                         SEQUENCE LISTING

<110>  Gevo, Inc.
       The California Institute of Technology
       Buelter, Thomas
       Lies, Douglas
       Porter-Scheinman, Stephanie
       Smith, Christopher
       Meinhold, Peter
       Berry, Ruth
       Bastian, Sabine
       Snow, Christopher
 
<120>  KETOL-ACID REDUCTOISOMERASES WITH IMPROVED PERFORMANCE PROPERTIES

<130>  GEVO-063/02WO

<150>  US 61/497,851
<151>  2011-06-16

<150>  US 61/510,617
<151>  2011-07-22

<160>  88    

<170>  PatentIn version 3.5

<210>  1
<211>  1029
<212>  DNA
<213>  Slackia exigua

<400>  1
gtgtcggtca agactaagga gaaagaaatg gctgtcacca tcttgtacga acaggacgtc       60

gatcccaaag tcatacaggg cctcaaggtc ggcatcatcg gctacggctc ccagggccat      120

gcccatgcgc tgaacctcat ggattccggc gtcgacgtgc gcgtcggcct gcgcgaagga      180

tcctcttcct ggaagaccgc cgaagaggcc ggcctgaagg tcaccgacat ggacaccgcg      240

gccgaagaag ccgacgtcat catggtcctc gtccccgacg agatccagcc gaaggtctac      300

caggagcaca tcgccgcgca cctgaaggca ggcaacacgc tcgccttcgc ccatggcttc      360

aacatccact acggctacat cgtgccgccc gaggacgtca acgtcatcat gtgcgctccc      420

aagggcccgg ggcacatcgt ccgccgtcag ttcaccgaag gttccggcgt gcccgacctg      480

gcctgcgtcc agcaggatgc caccggcaac gcctgggata tcgtcctgtc ctactgctgg      540

ggcgtcggcg gggcccgttc cggcatcatc aaggcgacct tcgccgagga gaccgaggaa      600

gacctcttcg gcgagcaggc cgtgctctgc ggaggcctgg tggagctggt caaggccggc      660

ttcgagaccc tgaccgaggc agggtatccg cccgagctgg catacttcga gtgctatcac      720

gagatgaaga tgatcgtcga cctcatgtac gagagcggca tccacttcat gaactactcg      780

atctccaaca ccgccgaata cggcgagtac tacgccggcc cgaaggtcat caacgagcag      840

tcccgcgagg ccatgaagga gatcctgaag cgcattcagg acggctcctt cgcccaggaa      900

ttcgtcgacg actgcaacaa cggccataag cgcctgctcg agcagcgcga agcgatcaat      960

acgcacccca tcgagaccac cggcgcccag atccgcagca tgttctcctg gatcaagaag     1020

gaagactag                                                             1029


<210>  2
<211>  342
<212>  PRT
<213>  Slackia exigua

<400>  2

Met Ser Val Lys Thr Lys Glu Lys Glu Met Ala Val Thr Ile Leu Tyr 
1               5                   10                  15      


Glu Gln Asp Val Asp Pro Lys Val Ile Gln Gly Leu Lys Val Gly Ile 
            20                  25                  30          


Ile Gly Tyr Gly Ser Gln Gly His Ala His Ala Leu Asn Leu Met Asp 
        35                  40                  45              


Ser Gly Val Asp Val Arg Val Gly Leu Arg Glu Gly Ser Ser Ser Trp 
    50                  55                  60                  


Lys Thr Ala Glu Glu Ala Gly Leu Lys Val Thr Asp Met Asp Thr Ala 
65                  70                  75                  80  


Ala Glu Glu Ala Asp Val Ile Met Val Leu Val Pro Asp Glu Ile Gln 
                85                  90                  95      


Pro Lys Val Tyr Gln Glu His Ile Ala Ala His Leu Lys Ala Gly Asn 
            100                 105                 110         


Thr Leu Ala Phe Ala His Gly Phe Asn Ile His Tyr Gly Tyr Ile Val 
        115                 120                 125             


Pro Pro Glu Asp Val Asn Val Ile Met Cys Ala Pro Lys Gly Pro Gly 
    130                 135                 140                 


His Ile Val Arg Arg Gln Phe Thr Glu Gly Ser Gly Val Pro Asp Leu 
145                 150                 155                 160 


Ala Cys Val Gln Gln Asp Ala Thr Gly Asn Ala Trp Asp Ile Val Leu 
                165                 170                 175     


Ser Tyr Cys Trp Gly Val Gly Gly Ala Arg Ser Gly Ile Ile Lys Ala 
            180                 185                 190         


Thr Phe Ala Glu Glu Thr Glu Glu Asp Leu Phe Gly Glu Gln Ala Val 
        195                 200                 205             


Leu Cys Gly Gly Leu Val Glu Leu Val Lys Ala Gly Phe Glu Thr Leu 
    210                 215                 220                 


Thr Glu Ala Gly Tyr Pro Pro Glu Leu Ala Tyr Phe Glu Cys Tyr His 
225                 230                 235                 240 


Glu Met Lys Met Ile Val Asp Leu Met Tyr Glu Ser Gly Ile His Phe 
                245                 250                 255     


Met Asn Tyr Ser Ile Ser Asn Thr Ala Glu Tyr Gly Glu Tyr Tyr Ala 
            260                 265                 270         


Gly Pro Lys Val Ile Asn Glu Gln Ser Arg Glu Ala Met Lys Glu Ile 
        275                 280                 285             


Leu Lys Arg Ile Gln Asp Gly Ser Phe Ala Gln Glu Phe Val Asp Asp 
    290                 295                 300                 


Cys Asn Asn Gly His Lys Arg Leu Leu Glu Gln Arg Glu Ala Ile Asn 
305                 310                 315                 320 


Thr His Pro Ile Glu Thr Thr Gly Ala Gln Ile Arg Ser Met Phe Ser 
                325                 330                 335     


Trp Ile Lys Lys Glu Asp 
            340         


<210>  3
<211>  996
<212>  DNA
<213>  Cryptobacterium curtum

<400>  3
atggctgtca ccatcttgca cgaagaggat gcaaatccag caatcattca aggaaagaaa       60

attgccatta ttgggtatgg ttcacaaggg catgctcatg cgcttaatct gcatgattca      120

ggttgcgatg tacgtgttgg cctgcgccgt gattcatctt cttggaagat cgcagaagag      180

gctggtttga aagttatgac cactgctgaa gcggctcgcg aagccaatgt gattatgatt      240

ttgacacctg atgaaagtca gcgtgcggtg tacgctcaag atattgtccc gcatctgcaa      300

ccaggcgata cgcttgcctt tgcacatgga ttcaacatcc attttggata catcgtgccg      360

cccaaggatg ttaacgtcat catggtggcg ccaaaaggcc ctggtcatat tgtgcgtcgc      420

caatttactg aaggatccgg cgttcctgat cttatctgcg tgcagcaaga ctttaccgag      480

gatgcctggg atattgcctt atcgtatgcg tgggggctcg gcggcacgcg cgcgggggtt      540

attaagagta cctttaaaga cgaaactgaa gaggatctct ttggcgaaca ggcggtgctc      600

tgcggcgggg ttactcacct gatcgaagca ggatacgaag tgctgactga ggctggctat      660

ccgggtgagt tggcgtattt tgaggtgtac catgaaatga aaatgattgt ggacctcatg      720

tacgaaaacg gtatgcatgg tatgcgtagc tccatttcca atacagctga atatggtgac      780

tattacgctg gtccgcggat tattaccgaa gacaccaagg cagcgatgcg taccattctt      840

gcccgcattc aagatggatc gtttgcgaaa gagtttatcg acgattgcga tcgtggacat      900

gaagtgctgc tgagcaagcg tgctgaatat gcgaaccacg aaatcgaaca ggttggcgaa      960

gaaattcgca acatgtttag ctggatcaaa cgatag                                996


<210>  4
<211>  331
<212>  PRT
<213>  Cryptobacterium curtum

<400>  4

Met Ala Val Thr Ile Leu His Glu Glu Asp Ala Asn Pro Ala Ile Ile 
1               5                   10                  15      


Gln Gly Lys Lys Ile Ala Ile Ile Gly Tyr Gly Ser Gln Gly His Ala 
            20                  25                  30          


His Ala Leu Asn Leu His Asp Ser Gly Cys Asp Val Arg Val Gly Leu 
        35                  40                  45              


Arg Arg Asp Ser Ser Ser Trp Lys Ile Ala Glu Glu Ala Gly Leu Lys 
    50                  55                  60                  


Val Met Thr Thr Ala Glu Ala Ala Arg Glu Ala Asn Val Ile Met Ile 
65                  70                  75                  80  


Leu Thr Pro Asp Glu Ser Gln Arg Ala Val Tyr Ala Gln Asp Ile Val 
                85                  90                  95      


Pro His Leu Gln Pro Gly Asp Thr Leu Ala Phe Ala His Gly Phe Asn 
            100                 105                 110         


Ile His Phe Gly Tyr Ile Val Pro Pro Lys Asp Val Asn Val Ile Met 
        115                 120                 125             


Val Ala Pro Lys Gly Pro Gly His Ile Val Arg Arg Gln Phe Thr Glu 
    130                 135                 140                 


Gly Ser Gly Val Pro Asp Leu Ile Cys Val Gln Gln Asp Phe Thr Glu 
145                 150                 155                 160 


Asp Ala Trp Asp Ile Ala Leu Ser Tyr Ala Trp Gly Leu Gly Gly Thr 
                165                 170                 175     


Arg Ala Gly Val Ile Lys Ser Thr Phe Lys Asp Glu Thr Glu Glu Asp 
            180                 185                 190         


Leu Phe Gly Glu Gln Ala Val Leu Cys Gly Gly Val Thr His Leu Ile 
        195                 200                 205             


Glu Ala Gly Tyr Glu Val Leu Thr Glu Ala Gly Tyr Pro Gly Glu Leu 
    210                 215                 220                 


Ala Tyr Phe Glu Val Tyr His Glu Met Lys Met Ile Val Asp Leu Met 
225                 230                 235                 240 


Tyr Glu Asn Gly Met His Gly Met Arg Ser Ser Ile Ser Asn Thr Ala 
                245                 250                 255     


Glu Tyr Gly Asp Tyr Tyr Ala Gly Pro Arg Ile Ile Thr Glu Asp Thr 
            260                 265                 270         


Lys Ala Ala Met Arg Thr Ile Leu Ala Arg Ile Gln Asp Gly Ser Phe 
        275                 280                 285             


Ala Lys Glu Phe Ile Asp Asp Cys Asp Arg Gly His Glu Val Leu Leu 
    290                 295                 300                 


Ser Lys Arg Ala Glu Tyr Ala Asn His Glu Ile Glu Gln Val Gly Glu 
305                 310                 315                 320 


Glu Ile Arg Asn Met Phe Ser Trp Ile Lys Arg 
                325                 330     


<210>  5
<211>  996
<212>  DNA
<213>  Eggerthella sp.

<400>  5
atggctgtta cgatctatca cgagaaagac gcgaaccccc agctcatcca ggacaagaag       60

gtggccatca tcggctacgg cagccagggc cacgcccatg cgctcaacct gctggactcg      120

ggcgtggacg tgcgcgtggg cctgcgcgag gactcgcgct cgcgggccaa ggccgaggag      180

gcgggcctca aggtgatgag cgtcgccgac gctgccgagg aagccgactt catcatgatc      240

ctcacgcccg acgagaccca ggccgccacg tacgaagcgg acatcgcccc gcatctcaag      300

gccggcgaca cgctggcgtt cgcgcacggc ttcaacatcc acttcggcta catcgagccg      360

cccgcggacg tcgatgtggt catgatcgcg ccgaagggcc cgggccacat ggtgcgccgc      420

gtgttcaccg aaggcgcggg cgtgccgtgc ctcatctgcg tgaaccagga cgcgtcgggt      480

gcggccaagg atgtggcgct gtcgtacgcg tggggcatcg gcggcgcacg cgccggcgtc      540

atcgagacca cgttcaagaa cgagaccgaa accgacctgt tcggcgagca ggccgtgctg      600

tgcggcggcg tgacggcgct catcaacgcc ggcttcgaga cgctggtgga ggcgggctat      660

ccgcccgaga tggcctactt cgagtgcttc cacgagatga agctcatcgt ggacctcatg      720

tacgagggcg gcatgtcgaa catgcgctac tccatctcga acacggccga gtacggcgac      780

tactacgccg gcccgcaggt gatcaccgac gagacgaagg ccgcgatgaa gaccgtgctg      840

gcgcgcatcc aggacggcag cttcgcccac gagttcatgg aggactcgaa gaacggccag      900

aagtggctca aagagcagcg catggagcac ggcaacgccc agatcgagga agtgggcgcc      960

aacatccgct cgatgttcag cttcgtgagg aggtag                                996


<210>  6
<211>  331
<212>  PRT
<213>  Eggerthella sp.

<400>  6

Met Ala Val Thr Ile Tyr His Glu Lys Asp Ala Asn Pro Gln Leu Ile 
1               5                   10                  15      


Gln Asp Lys Lys Val Ala Ile Ile Gly Tyr Gly Ser Gln Gly His Ala 
            20                  25                  30          


His Ala Leu Asn Leu Leu Asp Ser Gly Val Asp Val Arg Val Gly Leu 
        35                  40                  45              


Arg Glu Asp Ser Arg Ser Arg Ala Lys Ala Glu Glu Ala Gly Leu Lys 
    50                  55                  60                  


Val Met Ser Val Ala Asp Ala Ala Glu Glu Ala Asp Phe Ile Met Ile 
65                  70                  75                  80  


Leu Thr Pro Asp Glu Thr Gln Ala Ala Thr Tyr Glu Ala Asp Ile Ala 
                85                  90                  95      


Pro His Leu Lys Ala Gly Asp Thr Leu Ala Phe Ala His Gly Phe Asn 
            100                 105                 110         


Ile His Phe Gly Tyr Ile Glu Pro Pro Ala Asp Val Asp Val Val Met 
        115                 120                 125             


Ile Ala Pro Lys Gly Pro Gly His Met Val Arg Arg Val Phe Thr Glu 
    130                 135                 140                 


Gly Ala Gly Val Pro Cys Leu Ile Cys Val Asn Gln Asp Ala Ser Gly 
145                 150                 155                 160 


Ala Ala Lys Asp Val Ala Leu Ser Tyr Ala Trp Gly Ile Gly Gly Ala 
                165                 170                 175     


Arg Ala Gly Val Ile Glu Thr Thr Phe Lys Asn Glu Thr Glu Thr Asp 
            180                 185                 190         


Leu Phe Gly Glu Gln Ala Val Leu Cys Gly Gly Val Thr Ala Leu Ile 
        195                 200                 205             


Asn Ala Gly Phe Glu Thr Leu Val Glu Ala Gly Tyr Pro Pro Glu Met 
    210                 215                 220                 


Ala Tyr Phe Glu Cys Phe His Glu Met Lys Leu Ile Val Asp Leu Met 
225                 230                 235                 240 


Tyr Glu Gly Gly Met Ser Asn Met Arg Tyr Ser Ile Ser Asn Thr Ala 
                245                 250                 255     


Glu Tyr Gly Asp Tyr Tyr Ala Gly Pro Gln Val Ile Thr Asp Glu Thr 
            260                 265                 270         


Lys Ala Ala Met Lys Thr Val Leu Ala Arg Ile Gln Asp Gly Ser Phe 
        275                 280                 285             


Ala His Glu Phe Met Glu Asp Ser Lys Asn Gly Gln Lys Trp Leu Lys 
    290                 295                 300                 


Glu Gln Arg Met Glu His Gly Asn Ala Gln Ile Glu Glu Val Gly Ala 
305                 310                 315                 320 


Asn Ile Arg Ser Met Phe Ser Phe Val Arg Arg 
                325                 330     


<210>  7
<211>  996
<212>  DNA
<213>  Eggerthella lenta

<400>  7
atggctgtta cgatctatca tgagaacgac gcgaaccccc agctcatcca ggacaagaaa       60

gtggccatca tcgggtacgg cagccagggc cacgcccatg cgctcaacct gaaagactcg      120

ggggtcgacg tgcgcgtggg cctgcgcgag gactcgaagt cgcgggtcaa ggccgaggag      180

gcgggcctca aggtgatgag cgtcgccgac gccgccgagg aagccgactt catcatgatc      240

ctcacgcccg acgagacgca ggccgcaacc tacgaggccg agatcgcccc gcatctcaag      300

cccggcgaca ccctcgcgtt cgcgcacggc ttcaacatcc acttcggcta catcacgccg      360

cccgaggacg tggacgtcgt catgatcgcg ccgaagggcc cgggccacat ggtgcgccgc      420

gtattcaccg agggcgcggg cgtgccgtgt ctcatctgcg tccagcagga cgcgtcgggc      480

cgggctaagg acgtggcgct gtcctacgcg tggggcatcg gcggcgcgcg cgccggcgtc      540

atcgagacca cgttcaagaa cgagacggaa accgacctgt tcggcgagca ggccgtgctg      600

tgcggcggcg tgacggccct catcaacgcc gggttcgaga cgctcgtcga ggcgggctac      660

ccgcccgaga tggcctactt cgagtgcttc cacgagatga agctcatcgt ggacctcatg      720

tacgaaggcg gcatgtcgaa catgcgctac tccatctcga acacggccga gtacggcgac      780

tactacgccg gcccccaggt gatcaccgac gacgcgaagg ccgcgatgaa gaccatcctc      840

gagcgcatcc aggacggcag cttcgcccac gagttcatgg aggactccaa gaacggccag      900

gcgtggctga aggagcagcg catggagcac ggcaacgccc agatcgagga agtgggcgcc      960

agcatccgct cgatgttcag cttcgtgagg aggtag                                996


<210>  8
<211>  331
<212>  PRT
<213>  Eggerthella lenta

<400>  8

Met Ala Val Thr Ile Tyr His Glu Asn Asp Ala Asn Pro Gln Leu Ile 
1               5                   10                  15      


Gln Asp Lys Lys Val Ala Ile Ile Gly Tyr Gly Ser Gln Gly His Ala 
            20                  25                  30          


His Ala Leu Asn Leu Lys Asp Ser Gly Val Asp Val Arg Val Gly Leu 
        35                  40                  45              


Arg Glu Asp Ser Lys Ser Arg Val Lys Ala Glu Glu Ala Gly Leu Lys 
    50                  55                  60                  


Val Met Ser Val Ala Asp Ala Ala Glu Glu Ala Asp Phe Ile Met Ile 
65                  70                  75                  80  


Leu Thr Pro Asp Glu Thr Gln Ala Ala Thr Tyr Glu Ala Glu Ile Ala 
                85                  90                  95      


Pro His Leu Lys Pro Gly Asp Thr Leu Ala Phe Ala His Gly Phe Asn 
            100                 105                 110         


Ile His Phe Gly Tyr Ile Thr Pro Pro Glu Asp Val Asp Val Val Met 
        115                 120                 125             


Ile Ala Pro Lys Gly Pro Gly His Met Val Arg Arg Val Phe Thr Glu 
    130                 135                 140                 


Gly Ala Gly Val Pro Cys Leu Ile Cys Val Gln Gln Asp Ala Ser Gly 
145                 150                 155                 160 


Arg Ala Lys Asp Val Ala Leu Ser Tyr Ala Trp Gly Ile Gly Gly Ala 
                165                 170                 175     


Arg Ala Gly Val Ile Glu Thr Thr Phe Lys Asn Glu Thr Glu Thr Asp 
            180                 185                 190         


Leu Phe Gly Glu Gln Ala Val Leu Cys Gly Gly Val Thr Ala Leu Ile 
        195                 200                 205             


Asn Ala Gly Phe Glu Thr Leu Val Glu Ala Gly Tyr Pro Pro Glu Met 
    210                 215                 220                 


Ala Tyr Phe Glu Cys Phe His Glu Met Lys Leu Ile Val Asp Leu Met 
225                 230                 235                 240 


Tyr Glu Gly Gly Met Ser Asn Met Arg Tyr Ser Ile Ser Asn Thr Ala 
                245                 250                 255     


Glu Tyr Gly Asp Tyr Tyr Ala Gly Pro Gln Val Ile Thr Asp Asp Ala 
            260                 265                 270         


Lys Ala Ala Met Lys Thr Ile Leu Glu Arg Ile Gln Asp Gly Ser Phe 
        275                 280                 285             


Ala His Glu Phe Met Glu Asp Ser Lys Asn Gly Gln Ala Trp Leu Lys 
    290                 295                 300                 


Glu Gln Arg Met Glu His Gly Asn Ala Gln Ile Glu Glu Val Gly Ala 
305                 310                 315                 320 


Ser Ile Arg Ser Met Phe Ser Phe Val Arg Arg 
                325                 330     


<210>  9
<211>  996
<212>  DNA
<213>  Eggerthella sp.

<400>  9
atggctgtca cgatctatca cgagaaagac gcgaaccccc agctcatcca ggacaagaag       60

gtggccatca tcggctacgg cagccagggc cacgcccatg cgctcaacct gctggactcg      120

ggcgtggacg tgcgcgtggg cctgcgcgag gactcgcgct cgcgggccaa ggccgaggag      180

gcgggcctca aggtgatgag cgtcgccgac gctgccgagg aagccgactt catcatgatc      240

ctcacgcccg acgagaccca ggccgccacg tacgaagcgg acatcgcccc gcatctcaag      300

gccggcgaca cgctggcgtt cgcgcacggc ttcaacatcc acttcggcta catcgagtcg      360

cccgcggagg tcgacgtggt catgatcgcg ccgaagggcc cgggccacat ggtgcgccgc      420

gtgttcaccg aaggcgcggg cgtgccgtgc ctcatctgcg tgaaccagga cgcgtcgggc      480

caggccaagg atgtggcgct gtcgtacgcg tggggcatcg gcggcgcgcg cgccggcgtc      540

atcgaaacca cgttcaagaa cgagaccgaa accgacctgt tcggcgagca ggccgtgctg      600

tgcggcggcg tgacggcgct catcaacgcc ggcttcgaga cgctggtgga ggcgggctat      660

ccgcccgaga tggcctactt cgagtgcttc cacgagatga agctcatcgt ggacctcatg      720

tacgagggcg gcatgtcgaa catgcgctac tccatctcga acacggccga gtacggcgac      780

tactacgccg gcccgcaggt gatcaccgac gagacgaaag ccgcgatgaa gaccgtcctc      840

gcgcgcatcc aggacggcag cttcgcccac gagttcatgg aggactcgaa gaacggccag      900

aagtggctca aagagcagcg catggagcac ggcaacgccc agatcgagga agtgggcgcc      960

aacatccgct cgatgttcag cttcgtgagg aggtag                                996


<210>  10
<211>  331
<212>  PRT
<213>  Eggerthella sp.

<400>  10

Met Ala Val Thr Ile Tyr His Glu Lys Asp Ala Asn Pro Gln Leu Ile 
1               5                   10                  15      


Gln Asp Lys Lys Val Ala Ile Ile Gly Tyr Gly Ser Gln Gly His Ala 
            20                  25                  30          


His Ala Leu Asn Leu Leu Asp Ser Gly Val Asp Val Arg Val Gly Leu 
        35                  40                  45              


Arg Glu Asp Ser Arg Ser Arg Ala Lys Ala Glu Glu Ala Gly Leu Lys 
    50                  55                  60                  


Val Met Ser Val Ala Asp Ala Ala Glu Glu Ala Asp Phe Ile Met Ile 
65                  70                  75                  80  


Leu Thr Pro Asp Glu Thr Gln Ala Ala Thr Tyr Glu Ala Asp Ile Ala 
                85                  90                  95      


Pro His Leu Lys Ala Gly Asp Thr Leu Ala Phe Ala His Gly Phe Asn 
            100                 105                 110         


Ile His Phe Gly Tyr Ile Glu Ser Pro Ala Glu Val Asp Val Val Met 
        115                 120                 125             


Ile Ala Pro Lys Gly Pro Gly His Met Val Arg Arg Val Phe Thr Glu 
    130                 135                 140                 


Gly Ala Gly Val Pro Cys Leu Ile Cys Val Asn Gln Asp Ala Ser Gly 
145                 150                 155                 160 


Gln Ala Lys Asp Val Ala Leu Ser Tyr Ala Trp Gly Ile Gly Gly Ala 
                165                 170                 175     


Arg Ala Gly Val Ile Glu Thr Thr Phe Lys Asn Glu Thr Glu Thr Asp 
            180                 185                 190         


Leu Phe Gly Glu Gln Ala Val Leu Cys Gly Gly Val Thr Ala Leu Ile 
        195                 200                 205             


Asn Ala Gly Phe Glu Thr Leu Val Glu Ala Gly Tyr Pro Pro Glu Met 
    210                 215                 220                 


Ala Tyr Phe Glu Cys Phe His Glu Met Lys Leu Ile Val Asp Leu Met 
225                 230                 235                 240 


Tyr Glu Gly Gly Met Ser Asn Met Arg Tyr Ser Ile Ser Asn Thr Ala 
                245                 250                 255     


Glu Tyr Gly Asp Tyr Tyr Ala Gly Pro Gln Val Ile Thr Asp Glu Thr 
            260                 265                 270         


Lys Ala Ala Met Lys Thr Val Leu Ala Arg Ile Gln Asp Gly Ser Phe 
        275                 280                 285             


Ala His Glu Phe Met Glu Asp Ser Lys Asn Gly Gln Lys Trp Leu Lys 
    290                 295                 300                 


Glu Gln Arg Met Glu His Gly Asn Ala Gln Ile Glu Glu Val Gly Ala 
305                 310                 315                 320 


Asn Ile Arg Ser Met Phe Ser Phe Val Arg Arg 
                325                 330     


<210>  11
<211>  1002
<212>  DNA
<213>  Slackia exigua

<400>  11
atggctgtca ccatcttgta cgaacaggac gtcgatccca aagtcataca gggcctcaag       60

gtcggcatca tcggctacgg ctcccagggc catgcccatg cgctgaacct catggattcc      120

ggcgtcgacg tgcgcgtcgg cctgcgcgaa ggatcctctt cctggaagac cgccgaagag      180

gccggcctga aggtcaccga catggacacc gcggccgaag aagccgacgt catcatggtc      240

ctcgtccccg acgagatcca gccgaaggtc taccaggagc acatcgccgc gcacctgaag      300

gcaggcaaca cgctcgcctt cgcccatggc ttcaacatcc actacggcta catcgtgccg      360

cccgaggacg tcaacgtcat catgtgcgct cccaagggcc cggggcacat cgtccgccgt      420

cagttcaccg aaggttccgg cgtgcccgac ctggcctgcg tccagcagga tgccaccggc      480

aacgcctggg atatcgtcct gtcctactgc tggggcgtcg gcggggcccg ttccggcatc      540

atcaaggcga ccttcgccga ggagaccgag gaagacctct tcggcgagca ggccgtgctc      600

tgcggaggcc tggtggagct ggtcaaggcc ggcttcgaga ccctgaccga ggcagggtat      660

ccgcccgagc tggcatactt cgagtgctat cacgagatga agatgatcgt cgacctcatg      720

tacgagagcg gcatccactt catgaactac tcgatctcca acaccgccga atacggcgag      780

tactacgccg gcccgaaggt catcaacgag cagtcccgcg aggccatgaa ggagatcctg      840

aagcgcattc aggacggctc cttcgcccag gaattcgtcg acgactgcaa caacggccat      900

aagcgcctgc tcgagcagcg cgaagcgatc aatacgcacc ccatcgagac caccggcgcc      960

cagatccgca gcatgttctc ctggatcaag aaggaagact ag                        1002


<210>  12
<211>  333
<212>  PRT
<213>  Slackia exigua

<400>  12

Met Ala Val Thr Ile Leu Tyr Glu Gln Asp Val Asp Pro Lys Val Ile 
1               5                   10                  15      


Gln Gly Leu Lys Val Gly Ile Ile Gly Tyr Gly Ser Gln Gly His Ala 
            20                  25                  30          


His Ala Leu Asn Leu Met Asp Ser Gly Val Asp Val Arg Val Gly Leu 
        35                  40                  45              


Arg Glu Gly Ser Ser Ser Trp Lys Thr Ala Glu Glu Ala Gly Leu Lys 
    50                  55                  60                  


Val Thr Asp Met Asp Thr Ala Ala Glu Glu Ala Asp Val Ile Met Val 
65                  70                  75                  80  


Leu Val Pro Asp Glu Ile Gln Pro Lys Val Tyr Gln Glu His Ile Ala 
                85                  90                  95      


Ala His Leu Lys Ala Gly Asn Thr Leu Ala Phe Ala His Gly Phe Asn 
            100                 105                 110         


Ile His Tyr Gly Tyr Ile Val Pro Pro Glu Asp Val Asn Val Ile Met 
        115                 120                 125             


Cys Ala Pro Lys Gly Pro Gly His Ile Val Arg Arg Gln Phe Thr Glu 
    130                 135                 140                 


Gly Ser Gly Val Pro Asp Leu Ala Cys Val Gln Gln Asp Ala Thr Gly 
145                 150                 155                 160 


Asn Ala Trp Asp Ile Val Leu Ser Tyr Cys Trp Gly Val Gly Gly Ala 
                165                 170                 175     


Arg Ser Gly Ile Ile Lys Ala Thr Phe Ala Glu Glu Thr Glu Glu Asp 
            180                 185                 190         


Leu Phe Gly Glu Gln Ala Val Leu Cys Gly Gly Leu Val Glu Leu Val 
        195                 200                 205             


Lys Ala Gly Phe Glu Thr Leu Thr Glu Ala Gly Tyr Pro Pro Glu Leu 
    210                 215                 220                 


Ala Tyr Phe Glu Cys Tyr His Glu Met Lys Met Ile Val Asp Leu Met 
225                 230                 235                 240 


Tyr Glu Ser Gly Ile His Phe Met Asn Tyr Ser Ile Ser Asn Thr Ala 
                245                 250                 255     


Glu Tyr Gly Glu Tyr Tyr Ala Gly Pro Lys Val Ile Asn Glu Gln Ser 
            260                 265                 270         


Arg Glu Ala Met Lys Glu Ile Leu Lys Arg Ile Gln Asp Gly Ser Phe 
        275                 280                 285             


Ala Gln Glu Phe Val Asp Asp Cys Asn Asn Gly His Lys Arg Leu Leu 
    290                 295                 300                 


Glu Gln Arg Glu Ala Ile Asn Thr His Pro Ile Glu Thr Thr Gly Ala 
305                 310                 315                 320 


Gln Ile Arg Ser Met Phe Ser Trp Ile Lys Lys Glu Asp 
                325                 330             


<210>  13
<211>  7
<212>  PRT
<213>  Unknown

<220>
<223>  acetolactate synthase motif


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa may be Ala, Cys or Val

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa may be Thr or Ser

<400>  13

Ser Gly Pro Gly Xaa Xaa Asn 
1               5           


<210>  14
<211>  6
<212>  PRT
<213>  Unknown

<220>
<223>  acetolactate synthase motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa may be Pro or Ala

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa may be Val, Ala or Thr

<400>  14

Gly Xaa Xaa Gly Xaa Xaa 
1               5       


<210>  15
<211>  15
<212>  PRT
<213>  Unknown

<220>
<223>  acetolactate synthase motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa may be Gln or Gly

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa may be Thr or Ala

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa may be Leu or Met

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa may be Tyr, Phe or Trp

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Xaa may be Ala or Gly

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa may be Pro or Gly

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Xaa may be Trp or Ala

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Xaa may be Gly or Thr

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Xaa may be Ala or Val

<400>  15

Gly Xaa Xaa Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Ala Xaa Xaa Xaa 
1               5                   10                  15  


<210>  16
<211>  5
<212>  PRT
<213>  Unknown

<220>
<223>  acetolactate synthase motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa may be Gly or Ala

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa may be Gly, Ser or Cys

<400>  16

Gly Asp Xaa Xaa Phe 
1               5   


<210>  17
<211>  9
<212>  PRT
<213>  Unknown

<220>
<223>  dihydroxy acid dehydratase motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa may be any naturally occurring amino acid

<400>  17

Ser Leu Xaa Ser Arg Xaa Xaa Ile Ala 
1               5                   


<210>  18
<211>  7
<212>  PRT
<213>  Unknown

<220>
<223>  dihydroxy acid dehydratase motif


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Xaa may be any naturally occurring amino acid

<400>  18

Cys Asp Lys Xaa Xaa Pro Gly 
1               5           


<210>  19
<211>  10
<212>  PRT
<213>  Unknown

<220>
<223>  dihydroxy acid dehydratase motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa may be any naturally occurring amino acid

<400>  19

Gly Xaa Cys Xaa Gly Xaa Xaa Thr Ala Asn 
1               5                   10  


<210>  20
<211>  5
<212>  PRT
<213>  Unknown

<220>
<223>  dihydroxy acid dehydratase motif

<400>  20

Gly Gly Ser Thr Asn 
1               5   


<210>  21
<211>  11
<212>  PRT
<213>  Unknown

<220>
<223>  dihydroxy acid dehydratase motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa may be any naturally occurring amino acid

<400>  21

Gly Pro Xaa Gly Xaa Pro Gly Met Arg Xaa Glu 
1               5                   10      


<210>  22
<211>  10
<212>  PRT
<213>  Unknown

<220>
<223>  dihydroxy acid dehydratase motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Xaa may be any naturally occurring amino acid

<400>  22

Ala Leu Xaa Thr Asp Gly Arg Xaa Ser Gly 
1               5                   10  


<210>  23
<211>  7
<212>  PRT
<213>  Unknown

<220>
<223>  dihydroxy acid dehydratase motif


<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Xaa may be any naturally occurring amino acid

<400>  23

Gly His Xaa Xaa Pro Glu Ala 
1               5           


<210>  24
<211>  7
<212>  PRT
<213>  Unknown

<220>
<223>  2-keto-acid decarboxylase motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa may be Val or Ile

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa may be Pro or Ser


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa may be Asp or Glu

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa may be Tyr or Phe

<400>  24

Phe Gly Xaa Xaa Gly Xaa Xaa 
1               5           


<210>  25
<211>  9
<212>  PRT
<213>  Unknown

<220>
<223>  2-keto-acid decarboxylase motif


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa may be Thr or Val

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa may be Phe or Tyr

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa may be Val or Ala

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa may be Glu or Ala

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Xaa may be Leu or Phe

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Xaa may be Ser or Asn

<400>  25

Xaa Thr Xaa Gly Xaa Gly Xaa Xaa Xaa 
1               5                   


<210>  26
<211>  9
<212>  PRT
<213>  Unknown

<220>
<223>  2-keto-acid decarboxylase motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa may be Gly or Ala

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa may be Leu, Ile or Val

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa may be Ser or Ala

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa may be Tyr or Phe

<400>  26

Asn Xaa Xaa Ala Gly Xaa Xaa Ala Glu 
1               5                   


<210>  27
<211>  6
<212>  PRT
<213>  Unknown

<220>
<223>  2-keto-acid decarboxylase motif


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa may be Val or Ile

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa may be Leu, Ile or Val

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa may be Val, Thr or Ser

<400>  27

Xaa Xaa Xaa Ile Xaa Gly 
1               5       


<210>  28
<211>  8
<212>  PRT
<213>  Unknown

<220>
<223>  2-keto-acid decarboxylase motif


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa may be Ser or Ala

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa may be Leu or Phe or Ala

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa may be Leu or Met

<400>  28

Gly Asp Gly Xaa Xaa Gln Xaa Thr 
1               5               


<210>  29
<211>  6
<212>  PRT
<213>  Unknown

<220>
<223>  alcohol dehydrogenase motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa may be His or Gly

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa may be Thr or Ser

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa may be Leu or Ile

<400>  29

Cys Xaa Xaa Asp Xaa His 
1               5       


<210>  30
<211>  8
<212>  PRT
<213>  Unknown

<220>
<223>  alcohol dehydrogenase motif


<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa may be any naturally occurring amino acid

<400>  30

Gly His Glu Xaa Xaa Gly Xaa Val 
1               5               


<210>  31
<211>  7
<212>  PRT
<213>  Unknown

<220>
<223>  alcohol dehydrogenase motif


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa may be Leu or Val

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa may be Gln, Lys or Glu

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa may be Val, Ile or Lys

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa may be Asp or Gln

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa may be Arg or His

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa may be Val or Ala

<400>  31

Xaa Xaa Xaa Gly Xaa Xaa Xaa 
1               5           


<210>  32
<211>  7
<212>  PRT
<213>  Unknown

<220>
<223>  alcohol dehydrogenase motif


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  Xaa may be any naturally occurring amino acid

<400>  32

Cys Xaa Xaa Cys Xaa Xaa Cys 
1               5           


<210>  33
<211>  6
<212>  PRT
<213>  Unknown

<220>
<223>  alcohol dehydrogenase motif


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa may be Cys or Ala

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa may be Ala, Gly or Asp

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa may be Gly or Ala

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa may be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa may be Thr or Val

<400>  33

Xaa Xaa Xaa Xaa Thr Xaa 
1               5       


<210>  34
<211>  6
<212>  PRT
<213>  Unknown

<220>
<223>  alcohol dehydrogenase motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa may be Leu, Ala or Cys

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa may be Gly or Pro

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa may be Leu, Ile or Val

<400>  34

Gly Xaa Gly Xaa Xaa Gly 
1               5       


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer T7_for

<400>  35
taatacgact cactataggg                                                   20


<210>  36
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer T7_rev

<400>  36
gctagttatt gctcagcgg                                                    19


<210>  37
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_I33NNK_for


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<400>  37
ggtcttaaag ttggtatcnn kggttacggt tcccaaggt                              39


<210>  38
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_I33NNK_rev


<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or t

<400>  38
accttgggaa ccgtaaccmn ngataccaac tttaagacc                              39


<210>  39
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_G34NNK_for


<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<400>  39
aaagttggta tcatcnnkta cggttcccaa ggt                                    33


<210>  40
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_G34NNK_rev


<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  40
accttgggaa ccgtamnnga tgataccaac ttt                                    33


<210>  41
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_Y35NNK_for


<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<400>  41
gttggtatca tcggtnnkgg ttcccaaggt cac                                    33


<210>  42
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_Y35NNK_rev


<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  42
gtgaccttgg gaaccmnnac cgatgatacc aac                                    33


<210>  43
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_G36NNK_for


<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<400>  43
ggtatcatcg gttacnnktc ccaaggtcac gct                                    33


<210>  44
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_G36NNK_rev


<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  44
agcgtgacct tgggamnngt aaccgatgat acc                                    33


<210>  45
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_L57NNK_for


<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<400>  45
gatgttagag ttggcnnkag agaaggctca tct                                    33


<210>  46
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_L57NNK_rev


<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  46
agatgagcct tctctmnngc caactctaac atc                                    33


<210>  47
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_R58NNK_for


<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<400>  47
gttagagttg gcttannkga aggctcatct agt                                    33


<210>  48
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_R58NNK_rev


<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  48
actagatgag ccttcmnnta agccaactct aac                                    33


<210>  49
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_G60NNK_for


<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<400>  49
gttggcttaa gagaannktc atctagttgg aaa                                    33


<210>  50
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_G60NNK_rev


<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  50
tttccaacta gatgamnntt ctcttaagcc aac                                    33


<210>  51
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_S61NNK_for


<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<400>  51
ggcttaagag aaggcnnktc tagttggaaa acg                                    33


<210>  52
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_S61NNK_rev


<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  52
cgttttccaa ctagamnngc cttctcttaa gcc                                    33


<210>  53
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_S62NNK_for


<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<400>  53
ttaagagaag gctcannkag ttggaaaacg gct                                    33


<210>  54
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_S62NNK_rev


<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  54
agccgttttc caactmnntg agccttctct taa                                    33


<210>  55
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_S63NNK_for


<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<400>  55
agagaaggct catctnnktg gaaaacggct gag                                    33


<210>  56
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_S63NNK_rev


<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  56
ctcagccgtt ttccamnnag atgagccttc tct                                    33


<210>  57
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_L90NNK_for


<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<400>  57
gatgtcatca tggttnnkgt gcctgatgaa att                                    33


<210>  58
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_L90NNK_rev


<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  58
aatttcatca ggcacmnnaa ccatgatgac atc                                    33


<210>  59
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_I95NNK_for


<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<400>  59
ttggtgcctg atgaannkca acctaaggta tat                                    33


<210>  60
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_I95NNK_rev


<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  60
atatacctta ggttgmnntt catcaggcac caa                                    33


<210>  61
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_V99NNK_for


<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<400>  61
gaaattcaac ctaagnnkta tcaggaacat atc                                    33


<210>  62
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_V99NNK_rev


<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  62
gatatgttcc tgatamnnct taggttgaat ttc                                    33


<210>  63
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb1_Y35YAC_for

<400>  63
ggtatcatcg gtyacggttc ccaaggt                                           27


<210>  64
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb1_Y35YAC_rev

<400>  64
accttgggaa ccgtraccga tgatacc                                           27


<210>  65
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb2a_for

<400>  65
ggcttaagag aagkatsctc tagttggaaa acggct                                 36


<210>  66
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb2b_for

<400>  66
ggcttaagag aagkatsctc tgattggaaa acggct                                 36


<210>  67
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb2c_for

<400>  67
ggcttaagag aagkatsctc tcagtggaaa acggct                                 36


<210>  68
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb2a_rev

<400>  68
agccgttttc caactagags atmcttctct taagcc                                 36


<210>  69
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb2b_rev

<400>  69
agccgttttc caatcagags atmcttctct taagcc                                 36


<210>  70
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb2c_rev

<400>  70
agccgttttc cactgagags atmcttctct taagcc                                 36


<210>  71
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb3a_for

<400>  71
cctgatgaaa hccaacctaa gktatatcag gaa                                    33


<210>  72
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb3b_for

<400>  72
cctgatgaag yacaacctaa gktatatcag gaa                                    33


<210>  73
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb3a_rev

<400>  73
ttcctgatat amcttaggtt ggdtttcatc agg                                    33


<210>  74
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb3b_rev

<400>  74
ttcctgatat amcttaggtt gtrcttcatc agg                                    33


<210>  75
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb4a_L57TYA_for

<400>  75
gtcgatgtta gagttggcty aagagaa                                           27


<210>  76
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb4a_L57TYA_rev

<400>  76
ttctcttrag ccaactctaa catcgac                                           27


<210>  77
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb4b_L57CRA_for

<400>  77
gtcgatgtta gagttggccr aagagaa                                           27


<210>  78
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_recomb4b_L57CRA_rev

<400>  78
ttctcttygg ccaactctaa catcgac                                           27


<210>  79
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_L57NNK_R58P_S61TSC_for


<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  n is a, c, g, or t

<400>  79
agagttggcn nkccagaagg ctsctctagt tgg                                    33


<210>  80
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se_L57NNK_R58P_S61TSC_rev


<220>
<221>  misc_feature
<222>  (23)..(24)
<223>  n is a, c, g, or t

<400>  80
ccaactagag sagccttctg gmnngccaac tct                                    33


<210>  81
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Serec4_fancy3_rec_A_for

<400>  81
gttagagttg gcgtaccaga agkatgctct agttggaaa                              39


<210>  82
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Serec4_fancy3_rec_A_rev

<400>  82
tttccaacta gagcatmctt ctggtacgcc aactctaac                              39


<210>  83
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Serec4_fancy3_rec_B_for

<400>  83
cctgatgaar yacaacctaa gktatatcag gaacat                                 36


<210>  84
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Serec4_fancy3_rec_B_rev

<400>  84
atgttcctga tatamcttag gttgtryttc atcagg                                 36


<210>  85
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se1_S63D_S61D_for

<400>  85
ggcttaagag aaggcgactc tgactggaaa acggctgag                              39


<210>  86
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Se1_S63D_S61D_rev

<400>  86
ctcagccgtt ttccagtcag agtcgccttc tcttaagcc                              39


<210>  87
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer SeAA10R58S62NNK_for


<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n is a, c, g, or t

<400>  87
gttggcttan nkgaaggctg cnnkgattgg aaaacggct                              39


<210>  88
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer SeAA10R58S62NNK_rev


<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<400>  88
agccgttttc caatcmnngc agccttcmnn taagccaac                              39


