                         SEQUENCE LISTING

<110>  University of Toronto
 
<120>  NOVEL PROTEIN

<130>  K8000899PCT

<140>  PCT/CA2012/000951
<141>  2012-10-12

<150>  US 61/546,333
<151>  2011-10-12

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  1
ttgtaacaga ggcagagctg                                                   20


<210>  2
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  2
agtttcccgt ccttgttcg                                                    19


<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  3
ctgtgagcca tcaagctgtt g                                                 21


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  4
ggtgtacatg gcatccgatt t                                                 21


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  5
agccatcctt cttgggtatg                                                   20


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  6
agtttcccgt ccttgttcg                                                    19


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  7
agccatcctt cttgggtatg                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  8
atacctggga acatggtggt                                                   20


<210>  9
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  9
accttgactg tgctaaggta gcataa                                            26


<210>  10
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  10
cagttcttcc ctattaatcc gttcat                                            26


<210>  11
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA sequence

<400>  11
gaaacuuuca cuugaugaau ucaag                                             25


<210>  12
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA sequence

<400>  12
ccacguucga cgaggucaag aacua                                             25


<210>  13
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA

<400>  13
uggacacugu ccaggguuau uacau                                             25


<210>  14
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA

<400>  14
uuaaugaugc cauugguuag auaua                                             25


<210>  15
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA

<400>  15
ucacaaggga gagaaagaga ggaagga                                           27


<210>  16
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  16

Met Glu Arg Ala Phe Glu Asp Val Arg Ser Gln His Arg Asp Cys 
1               5                   10                  15  


<210>  17
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  17

Cys Asp Gly Lys Leu Ser Leu Asp Glu Phe Lys Thr Leu Tyr Ser Pro 
1               5                   10                  15      


<210>  18
<211>  179
<212>  PRT
<213>  snail

<400>  18

Met Glu Arg Ala Phe Glu Asp Val Arg Ser Gln His Arg Asp Ala Ser 
1               5                   10                  15      


Leu His Asn Val Leu Ser Arg Gly Thr Arg Ser Ala Asn Gly Gly Val 
            20                  25                  30          


Pro Cys Val Thr Val Pro Phe Leu Thr Glu Leu Lys Glu Arg Phe Ile 
        35                  40                  45              


Arg Trp Leu Asp His Asp Asn Asp Gly Gln Ser Thr Phe Asp Glu Val 
    50                  55                  60                  


Lys Asn Tyr Ile Arg Arg Phe Lys Pro Asp Val Thr Asp Gln Thr Val 
65                  70                  75                  80  


Ala Ala Phe Ile Ser Arg Arg Asp Ser Asn Gly Asn Gly Ala Ile Asp 
                85                  90                  95      


Phe Val Pro Glu Tyr Val His Asp Met Ala Ala Pro Asp Tyr Thr Leu 
            100                 105                 110         


Glu Gly Ala Asn Glu Trp Phe Lys Leu Gln Asp Thr Asn Asp Asp Ser 
        115                 120                 125             


Phe Val Thr Glu Ala Glu Leu Val Lys Val Ala Glu Ala Val Gly Met 
    130                 135                 140                 


Ser Pro Glu Glu Ala Leu Asp Thr Val Gln Gly Tyr Tyr Met Ser Ala 
145                 150                 155                 160 


Asp Ala Asn Lys Asp Gly Lys Leu Ser Leu Asp Glu Phe Lys Thr Leu 
                165                 170                 175     


Tyr Ser Pro 
            


<210>  19
<211>  537
<212>  DNA
<213>  snail

<400>  19
atggagaggg ccttcgagga cgtcaggagt cagcacaggg acgcgtcact gcacaacgtg       60

ctgagcaggg gcacccggag cgccaatggc ggcgtgccat gcgtgaccgt tccattttta      120

acagaactga aggagcgctt catccgctgg ctggaccacg acaacgacgg ccagtccacg      180

ttcgacgagg tcaagaacta catcagacgc tttaagcctg acgtcacgga ccagacggtg      240

gccgctttca tcagtcgccg agacagcaac gggaacggcg ccatagactt cgtccccgag      300

tacgtccacg acatggcggc accagactac acgctcgagg gcgccaacga gtggtttaaa      360

ctccaggaca ccaacgatga cagctttgta acagaggcag agctggtcaa ggtggcagag      420

gctgtcggca tgtccccaga ggaggcactg gacactgtcc agggttatta catgtccgcc      480

gatgcgaaca aggacgggaa actttcactt gatgaattca agacactgta cagccct         537


<210>  20
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  20
gacgacgaca agatgctggt tcctcgtggt tgg                                    33


<210>  21
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  21
gaggagaagc cggttagcga cgacgtttct tacg                                   34


<210>  22
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-6 tag

<400>  22

Met Ala His His His His His His 
1               5               


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker region

<400>  23

Val Asp Asp Asp Asp Lys Met 
1               5           


<210>  24
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  thrombin cleavage site

<400>  24

Leu Val Pro Arg Gly Ser 
1               5       


<210>  25
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HIV TAT protein transduction domain

<400>  25

Tyr Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg 
1               5                   10      


<210>  26
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  caltubin-TAT protein

<400>  26

Met Ala His His His His His His Val Asp Asp Asp Asp Lys Met Leu 
1               5                   10                  15      


Val Pro Arg Gly Ser Gly Ala Met Glu Arg Ala Phe Glu Asp Val Arg 
            20                  25                  30          


Ser Gln His Arg Asp Ala Ser Leu His Asn Val Leu Ser Arg Gly Thr 
        35                  40                  45              


Arg Ser Ala Asn Gly Gly Val Pro Cys Val Thr Val Pro Phe Leu Thr 
    50                  55                  60                  


Glu Leu Lys Glu Arg Phe Ile Arg Trp Leu Asp His Asp Asn Asp Gly 
65                  70                  75                  80  


Gln Ser Thr Phe Asp Glu Val Lys Asn Tyr Ile Arg Arg Phe Lys Pro 
                85                  90                  95      


Asp Val Thr Asp Gln Thr Val Ala Ala Phe Ile Ser Arg Arg Asp Ser 
            100                 105                 110         


Asn Gly Asn Gly Ala Ile Asp Phe Val Pro Glu Tyr Val His Asp Met 
        115                 120                 125             


Ala Ala Pro Asp Tyr Thr Leu Glu Gly Ala Asn Glu Trp Phe Lys Leu 
    130                 135                 140                 


Gln Asp Thr Asn Asp Asp Ser Phe Val Thr Glu Ala Glu Leu Val Lys 
145                 150                 155                 160 


Val Ala Glu Ala Val Gly Met Ser Pro Glu Glu Ala Leu Asp Thr Val 
                165                 170                 175     


Gln Gly Tyr Tyr Met Ser Ala Asp Ala Asn Lys Asp Gly Lys Leu Ser 
            180                 185                 190         


Leu Asp Glu Phe Lys Thr Leu Tyr Ser Pro Ala Ala Tyr Gly Arg Lys 
        195                 200                 205             


Lys Arg Arg Gln Arg Arg Arg 
    210                 215 


<210>  27
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal caltubin-TAT

<400>  27

Met Ala His His His His His His Val Asp Asp Asp Asp Lys Met Leu 
1               5                   10                  15      


Val Pro Arg Gly Ser Gly Ala Ala Ala Tyr Gly Arg Lys Lys Arg Arg 
            20                  25                  30          


Gln Arg Arg Arg 
        35      


<210>  28
<211>  103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal caltubin-TAT

<400>  28

Met Ala His His His His His His Val Asp Asp Asp Asp Lys Met Leu 
1               5                   10                  15      


Val Pro Arg Gly Ser Gly Ala Glu Gly Ala Asn Glu Trp Phe Lys Leu 
            20                  25                  30          


Gln Asp Thr Asn Asp Asp Ser Phe Val Thr Glu Ala Glu Leu Val Lys 
        35                  40                  45              


Val Ala Glu Ala Val Gly Met Ser Pro Glu Glu Ala Leu Asp Thr Val 
    50                  55                  60                  


Gln Gly Tyr Tyr Met Ser Ala Asp Ala Asn Lys Asp Gly Lys Leu Ser 
65                  70                  75                  80  


Leu Asp Glu Phe Lys Thr Leu Tyr Ser Pro Ala Ala Tyr Gly Arg Lys 
                85                  90                  95      


Lys Arg Arg Gln Arg Arg Arg 
            100             


