                         SEQUENCE LISTING

<110>  University of South Florida
       The United States Government as represented by the 
       Department of Veterans Affairs
 
<120>  Recombinant Adeno-Associated Virus-Mediated Expression of 
       Fractalkine for Treatment of Neuroinflammatory and 
       Neurodegenerative Diseases

<130>  USF.323XC1PCT

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence is synthesized

<400>  1
gagaccggtc caccatggct ccctcgccgc tcgcg                                  35


<210>  2
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence is synthesized

<400>  2
ctcgctagct cacatggcat agtcaggcac gtcataagga tagctagaag ccattgtggc       60

tgcctgggtg tcggggac                                                     78


<210>  3
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence is synthesized

<400>  3
gtagccccac tgcctgggca gctgtggctg cctgggtg                               38


<210>  4
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence is synthesized

<400>  4
ctcgctagct cacatggcat agtcaggcac gtcataagga tagctagaag ccatcactgg       60

caccaggacg tatgagttac                                                   80


<210>  5
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence is synthesized

<400>  5
cacccaggca gccacagctg cccaggcagt ggggctac                               38


<210>  6
<211>  395
<212>  PRT
<213>  Mus musculus

<400>  6

Met Ala Pro Ser Pro Leu Ala Trp Leu Leu Arg Leu Ala Ala Phe Phe 
1               5                   10                  15      


His Leu Cys Thr Leu Leu Pro Gly Gln His Leu Gly Met Thr Lys Cys 
            20                  25                  30          


Glu Ile Met Cys Asp Lys Met Thr Ser Arg Ile Pro Val Ala Leu Leu 
        35                  40                  45              


Ile Arg Tyr Gln Leu Asn Gln Glu Ser Cys Gly Lys Arg Ala Ile Val 
    50                  55                  60                  


Leu Glu Thr Thr Gln His Arg Arg Phe Cys Ala Asp Pro Lys Glu Lys 
65                  70                  75                  80  


Trp Val Gln Asp Ala Met Lys His Leu Asp His Gln Ala Ala Ala Leu 
                85                  90                  95      


Thr Lys Asn Gly Gly Lys Phe Glu Lys Arg Val Asp Asn Val Thr Pro 
            100                 105                 110         


Gly Ile Thr Leu Ala Thr Arg Gly Leu Ser Pro Ser Ala Leu Thr Lys 
        115                 120                 125             


Pro Glu Ser Ala Thr Leu Glu Asp Leu Ala Leu Glu Leu Thr Thr Ile 
    130                 135                 140                 


Ser Gln Glu Ala Arg Gly Thr Met Gly Thr Ser Gln Glu Pro Pro Ala 
145                 150                 155                 160 


Ala Val Thr Gly Ser Ser Leu Ser Thr Ser Glu Ala Gln Asp Ala Gly 
                165                 170                 175     


Leu Thr Ala Lys Pro Gln Ser Ile Gly Ser Phe Glu Ala Ala Asp Ile 
            180                 185                 190         


Ser Thr Thr Val Trp Pro Ser Pro Ala Val Tyr Gln Ser Gly Ser Ser 
        195                 200                 205             


Ser Trp Ala Glu Glu Lys Ala Thr Glu Ser Pro Ser Thr Thr Ala Pro 
    210                 215                 220                 


Ser Pro Gln Val Ser Thr Thr Ser Pro Ser Thr Pro Glu Glu Asn Val 
225                 230                 235                 240 


Gly Ser Glu Gly Gln Pro Pro Trp Val Gln Gly Gln Asp Leu Ser Pro 
                245                 250                 255     


Glu Lys Ser Leu Gly Ser Glu Glu Ile Asn Pro Val His Thr Asp Asn 
            260                 265                 270         


Phe Gln Glu Arg Gly Pro Gly Asn Thr Val His Pro Ser Val Ala Pro 
        275                 280                 285             


Ile Ser Ser Glu Glu Thr Pro Ser Pro Glu Leu Val Ala Ser Gly Ser 
    290                 295                 300                 


Gln Ala Pro Lys Ile Glu Glu Pro Ile His Ala Thr Ala Asp Pro Gln 
305                 310                 315                 320 


Lys Leu Ser Val Leu Ile Thr Pro Val Pro Asp Thr Gln Ala Ala Thr 
                325                 330                 335     


Arg Arg Gln Ala Val Gly Leu Leu Ala Phe Leu Gly Leu Leu Phe Cys 
            340                 345                 350         


Leu Gly Val Ala Met Phe Ala Tyr Gln Ser Leu Gln Gly Cys Pro Arg 
        355                 360                 365             


Lys Met Ala Gly Glu Met Val Glu Gly Leu Arg Tyr Val Pro Arg Ser 
    370                 375                 380                 


Cys Gly Ser Asn Ser Tyr Val Leu Val Pro Val 
385                 390                 395 


<210>  7
<211>  336
<212>  PRT
<213>  Mus musculus

<400>  7

Met Ala Pro Ser Pro Leu Ala Trp Leu Leu Arg Leu Ala Ala Phe Phe 
1               5                   10                  15      


His Leu Cys Thr Leu Leu Pro Gly Gln His Leu Gly Met Thr Lys Cys 
            20                  25                  30          


Glu Ile Met Cys Asp Lys Met Thr Ser Arg Ile Pro Val Ala Leu Leu 
        35                  40                  45              


Ile Arg Tyr Gln Leu Asn Gln Glu Ser Cys Gly Lys Arg Ala Ile Val 
    50                  55                  60                  


Leu Glu Thr Thr Gln His Arg Arg Phe Cys Ala Asp Pro Lys Glu Lys 
65                  70                  75                  80  


Trp Val Gln Asp Ala Met Lys His Leu Asp His Gln Ala Ala Ala Leu 
                85                  90                  95      


Thr Lys Asn Gly Gly Lys Phe Glu Lys Arg Val Asp Asn Val Thr Pro 
            100                 105                 110         


Gly Ile Thr Leu Ala Thr Arg Gly Leu Ser Pro Ser Ala Leu Thr Lys 
        115                 120                 125             


Pro Glu Ser Ala Thr Leu Glu Asp Leu Ala Leu Glu Leu Thr Thr Ile 
    130                 135                 140                 


Ser Gln Glu Ala Arg Gly Thr Met Gly Thr Ser Gln Glu Pro Pro Ala 
145                 150                 155                 160 


Ala Val Thr Gly Ser Ser Leu Ser Thr Ser Glu Ala Gln Asp Ala Gly 
                165                 170                 175     


Leu Thr Ala Lys Pro Gln Ser Ile Gly Ser Phe Glu Ala Ala Asp Ile 
            180                 185                 190         


Ser Thr Thr Val Trp Pro Ser Pro Ala Val Tyr Gln Ser Gly Ser Ser 
        195                 200                 205             


Ser Trp Ala Glu Glu Lys Ala Thr Glu Ser Pro Ser Thr Thr Ala Pro 
    210                 215                 220                 


Ser Pro Gln Val Ser Thr Thr Ser Pro Ser Thr Pro Glu Glu Asn Val 
225                 230                 235                 240 


Gly Ser Glu Gly Gln Pro Pro Trp Val Gln Gly Gln Asp Leu Ser Pro 
                245                 250                 255     


Glu Lys Ser Leu Gly Ser Glu Glu Ile Asn Pro Val His Thr Asp Asn 
            260                 265                 270         


Phe Gln Glu Arg Gly Pro Gly Asn Thr Val His Pro Ser Val Ala Pro 
        275                 280                 285             


Ile Ser Ser Glu Glu Thr Pro Ser Pro Glu Leu Val Ala Ser Gly Ser 
    290                 295                 300                 


Gln Ala Pro Lys Ile Glu Glu Pro Ile His Ala Thr Ala Asp Pro Gln 
305                 310                 315                 320 


Lys Leu Ser Val Leu Ile Thr Pro Val Pro Asp Thr Gln Ala Ala Thr 
                325                 330                 335     


<210>  8
<211>  395
<212>  PRT
<213>  Mus musculus

<400>  8

Met Ala Pro Ser Pro Leu Ala Trp Leu Leu Arg Leu Ala Ala Phe Phe 
1               5                   10                  15      


His Leu Cys Thr Leu Leu Pro Gly Gln His Leu Gly Met Thr Lys Cys 
            20                  25                  30          


Glu Ile Met Cys Asp Lys Met Thr Ser Arg Ile Pro Val Ala Leu Leu 
        35                  40                  45              


Ile Arg Tyr Gln Leu Asn Gln Glu Ser Cys Gly Lys Arg Ala Ile Val 
    50                  55                  60                  


Leu Glu Thr Thr Gln His Arg Arg Phe Cys Ala Asp Pro Lys Glu Lys 
65                  70                  75                  80  


Trp Val Gln Asp Ala Met Lys His Leu Asp His Gln Ala Ala Ala Leu 
                85                  90                  95      


Thr Lys Asn Gly Gly Lys Phe Glu Lys Arg Val Asp Asn Val Thr Pro 
            100                 105                 110         


Gly Ile Thr Leu Ala Thr Arg Gly Leu Ser Pro Ser Ala Leu Thr Lys 
        115                 120                 125             


Pro Glu Ser Ala Thr Leu Glu Asp Leu Ala Leu Glu Leu Thr Thr Ile 
    130                 135                 140                 


Ser Gln Glu Ala Arg Gly Thr Met Gly Thr Ser Gln Glu Pro Pro Ala 
145                 150                 155                 160 


Ala Val Thr Gly Ser Ser Leu Ser Thr Ser Glu Ala Gln Asp Ala Gly 
                165                 170                 175     


Leu Thr Ala Lys Pro Gln Ser Ile Gly Ser Phe Glu Ala Ala Asp Ile 
            180                 185                 190         


Ser Thr Thr Val Trp Pro Ser Pro Ala Val Tyr Gln Ser Gly Ser Ser 
        195                 200                 205             


Ser Trp Ala Glu Glu Lys Ala Thr Glu Ser Pro Ser Thr Thr Ala Pro 
    210                 215                 220                 


Ser Pro Gln Val Ser Thr Thr Ser Pro Ser Thr Pro Glu Glu Asn Val 
225                 230                 235                 240 


Gly Ser Glu Gly Gln Pro Pro Trp Val Gln Gly Gln Asp Leu Ser Pro 
                245                 250                 255     


Glu Lys Ser Leu Gly Ser Glu Glu Ile Asn Pro Val His Thr Asp Asn 
            260                 265                 270         


Phe Gln Glu Arg Gly Pro Gly Asn Thr Val His Pro Ser Val Ala Pro 
        275                 280                 285             


Ile Ser Ser Glu Glu Thr Pro Ser Pro Glu Leu Val Ala Ser Gly Ser 
    290                 295                 300                 


Gln Ala Pro Lys Ile Glu Glu Pro Ile His Ala Thr Ala Asp Pro Gln 
305                 310                 315                 320 


Lys Leu Ser Val Leu Ile Thr Pro Val Pro Asp Thr Gln Ala Ala Thr 
                325                 330                 335     


Ala Ala Gln Ala Val Gly Leu Leu Ala Phe Leu Gly Leu Leu Phe Cys 
            340                 345                 350         


Leu Gly Val Ala Met Phe Ala Tyr Gln Ser Leu Gln Gly Cys Pro Arg 
        355                 360                 365             


Lys Met Ala Gly Glu Met Val Glu Gly Leu Arg Tyr Val Pro Arg Ser 
    370                 375                 380                 


Cys Gly Ser Asn Ser Tyr Val Leu Val Pro Val 
385                 390                 395 


<210>  9
<211>  397
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Ala Pro Ile Ser Leu Ser Trp Leu Leu Arg Leu Ala Thr Phe Cys 
1               5                   10                  15      


His Leu Thr Val Leu Leu Ala Gly Gln His His Gly Val Thr Lys Cys 
            20                  25                  30          


Asn Ile Thr Cys Ser Lys Met Thr Ser Lys Ile Pro Val Ala Leu Leu 
        35                  40                  45              


Ile His Tyr Gln Gln Asn Gln Ala Ser Cys Gly Lys Arg Ala Ile Ile 
    50                  55                  60                  


Leu Glu Thr Arg Gln His Arg Leu Phe Cys Ala Asp Pro Lys Glu Gln 
65                  70                  75                  80  


Trp Val Lys Asp Ala Met Gln His Leu Asp Arg Gln Ala Ala Ala Leu 
                85                  90                  95      


Thr Arg Asn Gly Gly Thr Phe Glu Lys Gln Ile Gly Glu Val Lys Pro 
            100                 105                 110         


Arg Thr Thr Pro Ala Ala Gly Gly Met Asp Glu Ser Val Val Leu Glu 
        115                 120                 125             


Pro Glu Ala Thr Gly Glu Ser Ser Ser Leu Glu Pro Thr Pro Ser Ser 
    130                 135                 140                 


Gln Glu Ala Gln Arg Ala Leu Gly Thr Ser Pro Glu Leu Pro Thr Gly 
145                 150                 155                 160 


Val Thr Gly Ser Ser Gly Thr Arg Leu Pro Pro Thr Pro Lys Ala Gln 
                165                 170                 175     


Asp Gly Gly Pro Val Gly Thr Glu Leu Phe Arg Val Pro Pro Val Ser 
            180                 185                 190         


Thr Ala Ala Thr Trp Gln Ser Ser Ala Pro His Gln Pro Gly Pro Ser 
        195                 200                 205             


Leu Trp Ala Glu Ala Lys Thr Ser Glu Ala Pro Ser Thr Gln Asp Pro 
    210                 215                 220                 


Ser Thr Gln Ala Ser Thr Ala Ser Ser Pro Ala Pro Glu Glu Asn Ala 
225                 230                 235                 240 


Pro Ser Glu Gly Gln Arg Val Trp Gly Gln Gly Gln Ser Pro Arg Pro 
                245                 250                 255     


Glu Asn Ser Leu Glu Arg Glu Glu Met Gly Pro Val Pro Ala His Thr 
            260                 265                 270         


Asp Ala Phe Gln Asp Trp Gly Pro Gly Ser Met Ala His Val Ser Val 
        275                 280                 285             


Val Pro Val Ser Ser Glu Gly Thr Pro Ser Arg Glu Pro Val Ala Ser 
    290                 295                 300                 


Gly Ser Trp Thr Pro Lys Ala Glu Glu Pro Ile His Ala Thr Met Asp 
305                 310                 315                 320 


Pro Gln Arg Leu Gly Val Leu Ile Thr Pro Val Pro Asp Ala Gln Ala 
                325                 330                 335     


Ala Thr Arg Arg Gln Ala Val Gly Leu Leu Ala Phe Leu Gly Leu Leu 
            340                 345                 350         


Phe Cys Leu Gly Val Ala Met Phe Thr Tyr Gln Ser Leu Gln Gly Cys 
        355                 360                 365             


Pro Arg Lys Met Ala Gly Glu Met Ala Glu Gly Leu Arg Tyr Ile Pro 
    370                 375                 380                 


Arg Ser Cys Gly Ser Asn Ser Tyr Val Leu Val Pro Val 
385                 390                 395         


<210>  10
<211>  341
<212>  PRT
<213>  Homo sapiens

<400>  10

Met Ala Pro Ile Ser Leu Ser Trp Leu Leu Arg Leu Ala Thr Phe Cys 
1               5                   10                  15      


His Leu Thr Val Leu Leu Ala Gly Gln His His Gly Val Thr Lys Cys 
            20                  25                  30          


Asn Ile Thr Cys Ser Lys Met Thr Ser Lys Ile Pro Val Ala Leu Leu 
        35                  40                  45              


Ile His Tyr Gln Gln Asn Gln Ala Ser Cys Gly Lys Arg Ala Ile Ile 
    50                  55                  60                  


Leu Glu Thr Arg Gln His Arg Leu Phe Cys Ala Asp Pro Lys Glu Gln 
65                  70                  75                  80  


Trp Val Lys Asp Ala Met Gln His Leu Asp Arg Gln Ala Ala Ala Leu 
                85                  90                  95      


Thr Arg Asn Gly Gly Thr Phe Glu Lys Gln Ile Gly Glu Val Lys Pro 
            100                 105                 110         


Arg Thr Thr Pro Ala Ala Gly Gly Met Asp Glu Ser Val Val Leu Glu 
        115                 120                 125             


Pro Glu Ala Thr Gly Glu Ser Ser Ser Leu Glu Pro Thr Pro Ser Ser 
    130                 135                 140                 


Gln Glu Ala Gln Arg Ala Leu Gly Thr Ser Pro Glu Leu Pro Thr Gly 
145                 150                 155                 160 


Val Thr Gly Ser Ser Gly Thr Arg Leu Pro Pro Thr Pro Lys Ala Gln 
                165                 170                 175     


Asp Gly Gly Pro Val Gly Thr Glu Leu Phe Arg Val Pro Pro Val Ser 
            180                 185                 190         


Thr Ala Ala Thr Trp Gln Ser Ser Ala Pro His Gln Pro Gly Pro Ser 
        195                 200                 205             


Leu Trp Ala Glu Ala Lys Thr Ser Glu Ala Pro Ser Thr Gln Asp Pro 
    210                 215                 220                 


Ser Thr Gln Ala Ser Thr Ala Ser Ser Pro Ala Pro Glu Glu Asn Ala 
225                 230                 235                 240 


Pro Ser Glu Gly Gln Arg Val Trp Gly Gln Gly Gln Ser Pro Arg Pro 
                245                 250                 255     


Glu Asn Ser Leu Glu Arg Glu Glu Met Gly Pro Val Pro Ala His Thr 
            260                 265                 270         


Asp Ala Phe Gln Asp Trp Gly Pro Gly Ser Met Ala His Val Ser Val 
        275                 280                 285             


Val Pro Val Ser Ser Glu Gly Thr Pro Ser Arg Glu Pro Val Ala Ser 
    290                 295                 300                 


Gly Ser Trp Thr Pro Lys Ala Glu Glu Pro Ile His Ala Thr Met Asp 
305                 310                 315                 320 


Pro Gln Arg Leu Gly Val Leu Ile Thr Pro Val Pro Asp Ala Gln Ala 
                325                 330                 335     


Ala Thr Arg Arg Gln 
            340     


<210>  11
<211>  397
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Ala Pro Ile Ser Leu Ser Trp Leu Leu Arg Leu Ala Thr Phe Cys 
1               5                   10                  15      


His Leu Thr Val Leu Leu Ala Gly Gln His His Gly Val Thr Lys Cys 
            20                  25                  30          


Asn Ile Thr Cys Ser Lys Met Thr Ser Lys Ile Pro Val Ala Leu Leu 
        35                  40                  45              


Ile His Tyr Gln Gln Asn Gln Ala Ser Cys Gly Lys Arg Ala Ile Ile 
    50                  55                  60                  


Leu Glu Thr Arg Gln His Arg Leu Phe Cys Ala Asp Pro Lys Glu Gln 
65                  70                  75                  80  


Trp Val Lys Asp Ala Met Gln His Leu Asp Arg Gln Ala Ala Ala Leu 
                85                  90                  95      


Thr Arg Asn Gly Gly Thr Phe Glu Lys Gln Ile Gly Glu Val Lys Pro 
            100                 105                 110         


Arg Thr Thr Pro Ala Ala Gly Gly Met Asp Glu Ser Val Val Leu Glu 
        115                 120                 125             


Pro Glu Ala Thr Gly Glu Ser Ser Ser Leu Glu Pro Thr Pro Ser Ser 
    130                 135                 140                 


Gln Glu Ala Gln Arg Ala Leu Gly Thr Ser Pro Glu Leu Pro Thr Gly 
145                 150                 155                 160 


Val Thr Gly Ser Ser Gly Thr Arg Leu Pro Pro Thr Pro Lys Ala Gln 
                165                 170                 175     


Asp Gly Gly Pro Val Gly Thr Glu Leu Phe Arg Val Pro Pro Val Ser 
            180                 185                 190         


Thr Ala Ala Thr Trp Gln Ser Ser Ala Pro His Gln Pro Gly Pro Ser 
        195                 200                 205             


Leu Trp Ala Glu Ala Lys Thr Ser Glu Ala Pro Ser Thr Gln Asp Pro 
    210                 215                 220                 


Ser Thr Gln Ala Ser Thr Ala Ser Ser Pro Ala Pro Glu Glu Asn Ala 
225                 230                 235                 240 


Pro Ser Glu Gly Gln Arg Val Trp Gly Gln Gly Gln Ser Pro Arg Pro 
                245                 250                 255     


Glu Asn Ser Leu Glu Arg Glu Glu Met Gly Pro Val Pro Ala His Thr 
            260                 265                 270         


Asp Ala Phe Gln Asp Trp Gly Pro Gly Ser Met Ala His Val Ser Val 
        275                 280                 285             


Val Pro Val Ser Ser Glu Gly Thr Pro Ser Arg Glu Pro Val Ala Ser 
    290                 295                 300                 


Gly Ser Trp Thr Pro Lys Ala Glu Glu Pro Ile His Ala Thr Met Asp 
305                 310                 315                 320 


Pro Gln Arg Leu Gly Val Leu Ile Thr Pro Val Pro Asp Ala Gln Ala 
                325                 330                 335     


Ala Thr Ala Ala Gln Ala Val Gly Leu Leu Ala Phe Leu Gly Leu Leu 
            340                 345                 350         


Phe Cys Leu Gly Val Ala Met Phe Thr Tyr Gln Ser Leu Gln Gly Cys 
        355                 360                 365             


Pro Arg Lys Met Ala Gly Glu Met Ala Glu Gly Leu Arg Tyr Ile Pro 
    370                 375                 380                 


Arg Ser Cys Gly Ser Asn Ser Tyr Val Leu Val Pro Val 
385                 390                 395         


