                         SEQUENCE LISTING

<110>  WONG, SUI-LAM
 
<120>  TETRAMERIC STREPTAVIDIN MUTEIN WITH REVERSIBLE BIOTIN BINDING 
       CAPABILITY

<130>  UNTI.P0120WO

<140>  UNKNOWN
<141>  20120727

<150>  61/514,325
<151>  2011-08-02

<150>  13/272,922
<151>  2011-10-13

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  159
<212>  PRT
<213>  Bacillus subtilis

<400>  1

Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly 
1               5                   10                  15      


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
            20                  25                  30          


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Gly 
        35                  40                  45              


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
            100                 105                 110         


Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys 
    130                 135                 140                 


Lys Ala Gly Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
145                 150                 155                 


<210>  2
<211>  477
<212>  DNA
<213>  Bacillus subtilis

<400>  2
gacccgagca aagattctaa agcacaagta tctgctgcag aagcaggaat tacaggcaca       60

tggtataatc agctgggatc tacatttatt gttacagccg gcgcagatgg agctcttaca      120

ggaacatatg aatctgctgt tggaaatgca gaatctagat acgtgcttac aggaagatat      180

gattctgcac ctgcaacaga tggatccgga acagcacttg gatggacagt tgcatggaaa      240

aacaattata gaaacgcaca tagcgctaca acatggtctg gccaatatgt gggaggtgca      300

gaagcaagaa ttaacacaca atggctttta acatctggaa caacagaagc aaatgcatgg      360

aaaagtactc ttgttggaca tgatacattt acaaaagtta aacctagcgc agcatctatc      420

gatgcagcga aaaaagcagg agttaacaat ggcaatcctt tagatgcagt tcaacaa         477


<210>  3
<211>  188
<212>  PRT
<213>  Bacillus subtilis

<400>  3

Met Asn Ile Lys Lys Phe Ala Lys Gln Ala Thr Val Leu Thr Phe Thr 
1               5                   10                  15      


Thr Ala Leu Leu Ala Gly Gly Ala Thr Gln Ala Phe Ala Asp Pro Ser 
            20                  25                  30          


Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly Ile Thr Gly 
        35                  40                  45              


Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr Ala Gly Ala 
    50                  55                  60                  


Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Gly Asn Ala Glu 
65                  70                  75                  80  


Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro Ala Thr Asp 
                85                  90                  95      


Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys Asn Asn Tyr 
            100                 105                 110         


Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr Val Gly Gly 
        115                 120                 125             


Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser Gly Asp Ser 
    130                 135                 140                 


Ser Asn Gly Ser Asp Gly Ser Thr Leu Val Gly His Asp Thr Phe Thr 
145                 150                 155                 160 


Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys Lys Ala Gly 
                165                 170                 175     


Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
            180                 185             


<210>  4
<211>  564
<212>  DNA
<213>  Bacillus subtilis

<400>  4
atgaatatca aaaaattcgc taagcaagct acagtcctta catttacaac agcgctgctt       60

gccgggggcg ccacccaggc ctttgctgac ccgagcaaag attctaaagc acaagtatct      120

gctgcagaag cgggcattac gggcacgtgg tataatcagc tgggcagcac gtttattgtt      180

acggccggcg cagatggagc tctgacgggc acgtatgaaa gcgcggttgg caatgcagaa      240

tctagatacg ttcttacagg aagatatgat tctgcacctg caacagatgg atccggcacg      300

gcactgggct ggacagttgc atggaaaaac aattatcgca acgcacatag cgccacgacg      360

tggtctggcc aatatgttgg cggtgcagaa gcacgcatta acacacagtg gcttctgacg      420

tccggagata gcagcaatgg cagcgatggc agtactcttg ttggacatga tacatttaca      480

aaagttaaac ctagcgcagc atctatcgat gcagcgaaaa aagcaggagt taacaatggc      540

aatcctttag atgcagttca acaa                                             564


<210>  5
<211>  159
<212>  PRT
<213>  Bacillus subtilis

<400>  5

Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly 
1               5                   10                  15      


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
            20                  25                  30          


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Gly 
        35                  40                  45              


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
            100                 105                 110         


Gly Asp Ser Ser Asn Gly Ser Asp Gly Ser Thr Leu Val Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys 
    130                 135                 140                 


Lys Ala Gly Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
145                 150                 155                 


<210>  6
<211>  477
<212>  DNA
<213>  Bacillus subtilis

<400>  6
gacccgagca aagattctaa agcacaagta tctgctgcag aagcgggcat tacgggcacg       60

tggtataatc agctgggcag cacgtttatt gttacggccg gcgcagatgg agctctgacg      120

ggcacgtatg aaagcgcggt tggcaatgca gaatctagat acgttcttac aggaagatat      180

gattctgcac ctgcaacaga tggatccggc acggcactgg gctggacagt tgcatggaaa      240

aacaattatc gcaacgcaca tagcgccacg acgtggtctg gccaatatgt tggcggtgca      300

gaagcacgca ttaacacaca gtggcttctg acgtccggag atagcagcaa tggcagcgat      360

ggcagtactc ttgttggaca tgatacattt acaaaagtta aacctagcgc agcatctatc      420

gatgcagcga aaaaagcagg agttaacaat ggcaatcctt tagatgcagt tcaacaa         477


<210>  7
<211>  127
<212>  PRT
<213>  Bacillus subtilis

<400>  7

Ala Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr 
1               5                   10                  15      


Phe Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu 
            20                  25                  30          


Ser Ala Val Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr 
        35                  40                  45              


Asp Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr 
    50                  55                  60                  


Val Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp 
65                  70                  75                  80  


Ser Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp 
                85                  90                  95      


Leu Leu Thr Ser Gly Asp Ser Ser Asn Gly Ser Asp Gly Ser Thr Leu 
            100                 105                 110         


Val Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125         


<210>  8
<211>  381
<212>  DNA
<213>  Bacillus subtilis

<400>  8
gcagaagcgg gcattacggg cacgtggtat aatcagctgg gcagcacgtt tattgttacg       60

gccggcgcag atggagctct gacgggcacg tatgaaagcg cggttggcaa tgcagaatct      120

agatacgttc ttacaggaag atatgattct gcacctgcaa cagatggatc cggcacggca      180

ctgggctgga cagttgcatg gaaaaacaat tatcgcaacg cacatagcgc cacgacgtgg      240

tctggccaat atgttggcgg tgcagaagca cgcattaaca cacagtggct tctgacgtcc      300

ggagatagca gcaatggcag cgatggcagt actcttgttg gacatgatac atttacaaaa      360

gttaaaccta gcgcagcatc t                                                381


<210>  9
<211>  188
<212>  PRT
<213>  Bacillus subtilis

<400>  9

Met Asn Ile Lys Lys Phe Ala Lys Gln Ala Thr Val Leu Thr Phe Thr 
1               5                   10                  15      


Thr Ala Leu Leu Ala Gly Gly Ala Thr Gln Ala Phe Ala Asp Pro Ser 
            20                  25                  30          


Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly Ile Thr Gly 
        35                  40                  45              


Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr Ala Gly Ala 
    50                  55                  60                  


Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Gly Asn Ala Glu 
65                  70                  75                  80  


Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro Ala Thr Asp 
                85                  90                  95      


Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys Asn Asn Tyr 
            100                 105                 110         


Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr Val Gly Gly 
        115                 120                 125             


Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser Gly Asp Ser 
    130                 135                 140                 


Ser Asn Gly Ser Asp Gly Ser Thr Leu Val Gly Cys Asp Thr Phe Thr 
145                 150                 155                 160 


Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys Lys Ala Gly 
                165                 170                 175     


Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
            180                 185             


<210>  10
<211>  579
<212>  DNA
<213>  Bacillus subtilis

<400>  10
atgaatatca aaaaattcgc taagcaagct acagtcctta catttacaac agcgctgctt       60

gccgggggcg ccacccaggc ctttgctgac ccgagcaaag attctaaagc acaagtatct      120

gctgcagaag cgggcattac gggcacgtgg tataatcagc tgggcagcac gtttattgtt      180

acggccggcg cagatggagc tctgacgggc acgtatgaaa gcgcggttgg caatgcagaa      240

tctagatacg ttcttacagg aagatatgat tctgcacctg caacagatgg atccggcacg      300

gcactgggct ggacagttgc atggaaaaac aattatcgca acgcacatag cgccacgacg      360

tggtctggcc aatatgttgg cggtgcagaa gcacgcatta acacacagtg gcttctgacg      420

tccggagata gcagcaatgg cagcgatggc agtactcttg ttggatgcga tacatttaca      480

aaagttaaac ctagcgcagc atctatcgat gcagcgaaaa aagcaggagt taacaatggc      540

aatcctttag atgcagttca acaataatga tcagatatc                             579


