgtcac tacgtcaatc tataagattt ttcaagccag ccacaagaac tttgtgtagc       60

tctcgttatc tg                                                           72


<210>  8
<211>  289
<212>  DNA
<213>  Ch. morifolium

<400>  8
atggcctcga tctcttcctc cgctgtcgca accgtcaaca ggaccacctc tgctcaagct       60

agcatggtgg ctccattcac cgggcttaag tccaacgtcg ctttcccagt caccaagaag      120

tctaacgact tctcatccct ccccagcaac ggtggaagag tgcaatgcat gaaggtacaa      180

tatataactt aaataataac gtgaacactt attataatgc agtagatata atgactaaca      240

ttttataaaa tatatatata ggtgtggcca ccattgggtt tgaagaagt                  289


