                         SEQUENCE LISTING

<110>  WONG, SUI-LAM
       WU, SAU-CHING
       BARRETTE-NG, ISABELLE
       NG, KENNETH K-S
 
<120>  STREPTAVIDIN MUTEIN EXHIBITING REVERSIBLE BINDING FOR BIOTIN AND 
       STREPTAVIDIN BINDING PEPTIDE TAGGED PROTEINS

<130>  UNTI.P0124WO

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  159
<212>  PRT
<213>  streptavidin mutein

<400>  1

Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly 
1               5                   10                  15      


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
            20                  25                  30          


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Gly 
        35                  40                  45              


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
            100                 105                 110         


Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys 
    130                 135                 140                 


Lys Ala Gly Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
145                 150                 155                 


<210>  2
<211>  480
<212>  DNA
<213>  streptavidin mutein

<400>  2
gacccgagca aagattctaa agcacaagta tctgctgcag aagcgggcat tacgggcacg       60

tggtataatc agctgggcgc tacgtttatt gttacggccg gcgcagatgg agctctgacg      120

ggcacgtatg aaagcgcggt tacaaatgca gaatctagat acgttcttac aggaagatat      180

gattctgcac ctgcaacaga tggatccggc acggcactgg gctggacagt tgcatggaaa      240

aacaattatc gcaacgcaca tagcgccacg acgtggtctg gccaatatgt tggcggtgca      300

gaagcacgca ttaacacaca gtggcttctg acgtccggaa caacagaagc aaatgcatgg      360

aaaagtactc ttgttggaca tgatacattt acaaaagtta aacctagcgc agcatctatc      420

gatgcagcga aaaaagcagg agttaacaat ggcaatcctt tagatgcagt tcaacaataa      480


<210>  3
<211>  159
<212>  PRT
<213>  streptavidin mutein

<400>  3

Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly 
1               5                   10                  15      


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ala Thr Phe Ile Val Thr 
            20                  25                  30          


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Thr 
        35                  40                  45              


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
            100                 105                 110         


Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys 
    130                 135                 140                 


Lys Ala Gly Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
145                 150                 155                 


<210>  4
<211>  567
<212>  DNA
<213>  streptavidin mutein

<400>  4
atgaatatca aaaaattcgc taagcaagct acagtcctta catttacaac agcgctgctt       60

gccgggggcg ccacccaggc ctttgctgac ccgagcaaag attctaaagc acaagtatct      120

gctgcagaag cgggcattac gggcacgtgg tataatcagc tgggcgctac gtttattgtt      180

acggccggcg cagatggagc tctgacgggc acgtatgaaa gcgcggttac aaatgcagaa      240

tctagatacg ttcttacagg aagatatgat tctgcacctg caacagatgg atccggcacg      300

gcactgggct ggacagttgc atggaaaaac aattatcgca acgcacatag cgccacgacg      360

tggtctggcc aatatgttgg cggtgcagaa gcacgcatta acacacagtg gcttctgacg      420

tccggaacaa cagaagcaaa tgcatggaaa agtactcttg ttggacatga tacatttaca      480

aaagttaaac ctagcgcagc atctatcgat gcagcgaaaa aagcaggagt taacaatggc      540

aatcctttag atgcagttca acaataa                                          567


<210>  5
<211>  188
<212>  PRT
<213>  streptavidin mutein

<400>  5

Met Asn Ile Lys Lys Phe Ala Lys Gln Ala Thr Val Leu Thr Phe Thr 
1               5                   10                  15      


Thr Ala Leu Leu Ala Gly Gly Ala Thr Gln Ala Phe Ala Asp Pro Ser 
            20                  25                  30          


Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly Ile Thr Gly 
        35                  40                  45              


Thr Trp Tyr Asn Gln Leu Gly Ala Thr Phe Ile Val Thr Ala Gly Ala 
    50                  55                  60                  


Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Thr Asn Ala Glu 
65                  70                  75                  80  


Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro Ala Thr Asp 
                85                  90                  95      


Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys Asn Asn Tyr 
            100                 105                 110         


Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr Val Gly Gly 
        115                 120                 125             


Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser Gly Thr Thr 
    130                 135                 140                 


Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp Thr Phe Thr 
145                 150                 155                 160 


Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys Lys Ala Gly 
                165                 170                 175     


Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
            180                 185             


<210>  6
<211>  130
<212>  PRT
<213>  streptavidin mutein

<400>  6

Ala Ala Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ala 
1               5                   10                  15      


Thr Phe Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr 
            20                  25                  30          


Glu Ser Ala Val Thr Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg 
        35                  40                  45              


Tyr Asp Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp 
    50                  55                  60                  


Thr Val Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr 
65                  70                  75                  80  


Trp Ser Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln 
                85                  90                  95      


Trp Leu Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr 
            100                 105                 110         


Leu Val Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125             


Ile Asp 
    130 


<210>  7
<211>  390
<212>  DNA
<213>  streptavidin mutein

<400>  7
gctgcagaag cgggcattac gggcacgtgg tataatcagc tgggcgctac gtttattgtt       60

acggccggcg cagatggagc tctgacgggc acgtatgaaa gcgcggttac aaatgcagaa      120

tctagatacg ttcttacagg aagatatgat tctgcacctg caacagatgg atccggcacg      180

gcactgggct ggacagttgc atggaaaaac aattatcgca acgcacatag cgccacgacg      240

tggtctggcc aatatgttgg cggtgcagaa gcacgcatta acacacagtg gcttctgacg      300

tccggaacaa cagaagcaaa tgcatggaaa agtactcttg ttggacatga tacatttaca      360

aaagttaaac ctagcgcagc atctatcgat                                       390


<210>  8
<211>  126
<212>  PRT
<213>  streptavidin mutein

<400>  8

Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ala Thr Phe 
1               5                   10                  15      


Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser 
            20                  25                  30          


Ala Val Thr Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp 
        35                  40                  45              


Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val 
    50                  55                  60                  


Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser 
65                  70                  75                  80  


Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu 
                85                  90                  95      


Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val 
            100                 105                 110         


Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125     


<210>  9
<211>  378
<212>  DNA
<213>  streptavidin mutein

<400>  9
gaagcgggca ttacgggcac gtggtataat cagctgggcg ctacgtttat tgttacggcc       60

ggcgcagatg gagctctgac gggcacgtat gaaagcgcgg ttacaaatgc agaatctaga      120

tacgttctta caggaagata tgattctgca cctgcaacag atggatccgg cacggcactg      180

ggctggacag ttgcatggaa aaacaattat cgcaacgcac atagcgccac gacgtggtct      240

ggccaatatg ttggcggtgc agaagcacgc attaacacac agtggcttct gacgtccgga      300

acaacagaag caaatgcatg gaaaagtact cttgttggac atgatacatt tacaaaagtt      360

aaacctagcg cagcatct                                                    378


<210>  10
<211>  59
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  10

Cys Gly Gly Gly Gly Ser Thr Ser Gly Gly Ser Thr Ser Gly Gly Ser 
1               5                   10                  15      


Thr Ser Gly Gly Gly Met Asp Glu Lys Thr Thr Gly Trp Arg Gly Gly 
            20                  25                  30          


His Val Val Glu Gly Leu Ala Gly Glu Leu Glu Gln Leu Arg Ala Arg 
        35                  40                  45              


Leu Glu His His Pro Gln Gly Gln Arg Glu Pro 
    50                  55                  


<210>  11
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  11

Gly His Val Val Glu Gly 
1               5       


<210>  12
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  12

Leu Glu His His Pro Gln Gly Gln 
1               5               


<210>  13
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  13

Leu Ala Gly Glu Leu Glu Gln Leu Arg Ala Arg 
1               5                   10      


