                               SEQUENCE LISTING

<110> UNIVERSITY OF IOWA RESEARCH FOUNDATION
 
<120> OLIGONUCLEOTIDE-BASED PROBES FOR DETECTION OF BACTERIAL NUCLEASES

<130> 17025.003WO1

<140> PCT/US2012/053195
<141> 2012-08-30

<150> 61/593,595
<151> 2012-02-01

<150> 61/530,246
<151> 2011-09-01

<160> 18    

<170> PatentIn version 3.5

<210> 1
<211> 10
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<223> 5'-56-FAM

<220>
<223> 3'-ZEN/3IAbRQSp

<400> 1
ttccttcctc                                                              10


<210> 2
<211> 12
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<223> 5'-56-FAM

<220>
<221> modified_base
<222> (1)..(12)
<223> 2'-O-methyl nucleotide

<220>
<223> 3'-ZEN/3IAbRQSp

<400> 2
ucucguacgu uc                                                           12


<210> 3
<211> 12
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<223> 5'-56-FAM

<220>
<221> modified_base
<222> (1)..(4)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (6)..(6)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (8)..(8)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (10)..(12)
<223> 2'-fluoro nucleotide

<220>
<223> 3'-ZEN/3IAbRQSp

<400> 3
ucucguacgu uc                                                           12


<210> 4
<211> 11
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide


<220>
<223> 5'-56-FAM

<220>
<221> modified_base
<222> (1)..(4)
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (7)..(11)
<223> 2'-O-methyl nucleotide

<220>
<223> 3'-ZEN/3IAbRQSp

<400> 4
cucgaacguu c                                                            11


<210> 5
<211> 11
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide


<220>
<223> 5'-56-FAM

<220>
<221> modified_base
<222> (1)..(4)
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (7)..(11)
<223> 2'-O-methyl nucleotide

<220>
<223> 3'-ZEN/3IAbRQSp

<400> 5
cucgttcguu c                                                            11


<210> 6
<211> 11
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide


<220>
<223> 5'-56-FAM

<220>
<221> modified_base
<222> (1)..(4)
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (7)..(11)
<223> 2'-O-methyl nucleotide

<220>
<223> 3'-ZEN/3IAbRQSp

<400> 6
cucgatcguu c                                                            11


<210> 7
<211> 12
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 7
ucucguacgu uc                                                           12


<210> 8
<211> 51
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 8
gggaggacga ugcgggacua gcgaucuguu acgcacagac gacucgcccg a                51


<210> 9
<211> 51
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (8)..(8)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (11)..(11)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (13)..(13)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (18)..(19)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (22)..(22)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (25)..(27)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (29)..(30)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (32)..(32)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (34)..(34)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (36)..(36)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (40)..(40)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (43)..(45)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (47)..(49)
<223> 2'-fluoro nucleotide

<220>
<223> 3'-FAM

<400> 9
gggaggacga ugcgggacua gcgaucuguu acgcacagac gacucgcccg a                51


<210> 10
<211> 12
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      probe


<220>
<223> 5'-FAM

<220>
<221> modified_base
<222> (1)..(4)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (6)..(6)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (8)..(8)
<223> 2'-fluoro nucleotide

<220>
<221> modified_base
<222> (10)..(12)
<223> 2'-fluoro nucleotide

<220>
<223> 3'-ZEN-RQ

<400> 10
ucucguacgu uc                                                           12


<210> 11
<211> 12
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      probe 


<220>
<223> 5'-FAM

<220>
<221> modified_base
<222> (1)..(4)
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (6)..(6)
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (8)..(8)
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (10)..(12)
<223> 2'-O-methyl nucleotide

<220>
<223> 3'-ZEN-RQ

<400> 11
ucucguacgu uc                                                           12


<210> 12
<211> 12
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      probe 


<220>
<223> 5'-FAM

<220>
<221> modified_base
<222> (1)..(12)
<223> 2'-fluoro nucleotide

<220>
<223> 3'-ZEN-RQ

<400> 12
ucucguacgu uc                                                           12


<210> 13
<211> 12
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      probe 


<220>
<223> 5'-FAM

<220>
<221> modified_base
<222> (1)..(12)
<223> 2'-O-methyl nucleotide

<220>
<223> 3'-ZEN-RQ

<400> 13
ucucguacgu uc                                                           12


<210> 14
<211> 10
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      probe 


<220>
<223> 5'-FAM

<220>
<223> 3'-ZEN-RQ

<400> 14
ttccttcctc                                                              10


<210> 15
<211> 11
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      probe

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      probe


<220>
<223> 5'-FAM

<220>
<221> modified_base
<222> (1)..(4)
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (7)..(11)
<223> 2'-O-methyl nucleotide

<220>
<223> 3'-ZEN-RQ

<400> 15
cucgttcguu c                                                            11


<210> 16
<211> 11
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      probe

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      probe


<220>
<223> 5'-Cy5.5

<220>
<221> modified_base
<222> (1)..(4)
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (7)..(11)
<223> 2'-O-methyl nucleotide

<220>
<223> 3'-ZEN-RQ

<400> 16
cucgttcguu c                                                            11


<210> 17
<211> 12
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      probe

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      probe


<220>
<223> 5'-Cy5.5

<220>
<221> modified_base
<222> (1)..(4)
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (7)..(11)
<223> 2'-O-methyl nucleotide

<220>
<221> modified_base
<222> (12)..(12)
<223> inverted dT

<220>
<223> 3'-ZEN-RQ

<400> 17
cucgttcguu ct                                                           12


<210> 18
<211> 11
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 18
cucguacguu c                                                            11


