                         SEQUENCE LISTING

<110>  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
 
<120>  USE OF CELLULAR EXTRACTS FOR OBTAINING PLURIPOTENT STEM CELLS

<130>  IOB 11 CNR OCT4

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Oct4

<400>  1
ttctggcgcc ggttacagaa ccatactcga                                        30


<210>  2
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Oct4

<400>  2
gaggaagccg acaacaatga gaaccttcag                                        30


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Rex1

<400>  3
cagctcctgc acacagaaga                                                   20


<210>  4
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Rex1

<400>  4
actgatccgc aaacacctg                                                    19


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Nanog

<400>  5
ttcttgctta caagggtctg c                                                 21


<210>  6
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Nanog

<400>  6
agaggaaggg cgaggaga                                                     18


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Zfpm2

<400>  7
gcgaagacgt ggagttcttt                                                   20


<210>  8
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Zfpm2

<400>  8
ggctgtcccc atctgattc                                                    19


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from beta-actin

<400>  9
gccggcttac actgcgcttc tt                                                22


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from beta-actin

<400>  10
ttctggccca tgcccaccat                                                   20


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from GAPDH

<400>  11
tggcaaagtg gagattgttg c                                                 21


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from GAPDH

<400>  12
aagatggtga tgggcttccc g                                                 21


<210>  13
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Hprt1

<400>  13
tcctcctcag accgcttt                                                     18


<210>  14
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Hprt1

<400>  14
cctggttcat cgctaatc                                                     18


<210>  15
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Sox1

<400>  15
gtgacatctg cccccatc                                                     18


<210>  16
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Sox1

<400>  16
gaggccagtc tggtgtcag                                                    19


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Sox 17

<400>  17
ctttatggtg tgggccaaag                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Sox 17

<400>  18
ggtcaacgcc ttccaagact                                                   20


<210>  19
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Sox7

<400>  19
gcggagctca gcaagatg                                                     18


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Sox7

<400>  20
gggtctcttc tgggacagtg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Brachyury

<400>  21
cagcccacct actggctcta                                                   20


<210>  22
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Brachyury

<400>  22
gagcctgggg tgatggta                                                     18


<210>  23
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Klf4

<400>  23
gagttcctca cgccaacg                                                     18


<210>  24
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Klf4

<400>  24
cgggaaggga gaagacact                                                    19


<210>  25
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Oct4 for bisulfite 
       sequencing

<400>  25
ttagaggatg gttgagtggg tttgtaagga t                                      31


<210>  26
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Oct4 for bisulfite 
       sequencing

<400>  26
ccaatcccac cctctaacct taacctctaa                                        30


<210>  27
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Nanog for bisulfite 
       sequencing

<400>  27
taaattgggt atggtggtag ataagtttgg                                        30


<210>  28
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Nanog for bisulfite 
       sequencing

<400>  28
taaaaaacat cctctaatct aaaaacatcc                                        30


<210>  29
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Snrnp for bisulfite 
       sequencing

<400>  29
attggtgagt taattttttg ga                                                22


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Snrnp for bisulfite 
       sequencing

<400>  30
acaaaactcc tacatcctaa aa                                                22


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Oct4  for quantifying 
       integration

<400>  31
aagttggcgt ggagactttg                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Oct4 for quantifying 
       integration

<400>  32
tctgagttgc tttccactcg                                                   20


<210>  33
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Klf4  for quantifying 
       integration

<400>  33
gctcctctac agccgagaat c                                                 21


<210>  34
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Klf4 for quantifying 
       integration

<400>  34
atgtccgcca ggttgaag                                                     18


<210>  35
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from Sox2  for quantifying 
       integration

<400>  35
tcaagaggcc catgaacg                                                     18


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from Sox2  for quantifying 
       integration

<400>  36
ttgctgatct ccgagttgtg                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from cMyc  for quantifying 
       integration

<400>  37
gctggagatg atgaccgagt                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from cMyc  for quantifying 
       integration

<400>  38
atcgcagatg aagctctggt                                                   20


<210>  39
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from genomic region 1

<400>  39
gtcaccgttt gtgccgaa                                                     18


<210>  40
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from genomic region 1

<400>  40
agctgaaatg agaccgatta tgg                                               23


<210>  41
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotide derived from genomic region 2

<400>  41
gagtcaaaga gtggtgaagg agttagt                                           27


<210>  42
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotide derived from genomic region 2

<400>  42
agctgacggg ccttctaagt c                                                 21


