                         SEQUENCE LISTING

<110>  The Trustess of Dartmouth College
 
<120>  Production of Butanols in Thermophilic Organisms

<130>  525929

<150>  61/529,701 61/558,099
<151>  2011-08-31 2012-01-10

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  1179
<212>  DNA
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  1
atgaaagaag tagttattgc aagtggtgta aggactgctg tcgggaaatt tggtggaacg       60

cttctaaatg tacctgcagt agatttaggt gctgtagtaa taaaagaagc attaaaaaga      120

gccaatgtga aacctgaaga tgttagtgaa gtgataatgg gaaatgtatt gcaggcaggt      180

cttgggcaga accccgcaag acaagctgaa ataaaagcgg gcataccagt agaagttccg      240

gctatgactg taaacatggt atgtggatca ggtcttagag ctgtgacact tgctgctcag      300

gcagttatgc ttggtgatgc tgacattgtt gtagccggtg gaatggaaaa tatgtcaaga      360

gcaccatata tattaaatga tgctcgcttt gggtacagga tgaacaatgg ccagcttgta      420

gatgaaatgg tatatgatgg tttaacagat gtttttaacc aatatcacat gggaatcact      480

gccgaaaatc ttgctgaaaa atacggcata tcaagagaag agcaggatga atttgcatat      540

agaagccaaa aattagcgtc agaagcgata tcatcaggaa gatttgagga tgagatagtt      600

cctgtgattg tgccgcagaa aaaaggtgaa ccgatagaat ttaaagttga tgaacatgtg      660

agacctaata cgacaattga agcacttgca aaattaaaac cagcattcaa aaaagatgga      720

actgtaactg ctggaaatgc atcaggaatt aacgatgcag ctgcagcagt agttgtgatg      780

tcaaaagaaa aggctgatga acttggaata aagccattag caacgattaa atcatttggt      840

tatgcaggtg ttgaccccag catcatggga attggtccag tatatgctac gagaaaggca      900

ttagaaaaag ctaatctaac tgtagatgat ttagatttaa ttgaagcaaa tgaagcattt      960

gcagcacaat cactggctgt tgcaaaagaa ttaaaattta atatggacag agtgaatgta     1020

aatggtggcg caattgcgat aggtcatcca atcggcgcca gcggatgtag aattctagtg     1080

acgcttttat atgagatgca gaagaggaat tcgcatactg gacttgcaac attgtgcatc     1140

ggcggaggaa tgggaatagc aatggttgtc gaaagatag                            1179


<210>  2
<211>  392
<212>  PRT
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  2

Met Lys Glu Val Val Ile Ala Ser Gly Val Arg Thr Ala Val Gly Lys 
1               5                   10                  15      


Phe Gly Gly Thr Leu Leu Asn Val Pro Ala Val Asp Leu Gly Ala Val 
            20                  25                  30          


Val Ile Lys Glu Ala Leu Lys Arg Ala Asn Val Lys Pro Glu Asp Val 
        35                  40                  45              


Ser Glu Val Ile Met Gly Asn Val Leu Gln Ala Gly Leu Gly Gln Asn 
    50                  55                  60                  


Pro Ala Arg Gln Ala Glu Ile Lys Ala Gly Ile Pro Val Glu Val Pro 
65                  70                  75                  80  


Ala Met Thr Val Asn Met Val Cys Gly Ser Gly Leu Arg Ala Val Thr 
                85                  90                  95      


Leu Ala Ala Gln Ala Val Met Leu Gly Asp Ala Asp Ile Val Val Ala 
            100                 105                 110         


Gly Gly Met Glu Asn Met Ser Arg Ala Pro Tyr Ile Leu Asn Asp Ala 
        115                 120                 125             


Arg Phe Gly Tyr Arg Met Asn Asn Gly Gln Leu Val Asp Glu Met Val 
    130                 135                 140                 


Tyr Asp Gly Leu Thr Asp Val Phe Asn Gln Tyr His Met Gly Ile Thr 
145                 150                 155                 160 


Ala Glu Asn Leu Ala Glu Lys Tyr Gly Ile Ser Arg Glu Glu Gln Asp 
                165                 170                 175     


Glu Phe Ala Tyr Arg Ser Gln Lys Leu Ala Ser Glu Ala Ile Ser Ser 
            180                 185                 190         


Gly Arg Phe Glu Asp Glu Ile Val Pro Val Ile Val Pro Gln Lys Lys 
        195                 200                 205             


Gly Glu Pro Ile Glu Phe Lys Val Asp Glu His Val Arg Pro Asn Thr 
    210                 215                 220                 


Thr Ile Glu Ala Leu Ala Lys Leu Lys Pro Ala Phe Lys Lys Asp Gly 
225                 230                 235                 240 


Thr Val Thr Ala Gly Asn Ala Ser Gly Ile Asn Asp Ala Ala Ala Ala 
                245                 250                 255     


Val Val Val Met Ser Lys Glu Lys Ala Asp Glu Leu Gly Ile Lys Pro 
            260                 265                 270         


Leu Ala Thr Ile Lys Ser Phe Gly Tyr Ala Gly Val Asp Pro Ser Ile 
        275                 280                 285             


Met Gly Ile Gly Pro Val Tyr Ala Thr Arg Lys Ala Leu Glu Lys Ala 
    290                 295                 300                 


Asn Leu Thr Val Asp Asp Leu Asp Leu Ile Glu Ala Asn Glu Ala Phe 
305                 310                 315                 320 


Ala Ala Gln Ser Leu Ala Val Ala Lys Glu Leu Lys Phe Asn Met Asp 
                325                 330                 335     


Arg Val Asn Val Asn Gly Gly Ala Ile Ala Ile Gly His Pro Ile Gly 
            340                 345                 350         


Ala Ser Gly Cys Arg Ile Leu Val Thr Leu Leu Tyr Glu Met Gln Lys 
        355                 360                 365             


Arg Asn Ser His Thr Gly Leu Ala Thr Leu Cys Ile Gly Gly Gly Met 
    370                 375                 380                 


Gly Ile Ala Met Val Val Glu Arg 
385                 390         


<210>  3
<211>  871
<212>  DNA
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  3
atgcaaaaga tttgtgtaat aggtgctgga acaatgggct caggcatcgc tcaagtattt       60

gcacaaaatg gctttgaagt aattttacgc gatattgata tgaagttcgt agaaaaagga      120

tttggcacaa ttgaaaaaaa tttacaaaga aatgttgaca aagggaaaat tacagcagat      180

gagaaaaacg aaattttaag cagaatcaga ggtacaacaa atttggaaga cgcaaaagaa      240

gcagattttg tagttgaagc ggctatagaa aatatggatc tcaagaaaca aatattcaaa      300

gagctagatg aaatatgcaa aatggaaaca atccttgcgt caaatacatc atcactatcc      360

ataacagaaa tagcaagtgc gacaaaaaga cctgagaaag tcataggaat gcatttcttc      420

aacccagttc cagtaatgaa acttgttgaa gtcataaaag gattaaagac atcagagcaa      480

acatttaatg tcgtcagaga attggcttta aaagtagaca aaacacctat agaggtcaaa      540

gaagcacctg gatttgttgt aaataggatt ttaatcccaa tgattaatga agcaattgga      600

atacttgccg atggtgttgc aactgcagaa gacatagatg aagctatgaa acttggtgca      660

aatcatccaa taggaccttt ggcattgtct gatttgatag gcaatgacgt cgttcttgct      720

ataatgaatg tgctttatga agagtacggc gattcgaaat acagaccaca tccacttcta      780

aaaaagatgg taagaggcgg attgctgggt agaaaaactg gcaaaggttt ctttgaatac      840

aaataatctt ttaaggagga gaatatcatg a                                     871


<210>  4
<211>  281
<212>  PRT
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  4

Met Gln Lys Ile Cys Val Ile Gly Ala Gly Thr Met Gly Ser Gly Ile 
1               5                   10                  15      


Ala Gln Val Phe Ala Gln Asn Gly Phe Glu Val Ile Leu Arg Asp Ile 
            20                  25                  30          


Asp Met Lys Phe Val Glu Lys Gly Phe Gly Thr Ile Glu Lys Asn Leu 
        35                  40                  45              


Gln Arg Asn Val Asp Lys Gly Lys Ile Thr Ala Asp Glu Lys Asn Glu 
    50                  55                  60                  


Ile Leu Ser Arg Ile Arg Gly Thr Thr Asn Leu Glu Asp Ala Lys Glu 
65                  70                  75                  80  


Ala Asp Phe Val Val Glu Ala Ala Ile Glu Asn Met Asp Leu Lys Lys 
                85                  90                  95      


Gln Ile Phe Lys Glu Leu Asp Glu Ile Cys Lys Met Glu Thr Ile Leu 
            100                 105                 110         


Ala Ser Asn Thr Ser Ser Leu Ser Ile Thr Glu Ile Ala Ser Ala Thr 
        115                 120                 125             


Lys Arg Pro Glu Lys Val Ile Gly Met His Phe Phe Asn Pro Val Pro 
    130                 135                 140                 


Val Met Lys Leu Val Glu Val Ile Lys Gly Leu Lys Thr Ser Glu Gln 
145                 150                 155                 160 


Thr Phe Asn Val Val Arg Glu Leu Ala Leu Lys Val Asp Lys Thr Pro 
                165                 170                 175     


Ile Glu Val Lys Glu Ala Pro Gly Phe Val Val Asn Arg Ile Leu Ile 
            180                 185                 190         


Pro Met Ile Asn Glu Ala Ile Gly Ile Leu Ala Asp Gly Val Ala Thr 
        195                 200                 205             


Ala Glu Asp Ile Asp Glu Ala Met Lys Leu Gly Ala Asn His Pro Ile 
    210                 215                 220                 


Gly Pro Leu Ala Leu Ser Asp Leu Ile Gly Asn Asp Val Val Leu Ala 
225                 230                 235                 240 


Ile Met Asn Val Leu Tyr Glu Glu Tyr Gly Asp Ser Lys Tyr Arg Pro 
                245                 250                 255     


His Pro Leu Leu Lys Lys Met Val Arg Gly Gly Leu Leu Gly Arg Lys 
            260                 265                 270         


Thr Gly Lys Gly Phe Phe Glu Tyr Lys 
        275                 280     


<210>  5
<211>  780
<212>  DNA
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  5
atggatttta ataatgtttt attaaataag gatgatggga tagctctcat cattataaat       60

cgtccaaagg ctttaaatgc attaaactat gagacactaa aagagttaga tagtgtgctt      120

gatatagttg aaaatgataa agagataaaa gttttaatta taactggcag cggtgaaaaa      180

gccttcgttg caggtgctga tatagctgag atgagtaata tgacaccact tgaagcgaag      240

aagttctctc tttatggaca gaaagtattt aggaagatag aaatgctaag taagcctgtt      300

atagcagcgg taaatggttt tgcacttggt ggtggatgcg agctttctat ggcatgtgac      360

atacgtattg caagtaaaaa tgcaaaattt ggtcaacctg aagtaggact tggaataata      420

cctggctttt caggaactca aagattacca cgtcttatag gcacttctaa agctaaagag      480

cttattttca caggtgacat gataaattct gatgaagcat ataaaatagg ccttatatct      540

aaagttgttg aactatctga tctcattgaa gaagcaaaaa aactcgcgaa aaaaatgatg      600

tcaaaaagtc aaatagcaat ttctctagca aaggaagcaa taaataaggg aatggaaaca      660

gacttagata caggcaatac tatagaagct gagaaatttt ccttatgttt tacaacagat      720

gatcaaaaag aaggtatgat tgcgttttct gaaaagaggg cgcctaaatt tggcaaataa      780


<210>  6
<211>  259
<212>  PRT
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  6

Met Asp Phe Asn Asn Val Leu Leu Asn Lys Asp Asp Gly Ile Ala Leu 
1               5                   10                  15      


Ile Ile Ile Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Tyr Glu Thr 
            20                  25                  30          


Leu Lys Glu Leu Asp Ser Val Leu Asp Ile Val Glu Asn Asp Lys Glu 
        35                  40                  45              


Ile Lys Val Leu Ile Ile Thr Gly Ser Gly Glu Lys Ala Phe Val Ala 
    50                  55                  60                  


Gly Ala Asp Ile Ala Glu Met Ser Asn Met Thr Pro Leu Glu Ala Lys 
65                  70                  75                  80  


Lys Phe Ser Leu Tyr Gly Gln Lys Val Phe Arg Lys Ile Glu Met Leu 
                85                  90                  95      


Ser Lys Pro Val Ile Ala Ala Val Asn Gly Phe Ala Leu Gly Gly Gly 
            100                 105                 110         


Cys Glu Leu Ser Met Ala Cys Asp Ile Arg Ile Ala Ser Lys Asn Ala 
        115                 120                 125             


Lys Phe Gly Gln Pro Glu Val Gly Leu Gly Ile Ile Pro Gly Phe Ser 
    130                 135                 140                 


Gly Thr Gln Arg Leu Pro Arg Leu Ile Gly Thr Ser Lys Ala Lys Glu 
145                 150                 155                 160 


Leu Ile Phe Thr Gly Asp Met Ile Asn Ser Asp Glu Ala Tyr Lys Ile 
                165                 170                 175     


Gly Leu Ile Ser Lys Val Val Glu Leu Ser Asp Leu Ile Glu Glu Ala 
            180                 185                 190         


Lys Lys Leu Ala Lys Lys Met Met Ser Lys Ser Gln Ile Ala Ile Ser 
        195                 200                 205             


Leu Ala Lys Glu Ala Ile Asn Lys Gly Met Glu Thr Asp Leu Asp Thr 
    210                 215                 220                 


Gly Asn Thr Ile Glu Ala Glu Lys Phe Ser Leu Cys Phe Thr Thr Asp 
225                 230                 235                 240 


Asp Gln Lys Glu Gly Met Ile Ala Phe Ser Glu Lys Arg Ala Pro Lys 
                245                 250                 255     


Phe Gly Lys 
            


<210>  7
<211>  1143
<212>  DNA
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  7
atggactttt cattaacaaa ggagcaagaa atggtaaggc gtgttgtgag agaattcgct       60

gaaaaagaag ttgctcctaa agcaaaagaa atagatatca cagaagagtt tccatgggat      120

acagtaagaa aaatggctca aaacgatatg atgggtattc cttatccaga agagtatggt      180

ggagcaggtg gagattactt gagttatatc atagctgttg aagagatatc aagagcttgt      240

gctacgactg gagtaatttt atctgctcat acttcattgg gaagttttcc aatatatcaa      300

tggggaacag aagaacaaaa aagaaaatat ctagtgccac ttgcaaaagg tgaaaaattg      360

ggcgcttttg gccttacaga acctaacgca ggtacagatg cagctggaca gcagacaact      420

gcagtattag atggtgatca ctacgtatta aacggctcaa aaatatttat tacaaacgga      480

ggaaaagctg acatatatat aatctttgca atgacagaca aatcaaaagg cacaagaggc      540

attagtgcat ttatagttga gaaagatttt ccgggtttta gcattggcaa aattgaagaa      600

aaaatgggta taagagcttc atcaactgcc gaacttgtgt ttgaagattg tattgtacca      660

aaagaaaatt tacttggtaa agaaggagaa ggttttaaaa ttgcgatggc tacactagat      720

ggtggaagaa taggaatagc agcgcaagcc cttggaatag ctcaggctgc tttagatgaa      780

gagataaaat atgcaaagga aagacaacag tttggaagac caattggaaa atttcaaggc      840

attcaatggt atatagctga tatggcaacg agaataaatg cttcaagatg gcttgtatac      900

aatgccgctt ggagaaagca ggtaggtctt ccgtacacaa tggaagcagc tatggcaaaa      960

ttatatgctt ccgaaacagc aatgtttgta acgacaaaaa cagttcagat atttggcggc     1020

tatggcttta caaaagatta tccagtggaa agatttatga gagatgcaaa aataacagaa     1080

atttatgaag gcacatcgga agtccagaaa atggttattt ccggtaacct attgaaaatg     1140

taa                                                                   1143


<210>  8
<211>  380
<212>  PRT
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  8

Met Asp Phe Ser Leu Thr Lys Glu Gln Glu Met Val Arg Arg Val Val 
1               5                   10                  15      


Arg Glu Phe Ala Glu Lys Glu Val Ala Pro Lys Ala Lys Glu Ile Asp 
            20                  25                  30          


Ile Thr Glu Glu Phe Pro Trp Asp Thr Val Arg Lys Met Ala Gln Asn 
        35                  40                  45              


Asp Met Met Gly Ile Pro Tyr Pro Glu Glu Tyr Gly Gly Ala Gly Gly 
    50                  55                  60                  


Asp Tyr Leu Ser Tyr Ile Ile Ala Val Glu Glu Ile Ser Arg Ala Cys 
65                  70                  75                  80  


Ala Thr Thr Gly Val Ile Leu Ser Ala His Thr Ser Leu Gly Ser Phe 
                85                  90                  95      


Pro Ile Tyr Gln Trp Gly Thr Glu Glu Gln Lys Arg Lys Tyr Leu Val 
            100                 105                 110         


Pro Leu Ala Lys Gly Glu Lys Leu Gly Ala Phe Gly Leu Thr Glu Pro 
        115                 120                 125             


Asn Ala Gly Thr Asp Ala Ala Gly Gln Gln Thr Thr Ala Val Leu Asp 
    130                 135                 140                 


Gly Asp His Tyr Val Leu Asn Gly Ser Lys Ile Phe Ile Thr Asn Gly 
145                 150                 155                 160 


Gly Lys Ala Asp Ile Tyr Ile Ile Phe Ala Met Thr Asp Lys Ser Lys 
                165                 170                 175     


Gly Thr Arg Gly Ile Ser Ala Phe Ile Val Glu Lys Asp Phe Pro Gly 
            180                 185                 190         


Phe Ser Ile Gly Lys Ile Glu Glu Lys Met Gly Ile Arg Ala Ser Ser 
        195                 200                 205             


Thr Ala Glu Leu Val Phe Glu Asp Cys Ile Val Pro Lys Glu Asn Leu 
    210                 215                 220                 


Leu Gly Lys Glu Gly Glu Gly Phe Lys Ile Ala Met Ala Thr Leu Asp 
225                 230                 235                 240 


Gly Gly Arg Ile Gly Ile Ala Ala Gln Ala Leu Gly Ile Ala Gln Ala 
                245                 250                 255     


Ala Leu Asp Glu Glu Ile Lys Tyr Ala Lys Glu Arg Gln Gln Phe Gly 
            260                 265                 270         


Arg Pro Ile Gly Lys Phe Gln Gly Ile Gln Trp Tyr Ile Ala Asp Met 
        275                 280                 285             


Ala Thr Arg Ile Asn Ala Ser Arg Trp Leu Val Tyr Asn Ala Ala Trp 
    290                 295                 300                 


Arg Lys Gln Val Gly Leu Pro Tyr Thr Met Glu Ala Ala Met Ala Lys 
305                 310                 315                 320 


Leu Tyr Ala Ser Glu Thr Ala Met Phe Val Thr Thr Lys Thr Val Gln 
                325                 330                 335     


Ile Phe Gly Gly Tyr Gly Phe Thr Lys Asp Tyr Pro Val Glu Arg Phe 
            340                 345                 350         


Met Arg Asp Ala Lys Ile Thr Glu Ile Tyr Glu Gly Thr Ser Glu Val 
        355                 360                 365             


Gln Lys Met Val Ile Ser Gly Asn Leu Leu Lys Met 
    370                 375                 380 


<210>  9
<211>  993
<212>  DNA
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  9
atgaacgatt ataaagatat atggattttt gcagaacaaa gaaatggaaa acttatgaat       60

gttgccatcg agatattagg tgaggcaaga aagcttgcag acaagaaagg cgttaatgtt      120

ggagctgttt taatagggca caatgtagaa aatctttcaa aagatcttat aagttttggc      180

gctgacatag tttatgttgt agacaatcct cttctttcaa attacacaac cgagggctat      240

gcaaaggcaa tatctgagct tgcaaaagag tataaaccag aagtcatact ctatggagca      300

acttttatag gtcgtgatct tgcaccgagg atagcatcta gacttatgac agggcttact      360

gcagattgta cagggctaga tattgacgaa aatggactgc tactgcaaac aaggccggca      420

tttggaggaa acttaatggc aacaataaaa tgtccagaca aaagaccaca gatgtcgaca      480

gtaaggcctg gagtcatgaa aagagctatt agagatgata caagagatgg aaaagtaatt      540

aaatttgatg ctgatattaa tgaaagtgat ataagaacta agatattgag catagcaaaa      600

gaagcaaaaa atgtagtgaa ccttgaagaa gctgacatta tcgtttcagg cggacgtgga      660

attggaggac ctgatggatt taacatcata aaagaacttg cagacgtact gggaggcgtg      720

gttggcgcat ctcgtgcaac agttgatgct gggtggataa catcagacca tcaagttggc      780

cagacaggta agacagttag accaaagctg tacattgctt gcggaattag tggtgcaata      840

cagcatttag caggtatgag caactcaggt actattgtcg caataaataa aaatcctgac      900

gcgccgattt tcaaatttgc tgattacggc atagtcgggg atttattcaa agtaatacca      960

gtattaatag aggaaatcaa aaagttaaaa taa                                   993


<210>  10
<211>  330
<212>  PRT
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  10

Met Asn Asp Tyr Lys Asp Ile Trp Ile Phe Ala Glu Gln Arg Asn Gly 
1               5                   10                  15      


Lys Leu Met Asn Val Ala Ile Glu Ile Leu Gly Glu Ala Arg Lys Leu 
            20                  25                  30          


Ala Asp Lys Lys Gly Val Asn Val Gly Ala Val Leu Ile Gly His Asn 
        35                  40                  45              


Val Glu Asn Leu Ser Lys Asp Leu Ile Ser Phe Gly Ala Asp Ile Val 
    50                  55                  60                  


Tyr Val Val Asp Asn Pro Leu Leu Ser Asn Tyr Thr Thr Glu Gly Tyr 
65                  70                  75                  80  


Ala Lys Ala Ile Ser Glu Leu Ala Lys Glu Tyr Lys Pro Glu Val Ile 
                85                  90                  95      


Leu Tyr Gly Ala Thr Phe Ile Gly Arg Asp Leu Ala Pro Arg Ile Ala 
            100                 105                 110         


Ser Arg Leu Met Thr Gly Leu Thr Ala Asp Cys Thr Gly Leu Asp Ile 
        115                 120                 125             


Asp Glu Asn Gly Leu Leu Leu Gln Thr Arg Pro Ala Phe Gly Gly Asn 
    130                 135                 140                 


Leu Met Ala Thr Ile Lys Cys Pro Asp Lys Arg Pro Gln Met Ser Thr 
145                 150                 155                 160 


Val Arg Pro Gly Val Met Lys Arg Ala Ile Arg Asp Asp Thr Arg Asp 
                165                 170                 175     


Gly Lys Val Ile Lys Phe Asp Ala Asp Ile Asn Glu Ser Asp Ile Arg 
            180                 185                 190         


Thr Lys Ile Leu Ser Ile Ala Lys Glu Ala Lys Asn Val Val Asn Leu 
        195                 200                 205             


Glu Glu Ala Asp Ile Ile Val Ser Gly Gly Arg Gly Ile Gly Gly Pro 
    210                 215                 220                 


Asp Gly Phe Asn Ile Ile Lys Glu Leu Ala Asp Val Leu Gly Gly Val 
225                 230                 235                 240 


Val Gly Ala Ser Arg Ala Thr Val Asp Ala Gly Trp Ile Thr Ser Asp 
                245                 250                 255     


His Gln Val Gly Gln Thr Gly Lys Thr Val Arg Pro Lys Leu Tyr Ile 
            260                 265                 270         


Ala Cys Gly Ile Ser Gly Ala Ile Gln His Leu Ala Gly Met Ser Asn 
        275                 280                 285             


Ser Gly Thr Ile Val Ala Ile Asn Lys Asn Pro Asp Ala Pro Ile Phe 
    290                 295                 300                 


Lys Phe Ala Asp Tyr Gly Ile Val Gly Asp Leu Phe Lys Val Ile Pro 
305                 310                 315                 320 


Val Leu Ile Glu Glu Ile Lys Lys Leu Lys 
                325                 330 


<210>  11
<211>  783
<212>  DNA
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  11
atgaatatac ttgttttgat taagcaggtt ccggatacga acgaagttag aattgatcct       60

gtaacaaaga cacttatacg tgaaggtgtt ccaagcatta ttaatcctga tgacaaaaat      120

gccttagaag aagctatcag gataagagaa aaagtcggcg gaaaagtaac tgtaatatcg      180

atgggaccaa cacaagcaga agtagcatta cgtgaggctc ttgctatggg tgctgatgaa      240

gcatatcttt tgacagatag agcatttgca ggcgcagaca cttatgctac tgcaaaagca      300

ctatcaaaag caattgaaaa gtttcagtac gacattgtat tttgcggcag acaggcgata      360

gatggcgata ctgcacaagt tggaccacaa attgcagaac aattagacat acctcaggta      420

acatatgtaa ggaaggtaga aatagaagga gacaaactca tagttgaaag agctcttgaa      480

gatggatatg aaatcattga agttaagacg ccagtcttat tgacagctat taaagaactt      540

aatgtgccta ggtatccttc aataaaagga atattcaatg cttacaataa aaaagaagtt      600

aaaatcttaa ctgcagatga tttagaagtt gataaaaatg aacttggact aaaaggttca      660

ccaactaagg ttgtagcaac gtcaactcca aatactgaaa gagctggaga aatatttact      720

ggcaatataa aagaagctgt tcagaatcta gttgaaagat taaacagcag acatgtgata      780

tag                                                                    783


<210>  12
<211>  260
<212>  PRT
<213>  Thermoanaerobacterium thermosaccharolyticum

<400>  12

Met Asn Ile Leu Val Leu Ile Lys Gln Val Pro Asp Thr Asn Glu Val 
1               5                   10                  15      


Arg Ile Asp Pro Val Thr Lys Thr Leu Ile Arg Glu Gly Val Pro Ser 
            20                  25                  30          


Ile Ile Asn Pro Asp Asp Lys Asn Ala Leu Glu Glu Ala Ile Arg Ile 
        35                  40                  45              


Arg Glu Lys Val Gly Gly Lys Val Thr Val Ile Ser Met Gly Pro Thr 
    50                  55                  60                  


Gln Ala Glu Val Ala Leu Arg Glu Ala Leu Ala Met Gly Ala Asp Glu 
65                  70                  75                  80  


Ala Tyr Leu Leu Thr Asp Arg Ala Phe Ala Gly Ala Asp Thr Tyr Ala 
                85                  90                  95      


Thr Ala Lys Ala Leu Ser Lys Ala Ile Glu Lys Phe Gln Tyr Asp Ile 
            100                 105                 110         


Val Phe Cys Gly Arg Gln Ala Ile Asp Gly Asp Thr Ala Gln Val Gly 
        115                 120                 125             


Pro Gln Ile Ala Glu Gln Leu Asp Ile Pro Gln Val Thr Tyr Val Arg 
    130                 135                 140                 


Lys Val Glu Ile Glu Gly Asp Lys Leu Ile Val Glu Arg Ala Leu Glu 
145                 150                 155                 160 


Asp Gly Tyr Glu Ile Ile Glu Val Lys Thr Pro Val Leu Leu Thr Ala 
                165                 170                 175     


Ile Lys Glu Leu Asn Val Pro Arg Tyr Pro Ser Ile Lys Gly Ile Phe 
            180                 185                 190         


Asn Ala Tyr Asn Lys Lys Glu Val Lys Ile Leu Thr Ala Asp Asp Leu 
        195                 200                 205             


Glu Val Asp Lys Asn Glu Leu Gly Leu Lys Gly Ser Pro Thr Lys Val 
    210                 215                 220                 


Val Ala Thr Ser Thr Pro Asn Thr Glu Arg Ala Gly Glu Ile Phe Thr 
225                 230                 235                 240 


Gly Asn Ile Lys Glu Ala Val Gln Asn Leu Val Glu Arg Leu Asn Ser 
                245                 250                 255     


Arg His Val Ile 
            260 


<210>  13
<211>  2589
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  13
atgaaagtca caacagtaaa ggaattagat gaaaaactca aggtaattaa agaagctcaa       60

aaaaaattct cttgttactc gcaagaaatg gttgatgaaa tctttagaaa tgcagcaatg      120

gcagcaatcg acgcaaggat agagctagca aaagcagctg ttttggaaac cggtatgggc      180

ttagttgaag acaaggttat aaaaaatcat tttgcaggcg aatacatcta taacaaatat      240

aaggatgaaa aaacctgcgg tataattgaa cgaaatgaac cctacggaat tacaaaaata      300

gcagaaccta taggagttgt agctgctata atccctgtaa caaaccccac atcaacaaca      360

atatttaaat ccttaatatc ccttaaaact agaaatggaa ttttcttttc gcctcaccca      420

agggcaaaaa aatccacaat actagcagct aaaacaatac ttgatgcagc cgttaagagt      480

ggtgccccgg aaaatataat aggttggata gatgaacctt caattgaact aactcaatat      540

ttaatgcaaa aagcagatat aacccttgca actggtggtc cctcactagt taaatctgct      600

tattcttccg gaaaaccagc aataggtgtt ggtccgggta acaccccagt aataattgat      660

gaatctgctc atataaaaat ggcagtaagt tcaattatat tatccaaaac ctatgataat      720

ggtgttatat gtgcttctga acaatctgta atagtcttaa aatccatata taacaaggta      780

aaagatgagt tccaagaaag aggagcttat ataataaaga aaaacgaatt ggataaagtc      840

cgtgaagtga tttttaaaga tggatccgta aaccctaaaa tagtcggaca gtcagcttat      900

actatagcag ctatggctgg cataaaagta cctaaaacca caagaatatt aataggagaa      960

gttacctcct taggtgaaga agaacctttt gcccacgaaa aactatctcc tgttttggct     1020

atgtatgagg ctgacaattt tgatgatgct ttaaaaaaag cagtaactct aataaactta     1080

ggaggcctcg gccatacctc aggaatatat gcagatgaaa taaaagcacg agataaaata     1140

gatagattta gtagtgccat gaaaaccgta agaacctttg taaatatccc aacctcacaa     1200

ggtgcaagtg gagatctata taattttaga ataccacctt ctttcacgct tggctgcgga     1260

ttttggggag gaaattctgt ttccgagaat gttggtccaa aacatctttt gaatattaaa     1320

accgtagctg aaaggagaga aaacatgctt tggtttagag ttccacataa agtatatttt     1380

aagttcggtt gtcttcaatt tgctttaaaa gatttaaaag atctaaagaa aaaaagagcc     1440

tttatagtta ctgatagtga cccctataat ttaaactatg ttgattcaat aataaaaata     1500

cttgagcacc tagatattga ttttaaagta tttaataagg ttggaagaga agctgatctt     1560

aaaaccataa aaaaagcaac tgaagaaatg tcctccttta tgccagacac tataatagct     1620

ttaggtggta cccctgaaat gagctctgca aagctaatgt gggtactata tgaacatcca     1680

gaagtaaaat ttgaagatct tgcaataaaa tttatggaca taagaaagag aatatatact     1740

ttcccaaaac tcggtaaaaa ggctatgtta gttgcaatta caacttctgc tggttccggt     1800

tctgaggtta ctccttttgc tttagtaact gacaataaca ctggaaataa gtacatgtta     1860

gcagattatg aaatgacacc aaatatggca attgtagatg cagaacttat gatgaaaatg     1920

ccaaagggat taaccgctta ttcaggtata gatgcactag taaatagtat agaagcatac     1980

acatccgtat atgcttcaga atacacaaac ggactagcac tagaggcaat acgattaata     2040

tttaaatatt tgcctgaggc ttacaaaaac ggaagaacca atgaaaaagc aagagagaaa     2100

atggctcacg cttcaactat ggcaggtatg gcatccgcta atgcatttct aggtctatgt     2160

cattccatgg caataaaatt aagttcagaa cacaatattc ctagtggcat tgccaatgca     2220

ttactaatag aagaagtaat aaaatttaac gcagttgata atcctgtaaa acaagcccct     2280

tgcccacaat ataagtatcc aaacaccata tttagatatg ctcgaattgc agattatata     2340

aagcttggag gaaatactga tgaggaaaag gtagatctct taattaacaa aatacatgaa     2400

ctaaaaaaag ctttaaatat accaacttca ataaaggatg caggtgtttt ggaggaaaac     2460

ttctattcct cccttgatag aatatctgaa cttgcactag atgatcaatg cacaggcgct     2520

aatcctagat ttcctcttac aagtgagata aaagaaatgt atataaattg ttttaaaaaa     2580

caaccttaa                                                             2589


<210>  14
<211>  862
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  14

Met Lys Val Thr Thr Val Lys Glu Leu Asp Glu Lys Leu Lys Val Ile 
1               5                   10                  15      


Lys Glu Ala Gln Lys Lys Phe Ser Cys Tyr Ser Gln Glu Met Val Asp 
            20                  25                  30          


Glu Ile Phe Arg Asn Ala Ala Met Ala Ala Ile Asp Ala Arg Ile Glu 
        35                  40                  45              


Leu Ala Lys Ala Ala Val Leu Glu Thr Gly Met Gly Leu Val Glu Asp 
    50                  55                  60                  


Lys Val Ile Lys Asn His Phe Ala Gly Glu Tyr Ile Tyr Asn Lys Tyr 
65                  70                  75                  80  


Lys Asp Glu Lys Thr Cys Gly Ile Ile Glu Arg Asn Glu Pro Tyr Gly 
                85                  90                  95      


Ile Thr Lys Ile Ala Glu Pro Ile Gly Val Val Ala Ala Ile Ile Pro 
            100                 105                 110         


Val Thr Asn Pro Thr Ser Thr Thr Ile Phe Lys Ser Leu Ile Ser Leu 
        115                 120                 125             


Lys Thr Arg Asn Gly Ile Phe Phe Ser Pro His Pro Arg Ala Lys Lys 
    130                 135                 140                 


Ser Thr Ile Leu Ala Ala Lys Thr Ile Leu Asp Ala Ala Val Lys Ser 
145                 150                 155                 160 


Gly Ala Pro Glu Asn Ile Ile Gly Trp Ile Asp Glu Pro Ser Ile Glu 
                165                 170                 175     


Leu Thr Gln Tyr Leu Met Gln Lys Ala Asp Ile Thr Leu Ala Thr Gly 
            180                 185                 190         


Gly Pro Ser Leu Val Lys Ser Ala Tyr Ser Ser Gly Lys Pro Ala Ile 
        195                 200                 205             


Gly Val Gly Pro Gly Asn Thr Pro Val Ile Ile Asp Glu Ser Ala His 
    210                 215                 220                 


Ile Lys Met Ala Val Ser Ser Ile Ile Leu Ser Lys Thr Tyr Asp Asn 
225                 230                 235                 240 


Gly Val Ile Cys Ala Ser Glu Gln Ser Val Ile Val Leu Lys Ser Ile 
                245                 250                 255     


Tyr Asn Lys Val Lys Asp Glu Phe Gln Glu Arg Gly Ala Tyr Ile Ile 
            260                 265                 270         


Lys Lys Asn Glu Leu Asp Lys Val Arg Glu Val Ile Phe Lys Asp Gly 
        275                 280                 285             


Ser Val Asn Pro Lys Ile Val Gly Gln Ser Ala Tyr Thr Ile Ala Ala 
    290                 295                 300                 


Met Ala Gly Ile Lys Val Pro Lys Thr Thr Arg Ile Leu Ile Gly Glu 
305                 310                 315                 320 


Val Thr Ser Leu Gly Glu Glu Glu Pro Phe Ala His Glu Lys Leu Ser 
                325                 330                 335     


Pro Val Leu Ala Met Tyr Glu Ala Asp Asn Phe Asp Asp Ala Leu Lys 
            340                 345                 350         


Lys Ala Val Thr Leu Ile Asn Leu Gly Gly Leu Gly His Thr Ser Gly 
        355                 360                 365             


Ile Tyr Ala Asp Glu Ile Lys Ala Arg Asp Lys Ile Asp Arg Phe Ser 
    370                 375                 380                 


Ser Ala Met Lys Thr Val Arg Thr Phe Val Asn Ile Pro Thr Ser Gln 
385                 390                 395                 400 


Gly Ala Ser Gly Asp Leu Tyr Asn Phe Arg Ile Pro Pro Ser Phe Thr 
                405                 410                 415     


Leu Gly Cys Gly Phe Trp Gly Gly Asn Ser Val Ser Glu Asn Val Gly 
            420                 425                 430         


Pro Lys His Leu Leu Asn Ile Lys Thr Val Ala Glu Arg Arg Glu Asn 
        435                 440                 445             


Met Leu Trp Phe Arg Val Pro His Lys Val Tyr Phe Lys Phe Gly Cys 
    450                 455                 460                 


Leu Gln Phe Ala Leu Lys Asp Leu Lys Asp Leu Lys Lys Lys Arg Ala 
465                 470                 475                 480 


Phe Ile Val Thr Asp Ser Asp Pro Tyr Asn Leu Asn Tyr Val Asp Ser 
                485                 490                 495     


Ile Ile Lys Ile Leu Glu His Leu Asp Ile Asp Phe Lys Val Phe Asn 
            500                 505                 510         


Lys Val Gly Arg Glu Ala Asp Leu Lys Thr Ile Lys Lys Ala Thr Glu 
        515                 520                 525             


Glu Met Ser Ser Phe Met Pro Asp Thr Ile Ile Ala Leu Gly Gly Thr 
    530                 535                 540                 


Pro Glu Met Ser Ser Ala Lys Leu Met Trp Val Leu Tyr Glu His Pro 
545                 550                 555                 560 


Glu Val Lys Phe Glu Asp Leu Ala Ile Lys Phe Met Asp Ile Arg Lys 
                565                 570                 575     


Arg Ile Tyr Thr Phe Pro Lys Leu Gly Lys Lys Ala Met Leu Val Ala 
            580                 585                 590         


Ile Thr Thr Ser Ala Gly Ser Gly Ser Glu Val Thr Pro Phe Ala Leu 
        595                 600                 605             


Val Thr Asp Asn Asn Thr Gly Asn Lys Tyr Met Leu Ala Asp Tyr Glu 
    610                 615                 620                 


Met Thr Pro Asn Met Ala Ile Val Asp Ala Glu Leu Met Met Lys Met 
625                 630                 635                 640 


Pro Lys Gly Leu Thr Ala Tyr Ser Gly Ile Asp Ala Leu Val Asn Ser 
                645                 650                 655     


Ile Glu Ala Tyr Thr Ser Val Tyr Ala Ser Glu Tyr Thr Asn Gly Leu 
            660                 665                 670         


Ala Leu Glu Ala Ile Arg Leu Ile Phe Lys Tyr Leu Pro Glu Ala Tyr 
        675                 680                 685             


Lys Asn Gly Arg Thr Asn Glu Lys Ala Arg Glu Lys Met Ala His Ala 
    690                 695                 700                 


Ser Thr Met Ala Gly Met Ala Ser Ala Asn Ala Phe Leu Gly Leu Cys 
705                 710                 715                 720 


His Ser Met Ala Ile Lys Leu Ser Ser Glu His Asn Ile Pro Ser Gly 
                725                 730                 735     


Ile Ala Asn Ala Leu Leu Ile Glu Glu Val Ile Lys Phe Asn Ala Val 
            740                 745                 750         


Asp Asn Pro Val Lys Gln Ala Pro Cys Pro Gln Tyr Lys Tyr Pro Asn 
        755                 760                 765             


Thr Ile Phe Arg Tyr Ala Arg Ile Ala Asp Tyr Ile Lys Leu Gly Gly 
    770                 775                 780                 


Asn Thr Asp Glu Glu Lys Val Asp Leu Leu Ile Asn Lys Ile His Glu 
785                 790                 795                 800 


Leu Lys Lys Ala Leu Asn Ile Pro Thr Ser Ile Lys Asp Ala Gly Val 
                805                 810                 815     


Leu Glu Glu Asn Phe Tyr Ser Ser Leu Asp Arg Ile Ser Glu Leu Ala 
            820                 825                 830         


Leu Asp Asp Gln Cys Thr Gly Ala Asn Pro Arg Phe Pro Leu Thr Ser 
        835                 840                 845             


Glu Ile Lys Glu Met Tyr Ile Asn Cys Phe Lys Lys Gln Pro 
    850                 855                 860         


