                         SEQUENCE LISTING

<110>  Bio-Nano Consulting Ltd
 
<120>  Modified arsenite oxidase and a biosensor for detecting arsenite  

<130>  APC00310WO

<160>  10     

<170>  PatentIn version 3.3

<210>  1
<211>  175
<212>  PRT
<213>  Rhizobium sp. NT-26

<400>  1

Met Ser Arg Cys Gln Asn Met Val Asp Ile Gly Arg Arg Gln Phe Leu 
1               5                   10                  15      


Arg Gly Gly Ala Leu Ala Ala Ala Gly Ala Thr Ala Ala Val Phe Gly 
            20                  25                  30          


Val Gly Ala Pro Gln Ala Arg Ala Ala Thr Ala Ala Ala Gly Val Glu 
        35                  40                  45              


Tyr Pro Ala Asn Arg Leu Ala Asn Ile Ser Glu Leu Thr Leu Asn Glu 
    50                  55                  60                  


Pro Leu Asp Val Ala Tyr Pro Asp Glu Asp Ala Ala Gly Val Leu Leu 
65                  70                  75                  80  


Lys Leu Gly Thr Arg Val Glu Gly Gly Val Gly Pro Asp Gly Asp Ile 
                85                  90                  95      


Val Gly Phe Ser Thr Ile Cys Pro His Lys Gly Phe Pro Leu Ser Tyr 
            100                 105                 110         


Ser Ala Asp Asn Lys Thr Phe Asn Cys Pro Gly His Phe Ser Val Phe 
        115                 120                 125             


Asp Pro Glu Lys Gly Gly Gln Gln Val Trp Gly Gln Ala Thr Gln Asn 
    130                 135                 140                 


Leu Pro Gln Tyr Val Leu Arg Val Ala Asp Asn Gly Asp Ile Phe Ala 
145                 150                 155                 160 



Glu Gly Val Asp Glu Leu Ile Tyr Gly Arg Leu Ser Asn Val Leu 
                165                 170                 175 


<210>  2
<211>  40
<212>  PRT
<213>  Rhizobium sp. NT-26

<400>  2

Met Ser Arg Cys Gln Asn Met Val Asp Ile Gly Arg Arg Gln Phe Leu 
1               5                   10                  15      


Arg Gly Gly Ala Leu Ala Ala Ala Gly Ala Thr Ala Ala Val Phe Gly 
            20                  25                  30

Val Gly Ala Pro Gln Ala Arg Ala
        35                  40
 

<210>  3
<211>  150
<212>  PRT
<213>  Rhizobium sp. NT-26

<400>  3

Ala Thr Ala Ala Val Phe Gly Val Gly Ala Pro Gln Ala Arg Ala Ala 
1               5                   10                  15      


Thr Ala Ala Ala Gly Val Glu Tyr Pro Ala Asn Arg Leu Ala Asn Ile 
            20                  25                  30          


Ser Glu Leu Thr Leu Asn Glu Pro Leu Asp Val Ala Tyr Pro Asp Glu 
        35                  40                  45              


Asp Ala Ala Gly Val Leu Leu Lys Leu Gly Thr Arg Val Glu Gly Gly 
    50                  55                  60                  


Val Gly Pro Asp Gly Asp Ile Val Gly Phe Ser Thr Ile Cys Pro His 
65                  70                  75                  80  


Lys Gly Phe Pro Leu Ser Tyr Ser Ala Asp Asn Lys Thr Phe Asn Cys 
                85                  90                  95      


Pro Gly His Phe Ser Val Phe Asp Pro Glu Lys Gly Gly Gln Gln Val 
            100                 105                 110         


Trp Gly Gln Ala Thr Gln Asn Leu Pro Gln Tyr Val Leu Arg Val Ala 
        115                 120                 125             




Asp Asn Gly Asp Ile Phe Ala Glu Gly Val Asp Glu Leu Ile Tyr Gly 
    130                 135                 140                 


Arg Leu Ser Asn Val Leu 
145                 150 


<210>  4
<211>  845
<212>  PRT
<213>  Rhizobium sp. NT-26

<400>  4

Met Ala Phe Lys Arg His Ile Asp Arg Leu Pro Ile Ile Pro Ala Asp 
1               5                   10                  15      


Ala Lys Lys His Asn Val Thr Cys His Phe Cys Ile Val Gly Cys Gly 
            20                  25                  30          


Tyr His Ala Tyr Thr Trp Pro Ile Asn Lys Gln Gly Gly Thr Asp Pro 
        35                  40                  45              


Gln Asn Asn Ile Phe Gly Val Asp Leu Ser Glu Gln Gln Gln Ala Glu 
    50                  55                  60                  


Ser Asp Ala Trp Tyr Ser Pro Ser Met Tyr Asn Val Val Lys Gln Asp 
65                  70                  75                  80  


Gly Arg Asp Val His Val Val Ile Lys Pro Asp His Glu Cys Val Val 
                85                  90                  95      


Asn Ser Gly Leu Gly Ser Val Arg Gly Ala Arg Met Ala Glu Thr Ser 
            100                 105                 110         


Phe Ser Glu Ala Arg Asn Thr Gln Gln Gln Arg Leu Thr Asp Pro Leu 
        115                 120                 125             


Val Trp Arg Tyr Gly Gln Met Gln Pro Thr Ser Trp Asp Asp Ala Leu 
    130                 135                 140                 


Asp Leu Val Ala Arg Val Thr Ala Lys Ile Val Lys Glu Lys Gly Glu 
145                 150                 155                 160 


Asp Ala Leu Ile Val Ser Ala Phe Asp His Gly Gly Ala Gly Gly Gly 
                165                 170                 175     



Tyr Glu Asn Thr Trp Gly Thr Gly Lys Leu Tyr Phe Glu Ala Met Lys 
            180                 185                 190         


Val Lys Asn Ile Arg Ile His Asn Arg Pro Ala Tyr Asn Ser Glu Val 
        195                 200                 205             


His Gly Thr Arg Asp Met Gly Val Gly Glu Leu Asn Asn Cys Tyr Glu 
    210                 215                 220                 


Asp Ala Glu Leu Ala Asp Thr Ile Val Ala Val Gly Thr Asn Ala Leu 
225                 230                 235                 240 


Glu Thr Gln Thr Asn Tyr Phe Leu Asn His Trp Ile Pro Asn Leu Arg 
                245                 250                 255     


Gly Glu Ser Leu Gly Lys Lys Lys Glu Leu Met Pro Glu Glu Pro His 
            260                 265                 270         


Glu Ala Gly Arg Ile Ile Ile Val Asp Pro Arg Arg Thr Val Thr Val 
        275                 280                 285             


Asn Ala Cys Glu Gln Thr Ala Gly Ala Asp Asn Val Leu His Leu Ala 
    290                 295                 300                 


Ile Asn Ser Gly Thr Asp Leu Ala Leu Phe Asn Ala Leu Phe Thr Tyr 
305                 310                 315                 320 


Ile Ala Asp Lys Gly Trp Val Asp Arg Asp Phe Ile Asp Lys Ser Thr 
                325                 330                 335     


Leu Arg Glu Gly Thr Ala Arg Pro Pro Leu Tyr Pro Ala Arg Gly Val 
            340                 345                 350         


Ser Glu Ala Asn Pro Gly His Leu Ser Ser Phe Glu Asp Ala Val Glu 
        355                 360                 365             


Gly Cys Arg Met Ser Ile Glu Glu Ala Ala Glu Ile Thr Gly Leu Asp 
    370                 375                 380                 


Ala Ala Gln Ile Ile Lys Ala Ala Glu Trp Ile Gly Met Pro Lys Glu 
385                 390                 395                 400 


Gly Gly Lys Arg Arg Arg Val Met Phe Gly Tyr Glu Lys Gly Leu Ile 
                405                 410                 415     


Trp Gly Asn Asp Asn Tyr Arg Thr Asn Gly Ala Leu Val Asn Leu Ala 
            420                 425                 430         


Leu Ala Thr Gly Asn Ile Gly Arg Pro Gly Gly Gly Val Val Arg Leu 
        435                 440                 445             


Gly Gly His Gln Glu Gly Tyr Val Arg Pro Ser Asp Ala His Val Gly 
    450                 455                 460                 


Arg Pro Ala Ala Tyr Val Asp Gln Leu Leu Ile Gly Gly Gln Gly Gly 
465                 470                 475                 480 


Val His His Ile Trp Gly Cys Asp His Tyr Lys Thr Thr Leu Asn Ala 
                485                 490                 495     


His Glu Phe Lys Arg Val Tyr Lys Lys Arg Thr Asp Met Val Lys Asp 
            500                 505                 510         


Ala Met Ser Ala Ala Pro Tyr Gly Asp Arg Glu Ala Met Val Asn Ala 
        515                 520                 525             


Ile Val Asp Ala Ile Asn Gln Gly Gly Leu Phe Ala Val Asn Val Asp 
    530                 535                 540                 


Ile Ile Pro Thr Lys Ile Gly Glu Ala Cys His Val Ile Leu Pro Ala 
545                 550                 555                 560 


Ala Thr Ser Gly Glu Met Asn Leu Thr Ser Met Asn Gly Glu Arg Arg 
                565                 570                 575     


Met Arg Leu Thr Glu Arg Tyr Met Asp Pro Pro Gly Gln Ser Met Pro 
            580                 585                 590         


Asp Cys Leu Ile Ala Ala Arg Leu Ala Asn Thr Met Glu Arg Val Leu 
        595                 600                 605             


Thr Glu Met Gly Asp Val Gly Tyr Ala Ala Gln Phe Lys Gly Phe Asp 
    610                 615                 620                 


Trp Gln Thr Glu Glu Asp Ala Phe Met Asp Gly Tyr Asn Lys Asn Ala 
625                 630                 635                 640 


His Gly Gly Glu Phe Val Thr Tyr Glu Arg Leu Ser Ala Met Gly Thr 
                645                 650                 655     


Asn Gly Phe Gln Glu Pro Ala Thr Gly Phe Thr Asp Gly Lys Ile Glu 
            660                 665                 670         


Gly Thr Gln Arg Leu Tyr Thr Asp Gly Val Phe Ser Thr Asp Asp Gly 
        675                 680                 685             


Lys Ala Arg Phe Met Asp Ala Pro Trp Arg Gly Leu Gln Ala Pro Gly 
    690                 695                 700                 


Lys Gln Gln Gln Lys Asp Ser His Lys Tyr Leu Ile Asn Asn Gly Arg 
705                 710                 715                 720 


Ala Asn Val Val Trp Gln Ser Ala Tyr Leu Asp Gln Glu Asn Asp Phe 
                725                 730                 735     


Val Met Asp Arg Phe Pro Tyr Pro Phe Ile Glu Met Asn Pro Glu Asp 
            740                 745                 750         


Met Ala Glu Ala Gly Leu Lys Glu Gly Asp Leu Val Glu Ile Tyr Asn 
        755                 760                 765             


Asp Ala Gly Ala Thr Gln Ala Met Ala Tyr Pro Thr Pro Thr Ala Arg 
    770                 775                 780                 


Arg Gly Glu Thr Phe Met Leu Phe Gly Phe Pro Thr Gly Val Gln Gly 
785                 790                 795                 800 


Asn Val Thr Ser Ala Gly Thr Asn Glu Leu Ile Ile Pro Asn Tyr Lys 
                805                 810                 815     


Gln Thr Trp Gly Asn Ile Arg Lys Ile Ser Asp Ala Pro Arg Asn Val 
            820                 825                 830         


Ala His Leu Ser Phe Lys Ser Lys Glu Tyr Gln Ser Ala 
        835                 840                 845 


<210>  5
<211>  528
<212>  DNA
<213>  Rhizobium sp. NT-26

<400>  5
atgtcacgtt gtcaaaacat ggtcgatatt ggccgacgcc agttcctgcg tggaggcgcg     60

ctcgcggctg cgggtgcgac tgccgccgtc ttcggcgtcg gcgcaccaca ggctagagcc    120

gctaccgcgg cggcaggggt cgaatatcct gccaatcgtc tggcaaacat ctcagaactt    180

acgctcaatg aaccgctcga tgtcgcctat ccggacgagg atgccgcagg cgttctgctt    240

aagcttggga cccgcgtcga gggtggcgtt ggccctgacg gcgacattgt cggcttttcc    300

acgatctgtc ctcacaaggg ttttcctctg agctactccg ccgacaacaa gacgttcaac    360

tgtcctggtc acttctcggt cttcgaccct gaaaagggcg gccagcaggt ttggggtcag    420

gccacgcaga acctgccgca atacgtgctc cgcgtcgccg acaatggcga catctttgcc    480

gaaggcgtcg acgagctgat ctacggccgt ctgtccaacg ttctataa                 528


<210>  6
<211>  120
<212>  DNA
<213>  Rhizobium sp. NT-26

<400>  6
atgtcacgtt gtcaaaacat ggtcgatatt ggccgacgcc agttcctgcg tggaggcgcg     60

ctcgcggctg cgggtgcgac tgccgccgtc ttcggcgtcg gcgcaccaca ggctagagcc    120


<210>  7
<211>  408
<212>  DNA
<213>  Rhizobium sp. NT-26

<400>  7
gctaccgcgg cggcaggggt cgaatatcct gccaatcgtc tggcaaacat ctcagaactt     60

acgctcaatg aaccgctcga tgtcgcctat ccggacgagg atgccgcagg cgttctgctt    120

aagcttggga cccgcgtcga gggtggcgtt ggccctgacg gcgacattgt cggcttttcc    180

acgatctgtc ctcacaaggg ttttcctctg agctactccg ccgacaacaa gacgttcaac    240

tgtcctggtc acttctcggt cttcgaccct gaaaagggcg gccagcaggt ttggggtcag    300

gccacgcaga acctgccgca atacgtgctc cgcgtcgccg acaatggcga catctttgcc    360

gaaggcgtcg acgagctgat ctacggccgt ctgtccaacg ttctataa                 408


<210>  8
<211>  2538
<212>  DNA
<213>  Rhizobium sp. NT-26

<400>  8
atggccttca aacgtcacat cgaccgtctg ccgatcattc ccgcggacgc caagaagcac     60

aatgtcacct gccacttctg catcgtcggt tgcggctatc acgcctatac ctggccgatc    120

aacaaacaag gcggtacgga tccacagaac aacatcttcg gcgtcgacct gtctgaacag    180

cagcaggcgg aaagcgacgc ctggtattca ccgtccatgt acaacgtggt caagcaggat    240

ggccgcgacg ttcatgtcgt catcaagcca gaccacgaat gtgtcgtgaa ctccggtctc    300

ggttcggtgc gtggcgcccg catggcagag acgagcttct cagaggcccg caacacccag    360

cagcagcgcc tcaccgatcc gcttgtctgg cgatacgggc agatgcaacc gacgagctgg    420

gacgacgcgc tcgatctcgt cgctcgcgtg accgcgaaga tcgtcaaaga gaagggtgag    480

gacgccctca tcgtatcggc ctttgaccat ggcggtgcag gcggcggcta cgagaacacc    540

tggggcacgg gaaagctcta tttcgaggcc atgaaggtca agaacatccg catccacaac    600

cgcccggcct acaattccga ggttcacggc acccgcgaca tgggcgtcgg cgagttgaat    660

aactgctacg aggatgccga actggccgac acgatcgttg cggttggcac caacgcgctg    720

gagacccaga ccaactactt cctaaatcac tggattccga atctgcgcgg cgaaagcctc    780

ggcaagaaaa aggagctcat gccggaggag ccccatgaag caggcaggat cattatcgtc    840

gatccgcgcc gcaccgtgac ggtcaatgcc tgcgagcaga cggccggcgc cgacaatgtc    900

ctgcatcttg ctatcaattc tggcacggac ctcgcccttt tcaacgcact cttcacctat    960

atcgccgaca agggctgggt cgatcgcgac ttcatcgaca agtcgacact gcgcgagggt   1020

acagcccgac cgccgctcta tcctgcccgt ggagtgtcag aggccaatcc ggggcatctc   1080

tcgagtttcg aggacgccgt ggaaggctgc cgcatgtcta tcgaggaggc tgcggaaatc   1140

accggtctcg acgccgccca gatcatcaag gcagccgagt ggatcggcat gcccaaggaa   1200

ggcggcaagc gccgccgtgt catgttcggt tacgagaagg gtctgatctg gggcaatgac   1260

aactaccgaa ccaacggcgc gctggtgaac ctcgcccttg ccaccggcaa tatcggccgt   1320

cccggtggcg gcgtcgtacg ccttggcgga caccaggaag gctatgtgcg cccctccgac   1380

gcccatgtcg gccggccggc ggcctatgtc gaccagttgc tgatcggcgg ccagggcggc   1440

gttcaccaca tctggggctg cgaccactac aaaacgacgc tcaatgcgca tgagtttaag   1500

cgcgtctaca agaagcgcac cgacatggtg aaggacgcca tgagcgctgc cccctacggc   1560

gaccgcgagg ccatggtcaa tgccattgtc gacgcaatca atcagggcgg attgtttgcc   1620

gtcaatgtcg acatcatccc gacaaaaatc ggcgaagcct gtcatgttat tctgcctgcg   1680

gccacgtcag gcgagatgaa cctcacgtca atgaatggcg agcggcgcat gcggctgacc   1740

gaacgctata tggacccgcc cggtcagtcc atgccggact gcctgattgc cgctcgtctc   1800

gccaacacca tggaacgcgt gctgaccgag atgggtgacg tcggctatgc cgctcaattc   1860

aagggctttg actggcagac agaagaagac gccttcatgg acggctacaa caagaatgca   1920

catggcggag agttcgtcac ctatgagcgc ctgagtgcga tgggcaccaa cggcttccag   1980

gagccggcta ccggctttac cgacggcaag atcgagggca cccagcggct ctataccgac   2040

ggcgtattct cgaccgacga cggcaaggcg cggttcatgg acgcgccatg gcggggactt   2100

caggcaccgg gcaagcagca gcagaaggac agccacaagt acttgatcaa caacggccgt   2160

gccaatgtcg tctggcaatc ggcgtatctc gaccaggaaa acgacttcgt catggatcgt   2220

ttcccctacc cgttcatcga gatgaaccca gaggacatgg cggaagcagg ccttaaggag   2280

ggcgacctcg tcgagattta caatgatgcc ggagcgacgc aggccatggc ctatccgacg   2340

ccgacagccc gacgtggaga aaccttcatg ctgttcggtt ttccaaccgg ggttcagggc   2400

aatgtgacca gtgccgggac gaacgagttg ataatcccga actacaagca gacctggggc   2460

aatatccgca agatttcgga tgcgcccagg aacgtggctc acctttcctt caagtcgaaa   2520

gaataccagt cggcttga                                                 2538


<210>  9
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer sequence

<400>  9
gcgaattcaa gctaccgcgg cggcaggggt c                                    31


<210>  10
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic primer sequence

<400>  10
gcctgcagtc aagccgactg gtattctttc ga                                   32





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