                         SEQUENCE LISTING

<110>  E.I. duPont de Nemours & Company, Inc.
       Odom, J. Martin
       Orlandi, Robert
 
<120>  IMPROVED AQUACULTURE FEED COMPOSITIONS

<130>  CL5143USCIP2

<150>  US 61/441,836
<151>  2011-02-11

<150>  US 12/854,449
<151>  2010-08-11

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  4313
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKUM

<400>  1
taatcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc       60

acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga      120

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg      180

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg      240

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg      300

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga      360

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg      420

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag      480

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc      540

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg      600

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt      660

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc      720

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc      780

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg      840

tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca      900

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc      960

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat     1020

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt     1080

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt     1140

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc     1200

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc     1260

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata     1320

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg     1380

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc     1440

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct     1500

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa     1560

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt     1620

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca     1680

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac     1740

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca     1800

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt     1860

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc     1920

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca     1980

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata     2040

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc     2100

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc     2160

cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt     2220

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc     2280

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct     2340

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat     2400

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc     2460

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc     2520

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg     2580

atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc     2640

cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc     2700

agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc     2760

agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat     2820

tgggtaccgg gccccccctc gaggtcgacg agtatctgtc tgactcgtca ttgccgcctt     2880

tggagtacga ctccaactat gagtgtgctt ggatcacttt gacgatacat tcttcgttgg     2940

aggctgtggg tctgacagct gcgttttcgg cgcggttggc cgacaacaat atcagctgca     3000

acgtcattgc tggctttcat catgatcaca tttttgtcgg caaaggcgac gcccagagag     3060

ccattgacgt tctttctaat ttggaccgat agccgtatag tccagtctat ctataagttc     3120

aactaactcg taactattac cataacatat acttcactgc cccagataag gttccgataa     3180

aaagttctgc agactaaatt tatttcagtc tcctcttcac caccaaaatg ccctcctacg     3240

aagctcgagt gctcaagctc gtggcagcca agaaaaccaa cctgtgtgct tctctggatg     3300

ttaccaccac caaggagctc attgagcttg ccgataaggt cggaccttat gtgtgcatga     3360

tcaaaaccca tatcgacatc attgacgact tcacctacgc cggcactgtg ctccccctca     3420

aggaacttgc tcttaagcac ggtttcttcc tgttcgagga cagaaagttc gcagatattg     3480

gcaacactgt caagcaccag taccggtgtc accgaatcgc cgagtggtcc gatatcacca     3540

acgcccacgg tgtacccgga accggaatcg attgctggcc tgcgagctgg tgcgtacgag     3600

gaaactgtct ctgaacagaa gaaggaggac gtctctgact acgagaactc ccagtacaag     3660

gagttcctag tcccctctcc caacgagaag ctggccagag gtctgctcat gctggccgag     3720

ctgtcttgca agggctctct ggccactggc gagtactcca agcagaccat tgagcttgcc     3780

cgatccgacc ccgagtttgt ggttggcttc attgcccaga accgacctaa gggcgactct     3840

gaggactggc ttattctgac ccccggggtg ggtcttgacg acaagggaga cgctctcgga     3900

cagcagtacc gaactgttga ggatgtcatg tctaccggaa cggatatcat aattgtcggc     3960

cgaggtctgt acggccagaa ccgagatcct attgaggagg ccaagcgata ccagaaggct     4020

ggctgggagg cttaccagaa gattaactgt tagaggttag actatggata tgtaatttaa     4080

ctgtgtatat agagagcgtg caagtatgga gcgcttgttc agcttgtatg atggtcagac     4140

gacctgtctg atcgagtatg tatgatactg cacaacctgt gtatccgcat gatctgtcca     4200

atggggcatg ttgttgtgtt tctcgatacg gagatgctgg gtacagtgct aatacgttga     4260

actacttata cttatatgag gctcgaagaa agctgacttg tgtatgactt aat            4313


<210>  2
<211>  13565
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKL3-9DP9N

<400>  2
gtacggattg tgtatgtccc tgtacctgca tcttgatgga gagagctccg gaaagcggat       60

caggagctgt ccaattttaa ttttataaca tggaaacgag tccttggagc tagaagacca      120

ttttttcaac tgccctatcg actatattta tctactccaa aaccgactgc ttcccaagaa      180

tcttcagcca aggcttccaa agtaacccct cgcttcccga cacttaattg aaaccttaga      240

tgcagtcact gcgagtgaag tggactctaa catctccaac atagcgacga tattgcgagg      300

gtttgaatat aactaagatg catgatccat tacatttgta gaaatatcat aaacaacgaa      360

gcacatagac agaatgctgt tggttgttac atctgaagcc gaggtaccga tgtcattttc      420

agctgtcact gcagagacag gggtatgtca catttgaaga tcatacaacc gacgtttatg      480

aaaaccagag atatagagaa tgtattgacg gttgtggcta tgtcataagt gcagtgaagt      540

gcagtgatta taggtatagt acacttactg tagctacaag tacatactgc tacagtaata      600

ctcatgtatg caaaccgtat tctgtgtcta cagaaggcga tacggaagag tcaatctctt      660

atgtagagcc atttctataa tcgaaggggc cttgtaattt ccaaacgagt aattgagtaa      720

ttgaagagca tcgtagacat tacttatcat gtattgtgag agggaggaga tgcagctgta      780

gctactgcac atactgtact cgcccatgca gggataatgc atagcgagac ttggcagtag      840

gtgacagttg ctagctgcta cttgtagtcg ggtgggtgat agcatggcgc gccagctgca      900

ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc      960

ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc     1020

aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc     1080

aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag     1140

gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc     1200

gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt     1260

tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct     1320

ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg     1380

ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct     1440

tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat     1500

tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg     1560

ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa     1620

aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt     1680

ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc     1740

tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt     1800

atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta     1860

aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat     1920

ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac     1980

tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg     2040

ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag     2100

tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt     2160

aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt     2220

gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt     2280

tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt     2340

cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct     2400

tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt     2460

ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac     2520

cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa     2580

actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa     2640

ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca     2700

aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct     2760

ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga     2820

atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc     2880

tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt     2940

aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa     3000

ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt     3060

gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa     3120

agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag     3180

ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt     3240

tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg     3300

agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc     3360

cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg     3420

gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct     3480

gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg     3540

gccagtgaat tgtaatacga ctcactatag ggcgaattgg gcccgacgtc gcatgcagga     3600

atagacatct tcaataggag cattaatacc tgtgggatca ctgatgtaaa cttctcccag     3660

agtatgtgaa taaccagcgg gccatccaac aaagaagtcg ttccagtgag tgactcggta     3720

catccgtctt tcggggttga tggtaagtcc gtcgtctcct tgcttaaaga acagagcgtc     3780

cacgtagtct gcaaaagcct tgtttccaag tcgaggctgc ccatagttga ttagcgttgg     3840

atcatatcca agattcttca ggttgatgcc catgaataga gcagtgacag ctcctagaga     3900

gtggccagtt acgatcaatt tgtagtcagt gttgtttcca aggaagtcga ccagacgatc     3960

ctgtacgttc accatagtct ctctgtatgc cttctgaaag ccatcatgaa cttggcagcc     4020

aggacaattg atactggcag aagggtttgt ggagtttatg tcagtagtgt taagaggagg     4080

gatactggtc atgtagggtt gttggatcgt ttggatgtca gtaatagcgt ctgcaatgga     4140

gaaagtgcct cggaaaacaa tatacttttc ctttttggtg tgatcgtggg ccaaaaatcc     4200

agtaactgaa gtcgagaaga aatttcctcc aaactggtag tcaagagtca catcgggaaa     4260

atgagcgcaa gagtttccac aggtaaaatc gctctgcagg gcaaatgggc caggggctct     4320

gacacaatag gccacgttag atagccatcc gtacttgaga acaaagtcgt atgtctcctg     4380

ggtgatagga gccgttaatt aagttgcgac acatgtcttg atagtatctt gaattctctc     4440

tcttgagctt ttccataaca agttcttctg cctccaggaa gtccatgggt ggtttgatca     4500

tggttttggt gtagtggtag tgcagtggtg gtattgtgac tggggatgta gttgagaata     4560

agtcatacac aagtcagctt tcttcgagcc tcatataagt ataagtagtt caacgtatta     4620

gcactgtacc cagcatctcc gtatcgagaa acacaacaac atgccccatt ggacagatca     4680

tgcggataca caggttgtgc agtatcatac atactcgatc agacaggtcg tctgaccatc     4740

atacaagctg aacaagcgct ccatacttgc acgctctcta tatacacagt taaattacat     4800

atccatagtc taacctctaa cagttaatct tctggtaagc ctcccagcca gccttctggt     4860

atcgcttggc ctcctcaata ggatctcggt tctggccgta cagacctcgg ccgacaatta     4920

tgatatccgt tccggtagac atgacatcct caacagttcg gtactgctgt ccgagagcgt     4980

ctcccttgtc gtcaagaccc accccggggg tcagaataag ccagtcctca gagtcgccct     5040

taggtcggtt ctgggcaatg aagccaacca caaactcggg gtcggatcgg gcaagctcaa     5100

tggtctgctt ggagtactcg ccagtggcca gagagccctt gcaagacagc tcggccagca     5160

tgagcagacc tctggccagc ttctcgttgg gagaggggac taggaactcc ttgtactggg     5220

agttctcgta gtcagagacg tcctccttct tctgttcaga gacagtttcc tcggcaccag     5280

ctcgcaggcc agcaatgatt ccggttccgg gtacaccgtg ggcgttggtg atatcggacc     5340

actcggcgat tcggtgacac cggtactggt gcttgacagt gttgccaata tctgcgaact     5400

ttctgtcctc gaacaggaag aaaccgtgct taagagcaag ttccttgagg gggagcacag     5460

tgccggcgta ggtgaagtcg tcaatgatgt cgatatgggt tttgatcatg cacacataag     5520

gtccgacctt atcggcaagc tcaatgagct ccttggtggt ggtaacatcc agagaagcac     5580

acaggttggt tttcttggct gccacgagct tgagcactcg agcggcaaag gcggacttgt     5640

ggacgttagc tcgagcttcg taggagggca ttttggtggt gaagaggaga ctgaaataaa     5700

tttagtctgc agaacttttt atcggaacct tatctggggc agtgaagtat atgttatggt     5760

aatagttacg agttagttga acttatagat agactggact atacggctat cggtccaaat     5820

tagaaagaac gtcaatggct ctctgggcgt cgcctttgcc gacaaaaatg tgatcatgat     5880

gaaagccagc aatgacgttg cagctgatat tgttgtcggc caaccgcgcc gaaaacgcag     5940

ctgtcagacc cacagcctcc aacgaagaat gtatcgtcaa agtgatccaa gcacactcat     6000

agttggagtc gtactccaaa ggcggcaatg acgagtcaga cagatactcg tcgacctttt     6060

ccttgggaac caccaccgtc agcccttctg actcacgtat tgtagccacc gacacaggca     6120

acagtccgtg gatagcagaa tatgtcttgt cggtccattt ctcaccaact ttaggcgtca     6180

agtgaatgtt gcagaagaag tatgtgcctt cattgagaat cggtgttgct gatttcaata     6240

aagtcttgag atcagtttgg ccagtcatgt tgtggggggt aattggattg agttatcgcc     6300

tacagtctgt acaggtatac tcgctgccca ctttatactt tttgattccg ctgcacttga     6360

agcaatgtcg tttaccaaaa gtgagaatgc tccacagaac acaccccagg gtatggttga     6420

gcaaaaaata aacactccga tacggggaat cgaaccccgg tctccacggt tctcaagaag     6480

tattcttgat gagagcgtat cgatggttaa tgctgctgtg tgctgtgtgt gtgtgttgtt     6540

tggcgctcat tgttgcgtta tgcagcgtac accacaatat tggaagctta ttagcctttc     6600

tattttttcg tttgcaaggc ttaacaacat tgctgtggag agggatgggg atatggaggc     6660

cgctggaggg agtcggagag gcgttttgga gcggcttggc ctggcgccca gctcgcgaaa     6720

cgcacctagg accctttggc acgccgaaat gtgccacttt tcagtctagt aacgccttac     6780

ctacgtcatt ccatgcgtgc atgtttgcgc cttttttccc ttgcccttga tcgccacaca     6840

gtacagtgca ctgtacagtg gaggttttgg gggggtctta gatgggagct aaaagcggcc     6900

tagcggtaca ctagtgggat tgtatggagt ggcatggagc ctaggtggag cctgacagga     6960

cgcacgaccg gctagcccgt gacagacgat gggtggctcc tgttgtccac cgcgtacaaa     7020

tgtttgggcc aaagtcttgt cagccttgct tgcgaaccta attcccaatt ttgtcacttc     7080

gcacccccat tgatcgagcc ctaacccctg cccatcaggc aatccaatta agctcgcatt     7140

gtctgccttg tttagtttgg ctcctgcccg tttcggcgtc cacttgcaca aacacaaaca     7200

agcattatat ataaggctcg tctctccctc ccaaccacac tcactttttt gcccgtcttc     7260

ccttgctaac acaaaagtca agaacacaaa caaccacccc aaccccctta cacacaagac     7320

atatctacag caatggccat ggccaaaagc aaacgacggt cggaggctgt ggaagagcac     7380

gtgaccggct cggacgaggg cttgaccgat acttcgggtc acgtgagccc tgccgccaag     7440

aagcagaaga actcggagat tcatttcacc acccaggctg cccagcagtt ggatcgggag     7500

cgcaaggagg agtatctgga ctcgctgatc gacaacaagg actatctcaa gtaccgtcct     7560

cgaggctgga agctcaacaa cccgcctacc gaccgacctg tgcgaatcta cgccgatgga     7620

gtgtttgatt tgttccatct gggacacatg cgtcagctgg agcagtccaa gaaggccttc     7680

cccaacgcag tgttgattgt gggcattccc agcgacaagg agacccacaa gcggaaggga     7740

ttgaccgtgc tgagtgacgt ccagcggtac gagacggtgc gacactgcaa gtgggtggac     7800

gaggtggtgg aggatgctcc ctggtgtgtc accatggact ttctggaaaa acacaaaatc     7860

gactacgtgg cccatgacga tctgccctac gcttccggca acgacgatga tatctacaag     7920

cccatcaagg agaagggcat gtttctggcc acccagcgaa ccgagggcat ttccacctcg     7980

gacatcatca ccaagattat ccgagactac gacaagtatt taatgcgaaa ctttgcccgg     8040

ggtgctaacc gaaaggatct caacgtctcg tggctcaaga agaacgagct ggacttcaag     8100

cgtcatgtgg ccgagttccg aaactcgttc aagcgaaaga aggtcggtaa ggatctctac     8160

ggcgagattc gcggtctgct gcagaatgtg ctcatttgga acggcgacaa ctccggcact     8220

tccactcccc agcgaaagac gctgcagacc aacgccaaga agatgtacat gaacgtgctc     8280

aagactctgc aggctcctga cgctgttgac gtggactcct cggagaacgt gtctgagaac     8340

gtcactgatg aggaggagga agacgacgac gaggttgatg aggacgaaga agccgacgac     8400

gacgacgaag acgacgaaga cgaggaagac gacgagtagg cggccgcatt gatgattgga     8460

aacacacaca tgggttatat ctaggtgaga gttagttgga cagttatata ttaaatcagc     8520

tatgccaacg gtaacttcat tcatgtcaac gaggaaccag tgactgcaag taatatagaa     8580

tttgaccacc ttgccattct cttgcactcc tttactatat ctcatttatt tcttatatac     8640

aaatcacttc ttcttcccag catcgagctc ggaaacctca tgagcaataa catcgtggat     8700

ctcgtcaata gagggctttt tggactcctt gctgttggcc accttgtcct tgctgtttaa     8760

acacgcagta ggatgtcctg cacgggtctt tttgtggggt gtggagaaag gggtgcttgg     8820

agatggaagc cggtagaacc gggctgcttg tgcttggaga tggaagccgg tagaaccggg     8880

ctgcttgggg ggatttgggg ccgctgggct ccaaagaggg gtaggcattt cgttggggtt     8940

acgtaattgc ggcatttggg tcctgcgcgc atgtcccatt ggtcagaatt agtccggata     9000

ggagacttat cagccaatca cagcgccgga tccacctgta ggttgggttg ggtgggagca     9060

cccctccaca gagtagagtc aaacagcagc agcaacatga tagttggggg tgtgcgtgtt     9120

aaaggaaaaa aaagaagctt gggttatatt cccgctctat ttagaggttg cgggatagac     9180

gccgacggag ggcaatggcg ctatggaacc ttgcggatat ccatacgccg cggcggactg     9240

cgtccgaacc agctccagca gcgttttttc cgggccattg agccgactgc gaccccgcca     9300

acgtgtcttg gcccacgcac tcatgtcatg ttggtgttgg gaggccactt tttaagtagc     9360

acaaggcacc tagctcgcag caaggtgtcc gaaccaaaga agcggctgca gtggtgcaaa     9420

cggggcggaa acggcgggaa aaagccacgg gggcacgaat tgaggcacgc cctcgaattt     9480

gagacgagtc acggccccat tcgcccgcgc aatggctcgc caacgcccgg tcttttgcac     9540

cacatcaggt taccccaagc caaacctttg tgttaaaaag cttaacatat tataccgaac     9600

gtaggtttgg gcgggcttgc tccgtctgtc caaggcaaca tttatataag ggtctgcatc     9660

gccggctcaa ttgaatcttt tttcttcttc tcttctctat attcattctt gaattaaaca     9720

cacatcaaca tggccatcaa agtcggtatt aacggattcg ggcgaatcgg acgaattgtg     9780

agtaccatag aaggtgatgg aaacatgacc caacagaaac agatgacaag tgtcatcgac     9840

ccaccagagc ccaattgagc tcatactaac agtcgacaac ctgtcgaacc aattgatgac     9900

tccccgacaa tgtactaaca caggtcctgc ccatggtgaa aaacgtggac caagtggatc     9960

tctcgcaggt cgacaccatt gcctccggcc gagatgtcaa ctacaaggtc aagtacacct    10020

ccggcgttaa gatgagccag ggcgcctacg acgacaaggg ccgccacatt tccgagcagc    10080

ccttcacctg ggccaactgg caccagcaca tcaactggct caacttcatt ctggtgattg    10140

cgctgcctct gtcgtccttt gctgccgctc ccttcgtctc cttcaactgg aagaccgccg    10200

cgtttgctgt cggctattac atgtgcaccg gtctcggtat caccgccggc taccaccgaa    10260

tgtgggccca tcgagcctac aaggccgctc tgcccgttcg aatcatcctt gctctgtttg    10320

gaggaggagc tgtcgagggc tccatccgat ggtgggcctc gtctcaccga gtccaccacc    10380

gatggaccga ctccaacaag gacccttacg acgcccgaaa gggattctgg ttctcccact    10440

ttggctggat gctgcttgtg cccaacccca agaacaaggg ccgaactgac atttctgacc    10500

tcaacaacga ctgggttgtc cgactccagc acaagtacta cgtttacgtt ctcgtcttca    10560

tggccattgt tctgcccacc ctcgtctgtg gctttggctg gggcgactgg aagggaggtc    10620

ttgtctacgc cggtatcatg cgatacacct ttgtgcagca ggtgactttc tgtgtcaact    10680

cccttgccca ctggattgga gagcagccct tcgacgaccg acgaactccc cgagaccacg    10740

ctcttaccgc cctggtcacc tttggagagg gctaccacaa cttccaccac gagttcccct    10800

cggactaccg aaacgccctc atctggtacc agtacgaccc caccaagtgg ctcatctgga    10860

ccctcaagca ggttggtctc gcctgggacc tccagacctt ctcccagaac gccatcgagc    10920

agggtctcgt gcagcagcga cagaagaagc tggacaagtg gcgaaacaac ctcaactggg    10980

gtatccccat tgagcagctg cctgtcattg agtttgagga gttccaagag caggccaaga    11040

cccgagatct ggttctcatt tctggcattg tccacgacgt gtctgccttt gtcgagcacc    11100

accctggtgg aaaggccctc attatgagcg ccgtcggcaa ggacggtacc gctgtcttca    11160

acggaggtgt ctaccgacac tccaacgctg gccacaacct gcttgccacc atgcgagttt    11220

cggtcattcg aggcggcatg gaggttgagg tgtggaagac tgcccagaac gaaaagaagg    11280

accagaacat tgtctccgat gagagtggaa accgaatcca ccgagctggt ctccaggcca    11340

cccgggtcga gaaccccggt atgtctggca tggctgctta ggcggccgca tgagaagata    11400

aatatataaa tacattgaga tattaaatgc gctagattag agagcctcat actgctcgga    11460

gagaagccaa gacgagtact caaaggggat tacaccatcc atatccacag acacaagctg    11520

gggaaaggtt ctatatacac tttccggaat accgtagttt ccgatgttat caatgggggc    11580

agccaggatt tcaggcactt cggtgtctcg gggtgaaatg gcgttcttgg cctccatcaa    11640

gtcgtaccat gtcttcattt gcctgtcaaa gtaaaacaga agcagatgaa gaatgaactt    11700

gaagtgaagg aatttaaata gttggagcaa gggagaaatg tagagtgtga aagactcact    11760

atggtccggg cttatctcga ccaatagcca aagtctggag tttctgagag aaaaaggcaa    11820

gatacgtatg taacaaagcg acgcatggta caataatacc ggaggcatgt atcatagaga    11880

gttagtggtt cgatgatggc actggtgcct ggtatgactt tatacggctg actacatatt    11940

tgtcctcaga catacaatta cagtcaagca cttacccttg gacatctgta ggtacccccc    12000

ggccaagacg atctcagcgt gtcgtatgtc ggattggcgt agctccctcg ctcgtcaatt    12060

ggctcccatc tactttcttc tgcttggcta cacccagcat gtctgctatg gctcgttttc    12120

gtgccttatc tatcctccca gtattaccaa ctctaaatga catgatgtga ttgggtctac    12180

actttcatat cagagataag gagtagcaca gttgcataaa aagcccaact ctaatcagct    12240

tcttcctttc ttgtaattag tacaaaggtg attagcgaaa tctggaagct tagttggccc    12300

taaaaaaatc aaaaaaagca aaaaacgaaa aacgaaaaac cacagttttg agaacaggga    12360

ggtaacgaag gatcgtatat atatatatat atatatatac ccacggatcc cgagaccggc    12420

ctttgattct tccctacaac caaccattct caccacccta attcacaacc atggaggtcg    12480

tgaacgaaat cgtctccatt ggccaggagg ttcttcccaa ggtcgactat gctcagctct    12540

ggtctgatgc ctcgcactgc gaggtgctgt acctctccat cgccttcgtc atcctgaagt    12600

tcacccttgg tcctctcgga cccaagggtc agtctcgaat gaagtttgtg ttcaccaact    12660

acaacctgct catgtccatc tactcgctgg gctccttcct ctctatggcc tacgccatgt    12720

acaccattgg tgtcatgtcc gacaactgcg agaaggcttt cgacaacaat gtcttccgaa    12780

tcaccactca gctgttctac ctcagcaagt tcctcgagta cattgactcc ttctatctgc    12840

ccctcatggg caagcctctg acctggttgc agttctttca ccatctcgga gctcctatgg    12900

acatgtggct gttctacaac taccgaaacg aagccgtttg gatctttgtg ctgctcaacg    12960

gcttcattca ctggatcatg tacggctact attggacccg actgatcaag ctcaagttcc    13020

ctatgcccaa gtccctgatt acttctatgc agatcattca gttcaacgtt ggcttctaca    13080

tcgtctggaa gtaccggaac attccctgct accgacaaga tggaatgaga atgtttggct    13140

ggtttttcaa ctacttctac gttggtactg tcctgtgtct gttcctcaac ttctacgtgc    13200

agacctacat cgtccgaaag cacaagggag ccaaaaagat tcagtgagcg gccgcaagtg    13260

tggatgggga agtgagtgcc cggttctgtg tgcacaattg gcaatccaag atggatggat    13320

tcaacacagg gatatagcga gctacgtggt ggtgcgagga tatagcaacg gatatttatg    13380

tttgacactt gagaatgtac gatacaagca ctgtccaagt acaatactaa acatactgta    13440

catactcata ctcgtacccg gcaacggttt cacttgagtg cagtggctag tgctcttact    13500

cgtacagtgt gcaatactgc gtatcatagt ctttgatgta tatcgtattc attcatgtta    13560

gttgc                                                                13565


<210>  3
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35G"

<400>  3
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ggg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  4
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  4

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  5
<211>  1449
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1449)
<223>  delta-9 desaturase; GenBank Accession No. XM_501496

<400>  5
atg gtg aaa aac gtg gac caa gtg gat ctc tcg cag gtc gac acc att         48
Met Val Lys Asn Val Asp Gln Val Asp Leu Ser Gln Val Asp Thr Ile           
1               5                   10                  15                

gcc tcc ggc cga gat gtc aac tac aag gtc aag tac acc tcc ggc gtt         96
Ala Ser Gly Arg Asp Val Asn Tyr Lys Val Lys Tyr Thr Ser Gly Val           
            20                  25                  30                    

aag atg agc cag ggc gcc tac gac gac aag ggc cgc cac att tcc gag        144
Lys Met Ser Gln Gly Ala Tyr Asp Asp Lys Gly Arg His Ile Ser Glu           
        35                  40                  45                        

cag ccc ttc acc tgg gcc aac tgg cac cag cac atc aac tgg ctc aac        192
Gln Pro Phe Thr Trp Ala Asn Trp His Gln His Ile Asn Trp Leu Asn           
    50                  55                  60                            

ttc att ctg gtg att gcg ctg cct ctg tcg tcc ttt gct gcc gct ccc        240
Phe Ile Leu Val Ile Ala Leu Pro Leu Ser Ser Phe Ala Ala Ala Pro           
65                  70                  75                  80            

ttc gtc tcc ttc aac tgg aag acc gcc gcg ttt gct gtc ggc tat tac        288
Phe Val Ser Phe Asn Trp Lys Thr Ala Ala Phe Ala Val Gly Tyr Tyr           
                85                  90                  95                

atg tgc acc ggt ctc ggt atc acc gcc ggc tac cac cga atg tgg gcc        336
Met Cys Thr Gly Leu Gly Ile Thr Ala Gly Tyr His Arg Met Trp Ala           
            100                 105                 110                   

cat cga gcc tac aag gcc gct ctg ccc gtt cga atc atc ctt gct ctg        384
His Arg Ala Tyr Lys Ala Ala Leu Pro Val Arg Ile Ile Leu Ala Leu           
        115                 120                 125                       

ttt gga gga gga gct gtc gag ggc tcc atc cga tgg tgg gcc tcg tct        432
Phe Gly Gly Gly Ala Val Glu Gly Ser Ile Arg Trp Trp Ala Ser Ser           
    130                 135                 140                           

cac cga gtc cac cac cga tgg acc gac tcc aac aag gac cct tac gac        480
His Arg Val His His Arg Trp Thr Asp Ser Asn Lys Asp Pro Tyr Asp           
145                 150                 155                 160           

gcc cga aag gga ttc tgg ttc tcc cac ttt ggc tgg atg ctg ctt gtg        528
Ala Arg Lys Gly Phe Trp Phe Ser His Phe Gly Trp Met Leu Leu Val           
                165                 170                 175               

ccc aac ccc aag aac aag ggc cga act gac att tct gac ctc aac aac        576
Pro Asn Pro Lys Asn Lys Gly Arg Thr Asp Ile Ser Asp Leu Asn Asn           
            180                 185                 190                   

gac tgg gtt gtc cga ctc cag cac aag tac tac gtt tac gtt ctc gtc        624
Asp Trp Val Val Arg Leu Gln His Lys Tyr Tyr Val Tyr Val Leu Val           
        195                 200                 205                       

ttc atg gcc att gtt ctg ccc acc ctc gtc tgt ggc ttt ggc tgg ggc        672
Phe Met Ala Ile Val Leu Pro Thr Leu Val Cys Gly Phe Gly Trp Gly           
    210                 215                 220                           

gac tgg aag gga ggt ctt gtc tac gcc ggt atc atg cga tac acc ttt        720
Asp Trp Lys Gly Gly Leu Val Tyr Ala Gly Ile Met Arg Tyr Thr Phe           
225                 230                 235                 240           

gtg cag cag gtg act ttc tgt gtc aac tcc ctt gcc cac tgg att gga        768
Val Gln Gln Val Thr Phe Cys Val Asn Ser Leu Ala His Trp Ile Gly           
                245                 250                 255               

gag cag ccc ttc gac gac cga cga act ccc cga gac cac gct ctt acc        816
Glu Gln Pro Phe Asp Asp Arg Arg Thr Pro Arg Asp His Ala Leu Thr           
            260                 265                 270                   

gcc ctg gtc acc ttt gga gag ggc tac cac aac ttc cac cac gag ttc        864
Ala Leu Val Thr Phe Gly Glu Gly Tyr His Asn Phe His His Glu Phe           
        275                 280                 285                       

ccc tcg gac tac cga aac gcc ctc atc tgg tac cag tac gac ccc acc        912
Pro Ser Asp Tyr Arg Asn Ala Leu Ile Trp Tyr Gln Tyr Asp Pro Thr           
    290                 295                 300                           

aag tgg ctc atc tgg acc ctc aag cag gtt ggt ctc gcc tgg gac ctc        960
Lys Trp Leu Ile Trp Thr Leu Lys Gln Val Gly Leu Ala Trp Asp Leu           
305                 310                 315                 320           

cag acc ttc tcc cag aac gcc atc gag cag ggt ctc gtg cag cag cga       1008
Gln Thr Phe Ser Gln Asn Ala Ile Glu Gln Gly Leu Val Gln Gln Arg           
                325                 330                 335               

cag aag aag ctg gac aag tgg cga aac aac ctc aac tgg ggt atc ccc       1056
Gln Lys Lys Leu Asp Lys Trp Arg Asn Asn Leu Asn Trp Gly Ile Pro           
            340                 345                 350                   

att gag cag ctg cct gtc att gag ttt gag gag ttc caa gag cag gcc       1104
Ile Glu Gln Leu Pro Val Ile Glu Phe Glu Glu Phe Gln Glu Gln Ala           
        355                 360                 365                       

aag acc cga gat ctg gtt ctc att tct ggc att gtc cac gac gtg tct       1152
Lys Thr Arg Asp Leu Val Leu Ile Ser Gly Ile Val His Asp Val Ser           
    370                 375                 380                           

gcc ttt gtc gag cac cac cct ggt gga aag gcc ctc att atg agc gcc       1200
Ala Phe Val Glu His His Pro Gly Gly Lys Ala Leu Ile Met Ser Ala           
385                 390                 395                 400           

gtc ggc aag gac ggt acc gct gtc ttc aac gga ggt gtc tac cga cac       1248
Val Gly Lys Asp Gly Thr Ala Val Phe Asn Gly Gly Val Tyr Arg His           
                405                 410                 415               

tcc aac gct ggc cac aac ctg ctt gcc acc atg cga gtt tcg gtc att       1296
Ser Asn Ala Gly His Asn Leu Leu Ala Thr Met Arg Val Ser Val Ile           
            420                 425                 430                   

cga ggc ggc atg gag gtt gag gtg tgg aag act gcc cag aac gaa aag       1344
Arg Gly Gly Met Glu Val Glu Val Trp Lys Thr Ala Gln Asn Glu Lys           
        435                 440                 445                       

aag gac cag aac att gtc tcc gat gag agt gga aac cga atc cac cga       1392
Lys Asp Gln Asn Ile Val Ser Asp Glu Ser Gly Asn Arg Ile His Arg           
    450                 455                 460                           

gct ggt ctc cag gcc acc cgg gtc gag aac ccc ggt atg tct ggc atg       1440
Ala Gly Leu Gln Ala Thr Arg Val Glu Asn Pro Gly Met Ser Gly Met           
465                 470                 475                 480           

gct gct tag                                                           1449
Ala Ala                                                                   
                                                                          


<210>  6
<211>  482
<212>  PRT
<213>  Yarrowia lipolytica

<400>  6

Met Val Lys Asn Val Asp Gln Val Asp Leu Ser Gln Val Asp Thr Ile 
1               5                   10                  15      


Ala Ser Gly Arg Asp Val Asn Tyr Lys Val Lys Tyr Thr Ser Gly Val 
            20                  25                  30          


Lys Met Ser Gln Gly Ala Tyr Asp Asp Lys Gly Arg His Ile Ser Glu 
        35                  40                  45              


Gln Pro Phe Thr Trp Ala Asn Trp His Gln His Ile Asn Trp Leu Asn 
    50                  55                  60                  


Phe Ile Leu Val Ile Ala Leu Pro Leu Ser Ser Phe Ala Ala Ala Pro 
65                  70                  75                  80  


Phe Val Ser Phe Asn Trp Lys Thr Ala Ala Phe Ala Val Gly Tyr Tyr 
                85                  90                  95      


Met Cys Thr Gly Leu Gly Ile Thr Ala Gly Tyr His Arg Met Trp Ala 
            100                 105                 110         


His Arg Ala Tyr Lys Ala Ala Leu Pro Val Arg Ile Ile Leu Ala Leu 
        115                 120                 125             


Phe Gly Gly Gly Ala Val Glu Gly Ser Ile Arg Trp Trp Ala Ser Ser 
    130                 135                 140                 


His Arg Val His His Arg Trp Thr Asp Ser Asn Lys Asp Pro Tyr Asp 
145                 150                 155                 160 


Ala Arg Lys Gly Phe Trp Phe Ser His Phe Gly Trp Met Leu Leu Val 
                165                 170                 175     


Pro Asn Pro Lys Asn Lys Gly Arg Thr Asp Ile Ser Asp Leu Asn Asn 
            180                 185                 190         


Asp Trp Val Val Arg Leu Gln His Lys Tyr Tyr Val Tyr Val Leu Val 
        195                 200                 205             


Phe Met Ala Ile Val Leu Pro Thr Leu Val Cys Gly Phe Gly Trp Gly 
    210                 215                 220                 


Asp Trp Lys Gly Gly Leu Val Tyr Ala Gly Ile Met Arg Tyr Thr Phe 
225                 230                 235                 240 


Val Gln Gln Val Thr Phe Cys Val Asn Ser Leu Ala His Trp Ile Gly 
                245                 250                 255     


Glu Gln Pro Phe Asp Asp Arg Arg Thr Pro Arg Asp His Ala Leu Thr 
            260                 265                 270         


Ala Leu Val Thr Phe Gly Glu Gly Tyr His Asn Phe His His Glu Phe 
        275                 280                 285             


Pro Ser Asp Tyr Arg Asn Ala Leu Ile Trp Tyr Gln Tyr Asp Pro Thr 
    290                 295                 300                 


Lys Trp Leu Ile Trp Thr Leu Lys Gln Val Gly Leu Ala Trp Asp Leu 
305                 310                 315                 320 


Gln Thr Phe Ser Gln Asn Ala Ile Glu Gln Gly Leu Val Gln Gln Arg 
                325                 330                 335     


Gln Lys Lys Leu Asp Lys Trp Arg Asn Asn Leu Asn Trp Gly Ile Pro 
            340                 345                 350         


Ile Glu Gln Leu Pro Val Ile Glu Phe Glu Glu Phe Gln Glu Gln Ala 
        355                 360                 365             


Lys Thr Arg Asp Leu Val Leu Ile Ser Gly Ile Val His Asp Val Ser 
    370                 375                 380                 


Ala Phe Val Glu His His Pro Gly Gly Lys Ala Leu Ile Met Ser Ala 
385                 390                 395                 400 


Val Gly Lys Asp Gly Thr Ala Val Phe Asn Gly Gly Val Tyr Arg His 
                405                 410                 415     


Ser Asn Ala Gly His Asn Leu Leu Ala Thr Met Arg Val Ser Val Ile 
            420                 425                 430         


Arg Gly Gly Met Glu Val Glu Val Trp Lys Thr Ala Gln Asn Glu Lys 
        435                 440                 445             


Lys Asp Gln Asn Ile Val Ser Asp Glu Ser Gly Asn Arg Ile His Arg 
    450                 455                 460                 


Ala Gly Leu Gln Ala Thr Arg Val Glu Asn Pro Gly Met Ser Gly Met 
465                 470                 475                 480 


Ala Ala 
        


<210>  7
<211>  1101
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1101)
<223>  choline-phosphate cytidylyl-transferase; GenBank Accession No. 
       XM_502978

<400>  7
atg gcc aaa agc aaa cga cgg tcg gag gct gtg gaa gag cac gtg acc         48
Met Ala Lys Ser Lys Arg Arg Ser Glu Ala Val Glu Glu His Val Thr           
1               5                   10                  15                

ggc tcg gac gag ggc ttg acc gat act tcg ggt cac gtg agc cct gcc         96
Gly Ser Asp Glu Gly Leu Thr Asp Thr Ser Gly His Val Ser Pro Ala           
            20                  25                  30                    

gcc aag aag cag aag aac tcg gag att cat ttc acc acc cag gct gcc        144
Ala Lys Lys Gln Lys Asn Ser Glu Ile His Phe Thr Thr Gln Ala Ala           
        35                  40                  45                        

cag cag ttg gat cgg gag cgc aag gag gag tat ctg gac tcg ctg atc        192
Gln Gln Leu Asp Arg Glu Arg Lys Glu Glu Tyr Leu Asp Ser Leu Ile           
    50                  55                  60                            

gac aac aag gac tat ctc aag tac cgt cct cga ggc tgg aag ctc aac        240
Asp Asn Lys Asp Tyr Leu Lys Tyr Arg Pro Arg Gly Trp Lys Leu Asn           
65                  70                  75                  80            

aac ccg cct acc gac cga cct gtg cga atc tac gcc gat gga gtg ttt        288
Asn Pro Pro Thr Asp Arg Pro Val Arg Ile Tyr Ala Asp Gly Val Phe           
                85                  90                  95                

gat ttg ttc cat ctg gga cac atg cgt cag ctg gag cag tcc aag aag        336
Asp Leu Phe His Leu Gly His Met Arg Gln Leu Glu Gln Ser Lys Lys           
            100                 105                 110                   

gcc ttc ccc aac gca gtg ttg att gtg ggc att ccc agc gac aag gag        384
Ala Phe Pro Asn Ala Val Leu Ile Val Gly Ile Pro Ser Asp Lys Glu           
        115                 120                 125                       

acc cac aag cgg aag gga ttg acc gtg ctg agt gac gtc cag cgg tac        432
Thr His Lys Arg Lys Gly Leu Thr Val Leu Ser Asp Val Gln Arg Tyr           
    130                 135                 140                           

gag acg gtg cga cac tgc aag tgg gtg gac gag gtg gtg gag gat gct        480
Glu Thr Val Arg His Cys Lys Trp Val Asp Glu Val Val Glu Asp Ala           
145                 150                 155                 160           

ccc tgg tgt gtc acc atg gac ttt ctg gaa aaa cac aaa atc gac tac        528
Pro Trp Cys Val Thr Met Asp Phe Leu Glu Lys His Lys Ile Asp Tyr           
                165                 170                 175               

gtg gcc cat gac gat ctg ccc tac gct tcc ggc aac gac gat gat atc        576
Val Ala His Asp Asp Leu Pro Tyr Ala Ser Gly Asn Asp Asp Asp Ile           
            180                 185                 190                   

tac aag ccc atc aag gag aag ggc atg ttt ctg gcc acc cag cga acc        624
Tyr Lys Pro Ile Lys Glu Lys Gly Met Phe Leu Ala Thr Gln Arg Thr           
        195                 200                 205                       

gag ggc att tcc acc tcg gac atc atc acc aag att atc cga gac tac        672
Glu Gly Ile Ser Thr Ser Asp Ile Ile Thr Lys Ile Ile Arg Asp Tyr           
    210                 215                 220                           

gac aag tat tta atg cga aac ttt gcc cgg ggt gct aac cga aag gat        720
Asp Lys Tyr Leu Met Arg Asn Phe Ala Arg Gly Ala Asn Arg Lys Asp           
225                 230                 235                 240           

ctc aac gtc tcg tgg ctc aag aag aac gag ctg gac ttc aag cgt cat        768
Leu Asn Val Ser Trp Leu Lys Lys Asn Glu Leu Asp Phe Lys Arg His           
                245                 250                 255               

gtg gcc gag ttc cga aac tcg ttc aag cga aag aag gtc ggt aag gat        816
Val Ala Glu Phe Arg Asn Ser Phe Lys Arg Lys Lys Val Gly Lys Asp           
            260                 265                 270                   

ctc tac ggc gag att cgc ggt ctg ctg cag aat gtg ctc att tgg aac        864
Leu Tyr Gly Glu Ile Arg Gly Leu Leu Gln Asn Val Leu Ile Trp Asn           
        275                 280                 285                       

ggc gac aac tcc ggc act tcc act ccc cag cga aag acg ctg cag acc        912
Gly Asp Asn Ser Gly Thr Ser Thr Pro Gln Arg Lys Thr Leu Gln Thr           
    290                 295                 300                           

aac gcc aag aag atg tac atg aac gtg ctc aag act ctg cag gct cct        960
Asn Ala Lys Lys Met Tyr Met Asn Val Leu Lys Thr Leu Gln Ala Pro           
305                 310                 315                 320           

gac gct gtt gac gtg gac tcc tcg gag aac gtg tct gag aac gtc act       1008
Asp Ala Val Asp Val Asp Ser Ser Glu Asn Val Ser Glu Asn Val Thr           
                325                 330                 335               

gat gag gag gag gaa gac gac gac gag gtt gat gag gac gaa gaa gcc       1056
Asp Glu Glu Glu Glu Asp Asp Asp Glu Val Asp Glu Asp Glu Glu Ala           
            340                 345                 350                   

gac gac gac gac gaa gac gac gaa gac gag gaa gac gac gag tag           1101
Asp Asp Asp Asp Glu Asp Asp Glu Asp Glu Glu Asp Asp Glu                   
        355                 360                 365                       


<210>  8
<211>  366
<212>  PRT
<213>  Yarrowia lipolytica

<400>  8

Met Ala Lys Ser Lys Arg Arg Ser Glu Ala Val Glu Glu His Val Thr 
1               5                   10                  15      


Gly Ser Asp Glu Gly Leu Thr Asp Thr Ser Gly His Val Ser Pro Ala 
            20                  25                  30          


Ala Lys Lys Gln Lys Asn Ser Glu Ile His Phe Thr Thr Gln Ala Ala 
        35                  40                  45              


Gln Gln Leu Asp Arg Glu Arg Lys Glu Glu Tyr Leu Asp Ser Leu Ile 
    50                  55                  60                  


Asp Asn Lys Asp Tyr Leu Lys Tyr Arg Pro Arg Gly Trp Lys Leu Asn 
65                  70                  75                  80  


Asn Pro Pro Thr Asp Arg Pro Val Arg Ile Tyr Ala Asp Gly Val Phe 
                85                  90                  95      


Asp Leu Phe His Leu Gly His Met Arg Gln Leu Glu Gln Ser Lys Lys 
            100                 105                 110         


Ala Phe Pro Asn Ala Val Leu Ile Val Gly Ile Pro Ser Asp Lys Glu 
        115                 120                 125             


Thr His Lys Arg Lys Gly Leu Thr Val Leu Ser Asp Val Gln Arg Tyr 
    130                 135                 140                 


Glu Thr Val Arg His Cys Lys Trp Val Asp Glu Val Val Glu Asp Ala 
145                 150                 155                 160 


Pro Trp Cys Val Thr Met Asp Phe Leu Glu Lys His Lys Ile Asp Tyr 
                165                 170                 175     


Val Ala His Asp Asp Leu Pro Tyr Ala Ser Gly Asn Asp Asp Asp Ile 
            180                 185                 190         


Tyr Lys Pro Ile Lys Glu Lys Gly Met Phe Leu Ala Thr Gln Arg Thr 
        195                 200                 205             


Glu Gly Ile Ser Thr Ser Asp Ile Ile Thr Lys Ile Ile Arg Asp Tyr 
    210                 215                 220                 


Asp Lys Tyr Leu Met Arg Asn Phe Ala Arg Gly Ala Asn Arg Lys Asp 
225                 230                 235                 240 


Leu Asn Val Ser Trp Leu Lys Lys Asn Glu Leu Asp Phe Lys Arg His 
                245                 250                 255     


Val Ala Glu Phe Arg Asn Ser Phe Lys Arg Lys Lys Val Gly Lys Asp 
            260                 265                 270         


Leu Tyr Gly Glu Ile Arg Gly Leu Leu Gln Asn Val Leu Ile Trp Asn 
        275                 280                 285             


Gly Asp Asn Ser Gly Thr Ser Thr Pro Gln Arg Lys Thr Leu Gln Thr 
    290                 295                 300                 


Asn Ala Lys Lys Met Tyr Met Asn Val Leu Lys Thr Leu Gln Ala Pro 
305                 310                 315                 320 


Asp Ala Val Asp Val Asp Ser Ser Glu Asn Val Ser Glu Asn Val Thr 
                325                 330                 335     


Asp Glu Glu Glu Glu Asp Asp Asp Glu Val Asp Glu Asp Glu Glu Ala 
            340                 345                 350         


Asp Asp Asp Asp Glu Asp Asp Glu Asp Glu Glu Asp Asp Glu 
        355                 360                 365     


