                         SEQUENCE LISTING

<110>  Iowa State University Research Foundation, Inc.
       Yang, Bing
       Li, Ting
       Huang, Sheng
 
<120>  Monomer Architecture of TAL Nuclease or Zinc Finger Nuclease for 
       Efficient  DNA  Modification

<130>  ISURF 3897

<150>  61/538,260
<151>  2011-09-23

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  245
<212>  PRT
<213>  Bacteriophage T4

<400>  1

Met Lys Ser Gly Ile Tyr Gln Ile Lys Asn Thr Leu Asn Asn Lys Val 
1               5                   10                  15      


Tyr Val Gly Ser Ala Lys Asp Phe Glu Lys Arg Trp Lys Arg His Phe 
            20                  25                  30          


Lys Asp Leu Glu Lys Gly Cys His Ser Ser Ile Lys Leu Gln Arg Ser 
        35                  40                  45              


Phe Asn Lys His Gly Asn Val Phe Glu Cys Ser Ile Leu Glu Glu Ile 
    50                  55                  60                  


Pro Tyr Glu Lys Asp Leu Ile Ile Glu Arg Glu Asn Phe Trp Ile Lys 
65                  70                  75                  80  


Glu Leu Asn Ser Lys Ile Asn Gly Tyr Asn Ile Ala Asp Ala Thr Phe 
                85                  90                  95      


Gly Asp Thr Cys Ser Thr His Pro Leu Lys Glu Glu Ile Ile Lys Lys 
            100                 105                 110         


Arg Ser Glu Thr Val Lys Ala Lys Met Leu Lys Leu Gly Pro Asp Gly 
        115                 120                 125             


Arg Lys Ala Leu Tyr Ser Lys Pro Gly Ser Lys Asn Gly Arg Trp Asn 
    130                 135                 140                 


Pro Glu Thr His Lys Phe Cys Lys Cys Gly Val Arg Ile Gln Thr Ser 
145                 150                 155                 160 


Ala Tyr Thr Cys Ser Lys Cys Arg Asn Arg Ser Gly Glu Asn Asn Ser 
                165                 170                 175     


Phe Phe Asn His Lys His Ser Asp Ile Thr Lys Ser Lys Ile Ser Glu 
            180                 185                 190         


Lys Met Lys Gly Lys Lys Pro Ser Asn Ile Lys Lys Ile Ser Cys Asp 
        195                 200                 205             


Gly Val Ile Phe Asp Cys Ala Ala Asp Ala Ala Arg His Phe Lys Ile 
    210                 215                 220                 


Ser Ser Gly Leu Val Thr Tyr Arg Val Lys Ser Asp Lys Trp Asn Trp 
225                 230                 235                 240 


Phe Tyr Ile Asn Ala 
                245 


<210>  2
<211>  168
<212>  PRT
<213>  Bacteriophage T4

<400>  2

Met Lys Ser Gly Ile Tyr Gln Ile Lys Asn Thr Leu Asn Asn Lys Val 
1               5                   10                  15      


Tyr Val Gly Ser Ala Lys Asp Phe Glu Lys Arg Trp Lys Arg His Phe 
            20                  25                  30          


Lys Asp Leu Glu Lys Gly Cys His Ser Ser Ile Lys Leu Gln Arg Ser 
        35                  40                  45              


Phe Asn Lys His Gly Asn Val Phe Glu Cys Ser Ile Leu Glu Glu Ile 
    50                  55                  60                  


Pro Tyr Glu Lys Asp Leu Ile Ile Glu Arg Glu Asn Phe Trp Ile Lys 
65                  70                  75                  80  


Glu Leu Asn Ser Lys Ile Asn Gly Tyr Asn Ile Ala Asp Ala Thr Phe 
                85                  90                  95      


Gly Asp Thr Cys Ser Thr His Pro Leu Lys Glu Glu Ile Ile Lys Lys 
            100                 105                 110         


Arg Ser Glu Thr Val Lys Ala Lys Met Leu Lys Leu Gly Pro Asp Gly 
        115                 120                 125             


Arg Lys Ala Leu Tyr Ser Lys Pro Gly Ser Lys Asn Gly Arg Trp Asn 
    130                 135                 140                 


Pro Glu Thr His Lys Phe Cys Lys Cys Gly Val Arg Ile Gln Thr Ser 
145                 150                 155                 160 


Ala Tyr Thr Cys Ser Lys Cys Arg 
                165             


<210>  3
<211>  510
<212>  DNA
<213>  Bacteriophage T4

<400>  3
atgaaaagcg gaatttatca gattaaaaat actttaaaca ataaagtata tgtaggaagt       60

gctaaagatt ttgaaaagag atggaagagg cattttaaag atttagaaaa aggatgccat      120

tcttctataa aacttcagag gtcttttaac aaacatggta atgtgtttga atgttctatt      180

ttggaagaaa ttccatatga gaaagatttg attattgaac gagaaaattt ttggattaaa      240

gagcttaatt ctaaaattaa tggatacaat attgctgatg caacgtttgg tgatacatgt      300

tctacgcatc cattaaaaga agaaattatt aagaaacgtt ctgaaactgt taaagctaag      360

atgcttaaac ttggacctga tggtcggaaa gctctttaca gtaaacccgg aagtaaaaac      420

gggcgttgga atccagaaac ccataagttt tgtaagtgcg gtgttcgcat acaaacttct      480

gcttatactt gtagtaaatg cagaggatcc                                       510


<210>  4
<211>  37
<212>  DNA
<213>  Bacteriophage T4

<400>  4
ccatggtacc agatctatga aaagcggaat ttatcag                                37


<210>  5
<211>  31
<212>  DNA
<213>  Bacteriophage T4

<400>  5
ccggatcctc tgcatttact acaagtataa g                                      31


<210>  6
<211>  4858
<212>  DNA
<213>  Artificial sequence

<220>
<223>  fusion of I-TevI from Bacteriophage T4 and AvrXa7 from 
       Xanthomonas oryzae

<400>  6
atgaaaagcg gaatttatca gattaaaaat actttaaaca ataaagtata tgtaggaagt       60

gctaaagatt ttgaaaagag atggaagagg cattttaaag atttagaaaa aggatgccat      120

tcttctataa aacttcagag gtcttttaac aaacatggta atgtgtttga atgttctatt      180

ttggaagaaa ttccatatga gaaagatttg attattgaac gagaaaattt ttggattaaa      240

gagcttaatt ctaaaattaa tggatacaat attgctgatg caacgtttgg tgatacatgt      300

tctacgcatc cattaaaaga agaaattatt aagaaacgtt ctgaaactgt taaagctaag      360

atgcttaaac ttggacctga tggtcggaaa gctctttaca gtaaacccgg aagtaaaaac      420

gggcgttgga atccagaaac ccataagttt tgtaagtgcg gtgttcgcat acaaacttct      480

gcttatactt gtagtaaatg cagaggatct gccaccatgg atcccattcg ttcgcgcacg      540

ccaagtcctg cccgcgagct tctgcccgga ccccaaccgg atagggttca gccgactgca      600

gatcgggggg gggctccgcc tgctggcggc cccctggatg gcttgcccgc tcggcggacg      660

atgtcccgga cccggctgcc atctccccct gcgccctcgc ctgcgttctc ggcgggcagc      720

ttcagcgatc tgctccgtca gttcgatccg tcgcttcttg atacatcgct tcttgattcg      780

atgcctgccg tcggcacgcc gcatacagcg gctgccccag cagagtggga tgaggtgcaa      840

tcgggtctgc gtgcagccga tgacccgcca cccaccgtgc gtgtcgctgt cactgccgcg      900

cggccgccgc gcgccaagcc ggccccgcga cggcgtgcgg cgcaaccctc cgacgcttcg      960

ccggccgcgc aggtggatct acgcacgctc ggctacagtc agcagcagca agagaagatc     1020

aaaccgaagg tgcgttcgac agtggcgcag caccacgagg cactggtggg ccatgggttt     1080

acacacgcgc acatcgttgc gctcagccaa cacccggcag cgttagggac cgtcgctgtc     1140

aagtatcagc acataatcac ggcgttgcca gaggcgacac acgaagacat cgttggcgtc     1200

ggcaaacagt ggtccggcgc acgcgccctg gaggccttgc tcacgaaggc gggggagttg     1260

agaggtccgc cgttacagtt ggacacaggc caacttctca agattgcaaa acgtggcggc     1320

gtgaccgcag tggaggcagt gcatgcatgg cgcaatgcac tgacgggtgc ccccctgaac     1380

ctgaccccgg accaagtggt ggccatcgcc agcaatattg gcggcaagca ggcgctggag     1440

acggtacagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaggtc     1500

gtggccatcg ccagccatgg cggcggcaag caggcgctgg agacggtgca gcggctgttg     1560

ccggtgctgt gccaggacca tggcctgacc ccggaccagg tggtggccat cgccagcaat     1620

attggcggca agcaggcgct agagacggtg cagcggctgt tgccggtgct gtgccaggcc     1680

catggcctga ccccggacca ggtcgtggcc atcgccagca atattggcgg caagcaggcg     1740

ctggagacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggcc     1800

caggtggtgg ccatcgccag caatagtggc ggcaagcagg cgctggagac ggtgcagcgg     1860

ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtcgt ggccatcgcc     1920

agccacgatg gcggcaagca ggcgctggag acgctgcagc ggctgttgcc ggtgctgtgc     1980

caggaccatg gcctgacccc ggaccaggtc gtggccatcg ccaacaataa cggcggcaag     2040

caggcgctgg agacgctgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc     2100

ccggaccaag tggtggccat cgccagccac gatggcggca agcaggcgct ggagacggtg     2160

cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca ggtggtggcc     2220

atcgccagcc acgatggcgg caagcaggcg ctggagacgg tgcagcggct gttgccggtg     2280

ctgtgccagg accatggcct gaccccggcc caagtggtgg ccatcgccag ccacgatggc     2340

ggcaagcagg cgctggagac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc     2400

ctgaccccgg accaggtggt ggccatcgcc agcaatagcg gcggcaagca ggcgctggag     2460

acggtacagc ggctgttgcc ggtgctgtgc caggaccatg gactgacccc ggaccaggtc     2520

gtggccatcg ccagcaatgg cggcaagcag gcgctggaga cggtacagcg gctgttgccg     2580

gtgctgtgcc aggaccatgg cctgaccccg gaccaggtcg tggccatcgc cagcaatggc     2640

ggcaagcagg cgctggagac ggtgcagcgg ctgttgccgg tacagcggct gttgccggtg     2700

ctgtgccagg accatggcct gacccaggac caggtggtgg ccatcgccag ccacgatggc     2760

ggcaagcagg cgctggagac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc     2820

ctgaccccgg accaagtggt ggccatcgcc agccacgatg gcggcaaaca ggcgctggag     2880

acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaggtg     2940

gtggccatcg ccagcaatag tggcggcaag caggcgctgg agacggtgca gcggctgttg     3000

ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggccat cgccagcaat     3060

agtggcggca agcaggcgct ggagacggtg cagcggctgt tgccggtgct gtgccaggac     3120

catggcctga ccccggacca ggtggtggcc atcgccagca ataacggcgg caagcaggcg     3180

ctggagacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac     3240

caggtcgtgg ccatcgccaa caataacggc ggcaagcagg cgctggagac ggtgcagcgg     3300

ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg cgcaggtggt ggccatcgcc     3360

agcaatattg gcggcaagca ggcgctggag acggtgcagc ggctgttgcc ggtgctgtgc     3420

caggaccatg gcctgaccct ggaccaggtg gtggccattg ccagcaatgg cggcagcaaa     3480

caggcgctag agacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc     3540

ccggaccaag tggtggccat cgccaacaat aacggcggca agcaggcgct ggagacggtg     3600

cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca ggtcgtggcc     3660

atcgccagca atattggcgg caagcaggcg ctggagacgg tgcagcggct gttgccggtg     3720

ctgtgccagg accatggcct gaccctggac caggtggtgg ccatcgccag caatggcggc     3780

aagcaggcgc tggagacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg     3840

accccgaacc aggtggtggc catcgccagc aatagtggcg gcaagcaggc gctggagacg     3900

cctgaccccg aaccaggtgg tggccatcgc cagcaatggc ggcaagcagg cgctggagag     3960

cattgttgcc cagttatctc gccctgatcc ggcgttggcc gcgttgacca acgaccacct     4020

cgtcgccttg gcctgcctcg gcggacgtcc tgccctggat gcagtgaaaa agggattgcc     4080

gcacgcgccg gaattgatca gaagaatcaa tcgccgcatt cccgaacgca cgtcccatcg     4140

cgttcccgac ctcgcgcacg tggttcgcgt gcttggtttt ttccagagcc actcccaccc     4200

agcgcaagca ttcgatgacg ccatgacgca gttcgagatg agcaggcacg gcttggtaca     4260

gctctttcgc agagtgggcg tcaccgaatt cgaagcccgc tacggaacgc tccccccagc     4320

ctcgcagcgt tgggaccgta tcctccaggc atcagggatg aaaagggcca aaccgtcccc     4380

tacttcagct caaacaccgg atcaggcgtc tttgcatgca gattacaagg acgacgacga     4440

caagaaggat tacaaggacg acgacgacaa gaagggtcga cccagcccaa tgcacgaggg     4500

agatcagacg cgggcaagca gccgtaaacg gtcccgatcg gatcgtgctg tcaccggccc     4560

ctccacacag caatctttcg aggtgcgcgt tcccgaacag caagatgcgc tgcatttgcc     4620

cctcagctgg agggtaaaac gcccgcgtac caggatcggg ggcggcctcc cggatcctgg     4680

tacgcccatc gctgccgacc tggcagcgtc cagcaccgtg atgtgggaac aagatgcggc     4740

ccccttcgca ggggcagcgg atgatttccc ggcattcaac gaagaggagc tcgcatggtt     4800

gatggagcta ttgcctcagt caggctcagt cggagggacg atcaagcttc tagactag       4858


<210>  7
<211>  27
<212>  DNA
<213>  Xanthomonas oryzae

<400>  7
tataaacccc ctccaaccag gtgctaa                                           27


<210>  8
<211>  4173
<212>  DNA
<213>  artifical sequence

<400>  8
atgaaaagcg gaatttatca gattaaaaat actttaaaca ataaagtata tgtaggaagt       60

gctaaagatt ttgaaaagag atggaagagg cattttaaag atttagaaaa aggatgccat      120

tcttctataa aacttcagag gtcttttaac aaacatggta atgtgtttga atgttctatt      180

ttggaagaaa ttccatatga gaaagatttg attattgaac gagaaaattt ttggattaaa      240

gagcttaatt ctaaaattaa tggatacaat attgctgatg caacgtttgg tgatacatgt      300

tctacgcatc cattaaaaga agaaattatt aagaaacgtt ctgaaactgt taaagctaag      360

atgcttaaac ttggacctga tggtcggaaa gctctttaca gtaaacccgg aagtaaaaac      420

gggcgttgga atccagaaac ccataagttt tgtaagtgcg gtgttcgcat acaaacttct      480

gcttatactt gtagtaaatg cagaggatct gccaccatgg atcccattcg ttcgcgcacg      540

ccaagtcctg cccgcgagct tctgcccgga ccccaaccgg atagggttca gccgactgca      600

gatcgggggg gggctccgcc tgctggcggc cccctggatg gcttgcccgc tcggcggacg      660

atgtcccgga cccggctgcc atctccccct gcgccctcgc ctgcgttctc ggcgggcagc      720

ttcagcgatc tgctccgtca gttcgatccg tcgcttcttg atacatcgct tcttgattcg      780

atgcctgccg tcggcacgcc gcatacagcg gctgccccag cagagtggga tgaggtgcaa      840

tcgggtctgc gtgcagccga tgacccgcca cccaccgtgc gtgtcgctgt cactgccgcg      900

cggccgccgc gcgccaagcc ggccccgcga cggcgtgcgg cgcaaccctc cgacgcttcg      960

ccggccgcgc aggtggatct acgcacgctc ggctacagtc agcagcagca agagaagatc     1020

aaaccgaagg tgcgttcgac agtggcgcag caccacgagg cactggtggg ccatgggttt     1080

acacacgcgc acatcgttgc gctcagccaa cacccggcag cgttagggac cgtcgctgtc     1140

aagtatcagc acataatcac ggcgttgcca gaggcgacac acgaagacat cgttggcgtc     1200

ggcaaacagt ggtccggcgc acgcgccctg gaggccttgc tcacgaaggc gggggagttg     1260

agaggtccgc cgttacagtt ggacacaggc caacttctca agattgcaaa acgtggcggc     1320

gtgaccgcag tggaggcagt gcatgcatgg cgcaatgcac tgacgggtgc ccccctgaac     1380

ctgaccccgg accaagtggt ggccatcgcc agcaatattg gcggcaagca ggcgctggag     1440

acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaggtc     1500

gtggccatcg ccagcaatgg cggcggcaag caggccttgg agacggtgca gcggctgttg     1560

ccggtgctgt gccaggccca tggcctgacc ccggcccaag tggtggccat cgccagcaat     1620

ggcggcggca agcaggctct ggagacggta cagcggctgt tgccggtgct gtgccaggac     1680

catggcctga ccccggacca ggtcgtggcc atcgccagca atattggcgg caagcaggcg     1740

ttggagacgg tacagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac     1800

caggtggtgg ccatcgccag caatattggc ggcaagcagg ctttggagac ggtacagcgg     1860

ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaggtggt ggccatcgcc     1920

agcaatattg gcggcaagca ggcattggag acggtacagc ggctgttgcc ggtgctgtgc     1980

caggaccatg gcctgacccc ggaccaggtc gtggccatcg ccagcaatgg cggcggcaag     2040

caggccctgg agacggtaca gcggctgttg ccggtgctgt gccaggacca tggcctgacc     2100

ccggaccagg tggtggccat cgccagcaat attggcggca agcaggcact ggagacggta     2160

cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca ggtggtggcc     2220

atcgccaaca ataacggcgg caagcaggca ctggaaacac tgcagcggct gttgccggtg     2280

ctgtgccagg accatggcct gacccaggac caggtggtgg ccatcgccag ccacgatggc     2340

ggcaagcagg ccttggagac ggtgcagcgg ctgttgccgg tgctgtgcca ggcccatggc     2400

ctgaccccgg cccaagtggt ggccatcgcc agcaatggcg gcggcaagca ggctctggag     2460

acggtacagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaggtc     2520

gtggccatcg ccagcaatgg cggcggcaag caggcgttgg agacggtaca gcggctgttg     2580

ccggtgctgt gccaggacca tggcctgacc ccggaccagg tggtggccat cgccaacaat     2640

aacggcggca agcaggcttt ggagacggta cagcggctgt tgccggtgct gtgccaggac     2700

catggcctga ccccggacca ggtcgtggcc atcgccaaca ataacggcgg caagcaggca     2760

ttggagacgg tacagcggct gttgccggtg ctgtgccagg accatggcct gacccaggac     2820

caggtggtgg ccatcgccag ccacgatggc ggcaagcagg ccctggagac ggtacagcgg     2880

ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaggtggt ggccatcgcc     2940

agcaatattg gcggcaagca ggcactggag acggtacagc ggctgttgcc ggtgctgtgc     3000

caggaccatg gcctgacccc ggaccaggtc gtggccatcg ccaacaataa cggcggcaag     3060

caggcactgg agactgtaca gcggctgttg ccggtgctgt gccaggacca tggcctgacc     3120

caggaccagg tggtggccat cgccagccac gatggcggca agcaggcact ggagacggta     3180

cagcggctgt tgccggtgct gtgccaggac catggcctga ccccgaacca ggtggtggcc     3240

atcgccagca atggcggcaa gcaggcgctg gagagcattg ttgcccagtt atctcgccct     3300

gatccggcgt tggccgcgtt gaccaacgac cacctcgtcg ccttggcctg cctcggcgga     3360

cgtcctgccc tggatgcagt gaaaaaggga ttgccgcacg cgccggaatt gatcagaaga     3420

atcaatcgcc gcattcccga acgcacgtcc catcgcgttc ccgacctcgc gcacgtggtt     3480

cgcgtgcttg gttttttcca gagccactcc cacccagcgc aagcattcga tgacgccatg     3540

acgcagttcg agatgagcag gcacggcttg gtacagctct ttcgcagagt gggcgtcacc     3600

gaattcgaag cccgctacgg aacgctcccc ccagcctcgc agcgttggga ccgtatcctc     3660

caggcatcag ggatgaaaag ggccaaaccg tcccctactt cagctcaaac accggatcag     3720

gcgtctttgc atgcagatta caaggacgac gacgacaaga aggattacaa ggacgacgac     3780

gacaagaagg gtcgacccag cccaatgcac gagggagatc agacgcgggc aagcagccgt     3840

aaacggtccc gatcggatcg tgctgtcacc ggcccctcca cacagcaatc tttcgaggtg     3900

cgcgttcccg aacagcaaga tgcgctgcat ttgcccctca gctggagggt aaaacgcccg     3960

cgtaccagga tcgggggcgg cctcccggat cctggtacgc ccatcgctgc cgacctggca     4020

gcgtccagca ccgtgatgtg ggaacaagat gcggccccct tcgcaggggc agcggatgat     4080

ttcccggcat tcaacgaaga ggagctcgca tggttgatgg agctattgcc tcagtcaggc     4140

tcagtcggag ggacgatcaa gcttctagac tag                                  4173


<210>  9
<211>  19
<212>  DNA
<213>  Xanthomonas oryzae

<400>  9
tattaaatag cttggcagc                                                    19


<210>  10
<211>  3969
<212>  DNA
<213>  Artificial sequence

<220>
<223>  fusion of I-TevI from Bacteriophage T4 and AvrXa7 from 
       Xanthomonas oryzae

<400>  10
atgaaaagcg gaatttatca gattaaaaat actttaaaca ataaagtata tgtaggaagt       60

gctaaagatt ttgaaaagag atggaagagg cattttaaag atttagaaaa aggatgccat      120

tcttctataa aacttcagag gtcttttaac aaacatggta atgtgtttga atgttctatt      180

ttggaagaaa ttccatatga gaaagatttg attattgaac gagaaaattt ttggattaaa      240

gagcttaatt ctaaaattaa tggatacaat attgctgatg caacgtttgg tgatacatgt      300

tctacgcatc cattaaaaga agaaattatt aagaaacgtt ctgaaactgt taaagctaag      360

atgcttaaac ttggacctga tggtcggaaa gctctttaca gtaaacccgg aagtaaaaac      420

gggcgttgga atccagaaac ccataagttt tgtaagtgcg gtgttcgcat acaaacttct      480

gcttatactt gtagtaaatg cagaggatct gccaccatgg atcccattcg ttcgcgcacg      540

ccaagtcctg cccgcgagct tctgcccgga ccccaaccgg atagggttca gccgactgca      600

gatcgggggg gggctccgcc tgctggcggc cccctggatg gcttgcccgc tcggcggacg      660

atgtcccgga cccggctgcc atctccccct gcgccctcgc ctgcgttctc ggcgggcagc      720

ttcagcgatc tgctccgtca gttcgatccg tcgcttcttg atacatcgct tcttgattcg      780

atgcctgccg tcggcacgcc gcatacagcg gctgccccag cagagtggga tgaggtgcaa      840

tcgggtctgc gtgcagccga tgacccgcca cccaccgtgc gtgtcgctgt cactgccgcg      900

cggccgccgc gcgccaagcc ggccccgcga cggcgtgcgg cgcaaccctc cgacgcttcg      960

ccggccgcgc aggtggatct acgcacgctc ggctacagtc agcagcagca agagaagatc     1020

aaaccgaagg tgcgttcgac agtggcgcag caccacgagg cactggtggg ccatgggttt     1080

acacacgcgc acatcgttgc gctcagccaa cacccggcag cgttagggac cgtcgctgtc     1140

aagtatcagc acataatcac ggcgttgcca gaggcgacac acgaagacat cgttggcgtc     1200

ggcaaacagt ggtccggcgc acgcgccctg gaggccttgc tcacgaaggc gggggagttg     1260

agaggtccgc cgttacagtt ggacacaggc caacttctca agattgcaaa acgtggcggc     1320

gtgaccgcag tggaggcagt gcatgcatgg cgcaatgcac tgacgggtgc ccccctgaac     1380

ctgaccccgg accaagtggt ggccatcgcc agcaatattg gcggcaagca ggcgctggag     1440

acggtacagc ggctgttgcc ggtgctgtgc caggaccatg gcctgaccca ggaacaggtg     1500

gtggccatcg ccagccacga tggcggcaag caggctttgg agacggtaca gcggctgttg     1560

ccggtgctgt gccaggcaca tggcctgacc ccggcccaag tggtggccat cgccagcaat     1620

ggcggcggca agcaggctct ggagacggta cagcggctgt tgccggtgct gtgccaggac     1680

catggcctga ccccggacca ggtggtggcc atcgccaaca ataacggcgg caagcaggcg     1740

ttggagacgg tacagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac     1800

caggtcgtgg ccatcgccag caatggcggc ggcaagcagg ctttggagac ggtacagcgg     1860

ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaggtcgt ggccatcgcc     1920

agcaatggcg gcggcaagca ggcattggag acggtacagc ggctgttgcc ggtgctgtgc     1980

caggaccatg gcctgacccc ggaccaggtc gtggccatcg ccaacaataa cggcggcaag     2040

caggccctgg agacggtaca gcggctgttg ccggtgctgt gccaggacca tggcctgacc     2100

ccggaccagg tggtggccat cgccagcaat attggcggca agcaggcact ggagacggta     2160

cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca ggtggtggcc     2220

atcgccagcc acgatggcgg caagcaggca ctggaaacac tgcagcggct gttgccggtg     2280

ctgtgccagg accatggcct gaccccggac caggtggtgg ccatcgccag ccacgatggc     2340

ggcaagcagg ccttggagac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc     2400

ctgaccccgg accaggtggt ggccatcgcc agccacgatg gcggcaagca ggctctggag     2460

acggtacagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaggtc     2520

gtggccatcg ccagcaatat tggcggcaag caggcgttgg agacggtaca gcggctgttg     2580

ccggtgctgt gccaggacca tggcctgacc ccggaccagg tcgtggccat cgccagcaat     2640

attggcggca agcaggcttt ggagacggta cagcggctgt tgccggtgct gtgccaggac     2700

catggcctga ccccggacca ggtcgtggcc atcgccagca atggcggcgg caagcaggca     2760

ttggagacgg tacagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac     2820

caggtggtgg ccatcgccaa caataacggc ggcaagcagg ccctggagac ggtacagcgg     2880

ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaggtggt ggccatcgcc     2940

agccacgatg gcggcaagca ggcactggag acggtacagc ggctgttgcc ggtgctgtgc     3000

caggaccatg gcctgacccc gaaccaggtg gtggccatcg ccagcaatgg cggcaagcag     3060

gcgctggaga gcattgttgc ccagttatct cgccctgatc cggcgttggc cgcgttgacc     3120

aacgaccacc tcgtcgcctt ggcctgcctc ggcggacgtc ctgccctgga tgcagtgaaa     3180

aagggattgc cgcacgcgcc ggaattgatc agaagaatca atcgccgcat tcccgaacgc     3240

acgtcccatc gcgttcccga cctcgcgcac gtggttcgcg tgcttggttt tttccagagc     3300

cactcccacc cagcgcaagc attcgatgac gccatgacgc agttcgagat gagcaggcac     3360

ggcttggtac agctctttcg cagagtgggc gtcaccgaat tcgaagcccg ctacggaacg     3420

ctccccccag cctcgcagcg ttgggaccgt atcctccagg catcagggat gaaaagggcc     3480

aaaccgtccc ctacttcagc tcaaacaccg gatcaggcgt ctttgcatgc agattacaag     3540

gacgacgacg acaagaagga ttacaaggac gacgacgaca agaagggtcg acccagccca     3600

atgcacgagg gagatcagac gcgggcaagc agccgtaaac ggtcccgatc ggatcgtgct     3660

gtcaccggcc cctccacaca gcaatctttc gaggtgcgcg ttcccgaaca gcaagatgcg     3720

ctgcatttgc ccctcagctg gagggtaaaa cgcccgcgta ccaggatcgg gggcggcctc     3780

ccggatcctg gtacgcccat cgctgccgac ctggcagcgt ccagcaccgt gatgtgggaa     3840

caagatgcgg cccccttcgc aggggcagcg gatgatttcc cggcattcaa cgaagaggag     3900

ctcgcatggt tgatggagct attgcctcag tcaggctcag tcggagggac gatcaagctt     3960

ctagactag                                                             3969


<210>  11
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  fusion of I-TevI from Bacteriophage T4 and AvrXa7 from 
       Xanthomonas oryzae

<400>  11
tactgttgac ccaatgc                                                      17


<210>  12
<211>  811
<212>  DNA
<213>  artifical sequence

<400>  12
atgaaaagcg gaatttatca gattaaaaat actttaaaca ataaagtata tgtaggaagt       60

gctaaagatt ttgaaaagag atggaagagg cattttaaag atttagaaaa aggatgccat      120

tcttctataa aacttcagag gtcttttaac aaacatggta atgtgtttga atgttctatt      180

ttggaagaaa ttccatatga gaaagatttg attattgaac gagaaaattt ttggattaaa      240

gagcttaatt ctaaaattaa tggatacaat attgctgatg caacgtttgg tgatacatgt      300

tctacgcatc cattaaaaga agaaattatt aagaaacgtt ctgaaactgt taaagctaag      360

atgcttaaac ttggacctga tggtcggaaa gctctttaca gtaaacccgg aagtaaaaac      420

gggcgttgga atccagaaac ccataagttt tgtaagtgcg gtgttcgcat acaaacttct      480

gcttatactt gtagtaaatg cagaggatcc ggaatcacta gtagagaagc cctacgcttg      540

cccagtggag tcctgtgatc gccgcttctc ccgctccgac gagctcaccc gccacatccg      600

catccacaca ggccagaagc ccttccagtg ccgcatctgc atgcgcaact tcagccgcag      660

cgaccacctc accacccaca tccgcaccca cacaggcgaa aagccctttg cctgcgacat      720

ctgtggaaga aagtttgcca ggagcgatga acgcaagagg cataccaaga tccacggatc      780

cggaatcact agtctcgagt catgtaatta g                                     811


<210>  13
<211>  92
<212>  PRT
<213>  Bacteriophage T4

<400>  13

Met Lys Ser Gly Ile Tyr Gln Ile Lys Asn Thr Leu Asn Asn Lys Val 
1               5                   10                  15      


Tyr Val Gly Ser Ala Lys Asp Phe Glu Lys Arg Trp Lys Arg His Phe 
            20                  25                  30          


Lys Asp Leu Glu Lys Gly Cys His Ser Ser Ile Lys Leu Gln Arg Ser 
        35                  40                  45              


Phe Asn Lys His Gly Asn Val Phe Glu Cys Ser Ile Leu Glu Glu Ile 
    50                  55                  60                  


Pro Tyr Glu Lys Asp Leu Ile Ile Glu Arg Glu Asn Phe Trp Ile Lys 
65                  70                  75                  80  


Glu Leu Asn Ser Lys Ile Asn Gly Tyr Asn Ile Ala 
                85                  90          


<210>  14
<211>  22
<212>  PRT
<213>  Bacteriophage T4

<400>  14

Asp Ala Thr Phe Gly Asp Thr Cys Ser Thr His Pro Leu Lys Glu Glu 
1               5                   10                  15      


Ile Ile Lys Lys Arg Ser 
            20          


<210>  15
<211>  36
<212>  PRT
<213>  Bacteriophage T4

<400>  15

Glu Thr Val Lys Ala Lys Met Leu Lys Leu Gly Pro Asp Gly Arg Lys 
1               5                   10                  15      


Ala Leu Tyr Ser Lys Pro Gly Ser Lys Asn Gly Arg Trp Asn Pro Glu 
            20                  25                  30          


Thr His Lys Phe 
        35      


<210>  16
<211>  18
<212>  PRT
<213>  Bacteriophage T4

<400>  16

Cys Lys Cys Gly Val Arg Ile Gln Thr Ser Ala Tyr Thr Cys Ser Lys 
1               5                   10                  15      


Cys Arg 
        


<210>  17
<211>  77
<212>  PRT
<213>  Bacteriophage T4

<400>  17

Asn Arg Ser Gly Glu Asn Asn Ser Phe Phe Asn His Lys His Ser Asp 
1               5                   10                  15      


Ile Thr Lys Ser Lys Ile Ser Glu Lys Met Lys Gly Lys Lys Pro Ser 
            20                  25                  30          


Asn Ile Lys Lys Ile Ser Cys Asp Gly Val Ile Phe Asp Cys Ala Ala 
        35                  40                  45              


Asp Ala Ala Arg His Phe Lys Ile Ser Ser Gly Leu Val Thr Tyr Arg 
    50                  55                  60                  


Val Lys Ser Asp Lys Trp Asn Trp Phe Tyr Ile Asn Ala 
65                  70                  75          


<210>  18
<211>  313
<212>  DNA
<213>  Artificial sequence

<220>
<223>  fustion protein edited sequence

<400>  18
atgcatctcc ccctactgta caccaccaaa agtgaattca tgagcttagc acctggttgg       60

agggggttta tatcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt      120

gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat      180

gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta      240

ctaaaaacac atgtggatat cttgactgat ttttccatat ggagggcaca gttaagccgc      300

taaaggcatt atc                                                         313


<210>  19
<211>  873
<212>  DNA
<213>  artificial sequence

<220>
<223>  fusion sequence

<400>  19
atgcatctcc ccctactgta caccaccaaa agtgaattca tgagcttagc acctggttgg       60

agggggttta tatcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt      120

gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat      180

gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta      240

ctaaaaacac atgtggatat cttgactgat ttttccatgg agggcacagt taagccgcta      300

aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt tgctgacatt      360

ggtaatacag tcaaattgca gtactctgcg ggtgtataca gaatagcaga atgggcagac      420

attacgaatg cacacggtgt ggtgggccca ggtattgtta gcggtttgaa gcaggcggcg      480

gaagaagtaa caaaggaacc tagaggcctt ttgatgttag cagaattgtc atgcaagggc      540

tccctagcta ctggagaata tactaagggt actgttgaca ttgcgaagag cgacaaagat      600

tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg ttacgattgg      660

ttgattatga cacccggtgt gggtttagat gacaagggag acgcattggg tcaacagtat      720

agaaccgtgg atgatgtggt ctctacagga tctgacatta ttattgttgg aagaggacta      780

tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa      840

gcatatttga gaagatgcgg ccagcaaaac taa                                   873


<210>  20
<211>  469
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNA edited sequence

<400>  20
agtaacaaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc       60

tagctactgg agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg      120

ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga      180

ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtagtaac      240

aaaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctagct      300

actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc      360

ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg      420

acacccggtg tgggtttaga tgacaaggga gacgcattgg gtcaacagt                  469


<210>  21
<211>  873
<212>  DNA
<213>  Artificial sequence

<220>
<223>  URA3 functional sequence

<400>  21
atgcatctcc ccctactgta caccaccaaa agtgaattca tgagcttagc acctggttgg       60

agggggttta tatcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt      120

gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat      180

gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta      240

ctaaaaacac atgtggatat cttgactgat ttttccatgg agggcacagt taagccgcta      300

aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt tgctgacatt      360

ggtaatacag tcaaattgca gtactctgcg ggtgtataca gaatagcaga atgggcagac      420

attacgaatg cacacggtgt ggtgggccca ggtattgtta gcggtttgaa gcaggcggcg      480

gaagaagtaa caaaggaacc tagaggcctt ttgatgttag cagaattgtc atgcaagggc      540

tccctagcta ctggagaata tactaagggt actgttgaca ttgcgaagag cgacaaagat      600

tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg ttacgattgg      660

ttgattatga cacccggtgt gggtttagat gacaagggag acgcattggg tcaacagtat      720

agaaccgtgg atgatgtggt ctctacagga tctgacatta ttattgttgg aagaggacta      780

tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa      840

gcatatttga gaagatgcgg ccagcaaaac taa                                   873


