                         SEQUENCE LISTING

<110>  Baystate Medical Center, Inc.
       University of Massachusetts
       Yadava, Nagendra
       Kim, Chul
       Heuck, Alejandro P
 
<120>  METHODS, COMPOSITIONS AND KITS FOR ASSAYING MITOCHONDRIAL 
       FUNCTION

<130>  P0963.70000

<150>  61/473,730
<151>  2011-04-08

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  500
<212>  PRT
<213>  Clostridium perfringens

<400>  1

Met Ile Arg Phe Lys Lys Thr Lys Leu Ile Ala Ser Ile Ala Met Ala 
1               5                   10                  15      


Leu Cys Leu Phe Ser Gln Pro Val Ile Ser Phe Ser Lys Asp Ile Thr 
            20                  25                  30          


Asp Lys Asn Gln Ser Ile Asp Ser Gly Ile Ser Ser Leu Ser Tyr Asn 
        35                  40                  45              


Arg Asn Glu Val Leu Ala Ser Asn Gly Asp Lys Ile Glu Ser Phe Val 
    50                  55                  60                  


Pro Lys Glu Gly Lys Lys Thr Gly Asn Lys Phe Ile Val Val Glu Arg 
65                  70                  75                  80  


Gln Lys Arg Ser Leu Thr Thr Ser Pro Val Asp Ile Ser Ile Ile Asp 
                85                  90                  95      


Ser Val Asn Asp Arg Thr Tyr Pro Gly Ala Leu Gln Leu Ala Asp Lys 
            100                 105                 110         


Ala Phe Val Glu Asn Arg Pro Thr Ile Leu Met Val Lys Arg Lys Pro 
        115                 120                 125             


Ile Asn Ile Asn Ile Asp Leu Pro Gly Leu Lys Gly Glu Asn Ser Ile 
    130                 135                 140                 


Lys Val Asp Asp Pro Thr Tyr Gly Lys Val Ser Gly Ala Ile Asp Glu 
145                 150                 155                 160 


Leu Val Ser Lys Trp Asn Glu Lys Tyr Ser Ser Thr His Thr Leu Pro 
                165                 170                 175     


Ala Arg Thr Gln Tyr Ser Glu Ser Met Val Tyr Ser Lys Ser Gln Ile 
            180                 185                 190         


Ser Ser Ala Leu Asn Val Asn Ala Lys Val Leu Glu Asn Ser Leu Gly 
        195                 200                 205             


Val Asp Phe Asn Ala Val Ala Asn Asn Glu Lys Lys Val Met Ile Leu 
    210                 215                 220                 


Ala Tyr Lys Gln Ile Phe Tyr Thr Val Ser Ala Asp Leu Pro Lys Asn 
225                 230                 235                 240 


Pro Ser Asp Leu Phe Asp Asp Ser Val Thr Phe Asn Asp Leu Lys Gln 
                245                 250                 255     


Lys Gly Val Ser Asn Glu Ala Pro Pro Leu Met Val Ser Asn Val Ala 
            260                 265                 270         


Tyr Gly Arg Thr Ile Tyr Val Lys Leu Glu Thr Thr Ser Ser Ser Lys 
        275                 280                 285             


Asp Val Gln Ala Ala Phe Lys Ala Leu Ile Lys Asn Thr Asp Ile Lys 
    290                 295                 300                 


Asn Ser Gln Gln Tyr Lys Asp Ile Tyr Glu Asn Ser Ser Phe Thr Ala 
305                 310                 315                 320 


Val Val Leu Gly Gly Asp Ala Gln Glu His Asn Lys Val Val Thr Lys 
                325                 330                 335     


Asp Phe Asp Glu Ile Arg Lys Val Ile Lys Asp Asn Ala Thr Phe Ser 
            340                 345                 350         


Thr Lys Asn Pro Ala Tyr Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys 
        355                 360                 365             


Asp Asn Ser Val Ala Ala Val His Asn Lys Thr Asp Tyr Ile Glu Thr 
    370                 375                 380                 


Thr Ser Thr Glu Tyr Ser Lys Gly Lys Ile Asn Leu Asp His Ser Gly 
385                 390                 395                 400 


Ala Tyr Val Ala Gln Phe Glu Val Ala Trp Asp Glu Val Ser Tyr Asp 
                405                 410                 415     


Lys Glu Gly Asn Glu Val Leu Thr His Lys Thr Trp Asp Gly Asn Tyr 
            420                 425                 430         


Gln Asp Lys Thr Ala His Tyr Ser Thr Val Ile Pro Leu Glu Ala Asn 
        435                 440                 445             


Ala Arg Asn Ile Arg Ile Lys Ala Arg Glu Cys Thr Gly Leu Ala Trp 
    450                 455                 460                 


Glu Trp Trp Arg Asp Val Ile Ser Glu Tyr Asp Val Pro Leu Thr Asn 
465                 470                 475                 480 


Asn Ile Asn Val Ser Ile Trp Gly Thr Thr Leu Tyr Pro Gly Ser Ser 
                485                 490                 495     


Ile Thr Tyr Asn 
            500 


<210>  2
<211>  500
<212>  PRT
<213>  Clostridium perfringens

<400>  2

Met Ile Arg Phe Lys Lys Thr Lys Leu Ile Ala Ser Ile Ala Met Ala 
1               5                   10                  15      


Leu Cys Leu Phe Ser Gln Pro Val Ile Ser Phe Ser Lys Asp Ile Thr 
            20                  25                  30          


Asp Lys Asn Gln Ser Ile Asp Ser Gly Ile Ser Ser Leu Ser Tyr Asn 
        35                  40                  45              


Arg Asn Glu Val Leu Ala Ser Asn Gly Asp Lys Ile Glu Ser Phe Val 
    50                  55                  60                  


Pro Lys Glu Gly Lys Lys Ala Gly Asn Lys Phe Ile Val Val Glu Arg 
65                  70                  75                  80  


Gln Lys Arg Ser Leu Thr Thr Ser Pro Val Asp Ile Ser Ile Ile Asp 
                85                  90                  95      


Ser Val Asn Asp Arg Thr Tyr Pro Gly Ala Leu Gln Leu Ala Asp Lys 
            100                 105                 110         


Ala Phe Val Glu Asn Arg Pro Thr Ile Leu Met Val Lys Arg Lys Pro 
        115                 120                 125             


Ile Asn Ile Asn Ile Asp Leu Pro Gly Leu Lys Gly Glu Asn Ser Ile 
    130                 135                 140                 


Lys Val Asp Asp Pro Thr Tyr Gly Lys Val Ser Gly Ala Ile Asp Glu 
145                 150                 155                 160 


Leu Val Ser Lys Trp Asn Glu Lys Tyr Ser Ser Thr His Thr Leu Pro 
                165                 170                 175     


Ala Arg Thr Gln Tyr Ser Glu Ser Met Val Tyr Ser Lys Ser Gln Ile 
            180                 185                 190         


Ser Ser Ala Leu Asn Val Asn Ala Lys Val Leu Glu Asn Ser Leu Gly 
        195                 200                 205             


Val Asp Phe Asn Ala Val Ala Asn Asn Glu Lys Lys Val Met Ile Leu 
    210                 215                 220                 


Ala Tyr Lys Gln Ile Phe Tyr Thr Val Ser Ala Asp Leu Pro Lys Asn 
225                 230                 235                 240 


Pro Ser Asp Leu Phe Asp Asp Ser Val Thr Phe Asn Asp Leu Lys Gln 
                245                 250                 255     


Lys Gly Val Ser Asn Glu Ala Pro Pro Leu Met Val Ser Asn Val Ala 
            260                 265                 270         


Tyr Gly Arg Thr Ile Tyr Val Lys Leu Glu Thr Thr Ser Ser Ser Lys 
        275                 280                 285             


Asp Val Gln Ala Ala Phe Lys Ala Leu Ile Lys Asn Thr Asp Ile Lys 
    290                 295                 300                 


Asn Ser Gln Gln Tyr Lys Asp Ile Tyr Glu Asn Ser Ser Phe Thr Ala 
305                 310                 315                 320 


Val Val Leu Gly Gly Asp Ala Gln Glu His Asn Lys Val Val Thr Lys 
                325                 330                 335     


Asp Phe Asp Glu Ile Arg Lys Val Ile Lys Asp Asn Ala Thr Phe Ser 
            340                 345                 350         


Thr Lys Asn Pro Ala Tyr Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys 
        355                 360                 365             


Asp Asn Ser Val Ala Ala Val His Asn Lys Thr Asp Tyr Ile Glu Thr 
    370                 375                 380                 


Thr Ser Thr Glu Tyr Ser Lys Gly Lys Ile Asn Leu Asp His Ser Gly 
385                 390                 395                 400 


Ala Tyr Val Ala Gln Phe Glu Val Ala Trp Asp Glu Val Ser Tyr Asp 
                405                 410                 415     


Lys Glu Gly Asn Glu Val Leu Thr His Lys Thr Trp Asp Gly Asn Tyr 
            420                 425                 430         


Gln Asp Lys Thr Ala His Tyr Ser Thr Val Ile Pro Leu Glu Ala Asn 
        435                 440                 445             


Ala Arg Asn Ile Arg Ile Lys Ala Arg Glu Cys Thr Gly Leu Ala Trp 
    450                 455                 460                 


Glu Trp Trp Arg Asp Val Ile Ser Glu Tyr Asp Val Pro Leu Thr Asn 
465                 470                 475                 480 


Asn Ile Asn Val Ser Ile Trp Gly Thr Thr Leu Tyr Pro Gly Ser Ser 
                485                 490                 495     


Ile Thr Tyr Asn 
            500 


<210>  3
<211>  500
<212>  PRT
<213>  Clostridium perfringens

<400>  3

Met Ile Arg Phe Lys Lys Thr Lys Leu Ile Ala Ser Ile Ala Met Ala 
1               5                   10                  15      


Leu Cys Leu Phe Ser Gln Pro Val Ile Ser Phe Ser Lys Asp Ile Thr 
            20                  25                  30          


Asp Lys Asn Gln Ser Ile Asp Ser Gly Ile Ser Ser Leu Ser Tyr Asn 
        35                  40                  45              


Arg Asn Glu Val Leu Ala Ser Asn Gly Asp Lys Ile Glu Ser Phe Val 
    50                  55                  60                  


Pro Lys Glu Gly Lys Lys Thr Gly Asn Lys Phe Ile Val Val Glu Arg 
65                  70                  75                  80  


Gln Lys Arg Ser Leu Thr Thr Ser Pro Val Asp Ile Ser Ile Ile Asp 
                85                  90                  95      


Ser Val Asn Asp Arg Thr Tyr Pro Gly Ala Leu Gln Leu Ala Asp Lys 
            100                 105                 110         


Ala Phe Val Glu Asn Arg Pro Thr Ile Leu Met Val Lys Arg Lys Pro 
        115                 120                 125             


Ile Asn Ile Asn Ile Asp Leu Pro Gly Leu Lys Gly Glu Asn Ser Ile 
    130                 135                 140                 


Lys Val Asp Asp Pro Thr Tyr Gly Lys Val Ser Gly Ala Ile Asp Glu 
145                 150                 155                 160 


Leu Val Ser Lys Trp Asn Glu Lys Tyr Ser Ser Thr His Thr Leu Pro 
                165                 170                 175     


Ala Arg Thr Gln Tyr Ser Glu Ser Met Val Tyr Ser Lys Ser Gln Ile 
            180                 185                 190         


Ser Ser Ala Leu Asn Val Asn Ala Lys Val Leu Glu Asn Ser Leu Gly 
        195                 200                 205             


Val Asp Phe Asn Ala Val Ala Asn Asn Glu Lys Lys Val Met Ile Leu 
    210                 215                 220                 


Ala Tyr Lys Gln Ile Phe Tyr Thr Val Ser Ala Asp Leu Pro Lys Asn 
225                 230                 235                 240 


Pro Ser Asp Leu Phe Asp Asp Ser Val Thr Phe Asn Asp Leu Lys Gln 
                245                 250                 255     


Lys Gly Val Ser Asn Glu Ala Pro Pro Leu Met Val Ser Asn Val Ala 
            260                 265                 270         


Tyr Gly Arg Thr Ile Tyr Val Lys Leu Glu Thr Thr Ser Ser Ser Lys 
        275                 280                 285             


Asp Val Gln Ala Ala Phe Lys Ala Leu Ile Lys Asn Thr Asp Ile Lys 
    290                 295                 300                 


Asn Ser Gln Gln Tyr Lys Asp Ile Tyr Glu Asn Ser Ser Phe Thr Ala 
305                 310                 315                 320 


Val Val Leu Gly Gly Asp Ala Gln Glu His Asn Lys Val Val Thr Lys 
                325                 330                 335     


Asp Phe Asp Glu Ile Arg Lys Val Ile Lys Asp Asn Ala Thr Phe Ser 
            340                 345                 350         


Thr Lys Asn Pro Ala Tyr Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys 
        355                 360                 365             


Asp Asn Ser Val Ala Ala Val His Asn Lys Thr Asp Tyr Ile Glu Thr 
    370                 375                 380                 


Thr Ser Thr Glu Tyr Ser Lys Gly Lys Ile Asn Leu Asp His Ser Gly 
385                 390                 395                 400 


Ala Tyr Val Ala Gln Phe Glu Val Ala Trp Asp Glu Val Ser Tyr Asp 
                405                 410                 415     


Lys Glu Gly Asn Glu Val Leu Thr His Lys Thr Trp Asp Gly Asn Tyr 
            420                 425                 430         


Gln Asp Lys Thr Ser His Tyr Ser Thr Val Ile Pro Leu Glu Ala Asn 
        435                 440                 445             


Val Arg Asn Ile Arg Ile Lys Ala Arg Glu Cys Thr Gly Leu Ala Trp 
    450                 455                 460                 


Glu Trp Trp Arg Asp Val Ile Ser Glu Tyr Asp Val Pro Leu Thr Asn 
465                 470                 475                 480 


Asn Ile Asn Val Ser Ile Trp Gly Thr Thr Leu Tyr Pro Gly Ser Ser 
                485                 490                 495     


Ile Thr Tyr Asn 
            500 


<210>  4
<211>  542
<212>  PRT
<213>  Clostridium perfringens

<400>  4

Met Lys Phe Lys Ile Ile Ser Leu Leu Asn Pro Ile Lys Phe Leu Lys 
1               5                   10                  15      


Phe Lys Ser Ser Ile Lys Phe Leu Gly Phe Met Ser Leu Thr Asn Glu 
            20                  25                  30          


Gly Lys Ile Lys Lys Arg Gly Ile Tyr Ile Met Ile Arg Phe Lys Lys 
        35                  40                  45              


Thr Lys Leu Ile Ala Ser Ile Ala Met Ala Leu Cys Leu Phe Ser Gln 
    50                  55                  60                  


Pro Val Ile Ser Phe Ser Lys Asp Ile Thr Asp Lys Asn Gln Ser Ile 
65                  70                  75                  80  


Asp Ser Gly Ile Ser Ser Leu Ser Tyr Asn Arg Asn Glu Val Leu Ala 
                85                  90                  95      


Ser Asn Gly Asp Lys Ile Glu Ser Phe Val Pro Lys Glu Gly Lys Lys 
            100                 105                 110         


Thr Gly Asn Lys Phe Ile Val Val Glu Arg Gln Lys Arg Ser Leu Thr 
        115                 120                 125             


Thr Ser Pro Val Asp Ile Ser Ile Ile Asp Ser Val Asn Asp Arg Thr 
    130                 135                 140                 


Tyr Pro Gly Ala Leu Gln Leu Ala Asp Lys Ala Phe Val Glu Asn Arg 
145                 150                 155                 160 


Pro Thr Ile Leu Met Val Lys Arg Lys Pro Ile Asn Ile Asn Ile Asp 
                165                 170                 175     


Leu Pro Gly Leu Lys Gly Glu Asn Ser Ile Lys Val Asp Asp Pro Thr 
            180                 185                 190         


Tyr Gly Lys Val Ser Gly Ala Ile Asp Glu Leu Val Ser Lys Trp Asn 
        195                 200                 205             


Lys Lys Tyr Ser Ser Thr His Thr Leu Pro Ala Arg Thr Gln Tyr Ser 
    210                 215                 220                 


Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Ser Ser Ala Leu Asn Val 
225                 230                 235                 240 


Asn Ala Lys Val Leu Glu Asn Ser Leu Gly Val Asp Phe Asn Ala Val 
                245                 250                 255     


Ala Asn Asn Glu Lys Lys Val Met Ile Leu Ala Tyr Lys Gln Ile Phe 
            260                 265                 270         


Tyr Thr Val Ser Ala Asp Leu Pro Lys Asn Pro Ser Asp Leu Phe Asp 
        275                 280                 285             


Asp Ser Val Thr Phe Asn Asp Leu Lys Gln Lys Gly Val Ser Asn Glu 
    290                 295                 300                 


Ala Pro Pro Leu Met Val Ser Asn Val Ala Tyr Gly Arg Thr Ile Tyr 
305                 310                 315                 320 


Val Lys Leu Glu Thr Thr Ser Ser Ser Lys Asp Val Gln Ala Ala Phe 
                325                 330                 335     


Lys Ala Leu Ile Lys Asn Thr Asp Ile Lys Asn Ser Gln Gln Tyr Lys 
            340                 345                 350         


Asp Ile Tyr Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 
        355                 360                 365             


Ala Gln Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Glu Ile Arg 
    370                 375                 380                 


Lys Val Ile Lys Asp Asn Ala Thr Phe Ser Thr Lys Asn Pro Ala Tyr 
385                 390                 395                 400 


Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asp Asn Ser Val Ala Ala 
                405                 410                 415     


Val His Asn Lys Thr Asp Tyr Ile Glu Thr Thr Ser Thr Glu Tyr Ser 
            420                 425                 430         


Lys Gly Lys Ile Asn Leu Asp His Ser Gly Ala Tyr Val Ala Gln Phe 
        435                 440                 445             


Glu Val Ala Trp Asp Glu Val Ser Tyr Asp Lys Glu Gly Asn Glu Val 
    450                 455                 460                 


Leu Thr His Lys Thr Trp Asp Gly Asn Tyr Gln Asp Lys Thr Ala His 
465                 470                 475                 480 


Tyr Ser Thr Val Ile Pro Leu Glu Ala Asn Ala Arg Asn Ile Arg Ile 
                485                 490                 495     


Lys Ala Arg Glu Cys Thr Gly Leu Ala Trp Glu Trp Trp Arg Asp Val 
            500                 505                 510         


Ile Ser Glu Tyr Asp Val Pro Leu Thr Asn Asn Ile Asn Val Ser Ile 
        515                 520                 525             


Trp Gly Thr Thr Leu Tyr Pro Gly Ser Ser Ile Thr Tyr Asn 
    530                 535                 540         


<210>  5
<211>  500
<212>  PRT
<213>  Clostridium perfringens

<400>  5

Met Ile Arg Phe Lys Lys Thr Lys Leu Ile Ala Ser Ile Ala Met Ala 
1               5                   10                  15      


Leu Cys Leu Phe Ser Gln Pro Val Ile Ser Phe Ser Lys Asp Ile Thr 
            20                  25                  30          


Asp Lys Asn Gln Ser Ile Asp Ser Gly Ile Ser Ser Leu Ser Tyr Asn 
        35                  40                  45              


Arg Asn Glu Val Leu Ala Ser Asn Gly Asp Lys Ile Glu Ser Phe Val 
    50                  55                  60                  


Pro Lys Glu Gly Lys Lys Thr Gly Asn Lys Phe Ile Val Val Glu Arg 
65                  70                  75                  80  


Gln Lys Arg Ser Leu Thr Thr Ser Pro Val Asp Ile Ser Ile Ile Asp 
                85                  90                  95      


Ser Val Asn Asp Arg Thr Tyr Pro Gly Ala Leu Gln Leu Ala Asp Lys 
            100                 105                 110         


Ala Phe Val Glu Asn Arg Pro Thr Ile Leu Met Val Lys Arg Lys Pro 
        115                 120                 125             


Ile Asn Ile Asn Ile Asp Leu Pro Gly Leu Lys Gly Glu Asn Ser Ile 
    130                 135                 140                 


Lys Val Asp Asp Pro Thr Tyr Gly Lys Val Ser Gly Ala Ile Asp Glu 
145                 150                 155                 160 


Leu Val Ser Lys Trp Asn Asp Lys Tyr Ser Ser Thr His Thr Leu Pro 
                165                 170                 175     


Ala Arg Thr Gln Tyr Ser Glu Ser Met Val Tyr Ser Lys Ser Gln Ile 
            180                 185                 190         


Ser Ser Ala Leu Asn Val Asn Ala Lys Val Leu Glu Asn Ser Leu Gly 
        195                 200                 205             


Val Asp Phe Asn Ala Val Ala Asn Asn Glu Lys Lys Val Met Ile Leu 
    210                 215                 220                 


Ala Tyr Lys Gln Ile Phe Tyr Thr Val Ser Ala Asp Leu Pro Lys Asn 
225                 230                 235                 240 


Pro Ser Asp Leu Phe Asp Asp Ser Val Thr Phe Asn Asp Leu Lys Gln 
                245                 250                 255     


Lys Gly Val Ser Asn Glu Ala Pro Pro Leu Met Val Ser Asn Val Ala 
            260                 265                 270         


Tyr Gly Arg Thr Ile Tyr Val Lys Leu Glu Thr Thr Ser Ser Ser Lys 
        275                 280                 285             


Asp Val Gln Ala Ala Phe Lys Ala Leu Ile Lys Asn Thr Asp Ile Lys 
    290                 295                 300                 


Asn Ser Gln Gln Tyr Lys Asp Ile Tyr Glu Asn Ser Ser Phe Thr Ala 
305                 310                 315                 320 


Val Val Leu Gly Gly Asp Ala Gln Glu His Asn Lys Val Val Thr Lys 
                325                 330                 335     


Asp Phe Asp Glu Ile Arg Lys Val Ile Lys Asp Asn Ala Thr Phe Ser 
            340                 345                 350         


Thr Lys Asn Pro Ala Tyr Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys 
        355                 360                 365             


Asp Asn Ser Val Ala Ala Val His Asn Lys Thr Asp Tyr Ile Glu Thr 
    370                 375                 380                 


Thr Ser Thr Glu Tyr Ser Lys Gly Lys Ile Asn Leu Asp His Ser Gly 
385                 390                 395                 400 


Ala Tyr Val Ala Gln Phe Glu Val Ala Trp Asp Glu Val Ser Tyr Asp 
                405                 410                 415     


Lys Glu Gly Asn Glu Val Leu Thr His Lys Thr Trp Asp Gly Asn Tyr 
            420                 425                 430         


Gln Asp Lys Thr Ala His Tyr Ser Thr Val Ile Pro Leu Glu Ala Asn 
        435                 440                 445             


Ala Arg Asn Ile Arg Ile Lys Ala Arg Glu Cys Thr Gly Leu Ala Trp 
    450                 455                 460                 


Glu Trp Trp Arg Asp Val Ile Ser Glu Tyr Asp Val Pro Leu Thr Asn 
465                 470                 475                 480 


Asn Ile Asn Val Ser Ile Trp Gly Thr Thr Leu Tyr Pro Gly Ser Ser 
                485                 490                 495     


Ile Thr Tyr Asn 
            500 


<210>  6
<211>  499
<212>  PRT
<213>  Clostridium perfringens

<400>  6

Met Ile Arg Phe Lys Lys Thr Lys Leu Ile Ala Ser Ile Ala Met Ala 
1               5                   10                  15      


Leu Cys Leu Phe Ser Gln Pro Val Ile Ser Phe Ser Lys Asp Ile Thr 
            20                  25                  30          


Asp Lys Asn Gln Ser Ile Asp Ser Gly Ile Ser Ser Leu Ser Tyr Asn 
        35                  40                  45              


Arg Asn Glu Val Leu Ala Ser Asn Gly Asp Lys Ile Glu Ser Phe Val 
    50                  55                  60                  


Pro Lys Glu Gly Lys Lys Ala Gly Asn Lys Phe Ile Val Val Glu Arg 
65                  70                  75                  80  


Gln Lys Arg Ser Leu Thr Thr Ser Pro Val Asp Ile Ser Ile Ile Asp 
                85                  90                  95      


Ser Val Asn Asp Arg Thr Tyr Pro Gly Ala Leu Gln Leu Ala Asp Lys 
            100                 105                 110         


Ala Phe Val Glu Asn Arg Pro Thr Ile Leu Met Val Lys Glu Ala Ile 
        115                 120                 125             


Asn Ile Asn Ile Asp Leu Pro Gly Leu Lys Gly Glu Asn Ser Ile Lys 
    130                 135                 140                 


Val Asp Asp Pro Thr Tyr Gly Lys Val Ser Gly Ala Ile Asp Glu Leu 
145                 150                 155                 160 


Val Ser Lys Trp Asn Glu Lys Tyr Ser Ser Thr His Thr Leu Pro Ala 
                165                 170                 175     


Arg Thr Gln Tyr Ser Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Ser 
            180                 185                 190         


Ser Ala Leu Asn Val Asn Ala Lys Val Leu Glu Asn Ser Leu Gly Val 
        195                 200                 205             


Asp Phe Asn Ala Val Ala Asn Asn Glu Lys Lys Val Met Ile Leu Ala 
    210                 215                 220                 


Tyr Lys Gln Ile Phe Tyr Thr Val Ser Ala Asp Leu Pro Lys Asn Pro 
225                 230                 235                 240 


Ser Asp Leu Phe Asp Asp Ser Val Thr Phe Asn Asp Leu Lys Gln Lys 
                245                 250                 255     


Gly Val Ser Asn Glu Ala Pro Pro Leu Met Val Ser Asn Val Ala Tyr 
            260                 265                 270         


Gly Arg Thr Ile Tyr Val Lys Leu Glu Thr Thr Ser Ser Ser Lys Asp 
        275                 280                 285             


Val Gln Ala Ala Phe Lys Ala Leu Ile Lys Asn Thr Asp Ile Lys Asn 
    290                 295                 300                 


Ser Gln Gln Tyr Lys Asp Ile Tyr Glu Asn Ser Ser Phe Thr Ala Val 
305                 310                 315                 320 


Val Leu Gly Gly Asp Ala Gln Glu His Asn Lys Val Val Thr Lys Asp 
                325                 330                 335     


Phe Asp Glu Ile Arg Lys Val Ile Lys Asp Asn Ala Thr Phe Ser Thr 
            340                 345                 350         


Lys Asn Pro Ala Tyr Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asp 
        355                 360                 365             


Asn Ser Val Ala Ala Val His Asn Lys Thr Asp Tyr Ile Glu Thr Thr 
    370                 375                 380                 


Ser Thr Glu Tyr Ser Lys Gly Lys Ile Asn Leu Asp His Ser Gly Ala 
385                 390                 395                 400 


Tyr Val Ala Gln Phe Glu Val Ala Trp Asp Glu Val Ser Tyr Asp Lys 
                405                 410                 415     


Glu Gly Asn Glu Val Leu Thr His Lys Thr Trp Asp Gly Asn Tyr Gln 
            420                 425                 430         


Asp Lys Thr Ala His Tyr Ser Thr Val Ile Pro Leu Glu Ala Asn Ala 
        435                 440                 445             


Arg Asn Ile Arg Ile Lys Ala Arg Glu Cys Thr Gly Leu Ala Trp Glu 
    450                 455                 460                 


Trp Trp Arg Asp Val Ile Ser Glu Tyr Asp Val Pro Leu Thr Asn Asn 
465                 470                 475                 480 


Ile Asn Val Ser Ile Trp Gly Thr Thr Leu Tyr Pro Gly Ser Ser Ile 
                485                 490                 495     


Thr Tyr Asn 
            


<210>  7
<211>  500
<212>  PRT
<213>  Clostridium perfringens

<400>  7

Met Ile Arg Phe Lys Lys Thr Lys Leu Ile Ala Ser Ile Ala Met Ala 
1               5                   10                  15      


Leu Cys Leu Phe Ser Gln Pro Val Ile Ser Phe Ser Lys Asp Ile Thr 
            20                  25                  30          


Asp Lys Asn Gln Ser Ile Asp Ser Gly Ile Ser Ser Leu Ser Tyr Asn 
        35                  40                  45              


Arg Asn Glu Val Leu Ala Ser Asn Gly Asp Lys Ile Glu Ser Phe Val 
    50                  55                  60                  


Pro Lys Glu Gly Lys Lys Ala Gly Asn Lys Phe Ile Val Val Glu Arg 
65                  70                  75                  80  


Gln Lys Arg Ser Leu Thr Thr Ser Pro Val Asp Ile Ser Ile Ile Asp 
                85                  90                  95      


Ser Val Asn Asp Arg Thr Tyr Pro Gly Ala Leu Gln Leu Ala Asp Lys 
            100                 105                 110         


Ala Leu Val Glu Asn Arg Pro Thr Ile Leu Met Val Lys Arg Lys Pro 
        115                 120                 125             


Ile Asn Ile Asn Ile Asp Leu Pro Gly Leu Lys Gly Glu Asn Ser Ile 
    130                 135                 140                 


Lys Val Asp Asp Pro Thr Tyr Gly Lys Val Ser Gly Ala Ile Asp Glu 
145                 150                 155                 160 


Leu Val Ser Lys Trp Asn Glu Lys Tyr Ser Ser Thr His Thr Leu Pro 
                165                 170                 175     


Ala Arg Thr Gln Tyr Ser Glu Ser Met Val Tyr Ser Lys Ser Gln Ile 
            180                 185                 190         


Ser Ser Ala Leu Asn Val Asn Ala Lys Val Leu Glu Asn Ser Leu Gly 
        195                 200                 205             


Val Asp Phe Asn Ala Val Ala Asn Asn Glu Lys Lys Val Met Ile Leu 
    210                 215                 220                 


Ala Tyr Lys Gln Ile Phe Tyr Thr Val Ser Ala Asp Leu Pro Lys Asn 
225                 230                 235                 240 


Pro Ser Asp Leu Phe Asp Asp Ser Val Thr Phe Asn Asp Leu Lys Gln 
                245                 250                 255     


Lys Gly Val Ser Asn Glu Ala Pro Pro Leu Met Val Ser Asn Val Ala 
            260                 265                 270         


Tyr Gly Arg Thr Ile Tyr Val Lys Leu Glu Thr Thr Ser Ser Ser Lys 
        275                 280                 285             


Asp Val Gln Ala Ala Phe Lys Ala Leu Ile Lys Asn Thr Asp Ile Lys 
    290                 295                 300                 


Asn Ser Gln Gln Tyr Lys Asp Ile Tyr Glu Asn Ser Ser Phe Thr Ala 
305                 310                 315                 320 


Val Val Leu Gly Gly Asp Ala Gln Glu His Asn Lys Val Val Thr Lys 
                325                 330                 335     


Asp Phe Asp Glu Ile Arg Lys Val Ile Lys Asp Asn Ala Thr Phe Ser 
            340                 345                 350         


Thr Lys Asn Pro Ala Tyr Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys 
        355                 360                 365             


Asp Asn Ser Val Ala Ala Val His Asn Lys Thr Asp Tyr Ile Glu Thr 
    370                 375                 380                 


Thr Ser Thr Glu Tyr Ser Lys Gly Lys Ile Asn Leu Asp His Ser Gly 
385                 390                 395                 400 


Ala Tyr Val Ala Gln Phe Glu Val Ala Trp Asp Glu Val Ser Tyr Asp 
                405                 410                 415     


Lys Glu Gly Asn Glu Val Leu Thr His Lys Thr Trp Asp Gly Asn Tyr 
            420                 425                 430         


Gln Asp Lys Thr Ala His Tyr Ser Thr Val Ile Pro Leu Glu Ala Asn 
        435                 440                 445             


Ala Arg Asn Ile Arg Ile Lys Ala Arg Glu Cys Thr Gly Leu Ala Trp 
    450                 455                 460                 


Glu Trp Trp Arg Asp Val Ile Ser Glu Tyr Asp Val Pro Leu Thr Asn 
465                 470                 475                 480 


Asn Ile Asn Val Ser Ile Trp Gly Thr Thr Leu Tyr Pro Gly Ser Ser 
                485                 490                 495     


Ile Thr Tyr Asn 
            500 


<210>  8
<211>  509
<212>  PRT
<213>  Clostridium perfringens

<400>  8

Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 
1               5                   10                  15      


Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 
            20                  25                  30          


Pro Ser Ser Arg Ser Lys Asp Ile Thr Asp Lys Asn Gln Ser Ile Asp 
        35                  40                  45              


Ser Gly Ile Ser Ser Leu Ser Tyr Asn Arg Asn Glu Val Leu Ala Ser 
    50                  55                  60                  


Asn Gly Asp Lys Ile Glu Ser Phe Val Pro Lys Glu Gly Lys Lys Ala 
65                  70                  75                  80  


Gly Asn Lys Phe Ile Val Val Glu Arg Gln Lys Arg Ser Leu Thr Thr 
                85                  90                  95      


Ser Pro Val Asp Ile Ser Ile Ile Asp Ser Val Asn Asp Arg Thr Tyr 
            100                 105                 110         


Pro Gly Ala Leu Gln Leu Ala Asp Lys Ala Phe Val Glu Asn Arg Pro 
        115                 120                 125             


Thr Ile Leu Met Val Lys Arg Lys Pro Ile Asn Ile Asn Ile Asp Leu 
    130                 135                 140                 


Pro Gly Leu Lys Gly Glu Asn Ser Ile Lys Val Asp Asp Pro Thr Tyr 
145                 150                 155                 160 


Gly Lys Val Ser Gly Ala Ile Asp Glu Leu Val Ser Lys Trp Asn Glu 
                165                 170                 175     


Lys Tyr Ser Ser Thr His Thr Leu Pro Ala Arg Thr Gln Tyr Ser Glu 
            180                 185                 190         


Ser Met Val Tyr Ser Lys Ser Gln Ile Ser Ser Ala Leu Asn Val Asn 
        195                 200                 205             


Ala Lys Val Leu Glu Asn Ser Leu Gly Val Asp Phe Asn Ala Val Ala 
    210                 215                 220                 


Asn Asn Glu Lys Lys Val Met Ile Leu Ala Tyr Lys Gln Ile Phe Tyr 
225                 230                 235                 240 


Thr Val Ser Ala Asp Leu Pro Lys Asn Pro Ser Asp Leu Phe Asp Asp 
                245                 250                 255     


Ser Val Thr Phe Asn Asp Leu Lys Gln Lys Gly Val Ser Asn Glu Ala 
            260                 265                 270         


Pro Pro Leu Met Val Ser Asn Val Ala Tyr Gly Arg Thr Ile Tyr Val 
        275                 280                 285             


Lys Leu Glu Thr Thr Ser Ser Ser Lys Asp Val Gln Ala Ala Phe Lys 
    290                 295                 300                 


Ala Leu Ile Lys Asn Thr Asp Ile Lys Asn Ser Gln Gln Tyr Lys Asp 
305                 310                 315                 320 


Ile Tyr Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp Ala 
                325                 330                 335     


Gln Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Glu Ile Arg Lys 
            340                 345                 350         


Val Ile Lys Asp Asn Ala Thr Phe Ser Thr Lys Asn Pro Ala Tyr Pro 
        355                 360                 365             


Ile Ser Tyr Thr Ser Val Phe Leu Lys Asp Asn Ser Val Ala Ala Val 
    370                 375                 380                 


His Asn Lys Thr Asp Tyr Ile Glu Thr Thr Ser Thr Glu Tyr Ser Lys 
385                 390                 395                 400 


Gly Lys Ile Asn Leu Asp His Ser Gly Ala Tyr Val Ala Gln Phe Glu 
                405                 410                 415     


Val Ala Trp Asp Glu Val Ser Tyr Asp Lys Glu Gly Asn Glu Val Leu 
            420                 425                 430         


Thr His Lys Thr Trp Asp Gly Asn Tyr Gln Asp Lys Thr Ala His Tyr 
        435                 440                 445             


Ser Thr Val Ile Pro Leu Glu Ala Asn Ala Arg Asn Ile Arg Ile Lys 
    450                 455                 460                 


Ala Arg Glu Cys Thr Gly Leu Ala Trp Glu Trp Trp Arg Asp Val Ile 
465                 470                 475                 480 


Ser Glu Tyr Asp Val Pro Leu Thr Asn Asn Ile Asn Val Ser Ile Trp 
                485                 490                 495     


Gly Thr Thr Leu Tyr Pro Gly Ser Ser Ile Thr Tyr Asn 
            500                 505                 


<210>  9
<211>  509
<212>  PRT
<213>  Clostridium perfringens

<400>  9

Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 
1               5                   10                  15      


Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 
            20                  25                  30          


Pro Ser Ser Arg Ser Lys Asp Ile Thr Asp Lys Asn Gln Ser Ile Asp 
        35                  40                  45              


Ser Gly Ile Ser Ser Leu Ser Tyr Asn Arg Asn Glu Val Leu Ala Ser 
    50                  55                  60                  


Asn Gly Asp Lys Ile Glu Ser Phe Val Pro Lys Glu Gly Lys Lys Ala 
65                  70                  75                  80  


Gly Asn Lys Phe Ile Val Val Glu Arg Gln Lys Arg Ser Leu Thr Thr 
                85                  90                  95      


Ser Pro Val Asp Ile Ser Ile Ile Asp Ser Val Asn Asp Arg Thr Tyr 
            100                 105                 110         


Pro Gly Ala Leu Gln Leu Ala Asp Lys Ala Phe Val Glu Asn Arg Pro 
        115                 120                 125             


Thr Ile Leu Met Val Lys Arg Lys Pro Ile Asn Ile Asn Ile Asp Leu 
    130                 135                 140                 


Pro Gly Leu Lys Gly Glu Asn Ser Ile Lys Val Asp Asp Pro Thr Tyr 
145                 150                 155                 160 


Gly Lys Val Ser Gly Ala Ile Asp Glu Leu Val Ser Lys Trp Asn Glu 
                165                 170                 175     


Lys Tyr Ser Ser Thr His Thr Leu Pro Ala Arg Thr Gln Tyr Ser Glu 
            180                 185                 190         


Ser Met Val Tyr Ser Lys Ser Gln Ile Ser Ser Ala Leu Asn Val Asn 
        195                 200                 205             


Ala Lys Val Leu Glu Asn Ser Leu Gly Val Asp Phe Asn Ala Val Ala 
    210                 215                 220                 


Asn Asn Glu Lys Lys Val Met Ile Leu Ala Tyr Lys Gln Ile Phe Tyr 
225                 230                 235                 240 


Thr Val Ser Ala Asp Leu Pro Lys Asn Pro Ser Asp Leu Phe Asp Asp 
                245                 250                 255     


Ser Val Thr Phe Asn Asp Leu Lys Gln Lys Gly Val Ser Asn Glu Ala 
            260                 265                 270         


Pro Pro Leu Met Val Ser Asn Val Ala Tyr Gly Arg Thr Ile Tyr Val 
        275                 280                 285             


Lys Leu Glu Thr Thr Ser Ser Ser Lys Asp Val Gln Ala Ala Phe Lys 
    290                 295                 300                 


Ala Leu Ile Lys Asn Thr Asp Ile Lys Asn Ser Gln Gln Tyr Lys Asp 
305                 310                 315                 320 


Ile Tyr Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp Ala 
                325                 330                 335     


Gln Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Glu Ile Arg Lys 
            340                 345                 350         


Val Ile Lys Asp Asn Ala Thr Phe Ser Thr Lys Asn Pro Ala Tyr Pro 
        355                 360                 365             


Ile Ser Tyr Thr Ser Val Phe Leu Lys Asp Asn Ser Val Ala Ala Val 
    370                 375                 380                 


His Asn Lys Thr Asp Tyr Ile Glu Thr Thr Ser Thr Glu Tyr Ser Lys 
385                 390                 395                 400 


Gly Lys Ile Asn Leu Asp His Ser Gly Ala Tyr Val Ala Gln Phe Glu 
                405                 410                 415     


Val Ala Trp Asp Glu Val Ser Tyr Asp Lys Glu Gly Asn Glu Val Leu 
            420                 425                 430         


Thr His Lys Thr Trp Asp Gly Asn Tyr Gln Asp Lys Thr Ala His Tyr 
        435                 440                 445             


Ser Thr Val Ile Pro Leu Glu Ala Asn Ala Arg Asn Ile Arg Ile Lys 
    450                 455                 460                 


Ala Arg Glu Ala Thr Gly Leu Ala Trp Glu Trp Trp Arg Asp Val Ile 
465                 470                 475                 480 


Ser Glu Tyr Asp Val Pro Leu Thr Asn Asn Ile Asn Val Ser Ile Trp 
                485                 490                 495     


Gly Thr Thr Leu Tyr Pro Gly Ser Ser Ile Thr Tyr Asn 
            500                 505                 


<210>  10
<211>  509
<212>  PRT
<213>  Clostridium perfringens

<400>  10

Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 
1               5                   10                  15      


Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 
            20                  25                  30          


Pro Ser Ser Arg Ser Lys Asp Ile Thr Asp Lys Asn Gln Ser Ile Asp 
        35                  40                  45              


Ser Gly Ile Ser Ser Leu Ser Tyr Asn Arg Asn Glu Val Leu Ala Ser 
    50                  55                  60                  


Asn Gly Asp Lys Ile Glu Ser Phe Val Pro Lys Glu Gly Lys Lys Ala 
65                  70                  75                  80  


Gly Asn Lys Phe Ile Val Val Glu Arg Gln Lys Arg Ser Leu Thr Thr 
                85                  90                  95      


Ser Pro Val Asp Ile Ser Ile Ile Asp Ser Val Asn Asp Arg Thr Tyr 
            100                 105                 110         


Pro Gly Ala Leu Gln Leu Ala Asp Lys Ala Phe Val Glu Asn Arg Pro 
        115                 120                 125             


Thr Ile Leu Met Val Lys Arg Lys Pro Ile Asn Ile Asn Ile Asp Leu 
    130                 135                 140                 


Pro Gly Leu Lys Gly Glu Asn Ser Ile Lys Val Asp Asp Pro Thr Tyr 
145                 150                 155                 160 


Gly Lys Val Ser Gly Ala Ile Asp Glu Leu Val Ser Lys Trp Asn Glu 
                165                 170                 175     


Lys Tyr Ser Ser Thr His Thr Leu Pro Ala Arg Thr Gln Tyr Ser Glu 
            180                 185                 190         


Ser Met Val Tyr Ser Lys Ser Gln Ile Ser Ser Ala Leu Asn Val Asn 
        195                 200                 205             


Ala Lys Val Leu Glu Asn Ser Leu Gly Val Asp Phe Asn Ala Val Ala 
    210                 215                 220                 


Asn Asn Glu Lys Lys Val Met Ile Leu Ala Tyr Lys Gln Ile Phe Tyr 
225                 230                 235                 240 


Thr Val Ser Ala Asp Leu Pro Lys Asn Pro Ser Asp Leu Phe Asp Asp 
                245                 250                 255     


Ser Val Thr Phe Asn Asp Leu Lys Gln Lys Gly Val Ser Asn Glu Ala 
            260                 265                 270         


Pro Pro Leu Met Val Ser Asn Val Ala Tyr Gly Arg Thr Ile Tyr Val 
        275                 280                 285             


Lys Leu Glu Thr Thr Ser Ser Ser Lys Asp Val Gln Ala Ala Phe Lys 
    290                 295                 300                 


Ala Leu Ile Lys Asn Thr Asp Ile Lys Asn Ser Gln Gln Tyr Lys Asp 
305                 310                 315                 320 


Ile Tyr Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp Ala 
                325                 330                 335     


Gln Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Glu Ile Arg Lys 
            340                 345                 350         


Val Ile Lys Asp Asn Ala Thr Phe Ser Thr Lys Asn Pro Ala Tyr Pro 
        355                 360                 365             


Ile Ser Tyr Thr Ser Val Phe Leu Lys Asp Asn Ser Val Ala Ala Val 
    370                 375                 380                 


His Asn Lys Thr Asp Tyr Ile Glu Thr Thr Ser Thr Glu Tyr Ser Lys 
385                 390                 395                 400 


Gly Lys Ile Asn Leu Asp His Ser Gly Ala Tyr Val Ala Gln Phe Glu 
                405                 410                 415     


Val Ala Trp Asp Glu Val Ser Tyr Asp Lys Glu Gly Asn Glu Val Leu 
            420                 425                 430         


Thr His Lys Thr Trp Asp Gly Asn Tyr Gln Ser Lys Thr Ala His Tyr 
        435                 440                 445             


Ser Thr Val Ile Pro Leu Glu Ala Asn Ala Arg Asn Ile Arg Ile Lys 
    450                 455                 460                 


Ala Arg Glu Ala Thr Gly Leu Ala Trp Glu Trp Trp Arg Asp Val Ile 
465                 470                 475                 480 


Ser Glu Tyr Asp Val Pro Leu Thr Asn Asn Ile Asn Val Ser Ile Trp 
                485                 490                 495     


Gly Thr Thr Leu Tyr Pro Gly Ser Ser Ile Thr Tyr Asn 
            500                 505                 


<210>  11
<211>  509
<212>  PRT
<213>  Clostridium perfringens

<400>  11

Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 
1               5                   10                  15      


Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 
            20                  25                  30          


Pro Ser Ser Arg Ser Lys Asp Ile Thr Asp Lys Asn Gln Ser Ile Asp 
        35                  40                  45              


Ser Gly Ile Ser Ser Leu Ser Tyr Asn Arg Asn Glu Val Leu Ala Ser 
    50                  55                  60                  


Asn Gly Asp Lys Ile Glu Ser Phe Val Pro Lys Glu Gly Lys Lys Ala 
65                  70                  75                  80  


Gly Asn Lys Phe Ile Val Val Glu Arg Gln Lys Arg Ser Leu Thr Thr 
                85                  90                  95      


Ser Pro Val Asp Ile Ser Ile Ile Asp Ser Val Asn Asp Arg Thr Tyr 
            100                 105                 110         


Pro Gly Ala Leu Gln Leu Ala Asp Lys Ala Phe Val Glu Asn Arg Pro 
        115                 120                 125             


Thr Ile Leu Met Val Lys Arg Lys Pro Ile Asn Ile Asn Ile Asp Leu 
    130                 135                 140                 


Pro Gly Leu Lys Gly Glu Asn Ser Ile Lys Val Asp Asp Pro Thr Tyr 
145                 150                 155                 160 


Gly Lys Val Ser Gly Ala Ile Asp Glu Leu Val Ser Lys Trp Asn Glu 
                165                 170                 175     


Lys Tyr Ser Ser Thr His Thr Leu Pro Ala Arg Thr Gln Tyr Ser Glu 
            180                 185                 190         


Ser Met Val Tyr Ser Lys Ser Gln Ile Ser Ser Ala Leu Asn Val Asn 
        195                 200                 205             


Ala Lys Val Leu Glu Asn Ser Leu Gly Val Asp Phe Asn Ala Val Ala 
    210                 215                 220                 


Asn Asn Glu Lys Lys Val Met Ile Leu Ala Tyr Lys Gln Ile Phe Tyr 
225                 230                 235                 240 


Thr Val Ser Ala Asp Leu Pro Lys Asn Pro Ser Asp Leu Phe Asp Asp 
                245                 250                 255     


Ser Val Thr Phe Asn Asp Leu Lys Gln Lys Gly Val Ser Asn Glu Ala 
            260                 265                 270         


Pro Pro Leu Met Val Ser Asn Val Ala Tyr Gly Arg Thr Ile Tyr Val 
        275                 280                 285             


Lys Leu Glu Thr Thr Ser Ser Ser Lys Asp Val Gln Ala Ala Phe Lys 
    290                 295                 300                 


Ala Leu Ile Lys Asn Thr Asp Ile Lys Asn Ser Gln Gln Tyr Lys Asp 
305                 310                 315                 320 


Ile Tyr Glu Asn Ser Ser Phe Cys Ala Val Val Leu Gly Gly Asp Ala 
                325                 330                 335     


Gln Glu His Asn Lys Val Cys Thr Lys Asp Phe Asp Glu Ile Arg Lys 
            340                 345                 350         


Val Ile Lys Asp Asn Ala Thr Phe Ser Thr Lys Asn Pro Ala Tyr Pro 
        355                 360                 365             


Ile Ser Tyr Thr Ser Val Phe Leu Lys Asp Asn Ser Val Ala Ala Val 
    370                 375                 380                 


His Asn Lys Thr Asp Tyr Ile Glu Thr Thr Ser Thr Glu Tyr Ser Lys 
385                 390                 395                 400 


Gly Lys Ile Asn Leu Asp His Ser Gly Ala Tyr Val Ala Gln Phe Glu 
                405                 410                 415     


Val Ala Trp Asp Glu Val Ser Tyr Asp Lys Glu Gly Asn Glu Val Leu 
            420                 425                 430         


Thr His Lys Thr Trp Asp Gly Asn Tyr Gln Asp Lys Thr Ala His Tyr 
        435                 440                 445             


Ser Thr Val Ile Pro Leu Glu Ala Asn Ala Arg Asn Ile Arg Ile Lys 
    450                 455                 460                 


Ala Arg Glu Ala Thr Gly Leu Ala Trp Glu Trp Trp Arg Asp Val Ile 
465                 470                 475                 480 


Ser Glu Tyr Asp Val Pro Leu Thr Asn Asn Ile Asn Val Ser Ile Trp 
                485                 490                 495     


Gly Thr Thr Leu Tyr Pro Gly Ser Ser Ile Thr Tyr Asn 
            500                 505                 


<210>  12
<211>  509
<212>  PRT
<213>  Clostridium perfringens

<400>  12

Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 
1               5                   10                  15      


Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 
            20                  25                  30          


Pro Ser Ser Arg Ser Lys Asp Ile Thr Asp Lys Asn Gln Ser Ile Asp 
        35                  40                  45              


Ser Gly Ile Ser Ser Leu Ser Tyr Asn Arg Asn Glu Val Leu Ala Ser 
    50                  55                  60                  


Asn Gly Asp Lys Ile Glu Ser Phe Val Pro Lys Glu Gly Lys Lys Ala 
65                  70                  75                  80  


Gly Asn Lys Phe Ile Val Val Glu Arg Gln Lys Arg Ser Leu Thr Thr 
                85                  90                  95      


Ser Pro Val Asp Ile Ser Ile Ile Asp Ser Val Asn Asp Arg Thr Tyr 
            100                 105                 110         


Pro Gly Ala Leu Gln Leu Ala Asp Lys Ala Phe Val Glu Asn Arg Pro 
        115                 120                 125             


Thr Ile Leu Met Val Lys Arg Lys Pro Ile Asn Ile Asn Ile Asp Leu 
    130                 135                 140                 


Pro Gly Leu Lys Gly Glu Asn Ser Ile Lys Val Asp Asp Pro Thr Tyr 
145                 150                 155                 160 


Gly Lys Val Ser Gly Ala Ile Asp Glu Leu Val Ser Lys Trp Asn Glu 
                165                 170                 175     


Lys Tyr Ser Ser Thr His Thr Leu Pro Ala Arg Thr Gln Tyr Ser Glu 
            180                 185                 190         


Ser Met Val Tyr Ser Lys Ser Gln Ile Ser Ser Ala Leu Asn Val Asn 
        195                 200                 205             


Ala Lys Val Leu Glu Asn Ser Leu Gly Val Asp Phe Asn Ala Val Ala 
    210                 215                 220                 


Asn Asn Glu Lys Lys Val Met Ile Leu Ala Tyr Lys Gln Ile Phe Tyr 
225                 230                 235                 240 


Thr Val Ser Ala Asp Leu Pro Lys Asn Pro Ser Asp Leu Phe Asp Asp 
                245                 250                 255     


Ser Val Thr Phe Asn Asp Leu Lys Gln Lys Gly Val Ser Asn Glu Ala 
            260                 265                 270         


Pro Pro Leu Met Val Ser Asn Val Ala Tyr Gly Arg Thr Ile Tyr Val 
        275                 280                 285             


Lys Leu Glu Thr Thr Ser Ser Ser Lys Asp Val Gln Ala Ala Phe Lys 
    290                 295                 300                 


Ala Leu Ile Lys Asn Thr Asp Ile Lys Asn Ser Gln Gln Tyr Lys Asp 
305                 310                 315                 320 


Ile Tyr Glu Asn Ser Ser Phe Cys Ala Val Val Leu Gly Gly Asp Ala 
                325                 330                 335     


Gln Glu His Asn Lys Val Cys Thr Lys Asp Phe Asp Glu Ile Arg Lys 
            340                 345                 350         


Val Ile Lys Asp Asn Ala Thr Phe Ser Thr Lys Asn Pro Ala Tyr Pro 
        355                 360                 365             


Ile Ser Tyr Thr Ser Val Phe Leu Lys Asp Asn Ser Val Ala Ala Val 
    370                 375                 380                 


His Asn Lys Thr Asp Tyr Ile Glu Thr Thr Ser Thr Glu Tyr Ser Lys 
385                 390                 395                 400 


Gly Lys Ile Asn Leu Asp His Ser Gly Ala Tyr Val Ala Gln Phe Glu 
                405                 410                 415     


Val Ala Trp Asp Glu Val Ser Tyr Asp Lys Glu Gly Asn Glu Val Leu 
            420                 425                 430         


Thr His Lys Thr Trp Asp Gly Asn Tyr Gln Ser Lys Thr Ala His Tyr 
        435                 440                 445             


Ser Thr Val Ile Pro Leu Glu Ala Asn Ala Arg Asn Ile Arg Ile Lys 
    450                 455                 460                 


Ala Arg Glu Ala Thr Gly Leu Ala Trp Glu Trp Trp Arg Asp Val Ile 
465                 470                 475                 480 


Ser Glu Tyr Asp Val Pro Leu Thr Asn Asn Ile Asn Val Ser Ile Trp 
                485                 490                 495     


Gly Thr Thr Leu Tyr Pro Gly Ser Ser Ile Thr Tyr Asn 
            500                 505                 


