                         SEQUENCE LISTING

<110>  Regents of the University of Minnesota
       SHEN, Wei
 
<120>  DEVICES AND METHODS RELATING TO IMMOBILIZED LIGANDS

<130>  110.03550201

<150>  61/533,441
<151>  2011-09-12

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  98
<212>  PRT
<213>  artificial

<220>
<223>  immobilized immobilized polypeptide containing A domain and 
       bioactive ligand

<400>  1

Met Arg Gly Ser His His His His His His Gly Ser Asp Asp Asp Asp 
1               5                   10                  15      


Lys Ala Ser Ser Gly Ser Gly Cys Ser Gly Ser Gly Thr Ser Gly Asp 
            20                  25                  30          


Leu Glu Asn Glu Val Ala Gln Leu Glu Arg Glu Val Arg Ser Leu Glu 
        35                  40                  45              


Asp Glu Ala Ala Glu Leu Glu Gln Lys Val Ser Arg Leu Lys Asn Glu 
    50                  55                  60                  


Ile Glu Asp Leu Lys Ala Glu Ile Gly Asp His Val Ala Pro Arg Asp 
65                  70                  75                  80  


Thr Ser Tyr Ala Val Thr Gly Arg Gly Asp Ser Pro Ala Ser Ser Thr 
                85                  90                  95      


Ser Trp 
        


<210>  2
<211>  79
<212>  PRT
<213>  artificial

<220>
<223>  polypeptide containing domain B conjugated to PEG (B-PEG)

<400>  2

Met Arg Gly Ser His His His His His His Gly Ser Asp Asp Asp Asp 
1               5                   10                  15      


Lys Trp Ala Ser Gly Thr Ser Gly Asp Leu Lys Asn Lys Val Ala Gln 
            20                  25                  30          


Leu Lys Arg Lys Val Arg Ser Leu Lys Asp Lys Ala Ala Glu Leu Lys 
        35                  40                  45              


Gln Glu Val Ser Arg Leu Glu Asn Glu Ile Glu Asp Leu Lys Ala Lys 
    50                  55                  60                  


Ile Gly Asp His Val Ala Pro Arg Asp Thr Ser Met Gly Gly Cys 
65                  70                  75                  


<210>  3
<211>  72
<212>  PRT
<213>  artificial

<220>
<223>  non-immobilized polypeptide containing domain A

<400>  3

Met Arg Gly Ser His His His His His His Gly Ser Asp Asp Asp Asp 
1               5                   10                  15      


Lys Ala Ser Gly Asp Leu Glu Asn Glu Val Ala Gln Leu Glu Arg Glu 
            20                  25                  30          


Val Arg Ser Leu Glu Asp Glu Ala Ala Glu Leu Glu Gln Lys Val Ser 
        35                  40                  45              


Arg Leu Lys Asn Glu Ile Glu Asp Leu Lys Ala Glu Ile Gly Asp His 
    50                  55                  60                  


Val Ala Pro Arg Asp Thr Ser Trp 
65                  70          


<210>  4
<211>  83
<212>  PRT
<213>  artificial

<220>
<223>  immobilized polypeptide containing domain A

<400>  4

Met Arg Gly Ser His His His His His His Gly Ser Asp Asp Asp Asp 
1               5                   10                  15      


Lys Ala Ser Ser Gly Ser Gly Cys Ser Gly Ser Gly Thr Ser Gly Asp 
            20                  25                  30          


Leu Glu Asn Glu Val Ala Gln Leu Glu Arg Glu Val Arg Ser Leu Glu 
        35                  40                  45              


Asp Glu Ala Ala Glu Leu Glu Gln Lys Val Ser Arg Leu Lys Asn Glu 
    50                  55                  60                  


Ile Glu Asp Leu Lys Ala Glu Ile Gly Asp His Val Ala Pro Arg Asp 
65                  70                  75                  80  


Thr Ser Trp 
            


<210>  5
<211>  148
<212>  PRT
<213>  artificial

<220>
<223>  immobilized polypeptide containing C(10) domain and bioactive 
       ligand

<400>  5

Met Arg Gly Ser His His His His His His Gly Ser Asp Asp Asp Asp 
1               5                   10                  15      


Lys Ala Ser Ser Gly Ser Gly Cys Ser Gly Ser Gly Thr Ser Tyr Arg 
            20                  25                  30          


Asp Pro Met Gly Ala Gly Ala Gly Ala Gly Pro Glu Gly Ala Gly Ala 
        35                  40                  45              


Gly Ala Gly Pro Glu Gly Ala Gly Ala Gly Ala Gly Pro Glu Gly Ala 
    50                  55                  60                  


Gly Ala Gly Ala Gly Pro Glu Gly Ala Gly Ala Gly Ala Gly Pro Glu 
65                  70                  75                  80  


Gly Ala Gly Ala Gly Ala Gly Pro Glu Gly Ala Gly Ala Gly Ala Gly 
                85                  90                  95      


Pro Glu Gly Ala Gly Ala Gly Ala Gly Pro Glu Gly Ala Gly Ala Gly 
            100                 105                 110         


Ala Gly Pro Glu Gly Ala Gly Ala Gly Ala Gly Pro Glu Gly Ala Arg 
        115                 120                 125             


Met Pro Thr Ser Tyr Ala Val Thr Gly Arg Gly Asp Ser Pro Ala Ser 
    130                 135                 140                 


Ser Thr Ser Trp 
145             


<210>  6
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  histidine peptide domain

<400>  6

His His His His His His 
1               5       


<210>  7
<211>  42
<212>  PRT
<213>  artificial

<220>
<223>  polypeptide A - hydrophilic random coil domain

<400>  7

Ser Gly Asp Leu Glu Asn Glu Val Ala Gln Leu Glu Arg Glu Val Arg 
1               5                   10                  15      


Ser Leu Glu Asp Glu Ala Ala Glu Leu Glu Gln Lys Val Ser Arg Leu 
            20                  25                  30          


Lys Asn Glu Ile Glu Asp Leu Lys Ala Glu 
        35                  40          


<210>  8
<211>  42
<212>  PRT
<213>  artificial

<220>
<223>  polypeptide B

<400>  8

Ser Gly Asp Leu Lys Asn Lys Val Ala Gln Leu Lys Arg Lys Val Arg 
1               5                   10                  15      


Ser Leu Lys Asp Lys Ala Ala Glu Leu Lys Gln Glu Val Ser Arg Leu 
            20                  25                  30          


Glu Asn Glu Ile Glu Asp Leu Lys Ala Lys 
        35                  40          


<210>  9
<211>  90
<212>  PRT
<213>  artificial

<220>
<223>  polypeptide C(10)

<400>  9

Ala Gly Ala Gly Ala Gly Pro Glu Gly Ala Gly Ala Gly Ala Gly Pro 
1               5                   10                  15      


Glu Gly Ala Gly Ala Gly Ala Gly Pro Glu Gly Ala Gly Ala Gly Ala 
            20                  25                  30          


Gly Pro Glu Gly Ala Gly Ala Gly Ala Gly Pro Glu Gly Ala Gly Ala 
        35                  40                  45              


Gly Ala Gly Pro Glu Gly Ala Gly Ala Gly Ala Gly Pro Glu Gly Ala 
    50                  55                  60                  


Gly Ala Gly Ala Gly Pro Glu Gly Ala Gly Ala Gly Ala Gly Pro Glu 
65                  70                  75                  80  


Gly Ala Gly Ala Gly Ala Gly Pro Glu Gly 
                85                  90  


<210>  10
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  immobilized nucleotide

<400>  10
tgagtgtacc accattggaa g                                                 21


<210>  11
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  non-immobilized nucleotide

<400>  11
cttccaatgg tggtacactc a                                                 21


<210>  12
<211>  4
<212>  PRT
<213>  artificial

<220>
<223>  peptide ligand

<400>  12

Arg Glu Asp Val 
1               


<210>  13
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  peptide ligand

<400>  13

Ser Gly Ser Gly Ser Gly Arg Glu Asp Val 
1               5                   10  


