                         SEQUENCE LISTING

<110>  Life Technologies Corporation
       VANDER HORN, Peter
       NIKIFOROV, Theo
       LUO, Guobin
       LANDES, Mindy
       MAZUR, Daniel
       TOZER, Eileen
       LINCECUM, Tommie
 
<120>  POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME

<130>  LT00556PCT

<150>  61/522,125
<151>  2011-08-10

<150>  61/545,434
<151>  2011-10-10

<150>  61/585,133
<151>  2012-01-10

<150>  61/643,844
<151>  2012-05-07

<150>  61/681,593
<151>  2012-08-09

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  581
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  1

Met Ala Lys Met Ala Phe Thr Leu Ala Asp Arg Val Thr Glu Glu Met 
1               5                   10                  15      


Leu Ala Asp Lys Ala Ala Leu Val Val Glu Val Val Glu Glu Asn Tyr 
            20                  25                  30          


His Asp Ala Pro Ile Val Gly Ile Ala Val Val Asn Glu His Gly Arg 
        35                  40                  45              


Phe Phe Leu Arg Pro Glu Thr Ala Leu Ala Asp Pro Gln Phe Val Ala 
    50                  55                  60                  


Trp Leu Gly Asp Glu Thr Lys Lys Lys Ser Met Phe Asp Ser Lys Arg 
65                  70                  75                  80  


Ala Ala Val Ala Leu Lys Trp Lys Gly Ile Glu Leu Cys Gly Val Ser 
                85                  90                  95      


Phe Asp Leu Leu Leu Ala Ala Tyr Leu Leu Asp Pro Ala Gln Gly Val 
            100                 105                 110         


Asp Asp Val Ala Ala Ala Ala Lys Met Lys Gln Tyr Glu Ala Val Arg 
        115                 120                 125             


Pro Asp Glu Ala Val Tyr Gly Lys Gly Ala Lys Arg Ala Val Pro Asp 
    130                 135                 140                 


Glu Pro Val Leu Ala Glu His Leu Val Arg Lys Ala Ala Ala Ile Trp 
145                 150                 155                 160 


Glu Leu Glu Arg Pro Phe Leu Asp Glu Leu Arg Arg Asn Glu Gln Asp 
                165                 170                 175     


Arg Leu Leu Val Glu Leu Glu Gln Pro Leu Ser Ser Ile Leu Ala Glu 
            180                 185                 190         


Met Glu Phe Ala Gly Val Lys Val Asp Thr Lys Arg Leu Glu Gln Met 
        195                 200                 205             


Gly Lys Glu Leu Ala Glu Gln Leu Gly Thr Val Glu Gln Arg Ile Tyr 
    210                 215                 220                 


Glu Leu Ala Gly Gln Glu Phe Asn Ile Asn Ser Pro Lys Gln Leu Gly 
225                 230                 235                 240 


Val Ile Leu Phe Glu Lys Leu Gln Leu Pro Val Leu Lys Lys Thr Lys 
                245                 250                 255     


Thr Gly Tyr Ser Thr Ser Ala Asp Val Leu Glu Lys Leu Ala Pro Tyr 
            260                 265                 270         


His Glu Ile Val Glu Asn Ile Leu His Tyr Arg Gln Leu Gly Lys Leu 
        275                 280                 285             


Gln Ser Thr Tyr Ile Glu Gly Leu Leu Lys Val Val Arg Pro Asp Thr 
    290                 295                 300                 


Lys Lys Val His Thr Ile Phe Asn Gln Ala Leu Thr Gln Thr Gly Arg 
305                 310                 315                 320 


Leu Ser Ser Thr Glu Pro Asn Leu Gln Asn Ile Pro Ile Arg Leu Glu 
                325                 330                 335     


Glu Gly Arg Lys Ile Arg Gln Ala Phe Val Pro Ser Glu Ser Asp Trp 
            340                 345                 350         


Leu Ile Phe Ala Ala Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala 
        355                 360                 365             


His Ile Ala Glu Asp Asp Asn Leu Met Glu Ala Phe Arg Arg Asp Leu 
    370                 375                 380                 


Asp Ile His Thr Lys Thr Ala Met Asp Ile Phe Gln Val Ser Glu Asp 
385                 390                 395                 400 


Glu Val Thr Pro Asn Met Arg Arg Gln Ala Lys Ala Val Asn Phe Gly 
                405                 410                 415     


Ile Val Tyr Gly Ile Ser Asp Tyr Gly Leu Ala Gln Asn Leu Asn Ile 
            420                 425                 430         


Ser Arg Lys Glu Ala Ala Glu Phe Ile Glu Arg Tyr Phe Glu Ser Phe 
        435                 440                 445             


Pro Gly Val Lys Arg Tyr Met Glu Asn Ile Val Gln Glu Ala Lys Gln 
    450                 455                 460                 


Lys Gly Tyr Val Thr Thr Leu Leu His Arg Arg Arg Tyr Leu Pro Asp 
465                 470                 475                 480 


Ile Thr Ser Arg Asn Phe Asn Val Arg Ser Phe Ala Glu Arg Met Ala 
                485                 490                 495     


Met Asn Thr Pro Ile Gln Gly Ser Ala Ala Asp Ile Ile Lys Lys Ala 
            500                 505                 510         


Met Ile Asp Leu Asn Ala Arg Leu Lys Glu Glu Arg Leu Gln Ala His 
        515                 520                 525             


Leu Leu Leu Gln Val His Asp Glu Leu Ile Leu Glu Ala Pro Lys Glu 
    530                 535                 540                 


Glu Met Glu Arg Leu Cys Arg Leu Val Pro Glu Val Met Glu Gln Ala 
545                 550                 555                 560 


Val Thr Leu Arg Val Pro Leu Lys Val Asp Tyr His Tyr Gly Ser Thr 
                565                 570                 575     


Trp Tyr Asp Ala Lys 
            580     


<210>  2
<211>  581
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  2

Met Ala Lys Met Ala Phe Thr Leu Ala Asp Arg Val Thr Glu Glu Met 
1               5                   10                  15      


Leu Ala Asp Lys Ala Ala Leu Val Val Glu Val Val Glu Glu Asn Tyr 
            20                  25                  30          


His Asp Ala Pro Ile Val Gly Ile Ala Val Val Asn Glu Arg Gly Arg 
        35                  40                  45              


Phe Phe Leu Arg Pro Glu Thr Ala Leu Ala Asp Pro Gln Phe Val Ala 
    50                  55                  60                  


Trp Leu Gly Asp Glu Thr Lys Lys Lys Ser Met Phe Asp Ser Lys Arg 
65                  70                  75                  80  


Ala Ala Val Ala Leu Lys Trp Lys Gly Ile Glu Leu Cys Gly Val Ser 
                85                  90                  95      


Phe Asp Leu Leu Leu Ala Ala Tyr Leu Leu Asp Pro Ala Gln Gly Val 
            100                 105                 110         


Asp Asp Val Ala Ala Ala Ala Lys Met Lys Gln Tyr Glu Ala Val Arg 
        115                 120                 125             


Pro Asp Glu Ala Val Tyr Gly Lys Gly Ala Lys Arg Ala Val Pro Asp 
    130                 135                 140                 


Glu Pro Val Leu Ala Glu His Leu Val Arg Lys Ala Ala Ala Ile Trp 
145                 150                 155                 160 


Glu Leu Glu Arg Pro Phe Leu Asp Glu Leu Arg Arg Asn Glu Gln Asp 
                165                 170                 175     


Arg Leu Leu Val Glu Leu Glu Gln Pro Leu Ser Ser Ile Leu Ala Glu 
            180                 185                 190         


Met Glu Phe Ala Gly Val Lys Val Asp Thr Lys Arg Leu Glu Gln Met 
        195                 200                 205             


Gly Lys Glu Leu Ala Glu Gln Leu Gly Thr Val Glu Gln Arg Ile Tyr 
    210                 215                 220                 


Glu Leu Ala Gly Gln Glu Phe Asn Ile Asn Ser Pro Lys Gln Leu Gly 
225                 230                 235                 240 


Val Ile Leu Phe Glu Lys Leu Gln Leu Pro Val Leu Lys Lys Thr Lys 
                245                 250                 255     


Thr Gly Tyr Ser Thr Ser Ala Asp Val Leu Glu Lys Leu Ala Pro Tyr 
            260                 265                 270         


His Glu Ile Val Glu Asn Ile Leu His Tyr Arg Gln Leu Gly Lys Leu 
        275                 280                 285             


Gln Ser Thr Tyr Ile Glu Gly Leu Leu Lys Val Val Arg Pro Asp Thr 
    290                 295                 300                 


Lys Lys Val His Thr Ile Phe Asn Gln Ala Leu Thr Gln Thr Gly Arg 
305                 310                 315                 320 


Leu Ser Ser Thr Glu Pro Asn Leu Gln Asn Ile Pro Ile Arg Leu Glu 
                325                 330                 335     


Glu Gly Arg Lys Ile Arg Gln Ala Phe Val Pro Ser Glu Ser Asp Trp 
            340                 345                 350         


Leu Ile Phe Ala Ala Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala 
        355                 360                 365             


His Ile Ala Glu Asp Asp Asn Leu Met Glu Ala Phe Arg Arg Asp Leu 
    370                 375                 380                 


Asp Ile His Thr Lys Thr Ala Met Asp Ile Phe Gln Val Ser Glu Asp 
385                 390                 395                 400 


Glu Val Thr Pro Asn Met Arg Arg Gln Ala Lys Ala Val Asn Phe Gly 
                405                 410                 415     


Ile Val Tyr Gly Ile Ser Asp Tyr Gly Leu Ala Gln Asn Leu Asn Ile 
            420                 425                 430         


Ser Arg Lys Glu Ala Ala Glu Phe Ile Glu Arg Tyr Phe Gln Ser Phe 
        435                 440                 445             


Pro Gly Val Lys Arg Tyr Met Glu Asn Ile Val Gln Glu Ala Lys Gln 
    450                 455                 460                 


Lys Gly Tyr Val Thr Thr Leu Leu His Arg Arg Arg Tyr Leu Pro Asp 
465                 470                 475                 480 


Ile Thr Ser Arg Asn Phe Asn Val Arg Ser Phe Ala Glu Arg Met Ala 
                485                 490                 495     


Met Asn Thr Pro Ile Gln Gly Ser Ala Ala Asp Ile Ile Lys Lys Ala 
            500                 505                 510         


Met Ile Asp Leu Asn Ala Arg Leu Lys Glu Glu Arg Leu Gln Ala His 
        515                 520                 525             


Leu Leu Leu Gln Val His Asp Glu Leu Ile Leu Glu Ala Pro Lys Glu 
    530                 535                 540                 


Glu Met Glu Arg Leu Cys Arg Leu Val Pro Glu Val Met Glu Gln Ala 
545                 550                 555                 560 


Val Thr Leu Arg Val Pro Leu Lys Val Asp Tyr Arg Tyr Gly Ser Thr 
                565                 570                 575     


Trp Tyr Asp Ala Lys 
            580     


<210>  3
<211>  581
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  3

Met Ala Lys Met Ala Phe Thr Leu Ala Asp Arg Val Thr Glu Glu Met 
1               5                   10                  15      


Leu Ala Asp Lys Ala Ala Leu Val Val Glu Val Val Glu Glu Asn Tyr 
            20                  25                  30          


His Asp Ala Pro Ile Val Gly Ile Ala Val Val Asn Glu Arg Gly Arg 
        35                  40                  45              


Phe Phe Leu Arg Pro Glu Thr Ala Leu Ala Asp Pro Gln Phe Val Ala 
    50                  55                  60                  


Trp Leu Gly Asp Glu Thr Lys Lys Lys Ser Met Phe Asp Ser Lys Arg 
65                  70                  75                  80  


Ala Ala Val Ala Leu Lys Trp Lys Gly Ile Glu Leu Cys Gly Val Ser 
                85                  90                  95      


Phe Asp Leu Leu Leu Ala Ala Tyr Leu Leu Asp Pro Ala Gln Gly Val 
            100                 105                 110         


Asp Asp Val Ala Ala Ala Ala Lys Met Lys Gln Tyr Glu Ala Val Arg 
        115                 120                 125             


Pro Asp Glu Ala Val Tyr Gly Lys Gly Ala Lys Arg Ala Val Pro Asp 
    130                 135                 140                 


Glu Pro Val Leu Ala Glu His Leu Val Arg Lys Ala Ala Ala Ile Trp 
145                 150                 155                 160 


Glu Leu Glu Arg Pro Phe Leu Asp Glu Leu Arg Arg Asn Glu Gln Asp 
                165                 170                 175     


Arg Leu Leu Val Glu Leu Glu Gln Pro Leu Ser Ser Ile Leu Ala Glu 
            180                 185                 190         


Met Glu Phe Ala Gly Val Lys Val Asp Thr Lys Arg Leu Glu Gln Met 
        195                 200                 205             


Gly Lys Glu Leu Ala Glu Gln Leu Gly Thr Val Glu Gln Arg Ile Tyr 
    210                 215                 220                 


Glu Leu Ala Gly Gln Glu Phe Asn Ile Asn Ser Pro Lys Gln Leu Gly 
225                 230                 235                 240 


Val Ile Leu Phe Glu Lys Leu Gln Leu Pro Val Leu Lys Lys Thr Lys 
                245                 250                 255     


Thr Gly Tyr Ser Thr Ser Ala Asp Val Leu Glu Lys Leu Ala Pro Tyr 
            260                 265                 270         


His Glu Ile Val Glu Asn Ile Leu His Tyr Arg Gln Leu Gly Lys Leu 
        275                 280                 285             


Gln Ser Thr Tyr Ile Glu Gly Leu Leu Lys Val Val Arg Pro Asp Thr 
    290                 295                 300                 


Lys Lys Val His Thr Ile Phe Asn Gln Ala Leu Thr Gln Thr Gly Arg 
305                 310                 315                 320 


Leu Ser Ser Thr Glu Pro Asn Leu Gln Asn Ile Pro Ile Arg Leu Glu 
                325                 330                 335     


Glu Gly Arg Lys Ile Arg Gln Ala Phe Val Pro Ser Glu Ser Asp Trp 
            340                 345                 350         


Leu Ile Phe Ala Ala Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala 
        355                 360                 365             


His Ile Ala Glu Asp Asp Asn Leu Met Glu Ala Phe Arg Arg Asp Leu 
    370                 375                 380                 


Asp Ile His Thr Lys Thr Ala Met Asp Ile Phe Gln Val Ser Glu Asp 
385                 390                 395                 400 


Glu Val Thr Pro Asn Met Arg Arg Gln Ala Lys Ala Val Asn Phe Gly 
                405                 410                 415     


Ile Val Tyr Gly Ile Ser Asp Tyr Gly Leu Ala Gln Asn Leu Asn Ile 
            420                 425                 430         


Ser Arg Lys Glu Ala Ala Glu Phe Ile Glu Arg Tyr Phe Gln Ser Phe 
        435                 440                 445             


Pro Gly Val Lys Arg Tyr Met Glu Asn Ile Val Gln Glu Ala Lys Gln 
    450                 455                 460                 


Lys Gly Tyr Val Thr Thr Leu Leu Arg Arg Arg Arg Tyr Leu Pro Asp 
465                 470                 475                 480 


Ile Thr Ser Arg Asn Phe Asn Val Arg Ser Phe Ala Glu Arg Met Ala 
                485                 490                 495     


Met Asn Thr Pro Ile Gln Gly Ser Ala Ala Asp Ile Ile Lys Lys Ala 
            500                 505                 510         


Met Ile Asp Leu Asn Ala Arg Leu Lys Glu Glu Arg Leu Gln Ala Ala 
        515                 520                 525             


Leu Leu Leu Gln Val His Asp Glu Leu Ile Leu Glu Ala Pro Lys Glu 
    530                 535                 540                 


Glu Met Glu Arg Leu Cys Arg Leu Val Pro Glu Val Met Glu Gln Ala 
545                 550                 555                 560 


Val Thr Leu Arg Val Pro Leu Lys Val Asp Tyr Arg Tyr Gly Ser Thr 
                565                 570                 575     


Trp Tyr Asp Ala Lys 
            580     


<210>  4
<211>  581
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  4

Met Ala Lys Met Ala Phe Thr Leu Ala Asp Arg Val Thr Glu Glu Met 
1               5                   10                  15      


Leu Ala Asp Lys Ala Ala Leu Val Val Glu Val Val Glu Glu Asn Tyr 
            20                  25                  30          


His Asp Ala Pro Ile Val Gly Ile Ala Val Val Asn Glu Arg Gly Arg 
        35                  40                  45              


Phe Phe Leu Arg Pro Glu Thr Ala Leu Ala Asp Pro Gln Phe Val Ala 
    50                  55                  60                  


Trp Leu Gly Asp Glu Thr Lys Lys Lys Ser Met Phe Asp Ser Lys Arg 
65                  70                  75                  80  


Ala Ala Val Ala Leu Lys Trp Lys Gly Ile Glu Leu Cys Gly Val Ser 
                85                  90                  95      


Phe Asp Leu Leu Leu Ala Ala Tyr Leu Leu Asp Pro Ala Gln Gly Val 
            100                 105                 110         


Asp Asp Val Ala Ala Ala Ala Lys Met Lys Gln Tyr Glu Ala Val Arg 
        115                 120                 125             


Pro Asp Glu Ala Val Tyr Gly Lys Gly Ala Lys Arg Ala Val Pro Asp 
    130                 135                 140                 


Glu Pro Val Leu Ala Glu His Leu Val Arg Lys Ala Ala Ala Ile Trp 
145                 150                 155                 160 


Glu Leu Glu Arg Pro Phe Leu Asp Glu Leu Arg Arg Asn Glu Gln Asp 
                165                 170                 175     


Arg Leu Leu Val Glu Leu Glu Gln Pro Leu Ser Ser Ile Leu Ala Glu 
            180                 185                 190         


Met Glu Phe Ala Gly Val Lys Val Asp Thr Lys Arg Leu Glu Gln Met 
        195                 200                 205             


Gly Lys Glu Leu Ala Glu Gln Leu Gly Thr Val Glu Gln Arg Ile Tyr 
    210                 215                 220                 


Glu Leu Ala Gly Gln Glu Phe Asn Ile Asn Ser Pro Lys Gln Leu Gly 
225                 230                 235                 240 


Val Ile Leu Phe Glu Lys Leu Gln Leu Pro Val Leu Lys Lys Thr Lys 
                245                 250                 255     


Thr Gly Tyr Ser Thr Ser Ala Asp Val Leu Glu Lys Leu Ala Pro Tyr 
            260                 265                 270         


Arg Glu Ile Val Glu Asn Ile Leu Ala Tyr Arg Gln Leu Gly Lys Leu 
        275                 280                 285             


Gln Ser Thr Tyr Ile Glu Gly Leu Leu Lys Val Val Arg Pro Asp Thr 
    290                 295                 300                 


Lys Lys Val His Thr Ile Phe Asn Gln Ala Leu Thr Gln Thr Gly Arg 
305                 310                 315                 320 


Leu Ser Ser Thr Glu Pro Asn Leu Gln Asn Ile Pro Ile Arg Leu Glu 
                325                 330                 335     


Glu Gly Arg Lys Ile Arg Gln Ala Phe Val Pro Ser Glu Ser Asp Trp 
            340                 345                 350         


Leu Ile Phe Ala Ala Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala 
        355                 360                 365             


His Ile Ala Glu Asp Asp Asn Leu Met Glu Ala Phe Arg Arg Asp Leu 
    370                 375                 380                 


Asp Ile His Thr Lys Thr Ala Met Asp Ile Phe Gln Val Ser Glu Asp 
385                 390                 395                 400 


Glu Val Thr Pro Asn Met Arg Arg Gln Ala Lys Ala Val Asn Phe Gly 
                405                 410                 415     


Ile Val Tyr Gly Ile Ser Asp Tyr Gly Leu Ala Gln Asn Leu Asn Ile 
            420                 425                 430         


Ser Arg Lys Glu Ala Ala Glu Phe Ile Glu Arg Tyr Phe Gln Ser Phe 
        435                 440                 445             


Pro Gly Val Lys Arg Tyr Met Glu Asn Ile Val Gln Glu Ala Lys Gln 
    450                 455                 460                 


Lys Gly Tyr Val Thr Thr Leu Leu Arg Arg Arg Arg Phe Leu Pro Asp 
465                 470                 475                 480 


Ile Thr Ser Arg Asn Phe Asn Val Arg Ser Phe Ala Glu Arg Met Ala 
                485                 490                 495     


Met Asn Thr Pro Ile Gln Gly Ser Ala Ala Asp Ile Ile Lys Lys Ala 
            500                 505                 510         


Met Ile Asp Leu Asn Ala Arg Leu Lys Glu Glu Arg Leu Gln Ala Ala 
        515                 520                 525             


Leu Leu Leu Gln Val His Asp Glu Leu Ile Leu Glu Ala Pro Lys Glu 
    530                 535                 540                 


Glu Met Glu Arg Leu Cys Arg Leu Val Pro Glu Val Met Glu Gln Ala 
545                 550                 555                 560 


Val Thr Leu Arg Val Pro Leu Lys Val Asp Tyr Arg Tyr Gly Ser Thr 
                565                 570                 575     


Trp Tyr Asp Ala Lys 
            580     


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence for motif A family A polymerase


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  5

Asp Xaa Ser Xaa Xaa Glu 
1               5       


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence for motif B family A polymerase


<220>
<221>  misc_feature
<222>  (2)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  6

Lys Xaa Xaa Xaa Xaa Xaa Xaa Tyr Gly 
1               5                   


<210>  7
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence for motif C family A polymerase

<400>  7

Val His Asp Glu 
1               


<210>  8
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence for motif A family B polymerase


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  8

Asp Xaa Xaa Ser Leu Tyr Pro Ser 
1               5               


<210>  9
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence for motif B family B polymerase


<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  9

Lys Xaa Xaa Xaa Asn Ser Xaa Tyr Gly 
1               5                   


<210>  10
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence for motif C family B polymerase

<400>  10

Tyr Gly Asp Thr Asp Ser 
1               5       


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence for motif A


<220>
<221>  misc_feature
<222>  (2)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  11

Asp Xaa Xaa Xaa Xaa Phe Tyr 
1               5           


<210>  12
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence for motif B


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(6)
<223>  Xaa can be any naturally occurring amino acid

<400>  12

Phe Xaa Gly Xaa Xaa Xaa Ser Ala 
1               5               


<210>  13
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence for motif C


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  13

Tyr Xaa Asp Asp 
1               


<210>  14
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence for motif D


<220>
<221>  misc_feature
<222>  (2)..(8)
<223>  Xaa can be any naturally occurring amino acid

<400>  14

Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Lys 
1               5                   


<210>  15
<211>  832
<212>  PRT
<213>  Thermus aquaticus

<400>  15

Met Arg Gly Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe His Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Ala Val Ile Val 
    50                  55                  60                  


Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Gly Gly 
65                  70                  75                  80  


Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu 
                85                  90                  95      


Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Leu Ala Arg Leu Glu 
            100                 105                 110         


Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Ser Leu Ala Lys Lys 
        115                 120                 125             


Ala Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Lys Asp 
    130                 135                 140                 


Leu Tyr Gln Leu Leu Ser Asp Arg Ile His Val Leu His Pro Glu Gly 
145                 150                 155                 160 


Tyr Leu Ile Thr Pro Ala Trp Leu Trp Glu Lys Tyr Gly Leu Arg Pro 
                165                 170                 175     


Asp Gln Trp Ala Asp Tyr Arg Ala Leu Thr Gly Asp Glu Ser Asp Asn 
            180                 185                 190         


Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Arg Lys Leu Leu 
        195                 200                 205             


Glu Glu Trp Gly Ser Leu Glu Ala Leu Leu Lys Asn Leu Asp Arg Leu 
    210                 215                 220                 


Lys Pro Ala Ile Arg Glu Lys Ile Leu Ala His Met Asp Asp Leu Lys 
225                 230                 235                 240 


Leu Ser Trp Asp Leu Ala Lys Val Arg Thr Asp Leu Pro Leu Glu Val 
                245                 250                 255     


Asp Phe Ala Lys Arg Arg Glu Pro Asp Arg Glu Arg Leu Arg Ala Phe 
            260                 265                 270         


Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu 
        275                 280                 285             


Glu Ser Pro Lys Ala Leu Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly 
    290                 295                 300                 


Ala Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp 
305                 310                 315                 320 


Leu Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro 
                325                 330                 335     


Glu Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu 
            340                 345                 350         


Ala Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro 
        355                 360                 365             


Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn 
    370                 375                 380                 


Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu 
385                 390                 395                 400 


Glu Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu 
                405                 410                 415     


Trp Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu 
            420                 425                 430         


Val Glu Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly 
        435                 440                 445             


Val Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala 
    450                 455                 460                 


Glu Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His 
465                 470                 475                 480 


Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp 
                485                 490                 495     


Glu Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg 
            500                 505                 510         


Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile 
        515                 520                 525             


Val Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr 
    530                 535                 540                 


Tyr Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu 
545                 550                 555                 560 


His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser 
                565                 570                 575     


Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln 
            580                 585                 590         


Arg Ile Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala 
        595                 600                 605             


Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly 
    610                 615                 620                 


Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr 
625                 630                 635                 640 


Glu Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro 
                645                 650                 655     


Leu Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly 
            660                 665                 670         


Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu 
        675                 680                 685             


Ala Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg 
    690                 695                 700                 


Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val 
705                 710                 715                 720 


Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg 
                725                 730                 735     


Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro 
            740                 745                 750         


Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu 
        755                 760                 765             


Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His 
    770                 775                 780                 


Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala 
785                 790                 795                 800 


Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro 
                805                 810                 815     


Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830         


<210>  16
<211>  876
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  16

Met Lys Lys Lys Leu Val Leu Ile Asp Gly Ser Ser Val Ala Tyr Arg 
1               5                   10                  15      


Ala Phe Phe Ala Leu Pro Leu Leu His Asn Asp Lys Gly Ile His Thr 
            20                  25                  30          


Asn Ala Val Tyr Gly Phe Thr Met Met Leu Asn Lys Ile Leu Ala Glu 
        35                  40                  45              


Glu Glu Pro Thr His Met Leu Val Ala Phe Asp Ala Gly Lys Thr Thr 
    50                  55                  60                  


Phe Arg His Glu Ala Phe Gln Glu Tyr Lys Gly Gly Arg Gln Gln Thr 
65                  70                  75                  80  


Pro Pro Glu Leu Ser Glu Gln Phe Pro Leu Leu Arg Glu Leu Leu Arg 
                85                  90                  95      


Ala Tyr Arg Ile Pro Ala Tyr Glu Leu Glu Asn Tyr Glu Ala Asp Asp 
            100                 105                 110         


Ile Ile Gly Thr Leu Ala Ala Arg Ala Glu Gln Glu Gly Phe Glu Val 
        115                 120                 125             


Lys Val Ile Ser Gly Asp Arg Asp Leu Thr Gln Leu Ala Ser Pro His 
    130                 135                 140                 


Val Thr Val Asp Ile Thr Lys Lys Gly Ile Thr Asp Ile Glu Pro Tyr 
145                 150                 155                 160 


Thr Pro Glu Thr Val Arg Glu Lys Tyr Gly Leu Thr Pro Glu Gln Ile 
                165                 170                 175     


Val Asp Leu Lys Gly Leu Met Gly Asp Lys Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Pro Gly Ile Gly Glu Lys Thr Ala Val Lys Leu Leu Arg Gln Phe 
        195                 200                 205             


Gly Thr Val Glu Asn Val Leu Ala Ser Ile Asp Glu Ile Lys Gly Glu 
    210                 215                 220                 


Lys Leu Lys Glu Thr Leu Arg Gln His Arg Glu Met Ala Leu Leu Ser 
225                 230                 235                 240 


Lys Lys Leu Ala Ala Ile Arg Arg Asp Ala Pro Val Glu Leu Ser Leu 
                245                 250                 255     


Asp Asp Ile Ala Tyr Gln Gly Glu Asp Arg Glu Lys Val Val Ala Leu 
            260                 265                 270         


Phe Lys Glu Leu Gly Phe Gln Ser Phe Leu Glu Lys Met Glu Ser Pro 
        275                 280                 285             


Ser Ser Glu Glu Glu Lys Pro Leu Ala Lys Met Ala Phe Thr Leu Ala 
    290                 295                 300                 


Asp Arg Val Thr Glu Glu Met Leu Ala Asp Lys Ala Ala Leu Val Val 
305                 310                 315                 320 


Glu Val Val Glu Glu Asn Tyr His Asp Ala Pro Ile Val Gly Ile Ala 
                325                 330                 335     


Val Val Asn Glu His Gly Arg Phe Phe Leu Arg Pro Glu Thr Ala Leu 
            340                 345                 350         


Ala Asp Pro Gln Phe Val Ala Trp Leu Gly Asp Glu Thr Lys Lys Lys 
        355                 360                 365             


Ser Met Phe Asp Ser Lys Arg Ala Ala Val Ala Leu Lys Trp Lys Gly 
    370                 375                 380                 


Ile Glu Leu Cys Gly Val Ser Phe Asp Leu Leu Leu Ala Ala Tyr Leu 
385                 390                 395                 400 


Leu Asp Pro Ala Gln Gly Val Asp Asp Val Ala Ala Ala Ala Lys Met 
                405                 410                 415     


Lys Gln Tyr Glu Ala Val Arg Pro Asp Glu Ala Val Tyr Gly Lys Gly 
            420                 425                 430         


Ala Lys Arg Ala Val Pro Asp Glu Pro Val Leu Ala Glu His Leu Val 
        435                 440                 445             


Arg Lys Ala Ala Ala Ile Trp Glu Leu Glu Arg Pro Phe Leu Asp Glu 
    450                 455                 460                 


Leu Arg Arg Asn Glu Gln Asp Arg Leu Leu Val Glu Leu Glu Gln Pro 
465                 470                 475                 480 


Leu Ser Ser Ile Leu Ala Glu Met Glu Phe Ala Gly Val Lys Val Asp 
                485                 490                 495     


Thr Lys Arg Leu Glu Gln Met Gly Lys Glu Leu Ala Glu Gln Leu Gly 
            500                 505                 510         


Thr Val Glu Gln Arg Ile Tyr Glu Leu Ala Gly Gln Glu Phe Asn Ile 
        515                 520                 525             


Asn Ser Pro Lys Gln Leu Gly Val Ile Leu Phe Glu Lys Leu Gln Leu 
    530                 535                 540                 


Pro Val Leu Lys Lys Thr Lys Thr Gly Tyr Ser Thr Ser Ala Asp Val 
545                 550                 555                 560 


Leu Glu Lys Leu Ala Pro Tyr His Glu Ile Val Glu Asn Ile Leu His 
                565                 570                 575     


Tyr Arg Gln Leu Gly Lys Leu Gln Ser Thr Tyr Ile Glu Gly Leu Leu 
            580                 585                 590         


Lys Val Val Arg Pro Asp Thr Lys Lys Val His Thr Ile Phe Asn Gln 
        595                 600                 605             


Ala Leu Thr Gln Thr Gly Arg Leu Ser Ser Thr Glu Pro Asn Leu Gln 
    610                 615                 620                 


Asn Ile Pro Ile Arg Leu Glu Glu Gly Arg Lys Ile Arg Gln Ala Phe 
625                 630                 635                 640 


Val Pro Ser Glu Ser Asp Trp Leu Ile Phe Ala Ala Asp Tyr Ser Gln 
                645                 650                 655     


Ile Glu Leu Arg Val Leu Ala His Ile Ala Glu Asp Asp Asn Leu Met 
            660                 665                 670         


Glu Ala Phe Arg Arg Asp Leu Asp Ile His Thr Lys Thr Ala Met Asp 
        675                 680                 685             


Ile Phe Gln Val Ser Glu Asp Glu Val Thr Pro Asn Met Arg Arg Gln 
    690                 695                 700                 


Ala Lys Ala Val Asn Phe Gly Ile Val Tyr Gly Ile Ser Asp Tyr Gly 
705                 710                 715                 720 


Leu Ala Gln Asn Leu Asn Ile Ser Arg Lys Glu Ala Ala Glu Phe Ile 
                725                 730                 735     


Glu Arg Tyr Phe Glu Ser Phe Pro Gly Val Lys Arg Tyr Met Glu Asn 
            740                 745                 750         


Ile Val Gln Glu Ala Lys Gln Lys Gly Tyr Val Thr Thr Leu Leu His 
        755                 760                 765             


Arg Arg Arg Tyr Leu Pro Asp Ile Thr Ser Arg Asn Phe Asn Val Arg 
    770                 775                 780                 


Ser Phe Ala Glu Arg Met Ala Met Asn Thr Pro Ile Gln Gly Ser Ala 
785                 790                 795                 800 


Ala Asp Ile Ile Lys Lys Ala Met Ile Asp Leu Asn Ala Arg Leu Lys 
                805                 810                 815     


Glu Glu Arg Leu Gln Ala His Leu Leu Leu Gln Val His Asp Glu Leu 
            820                 825                 830         


Ile Leu Glu Ala Pro Lys Glu Glu Met Glu Arg Leu Cys Arg Leu Val 
        835                 840                 845             


Pro Glu Val Met Glu Gln Ala Val Thr Leu Arg Val Pro Leu Lys Val 
    850                 855                 860                 


Asp Tyr His Tyr Gly Ser Thr Trp Tyr Asp Ala Lys 
865                 870                 875     


<210>  17
<211>  1746
<212>  DNA
<213>  Bacillus stearothermophilus

<400>  17
atggcaaaaa tggcatttac cctggcagat cgtgttaccg aagaaatgct ggcagataaa       60

gcagcactgg ttgttgaagt tgtggaagaa aactatcatg atgcaccgat tgttggtatt      120

gccgttgtta atgaacgcgg tcgttttttt ctgcgtccgg aaaccgcact ggcagatccg      180

cagtttgttg catggctggg tgatgaaacc aaaaagaaaa gcatgttcga cagcaaacgt      240

gcagcagttg cactgaaatg gaaaggtatt gaactgtgcg gtgtgtcatt tgatctgctg      300

ctggcagcat atctgctgga tccggcacag ggtgttgatg atgttgcagc agcagcaaaa      360

atgaaacagt atgaagcagt tcgtccggat gaagcagttt atggtaaagg tgcaaaacgt      420

gcagttccgg atgaaccggt tctggcagaa catctggttc gtaaagcagc agcaatttgg      480

gaactggaac gtccgtttct ggatgaactg cgtcgtaatg aacaggatcg tctgctggtt      540

gaactggaac agccgctgag cagcattctg gcagaaatgg aatttgccgg tgttaaagtt      600

gataccaaac gtctggaaca aatgggtaaa gaactggccg aacaactggg caccgttgaa      660

cagcgtattt atgaactggc aggccaagaa tttaacatta atagcccgaa acagctgggc      720

gttatcctgt ttgaaaaact gcagctgccg gttctgaaaa aaaccaaaac cggttatagc      780

accagcgcag atgttctgga aaaactggca ccgtatcatg aaattgtgga aaacattctg      840

cactatcgtc agctgggtaa actgcagagc acctatattg aaggtctgct gaaagttgtg      900

cgtccggata ccaaaaaagt gcataccatt tttaaccagg cactgaccca gaccggtcgt      960

ctgagcagca ccgaaccgaa tctgcagaat attccgattc gtctggaaga aggtcgtaaa     1020

attcgtcagg catttgttcc gagcgaaagc gattggctga tttttgcagc agattatagc     1080

cagattgaac tgcgtgttct ggcacatatt gccgaagatg ataatctgat ggaagcattt     1140

cgtcgcgatc tggatattca taccaaaacc gccatggata tttttcaggt tagcgaagat     1200

gaagtgaccc cgaatatgcg tcgtcaggca aaagcagtta attttggtat tgtgtatggc     1260

atcagcgatt atggtctggc acagaatctg aatattagcc gtaaagaagc agccgaattt     1320

atcgaacgtt actttcagag ctttccgggt gttaaacgct atatggaaaa cattgtccaa     1380

gaagccaaac agaaaggtta tgttaccacc ctgctgcatc gtcgtcgtta tctgccgcgt     1440

attaccagcc gtaactttaa tgttcgtagc tttgcagaac gcatggcaat gaataccccg     1500

attcagggta gcgcagcaga tattatcaaa aaagccatga tcgatctgaa cgcacgtctg     1560

aaagaagaac gtctgcaggc acatttactg ctgcaggttc atgatgaact gattctggaa     1620

gcaccgaaag aagaaatgga acgtctttgt cgtctggttc cggaagttat ggaacaggca     1680

gttaccctgc gtgttccgct gaaagttgat tatcgttatg gtagcacctg gtatgatgcc     1740

aaataa                                                                1746


<210>  18
<211>  606
<212>  PRT
<213>  Escherichia coli

<400>  18

Met Val Ile Ser Tyr Asp Asn Tyr Val Thr Ile Leu Asp Glu Glu Thr 
1               5                   10                  15      


Leu Lys Ala Trp Ile Ala Lys Leu Glu Lys Ala Pro Val Phe Ala Phe 
            20                  25                  30          


Asp Thr Glu Thr Asp Ser Leu Asp Asn Ile Ser Ala Asn Leu Val Gly 
        35                  40                  45              


Leu Ser Phe Ala Ile Glu Pro Gly Val Ala Ala Tyr Ile Pro Val Ala 
    50                  55                  60                  


His Asp Tyr Leu Asp Ala Pro Asp Gln Ile Ser Arg Glu Arg Ala Leu 
65                  70                  75                  80  


Glu Leu Leu Lys Pro Leu Leu Glu Asp Glu Lys Ala Leu Lys Val Gly 
                85                  90                  95      


Gln Asn Leu Lys Tyr Asp Arg Gly Ile Leu Ala Asn Tyr Gly Ile Glu 
            100                 105                 110         


Leu Arg Gly Ile Ala Phe Asp Thr Met Leu Glu Ser Tyr Ile Leu Asn 
        115                 120                 125             


Ser Val Ala Gly Arg His Asp Met Asp Ser Leu Ala Glu Arg Trp Leu 
    130                 135                 140                 


Lys His Lys Thr Ile Thr Phe Glu Glu Ile Ala Gly Lys Gly Lys Asn 
145                 150                 155                 160 


Gln Leu Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Gly Arg Tyr Ala 
                165                 170                 175     


Ala Glu Asp Ala Asp Val Thr Leu Gln Leu His Leu Lys Met Trp Pro 
            180                 185                 190         


Asp Leu Gln Lys His Lys Gly Pro Leu Asn Val Phe Glu Asn Ile Glu 
        195                 200                 205             


Met Pro Leu Val Pro Val Leu Ser Arg Ile Glu Arg Asn Gly Val Lys 
    210                 215                 220                 


Ile Asp Pro Lys Val Leu His Asn His Ser Glu Glu Leu Thr Leu Arg 
225                 230                 235                 240 


Leu Ala Glu Leu Glu Lys Lys Ala His Glu Ile Ala Gly Glu Glu Phe 
                245                 250                 255     


Asn Leu Ser Ser Thr Lys Gln Leu Gln Thr Ile Leu Phe Glu Lys Gln 
            260                 265                 270         


Gly Ile Lys Pro Leu Lys Lys Thr Pro Gly Gly Ala Pro Ser Thr Ser 
        275                 280                 285             


Glu Glu Val Leu Glu Glu Leu Ala Leu Asp Tyr Pro Leu Pro Lys Val 
    290                 295                 300                 


Ile Leu Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr Thr Asp 
305                 310                 315                 320 


Lys Leu Pro Leu Met Ile Asn Pro Lys Thr Gly Arg Val His Thr Ser 
                325                 330                 335     


Tyr His Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr Asp Pro 
            340                 345                 350         


Asn Leu Gln Asn Ile Pro Val Arg Asn Glu Glu Gly Arg Arg Ile Arg 
        355                 360                 365             


Gln Ala Phe Ile Ala Pro Glu Asp Tyr Val Ile Val Ser Ala Asp Tyr 
    370                 375                 380                 


Ser Gln Ile Glu Leu Arg Ile Met Ala His Leu Ser Arg Asp Lys Gly 
385                 390                 395                 400 


Leu Leu Thr Ala Phe Ala Glu Gly Lys Asp Ile His Arg Ala Thr Ala 
                405                 410                 415     


Ala Glu Val Phe Gly Leu Pro Leu Glu Thr Val Thr Ser Glu Gln Arg 
            420                 425                 430         


Arg Ser Ala Lys Ala Ile Asn Phe Gly Leu Ile Tyr Gly Met Ser Ala 
        435                 440                 445             


Phe Gly Leu Ala Arg Gln Leu Asn Ile Pro Arg Lys Glu Ala Gln Lys 
    450                 455                 460                 


Tyr Met Asp Leu Tyr Phe Glu Arg Tyr Pro Gly Val Leu Glu Tyr Met 
465                 470                 475                 480 


Glu Arg Thr Arg Ala Gln Ala Lys Glu Gln Gly Tyr Val Glu Thr Leu 
                485                 490                 495     


Asp Gly Arg Arg Leu Tyr Leu Pro Asp Ile Lys Ser Ser Asn Gly Ala 
            500                 505                 510         


Arg Arg Ala Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met Gln Gly 
        515                 520                 525             


Thr Ala Ala Asp Ile Ile Lys Arg Ala Met Ile Ala Val Asp Ala Trp 
    530                 535                 540                 


Leu Gln Ala Glu Gln Pro Arg Val Arg Met Ile Met Gln Val His Asp 
545                 550                 555                 560 


Glu Leu Val Phe Glu Val His Lys Asp Asp Val Asp Ala Val Ala Lys 
                565                 570                 575     


Gln Ile His Gln Leu Met Glu Asn Cys Thr Arg Leu Asp Val Pro Leu 
            580                 585                 590         


Leu Val Glu Val Gly Ser Gly Glu Asn Trp Asp Gln Ala His 
        595                 600                 605     


<210>  19
<211>  581
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  19

Met Ala Lys Met Ala Phe Thr Leu Ala Asp Arg Val Thr Glu Glu Met 
1               5                   10                  15      


Leu Ala Asp Lys Ala Ala Leu Val Val Glu Val Val Glu Glu Asn Tyr 
            20                  25                  30          


His Asp Ala Pro Ile Val Gly Ile Ala Val Val Asn Glu Arg Gly Arg 
        35                  40                  45              


Phe Phe Leu Arg Pro Glu Thr Ala Leu Ala Asp Pro Gln Phe Val Ala 
    50                  55                  60                  


Trp Leu Gly Asp Glu Thr Lys Lys Lys Ser Met Phe Asp Ser Lys Arg 
65                  70                  75                  80  


Ala Ala Val Ala Leu Lys Trp Lys Gly Ile Glu Leu Cys Gly Val Ser 
                85                  90                  95      


Phe Asp Leu Leu Leu Ala Ala Tyr Leu Leu Asp Pro Ala Gln Gly Val 
            100                 105                 110         


Asp Asp Val Ala Ala Ala Ala Lys Met Lys Gln Tyr Glu Ala Val Arg 
        115                 120                 125             


Pro Asp Glu Ala Val Tyr Gly Lys Gly Ala Lys Arg Ala Val Pro Asp 
    130                 135                 140                 


Glu Pro Val Leu Ala Glu His Leu Val Arg Lys Ala Ala Ala Ile Trp 
145                 150                 155                 160 


Glu Leu Glu Arg Pro Phe Leu Asp Glu Leu Arg Arg Asn Glu Gln Asp 
                165                 170                 175     


Arg Leu Leu Val Glu Leu Glu Gln Pro Leu Ser Ser Ile Leu Ala Glu 
            180                 185                 190         


Met Glu Phe Ala Gly Val Lys Val Asp Thr Lys Arg Leu Glu Gln Met 
        195                 200                 205             


Gly Lys Glu Leu Ala Glu Gln Leu Gly Thr Val Lys Gln Arg Ile Tyr 
    210                 215                 220                 


Glu Leu Ala Gly Gln Glu Phe Asn Ile Arg Ser Pro Lys Gln Leu Gly 
225                 230                 235                 240 


Val Ile Leu Phe Glu Lys Leu Gln Leu Pro Val Leu Lys Lys Thr Lys 
                245                 250                 255     


Thr Gly Tyr Ser Thr Ser Ala Asp Val Leu Glu Lys Leu Ala Pro Tyr 
            260                 265                 270         


His Glu Ile Val Glu Asn Ile Leu His Tyr Arg Gln Leu Gly Lys Leu 
        275                 280                 285             


Gln Ser Thr Tyr Ile Glu Gly Leu Leu Lys Val Val Arg Pro Asp Thr 
    290                 295                 300                 


Lys Lys Val His Thr Ile Phe Asn Gln Ala Leu Thr Gln Thr Gly Arg 
305                 310                 315                 320 


Leu Ser Ser Thr Glu Pro Asn Leu Gln Asn Ile Pro Ile Arg Leu Glu 
                325                 330                 335     


Glu Gly Arg Lys Ile Arg Gln Ala Phe Val Pro Ser Glu Ser Asp Trp 
            340                 345                 350         


Leu Ile Phe Ala Ala Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala 
        355                 360                 365             


His Ile Ala Glu Asp Asp Asn Leu Met Glu Ala Phe Arg Arg Asp Leu 
    370                 375                 380                 


Asp Ile His Thr Lys Thr Ala Met Asp Ile Phe Gln Val Ser Glu Asp 
385                 390                 395                 400 


Glu Val Thr Pro Asn Met Arg Arg Gln Ala Lys Ala Val Asn Phe Gly 
                405                 410                 415     


Ile Val Tyr Gly Ile Ser Lys Tyr Gly Leu Ala Gln Asn Leu Asn Ile 
            420                 425                 430         


Ser Arg Lys Glu Ala Ala Glu Phe Ile Glu Arg Tyr Phe Gln Ser Phe 
        435                 440                 445             


Pro Gly Val Lys Arg Tyr Met Glu Asn Ile Val Gln Glu Ala Lys Gln 
    450                 455                 460                 


Lys Gly Tyr Val Thr Thr Leu Leu His Arg Arg Arg Tyr Leu Pro Asp 
465                 470                 475                 480 


Ile Thr Ser Arg Asn Phe Asn Val Arg Ser Phe Ala Glu Arg Met Ala 
                485                 490                 495     


Met Asn Thr Pro Ile Gln Gly Ser Ala Ala Asp Ile Ile Lys Lys Ala 
            500                 505                 510         


Met Ile Asp Leu Asn Ala Arg Leu Lys Glu Glu Arg Leu Gln Ala His 
        515                 520                 525             


Leu Leu Leu Gln Val His Asp Glu Leu Ile Leu Glu Ala Pro Lys Glu 
    530                 535                 540                 


Glu Met Glu Arg Leu Cys Arg Leu Val Pro Glu Val Met Glu Gln Ala 
545                 550                 555                 560 


Val Thr Leu Arg Val Pro Leu Lys Val Asp Tyr Arg Tyr Gly Ser Thr 
                565                 570                 575     


Trp Tyr Asp Ala Lys 
            580     


<210>  20
<211>  581
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  20

Met Ala Lys Met Ala Phe Thr Leu Ala Asp Arg Val Thr Glu Glu Met 
1               5                   10                  15      


Leu Ala Asp Lys Ala Ala Leu Val Val Glu Val Val Glu Glu Asn Tyr 
            20                  25                  30          


His Asp Ala Pro Ile Val Gly Ile Ala Val Val Asn Glu Arg Gly Arg 
        35                  40                  45              


Phe Phe Leu Arg Pro Glu Thr Ala Leu Ala Asp Pro Gln Phe Val Ala 
    50                  55                  60                  


Trp Leu Gly Asp Glu Thr Lys Lys Lys Ser Met Phe Asp Ser Lys Arg 
65                  70                  75                  80  


Ala Ala Val Ala Leu Lys Trp Lys Gly Ile Glu Leu Cys Gly Val Ser 
                85                  90                  95      


Phe Asp Leu Leu Leu Ala Ala Tyr Leu Leu Asp Pro Ala Gln Gly Val 
            100                 105                 110         


Asp Asp Val Ala Ala Ala Ala Lys Met Lys Gln Tyr Glu Ala Val Arg 
        115                 120                 125             


Pro Asp Glu Ala Val Tyr Gly Lys Gly Ala Lys Arg Ala Val Pro Asp 
    130                 135                 140                 


Glu Pro Val Leu Ala Glu His Leu Val Arg Lys Ala Ala Ala Ile Trp 
145                 150                 155                 160 


Glu Leu Glu Arg Pro Phe Leu Asp Glu Leu Arg Arg Asn Glu Gln Asp 
                165                 170                 175     


Arg Leu Leu Val Glu Leu Glu Gln Pro Leu Ser Ser Ile Leu Ala Glu 
            180                 185                 190         


Met Glu Phe Ala Gly Val Lys Val Asp Thr Lys Arg Leu Glu Gln Met 
        195                 200                 205             


Gly Lys Glu Leu Ala Glu Gln Leu Gly Thr Val Lys Gln Arg Ile Tyr 
    210                 215                 220                 


Glu Leu Ala Gly Gln Glu Phe Asn Ile Arg Ser Pro Lys Gln Leu Gly 
225                 230                 235                 240 


Val Ile Leu Phe Glu Lys Leu Gln Leu Pro Val Leu Lys Lys Thr Lys 
                245                 250                 255     


Thr Gly Tyr Ser Thr Ser Ala Asp Val Leu Glu Lys Leu Ala Pro Tyr 
            260                 265                 270         


His Glu Ile Val Glu Asn Ile Leu His Tyr Arg Gln Leu Gly Lys Leu 
        275                 280                 285             


Gln Ser Thr Tyr Ile Glu Gly Leu Leu Lys Val Val Arg Pro Asp Thr 
    290                 295                 300                 


Lys Lys Val His Thr Ile Phe Asn Gln Ala Leu Thr Gln Thr Gly Arg 
305                 310                 315                 320 


Leu Ser Ser Thr Glu Pro Asn Leu Gln Asn Ile Pro Ile Arg Leu Glu 
                325                 330                 335     


Glu Gly Arg Lys Ile Arg Gln Ala Phe Val Pro Ser Glu Ser Asp Trp 
            340                 345                 350         


Leu Ile Phe Ala Ala Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala 
        355                 360                 365             


His Ile Ala Glu Asp Asp Asn Leu Met Glu Ala Phe Arg Arg Asp Leu 
    370                 375                 380                 


Asp Ile His Thr Lys Thr Ala Met Asp Ile Phe Gln Val Ser Glu Asp 
385                 390                 395                 400 


Glu Val Thr Pro Asn Met Arg Arg Gln Ala Lys Ala Val Asn Phe Gly 
                405                 410                 415     


Ile Val Tyr Gly Ile Ser Lys Tyr Gly Leu Ala Gln Asn Leu Asn Ile 
            420                 425                 430         


Ser Arg Lys Glu Ala Ala Glu Phe Ile Glu Arg Tyr Phe Gln Ser Phe 
        435                 440                 445             


Pro Gly Val Lys Arg Tyr Met Glu Asn Ile Val Gln Glu Ala Lys Gln 
    450                 455                 460                 


Lys Gly Tyr Val Thr Thr Leu Leu Arg Arg Arg Arg Tyr Leu Pro Asp 
465                 470                 475                 480 


Ile Thr Ser Arg Asn Phe Asn Val Arg Ser Phe Ala Glu Arg Met Ala 
                485                 490                 495     


Met Asn Thr Pro Ile Gln Gly Ser Ala Ala Asp Ile Ile Lys Lys Ala 
            500                 505                 510         


Met Ile Asp Leu Asn Ala Arg Leu Lys Glu Glu Arg Leu Gln Ala Ala 
        515                 520                 525             


Leu Leu Leu Gln Val His Asp Glu Leu Ile Leu Glu Ala Pro Lys Glu 
    530                 535                 540                 


Glu Met Glu Arg Leu Cys Arg Leu Val Pro Glu Val Met Glu Gln Ala 
545                 550                 555                 560 


Val Thr Leu Arg Val Pro Leu Lys Val Asp Tyr Arg Tyr Gly Ser Thr 
                565                 570                 575     


Trp Tyr Asp Ala Lys 
            580     


<210>  21
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA oligonucleotide


<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  N = fluorescein-dT

<400>  21
tttttttgca ggtgacaggt ttttcctgtc accngc                                 36


<210>  22
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA oligonucleotide

<400>  22
tttttttccc tttcctttcg ggtgacaggt ttttcctgtc accc                        44


<210>  23
<211>  1743
<212>  DNA
<213>  Bacillus stearothermophilus

<400>  23
atggctaaaa tggcttttac tcttgctgat cgtgttactg aagaaatgct tgctgataaa       60

gctgctcttg ttgttgaagt tgttgaagaa aattatcatg atgctcctat tgttggtatt      120

gctgttgtta atgaacgtgg tcgttttttt cttcgtcctg aaactgctct tgctgatcct      180

caatttgttg cttggcttgg tgatgaaact aaaaaaaaat ctatgtttga ttctaaacgt      240

gctgctgttg ctcttaaatg gaaaggtatt gaactttgtg gtgtttcttt tgatcttctt      300

cttgctgctt atcttcttga tcctgctcaa ggtgttgatg atgttgctgc tgctgctaaa      360

atgaaacaat atgaagctgt tcgtcctgat gaagctgttt atggtaaagg tgctaaacgt      420

gctgttcctg atgaacctgt tcttgctgaa catcttgttc gtaaagctgc tgctatttgg      480

gaacttgaac gtccttttct tgatgaactt cgtcgtaatg aacaagatcg tcttcttgtt      540

gaacttgaac aacctctttc ttctattctt gctgaaatgg aatttgctgg tgttaaagtt      600

gatactaaac gtcttgaaca aatgggtaaa gaacttgctg aacaacttgg tactgttaaa      660

caacgtattt atgaacttgc tggtcaagaa tttaatattc gttctcctaa acaacttggt      720

gttattcttt ttgaaaaact tcaacttcct gttcttaaaa aaactaaaac tggttattct      780

acttctgctg atgttcttga aaaacttgct ccttatcatg aaattgttga aaatattctt      840

cattatcgtc aacttggtaa acttcaatct acttatattg aaggtcttct taaagttgtt      900

cgtcctgata ctaaaaaagt tcatactatt tttaatcaag ctcttactca aactggtcgt      960

ctttcttcta ctgaacctaa tcttcaaaat attcctattc gtcttgaaga aggtcgtaaa     1020

attcgtcaag cttttgttcc ttctgaatct gattggctta tttttgctgc tgattattct     1080

caaattgaac ttcgtgttct tgctcatatt gctgaagatg ataatcttat ggaagctttt     1140

cgtcgtgatc ttgatattca tactaaaact gctatggata tttttcaagt ttctgaagat     1200

gaagttactc ctaatatgcg tcgtcaagct aaagctgtta attttggtat tgtttatggt     1260

atttctaaat atggtcttgc tcaaaatctt aatatttctc gtaaagaagc tgctgaattt     1320

attgaacgtt attttcaatc ttttcctggt gttaaacgtt atatggaaaa tattgttcaa     1380

gaagctaaac aaaaaggtta tgttactact cttcttcatc gtcgtcgtta tcttcctgat     1440

attacttctc gtaattttaa tgttcgttct tttgctgaac gtatggctat gaatactcct     1500

attcaaggtt ctgctgctga tattattaaa aaagctatga ttgatcttaa tgctcgtctt     1560

aaagaagaac gtcttcaagc tcatcttctt cttcaagttc atgatgaact tattcttgaa     1620

gctcctaaag aagaaatgga acgtctttgt cgtcttgttc ctgaagttat ggaacaagct     1680

gttactcttc gtgttcctct taaagttgat tatcgttatg gttctacttg gtatgatgct     1740

aaa                                                                   1743


<210>  24
<211>  832
<212>  PRT
<213>  Thermus aquaticus

<400>  24

Met Arg Gly Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      


Val Asp Gly His His Leu Ala Tyr Arg Thr Phe His Ala Leu Lys Gly 
            20                  25                  30          


Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              


Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Ala Val Ile Val 
    50                  55                  60                  


Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Gly Gly 
65                  70                  75                  80  


Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu 
                85                  90                  95      


Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Leu Ala Arg Leu Glu 
            100                 105                 110         


Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Ser Leu Ala Lys Lys 
        115                 120                 125             


Ala Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Lys Asp 
    130                 135                 140                 


Leu Tyr Gln Leu Leu Ser Asp Arg Ile His Val Leu His Pro Glu Gly 
145                 150                 155                 160 


Tyr Leu Ile Thr Pro Ala Trp Leu Trp Glu Lys Tyr Gly Leu Arg Pro 
                165                 170                 175     


Asp Gln Trp Ala Asp Tyr Arg Ala Leu Thr Gly Asp Glu Ser Asp Asn 
            180                 185                 190         


Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Arg Lys Leu Leu 
        195                 200                 205             


Glu Glu Trp Gly Ser Leu Glu Ala Leu Leu Lys Asn Leu Asp Arg Leu 
    210                 215                 220                 


Lys Pro Ala Ile Arg Glu Lys Ile Leu Ala His Met Asp Asp Leu Lys 
225                 230                 235                 240 


Leu Ser Trp Asp Leu Ala Lys Val Arg Thr Asp Leu Pro Leu Glu Val 
                245                 250                 255     


Asp Phe Ala Lys Arg Arg Glu Pro Asp Arg Glu Arg Leu Arg Ala Phe 
            260                 265                 270         


Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu 
        275                 280                 285             


Glu Ser Pro Lys Ala Leu Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly 
    290                 295                 300                 


Ala Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp 
305                 310                 315                 320 


Leu Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro 
                325                 330                 335     


Glu Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu 
            340                 345                 350         


Ala Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro 
        355                 360                 365             


Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn 
    370                 375                 380                 


Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu 
385                 390                 395                 400 


Glu Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu 
                405                 410                 415     


Trp Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu 
            420                 425                 430         


Val Glu Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly 
        435                 440                 445             


Val Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala 
    450                 455                 460                 


Glu Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His 
465                 470                 475                 480 


Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp 
                485                 490                 495     


Glu Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg 
            500                 505                 510         


Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile 
        515                 520                 525             


Val Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr 
    530                 535                 540                 


Tyr Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu 
545                 550                 555                 560 


His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser 
                565                 570                 575     


Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln 
            580                 585                 590         


Arg Ile Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala 
        595                 600                 605             


Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly 
    610                 615                 620                 


Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr 
625                 630                 635                 640 


Glu Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro 
                645                 650                 655     


Leu Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly 
            660                 665                 670         


Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu 
        675                 680                 685             


Ala Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg 
    690                 695                 700                 


Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val 
705                 710                 715                 720 


Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Arg Leu Glu Ala Arg 
                725                 730                 735     


Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro 
            740                 745                 750         


Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu 
        755                 760                 765             


Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His 
    770                 775                 780                 


Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala 
785                 790                 795                 800 


Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro 
                805                 810                 815     


Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830         


