                         SEQUENCE LISTING

<110>  INSERM
 
<120>  METHOD FOR PREPARING INDUCED PARAXIAL MESODERM PROGENITOR (IPAM) 
       CELLS AND THEIR USE

<130>  BIO12155 POURQUIE

<160>  11    

<170>  PatentIn version 3.3

<210>  1
<211>  272
<212>  PRT
<213>  Homo sapiens

<400>  1

Met His Leu Arg Leu Ile Ser Trp Leu Phe Ile Ile Leu Asn Phe Met 
1               5                   10                  15      


Glu Tyr Ile Gly Ser Gln Asn Ala Ser Arg Gly Arg Arg Gln Arg Arg 
            20                  25                  30          


Met His Pro Asn Val Ser Gln Gly Cys Gln Gly Gly Cys Ala Thr Cys 
        35                  40                  45              


Ser Asp Tyr Asn Gly Cys Leu Ser Cys Lys Pro Arg Leu Phe Phe Ala 
    50                  55                  60                  


Leu Glu Arg Ile Gly Met Lys Gln Ile Gly Val Cys Leu Ser Ser Cys 
65                  70                  75                  80  


Pro Ser Gly Tyr Tyr Gly Thr Arg Tyr Pro Asp Ile Asn Lys Cys Thr 
                85                  90                  95      


Lys Cys Lys Ala Asp Cys Asp Thr Cys Phe Asn Lys Asn Phe Cys Thr 
            100                 105                 110         


Lys Cys Lys Ser Gly Phe Tyr Leu His Leu Gly Lys Cys Leu Asp Asn 
        115                 120                 125             


Cys Pro Glu Gly Leu Glu Ala Asn Asn His Thr Met Glu Cys Val Ser 
    130                 135                 140                 


Ile Val His Cys Glu Val Ser Glu Trp Asn Pro Trp Ser Pro Cys Thr 
145                 150                 155                 160 


Lys Lys Gly Lys Thr Cys Gly Phe Lys Arg Gly Thr Glu Thr Arg Val 
                165                 170                 175     


Arg Glu Ile Ile Gln His Pro Ser Ala Lys Gly Asn Leu Cys Pro Pro 
            180                 185                 190         


Thr Asn Glu Thr Arg Lys Cys Thr Val Gln Arg Lys Lys Cys Gln Lys 
        195                 200                 205             


Gly Glu Arg Gly Lys Lys Gly Arg Glu Arg Lys Arg Lys Lys Pro Asn 
    210                 215                 220                 


Lys Gly Glu Ser Lys Glu Ala Ile Pro Asp Ser Lys Ser Leu Glu Ser 
225                 230                 235                 240 


Ser Lys Glu Ile Pro Glu Gln Arg Glu Asn Lys Gln Gln Gln Lys Lys 
                245                 250                 255     


Arg Lys Val Gln Asp Lys Gln Lys Ser Val Ser Val Ser Thr Val His 
            260                 265                 270         


<210>  2
<211>  277
<212>  PRT
<213>  Mus musculus

<400>  2

Met His Leu Arg Leu Ile Ser Cys Phe Phe Ile Ile Leu Asn Phe Met 
1               5                   10                  15      


Glu Tyr Ile Gly Ser Gln Asn Ala Ser Arg Gly Arg Arg Gln Arg Arg 
            20                  25                  30          


Met His Pro Asn Val Ser Gln Gly Cys Gln Gly Gly Cys Ala Thr Cys 
        35                  40                  45              


Ser Asp Tyr Asn Gly Cys Leu Ser Cys Lys Pro Arg Leu Phe Phe Val 
    50                  55                  60                  


Leu Glu Arg Ile Gly Met Lys Gln Ile Gly Val Cys Leu Ser Ser Cys 
65                  70                  75                  80  


Pro Ser Gly Tyr Tyr Gly Thr Arg Tyr Pro Asp Ile Asn Lys Cys Thr 
                85                  90                  95      


Lys Cys Lys Val Asp Cys Asp Thr Cys Phe Asn Lys Asn Phe Cys Thr 
            100                 105                 110         


Lys Cys Lys Ser Gly Phe Tyr Leu His Leu Gly Lys Cys Leu Asp Ser 
        115                 120                 125             


Cys Pro Glu Gly Leu Glu Ala Asn Asn His Thr Met Glu Cys Val Ser 
    130                 135                 140                 


Ile Val His Cys Glu Ala Ser Glu Trp Ser Pro Trp Ser Pro Cys Met 
145                 150                 155                 160 


Lys Lys Gly Lys Thr Cys Gly Phe Lys Arg Gly Thr Glu Thr Arg Val 
                165                 170                 175     


Arg Asp Ile Leu Gln His Pro Ser Ala Lys Gly Asn Leu Cys Pro Pro 
            180                 185                 190         


Thr Ser Glu Thr Arg Thr Cys Ile Val Gln Arg Lys Lys Cys Ser Lys 
        195                 200                 205             


Gly Glu Arg Gly Lys Lys Gly Arg Glu Arg Lys Arg Lys Lys Leu Asn 
    210                 215                 220                 


Lys Glu Glu Arg Lys Glu Thr Ser Ser Ser Ser Asp Ser Lys Gly Leu 
225                 230                 235                 240 


Glu Ser Ser Ile Glu Thr Pro Asp Gln Gln Glu Asn Lys Glu Arg Gln 
                245                 250                 255     


Gln Gln Gln Lys Arg Arg Ala Arg Asp Lys Gln Gln Lys Ser Val Ser 
            260                 265                 270         


Val Ser Thr Val His 
        275         


<210>  3
<211>  243
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Gln Phe Arg Leu Phe Ser Phe Ala Leu Ile Ile Leu Asn Cys Met 
1               5                   10                  15      


Asp Tyr Ser His Cys Gln Gly Asn Arg Trp Arg Arg Ser Lys Arg Ala 
            20                  25                  30          


Ser Tyr Val Ser Asn Pro Ile Cys Lys Gly Cys Leu Ser Cys Ser Lys 
        35                  40                  45              


Asp Asn Gly Cys Ser Arg Cys Gln Gln Lys Leu Phe Phe Phe Leu Arg 
    50                  55                  60                  


Arg Glu Gly Met Arg Gln Tyr Gly Glu Cys Leu His Ser Cys Pro Ser 
65                  70                  75                  80  


Gly Tyr Tyr Gly His Arg Ala Pro Asp Met Asn Arg Cys Ala Arg Cys 
                85                  90                  95      


Arg Ile Glu Asn Cys Asp Ser Cys Phe Ser Lys Asp Phe Cys Thr Lys 
            100                 105                 110         


Cys Lys Val Gly Phe Tyr Leu His Arg Gly Arg Cys Phe Asp Glu Cys 
        115                 120                 125             


Pro Asp Gly Phe Ala Pro Leu Glu Glu Thr Met Glu Cys Val Glu Gly 
    130                 135                 140                 


Cys Glu Val Gly His Trp Ser Glu Trp Gly Thr Cys Ser Arg Asn Asn 
145                 150                 155                 160 


Arg Thr Cys Gly Phe Lys Trp Gly Leu Glu Thr Arg Thr Arg Gln Ile 
                165                 170                 175     


Val Lys Lys Pro Val Lys Asp Thr Ile Leu Cys Pro Thr Ile Ala Glu 
            180                 185                 190         


Ser Arg Arg Cys Lys Met Thr Met Arg His Cys Pro Gly Gly Lys Arg 
        195                 200                 205             


Thr Pro Lys Ala Lys Glu Lys Arg Asn Lys Lys Lys Lys Arg Lys Leu 
    210                 215                 220                 


Ile Glu Arg Ala Gln Glu Gln His Ser Val Phe Leu Ala Thr Asp Arg 
225                 230                 235                 240 


Ala Asn Gln 
            


<210>  4
<211>  243
<212>  PRT
<213>  Mus musculus

<400>  4

Met Arg Phe Cys Leu Phe Ser Phe Ala Leu Ile Ile Leu Asn Cys Met 
1               5                   10                  15      


Asp Tyr Ser Gln Cys Gln Gly Asn Arg Trp Arg Arg Asn Lys Arg Ala 
            20                  25                  30          


Ser Tyr Val Ser Asn Pro Ile Cys Lys Gly Cys Leu Ser Cys Ser Lys 
        35                  40                  45              


Asp Asn Gly Cys Ser Arg Cys Gln Gln Lys Leu Phe Phe Phe Leu Arg 
    50                  55                  60                  


Arg Glu Gly Met Arg Gln Tyr Gly Glu Cys Leu His Ser Cys Pro Ser 
65                  70                  75                  80  


Gly Tyr Tyr Gly His Arg Ala Pro Asp Met Asn Arg Cys Ala Arg Cys 
                85                  90                  95      


Arg Ile Glu Asn Cys Asp Ser Cys Phe Ser Lys Asp Phe Cys Thr Lys 
            100                 105                 110         


Cys Lys Val Gly Phe Tyr Leu His Arg Gly Arg Cys Phe Asp Glu Cys 
        115                 120                 125             


Pro Asp Gly Phe Ala Pro Leu Asp Glu Thr Met Glu Cys Val Glu Gly 
    130                 135                 140                 


Cys Glu Val Gly His Trp Ser Glu Trp Gly Thr Cys Ser Arg Asn Asn 
145                 150                 155                 160 


Arg Thr Cys Gly Phe Lys Trp Gly Leu Glu Thr Arg Thr Arg Gln Ile 
                165                 170                 175     


Val Lys Lys Pro Ala Lys Asp Thr Ile Pro Cys Pro Thr Ile Ala Glu 
            180                 185                 190         


Ser Arg Arg Cys Lys Met Ala Met Arg His Cys Pro Gly Gly Lys Arg 
        195                 200                 205             


Thr Pro Lys Ala Lys Glu Lys Arg Asn Lys Lys Lys Arg Arg Lys Leu 
    210                 215                 220                 


Ile Glu Arg Ala Gln Glu Gln His Ser Val Phe Leu Ala Thr Asp Arg 
225                 230                 235                 240 


Val Asn Gln 
            


<210>  5
<211>  292
<212>  PRT
<213>  Homo sapiens

<400>  5

Met His Leu Arg Leu Ile Ser Trp Leu Phe Ile Ile Leu Asn Phe Met 
1               5                   10                  15      


Glu Tyr Ile Gly Ser Gln Asn Ala Ser Arg Gly Arg Arg Gln Arg Arg 
            20                  25                  30          


Met His Pro Asn Val Ser Gln Gly Cys Gln Gly Gly Cys Ala Thr Cys 
        35                  40                  45              


Ser Asp Tyr Asn Gly Cys Leu Ser Cys Lys Pro Arg Leu Phe Phe Ala 
    50                  55                  60                  


Leu Glu Arg Ile Gly Met Lys Gln Ile Gly Val Cys Leu Ser Ser Cys 
65                  70                  75                  80  


Pro Ser Gly Tyr Tyr Gly Thr Arg Tyr Pro Asp Ile Asn Lys Cys Thr 
                85                  90                  95      


Lys Cys Lys Ala Asp Cys Asp Thr Cys Phe Asn Lys Asn Phe Cys Thr 
            100                 105                 110         


Lys Cys Lys Ser Gly Phe Tyr Leu His Leu Gly Lys Cys Leu Asp Asn 
        115                 120                 125             


Cys Pro Glu Gly Leu Glu Ala Asn Asn His Thr Met Glu Cys Val Ser 
    130                 135                 140                 


Ile Val His Cys Glu Val Ser Glu Trp Asn Pro Trp Ser Pro Cys Thr 
145                 150                 155                 160 


Lys Lys Gly Lys Thr Cys Gly Phe Lys Arg Gly Thr Glu Thr Arg Val 
                165                 170                 175     


Arg Glu Ile Ile Gln His Pro Ser Ala Lys Gly Asn Leu Cys Pro Pro 
            180                 185                 190         


Thr Asn Glu Thr Arg Lys Cys Thr Val Gln Arg Lys Lys Cys Gln Lys 
        195                 200                 205             


Gly Glu Arg Gly Lys Lys Gly Arg Glu Arg Lys Arg Lys Lys Pro Asn 
    210                 215                 220                 


Lys Gly Glu Ser Lys Glu Ala Ile Pro Asp Ser Lys Ser Leu Glu Ser 
225                 230                 235                 240 


Ser Lys Glu Ile Pro Glu Gln Arg Glu Asn Lys Gln Gln Gln Lys Lys 
                245                 250                 255     


Arg Lys Val Gln Asp Lys Gln Lys Ser Gly Ile Glu Val Thr Leu Ala 
            260                 265                 270         


Glu Gly Leu Thr Ser Val Ser Gln Arg Thr Gln Pro Thr Pro Cys Arg 
        275                 280                 285             


Arg Arg Tyr Leu 
    290         


<210>  6
<211>  176
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Arg Gln Tyr Gly Glu Cys Leu His Ser Cys Pro Ser Gly Tyr Tyr 
1               5                   10                  15      


Gly His Arg Ala Pro Asp Met Asn Arg Cys Ala Arg Cys Arg Ile Glu 
            20                  25                  30          


Asn Cys Asp Ser Cys Phe Ser Lys Asp Phe Cys Thr Lys Cys Lys Val 
        35                  40                  45              


Gly Phe Tyr Leu His Arg Gly Arg Cys Phe Asp Glu Cys Pro Asp Gly 
    50                  55                  60                  


Phe Ala Pro Leu Glu Glu Thr Met Glu Cys Val Glu Gly Cys Glu Val 
65                  70                  75                  80  


Gly His Trp Ser Glu Trp Gly Thr Cys Ser Arg Asn Asn Arg Thr Cys 
                85                  90                  95      


Gly Phe Lys Trp Gly Leu Glu Thr Arg Thr Arg Gln Ile Val Lys Lys 
            100                 105                 110         


Pro Val Lys Asp Thr Ile Leu Cys Pro Thr Ile Ala Glu Ser Arg Arg 
        115                 120                 125             


Cys Lys Met Thr Met Arg His Cys Pro Gly Gly Lys Arg Thr Pro Lys 
    130                 135                 140                 


Ala Lys Glu Lys Arg Asn Lys Lys Lys Lys Arg Lys Leu Ile Glu Arg 
145                 150                 155                 160 


Ala Gln Glu Gln His Ser Val Phe Leu Ala Thr Asp Arg Ala Asn Gln 
                165                 170                 175     


<210>  7
<211>  177
<212>  PRT
<213>  Homo sapiens

<400>  7

Phe Arg Leu Phe Ser Phe Ala Leu Ile Ile Leu Asn Cys Met Asp Tyr 
1               5                   10                  15      


Ser His Cys Gln Gly Asn Arg Trp Arg Arg Ser Lys Arg Gly Cys Arg 
            20                  25                  30          


Ile Glu Asn Cys Asp Ser Cys Phe Ser Lys Asp Phe Cys Thr Lys Cys 
        35                  40                  45              


Lys Val Gly Phe Tyr Leu His Arg Gly Arg Cys Phe Asp Glu Cys Pro 
    50                  55                  60                  


Asp Gly Phe Ala Pro Leu Glu Glu Thr Met Glu Cys Val Gly Cys Glu 
65                  70                  75                  80  


Val Gly His Trp Ser Glu Trp Gly Thr Cys Ser Arg Asn Asn Arg Thr 
                85                  90                  95      


Cys Gly Phe Lys Trp Gly Leu Glu Thr Arg Thr Arg Gln Ile Val Lys 
            100                 105                 110         


Lys Pro Val Lys Asp Thr Ile Leu Cys Pro Thr Ile Ala Glu Ser Arg 
        115                 120                 125             


Arg Cys Lys Met Thr Met Arg His Cys Pro Gly Gly Lys Arg Thr Pro 
    130                 135                 140                 


Lys Ala Lys Glu Lys Arg Asn Lys Lys Lys Lys Arg Lys Leu Ile Glu 
145                 150                 155                 160 


Arg Ala Gln Glu Gln His Ser Val Phe Leu Ala Thr Asp Arg Ala Asn 
                165                 170                 175     


Gln 
    


<210>  8
<211>  567
<212>  DNA
<213>  Mus musculus

<400>  8
atggacaacc tgggtgagac cttcctcagc ctggaggatg gcctggactc ttctgacacc     60

gctggtctgc tggcctcctg ggactggaaa agcagagcca ggcccttgga gctggtccag    120

gagtccccca ctcaaagcct ctccccagct ccttctctgg agtcctactc tgaggtcgca    180

ctgccctgcg ggcacagtgg ggccagcaca ggaggcagcg atggctacgg cagtcacgag    240

gctgccggct tagtcgagct ggattacagc atgttggctt ttcaacctcc ctatctacac    300

actgctggtg gcctcaaagg ccagaaaggc agcaaagtca agatgtctgt ccagcggaga    360

cggaaggcca gcgagagaga gaaactcagg atgcggacct tagccgatgc cctccacacg    420

ctccggaatt acctgccgcc tgtctacagc cagagaggcc aaccgctcac caagatccag    480

acactcaagt acaccatcaa gtacatcggg gaactcacag acctcctcaa cagcagcggg    540

agagagccca ggccacagag tgtgtga                                        567


<210>  9
<211>  582
<212>  DNA
<213>  Homo sapiens

<400>  9
atggacaacc tgcgcgagac tttcctcagc ctcgaggatg gcttgggctc ctctgacagc     60

cctggcctgc tgtcttcctg ggactggaag gacagggcag ggccctttga gctgaatcag    120

gcctccccct ctcagagcct ttccccggct ccatcgctgg aatcctattc ttcttctccc    180

tgtccagctg tggctgggct gccctgtgag cacggcgggg ccagcagtgg gggcagcgaa    240

ggctgcagtg tcggtggggc cagtggcctg gtagaggtgg actacaatat gttagctttc    300

cagcccaccc accttcaggg cggtggtggc cccaaggccc agaagggcac caaagtcagg    360

atgtctgtcc agcggaggcg gaaagccagc gagagggaga agctcaggat gaggaccttg    420

gcagatgccc tgcacaccct ccggaattac ctgccacctg tctacagcca gagaggccag    480

cctctcacca agatccagac actcaagtac accatcaagt acatcgggga actcacagac    540

ctccttaacc gcggcagaga gcccagagcc cagagcgcgt ga                       582


<210>  10
<211>  232
<212>  PRT
<213>  Mus musculus

<400>  10

Met Glu Arg Cys Pro Ser Leu Gly Val Thr Leu Tyr Ala Leu Val Val 
1               5                   10                  15      


Val Leu Gly Leu Arg Ala Ala Pro Ala Gly Gly Gln His Tyr Leu His 
            20                  25                  30          


Ile Arg Pro Ala Pro Ser Asp Asn Leu Pro Leu Val Asp Leu Ile Glu 
        35                  40                  45              


His Pro Asp Pro Ile Phe Asp Pro Lys Glu Lys Asp Leu Asn Glu Thr 
    50                  55                  60                  


Leu Leu Arg Ser Leu Leu Gly Gly His Tyr Asp Pro Gly Phe Met Ala 
65                  70                  75                  80  


Thr Ser Pro Pro Glu Asp Arg Pro Gly Gly Gly Gly Gly Pro Ala Gly 
                85                  90                  95      


Gly Ala Glu Asp Leu Ala Glu Leu Asp Gln Leu Leu Arg Gln Arg Pro 
            100                 105                 110         


Ser Gly Ala Met Pro Ser Glu Ile Lys Gly Leu Glu Phe Ser Glu Gly 
        115                 120                 125             


Leu Ala Gln Gly Lys Lys Gln Arg Leu Ser Lys Lys Leu Arg Arg Lys 
    130                 135                 140                 


Leu Gln Met Trp Leu Trp Ser Gln Thr Phe Cys Pro Val Leu Tyr Ala 
145                 150                 155                 160 


Trp Asn Asp Leu Gly Ser Arg Phe Trp Pro Arg Tyr Val Lys Val Gly 
                165                 170                 175     


Ser Cys Phe Ser Lys Arg Ser Cys Ser Val Pro Glu Gly Met Val Cys 
            180                 185                 190         


Lys Pro Ser Lys Ser Val His Leu Thr Val Leu Arg Trp Arg Cys Gln 
        195                 200                 205             


Arg Arg Gly Gly Gln Arg Cys Gly Trp Ile Pro Ile Gln Tyr Pro Ile 
    210                 215                 220                 


Ile Ser Glu Cys Lys Cys Ser Cys 
225                 230         


<210>  11
<211>  232
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Glu Arg Cys Pro Ser Leu Gly Val Thr Leu Tyr Ala Leu Val Val 
1               5                   10                  15      


Val Leu Gly Leu Arg Ala Thr Pro Ala Gly Gly Gln His Tyr Leu His 
            20                  25                  30          


Ile Arg Pro Ala Pro Ser Asp Asn Leu Pro Leu Val Asp Leu Ile Glu 
        35                  40                  45              


His Pro Asp Pro Ile Phe Asp Pro Lys Glu Lys Asp Leu Asn Glu Thr 
    50                  55                  60                  


Leu Leu Arg Ser Leu Leu Gly Gly His Tyr Asp Pro Gly Phe Met Ala 
65                  70                  75                  80  


Thr Ser Pro Pro Glu Asp Arg Pro Gly Gly Gly Gly Gly Ala Ala Gly 
                85                  90                  95      


Gly Ala Glu Asp Leu Ala Glu Leu Asp Gln Leu Leu Arg Gln Arg Pro 
            100                 105                 110         


Ser Gly Ala Met Pro Ser Glu Ile Lys Gly Leu Glu Phe Ser Glu Gly 
        115                 120                 125             


Leu Ala Gln Gly Lys Lys Gln Arg Leu Ser Lys Lys Leu Arg Arg Lys 
    130                 135                 140                 


Leu Gln Met Trp Leu Trp Ser Gln Thr Phe Cys Pro Val Leu Tyr Ala 
145                 150                 155                 160 


Trp Asn Asp Leu Gly Ser Arg Phe Trp Pro Arg Tyr Val Lys Val Gly 
                165                 170                 175     


Ser Cys Phe Ser Lys Arg Ser Cys Ser Val Pro Glu Gly Met Val Cys 
            180                 185                 190         


Lys Pro Ser Lys Ser Val His Leu Thr Val Leu Arg Trp Arg Cys Gln 
        195                 200                 205             


Arg Arg Gly Gly Gln Arg Cys Gly Trp Ile Pro Ile Gln Tyr Pro Ile 
    210                 215                 220                 


Ile Ser Glu Cys Lys Cys Ser Cys 
225                 230         


