                         SEQUENCE LISTING

<110>  LOFARMA S.P.A.
 
<120>  Hypoallergenic variations of Mal d 1, the major allergen from Malus domestica

<130>  1437PCT

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  158
<212>  PRT
<213>  Malus domestica

<400>  1

Gly Val Tyr Thr Phe Glu Asn Glu Phe Thr Ser Glu Ile Pro Pro Ser 
1               5                   10                  15      


Arg Leu Phe Lys Ala Phe Val Leu Asp Ala Asp Asn Leu Ile Pro Lys 
            20                  25                  30          


Ile Ala Pro Gln Ala Ile Lys Gln Ala Glu Ile Leu Glu Gly Asp Gly 
        35                  40                  45              


Gly Pro Gly Thr Ile Lys Lys Ile Thr Phe Gly Glu Gly Ser Gln Tyr 
    50                  55                  60                  


Gly Tyr Val Lys His Lys Ile Asp Ser Val Asp Glu Ala Asn Tyr Ser 
65                  70                  75                  80  


Tyr Ala Tyr Thr Leu Ile Glu Gly Asp Ala Leu Thr Asp Thr Ile Glu 
                85                  90                  95      


Lys Val Ser Tyr Glu Thr Lys Leu Val Ala Ser Gly Ser Gly Ser Ile 
            100                 105                 110         


Ile Lys Ser Ile Ser His Tyr His Thr Lys Gly Asp Val Glu Ile Lys 
        115                 120                 125             


Glu Glu His Val Lys Ala Gly Lys Glu Lys Ala His Gly Leu Phe Lys 
    130                 135                 140                 


Leu Ile Glu Ser Tyr Leu Lys Gly His Pro Asp Ala Tyr Asn 
145                 150                 155             


<210>  2
<211>  158
<212>  PRT
<213>  Unknown

<220>
<223>  malus domestica - mutant

<400>  2

Gly Val Tyr Thr Phe Glu Asn Glu Phe Thr Ser Glu Ile Pro Pro Ser 
1               5                   10                  15      


Arg Leu Phe Lys Ala Phe Val Leu Ala Ala Asp Asn Leu Ile Pro Lys 
            20                  25                  30          


Ile Ala Pro Gln Ala Ile Lys Gln Ala Glu Ile Leu Glu Gly Asp Gly 
        35                  40                  45              


Gly Pro Gly Thr Ile Lys Lys Ile Thr Phe Gly Glu Gly Ser Gln Tyr 
    50                  55                  60                  


Gly Tyr Val Lys His Lys Ile Asp Ser Val Asp Glu Ala Ala Tyr Ser 
65                  70                  75                  80  


Tyr Ala Tyr Thr Leu Ile Glu Gly Asp Ala Leu Thr Asp Thr Ile Glu 
                85                  90                  95      


Lys Val Ser Tyr Glu Thr Lys Leu Val Ala Ser Gly Ser Gly Ser Ile 
            100                 105                 110         


Ile Lys Ser Ile Ser His Tyr His Thr Lys Gly Asp Val Glu Ile Lys 
        115                 120                 125             


Glu Glu His Val Lys Ala Gly Lys Glu Lys Ala His Gly Leu Phe Lys 
    130                 135                 140                 


Leu Ile Glu Ser Tyr Leu Lys Gly His Pro Asp Ala Tyr Asn 
145                 150                 155             


<210>  3
<211>  319
<212>  PRT
<213>  Unknown

<220>
<223>  Betula verrucosa - malus domestica hybrid wt

<400>  3

Gly Val Phe Asn Tyr Glu Thr Glu Thr Thr Ser Val Ile Pro Ala Ala 
1               5                   10                  15      


Arg Leu Phe Lys Ala Phe Ile Leu Asp Gly Asp Asn Leu Phe Pro Lys 
            20                  25                  30          


Val Ala Pro Gln Ala Ile Ser Ser Val Glu Asn Ile Glu Gly Asn Gly 
        35                  40                  45              


Gly Pro Gly Thr Ile Lys Lys Ile Ser Phe Pro Glu Gly Phe Pro Phe 
    50                  55                  60                  


Lys Tyr Val Lys Asp Arg Val Asp Glu Val Asp His Thr Asn Phe Lys 
65                  70                  75                  80  


Tyr Asn Tyr Ser Val Ile Glu Gly Gly Pro Ile Gly Asp Thr Leu Glu 
                85                  90                  95      


Lys Ile Ser Asn Glu Ile Lys Ile Val Ala Thr Pro Asp Gly Gly Ser 
            100                 105                 110         


Ile Leu Lys Ile Asn Asn Lys Tyr His Thr Lys Gly Asp His Glu Val 
        115                 120                 125             


Lys Ala Glu Gln Ile Lys Ala Ser Lys Glu Met Gly Glu Thr Leu Leu 
    130                 135                 140                 


Arg Ala Val Glu Ser Tyr Leu Leu Ala His Ser Asp Ala Tyr Asn Glu 
145                 150                 155                 160 


Phe Gly Val Tyr Thr Phe Glu Asn Glu Phe Thr Ser Glu Ile Pro Pro 
                165                 170                 175     


Ser Arg Leu Phe Lys Ala Phe Val Leu Asp Ala Asp Asn Leu Ile Pro 
            180                 185                 190         


Lys Ile Ala Pro Gln Ala Ile Lys Gln Ala Glu Ile Leu Glu Gly Asp 
        195                 200                 205             


Gly Gly Pro Gly Thr Ile Lys Lys Ile Thr Phe Gly Glu Gly Ser Gln 
    210                 215                 220                 


Tyr Gly Tyr Val Lys His Lys Ile Asp Ser Val Asp Glu Ala Asn Tyr 
225                 230                 235                 240 


Ser Tyr Ala Tyr Thr Leu Ile Glu Gly Asp Ala Leu Thr Asp Thr Ile 
                245                 250                 255     


Glu Lys Val Ser Tyr Glu Thr Lys Leu Val Ala Ser Gly Ser Gly Ser 
            260                 265                 270         


Ile Ile Lys Ser Ile Ser His Tyr His Thr Lys Gly Asp Val Glu Ile 
        275                 280                 285             


Lys Glu Glu His Val Lys Ala Gly Lys Glu Lys Ala His Gly Leu Phe 
    290                 295                 300                 


Lys Leu Ile Glu Ser Tyr Leu Lys Gly His Pro Asp Ala Tyr Asn 
305                 310                 315                 


<210>  4
<211>  319
<212>  PRT
<213>  Unknown

<220>
<223>  betula verrucosa - malus domestica hybrid mutant

<400>  4

Gly Val Phe Asn Tyr Glu Thr Glu Thr Thr Ser Val Ile Pro Ala Ala 
1               5                   10                  15      


Arg Leu Phe Lys Ala Phe Ile Leu Asp Gly Asp Asn Leu Phe Pro Lys 
            20                  25                  30          


Val Ala Pro Gln Ala Ile Ser Ser Val Glu Asn Ile Glu Gly Asn Gly 
        35                  40                  45              


Gly Pro Gly Thr Ile Ala Lys Ile Ser Phe Pro Glu Gly Phe Pro Phe 
    50                  55                  60                  


Lys Tyr Val Lys Asp Arg Val Asp Glu Val Asp His Thr Asn Phe Lys 
65                  70                  75                  80  


Tyr Asn Tyr Ser Val Ile Glu Gly Gly Pro Ile Gly Asp Thr Leu Glu 
                85                  90                  95      


Lys Ile Ser Asn Glu Ile Lys Ile Val Ala Thr Pro Asp Gly Gly Ser 
            100                 105                 110         


Ile Leu Ala Ile Asn Asn Lys Tyr His Thr Ala Gly Asp His Glu Val 
        115                 120                 125             


Lys Ala Glu Gln Ile Lys Ala Ser Lys Glu Met Gly Glu Thr Leu Leu 
    130                 135                 140                 


Arg Ala Val Glu Ser Tyr Leu Leu Ala His Ser Asp Ala Tyr Asn Glu 
145                 150                 155                 160 


Phe Gly Val Tyr Thr Phe Glu Asn Glu Phe Thr Ser Glu Ile Pro Pro 
                165                 170                 175     


Ser Arg Leu Phe Lys Ala Phe Val Leu Ala Ala Asp Asn Leu Ile Pro 
            180                 185                 190         


Lys Ile Ala Pro Gln Ala Ile Lys Gln Ala Glu Ile Leu Glu Gly Asp 
        195                 200                 205             


Gly Gly Pro Gly Thr Ile Lys Lys Ile Thr Phe Gly Glu Gly Ser Gln 
    210                 215                 220                 


Tyr Gly Tyr Val Lys His Lys Ile Asp Ser Val Asp Glu Ala Ala Tyr 
225                 230                 235                 240 


Ser Tyr Ala Tyr Thr Leu Ile Glu Gly Asp Ala Leu Thr Asp Thr Ile 
                245                 250                 255     


Glu Lys Val Ser Tyr Glu Thr Lys Leu Val Ala Ser Gly Ser Gly Ser 
            260                 265                 270         


Ile Ile Lys Ser Ile Ser His Tyr His Thr Lys Gly Asp Val Glu Ile 
        275                 280                 285             


Lys Glu Glu His Val Lys Ala Gly Lys Glu Lys Ala His Gly Leu Phe 
    290                 295                 300                 


Lys Leu Ile Glu Ser Tyr Leu Lys Gly His Pro Asp Ala Tyr Asn 
305                 310                 315                 


<210>  5
<211>  159
<212>  PRT
<213>  betula verrucosa

<400>  5

Gly Val Phe Asn Tyr Glu Thr Glu Thr Thr Ser Val Ile Pro Ala Ala 
1               5                   10                  15      


Arg Leu Phe Lys Ala Phe Ile Leu Asp Gly Asp Asn Leu Phe Pro Lys 
            20                  25                  30          


Val Ala Pro Gln Ala Ile Ser Ser Val Glu Asn Ile Glu Gly Asn Gly 
        35                  40                  45              


Gly Pro Gly Thr Ile Lys Lys Ile Ser Phe Pro Glu Gly Phe Pro Phe 
    50                  55                  60                  


Lys Tyr Val Lys Asp Arg Val Asp Glu Val Asp His Thr Asn Phe Lys 
65                  70                  75                  80  


Tyr Asn Tyr Ser Val Ile Glu Gly Gly Pro Ile Gly Asp Thr Leu Glu 
                85                  90                  95      


Lys Ile Ser Asn Glu Ile Lys Ile Val Ala Thr Pro Asp Gly Gly Ser 
            100                 105                 110         


Ile Leu Lys Ile Asn Asn Lys Tyr His Thr Lys Gly Asp His Glu Val 
        115                 120                 125             


Lys Ala Glu Gln Ile Lys Ala Ser Lys Glu Met Gly Glu Thr Leu Leu 
    130                 135                 140                 


Arg Ala Val Glu Ser Tyr Leu Leu Ala His Ser Asp Ala Tyr Asn 
145                 150                 155                 


<210>  6
<211>  159
<212>  PRT
<213>  Unknown

<220>
<223>  betula verrucosa - mutant

<400>  6

Gly Val Phe Asn Tyr Glu Thr Glu Thr Thr Ser Val Ile Pro Ala Ala 
1               5                   10                  15      


Arg Leu Phe Lys Ala Phe Ile Leu Asp Gly Asp Asn Leu Phe Pro Lys 
            20                  25                  30          


Val Ala Pro Gln Ala Ile Ser Ser Val Glu Asn Ile Glu Gly Asn Gly 
        35                  40                  45              


Gly Pro Gly Thr Ile Ala Lys Ile Ser Phe Pro Glu Gly Phe Pro Phe 
    50                  55                  60                  


Lys Tyr Val Lys Asp Arg Val Asp Glu Val Asp His Thr Asn Phe Lys 
65                  70                  75                  80  


Tyr Asn Tyr Ser Val Ile Glu Gly Gly Pro Ile Gly Asp Thr Leu Glu 
                85                  90                  95      


Lys Ile Ser Asn Glu Ile Lys Ile Val Ala Thr Pro Asp Gly Gly Ser 
            100                 105                 110         


Ile Leu Ala Ile Asn Asn Lys Tyr His Thr Ala Gly Asp His Glu Val 
        115                 120                 125             


Lys Ala Glu Gln Ile Lys Ala Ser Lys Glu Met Gly Glu Thr Leu Leu 
    130                 135                 140                 


Arg Ala Val Glu Ser Tyr Leu Leu Ala His Ser Asp Ala Tyr Asn 
145                 150                 155                 


<210>  7
<211>  477
<212>  DNA
<213>  Malus domestica

<400>  7
ggtgtctaca catttgagaa cgagttcacc tctgagattc caccatcaag attgttcaag       60

gcctttgtcc ttgatgctga caacctcatc cccaagattg caccccaggc aatcaagcaa      120

gctgaaatcc ttgaaggaga cggtggccct ggaaccatca aaaagatcac ttttggtgaa      180

ggtagccagt acggctacgt gaagcacaag atcgactcgg ttgacgaggc aaactactca      240

tacgcctaca ctttgattga aggagatgct ttgacagaca ccattgagaa ggtctcttac      300

gagaccaagt tggtggcatc tggaagtggt tccatcatca agagtatcag ccactaccac      360

accaagggtg atgttgagat caaggaagag cacgtcaagg ctggcaaaga gaaggctcat      420

ggtttgttca agcttattga gagctacctt aagggccacc ccgacgcata caactaa         477


<210>  8
<211>  480
<212>  DNA
<213>  betula verrucosa

<400>  8
ggtgttttca attacgaaac tgagaccacc tctgttatcc cagcagctcg actgttcaag       60

gcctttatcc ttgatggcga taatctcttt ccaaaggttg caccccaagc cattagcagt      120

gttgaaaaca ttgaaggaaa tggagggcct ggaaccatta agaagatcag ctttcccgaa      180

ggcttccctt tcaagtacgt gaaggacaga gttgatgagg tggaccacac aaacttcaaa      240

tacaattaca gcgtgatcga gggcggtccc ataggcgaca cattggagaa gatctccaac      300

gagataaaga tagtggcaac ccctgatgga ggttccatct tgaagatcaa caacaagtac      360

cataccaaag gagaccatga ggtgaaggca gagcagatta aggcaagtaa agaaatggga      420

gagacacttt tgagggccgt tgagagctac ctcttggcac actccgatgc ctacaactaa      480


<210>  9
<211>  960
<212>  DNA
<213>  Unknown

<220>
<223>  betula verrucosa - malus domestica hybrid

<400>  9
ggtgttttca attacgaaac tgagaccacc tctgttatcc cagcagctcg actgttcaag       60

gcctttatcc ttgatggcga taatctcttt ccaaaggttg caccccaagc cattagcagt      120

gttgaaaaca ttgaaggaaa tggagggcct ggaaccatta agaagatcag ctttcccgaa      180

ggcttccctt tcaagtacgt gaaggacaga gttgatgagg tggaccacac aaacttcaaa      240

tacaattaca gcgtgatcga gggcggtccc ataggcgaca cattggagaa gatctccaac      300

gagataaaga tagtggcaac ccctgatgga ggttccatct tgaagatcaa caacaagtac      360

cataccaaag gagaccatga ggtgaaggca gagcagatta aggcaagtaa agaaatggga      420

gagacacttt tgagggccgt tgagagctac ctcttggcac actccgatgc ctacaacgaa      480

ttcggtgtct acacatttga gaacgagttc acctctgaga ttccaccatc aagattgttc      540

aaggcctttg tccttgatgc tgacaacctc atccccaaga ttgcacccca ggcaatcaag      600

caagctgaaa tccttgaagg agacggtggc cctggaacca tcaaaaagat cacttttggt      660

gaaggtagcc agtacggcta cgtgaagcac aagatcgact cggttgacga ggcaaactac      720

tcatacgcct acactttgat tgaaggagat gctttgacag acaccattga gaaggtctct      780

tacgagacca agttggtggc atctggaagt ggttccatca tcaagagtat cagccactac      840

cacaccaagg gtgatgttga gatcaaggaa gagcacgtca aggctggcaa agagaaggct      900

catggtttgt tcaagcttat tgagagctac cttaagggcc accccgacgc atacaactaa      960


<210>  10
<211>  477
<212>  DNA
<213>  Unknown

<220>
<223>  malus domestica - mutant

<400>  10
ggtgtctaca catttgagaa cgagttcacc tctgagattc caccatcaag attgttcaag       60

gcctttgtcc ttgctgctga caacctcatc cccaagattg caccccaggc aatcaagcaa      120

gctgaaatcc ttgaaggaga cggtggccct ggaaccatca aaaagatcac ttttggtgaa      180

ggtagccagt acggctacgt gaagcacaag atcgactcgg ttgacgaggc agcctactca      240

tacgcctaca ctttgattga aggagatgct ttgacagaca ccattgagaa ggtctcttac      300

gagaccaagt tggtggcatc tggaagtggt tccatcatca agagtatcag ccactaccac      360

accaagggtg atgttgagat caaggaagag cacgtcaagg ctggcaaaga gaaggctcat      420

ggtttgttca agcttattga gagctacctt aagggccacc ccgacgcata caactaa         477


<210>  11
<211>  158
<212>  PRT
<213>  Unknown

<220>
<223>  malus domestica - mutant

<400>  11

Gly Val Tyr Thr Phe Glu Asn Glu Phe Thr Ser Glu Ile Pro Pro Ser 
1               5                   10                  15      


Arg Leu Phe Lys Ala Phe Val Leu Asp Ala Asp Asn Leu Ile Pro Lys 
            20                  25                  30          


Ile Ala Pro Gln Ala Ile Lys Gln Ala Glu Ile Leu Glu Gly Asp Gly 
        35                  40                  45              


Gly Pro Gly Thr Ile Lys Lys Ile Thr Phe Gly Glu Gly Ser Gln Tyr 
    50                  55                  60                  


Gly Tyr Val Lys His Lys Ile Asp Ser Val Asp Glu Ala Ala Tyr Ser 
65                  70                  75                  80  


Tyr Ala Tyr Thr Leu Ile Glu Gly Asp Ala Leu Thr Asp Thr Ile Glu 
                85                  90                  95      


Lys Val Ser Tyr Glu Thr Lys Leu Val Ala Ser Gly Ser Gly Ser Ile 
            100                 105                 110         


Ile Lys Ser Ile Ser His Tyr His Thr Lys Gly Asp Val Glu Ile Lys 
        115                 120                 125             


Glu Glu His Val Lys Ala Gly Lys Glu Lys Ala His Gly Leu Phe Lys 
    130                 135                 140                 


Leu Ile Glu Ser Tyr Leu Lys Gly His Pro Asp Ala Tyr Asn 
145                 150                 155             


<210>  12
<211>  27
<212>  DNA
<213>  Unknown

<220>
<223>  oligonucleotide

<400>  12
gcctttgtcc ttgctgctga caacctc                                           27


<210>  13
<211>  27
<212>  DNA
<213>  Unknown

<220>
<223>  oligonucleotide

<400>  13
gttgacgagg cagcctactc atacgcc                                           27


<210>  14
<211>  22
<212>  DNA
<213>  Unknown

<220>
<223>  oligonucleotide

<400>  14
ggtgttttca attacgaaac tg                                                22


<210>  15
<211>  24
<212>  DNA
<213>  Unknown

<220>
<223>  oligonucleotide

<400>  15
gcgaattcgt tgtaggcatc ggag                                              24


<210>  16
<211>  31
<212>  DNA
<213>  Unknown

<220>
<223>  oligonucleotide

<400>  16
cgcgaattcg gtgtctacac atttgagaac g                                      31


<210>  17
<211>  30
<212>  DNA
<213>  Unknown

<220>
<223>  oligonucleotide

<400>  17
gcgggatcct tagttgtatg cgtcggggtg                                        30


<210>  18
<211>  55
<212>  DNA
<213>  Unknown

<220>
<223>  oligonucleotide

<400>  18
gcgggtaccc atatgcatca ccatcaccat cacggtgttt tcaattacga aactg            55


<210>  19
<211>  960
<212>  DNA
<213>  Unknown

<220>
<223>  betula verrucosa - malus domestica hybrid mutant

<400>  19
ggtgttttca attacgaaac tgagaccacc tctgttatcc cagcagctcg actgttcaag       60

gcctttatcc ttgatggcga taatctcttt ccaaaggttg caccccaagc cattagcagt      120

gttgaaaaca ttgaaggaaa tggagggcct ggaaccattg cgaagatcag ctttcccgaa      180

ggcttccctt tcaagtacgt gaaggacaga gttgatgagg tggaccacac aaacttcaaa      240

tacaattaca gcgtgatcga gggcggtccc ataggcgaca cattggagaa gatctccaac      300

gagataaaga tagtggcaac ccctgatgga ggttccatct tggcgatcaa caacaagtac      360

cataccgcag gagaccatga ggtgaaggca gagcagatta aggcaagtaa agaaatggga      420

gagacacttt tgagggccgt tgagagctac ctcttggcac actccgatgc ctacaacgaa      480

ttcggtgtct acacatttga gaacgagttc acctctgaga ttccaccatc aagattgttc      540

aaggcctttg tccttgctgc tgacaacctc atccccaaga ttgcacccca ggcaatcaag      600

caagctgaaa tccttgaagg agacggtggc cctggaacca tcaaaaagat cacttttggt      660

gaaggtagcc agtacggcta cgtgaagcac aagatcgact cggttgacga ggcagcctac      720

tcatacgcct acactttgat tgaaggagat gctttgacag acaccattga gaaggtctct      780

tacgagacca agttggtggc atctggaagt ggttccatca tcaagagtat cagccactac      840

cacaccaagg gtgatgttga gatcaaggaa gagcacgtca aggctggcaa agagaaggct      900

catggtttgt tcaagcttat tgagagctac cttaagggcc accccgacgc atacaactaa      960


<210>  20
<211>  480
<212>  DNA
<213>  Unknown

<220>
<223>  betula verrucosa mutant

<400>  20
ggtgttttca attacgaaac tgagaccacc tctgttatcc cagcagctcg actgttcaag       60

gcctttatcc ttgatggcga taatctcttt ccaaaggttg caccccaagc cattagcagt      120

gttgaaaaca ttgaaggaaa tggagggcct ggaaccattg cgaagatcag ctttcccgaa      180

ggcttccctt tcaagtacgt gaaggacaga gttgatgagg tggaccacac aaacttcaaa      240

tacaattaca gcgtgatcga gggcggtccc ataggcgaca cattggagaa gatctccaac      300

gagataaaga tagtggcaac ccctgatgga ggttccatct tggcgatcaa caacaagtac      360

cataccgcag gagaccatga ggtgaaggca gagcagatta aggcaagtaa agaaatggga      420

gagacacttt tgagggccgt tgagagctac ctcttggcac actccgatgc ctacaactaa      480


