                         SEQUENCE LISTING

<110>  Unilever PLC
       Unilever N.V.
       Hindustan Unilever Limited
 
<120>  ENZYME SYSTEM

<130>  G3142

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  3354
<212>  DNA
<213>  Pseudoalteromonas haloplanktis

<400>  1
gatcaaattc agaaaattgc atagagtgtc taaaataagg gacaattacc ctgattatac       60

gcgctatgct gcgcttggtg aatgaaaagg tgaaaaaatg tctggttttg tatttaaaca      120

atttaaagtt gaacacgacc actgtgcaat gaaagtttct accgatggca ttttgcttgg      180

cgcatgggct aatttaagcg gtgcaaagtc attacttgat ataggcaccg gcaccggctt      240

actagcacta atgtgtaagc agcgcagtag tgagcttgat ataactgcgg tagaaattga      300

cgaaagcgcc tatacccaag cattacaaaa tgtagctaat agcccgtggc caacaataag      360

tattacgcat caaactattc aaagctttag tagtgaagtt aaatttgacg tggttatctc      420

taatccaccg tattttaatc acagtttaaa aggcgataat gttgcgcgta ataccgcacg      480

ccataccgac ggcctaagtt ttgaagagtt aattaatgcg tttaaacatt taagccatag      540

aggttctcgc tttagcttaa tactcccaag cattgaaggc gcattattta ttgagcttgc      600

tactcaaaat gggctttatt taaatactca ctgccaagta aaagccacgc ccaataaaga      660

tattagccgc agtttaatga catttagcta cgtaaaaagc gatatagaaa catcaattct      720

gtgtattaga aacttagata atacctacac tgctgattat atagccctgt gcaaagcatt      780

ttatttgaaa atgtaaattg cctagttatg taggttatct agtaataaag gataatttac      840

ctattatgca tacgtattca gtttttattt aatcaattac caatctagtg ttaaccactt      900

gtaaataaca tcgagtagtt gttttgctcg ctcaacaatt ggatcacaca ctatgaaact      960

caataaaata atcaccaccg caggtttaag cctagggttg ctcttaccta gcattgcgac     1020

tgctacgccc accacatttg tacatttgtt tgaatggaat tggcaagatg tagcgcaaga     1080

atgtgagcaa tacctaggac caaaaggcta cgctgcagta caggtctcgc cacctaatga     1140

gcacattaca ggaagccaat ggtggacacg ttatcagcca gtaagttatg aactgcaaag     1200

tcgtggcgga aaccgtgcac agtttatcga tatggtaaat cgctgtagtg cagctggggt     1260

tgatatttac gttgatacgc ttattaacca tatggcagca ggaagtggca caggcacggc     1320

gggaaatagc tttggtaata aaagctttcc tatttatagc ccacaagatt ttcatgaaag     1380

ttgtaccatt aataactctg attatggcaa cgatcgctac cgagtacaaa actgtgaact     1440

agttggactt gccgatttag ataccgcttc aaactatgtg caaaatacca ttgcagcata     1500

tattaacgac ttacaagcta ttggcgtaaa aggctttcgg tttgatgctt ctaaacatgt     1560

tgcagcaagc gatatccaaa gtttaatggc caaagtaaat ggctcgccag tggtttttca     1620

agaagtgatc gatcaaggtg gggaggctgt tggtgcctct gaatatttaa gcacaggttt     1680

agtaactgaa tttaaatata gcactgagct tggtaacact tttagaaacg gctcgcttgc     1740

atggctgagt aattttggcg aagggtgggg ctttatgcca agctcttctg cggtggtttt     1800

tgtagacaat cacgacaatc agcgcgggca cggcggcgct ggcaatgtta ttacctttga     1860

agatggccgc ttatatgact tagccaatgt atttatgttg gcttatccgt acggttatcc     1920

aaaagtaatg tcgagttatg attttcatgg tgatacagat gctggtgggc caaatgtacc     1980

ggtacataat aatggtaact tagagtgttt tgcgagtaat tggaagtgtg agcatcgctg     2040

gtcatatatt gcaggcgggg tcgattttag aaataacacc gccgacaact gggcagtaac     2100

aaattggtgg gataacacaa ataatcaaat ttcatttggc cgaggtagct cgggtcatat     2160

ggctattaac aaagaagact caacacttac tgcaactgtg caaaccgata tggcgtcagg     2220

gcaatactgt aatgtgctaa aaggcgaact gtcagctgat gctaaaagtt gtagtggtga     2280

ggttataacg gttaattccg acggtactat taatcttaat attggcgctt gggatgcgat     2340

ggcaattcat aaaaatgcca agttaaatac cagttctgcc tcaagcactg aaagtgactg     2400

gcagcgaacg gttattttta ttaatgcaca aacacaaagc ggacaagata tgtttatccg     2460

tggtggaatt gaccacgctt atgcaaacgc aaaccttggt cgaaattgcc aaacaagtaa     2520

ttttgagtgt gcaatgccta ttcgtcataa caatttaaaa aatgtcacaa caagcccttg     2580

gaaagcaaat gataactacc ttgattggta tggtatagaa aatgggcaaa gtagcgaagc     2640

agaaggttct gctaccgact ggacaacgaa cgtttggcca gcaggttggg gcgctgaaaa     2700

aacggtaaac acagatggtt ttggtgtgac acctttaaat atatggggcg aacactattg     2760

gatgcttgat gtagatatgg attgcagtaa agcggttaat ggatggtttg aactaaaagc     2820

attcattaaa aatggccaag gatgggagac tgccattgca caagacaatg caccttatac     2880

aagtactaat catatggcgc aatgcggaaa aattaataaa tttgagttta ataattcagg     2940

tgtagtaatt cgtagttttt aaaagtaagc aaaaaagctc atatgaaaac ctaaactaat     3000

gattattagt ttaggttttt tgcttatgta cagcgcttta atagtgtgtg tttataaatc     3060

acctgatatg atttagccat tggccactta atttaaaagg caatacaatg aaaaagttaa     3120

ataaattagc attacttagc agcttagcag ccaccatttt tgcaagccaa ggtgtgtttg     3180

ctgctattac gcctgtaggg ctttggaaaa caattgatga ggatacaaac caagctaagt     3240

cgtatgtacg tattacccaa accagcggtg tattgagcgg caaagttgaa accattctta     3300

acccggcaaa acaaaatgat atatgtagta aatgcgatga tgagttaaaa gatc           3354


<210>  2
<211>  1555
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified AHA sequence to facilitate incorporation of the AHA gene
       into pUC19. Cloning sites (TCTAGA & GAATTC) shown at start and end.

<400>  2
tctagagtta accacttgta aataacatcg agtagttgtt ttgctcgctc aacaattgga       60

tcacacacta tgaaactcaa taaaataatc accaccgcag gtttaagcct agggttgctc      120

ttacctagca ttgcgactgc tacgcccacc acatttgtac atttgtttga atggaattgg      180

caagatgtag cgcaagaatg tgagcaatac ctaggaccaa aaggctacgc tgcagtacag      240

gtctcgccac ctaatgagca cattacagga agccaatggt ggacacgtta tcagccagta      300

agttatgaac tgcaaagtcg tggcggaaac cgtgcacagt ttatcgatat ggtaaatcgc      360

tgtagtgcag ctggggttga tatttacgtt gatacgctta ttaaccatat ggcagcagga      420

agtggcacag gcacggcggg aaatagcttt ggtaataaaa gctttcctat ttatagccca      480

caagattttc atgaaagttg taccattaat aactctgatt atggcaacga tcgctaccga      540

gtacaaaact gtgaactagt tggacttgcc gatttagata ccgcttcaaa ctatgtgcaa      600

aataccattg cagcatatat taacgactta caagctattg gcgtaaaagg ctttcggttt      660

gatgcttcta aacatgttgc agcaagcgat atccaaagtt taatggccaa agtaaatggc      720

tcgccagtgg tttttcaaga agtgatcgat caaggtgggg aggctgttgg tgcctctgaa      780

tatttaagca caggtttagt aactgaattt aaatatagca ctgagcttgg taacactttt      840

agaaacggct cgcttgcatg gctgagtaat tttggcgaag ggtggggctt tatgccaagc      900

tcttctgcgg tggtttttgt agacaatcac gacaatcagc gcgggcacgg cggcgctggc      960

aatgttatta cctttgaaga tggccgctta tatgacttag ccaatgtatt tatgttggct     1020

tatccgtacg gttatccaaa agtaatgtcg agttatgatt ttcatggtga tacagatgct     1080

ggtgggccaa atgtaccggt acataataat ggtaacttag agtgttttgc gagtaattgg     1140

aagtgtgagc atcgctggtc atatattgca ggcggggtcg attttagaaa taacaccgcc     1200

gacaactggg cagtaacaaa ttggtgggat aacacaaata atcaaatttc atttggccga     1260

ggtagctcgg gtcatatggc tattaacaaa gaagactcaa cacttactgc aactgtgcaa     1320

accgatatgg cgtcagggca atactgtaat gtgctaaaag gcgaactgtc agctgatgct     1380

aaaagttgta gtggtgaggt tataacggtt aattccgacg gtactattaa tcttaatatt     1440

ggcgcttggg atgcgatggc aattcataaa aatgccaagt taaataccag ttctgcctca     1500

agctaatagc actgaaagtg actggcagcg aacggttatt tttattaatg aattc          1555


<210>  3
<211>  4208
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of AHA, incorporated into pUC19 for expression. AHA 
       sequence shown in upper case. Xba1 and EcoR1 restriction enzyme
       sites included at start and end of AHA sequence shown

<400>  3
gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt       60

cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt      120

tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat      180

aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt      240

ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg      300

ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga      360

tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc      420

tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac      480

actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg      540

gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca      600

acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg      660

gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg      720

acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg      780

gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag      840

ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg      900

gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct      960

cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac     1020

agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact     1080

catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga     1140

tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt     1200

cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct     1260

gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc     1320

taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc     1380

ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc     1440

tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg     1500

ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt     1560

cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg     1620

agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg     1680

gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt     1740

atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag     1800

gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt     1860

gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta     1920

ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt     1980

cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc     2040

cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca     2100

acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc     2160

cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg     2220

accatgatta cgccaagctt gcatgcctgc aggtcgactc tagagttaac cacttgtaaa     2280

taacatcgag tagttgtttt gctcgctcaa caattggatc acacactatg aaactcaata     2340

aaataatcac caccgcaggt ttaagcctag ggttgctctt acctagcatt gcgactgcta     2400

cgcccaccac atttgtacat ttgtttgaat ggaattggca agatgtagcg caagaatgtg     2460

agcaatacct aggaccaaaa ggctacgctg cagtacaggt ctcgccacct aatgagcaca     2520

ttacaggaag ccaatggtgg acacgttatc agccagtaag ttatgaactg caaagtcgtg     2580

gcggaaaccg tgcacagttt atcgatatgg taaatcgctg tagtgcagct ggggttgata     2640

tttacgttga tacgcttatt aaccatatgg cagcaggaag tggcacaggc acggcgggaa     2700

atagctttgg taataaaagc tttcctattt atagcccaca agattttcat gaaagttgta     2760

ccattaataa ctctgattat ggcaacgatc gctaccgagt acaaaactgt gaactagttg     2820

gacttgccga tttagatacc gcttcaaact atgtgcaaaa taccattgca gcatatatta     2880

acgacttaca agctattggc gtaaaaggct ttcggtttga tgcttctaaa catgttgcag     2940

caagcgatat ccaaagttta atggccaaag taaatggctc gccagtggtt tttcaagaag     3000

tgatcgatca aggtggggag gctgttggtg cctctgaata tttaagcaca ggtttagtaa     3060

ctgaatttaa atatagcact gagcttggta acacttttag aaacggctcg cttgcatggc     3120

tgagtaattt tggcgaaggg tggggcttta tgccaagctc ttctgcggtg gtttttgtag     3180

acaatcacga caatcagcgc gggcacggcg gcgctggcaa tgttattacc tttgaagatg     3240

gccgcttata tgacttagcc aatgtattta tgttggctta tccgtacggt tatccaaaag     3300

taatgtcgag ttatgatttt catggtgata cagatgctgg tgggccaaat gtaccggtac     3360

ataataatgg taacttagag tgttttgcga gtaattggaa gtgtgagcat cgctggtcat     3420

atattgcagg cggggtcgat tttagaaata acaccgccga caactgggca gtaacaaatt     3480

ggtgggataa cacaaataat caaatttcat ttggccgagg tagctcgggt catatggcta     3540

ttaacaaaga agactcaaca cttactgcaa ctgtgcaaac cgatatggcg tcagggcaat     3600

actgtaatgt gctaaaaggc gaactgtcag ctgatgctaa aagttgtagt ggtgaggtta     3660

taacggttaa ttccgacggt actattaatc ttaatattgg cgcttgggat gcgatggcaa     3720

ttcataaaaa tgccaagtta aataccagtt ctgcctcaag ctaatagcac tgaaagtgac     3780

tggcagcgaa cggttatttt tattaatgaa ttcactggcc gtcgttttac aacgtcgtga     3840

ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag     3900

ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa     3960

tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg     4020

catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca     4080

cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag     4140

acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa     4200

acgcgcga                                                              4208


<210>  4
<211>  478
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Expressed amino acid sequence of the AHA derived from the 
       Pseudoalteromonas haloplanktis TAB23 gene. Secretory signal 
       peptide [MKLNKIITTAGLSLGLLLPSIATA] included at start of sequence

<400>  4

Met Lys Leu Asn Lys Ile Ile Thr Thr Ala Gly Leu Ser Leu Gly Leu 
1               5                   10                  15      


Leu Leu Pro Ser Ile Ala Thr Ala Thr Pro Thr Thr Phe Val His Leu 
            20                  25                  30          


Phe Glu Trp Asn Trp Gln Asp Val Ala Gln Glu Cys Glu Gln Tyr Leu 
        35                  40                  45              


Gly Pro Lys Gly Tyr Ala Ala Val Gln Val Ser Pro Pro Asn Glu His 
    50                  55                  60                  


Ile Thr Gly Ser Gln Trp Trp Thr Arg Tyr Gln Pro Val Ser Tyr Glu 
65                  70                  75                  80  


Leu Gln Ser Arg Gly Gly Asn Arg Ala Gln Phe Ile Asp Met Val Asn 
                85                  90                  95      


Arg Cys Ser Ala Ala Gly Val Asp Ile Tyr Val Asp Thr Leu Ile Asn 
            100                 105                 110         


His Met Ala Ala Gly Ser Gly Thr Gly Thr Ala Gly Asn Ser Phe Gly 
        115                 120                 125             


Asn Lys Ser Phe Pro Ile Tyr Ser Pro Gln Asp Phe His Glu Ser Cys 
    130                 135                 140                 


Thr Ile Asn Asn Ser Asp Tyr Gly Asn Asp Arg Tyr Arg Val Gln Asn 
145                 150                 155                 160 


Cys Glu Leu Val Gly Leu Ala Asp Leu Asp Thr Ala Ser Asn Tyr Val 
                165                 170                 175     


Gln Asn Thr Ile Ala Ala Tyr Ile Asn Asp Leu Gln Ala Ile Gly Val 
            180                 185                 190         


Lys Gly Phe Arg Phe Asp Ala Ser Lys His Val Ala Ala Ser Asp Ile 
        195                 200                 205             


Gln Ser Leu Met Ala Lys Val Asn Gly Ser Pro Val Val Phe Gln Glu 
    210                 215                 220                 


Val Ile Asp Gln Gly Gly Glu Ala Val Gly Ala Ser Glu Tyr Leu Ser 
225                 230                 235                 240 


Thr Gly Leu Val Thr Glu Phe Lys Tyr Ser Thr Glu Leu Gly Asn Thr 
                245                 250                 255     


Phe Arg Asn Gly Ser Leu Ala Trp Leu Ser Asn Phe Gly Glu Gly Trp 
            260                 265                 270         


Gly Phe Met Pro Ser Ser Ser Ala Val Val Phe Val Asp Asn His Asp 
        275                 280                 285             


Asn Gln Arg Gly His Gly Gly Ala Gly Asn Val Ile Thr Phe Glu Asp 
    290                 295                 300                 


Gly Arg Leu Tyr Asp Leu Ala Asn Val Phe Met Leu Ala Tyr Pro Tyr 
305                 310                 315                 320 


Gly Tyr Pro Lys Val Met Ser Ser Tyr Asp Phe His Gly Asp Thr Asp 
                325                 330                 335     


Ala Gly Gly Pro Asn Val Pro Val His Asn Asn Gly Asn Leu Glu Cys 
            340                 345                 350         


Phe Ala Ser Asn Trp Lys Cys Glu His Arg Trp Ser Tyr Ile Ala Gly 
        355                 360                 365             


Gly Val Asp Phe Arg Asn Asn Thr Ala Asp Asn Trp Ala Val Thr Asn 
    370                 375                 380                 


Trp Trp Asp Asn Thr Asn Asn Gln Ile Ser Phe Gly Arg Gly Ser Ser 
385                 390                 395                 400 


Gly His Met Ala Ile Asn Lys Glu Asp Ser Thr Leu Thr Ala Thr Val 
                405                 410                 415     


Gln Thr Asp Met Ala Ser Gly Gln Tyr Cys Asn Val Leu Lys Gly Glu 
            420                 425                 430         


Leu Ser Ala Asp Ala Lys Ser Cys Ser Gly Glu Val Ile Thr Val Asn 
        435                 440                 445             


Ser Asp Gly Thr Ile Asn Leu Asn Ile Gly Ala Trp Asp Ala Met Ala 
    450                 455                 460                 


Ile His Lys Asn Ala Lys Leu Asn Thr Ser Ser Ala Ser Ser 
465                 470                 475             


