                         SEQUENCE LISTING

<110>  Waterford Institute of Technology
 
<120>  A Nitrile-Metabolising Enzyme and a Process for Producing an Acid
        using Same

<130>  P120817.WO.01

<150>  GB1111637.3
<151>  2011-07-07

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  374
<212>  DNA
<213>  Pantoea sp. SS-4

<400>  1
gctggcgcgc tacagcctga tgacgcagca cgaggagatc cactgcagcc agttccccgg       60

ctcgctggtg gggccgattt tcgcggagca gatggatgtc accatccgtc atcatgcgct      120

ggagtccggc tgttttgtca tcaacgccac cggctggctg acggaaacgc aaatcaatga      180

attaacagcc gatcccgcgt tgcagaaagg gctgcgcggc ggatgcaaca ccgcgattat      240

ttcccctgaa ggccgccatc tggtgccgcc gctgaccgaa ggggaaggga tcctggtggc      300

cgatctggat atggcgctga tcaccaagcg taagcggatg atggattccg tgggccacta      360

cgcgcgcgcg gaaa                                                        374


<210>  2
<211>  124
<212>  PRT
<213>  Pantoea sp. SS-4

<400>  2

Leu Ala Arg Tyr Ser Leu Met Thr Gln His Glu Glu Ile His Cys Ser 
1               5                   10                  15      


Gln Phe Pro Gly Ser Leu Val Gly Pro Ile Phe Ala Glu Gln Met Asp 
            20                  25                  30          


Val Thr Ile Arg His His Ala Leu Glu Ser Gly Cys Phe Val Ile Asn 
        35                  40                  45              


Ala Thr Gly Trp Leu Thr Glu Thr Gln Ile Asn Glu Leu Thr Ala Asp 
    50                  55                  60                  


Pro Ala Leu Gln Lys Gly Leu Arg Gly Gly Cys Asn Thr Ala Ile Ile 
65                  70                  75                  80  


Ser Pro Glu Gly Arg His Leu Val Pro Pro Leu Thr Glu Gly Glu Gly 
                85                  90                  95      


Ile Leu Val Ala Asp Leu Asp Met Ala Leu Ile Thr Lys Arg Lys Arg 
            100                 105                 110         


Met Met Asp Ser Val Gly His Tyr Ala Arg Ala Glu 
        115                 120                 


<210>  3
<211>  1227
<212>  DNA
<213>  Pantoea sp. SS-4

<400>  3
aatgtctggg aaactgcccg atggaggggg ataactactg gaaacggtag ctaataccgc       60

ataacgtctt cggaccaaag tgggggacct tcgggcctca caccatcgga tgtgcccaga      120

tgggattagc tagtaggtgg ggtaatggct cacctaggcg acgatcccta gctggtctga      180

gaggatgacc agccacactg gaactgagac acggtccaga ctcctacggg aggcagcagt      240

ggggaatatt gcacaatggg cgcaagcctg atgcagccat gccgcgtgta tgaagaaggc      300

cttcgggttg taaagtactt tcagcgggga ggaaggcgat gaggttaata accttatcga      360

ttgacgttac ccgcagaaga agcaccggct aactccgtgc cagcagccgc ggtaatacgg      420

agggtgcaag cgttaatcgg aattactggg cgtaaagcgc acgcaggcgg tctgtcaagt      480

cagatgtgaa atccccgggc ttaacctggg aactgcattt gaaactggca ggcttgagtc      540

tcgtagaggg gggtagaatt ccaggtgtag cggtgaaatg cgtagagatc tggaggaata      600

ccggtggcga aggcggcccc ctggacgaag actgacgctc aggtgcgaaa gcgtggggag      660

caaacaggat tagataccct ggtagtccac gctgtaaacg atgtcgactt ggaggttgtg      720

cccttgaggc gtggcttccg gagctaacgc gttaagtcga ccgcctgggg agtacggccg      780

caaggttaaa actcaaatga attgacgggg gcccgcacaa gcggtggagc atgtggttta      840

attcgatgca acgcgaagaa ccttacctgg ccttgacatc cacagaactt tccagagatg      900

gattggtgcc ttcgggaact gtgagacagg tgctgcatgg ctgtcgtcag ctcgtgttgt      960

gaaatgttgg gttaagtccc gcaacgagcg caacccttat cctttgttgc cagcgattcg     1020

gtcgggaact caaaggagac tgccggtgat aaaccggagg aaggtgggga tgacgtcaag     1080

tcatcatggc ccttacggcc agggctacac acgtgctaca atggcgcata caaagagaag     1140

cgacctcgcg agagcaagcg gacctcataa agtgcgtcgt agtccggatc ggagtctgca     1200

actcgactcc gtgaagtcgg aatcgct                                         1227


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1B F primer

<400>  4
tgytgggarc aytacaaycc                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1B R primer

<400>  5
tccatcatbc kyttkcgytt                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2A F primer

<400>  6
gsvytbtgct gytgggarca                                                   20


<210>  7
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2A R1 primer

<400>  7
rtartgvccr gcvggrtc                                                     18


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2A R2 primer

<400>  8
gartartgsc cgrcsggrtc                                                   20


