               SEQUENCE LISTING

<110> DIREVO Industrisl Biotechnology GmbH

<120> SINGLE-STEP BIOCONVERSION OF LIGNOCELLULOSIC BIOMASS WITH EXTREME THERMOPHILIC BACTERIA

<130> DIR-PA13-PCT

<150> USUS 61/537,892
<151> 2011-09-22
<150> EPEP 11007706
<151> 2011-09-22
<150> USUS 61/669,981
<151> 2012-07-10
<150> EPEP 12175679
<151> 2012-07-10

<160> 9

<170> BiSSAP 1.2

<210> 1
<211> 1396
<212> DNA
<213> Caldicellulosiruptor

<220> 
<221> source
<222> 1..1396
<223> /organism="Caldicellulosiruptor"
      /note="16SrDNA"
      /mol_type="unassigned DNA"

<400> 1
ttacgacttc accccaatca tcagccccac cttcaacaca gcttaacctg tgtcttcagg      60

tgttgctgac tctcatggtg tgacgggcgg tgtgtacaag gcccgggaac gtattcaccg     120

cggcatgctg atccgcgatt actagcgatt ccgacttcat gcaggcgagt tgcagcctgc     180

aatccgaact gggggtgctt ttttgggatt cgctccggct cgcgccttcg cacgccctct     240

gtagcaccca ttgtagcacg tgtgtagccc agggcataag gggcatgatg atttgacgtc     300

atccccacct tcctccgcct catcgacggc agtcccctta gagtgcccac cattacgcgc     360

tggcaactaa gggcaggggt tgcgctcgtt gcgggactta acccaacatc tcacgacacg     420

agctgacgac aaccatgcac cacctgtgtc cgggctcctg ctctcatcga acaggcaccc     480

caccctttcg ggcaggtccc cggcatgtca agccctggta aggttcttcg cgttgcttcg     540

aattaaacca catgctccac cgcttgtgcg ggcccccgtc aattcctttg agtttcaacc     600

ttgcggccgt actccccagg cgggatgctt attgtgttaa ctacggcacg gaggagtcct     660

tctcccccac acctagcatc catcgtttac agcgtggact accagggtat ctaatcctgt     720

tcgctcccca cgctttcgtg cctcagcgtc agttacggtc cagacggccg ccttcgccac     780

tggtgttcct cccgatatct acgcatttca ccgctacacc gggaattccg ccgtcctctc     840

ccgcactcaa gctatgcagt attaagcgca atccttaggt tgagcctaag gctttcacgc     900

ttaactcgca tagccgccta cgcacccttt acgcccagta attccggaca acgctcgcca     960

cctacgtatt accgcggctg ctggcacgta gttagccgtg gctttttaaa cgggtactat    1020

ctcctacttc tccccgtcca aagaggttta caccccgaag ggcttcttcc ctcacgcggc    1080

gtcgctgcgt caggcttccg cccattgcgc aagattcccc gctgctgcct cccgtaggag    1140

tgtgggccgt gtctcagtcc cactgtggcc gtacaccctc tcaggccggc tacccgtcgt    1200

cgccttggta ggccgttacc ccaccaacta gctgatgggc cgcgagccca tccccagcca    1260

gtatagcctc cccggctacc ctttcaccac atcaccatgc gatgacgtgg tcccatcggg    1320

tattagcagc cctttcgagc tgttatcccc gtgctggggg taggttgctc acgtgttact    1380

cacccgtccg ccgcta                                                    1396


<210> 2
<211> 1480
<212> DNA
<213> Caldicellulosiruptor

<220> 
<221> source
<222> 1..1480
<223> /organism="Caldicellulosiruptor"
      /note="16SrDNA"
      /mol_type="unassigned DNA"

<400> 2
ctcaggacga acgctggcgg cgtgcctaac gcatgcaagt cgagcggagg tagccatgaa      60

ggtgaagagc tggagtggct atcttagcgg cggacgggtg agtaacacgt gagcaaccta     120

ccctcagcac ggggataaca gctcgaaagg gctgctaata cccgatggga ccacggcatc     180

gcatgatgtt gtggtgaaag ggtagccgtg gaggctatac cggctgggga tgggctcgcg     240

gcccatcagc tagttggtgg ggtaacggcc taccaaggct acgacgggta gccggcctga     300

gagggtggtc ggccacagtg ggactgagac acggcccaca ctcctacggg aggcagcagc     360

ggggaatctt gcgcaatggg cgaaagcctg acgcagcgac gccgcgtgag ggaggaagcc     420

cttcggggtg taaacctctt tggacgggga gaaggaggag atagtacccg tttaaaaagc     480

cacggctaac tacgtgccag cagccgcggt aatacgtagg tggcgagcgt tgtccggaat     540

tactgggcgt aaagggtgcg taggcggcta tgcaagttaa gcgtgaaatc ttggggctca     600

accccaaggc tgcgcttaat actgcatagc ttgagtgcgg gagaggacgg cggaattccc     660

ggtgtagcgg tgaaatgcgt agatatcggg aggaacacca gtggcgaagg cggccgtctg     720

gaccgtaact gacgctgagg cacgaaagcg tggggagcga acaggattag ataccctggt     780

agtccacgct gtaaacgatg gatgctaggt gtgggggaga aggactcctc cgtgccgtag     840

ttaacacaat aagcatcccg cctggggagt acggccgcaa ggttgaaact caaaggaatt     900

gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct     960

taccagggct tgacatgccg ggaacctgcc cgaaagggtg gggtgcctgc gcgatgagtg    1020

caggagcccg gacacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt    1080

taagtcccgc aacgagcgca acccctgccc ttagttgcca gcacgtaatg gtgggcactc    1140

taaggggact gccgccgatg aggcggagga aggtggggat gacgtcaaat catcatgccc    1200

cttatgccct gggctacaca cgtgctacaa tgggtgctac agagggttgc gaaggcgcga    1260

gccggagcta atcccaaaaa agcaccccca gttcggattg caggctgcaa ctcgcctgca    1320

tgaagtcgga atcgctagta atcgcggatc agcatgccgc ggtgaatacg ttcccgggcc    1380

ttgtacacac cgcccgtcac accatgagag tcagcaacac ctgaagacac agggcagctg    1440

tgttgaaggt ggggctgatg attggggtga agtcgtaaca                          1480


<210> 3
<211> 1481
<212> DNA
<213> Caldicellulosiruptor

<220> 
<221> source
<222> 1..1481
<223> /organism="Caldicellulosiruptor"
      /note="16SrDNA"
      /mol_type="unassigned DNA"

<400> 3
tcaggacgaa cgctggcggc gtgcctaacg catgcaagtc gagcggagat ggtggttgaa      60

ggtgatgagc tggaggctgc catcttagcg gcggacgggt gagtaacacg tgagcaacct     120

acccccagca cggggataac agctcgaaag ggctgctaat acccgatggg accacgtcat     180

cgcatggtga tgtggtgaaa gggtagccgg ggaggctata ctggctgggg atgggctcgc     240

ggcccatcag ctagttggtg gggtaacggc tcaccaaggc gacgacgggt agccggcctg     300

agagggtgta cggccacagt gggactgaga cacggcccac actcctacgg gaggcagcag     360

cggggaatct tgcgcaatgg gcggaagcct gacgcagcga cgccgcgtga gggaagaagc     420

ccttcggggt gtaaacctct ttggacgggg agaagtagga gatagtaccc gtttaaaaag     480

ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcgagcg ttgtccggaa     540

ttactgggcg taaagggtgc gtaggcggct atgcgagtta agcgtgaaag ccttaggctc     600

aacctaagga ttgcgcttaa tactgcatag cttgagtgcg ggagaggacg gcggaattcc     660

cggtgtagcg gtgaaatgcg tagatatcgg gaggaacacc agtggcgaag gcggccgtct     720

ggaccgtaac tgacgctgag gcacgaaagc gtggggagcg aacaggatta gataccctgg     780

tagtccacgc tgtaaacgat ggatgctagg tgtgggggag aaggactctt ccgtgccgta     840

gttaacacaa taagcatccc gcctggggag tacggccgca aggttgaaac tcaaaggaat     900

tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc     960

ttaccagggc ttgacatgcc ggggacctgc ccgaaagggt ggggtgcctg ttcgatgaga    1020

gcaggaaccc ggacacaggt ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg    1080

ttaagtcccg caacgagcgc aacccctgcc cttagttgcc agcgggtaat ggtgggcact    1140

ctaaggggac tgccgtcgat gaggcggagg aaggtgggga tgacgtcaaa tcatcatgcc    1200

ccttatgccc tgggctacac acgtgctaca atgggtgcta cagagggcgt gcgaaggcgc    1260

gagccggagc gaatcccaaa aaagcacccc cagttcggat tgcaggctgc aactcgcctg    1320

catgaagtcg gaatcgctag taatcgcgga tcagcatgcc gcggtgaata cgttcccggg    1380

ccttgtacac accgcccgtc acaccatgag agtcagcaac acctgaagac acaggttaag    1440

ctgtgttgaa ggtggggctg atgattgggg tgaagtcgta a                        1481


<210> 4
<211> 1212
<212> DNA
<213> Caldicellulosiruptor

<220> 
<221> source
<222> 1..1212
<223> /organism="Caldicellulosiruptor"
      /note="16SrDNA"
      /mol_type="unassigned DNA"

<400> 4
cctgtgtctt caggtgttgc tgactctcat ggtgtgacgg gcggtgtgta caaggcccgg      60

gaacgtattc accgcggcat gctgatccgc gattactagc gattccgact tcatgcaggc     120

gagttgcagc ctgcaatccg aactgggggt gcttttttgg gattcgctcc ggctcgcgcc     180

ttcgcacgcc ctctgtagca cccattgtag cacgtgtgta gcccagggca taaggggcat     240

gatgatttga cgtcatcccc accttcctcc gcctcatcga cggcagtccc cttagagtgc     300

ccaccattac gcgctggcaa ctaagggcag gggttgcgct cgttgcggga cttaacccaa     360

catctcacga cacgagctga cgacaaccat gcaccacctg tgtccgggct cctgctctca     420

tcgaacaggc accccaccct ttcgggcagg tccccggcat gtcaagccct ggtaaggttc     480

ttcgcgttgc ttcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattcc     540

tttgagtttc aaccttgcgg ccgtactccc caggcgggat gcttattgtg ttaactacgg     600

cacggaggag tccttctccc ccacacctag catccatcgt ttacagcgtg gactaccagg     660

gtatctaatc ctgttcgctc cccacgcttt cgtgcctcag cgtcagttac ggtccagacg     720

gccgccttcg ccactggtgt tcctcccgat atctacgcat ttcaccgcta caccgggaat     780

tccgccgtcc tctcccgcac tcaagctatg cagtattaag cgcaatcctt aggttgagcc     840

taaggctttc acgcttaact cgcatagccg cctacgcacc ctttacgccc agtaattccg     900

gacaacgctc gccacctacg tattaccgcg gctgctggca cgtagttagc cgtggctttt     960

taaacgggta ctatctccta cttctccccg tccaaagagg tttacacccc gaagggcttc    1020

ttccctcacg cggcgtcgct gcgtcaggct tccgcccatt gcgcaagatt ccccgctgct    1080

gcctcccgta ggagtgtggg ccgtgtctca gtcccactgt ggccgtacac cctctcaggc    1140

cggctacccg tcgtcgcctt ggtaggccgt taccccacca actagctgat gggccgcgag    1200

cccatcccca gc                                                        1212


<210> 5
<211> 1253
<212> DNA
<213> Caldicellulosiruptor

<220> 
<221> source
<222> 1..1253
<223> /organism="Caldicellulosiruptor"
      /note="16SrDNA"
      /mol_type="unassigned DNA"

<400> 5
cgacttcacc ccaatcatca gccccacctt caacacagct taacctgtgt cttcaggtgt      60

tgctgactct catggtgtga cgggcggtgt gtacaaggcc cgggaacgta ttcaccgcgg     120

catgctgatc cgcgattact agcgattccg acttcatgca ggcgagttgc agcctgcaat     180

ccgaactggg ggtgcttttt tgggattcgc tccggctcgc gccttcgcac gccctctgta     240

gcacccattg tagcacgtgt gtagcccagg gcataagggg catgatgatt tgacgtcatc     300

cccaccttcc tccgcctcat cgacggcagt ccccttagag tgcccaccat tacgcgctgg     360

caactaaggg caggggttgc gctcgttgcg ggacttaacc caacatctca cgacacgagc     420

tgacgacaac catgcaccac ctgtgtccgg gctcctgctc tcatcgaaca ggcaccccac     480

cctttcgggc aggtccccgg catgtcaagc cctggtaagg ttcttcgcgt tgcttcgaat     540

taaaccacat gctccaccgc ttgtgcgggc ccccgtcaat tcctttgagt ttcaaccttg     600

cggccgtact ccccaggcgg gatgcttatt gtgttaacta cggcacggag gagtccttct     660

cccccacacc tagcatccat cgtttacagc gtggactacc agggtatcta atcctgttcg     720

ctccccacgc tttcgtgcct cagcgtcagt tacggtccag acggccgcct tcgccactgg     780

tgttcctccc gatatctacg catttcaccg ctacaccggg aattccgccg tcctctcccg     840

cactcaagct atgcagtatt aagcgcaatc cttaggttga gcctaaggct ttcacgctta     900

actcgcatag ccgcctacgc accctttacg cccagtaatt ccggacaacg ctcgccacct     960

acgtattacc gcggctgctg gcacgtagtt agccgtggct ttttaaacgg gtactatctc    1020

ctacttctcc ccgtccaaag aggtttacac cccgaagggc ttcttccctc acgcggcgtc    1080

gctgcgtcag gcttccgccc attgcgcaag attccccgct gctgcctccc gtaggagtgt    1140

gggccgtgtc tcagtcccac tgtggccgta caccctctca ggccggctac ccgtcgtcgc    1200

cttggtaagc cgttacccca ccaactagct gatgggccgc gagcccatcc cca           1253


<210> 6
<211> 1255
<212> DNA
<213> Caldicellulosiruptor

<220> 
<221> source
<222> 1..1255
<223> /organism="Caldicellulosiruptor"
      /note="16SrDNA"
      /mol_type="unassigned DNA"

<400> 6
gacttcaccc caatcatcag ccccaccttc aacacagctt aacctgtgtc ttcaggtgtt      60

gctgactctc atggtgtgac gggcggtgtg tacaaggccc gggaacgtat tcaccgcggc     120

atgctgatcc gcgattacta gcgattccga cttcatgcag gcgagttgca gcctgcaatc     180

cgaactgggg gtgctttttt gggattcgct ccggctcgcg ccttcgcacg ccctctgtag     240

cacccattgt agcacgtgtg tagcccaggg cataaggggc atgatgattt gacgtcatcc     300

ccaccttcct ccgcctcatc gacggcagtc cccttagagt gcccaccatt acgcgctggc     360

aactaagggc aggggttgcg ctcgttgcgg gacttaaccc aacatctcac gacacgagct     420

gacgacaacc atgcaccacc tgtgtccggg ctcctgctct catcgaacag gcaccccacc     480

ctttcgggca ggtccccggc atgtcaagcc ctggtaaggt tcttcgcgtt gcttcgaatt     540

aaaccacatg ctccaccgct tgtgcgggcc cccgtcaatt cctttgagtt tcaaccttgc     600

ggccgtactc cccaggcggg atgcttattg tgttaactac ggcacggaag agtccttctc     660

ccccacacct agcatccatc gtttacagcg tggactacca gggtatctaa tcctgttcgc     720

tccccacgct ttcgtgcctc agcgtcagtt acggtccaga cggccgcctt cgccactggt     780

gttcctcccg atatctacgc atttcaccgc tacaccggga attccgccgt cctctcccgc     840

actcaagcta tgcagtatta agcgcaatcc ttaggttgag cctaaggctt tcacgcttaa     900

ctcgcatagc cgcctacgca ccctttacgc ccagtaattc cggacaacgc tcgccaccta     960

cgtattaccg cggctgctgg cacgtagtta gccgtggctt tttaaacggg tactatctcc    1020

tacttctccc cgtccaaaga ggtttacacc ccgaagggct tcttccctca cgcggcgtcg    1080

ctgcgtcagg cttccgccca ttgcgcaaga ttccccgctg ctgcctcccg taggagtgtg    1140

ggccgtgtct cagtcccact gtggccgtac accctctcag gccggctacc cgtcgtcgcc    1200

ttggtgagcc gttaccccac caactagctg atgggccgcg agcccatccc cagcc         1255


<210> 7
<211> 1466
<212> DNA
<213> Caldicellulosiruptor

<220> 
<221> source
<222> 1..1466
<223> /organism="Caldicellulosiruptor"
      /note="16SrDNA"
      /mol_type="unassigned DNA"

<400> 7
gacttcaccc ccaatcatca gccccacctt caacacagct taacctgtgt cttcaggtgt      60

tgctgactct catggtgtga cgggcggtgt gtacaaggcc cgggaacgta ttcaccgcgg     120

catgctgatc cgcgattact agcgattccg acttcatgca ggcgagttgc agcctgcaat     180

ccgaactggg ggtgcttttt tgggattcgc tccggctcgc gccttcgcac gccctctgta     240

gcacccattg tagcacgtgt gtagcccagg gcataagggg catgatgatt tgacgtcatc     300

cccaccttcc tccgcctcat cgacggcagt ccccttagag tgcccaccat tacgcgctgg     360

caactaaggg caggggttgc gctcgttgcg ggacttaacc caacatctca cgacacgagc     420

tgacgacaac catgcaccac ctgtgtccgg gctcctgctc tcatcgaaca ggcaccccac     480

cctttcgggc aggtccccgg catgtcaagc cctggtaagg ttcttcgcgt tgcttcgaat     540

taaaccacat gctccaccgc ttgtgcgggc ccccgtcaat tcctttgagt ttcaaccttg     600

cggccgtact ccccaggcgg gatgcttatt gtgttaacta cggcacggag gagtccttct     660

cccccacacc tagcatccat cgtttacagc gtggactacc agggtatcta atcctgttcg     720

ctccccacgc tttcgtgcct cagcgtcagt tacggtccag acggccgcct tcgccactgg     780

tgttcctccc gatatctacg catttcaccg ctacaccggg aattccgccg tcctctcccg     840

cactcaagct atgcagtatt aagcgcaatc cttaggttga gcctaaggct ttcacgctta     900

actcgcatag ccgcctacgc accctttacg cccagtaatt ccggacaacg ctcgccacct     960

acgtattacc gcggctgctg gcacgtagtt agccgtggct ttttaaacgg gtactatctc    1020

ctacttctcc ccgtccaaag aggtttacac cccgaagggc ttcttccctc acgcggcgtc    1080

gctgcgtcag gcttccgccc attgcgcaag attccccgct gctgcctccc gtaggagtgt    1140

gggccgtgtc tcagtcccac tgtggccgta caccctctca ggccggctac ccgtcgtcgc    1200

cttggtgagc cgttacctca ccaactagct gatgggccgc gagcccatcc ccagccggat    1260

tactcctttc accacatcac catgcgatga cgtggtccca tcgggtatta gcagcccttt    1320

cgagctgtta tccccgtgct gggggtaggt tgctcacgtg ttactcaccc gtccgccgct    1380

aagatggcag cctccagctc atcaccttca accaccatct ccgctcgact tgcatgcgtt    1440

aggcacgccg ccagcgttcg tcctga                                         1466


<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Primer for 16SrDNA amplification"
      /mol_type="unassigned DNA"

<400> 8
agagtttgat cmtggctcag                                                20


<210> 9
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..19
<223> /organism="Artificial Sequence"
      /note="16SrDNA reverse Primer"
      /mol_type="unassigned DNA"

<400> 9
ggttaccttg ttacgactt                                                 19


