
<110>  Techlab, Inc.
 
<120>  CYSTEINE PROTEASE Cwp84 AS A DIAGNOSTIC MARKER FOR CLOSTRIDIUM 
       DIFFICILE

<130>  TLAB.163658

<150>  61539209
<151>  2011-09-26

<160>  1     

<170>  PatentIn version 3.5

<210>  1
<211>  803
<212>  PRT
<213>  Clostridium difficile strain 630


<220>
<221>  Cwp84
<222>  (1)..(803)


<400>  1

Met Arg Lys Tyr Lys Ser Lys Lys Leu Ser Lys Leu Leu Ala Leu Leu 
1               5                   10                  15      


Thr Val Cys Phe Leu Ile Val Ser Thr Ile Pro Val Ala Ala Glu Asn 
            20                  25                  30          


His Lys Thr Leu Asp Gly Val Glu Thr Ala Glu Tyr Ser Glu Ser Tyr 
        35                  40                  45              


Leu Gln Tyr Leu Glu Asp Val Lys Asn Gly Asp Thr Ala Lys Tyr Asn 
    50                  55                  60                  


Gly Val Ile Pro Phe Pro His Glu Met Glu Gly Thr Thr Leu Asn Asn 
65                  70                  75                  80  


Lys Gly Arg Ser Ser Leu Pro Ser Ala Tyr Lys Ser Ser Val Ala Tyr 
                85                  90                  95      


Asn Pro Met Asp Leu Gly Leu Thr Thr Pro Ala Lys Asn Gln Gly Ala 
            100                 105                 110         


Leu Asn Thr Cys Trp Ser Phe Ala Gly Met Ser Thr Leu Glu Ala Tyr 
        115                 120                 125             


Leu Lys Leu Lys Gly Tyr Gly Thr Tyr Asp Leu Ser Glu Glu His Leu 
    130                 135                 140                 


Arg Trp Trp Ala Thr Gly Gly Lys Tyr Gly Trp Asn Leu Asp Asp Met 
145                 150                 155                 160 


Ser Gly Ser Ser Asn Val Thr Ala Ile Gly Tyr Leu Thr Ala Trp Ala 
                165                 170                 175     


Gly Pro Lys Leu Glu Lys Asp Ile Pro Tyr Asn Leu Lys Ser Ala Ala 
            180                 185                 190         


Gln Gly Ala Thr Lys Pro Ser Asn Met Asp Thr Ala Pro Thr Gln Phe 
        195                 200                 205             


Asn Val Thr Asp Val Val Arg Leu Asn Lys Asp Lys Glu Thr Val Lys 
    210                 215                 220                 


Asn Ala Ile Met Gln Tyr Gly Ser Val Thr Ser Gln Tyr Ala His Tyr 
225                 230                 235                 240 


Ala Thr Tyr Phe Asn Lys Asp Glu Thr Ala Tyr Asn Cys Thr Asn Lys 
                245                 250                 255     


Arg Ala Pro Leu Asn His Ala Val Ala Ile Val Gly Trp Asp Asp Asn 
            260                 265                 270         


Tyr Ser Lys Asp Asn Phe Ala Ser Asp Tyr Lys Pro Glu Ser Asn Gly 
        275                 280                 285             


Ala Trp Leu Val Lys Ser Ser Trp Gly Glu Phe Asn Ser Met Lys Gly 
    290                 295                 300                 


Phe Phe Trp Ile Ser Tyr Glu Asp Lys Thr Leu Leu Thr Asp Thr Asp 
305                 310                 315                 320 


Asn Tyr Ala Met Lys Ser Val Ser Lys Pro Asp Ser Asp Lys Lys Met 
                325                 330                 335     


Tyr Gln Leu Glu Tyr Ala Gly Leu Ser Lys Ile Met Ser Asn Lys Val 
            340                 345                 350         


Thr Ala Ala Asn Val Phe Asp Phe Ser Arg Asp Ser Glu Lys Leu Asp 
        355                 360                 365             


Ser Val Met Phe Glu Thr Asp Ser Val Gly Ala Lys Tyr Glu Val Tyr 
    370                 375                 380                 


Tyr Ala Pro Val Val Asn Gly Val Pro Gln Asn Asn Ser Met Thr Lys 
385                 390                 395                 400 


Leu Ala Ser Gly Thr Val Ser Tyr Ser Gly Tyr Ile Asn Val Pro Thr 
                405                 410                 415     


Asn Ser Tyr Ser Leu Pro Lys Gly Lys Gly Ala Ile Val Val Val Ile 
            420                 425                 430         


Asp Asn Thr Ala Asn Pro Asn Arg Glu Lys Ser Thr Leu Ala Tyr Glu 
        435                 440                 445             


Thr Asn Ile Asp Ala Tyr Tyr Leu Tyr Glu Ala Lys Ala Asn Leu Gly 
    450                 455                 460                 


Glu Ser Tyr Ile Leu Gln Asn Asn Lys Phe Glu Asp Ile Asn Thr Tyr 
465                 470                 475                 480 


Ser Glu Phe Ser Pro Cys Asn Phe Val Ile Lys Ala Ile Thr Lys Thr 
                485                 490                 495     


Ser Ser Gly Gln Ala Thr Ser Gly Glu Ser Leu Thr Gly Ala Asp Arg 
            500                 505                 510         


Tyr Glu Thr Ala Val Lys Val Ser Gln Lys Gly Trp Thr Ser Ser Gln 
        515                 520                 525             


Asn Ala Val Leu Val Asn Gly Asp Ala Ile Val Asp Ala Leu Thr Ala 
    530                 535                 540                 


Thr Pro Phe Thr Ala Ala Ile Asp Ser Pro Ile Leu Leu Thr Gly Lys 
545                 550                 555                 560 


Asp Asn Leu Asp Ser Lys Thr Lys Ala Glu Leu Gln Arg Leu Gly Thr 
                565                 570                 575     


Lys Lys Val Tyr Leu Ile Gly Gly Glu Asn Ser Leu Ser Lys Asn Val 
            580                 585                 590         


Gln Thr Gln Leu Ser Asn Met Gly Ile Ser Val Glu Arg Ile Ser Gly 
        595                 600                 605             


Ser Asp Arg Tyr Lys Thr Ser Ile Ser Leu Ala Gln Lys Leu Asn Ser 
    610                 615                 620                 


Ile Lys Ser Val Ser Gln Val Ala Val Ala Asn Gly Val Asn Gly Leu 
625                 630                 635                 640 


Ala Asp Ala Ile Ser Val Gly Ala Ala Ala Ala Asp Asn Asn Met Pro 
                645                 650                 655     


Ile Ile Leu Thr Asn Glu Lys Ser Glu Leu Gln Gly Ala Asp Glu Phe 
            660                 665                 670         


Leu Asn Ser Ser Lys Ile Thr Lys Ser Tyr Ile Ile Gly Gly Thr Ala 
        675                 680                 685             


Thr Leu Ser Ser Asn Leu Glu Ser Lys Leu Ser Asn Pro Thr Arg Leu 
    690                 695                 700                 


Ala Gly Ser Asn Arg Asn Glu Thr Asn Ala Lys Ile Ile Asp Lys Phe 
705                 710                 715                 720 


Tyr Pro Ser Ser Asp Leu Lys Tyr Ala Phe Val Val Lys Asp Gly Ser 
                725                 730                 735     


Lys Ser Gln Gly Asp Leu Ile Asp Gly Leu Ala Val Gly Ala Leu Gly 
            740                 745                 750         


Ala Lys Thr Asp Ser Pro Val Val Leu Val Gly Asn Lys Leu Asp Glu 
        755                 760                 765             


Ser Gln Lys Asn Val Leu Lys Ser Lys Lys Ile Glu Thr Pro Ile Arg 
    770                 775                 780                 


Val Gly Gly Asn Gly Asn Glu Ser Ala Phe Asn Glu Leu Asn Thr Leu 
785                 790                 795                 800 


Leu Gly Lys 
            


