                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  POLYPEPTIDES HAVING PROTEASE ACTIVITY AND POLYNUCLEOTIDES 
       ENCODING SAME

<130>  12132-WO-PCT

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  1505
<212>  DNA
<213>  Saccharothrix australiensis


<220>
<221>  CDS
<222>  (101)..(1405)

<220>
<221>  sig_peptide
<222>  (101)..(187)

<400>  1
agttatgact cgactggcat atccgccacc tcgctcagcc ggctagcgtc gtccgcacgg       60

ccccgcatcc cgtgtcacca cccctgcaag gagacaacgg atg act cga cgc atc        115
                                            Met Thr Arg Arg Ile           
                                            1               5             

gcc gcc gcc gtc gcc gtg gcg gtc atg agc gct gcc ggt gtg gca gcc        163
Ala Ala Ala Val Ala Val Ala Val Met Ser Ala Ala Gly Val Ala Ala           
                10                  15                  20                

gct ctg acc acc gcc gcg aac gcc ggt cca ccg aca acg cac cag gag        211
Ala Leu Thr Thr Ala Ala Asn Ala Gly Pro Pro Thr Thr His Gln Glu           
            25                  30                  35                    

gag agc ggc ctc atc gcc gcg atg gcg cgc gac ttc aag atc acg ccc        259
Glu Ser Gly Leu Ile Ala Ala Met Ala Arg Asp Phe Lys Ile Thr Pro           
        40                  45                  50                        

gac cag gcg cgc gcc cgg ctc gtc cgg gag gcc aag gcc gcg acc acc        307
Asp Gln Ala Arg Ala Arg Leu Val Arg Glu Ala Lys Ala Ala Thr Thr           
    55                  60                  65                            

gag cag agc ctg aag tcg cgg ctc ggc ggc cac tac gcg ggc gcc tgg        355
Glu Gln Ser Leu Lys Ser Arg Leu Gly Gly His Tyr Ala Gly Ala Trp           
70                  75                  80                  85            

ctg aac gag ggc gcc acc gaa ctc gtc gtc gcc gtc acc gac gcg gcg        403
Leu Asn Glu Gly Ala Thr Glu Leu Val Val Ala Val Thr Asp Ala Ala           
                90                  95                  100               

cag gcc aag gtg gtc gag gac gcc ggc gcg acg ccg aag gtc gtg cag        451
Gln Ala Lys Val Val Glu Asp Ala Gly Ala Thr Pro Lys Val Val Gln           
            105                 110                 115                   

cgc agc cag atc cag ctc gac gag ctg aag gcc aag ctg gac gcg aac        499
Arg Ser Gln Ile Gln Leu Asp Glu Leu Lys Ala Lys Leu Asp Ala Asn           
        120                 125                 130                       

aag aac gcg ccg aag gac gtg ccc gcg tgg tac gtc gac gtg aag acc        547
Lys Asn Ala Pro Lys Asp Val Pro Ala Trp Tyr Val Asp Val Lys Thr           
    135                 140                 145                           

aac tcc gtg gtc gtg ctg gcc cgc aac acc gcg agc gcc aag gcg ttc        595
Asn Ser Val Val Val Leu Ala Arg Asn Thr Ala Ser Ala Lys Ala Phe           
150                 155                 160                 165           

gcc cgc gcc agc ggt ctg agc gag gcg gac gtc cgg atc gag cag tcc        643
Ala Arg Ala Ser Gly Leu Ser Glu Ala Asp Val Arg Ile Glu Gln Ser           
                170                 175                 180               

acc gag gac ccg cgc ccg ctg atc gac gtc atc ggc ggc aac gcg tac        691
Thr Glu Asp Pro Arg Pro Leu Ile Asp Val Ile Gly Gly Asn Ala Tyr           
            185                 190                 195                   

tac atg ggc agc ggc ggt cgc tgc tcg gtc ggc ttc tcg gtc aac ggc        739
Tyr Met Gly Ser Gly Gly Arg Cys Ser Val Gly Phe Ser Val Asn Gly           
        200                 205                 210                       

ggc ttc gtg acg gcg ggc cac tgc ggc cgg gtc ggc acc acg acc acc        787
Gly Phe Val Thr Ala Gly His Cys Gly Arg Val Gly Thr Thr Thr Thr           
    215                 220                 225                           

cag ccc agc ggc acg ttc gcc ggt tcc acc ttc ccc ggc cgg gac tac        835
Gln Pro Ser Gly Thr Phe Ala Gly Ser Thr Phe Pro Gly Arg Asp Tyr           
230                 235                 240                 245           

gcc tgg gtc cgc gtg agc tcg ggc aac acc atg cgc ggc ctg gtc aac        883
Ala Trp Val Arg Val Ser Ser Gly Asn Thr Met Arg Gly Leu Val Asn           
                250                 255                 260               

cgc tac ccc ggc acc gtg ccg gtg aag ggc tcc aac gag tcg tcc gtc        931
Arg Tyr Pro Gly Thr Val Pro Val Lys Gly Ser Asn Glu Ser Ser Val           
            265                 270                 275                   

ggc gcg tcg gtc tgc cgt tcc ggc tcg acg acg ggt tgg cac tgc ggc        979
Gly Ala Ser Val Cys Arg Ser Gly Ser Thr Thr Gly Trp His Cys Gly           
        280                 285                 290                       

acc atc cag cag aag aac acc tcc gtg acg tac ccc gag ggc acc atc       1027
Thr Ile Gln Gln Lys Asn Thr Ser Val Thr Tyr Pro Glu Gly Thr Ile           
    295                 300                 305                           

tcc ggt gtg acc cgg acc aac gcc tgc gcc gag ccc ggc gac tcg ggc       1075
Ser Gly Val Thr Arg Thr Asn Ala Cys Ala Glu Pro Gly Asp Ser Gly           
310                 315                 320                 325           

ggt tcg tgg ctc acc ggc gac cag gcc cag ggc gtc acc tcc ggt ggt       1123
Gly Ser Trp Leu Thr Gly Asp Gln Ala Gln Gly Val Thr Ser Gly Gly           
                330                 335                 340               

tcc ggg aac tgc tca tcc ggc ggc acg acg tac ttc cag ccg gtg aac       1171
Ser Gly Asn Cys Ser Ser Gly Gly Thr Thr Tyr Phe Gln Pro Val Asn           
            345                 350                 355                   

ccg atc ctc cag gcg tac ggc ctc cag ctg gtc atc gag ggc ggc ccg       1219
Pro Ile Leu Gln Ala Tyr Gly Leu Gln Leu Val Ile Glu Gly Gly Pro           
        360                 365                 370                       

acc ggc acc acc gga ccg acc acg acg tcc agc aac ccg ggc ggc acg       1267
Thr Gly Thr Thr Gly Pro Thr Thr Thr Ser Ser Asn Pro Gly Gly Thr           
    375                 380                 385                           

acc tgg cag cca ggc gtc gcg tac acg gcc ggt acc acc gtc acg tac       1315
Thr Trp Gln Pro Gly Val Ala Tyr Thr Ala Gly Thr Thr Val Thr Tyr           
390                 395                 400                 405           

gag ggc gtc ggg tac gag tgc ttg cag ggg cac acg tcc caa atc ggc       1363
Glu Gly Val Gly Tyr Glu Cys Leu Gln Gly His Thr Ser Gln Ile Gly           
                410                 415                 420               

tgg gag ccg tcc gcg gtg ccc gct ctg tgg gag cgc gtg ggc               1405
Trp Glu Pro Ser Ala Val Pro Ala Leu Trp Glu Arg Val Gly                   
            425                 430                 435                   

tagcggcaag cttcgcgcgc gggggcgggg cccgactcgc gtcgggcccc ccttccccac     1465

gcgcctaccg gggatgacgg ggcgtttgcg gaggcgcccc                           1505


<210>  2
<211>  435
<212>  PRT
<213>  Saccharothrix australiensis

<400>  2

Met Thr Arg Arg Ile Ala Ala Ala Val Ala Val Ala Val Met Ser Ala 
1               5                   10                  15      


Ala Gly Val Ala Ala Ala Leu Thr Thr Ala Ala Asn Ala Gly Pro Pro 
            20                  25                  30          


Thr Thr His Gln Glu Glu Ser Gly Leu Ile Ala Ala Met Ala Arg Asp 
        35                  40                  45              


Phe Lys Ile Thr Pro Asp Gln Ala Arg Ala Arg Leu Val Arg Glu Ala 
    50                  55                  60                  


Lys Ala Ala Thr Thr Glu Gln Ser Leu Lys Ser Arg Leu Gly Gly His 
65                  70                  75                  80  


Tyr Ala Gly Ala Trp Leu Asn Glu Gly Ala Thr Glu Leu Val Val Ala 
                85                  90                  95      


Val Thr Asp Ala Ala Gln Ala Lys Val Val Glu Asp Ala Gly Ala Thr 
            100                 105                 110         


Pro Lys Val Val Gln Arg Ser Gln Ile Gln Leu Asp Glu Leu Lys Ala 
        115                 120                 125             


Lys Leu Asp Ala Asn Lys Asn Ala Pro Lys Asp Val Pro Ala Trp Tyr 
    130                 135                 140                 


Val Asp Val Lys Thr Asn Ser Val Val Val Leu Ala Arg Asn Thr Ala 
145                 150                 155                 160 


Ser Ala Lys Ala Phe Ala Arg Ala Ser Gly Leu Ser Glu Ala Asp Val 
                165                 170                 175     


Arg Ile Glu Gln Ser Thr Glu Asp Pro Arg Pro Leu Ile Asp Val Ile 
            180                 185                 190         


Gly Gly Asn Ala Tyr Tyr Met Gly Ser Gly Gly Arg Cys Ser Val Gly 
        195                 200                 205             


Phe Ser Val Asn Gly Gly Phe Val Thr Ala Gly His Cys Gly Arg Val 
    210                 215                 220                 


Gly Thr Thr Thr Thr Gln Pro Ser Gly Thr Phe Ala Gly Ser Thr Phe 
225                 230                 235                 240 


Pro Gly Arg Asp Tyr Ala Trp Val Arg Val Ser Ser Gly Asn Thr Met 
                245                 250                 255     


Arg Gly Leu Val Asn Arg Tyr Pro Gly Thr Val Pro Val Lys Gly Ser 
            260                 265                 270         


Asn Glu Ser Ser Val Gly Ala Ser Val Cys Arg Ser Gly Ser Thr Thr 
        275                 280                 285             


Gly Trp His Cys Gly Thr Ile Gln Gln Lys Asn Thr Ser Val Thr Tyr 
    290                 295                 300                 


Pro Glu Gly Thr Ile Ser Gly Val Thr Arg Thr Asn Ala Cys Ala Glu 
305                 310                 315                 320 


Pro Gly Asp Ser Gly Gly Ser Trp Leu Thr Gly Asp Gln Ala Gln Gly 
                325                 330                 335     


Val Thr Ser Gly Gly Ser Gly Asn Cys Ser Ser Gly Gly Thr Thr Tyr 
            340                 345                 350         


Phe Gln Pro Val Asn Pro Ile Leu Gln Ala Tyr Gly Leu Gln Leu Val 
        355                 360                 365             


Ile Glu Gly Gly Pro Thr Gly Thr Thr Gly Pro Thr Thr Thr Ser Ser 
    370                 375                 380                 


Asn Pro Gly Gly Thr Thr Trp Gln Pro Gly Val Ala Tyr Thr Ala Gly 
385                 390                 395                 400 


Thr Thr Val Thr Tyr Glu Gly Val Gly Tyr Glu Cys Leu Gln Gly His 
                405                 410                 415     


Thr Ser Gln Ile Gly Trp Glu Pro Ser Ala Val Pro Ala Leu Trp Glu 
            420                 425                 430         


Arg Val Gly 
        435 


<210>  3
<211>  1305
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic gene

<400>  3
atgacacgtc gcattgccgc tgcagttgca gtagctgtaa tgtctgcggc aggagttgct       60

gcagctctta cgacggctgc taacgctgga cctccaacta cgcatcagga agaatcagga      120

cttatcgctg caatggctcg tgactttaag attacaccgg atcaagcacg tgctcgcctt      180

gtacgtgaag cgaaagcagc aactacagaa caatctttga aatctcgttt gggaggccac      240

tatgctggcg catggcttaa cgaaggtgct actgaattag tagttgctgt tactgatgcc      300

gcacaagcga aagttgttga agatgcggga gctacgccta aggtagtaca acgttcacaa      360

atccaattgg acgagcttaa agcaaaactt gacgctaaca aaaacgctcc aaaagacgta      420

cctgcatggt atgttgatgt aaagacaaat tcagtagtag ttcttgctcg taacacagcg      480

tctgcaaaag catttgctcg cgcatctgga ttatcagaag ctgacgttcg tatcgaacag      540

tctactgaag acccacgtcc gttaatcgac gttattggtg gcaatgcgta ttacatgggt      600

tctggaggcc gttgctcagt aggattttct gtaaatggcg gtttcgttac agctggtcac      660

tgtggtcgtg taggcacaac tactacacaa ccttctggta cttttgctgg ctctactttc      720

ccaggtcgcg actatgcttg ggttcgtgtt tcttctggaa atacgatgcg tggacttgta      780

aatcgttacc ctggtactgt acctgttaaa ggatctaatg aatcatcagt aggcgcttct      840

gtttgtcgtt ctggcagcac gacaggttgg cactgcggaa ctattcagca gaaaaacaca      900

tctgttactt accctgaggg aactatttct ggagtaactc gtacaaatgc gtgcgcagaa      960

cctggtgact ctggtggatc atggcttact ggtgatcagg ctcaaggagt aacatctgga     1020

ggctctggta actgctcttc tggtggcaca acgtatttcc aacctgttaa cccaatctta     1080

caagcatacg gccttcaatt agttattgaa ggtggaccaa ctggtacaac aggtcctacg     1140

acaacatctt ctaaccctgg aggcacaact tggcaacctg gtgttgcata cacggctggc     1200

actactgtta cgtacgaggg cgttggttac gaatgccttc aaggtcacac atctcagatc     1260

ggctgggaac ctagcgcagt tcctgccctt tgggaacgtg ttggc                     1305


<210>  4
<211>  7
<212>  PRT
<213>  Saccharothrix australiensis

<400>  4

Ile Asp Val Ile Gly Gly Asn 
1               5           


<210>  5
<211>  186
<212>  PRT
<213>  Saccharothrix australiensis

<400>  5

Ile Asp Val Ile Gly Gly Asn Ala Tyr Tyr Met Gly Ser Gly Gly Arg 
1               5                   10                  15      


Cys Ser Val Gly Phe Ser Val Asn Gly Gly Phe Val Thr Ala Gly His 
            20                  25                  30          


Cys Gly Arg Val Gly Thr Thr Thr Thr Gln Pro Ser Gly Thr Phe Ala 
        35                  40                  45              


Gly Ser Thr Phe Pro Gly Arg Asp Tyr Ala Trp Val Arg Val Ser Ser 
    50                  55                  60                  


Gly Asn Thr Met Arg Gly Leu Val Asn Arg Tyr Pro Gly Thr Val Pro 
65                  70                  75                  80  


Val Lys Gly Ser Asn Glu Ser Ser Val Gly Ala Ser Val Cys Arg Ser 
                85                  90                  95      


Gly Ser Thr Thr Gly Trp His Cys Gly Thr Ile Gln Gln Lys Asn Thr 
            100                 105                 110         


Ser Val Thr Tyr Pro Glu Gly Thr Ile Ser Gly Val Thr Arg Thr Asn 
        115                 120                 125             


Ala Cys Ala Glu Pro Gly Asp Ser Gly Gly Ser Trp Leu Thr Gly Asp 
    130                 135                 140                 


Gln Ala Gln Gly Val Thr Ser Gly Gly Ser Gly Asn Cys Ser Ser Gly 
145                 150                 155                 160 


Gly Thr Thr Tyr Phe Gln Pro Val Asn Pro Ile Leu Gln Ala Tyr Gly 
                165                 170                 175     


Leu Gln Leu Val Ile Glu Gly Gly Pro Thr 
            180                 185     


<210>  6
<211>  269
<212>  PRT
<213>  Bacillus clausii

<400>  6

Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala 
1               5                   10                  15      


His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 
        115                 120                 125             


Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 
        195                 200                 205             


Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 
                245                 250                 255     


Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  7
<211>  188
<212>  PRT
<213>  Nocardiopsis sp. (protease 10)

<400>  7

Ala Asp Ile Ile Gly Gly Leu Ala Tyr Thr Met Gly Gly Arg Cys Ser 
1               5                   10                  15      


Val Gly Phe Ala Ala Thr Asn Ala Ala Gly Gln Pro Gly Phe Val Thr 
            20                  25                  30          


Ala Gly His Cys Gly Arg Val Gly Thr Gln Val Thr Ile Gly Asn Gly 
        35                  40                  45              


Arg Gly Val Phe Glu Gln Ser Val Phe Pro Gly Asn Asp Ala Ala Phe 
    50                  55                  60                  


Val Arg Gly Thr Ser Asn Phe Thr Leu Thr Asn Leu Val Ser Arg Tyr 
65                  70                  75                  80  


Asn Thr Gly Gly Tyr Ala Thr Val Ala Gly His Asn Gln Ala Pro Ile 
                85                  90                  95      


Gly Ser Ser Val Cys Arg Ser Gly Ser Thr Thr Gly Trp His Cys Gly 
            100                 105                 110         


Thr Ile Gln Ala Arg Gly Gln Ser Val Ser Tyr Pro Glu Gly Thr Val 
        115                 120                 125             


Thr Asn Met Thr Arg Thr Thr Val Cys Ala Glu Pro Gly Asp Ser Gly 
    130                 135                 140                 


Gly Ser Tyr Ile Ser Gly Thr Gln Ala Gln Gly Val Thr Ser Gly Gly 
145                 150                 155                 160 


Ser Gly Asn Cys Arg Thr Gly Gly Thr Thr Phe Tyr Gln Glu Val Thr 
                165                 170                 175     


Pro Met Val Asn Ser Trp Gly Val Arg Leu Arg Thr 
            180                 185             


