                         SEQUENCE LISTING

<110>  Technische Universitt Dresden and Symbio Gruppe GmbH & Co 
       KG
       Gunzer, Florian
 
<120>  BACTERIALLY FORMED MICROCIN S, A NEW ANTIMICROBIAL PEPTIDE, 
       EFFECTIVE AGAINST PATHOGENIC MICROORGANISMS, E.G. EHEC

<130>  P31501-WO

<140>  2011-08-12
<141>  EP 11 177 451.9

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  4679
<212>  DNA
<213>  Escherichia coli

<400>  1
ttaatttttg ttcagcccat catcaatggt gcgcttgtgc ctcagctcca taaagcagcc       60

gtctgccaga tacagaaccc ggtctgcaga ggctatggtt tccgggcgat gggctatcag      120

caggacagga agccccagct gacgtagtgt ctggcttatc tgaatttcac tttcaacatc      180

aagatggctg gtcgcttcat ccagtaataa aaatcccggt ttcttgtaca aagccctggc      240

cagcagaatg cgttgttttt gccctccgga aagccctcca ccggtttcac caatcagcgt      300

ctgatacccc atcggcatag acatgatatc gctgtcgata agtgccagtc tggcgcactg      360

gatcattcgt tcatgattgc ggtctccact gaaaaaaatg atgttatctg caatggagcc      420

cctgaaaaga tggtcatcct gcagtacggt accgattcgc tgacgtacct gaaaataatc      480

cgggtgggta tgcggtatgc caaatgccct gattgttccc tcatccggga tgtggatccc      540

caggataagc ttcaccagtg tcgatttccc gcatccggat tgaccggtaa tggccatcac      600

ctcgccagga tggagtgtca gcgacacccc gcggagtatc ggtttattcc ctcccttgtg      660

gctgaaggtt atatgctcaa gggataaagg aacacgggca ccgtcggcca catccccccg      720

gaatacggac gggaagacag aagtatcatt gtcttcctga gcgggttgtt gctgatagtc      780

ttctgtcggg gtcagcacaa tgtcagccag gcgttcgttg tagatatcaa gcatacgcca      840

ggaaaaatac ttatcggtca ggctgctgat actggatgaa aaacgcatct ggtaggacaa      900

ataagcaacc agcatgccga cggtgaaggt accgttcagt acttccccgg cacctttcca      960

caaaataatc gctgatacca cgctgccggt cagggtgtgt gcaatgtcat agcacatcag     1020

ctgacggctc tgtcgcagct gcgtattcct gcgaacaatg ttgaggttca gccaggcggc     1080

ctcacggtgc gctgtcacgc cgttaattct caggctcaga ataccgttga gggtttcaag     1140

aaaatgcccg gactccctgg cacctgcgtc ccaggcatct tctgccgact gtcgaaggga     1200

cggataccac aacgccctca gcacaccgta gataatggct gccagtacgg ctatcagcgt     1260

catttccgga ctgtacagca gcatcataca gagcgcagtt atcacgagca acacgtccag     1320

aataccttcc agtacctgcg tggtcagcgc ctcctggatg gtatccacgg cctcaaaacg     1380

ggcattgata cttcccttac tgcgcgcatc aaaccagccc agcgggagac gcaccagatg     1440

atggaaaacc cgggctgtcc actgcatgtt aaaattgacg gacagagtta tactggccca     1500

ctggcgtgcc agcgacagca gcatctgggt cattgacagc agcagcaggg caatgattat     1560

aacggtcagg agacttcgat cggcggcaac cagcacttca tcaattacca gctggttgag     1620

aagtggacta ctcagcgtca ggatttccag cgccagggca aaaatgatga tgcgcgacat     1680

cgcggccagc agcccgggtg tcctgcctgt cagttggcgc aggcgaattt tttttctttc     1740

atcccgggcg gtaaaatcac tggccggcgt caactccagc gcgacaccgg tgaaatgttt     1800

tcctgcatcc tgcagggaga gcgttatctt ccctttgtcc gggtcgtgga taaccagcct     1860

gctcccgcga actttataaa gcacgacgaa atggttcatg ttccagtgga gaatgcacgg     1920

gagagaaagg gatttcaggt cttccggctc cagccggacg gcccgggagg acaaccggat     1980

accggctgca cactctatca gcctctgcag ggtcatcccc tgtgtgctga tactgaaacg     2040

ttcgcgcagg gtgggtaaat ctgtctgcag accatgccag caggccatca tgaccagaca     2100

tgccagacca cattccgcag actctgtctg ccggatgagg gggagctgtt ttctcccggt     2160

ccatttaagg gtatccatta cagttttcct ttcaggctcc agagtggctc ggtcagccac     2220

tcccacaaat ggcgggtatc caggctgaca tccccctcaa gggtcatgcc ggggcgcagg     2280

ggttcctgtt ttccataagc cagaatgaag gtattctcag gttcgacgat gacgcggtag     2340

tgcccttcat tctctttcca ggtcacaggg gatacggaga gtaaatccga aggggtcagc     2400

gttgtacggc ttatctctcg gatagtcccg tactgaacgc caaacttctg ataggggaat     2460

gcggcgaacc gaagtgcaac acgctgcccc ggctgaataa aaccggcatt ctgactggtg     2520

gcatagagtt ctatctgcag acgagcattg tcgggaacca gagtcatgac cggttcagat     2580

gccctgacag actgtccctg tctgatcagc acggcagcaa cggttccgga taccggtgac     2640

gtcagcgtaa agttctcctg tccggccagt tcgtcctgct gttgcctgat cccctgcaac     2700

tgcctgtcca gctcagcttt acggctttcc ccctgaacga tgagatggtt aagatcttct     2760

tcagcagcct ccattgcagt gtgtagttgc agaagcccct gacgctgatc ctcaacgttt     2820

tgcagtgacg tagaaacgtc aatttgcttc tgctggtatt cgatatctga tacgtaatgc     2880

gttccagcca gttttttata gcgctccatg acggacacgg ccagcgtggc ctgatgcccg     2940

gcaagcgaca ggcgcagctc tgcactttta atctgtggtt tcagggaggc aattcgctgt     3000

agtatggcct gctgctgctg actgttgtca cgcaattcca gagtctgctg ggaagacagc     3060

atggcatact gagtcttcag ggacatgctc atggctgcca gtgtacctgt gccctgcccg     3120

ttgtaatgct cgccgctgat atggtaaaca ggctcaccgg cagctacgtg ctgaccttct     3180

gaaacagtct gtcgggtgac gtatccggca tattgcggtg tgattttcac cagtcctgat     3240

gaaggcatca ctatacctgt aagatgagcc ttgcgggtat aactaccata ataaacgaat     3300

aatgagacgc tcagaaatat aagtaatgta gcaacggcgc atactgacat gccaaatgac     3360

gcgggtaaga caatatctcc gtactctgta tcactgttat gttctaatgc ttcctccctg     3420

aatatactca tggttataaa atattttgaa tatttaataa tataacaaag gtattcatgc     3480

ctgaatgtat taacatagga taaaaaaggg atctgctttt aattctgacc cctaataaca     3540

gcatagatac aacaaaaagg actgattgat ccagaacatt ataatactga aagtgcatca     3600

gagagaaaaa caaagatgtt atcacacagg gaatgataat gttttccttg aaaattgaac     3660

gcagaaatcc aaaggcacag gctctgtata cgatctcctc atagtagggt acgaccagtg     3720

tcatcactaa aactgttatc cagtttatct gtgatggttc gtaatgatat aaatagtctc     3780

tgtagcagta aacagctaac tgtatcagaa taattataag gaatgacagg gctgaaaatt     3840

taactgtagc tgagtctgtt cttattttta ttctacactc aggaaattcc tttaggtaaa     3900

ataagtaaac aagagttgaa attagaatct ctgtaatgaa cacaatgaaa aatgcaatat     3960

ccagtccaac ataacgcagt gtaaaggttg gcgataatgt tacaatagtt gatataataa     4020

ccactgcaag aaaaataata gcgctgtatt tactatatct aaactggctc gaacgttcat     4080

ccattacaaa acatcacttt atgaaggtta ttatttttgc taaaactata tctgtaaatt     4140

tagcagtgga aaacaaggtg atcaatgtaa ttactatttt tgaaaattcc tcaggcaacg     4200

aaaaccatga caaatcaata atggaggaac cacttaatag cgcaaaaccc agtaataaac     4260

ttaatataaa agacaataat ttaataaaca ctgcagtatc ctccgttgat acttacttat     4320

cgactacagg taccaccgac gttacttcct gaacaattac tggaaccagc tctgtttccg     4380

cctccgttac catttcctcc accggagaga cactggccga taactgcccc accaatagtt     4440

cctctggtca ttcctacagg accaccttta atggcaccac caaccattcc actaaagaca     4500

gcattagcac atttaactgt gctcgggtca ctgtaaatgt gtgtcggtgc atttcgtccc     4560

agtgagttac gggctccggt gttacgacta cgacttccgc ctccttcata attactgttt     4620

gcgttgccac cactgactaa tgcaatttca tcaaaactca attctctgat atttgacat      4679


<210>  2
<211>  363
<212>  DNA
<213>  Escherichia coli

<400>  2
atgtcaaata tcagagaatt gagttttgat gaaattgcat tagtcagtgg tggcaacgca       60

aacagtaatt atgaaggagg cggaagtcgt agtcgtaaca ccggagcccg taactcactg      120

ggacgaaatg caccgacaca catttacagt gacccgagca cagttaaatg tgctaatgct      180

gtctttagtg gaatggttgg tggtgccatt aaaggtggtc ctgtaggaat gaccagagga      240

actattggtg gggcagttat cggccagtgt ctctccggtg gaggaaatgg taacggaggc      300

ggaaacagag ctggttccag taattgttca ggaagtaacg tcggtggtac ctgtagtcga      360

taa                                                                    363


<210>  3
<211>  651
<212>  DNA
<213>  Escherichia coli

<400>  3
atggatgaac gttcgagcca gtttagatat agtaaataca gcgctattat ttttcttgca       60

gtggttatta tatcaactat tgtaacatta tcgccaacct ttacactgcg ttatgttgga      120

ctggatattg catttttcat tgtgttcatt acagagattc taatttcaac tcttgtttac      180

ttattttacc taaaggaatt tcctgagtgt agaataaaaa taagaacaga ctcagctaca      240

gttaaatttt cagccctgtc attccttata attattctga tacagttagc tgtttactgc      300

tacagagact atttatatca ttacgaacca tcacagataa actggataac agttttagtg      360

atgacactgg tcgtacccta ctatgaggag atcgtataca gagcctgtgc ctttggattt      420

ctgcgttcaa ttttcaagga aaacattatc attccctgtg tgataacatc tttgtttttc      480

tctctgatgc actttcagta ttataatgtt ctggatcaat cagtcctttt tgttgtatct      540

atgctgttat taggggtcag aattaaaagc agatcccttt tttatcctat gttaatacat      600

tcaggcatga atacctttgt tatattatta aatattcaaa atattttata a               651


<210>  4
<211>  1254
<212>  DNA
<213>  Escherichia coli

<400>  4
atgagtatat tcagggagga agcattagaa cataacagtg atacagagta cggagatatt       60

gtcttacccg cgtcatttgg catgtcagta tgcgccgttg ctacattact tatatttctg      120

agcgtctcat tattcgttta ttatggtagt tatacccgca aggctcatct tacaggtata      180

gtgatgcctt catcaggact ggtgaaaatc acaccgcaat atgccggata cgtcacccga      240

cagactgttt cagaaggtca gcacgtagct gccggtgagc ctgtttacca tatcagcggc      300

gagcattaca acgggcaggg cacaggtaca ctggcagcca tgagcatgtc cctgaagact      360

cagtatgcca tgctgtcttc ccagcagact ctggaattgc gtgacaacag tcagcagcag      420

caggccatac tacagcgaat tgcctccctg aaaccacaga ttaaaagtgc agagctgcgc      480

ctgtcgcttg ccgggcatca ggccacgctg gccgtgtccg tcatggagcg ctataaaaaa      540

ctggctggaa cgcattacgt atcagatatc gaataccagc agaagcaaat tgacgtttct      600

acgtcactgc aaaacgttga ggatcagcgt caggggcttc tgcaactaca cactgcaatg      660

gaggctgctg aagaagatct taaccatctc atcgttcagg gggaaagccg taaagctgag      720

ctggacaggc agttgcaggg gatcaggcaa cagcaggacg aactggccgg acaggagaac      780

tttacgctga cgtcaccggt atccggaacc gttgctgccg tgctgatcag acagggacag      840

tctgtcaggg catctgaacc ggtcatgact ctggttcccg acaatgctcg tctgcagata      900

gaactctatg ccaccagtca gaatgccggt tttattcagc cggggcagcg tgttgcactt      960

cggttcgccg cattccccta tcagaagttt ggcgttcagt acgggactat ccgagagata     1020

agccgtacaa cgctgacccc ttcggattta ctctccgtat cccctgtgac ctggaaagag     1080

aatgaagggc actaccgcgt catcgtcgaa cctgagaata ccttcattct ggcttatgga     1140

aaacaggaac ccctgcgccc cggcatgacc cttgaggggg atgtcagcct ggatacccgc     1200

catttgtggg agtggctgac cgagccactc tggagcctga aaggaaaact gtaa           1254


<210>  5
<211>  2178
<212>  DNA
<213>  Escherichia coli

<400>  5
atggataccc ttaaatggac cgggagaaaa cagctccccc tcatccggca gacagagtct       60

gcggaatgtg gtctggcatg tctggtcatg atggcctgct ggcatggtct gcagacagat      120

ttacccaccc tgcgcgaacg tttcagtatc agcacacagg ggatgaccct gcagaggctg      180

atagagtgtg cagccggtat ccggttgtcc tcccgggccg tccggctgga gccggaagac      240

ctgaaatccc tttctctccc gtgcattctc cactggaaca tgaaccattt cgtcgtgctt      300

tataaagttc gcgggagcag gctggttatc cacgacccgg acaaagggaa gataacgctc      360

tccctgcagg atgcaggaaa acatttcacc ggtgtcgcgc tggagttgac gccggccagt      420

gattttaccg cccgggatga aagaaaaaaa attcgcctgc gccaactgac aggcaggaca      480

cccgggctgc tggccgcgat gtcgcgcatc atcatttttg ccctggcgct ggaaatcctg      540

acgctgagta gtccacttct caaccagctg gtaattgatg aagtgctggt tgccgccgat      600

cgaagtctcc tgaccgttat aatcattgcc ctgctgctgc tgtcaatgac ccagatgctg      660

ctgtcgctgg cacgccagtg ggccagtata actctgtccg tcaattttaa catgcagtgg      720

acagcccggg ttttccatca tctggtgcgt ctcccgctgg gctggtttga tgcgcgcagt      780

aagggaagta tcaatgcccg ttttgaggcc gtggatacca tccaggaggc gctgaccacg      840

caggtactgg aaggtattct ggacgtgttg ctcgtgataa ctgcgctctg tatgatgctg      900

ctgtacagtc cggaaatgac gctgatagcc gtactggcag ccattatcta cggtgtgctg      960

agggcgttgt ggtatccgtc ccttcgacag tcggcagaag atgcctggga cgcaggtgcc     1020

agggagtccg ggcattttct tgaaaccctc aacggtattc tgagcctgag aattaacggc     1080

gtgacagcgc accgtgaggc cgcctggctg aacctcaaca ttgttcgcag gaatacgcag     1140

ctgcgacaga gccgtcagct gatgtgctat gacattgcac acaccctgac cggcagcgtg     1200

gtatcagcga ttattttgtg gaaaggtgcc ggggaagtac tgaacggtac cttcaccgtc     1260

ggcatgctgg ttgcttattt gtcctaccag atgcgttttt catccagtat cagcagcctg     1320

accgataagt atttttcctg gcgtatgctt gatatctaca acgaacgcct ggctgacatt     1380

gtgctgaccc cgacagaaga ctatcagcaa caacccgctc aggaagacaa tgatacttct     1440

gtcttcccgt ccgtattccg gggggatgtg gccgacggtg cccgtgttcc tttatccctt     1500

gagcatataa ccttcagcca caagggaggg aataaaccga tactccgcgg ggtgtcgctg     1560

acactccatc ctggcgaggt gatggccatt accggtcaat ccggatgcgg gaaatcgaca     1620

ctggtgaagc ttatcctggg gatccacatc ccggatgagg gaacaatcag ggcatttggc     1680

ataccgcata cccacccgga ttattttcag gtacgtcagc gaatcggtac cgtactgcag     1740

gatgaccatc ttttcagggg ctccattgca gataacatca tttttttcag tggagaccgc     1800

aatcatgaac gaatgatcca gtgcgccaga ctggcactta tcgacagcga tatcatgtct     1860

atgccgatgg ggtatcagac gctgattggt gaaaccggtg gagggctttc cggagggcaa     1920

aaacaacgca ttctgctggc cagggctttg tacaagaaac cgggattttt attactggat     1980

gaagcgacca gccatcttga tgttgaaagt gaaattcaga taagccagac actacgtcag     2040

ctggggcttc ctgtcctgct gatagcccat cgcccggaaa ccatagcctc tgcagaccgg     2100

gttctgtatc tggcagacgg ctgctttatg gagctgaggc acaagcgcac cattgatgat     2160

gggctgaaca aaaattaa                                                   2178


<210>  6
<211>  120
<212>  PRT
<213>  Escherichia coli

<400>  6

Met Ser Asn Ile Arg Glu Leu Ser Phe Asp Glu Ile Ala Leu Val Ser 
1               5                   10                  15      


Gly Gly Asn Ala Asn Ser Asn Tyr Glu Gly Gly Gly Ser Arg Ser Arg 
            20                  25                  30          


Asn Thr Gly Ala Arg Asn Ser Leu Gly Arg Asn Ala Pro Thr His Ile 
        35                  40                  45              


Tyr Ser Asp Pro Ser Thr Val Lys Cys Ala Asn Ala Val Phe Ser Gly 
    50                  55                  60                  


Met Val Gly Gly Ala Ile Lys Gly Gly Pro Val Gly Met Thr Arg Gly 
65                  70                  75                  80  


Thr Ile Gly Gly Ala Val Ile Gly Gln Cys Leu Ser Gly Gly Gly Asn 
                85                  90                  95      


Gly Asn Gly Gly Gly Asn Arg Ala Gly Ser Ser Asn Cys Ser Gly Ser 
            100                 105                 110         


Asn Val Gly Gly Thr Cys Ser Arg 
        115                 120 


<210>  7
<211>  102
<212>  PRT
<213>  Escherichia coli

<400>  7

Asn Ala Asn Ser Asn Tyr Glu Gly Gly Gly Ser Arg Ser Arg Asn Thr 
1               5                   10                  15      


Gly Ala Arg Asn Ser Leu Gly Arg Asn Ala Pro Thr His Ile Tyr Ser 
            20                  25                  30          


Asp Pro Ser Thr Val Lys Cys Ala Asn Ala Val Phe Ser Gly Met Val 
        35                  40                  45              


Gly Gly Ala Ile Lys Gly Gly Pro Val Gly Met Thr Arg Gly Thr Ile 
    50                  55                  60                  


Gly Gly Ala Val Ile Gly Gln Cys Leu Ser Gly Gly Gly Asn Gly Asn 
65                  70                  75                  80  


Gly Gly Gly Asn Arg Ala Gly Ser Ser Asn Cys Ser Gly Ser Asn Val 
                85                  90                  95      


Gly Gly Thr Cys Ser Arg 
            100         


<210>  8
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSYM1 sequencing primer

<400>  8
cagctggata tcctgcgcg                                                    19


<210>  9
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSYM1 sequencing primer

<400>  9
ggttgcccgg catccaacg                                                    19


<210>  10
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAZ6/pAZ8 cloning primer

<400>  10
tcaattgtgt cgactcaatt actcttgtga g                                      31


<210>  11
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAZ6 cloning primer

<400>  11
catgtaatag tgctagcatg ttaaaattta taag                                   34


<210>  12
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAZ8, pAZ12 cloning primer

<400>  12
caaaaataat agctagcaag tgatgttttg taatg                                  35


<210>  13
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAZ9 cloning primer

<400>  13
ctcgaattca tccattacaa aacatcac                                          28


<210>  14
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAZ9 cloning primer

<400>  14
ctggctgcag taattgttca ggaagtaacg                                        30


<210>  15
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAZ10 cloning primer

<400>  15
taggaattca gaggaactat tggtggg                                           27


<210>  16
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAZ10 cloning primer

<400>  16
ctccgctgca gacttactta tcgactacag gtaccac                                37


<210>  17
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAZ11 cloning primer

<400>  17
gttagaattc ataagaggga tttttatgtc aaatatc                                37


<210>  18
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAZ11 cloning primer

<400>  18
gttgatactg cagcttatcg actacaggta ccacc                                  35


<210>  19
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAZ12 cloning primer

<400>  19
cccaagctta gttaaatgtg ctaatgctgt c                                      31


<210>  20
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAZ12 cloning primer

<400>  20
ggcatcggtc gacgcaac                                                     18


<210>  21
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAZ14 cloning primer

<400>  21
cattgctagc catcacagat aaactggata ac                                     32


<210>  22
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAZ13, pAZ14 cloning primer

<400>  22
ccctgagtcg actcatggtt ataaaatatt ttg                                    33


<210>  23
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  inhibition control

<400>  23
atggctatcg acgaaaacaa ac                                                22


<210>  24
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  inhibition control

<400>  24
ttaaaaatct tcgttagttt ctgc                                              24


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mcsS screening primer

<400>  25
atgtcaaata tcagagaatt gag                                               23


<210>  26
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mcsS screening primer

<400>  26
ttatcgacta caggtaccac c                                                 21


