                         SEQUENCE LISTING

<110>  GENZYME CORPORATION
       ZHU, Yunxiang
       KARMAN, Jozsef
       WEI, Ronnie
       JIANG, Canwen
       CHEN, Seng
 
<120>  INHIBITORS OF T-CELL ACTIVATION

<130>  20868WO(300232)

<140>  PCT/US2012/045017
<141>  2012-06-29

<150>  US 61/503,282
<151>  2011-06-30

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  2160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of mouse surrogate construct (Gene1)

<400>  1
atggcttgca attgtcagtt gatgcaggat acaccactcc tcaagtttcc atgtccaagg       60

ctcattcttc tctttgtgct gctgattcgt ctttcacaag tgtcttcaga tgttgatgaa      120

caactgtcca agtcagtgaa agataaggta ttgctgcctt gccgttacaa ctctcctcat      180

gaagatgagt ctgaagaccg aatctactgg caaaaacatg acaaagtggt gctgtctgtc      240

attgctggga aactaaaagt ggcgcccgag tataagaacc ggactttata tgacaacact      300

acctactctc ttatcatcct gggcctggtc ctttcagacc ggggcacata cagctgtgtc      360

gttcaaaaga aggaaagagg aacgtatgaa gttaaacact tggctttagt aaagttgtcc      420

atcaaagctg acttctctac ccccaacata actgagtctg gaaacccatc tgcagacact      480

aaaaggatta cctgctttgc ttccgggggt ttcccaaagc ctcgcttctc ttggttggaa      540

aatggaagag aattacctgg catcaatacg acaatttccc aggatcctga atctgaattg      600

tacaccatta gtagccaact agatttcaat acgactcgca accacaccat taagtgtctc      660

attaaatatg gagatgctca cgtgtcagag gacttcacct gggagcccag agggcccaca      720

atcaagccct gtcctccatg caaatgccca gcacctaacc tcttgggtgg accatccgtc      780

ttcatcttcc ctccaaagat caaggatgta ctcatgatct ccctgagccc catggtcaca      840

tgtgtggtgg tggatgtgag cgaggatgac ccagatgtcc agatcagctg gttcgtgaac      900

aacgtggaag tactcacagc tcagacacaa acccatagag aggattacaa cagtactctc      960

cgggtggtca gtgccctccc catccagcac caggactgga tgagtggcaa ggagttcaaa     1020

tgcaaggtca acaacaaagc cctcccagcg cccatcgaga gaaccatctc aaaacccaaa     1080

gggtcagtaa gagctccaca ggtatatgtc ttgcctccac cagaagaaga gatgactaag     1140

aaacaggtca ctctgacctg catggtcaca gacttcatgc ctgaagacat ttacgtggag     1200

tggaccaaca acgggaaaac agagctaaac tacaagaaca ctgaaccagt cctggactct     1260

gatggttctt acttcatgta cagcaagctg agagtggaaa agaagaactg ggtggaaaga     1320

aatagctact cctgctcagt ggtccacgag ggtctgcaca atcaccacac gactaagagc     1380

ttctcccgga ctccgggtaa aggcggtggc ggcggaggcg gtggcggtgg gcctgggaaa     1440

gagctccccg tggtgtgggc ccaggaggga gctcccgtcc atcttccctg cagcctcaaa     1500

tcccccaacc tggatcctaa ctttctacga agaggagggg ttatctggca acatcaacca     1560

gacagtggcc aacccactcc catcccggcc cttgaccttc accaggggat gccctcgcct     1620

agacaacccg cacccggtcg ctacacggtg ctgagcgtgg ctccaggagg cctgcgcagc     1680

gggaggcagc ccctgcatcc ccacgtgcag ctggaggagc gcggcctcca gcgcggggac     1740

ttctctctgt ggttgcgccc agctctgcgc accgatgcgg gcgagtacca cgccaccgtg     1800

cgcctcccga accgcgccct ctcctgcagt ctccgcctgc gcgtcggcca ggcctcgatg     1860

attgctagtc cctcaggagt cctcaagctg tctgattggg tccttttgaa ctgctccttc     1920

agccgtcctg accgcccagt ctctgtgcac tggttccagg gccagaaccg agtgcctgtc     1980

tacaactcac cgcgtcattt tttagctgaa actttcctgt tactgcccca agtcagcccc     2040

ctggactctg ggacctgggg ctgtgtcctc acctacagag atggcttcaa tgtctccatc     2100

acgtacaacc tcaaggttct gggtctggag cccgtagccc accatcacca tcatcactga     2160


<210>  2
<211>  719
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Translated protein sequence of mouse surrogate construct (Gene1)

<400>  2

Met Ala Cys Asn Cys Gln Leu Met Gln Asp Thr Pro Leu Leu Lys Phe 
1               5                   10                  15      


Pro Cys Pro Arg Leu Ile Leu Leu Phe Val Leu Leu Ile Arg Leu Ser 
            20                  25                  30          


Gln Val Ser Ser Asp Val Asp Glu Gln Leu Ser Lys Ser Val Lys Asp 
        35                  40                  45              


Lys Val Leu Leu Pro Cys Arg Tyr Asn Ser Pro His Glu Asp Glu Ser 
    50                  55                  60                  


Glu Asp Arg Ile Tyr Trp Gln Lys His Asp Lys Val Val Leu Ser Val 
65                  70                  75                  80  


Ile Ala Gly Lys Leu Lys Val Ala Pro Glu Tyr Lys Asn Arg Thr Leu 
                85                  90                  95      


Tyr Asp Asn Thr Thr Tyr Ser Leu Ile Ile Leu Gly Leu Val Leu Ser 
            100                 105                 110         


Asp Arg Gly Thr Tyr Ser Cys Val Val Gln Lys Lys Glu Arg Gly Thr 
        115                 120                 125             


Tyr Glu Val Lys His Leu Ala Leu Val Lys Leu Ser Ile Lys Ala Asp 
    130                 135                 140                 


Phe Ser Thr Pro Asn Ile Thr Glu Ser Gly Asn Pro Ser Ala Asp Thr 
145                 150                 155                 160 


Lys Arg Ile Thr Cys Phe Ala Ser Gly Gly Phe Pro Lys Pro Arg Phe 
                165                 170                 175     


Ser Trp Leu Glu Asn Gly Arg Glu Leu Pro Gly Ile Asn Thr Thr Ile 
            180                 185                 190         


Ser Gln Asp Pro Glu Ser Glu Leu Tyr Thr Ile Ser Ser Gln Leu Asp 
        195                 200                 205             


Phe Asn Thr Thr Arg Asn His Thr Ile Lys Cys Leu Ile Lys Tyr Gly 
    210                 215                 220                 


Asp Ala His Val Ser Glu Asp Phe Thr Trp Glu Pro Arg Gly Pro Thr 
225                 230                 235                 240 


Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly 
                245                 250                 255     


Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met 
            260                 265                 270         


Ile Ser Leu Ser Pro Met Val Thr Cys Val Val Val Asp Val Ser Glu 
        275                 280                 285             


Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val 
    290                 295                 300                 


Leu Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu 
305                 310                 315                 320 


Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly 
                325                 330                 335     


Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Ala Leu Pro Ala Pro Ile 
            340                 345                 350         


Glu Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val 
        355                 360                 365             


Tyr Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr 
    370                 375                 380                 


Leu Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu 
385                 390                 395                 400 


Trp Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro 
                405                 410                 415     


Val Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val 
            420                 425                 430         


Glu Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val 
        435                 440                 445             


His Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr 
    450                 455                 460                 


Pro Gly Lys Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Pro Gly Lys 
465                 470                 475                 480 


Glu Leu Pro Val Val Trp Ala Gln Glu Gly Ala Pro Val His Leu Pro 
                485                 490                 495     


Cys Ser Leu Lys Ser Pro Asn Leu Asp Pro Asn Phe Leu Arg Arg Gly 
            500                 505                 510         


Gly Val Ile Trp Gln His Gln Pro Asp Ser Gly Gln Pro Thr Pro Ile 
        515                 520                 525             


Pro Ala Leu Asp Leu His Gln Gly Met Pro Ser Pro Arg Gln Pro Ala 
    530                 535                 540                 


Pro Gly Arg Tyr Thr Val Leu Ser Val Ala Pro Gly Gly Leu Arg Ser 
545                 550                 555                 560 


Gly Arg Gln Pro Leu His Pro His Val Gln Leu Glu Glu Arg Gly Leu 
                565                 570                 575     


Gln Arg Gly Asp Phe Ser Leu Trp Leu Arg Pro Ala Leu Arg Thr Asp 
            580                 585                 590         


Ala Gly Glu Tyr His Ala Thr Val Arg Leu Pro Asn Arg Ala Leu Ser 
        595                 600                 605             


Cys Ser Leu Arg Leu Arg Val Gly Gln Ala Ser Met Ile Ala Ser Pro 
    610                 615                 620                 


Ser Gly Val Leu Lys Leu Ser Asp Trp Val Leu Leu Asn Cys Ser Phe 
625                 630                 635                 640 


Ser Arg Pro Asp Arg Pro Val Ser Val His Trp Phe Gln Gly Gln Asn 
                645                 650                 655     


Arg Val Pro Val Tyr Asn Ser Pro Arg His Phe Leu Ala Glu Thr Phe 
            660                 665                 670         


Leu Leu Leu Pro Gln Val Ser Pro Leu Asp Ser Gly Thr Trp Gly Cys 
        675                 680                 685             


Val Leu Thr Tyr Arg Asp Gly Phe Asn Val Ser Ile Thr Tyr Asn Leu 
    690                 695                 700                 


Lys Val Leu Gly Leu Glu Pro Val Ala His His His His His His 
705                 710                 715                 


<210>  3
<211>  2139
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of mouse surrogate construct (Gene 2)

<400>  3
atggcttgca attgtcagtt gatgcaggat acaccactcc tcaagtttcc atgtccaagg       60

ctcattcttc tctttgtgct gctgattcgt ctttcacaag tgtcttcaga tgttgatgaa      120

caactgtcca agtcagtgaa agataaggta ttgctgcctt gccgttacaa ctctcctcat      180

gaagatgagt ctgaagaccg aatctactgg caaaaacatg acaaagtggt gctgtctgtc      240

attgctggga aactaaaagt ggcgcccgag tataagaacc ggactttata tgacaacact      300

acctactctc ttatcatcct gggcctggtc ctttcagacc ggggcacata cagctgtgtc      360

gttcaaaaga aggaaagagg aacgtatgaa gttaaacact tggctttagt aaagttgtcc      420

atcaaagctg acttctctac ccccaacata actgagtctg gaaacccatc tgcagacact      480

aaaaggatta cctgctttgc ttccgggggt ttcccaaagc ctcgcttctc ttggttggaa      540

aatggaagag aattacctgg catcaatacg acaatttccc aggatcctga atctgaattg      600

tacaccatta gtagccaact agatttcaat acgactcgca accacaccat taagtgtctc      660

attaaatatg gagatgctca cgtgtcagag gacttcacct ggggcggtgg cggcggaggc      720

ggtggcggtg ggcctgggaa agagctcccc gtggtgtggg cccaggaggg agctcccgtc      780

catcttccct gcagcctcaa atcccccaac ctggatccta actttctacg aagaggaggg      840

gttatctggc aacatcaacc agacagtggc caacccactc ccatcccggc ccttgacctt      900

caccagggga tgccctcgcc tagacaaccc gcacccggtc gctacacggt gctgagcgtg      960

gctccaggag gcctgcgcag cgggaggcag cccctgcatc cccacgtgca gctggaggag     1020

cgcggcctcc agcgcgggga cttctctctg tggttgcgcc cagctctgcg caccgatgcg     1080

ggcgagtacc acgccaccgt gcgcctcccg aaccgcgccc tctcctgcag tctccgcctg     1140

cgcgtcggcc aggcctcgat gattgctagt ccctcaggag tcctcaagct gtctgattgg     1200

gtccttttga actgctcctt cagccgtcct gaccgcccag tctctgtgca ctggttccag     1260

ggccagaacc gagtgcctgt ctacaactca ccgcgtcatt ttttagctga aactttcctg     1320

ttactgcccc aagtcagccc cctggactct gggacctggg gctgtgtcct cacctacaga     1380

gatggcttca atgtctccat cacgtacaac ctcaaggttc tgggtctgga gcccgtagcc     1440

cccagagggc ccacaatcaa gccctgtcct ccatgcaaat gcccagcacc taacctcttg     1500

ggtggaccat ccgtcttcat cttccctcca aagatcaagg atgtactcat gatctccctg     1560

agccccatgg tcacatgtgt ggtggtggat gtgagcgagg atgacccaga tgtccagatc     1620

agctggttcg tgaacaacgt ggaagtactc acagctcaga cacaaaccca tagagaggat     1680

tacaacagta ctctccgggt ggtcagtgcc ctccccatcc agcaccagga ctggatgagt     1740

ggcaaggagt tcaaatgcaa ggtcaacaac aaagccctcc cagcgcccat cgagagaacc     1800

atctcaaaac ccaaagggtc agtaagagct ccacaggtat atgtcttgcc tccaccagaa     1860

gaagagatga ctaagaaaca ggtcactctg acctgcatgg tcacagactt catgcctgaa     1920

gacatttacg tggagtggac caacaacggg aaaacagagc taaactacaa gaacactgaa     1980

ccagtcctgg actctgatgg ttcttacttc atgtacagca agctgagagt ggaaaagaag     2040

aactgggtgg aaagaaatag ctactcctgc tcagtggtcc acgagggtct gcacaatcac     2100

cacacgacta agagcttctc ccggactccg ggtaaatga                            2139


<210>  4
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Translated protein sequence of mouse surrogate construct (Gene 2)

<400>  4

Met Ala Cys Asn Cys Gln Leu Met Gln Asp Thr Pro Leu Leu Lys Phe 
1               5                   10                  15      


Pro Cys Pro Arg Leu Ile Leu Leu Phe Val Leu Leu Ile Arg Leu Ser 
            20                  25                  30          


Gln Val Ser Ser Asp Val Asp Glu Gln Leu Ser Lys Ser Val Lys Asp 
        35                  40                  45              


Lys Val Leu Leu Pro Cys Arg Tyr Asn Ser Pro His Glu Asp Glu Ser 
    50                  55                  60                  


Glu Asp Arg Ile Tyr Trp Gln Lys His Asp Lys Val Val Leu Ser Val 
65                  70                  75                  80  


Ile Ala Gly Lys Leu Lys Val Ala Pro Glu Tyr Lys Asn Arg Thr Leu 
                85                  90                  95      


Tyr Asp Asn Thr Thr Tyr Ser Leu Ile Ile Leu Gly Leu Val Leu Ser 
            100                 105                 110         


Asp Arg Gly Thr Tyr Ser Cys Val Val Gln Lys Lys Glu Arg Gly Thr 
        115                 120                 125             


Tyr Glu Val Lys His Leu Ala Leu Val Lys Leu Ser Ile Lys Ala Asp 
    130                 135                 140                 


Phe Ser Thr Pro Asn Ile Thr Glu Ser Gly Asn Pro Ser Ala Asp Thr 
145                 150                 155                 160 


Lys Arg Ile Thr Cys Phe Ala Ser Gly Gly Phe Pro Lys Pro Arg Phe 
                165                 170                 175     


Ser Trp Leu Glu Asn Gly Arg Glu Leu Pro Gly Ile Asn Thr Thr Ile 
            180                 185                 190         


Ser Gln Asp Pro Glu Ser Glu Leu Tyr Thr Ile Ser Ser Gln Leu Asp 
        195                 200                 205             


Phe Asn Thr Thr Arg Asn His Thr Ile Lys Cys Leu Ile Lys Tyr Gly 
    210                 215                 220                 


Asp Ala His Val Ser Glu Asp Phe Thr Trp Gly Gly Gly Gly Gly Gly 
225                 230                 235                 240 


Gly Gly Gly Gly Pro Gly Lys Glu Leu Pro Val Val Trp Ala Gln Glu 
                245                 250                 255     


Gly Ala Pro Val His Leu Pro Cys Ser Leu Lys Ser Pro Asn Leu Asp 
            260                 265                 270         


Pro Asn Phe Leu Arg Arg Gly Gly Val Ile Trp Gln His Gln Pro Asp 
        275                 280                 285             


Ser Gly Gln Pro Thr Pro Ile Pro Ala Leu Asp Leu His Gln Gly Met 
    290                 295                 300                 


Pro Ser Pro Arg Gln Pro Ala Pro Gly Arg Tyr Thr Val Leu Ser Val 
305                 310                 315                 320 


Ala Pro Gly Gly Leu Arg Ser Gly Arg Gln Pro Leu His Pro His Val 
                325                 330                 335     


Gln Leu Glu Glu Arg Gly Leu Gln Arg Gly Asp Phe Ser Leu Trp Leu 
            340                 345                 350         


Arg Pro Ala Leu Arg Thr Asp Ala Gly Glu Tyr His Ala Thr Val Arg 
        355                 360                 365             


Leu Pro Asn Arg Ala Leu Ser Cys Ser Leu Arg Leu Arg Val Gly Gln 
    370                 375                 380                 


Ala Ser Met Ile Ala Ser Pro Ser Gly Val Leu Lys Leu Ser Asp Trp 
385                 390                 395                 400 


Val Leu Leu Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Val Ser Val 
                405                 410                 415     


His Trp Phe Gln Gly Gln Asn Arg Val Pro Val Tyr Asn Ser Pro Arg 
            420                 425                 430         


His Phe Leu Ala Glu Thr Phe Leu Leu Leu Pro Gln Val Ser Pro Leu 
        435                 440                 445             


Asp Ser Gly Thr Trp Gly Cys Val Leu Thr Tyr Arg Asp Gly Phe Asn 
    450                 455                 460                 


Val Ser Ile Thr Tyr Asn Leu Lys Val Leu Gly Leu Glu Pro Val Ala 
465                 470                 475                 480 


Pro Arg Gly Pro Thr Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala 
                485                 490                 495     


Pro Asn Leu Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Ile 
            500                 505                 510         


Lys Asp Val Leu Met Ile Ser Leu Ser Pro Met Val Thr Cys Val Val 
        515                 520                 525             


Val Asp Val Ser Glu Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val 
    530                 535                 540                 


Asn Asn Val Glu Val Leu Thr Ala Gln Thr Gln Thr His Arg Glu Asp 
545                 550                 555                 560 


Tyr Asn Ser Thr Leu Arg Val Val Ser Ala Leu Pro Ile Gln His Gln 
                565                 570                 575     


Asp Trp Met Ser Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Ala 
            580                 585                 590         


Leu Pro Ala Pro Ile Glu Arg Thr Ile Ser Lys Pro Lys Gly Ser Val 
        595                 600                 605             


Arg Ala Pro Gln Val Tyr Val Leu Pro Pro Pro Glu Glu Glu Met Thr 
    610                 615                 620                 


Lys Lys Gln Val Thr Leu Thr Cys Met Val Thr Asp Phe Met Pro Glu 
625                 630                 635                 640 


Asp Ile Tyr Val Glu Trp Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr 
                645                 650                 655     


Lys Asn Thr Glu Pro Val Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr 
            660                 665                 670         


Ser Lys Leu Arg Val Glu Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr 
        675                 680                 685             


Ser Cys Ser Val Val His Glu Gly Leu His Asn His His Thr Thr Lys 
    690                 695                 700                 


Ser Phe Ser Arg Thr Pro Gly Lys 
705                 710         


<210>  5
<211>  2160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTLA-4 BsB human construct wildtype nucleotide sequence

<400>  5
atgggccaca cacggaggca gggaacatca ccatccaagt gtccatacct caatttcttt       60

cagctcttgg tgctggctgg tctttctcac ttctgttcag gtgttatcca cgtgaccaag      120

gaagtgaaag aagtggcaac gctgtcctgt ggtcacaatg tttctgttga agagctggca      180

caaactcgca tctactggca aaaggagaag aaaatggtgc tgactatgat gtctggggac      240

atgaatatat ggcccgagta caagaaccgg accatctttg atatcactaa taacctctcc      300

attgtgatcc tggctctgcg cccatctgac gagggcacat acgagtgtgt tgttctgaag      360

tatgaaaaag acgctttcaa gcgggaacac ctggctgaag tgacgttatc agtcaaagct      420

gacttcccta cacctagtat atctgacttt gaaattccaa cttctaatat tagaaggata      480

atttgctcaa cctctggagg ttttccagag cctcacctct cctggttgga aaatggagaa      540

gaattaaatg ccatcaacac aacagtttcc caagatcctg aaactgagct ctatgctgtt      600

agcagcaaac tggatttcaa tatgacaacc aaccacagct tcatgtgtct catcaagtat      660

ggacatttaa gagtgaatca gaccttcaac tggaatacaa ccggcggtgg cggcggaggc      720

ggtggcggtt ccggagctga ggtcccggtg gtgtgggccc aggagggggc tcctgcccag      780

ctcccctgca gccccacaat ccccctccag gatctcagcc ttctgcgaag agcaggggtc      840

acttggcagc atcagccaga cagtggcccg cccgctgccg cccccggcca tcccctggcc      900

cccggccctc acccggcggc gccctcctcc tgggggccca ggccccgccg ctacacggtg      960

ctgagcgtgg gtcccggagg cctgcgcagc gggaggctgc ccctgcagcc ccgcgtccag     1020

ctggatgagc gcggccggca gcgcggggac ttctcgctat ggctgcgccc agcccggcgc     1080

gcggacgccg gcgagtaccg cgccgcggtg cacctcaggg accgcgccct ctcctgccgc     1140

ctccgtctgc gcctgggcca ggcctcgatg actgccagcc ccccaggatc tctcagagcc     1200

tccgactggg tcattttgaa ctgctccttc agccgccctg accgcccagc ctctgtgcat     1260

tggtttcgga accggggcca gggccgagtc cctgtccggg agtcccccca tcaccactta     1320

gcggaaagct tcctcttcct gccccaagtc agccccatgg actctgggcc ctggggctgc     1380

atcctcacct acagagatgg cttcaacgtc tccatcatgt ataacctcac tgttctgggt     1440

ctgctggtgc cccggggctc cgagcccaaa tcttgtgaca aaactcacac atgcccaccg     1500

tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc aaaacccaag     1560

gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac     1620

gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag     1680

acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc     1740

ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc     1800

ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtg     1860

tacaccctgc ccccatctcg ggatgagctg accaagaacc aggtcagcct gacctgcctg     1920

gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag     1980

aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctatacagc     2040

aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg     2100

catgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc gggtaaatga     2160


<210>  6
<211>  719
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTLA-4 BsB human construct wildtype translated protein sequence

<400>  6

Met Gly His Thr Arg Arg Gln Gly Thr Ser Pro Ser Lys Cys Pro Tyr 
1               5                   10                  15      


Leu Asn Phe Phe Gln Leu Leu Val Leu Ala Gly Leu Ser His Phe Cys 
            20                  25                  30          


Ser Gly Val Ile His Val Thr Lys Glu Val Lys Glu Val Ala Thr Leu 
        35                  40                  45              


Ser Cys Gly His Asn Val Ser Val Glu Glu Leu Ala Gln Thr Arg Ile 
    50                  55                  60                  


Tyr Trp Gln Lys Glu Lys Lys Met Val Leu Thr Met Met Ser Gly Asp 
65                  70                  75                  80  


Met Asn Ile Trp Pro Glu Tyr Lys Asn Arg Thr Ile Phe Asp Ile Thr 
                85                  90                  95      


Asn Asn Leu Ser Ile Val Ile Leu Ala Leu Arg Pro Ser Asp Glu Gly 
            100                 105                 110         


Thr Tyr Glu Cys Val Val Leu Lys Tyr Glu Lys Asp Ala Phe Lys Arg 
        115                 120                 125             


Glu His Leu Ala Glu Val Thr Leu Ser Val Lys Ala Asp Phe Pro Thr 
    130                 135                 140                 


Pro Ser Ile Ser Asp Phe Glu Ile Pro Thr Ser Asn Ile Arg Arg Ile 
145                 150                 155                 160 


Ile Cys Ser Thr Ser Gly Gly Phe Pro Glu Pro His Leu Ser Trp Leu 
                165                 170                 175     


Glu Asn Gly Glu Glu Leu Asn Ala Ile Asn Thr Thr Val Ser Gln Asp 
            180                 185                 190         


Pro Glu Thr Glu Leu Tyr Ala Val Ser Ser Lys Leu Asp Phe Asn Met 
        195                 200                 205             


Thr Thr Asn His Ser Phe Met Cys Leu Ile Lys Tyr Gly His Leu Arg 
    210                 215                 220                 


Val Asn Gln Thr Phe Asn Trp Asn Thr Thr Gly Gly Gly Gly Gly Gly 
225                 230                 235                 240 


Gly Gly Gly Ser Gly Ala Glu Val Pro Trp Trp Ala Gln Glu Gly Ala 
                245                 250                 255     


Pro Ala Gln Leu Pro Cys Ser Pro Thr Ile Pro Leu Gln Asp Leu Ser 
            260                 265                 270         


Leu Leu Arg Arg Ala Gly Val Thr Trp Gln His Gln Pro Asp Ser Gly 
        275                 280                 285             


Pro Pro Ala Ala Ala Pro Gly His Pro Leu Ala Pro Gly Pro His Pro 
    290                 295                 300                 


Ala Ala Pro Ser Ser Trp Gly Pro Arg Pro Arg Arg Tyr Thr Val Leu 
305                 310                 315                 320 


Ser Val Gly Pro Gly Gly Leu Arg Ser Gly Arg Leu Pro Leu Gln Pro 
                325                 330                 335     


Arg Val Gln Leu Asp Glu Arg Gly Arg Gln Arg Gly Asp Phe Ser Leu 
            340                 345                 350         


Trp Leu Arg Pro Ala Arg Arg Ala Asp Ala Gly Glu Tyr Arg Ala Ala 
        355                 360                 365             


Val His Leu Arg Asp Arg Ala Leu Ser Cys Arg Leu Arg Leu Arg Leu 
    370                 375                 380                 


Gly Gln Ala Ser Met Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser 
385                 390                 395                 400 


Asp Val Val Val Ile Leu Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro 
                405                 410                 415     


Ala Ser Val His Trp Phe Arg Asn Arg Gly Gln Gly Arg Val Pro Val 
            420                 425                 430         


Arg Glu Ser Pro His His His Leu Ala Glu Ser Phe Leu Phe Leu Pro 
        435                 440                 445             


Gln Val Ser Pro Met Asp Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr 
    450                 455                 460                 


Arg Asp Gly Phe Asn Val Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
465                 470                 475                 480 


Leu Leu Val Pro Arg Gly Ser Glu Pro Lys Ser Cys Asp Lys Thr His 
                485                 490                 495     


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
            500                 505                 510         


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
        515                 520                 525             


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
    530                 535                 540                 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
545                 550                 555                 560 


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
                565                 570                 575     


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
            580                 585                 590         


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
        595                 600                 605             


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
    610                 615                 620                 


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
625                 630                 635                 640 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
                645                 650                 655     


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
            660                 665                 670         


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
        675                 680                 685             


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
    690                 695                 700                 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
705                 710                 715                 


<210>  7
<211>  2160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTLA-4 BsB human construct variant nucleotide sequence 1

<400>  7
atgggccaca cacggaggca gggaacatca ccatccaagt gtccatacct caatttcttt       60

cagctcttgg tgctggctgg tctttctcac ttctgttcag gtgttatcca cgtgaccaag      120

gaagtgaaag aagtggcaac gctgtcctgt ggtcacaatg tttctgttga agagctggca      180

caaactcgca tctactggca aaaggagaag aaaatggtgc tgactatgat gtctggggac      240

atgaatatag cccccgagta caagaaccgg accatctttg atatcactaa taacctctcc      300

attgtgatcc tggctctgcg cccatctgac gagggcacat acgagtgtgt tgttctgaag      360

tatgaaaaag acgctttcaa gcgggaacac ctggctgaag tgacgttatc agtcaaagct      420

gacttcccta cacctagtat atctgacttt gaaattccaa cttctaatat tagaaggata      480

atttgctcaa cctctggagg ttttccagag cctcacctct cctggttgga aaatggagaa      540

gaattaaatg ccatcaacac aacagttgcc caagatcctg aaactgagct ctatgctgtt      600

agcagcaaac tggatttcaa tatgacaacc aaccacagct tcatgtgtct catcaagtat      660

ggacatttaa gagtgaatca gaccttcaac tggaatacaa ccggcggtgg cggcggaggc      720

ggtggcggtt ccggagctga ggtcccggtg gtgtgggccc aggagggggc tcctgcccag      780

ctcccctgca gccccacaat ccccctccag gatctcagcc ttctgcgaag agcaggggtc      840

acttggcagc atcagccaga cagtggcccg cccgctgccg cccccggcca tcccctggcc      900

cccggccctc acccggcggc gccctcctcc tgggggccca ggcccgagcg ctacacggtg      960

ctgagcgtgg gtcccggagg cctgcgcagc gggaggctgc ccctgcagcc ccgcgtccag     1020

ctggatgagc gcggccggca gcgcggggac ttctcgctat ggctgcgccc agcccggcgc     1080

gcggacgccg gcgagtaccg cgccgcggtg cacctcaggg accgcgccct ctcctgccgc     1140

ctccgtctgc gcctgggcca ggcctcgatg actgccagcc ccccaggatc tctcagagcc     1200

tccgactggg tcattttgaa ctgctccttc agccgccctg accgcccagc ctctgtgcat     1260

tggtttcgga accggggcca gggccgagtc cctgtccggg agtcccccca tcaccactta     1320

gcggaaagct tcctcttcct gccccaagtc agccccatgg actctgggcc ctggggctgc     1380

atcctcacct acagagatgg cttcaacgtc tccatcatgt ataacctcac tgttctgggt     1440

ctgctggtgc cccggggctc cgagcccaaa tcttgtgaca aaactcacac aagcccaccg     1500

agcccagcac ctgaactcct ggggggatcc tcagtcttcc tcttcccccc aaaacccaag     1560

gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac     1620

gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag     1680

acaaagccgc gggaggagca gtaccagagc acgtaccgtg tggtcagcgt cctcaccgtc     1740

ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc     1800

ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtg     1860

tacaccctgc ccccatctcg ggatgagctg accaagaacc aggtcagcct gacctgcctg     1920

gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag     1980

aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctatacagc     2040

aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg     2100

catgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc gggtaaatga     2160


<210>  8
<211>  719
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTLA-4 BsB human construct variant translated protein sequence 1

<400>  8

Met Gly His Thr Arg Arg Gln Gly Thr Ser Pro Ser Lys Cys Pro Tyr 
1               5                   10                  15      


Leu Asn Phe Phe Gln Leu Leu Val Leu Ala Gly Leu Ser His Phe Cys 
            20                  25                  30          


Ser Gly Val Ile His Val Thr Lys Glu Val Lys Glu Val Ala Thr Leu 
        35                  40                  45              


Ser Cys Gly His Asn Val Ser Val Glu Glu Leu Ala Gln Thr Arg Ile 
    50                  55                  60                  


Tyr Trp Gln Lys Glu Lys Lys Met Val Leu Thr Met Met Ser Gly Asp 
65                  70                  75                  80  


Met Asn Ile Ala Pro Glu Tyr Lys Asn Arg Thr Ile Phe Asp Ile Thr 
                85                  90                  95      


Asn Asn Leu Ser Ile Val Ile Leu Ala Leu Arg Pro Ser Asp Glu Gly 
            100                 105                 110         


Thr Tyr Glu Cys Val Val Leu Lys Tyr Glu Lys Asp Ala Phe Lys Arg 
        115                 120                 125             


Glu His Leu Ala Glu Val Thr Leu Ser Val Lys Ala Asp Phe Pro Thr 
    130                 135                 140                 


Pro Ser Ile Ser Asp Phe Glu Ile Pro Thr Ser Asn Ile Arg Arg Ile 
145                 150                 155                 160 


Ile Cys Ser Thr Ser Gly Gly Phe Pro Glu Pro His Leu Ser Trp Leu 
                165                 170                 175     


Glu Asn Gly Glu Glu Leu Asn Ala Ile Asn Thr Thr Val Ala Gln Asp 
            180                 185                 190         


Pro Glu Thr Glu Leu Tyr Ala Val Ser Ser Lys Leu Asp Phe Asn Met 
        195                 200                 205             


Thr Thr Asn His Ser Phe Met Cys Leu Ile Lys Tyr Gly His Leu Arg 
    210                 215                 220                 


Val Asn Gln Thr Phe Asn Trp Asn Thr Thr Gly Gly Gly Gly Gly Gly 
225                 230                 235                 240 


Gly Gly Gly Ser Gly Ala Glu Val Pro Val Val Trp Ala Gln Glu Gly 
                245                 250                 255     


Ala Pro Ala Gln Leu Pro Cys Ser Pro Thr Ile Pro Leu Gln Asp Leu 
            260                 265                 270         


Ser Leu Leu Arg Arg Ala Gly Val Thr Trp Gln His Gln Pro Asp Ser 
        275                 280                 285             


Gly Pro Pro Ala Ala Ala Pro Gly His Pro Leu Ala Pro Gly Pro His 
    290                 295                 300                 


Pro Ala Ala Pro Ser Ser Trp Gly Pro Arg Pro Glu Arg Tyr Thr Val 
305                 310                 315                 320 


Leu Ser Val Gly Pro Gly Gly Leu Arg Ser Gly Arg Leu Pro Leu Gln 
                325                 330                 335     


Pro Arg Val Gln Leu Asp Glu Arg Gly Arg Gln Arg Gly Asp Phe Ser 
            340                 345                 350         


Leu Trp Leu Arg Pro Ala Arg Arg Ala Asp Ala Gly Glu Tyr Arg Ala 
        355                 360                 365             


Ala Val His Leu Arg Asp Arg Ala Leu Ser Cys Arg Leu Arg Leu Arg 
    370                 375                 380                 


Leu Gly Gln Ala Ser Met Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala 
385                 390                 395                 400 


Ser Asp Trp Val Ile Leu Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro 
                405                 410                 415     


Ala Ser Val His Trp Phe Arg Asn Arg Gly Gln Gly Arg Val Pro Val 
            420                 425                 430         


Arg Glu Ser Pro His His His Leu Ala Glu Ser Phe Leu Phe Leu Pro 
        435                 440                 445             


Gln Val Ser Pro Met Asp Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr 
    450                 455                 460                 


Arg Asp Gly Phe Asn Val Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
465                 470                 475                 480 


Leu Leu Val Pro Arg Gly Ser Glu Pro Lys Ser Cys Asp Lys Thr His 
                485                 490                 495     


Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Ser Ser Val 
            500                 505                 510         


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
        515                 520                 525             


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
    530                 535                 540                 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
545                 550                 555                 560 


Thr Lys Pro Arg Glu Glu Gln Tyr Gln Ser Thr Tyr Arg Val Val Ser 
                565                 570                 575     


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
            580                 585                 590         


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
        595                 600                 605             


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
    610                 615                 620                 


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
625                 630                 635                 640 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
                645                 650                 655     


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
            660                 665                 670         


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
        675                 680                 685             


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
    690                 695                 700                 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
705                 710                 715                 


<210>  9
<211>  2160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTLA-4 BsB human construct variant nucleotide sequence 2

<400>  9
atgggccaca cacggaggca gggaacatca ccatccaagt gtccatacct caatttcttt       60

cagctcttgg tgctggctgg tctttctcac ttctgttcag gtgttatcca cgtgaccaag      120

gaagtgaaag aagtggcaac gctgtcctgt ggtcacaatg tttctgttga agagctggca      180

caaactcgca tctactggca aaaggagaag aaaatggtgc tgactatgat gtctggggac      240

atgaatatag cccccgagta caagaaccgg accatctttg atatcactaa taacctctcc      300

attgtgatcc tggctctgcg cccatctgac gagggcacat acgagtgtgt tgttctgaag      360

tatgaaaaag acgctttcaa gcgggaacac ctggctgaag tgacgttatc agtcaaagct      420

gacttcccta cacctagtat atctgacttt gaaattccaa cttctaatat tagaaggata      480

atttgctcaa cctctggagg ttttccagag cctcacctct cctggttgga aaatggagaa      540

gaattaaatg ccatcaacac aacagttgcc caagatcctg aaactgagct ctatgctgtt      600

gccagcaaac tggatttcaa tatgacaacc aaccacagct tcatgtgtct catcaagtat      660

ggacatttaa gagtgaatca gaccttcaac tggaatacaa ccggcggtgg cggcggaggc      720

ggtggcggtt ccggagctga ggtcccggtg gtgtgggccc aggagggggc tcctgcccag      780

ctcccctgca gccccacaat ccccctccag gatctcagcc ttctgcgaag agcaggggtc      840

acttggcagc atcagccaga cagtggcccg cccgctgccg cccccggcca tcccctggcc      900

cccggccctc acccggcggc gccctcctcc tgggggccca ggcccgagcg ctacacggtg      960

ctgagcgtgg gtcccggagg cctgcgcagc gggaggctgc ccctgcagcc ccgcgtccag     1020

ctggatgagc gcggccggca gcgcggggac ttctcgctat ggctgcgccc agcccggcgc     1080

gcggacgccg gcgagtaccg cgccgcggtg cacctcaggg accgcgccct ctcctgccgc     1140

ctccgtctgc gcctgggcca ggcctcgatg actgccagcc ccccaggatc tctcagagcc     1200

tccgactggg tcattttgaa ctgctccttc agccgccctg accgcccagc ctctgtgcat     1260

tggtttcgga accggggcca gggccgagtc cctgtccggg agtcccccca tcaccactta     1320

gcggaaagct tcctcttcct gccccaagtc agccccatgg actctgggcc ctggggctgc     1380

atcctcacct acagagatgg cttcaacgtc tccatcatgt ataacctcac tgttctgggt     1440

ctgctggtgc cccggggctc cgagcccaaa tcttgtgaca aaactcacac aagcccaccg     1500

agcccagcac ctgaactcct ggggggatcc tcagtcttcc tcttcccccc aaaacccaag     1560

gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac     1620

gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag     1680

acaaagccgc gggaggagca gtaccagagc acgtaccgtg tggtcagcgt cctcaccgtc     1740

ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc     1800

ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtg     1860

tacaccctgc ccccatctcg ggatgagctg accaagaacc aggtcagcct gacctgcctg     1920

gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag     1980

aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctatacagc     2040

aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg     2100

catgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc gggtaaatga     2160


<210>  10
<211>  719
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTLA-4 BsB human construct variant translated protein sequence 2

<400>  10

Met Gly His Thr Arg Arg Gln Gly Thr Ser Pro Ser Lys Cys Pro Tyr 
1               5                   10                  15      


Leu Asn Phe Phe Gln Leu Leu Val Leu Ala Gly Leu Ser His Phe Cys 
            20                  25                  30          


Ser Gly Val Ile His Val Thr Lys Glu Val Lys Glu Val Ala Thr Leu 
        35                  40                  45              


Ser Cys Gly His Asn Val Ser Val Glu Glu Leu Ala Gln Thr Arg Ile 
    50                  55                  60                  


Tyr Trp Gln Lys Glu Lys Lys Met Val Leu Thr Met Met Ser Gly Asp 
65                  70                  75                  80  


Met Asn Ile Ala Pro Glu Tyr Lys Asn Arg Thr Ile Phe Asp Ile Thr 
                85                  90                  95      


Asn Asn Leu Ser Ile Val Ile Leu Ala Leu Arg Pro Ser Asp Glu Gly 
            100                 105                 110         


Thr Tyr Glu Cys Val Val Leu Lys Tyr Glu Lys Asp Ala Phe Lys Arg 
        115                 120                 125             


Glu His Leu Ala Glu Val Thr Leu Ser Val Lys Ala Asp Phe Pro Thr 
    130                 135                 140                 


Pro Ser Ile Ser Asp Phe Glu Ile Pro Thr Ser Asn Ile Arg Arg Ile 
145                 150                 155                 160 


Ile Cys Ser Thr Ser Gly Gly Phe Pro Glu Pro His Leu Ser Trp Leu 
                165                 170                 175     


Glu Asn Gly Glu Glu Leu Asn Ala Ile Asn Thr Thr Val Ala Gln Asp 
            180                 185                 190         


Pro Glu Thr Glu Leu Tyr Ala Val Ala Ser Lys Leu Asp Phe Asn Met 
        195                 200                 205             


Thr Thr Asn His Ser Phe Met Cys Leu Ile Lys Tyr Gly His Leu Arg 
    210                 215                 220                 


Val Asn Gln Thr Phe Asn Trp Asn Thr Thr Gly Gly Gly Gly Gly Gly 
225                 230                 235                 240 


Gly Gly Gly Ser Gly Ala Glu Val Pro Val Val Trp Ala Gln Glu Gly 
                245                 250                 255     


Ala Pro Ala Gln Leu Pro Cys Ser Pro Thr Ile Pro Leu Gln Asp Leu 
            260                 265                 270         


Ser Leu Leu Arg Arg Ala Gly Val Thr Trp Gln His Gln Pro Asp Ser 
        275                 280                 285             


Gly Pro Pro Ala Ala Ala Pro Gly His Pro Leu Ala Pro Gly Pro His 
    290                 295                 300                 


Pro Ala Ala Pro Ser Ser Trp Gly Pro Arg Pro Glu Arg Tyr Thr Val 
305                 310                 315                 320 


Leu Ser Val Gly Pro Gly Gly Leu Arg Ser Gly Arg Leu Pro Leu Gln 
                325                 330                 335     


Pro Arg Val Gln Leu Asp Glu Arg Gly Arg Gln Arg Gly Asp Phe Ser 
            340                 345                 350         


Leu Trp Leu Arg Pro Ala Arg Arg Ala Asp Ala Gly Glu Tyr Arg Ala 
        355                 360                 365             


Ala Val His Leu Arg Asp Arg Ala Leu Ser Cys Arg Leu Arg Leu Arg 
    370                 375                 380                 


Leu Gly Gln Ala Ser Met Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala 
385                 390                 395                 400 


Ser Asp Trp Val Ile Leu Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro 
                405                 410                 415     


Ala Ser Val His Trp Phe Arg Asn Arg Gly Gln Gly Arg Val Pro Val 
            420                 425                 430         


Arg Glu Ser Pro His His His Leu Ala Glu Ser Phe Leu Phe Leu Pro 
        435                 440                 445             


Gln Val Ser Pro Met Asp Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr 
    450                 455                 460                 


Arg Asp Gly Phe Asn Val Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
465                 470                 475                 480 


Leu Leu Val Pro Arg Gly Ser Glu Pro Lys Ser Cys Asp Lys Thr His 
                485                 490                 495     


Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Ser Ser Val 
            500                 505                 510         


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
        515                 520                 525             


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
    530                 535                 540                 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
545                 550                 555                 560 


Thr Lys Pro Arg Glu Glu Gln Tyr Gln Ser Thr Tyr Arg Val Val Ser 
                565                 570                 575     


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
            580                 585                 590         


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
        595                 600                 605             


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
    610                 615                 620                 


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
625                 630                 635                 640 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
                645                 650                 655     


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
            660                 665                 670         


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
        675                 680                 685             


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
    690                 695                 700                 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
705                 710                 715                 


<210>  11
<211>  2160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTLA-4 BsB human construct variant nucleotide sequence 3

<400>  11
atgggccaca cacggaggca gggaacatca ccatccaagt gtccatacct caatttcttt       60

cagctcttgg tgctggctgg tctttctcac ttctgttcag gtgttatcca cgtgaccaag      120

gaagtgaaag aagtggcaac gctgtcctgt ggtcacaatg tttctgttga agagctggca      180

caaactcgca tctactggca aaaggagaag aaaatggtgc tgactatgat gtctggggac      240

atgaatatat ggcccgagta caagaaccgg accatctttg atatcactaa taacctctcc      300

attgtgatcc tggctctgcg cccatctgac gagggcacat acgagtgtgt tgttctgaag      360

tatgaaaaag acgctttcaa gcgggaacac ctggctgaag tgacgttatc agtcaaagct      420

gacttcccta cacctagtat atctgacttt gaaattccaa cttctaatat tagaaggata      480

atttgctcaa cctctggagg ttttccagag cctcacctct cctggttgga aaatggagaa      540

gaattaaatg ccatcaacac aacagttgcc caagatcctg aaactgccct ctatgctgtt      600

agcagcaaac tggatttcaa tatgacaacc aaccacagct tcatgtgtct catcaagtat      660

ggacatttaa gagtgaatca gaccttcaac tggaatacaa ccggcggtgg cggcggaggc      720

ggtggcggtt ccggagctga ggtcccggtg gtgtgggccc aggagggggc tcctgcccag      780

ctcccctgca gccccacaat ccccctccag gatctcagcc ttctgcgaag agcaggggtc      840

acttggcagc atcagccaga cagtggcccg cccgctgccg cccccggcca tcccctggcc      900

cccggccctc acccggcggc gccctcctcc tgggggccca ggcccgagcg ctacacggtg      960

ctgagcgtgg gtcccggagg cctgcgcagc gggaggctgc ccctgcagcc ccgcgtccag     1020

ctggatgagc gcggccggca gcgcggggac ttctcgctat ggctgcgccc agcccggcgc     1080

gcggacgccg gcgagtaccg cgccgcggtg cacctcaggg accgcgccct ctcctgccgc     1140

ctccgtctgc gcctgggcca ggcctcgatg actgccagcc ccccaggatc tctcagagcc     1200

tccgactggg tcattttgaa ctgctccttc agccgccctg accgcccagc ctctgtgcat     1260

tggtttcgga accggggcca gggccgagtc cctgtccggg agtcccccca tcaccactta     1320

gcggaaagct tcctcttcct gccccaagtc agccccatgg actctgggcc ctggggctgc     1380

atcctcacct acagagatgg cttcaacgtc tccatcatgt ataacctcac tgttctgggt     1440

ctgctggtgc cccggggctc cgagcccaaa tcttgtgaca aaactcacac aagcccaccg     1500

agcccagcac ctgaactcct ggggggatcc tcagtcttcc tcttcccccc aaaacccaag     1560

gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac     1620

gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag     1680

acaaagccgc gggaggagca gtaccagagc acgtaccgtg tggtcagcgt cctcaccgtc     1740

ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc     1800

ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtg     1860

tacaccctgc ccccatctcg ggatgagctg accaagaacc aggtcagcct gacctgcctg     1920

gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag     1980

aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctatacagc     2040

aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg     2100

catgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc gggtaaatga     2160


<210>  12
<211>  720
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTLA-4 BsB human construct variant translated protein sequence 3

<400>  12

Met Gly His Thr Arg Arg Gln Gly Thr Ser Pro Ser Lys Cys Pro Tyr 
1               5                   10                  15      


Leu Asn Phe Phe Gln Leu Leu Val Leu Ala Gly Leu Ser His Phe Cys 
            20                  25                  30          


Ser Gly Val Ile His Val Thr Lys Glu Val Lys Glu Val Ala Thr Leu 
        35                  40                  45              


Ser Cys Gly His Asn Val Ser Val Glu Glu Leu Ala Gln Thr Arg Ile 
    50                  55                  60                  


Tyr Trp Gln Lys Glu Lys Lys Met Val Leu Thr Met Met Ser Gly Asp 
65                  70                  75                  80  


Met Asn Ile Trp Pro Glu Tyr Lys Asn Arg Thr Ile Phe Asp Ile Thr 
                85                  90                  95      


Asn Asn Leu Ser Ile Val Ile Leu Ala Leu Arg Pro Ser Asp Glu Gly 
            100                 105                 110         


Thr Tyr Glu Cys Val Val Leu Lys Tyr Glu Lys Asp Ala Phe Lys Arg 
        115                 120                 125             


Glu His Leu Ala Glu Val Thr Leu Ser Val Lys Ala Asp Phe Pro Thr 
    130                 135                 140                 


Pro Ser Ile Ser Asp Phe Glu Ile Pro Thr Ser Asn Ile Arg Arg Ile 
145                 150                 155                 160 


Ile Cys Ser Thr Ser Gly Gly Phe Pro Glu Pro His Leu Ser Trp Leu 
                165                 170                 175     


Glu Asn Gly Glu Glu Leu Asn Ala Ile Asn Thr Thr Val Ala Gln Asp 
            180                 185                 190         


Pro Glu Thr Ala Leu Tyr Ala Val Ser Ser Lys Leu Asp Phe Asn Met 
        195                 200                 205             


Thr Thr Asn His Ser Phe Met Cys Leu Ile Lys Tyr Gly His Leu Arg 
    210                 215                 220                 


Val Asn Gln Thr Phe Asn Trp Asn Thr Thr Gly Gly Gly Gly Gly Gly 
225                 230                 235                 240 


Gly Gly Gly Ser Gly Ala Glu Val Pro Val Val Trp Ala Gln Glu Gly 
                245                 250                 255     


Ala Pro Ala Gln Leu Pro Cys Ser Pro Thr Ile Pro Leu Gln Asp Leu 
            260                 265                 270         


Ser Leu Leu Arg Arg Ala Gly Val Thr Trp Gln His Gln Pro Asp Ser 
        275                 280                 285             


Gly Pro Pro Ala Ala Ala Pro Gly His Pro Leu Ala Pro Gly Pro His 
    290                 295                 300                 


Pro Ala Ala Pro Ser Ser Trp Gly Pro Arg Pro Glu Arg Tyr Thr Val 
305                 310                 315                 320 


Leu Ser Val Gly Pro Gly Gly Leu Arg Ser Gly Arg Leu Pro Leu Gln 
                325                 330                 335     


Pro Arg Val Gln Leu Asp Glu Arg Gly Arg Gln Arg Gly Asp Phe Ser 
            340                 345                 350         


Leu Trp Leu Arg Pro Ala Arg Arg Ala Asp Ala Gly Glu Tyr Arg Ala 
        355                 360                 365             


Ala Val His Leu Arg Asp Arg Ala Leu Ser Cys Arg Leu Arg Leu Arg 
    370                 375                 380                 


Leu Gly Gln Ala Ser Met Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala 
385                 390                 395                 400 


Ser Asp Val Val Val Ile Leu Asn Cys Ser Phe Ser Arg Pro Asp Arg 
                405                 410                 415     


Pro Ala Ser Val His Trp Phe Arg Asn Arg Gly Gln Gly Arg Val Pro 
            420                 425                 430         


Val Arg Glu Ser Pro His His His Leu Ala Glu Ser Phe Leu Phe Leu 
        435                 440                 445             


Pro Gln Val Ser Pro Met Asp Ser Gly Pro Trp Gly Cys Ile Leu Thr 
    450                 455                 460                 


Tyr Arg Asp Gly Phe Asn Val Ser Ile Met Tyr Asn Leu Thr Val Leu 
465                 470                 475                 480 


Gly Leu Leu Val Pro Arg Gly Ser Glu Pro Lys Ser Cys Asp Lys Thr 
                485                 490                 495     


His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Ser Ser 
            500                 505                 510         


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
        515                 520                 525             


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
    530                 535                 540                 


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
545                 550                 555                 560 


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Gln Ser Thr Tyr Arg Val Val 
                565                 570                 575     


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
            580                 585                 590         


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
        595                 600                 605             


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
    610                 615                 620                 


Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 
625                 630                 635                 640 


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
                645                 650                 655     


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
            660                 665                 670         


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
        675                 680                 685             


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
    690                 695                 700                 


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
705                 710                 715                 720 


<210>  13
<211>  2160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTLA-4 BsB human construct variant nucleotide sequence 4

<400>  13
atgggccaca cacggaggca gggaacatca ccatccaagt gtccatacct caatttcttt       60

cagctcttgg tgctggctgg tctttctcac ttctgttcag gtgttatcca cgtgaccaag      120

gaagtgaaag aagtggcaac gctgtcctgt ggtcacaatg tttctgttga agagctggca      180

caaactcgca tctactggca aaaggagaag aaaatggtgc tgactatgat gtctggggac      240

atgaatatat ggcccgagta caagaaccgg accatctttg atatcactaa taacctctcc      300

attgtgatcc tggctctgcg cccatctgac gagggcacat acgagtgtgt tgttctgaag      360

tatgaaaaag acgctttcaa gcgggaacac ctggctgaag tgacgttatc agtcaaagct      420

gacttcccta cacctagtat atctgacttt gaaattccaa cttctaatat tagaaggata      480

atttgctcaa cctctggagg ttttccagag cctcacctct cctggttgga aaatggagaa      540

gaattaaatg ccatcaacac aacagttgcc caagatcctg aaactgccct ctatgctgtt      600

gccagcaaac tggatttcaa tatgacaacc aaccacagct tcatgtgtct catcaagtat      660

ggacatttaa gagtgaatca gaccttcaac tggaatacaa ccggcggtgg cggcggaggc      720

ggtggcggtt ccggagctga ggtcccggtg gtgtgggccc aggagggggc tcctgcccag      780

ctcccctgca gccccacaat ccccctccag gatctcagcc ttctgcgaag agcaggggtc      840

acttggcagc atcagccaga cagtggcccg cccgctgccg cccccggcca tcccctggcc      900

cccggccctc acccggcggc gccctcctcc tgggggccca ggcccgagcg ctacacggtg      960

ctgagcgtgg gtcccggagg cctgcgcagc gggaggctgc ccctgcagcc ccgcgtccag     1020

ctggatgagc gcggccggca gcgcggggac ttctcgctat ggctgcgccc agcccggcgc     1080

gcggacgccg gcgagtaccg cgccgcggtg cacctcaggg accgcgccct ctcctgccgc     1140

ctccgtctgc gcctgggcca ggcctcgatg actgccagcc ccccaggatc tctcagagcc     1200

tccgactggg tcattttgaa ctgctccttc agccgccctg accgcccagc ctctgtgcat     1260

tggtttcgga accggggcca gggccgagtc cctgtccggg agtcccccca tcaccactta     1320

gcggaaagct tcctcttcct gccccaagtc agccccatgg actctgggcc ctggggctgc     1380

atcctcacct acagagatgg cttcaacgtc tccatcatgt ataacctcac tgttctgggt     1440

ctgctggtgc cccggggctc cgagcccaaa tcttgtgaca aaactcacac aagcccaccg     1500

agcccagcac ctgaactcct ggggggatcc tcagtcttcc tcttcccccc aaaacccaag     1560

gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac     1620

gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag     1680

acaaagccgc gggaggagca gtaccagagc acgtaccgtg tggtcagcgt cctcaccgtc     1740

ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc     1800

ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtg     1860

tacaccctgc ccccatctcg ggatgagctg accaagaacc aggtcagcct gacctgcctg     1920

gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag     1980

aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctatacagc     2040

aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg     2100

catgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc gggtaaatga     2160


<210>  14
<211>  719
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTLA-4 BsB human construct variant translated protein sequence 4

<400>  14

Met Gly His Thr Arg Arg Gln Gly Thr Ser Pro Ser Lys Cys Pro Tyr 
1               5                   10                  15      


Leu Asn Phe Phe Gln Leu Leu Val Leu Ala Gly Leu Ser His Phe Cys 
            20                  25                  30          


Ser Gly Val Ile His Val Thr Lys Glu Val Lys Glu Val Ala Thr Leu 
        35                  40                  45              


Ser Cys Gly His Asn Val Ser Val Glu Glu Leu Ala Gln Thr Arg Ile 
    50                  55                  60                  


Tyr Trp Gln Lys Glu Lys Lys Met Val Leu Thr Met Met Ser Gly Asp 
65                  70                  75                  80  


Met Asn Ile Trp Pro Glu Tyr Lys Asn Arg Thr Ile Phe Asp Ile Thr 
                85                  90                  95      


Asn Asn Leu Ser Ile Val Ile Leu Ala Leu Arg Pro Ser Asp Glu Gly 
            100                 105                 110         


Thr Tyr Glu Cys Val Val Leu Lys Tyr Glu Lys Asp Ala Phe Lys Arg 
        115                 120                 125             


Glu His Leu Ala Glu Val Thr Leu Ser Val Lys Ala Asp Phe Pro Thr 
    130                 135                 140                 


Pro Ser Ile Ser Asp Phe Glu Ile Pro Thr Ser Asn Ile Arg Arg Ile 
145                 150                 155                 160 


Ile Cys Ser Thr Ser Gly Gly Phe Pro Glu Pro His Leu Ser Trp Leu 
                165                 170                 175     


Glu Asn Gly Glu Glu Leu Asn Ala Ile Asn Thr Thr Val Ala Gln Asp 
            180                 185                 190         


Pro Glu Thr Ala Leu Tyr Ala Val Ala Ser Lys Leu Asp Phe Asn Met 
        195                 200                 205             


Thr Thr Asn His Ser Phe Met Cys Leu Ile Lys Tyr Gly His Leu Arg 
    210                 215                 220                 


Val Asn Gln Thr Phe Asn Trp Asn Thr Thr Gly Gly Gly Gly Gly Gly 
225                 230                 235                 240 


Gly Gly Gly Ser Gly Ala Glu Val Pro Val Val Trp Ala Gln Glu Gly 
                245                 250                 255     


Ala Pro Ala Gln Leu Pro Cys Ser Pro Thr Ile Pro Leu Gln Asp Leu 
            260                 265                 270         


Ser Leu Leu Arg Arg Ala Gly Val Thr Trp Gln His Gln Pro Asp Ser 
        275                 280                 285             


Gly Pro Pro Ala Ala Ala Pro Gly His Pro Leu Ala Pro Gly Pro His 
    290                 295                 300                 


Pro Ala Ala Pro Ser Ser Trp Gly Pro Arg Pro Glu Arg Tyr Thr Val 
305                 310                 315                 320 


Leu Ser Val Gly Pro Gly Gly Leu Arg Ser Gly Arg Leu Pro Leu Gln 
                325                 330                 335     


Pro Arg Val Gln Leu Asp Glu Arg Gly Arg Gln Arg Gly Asp Phe Ser 
            340                 345                 350         


Leu Trp Leu Arg Pro Ala Arg Arg Ala Asp Ala Gly Glu Tyr Arg Ala 
        355                 360                 365             


Ala Val His Leu Arg Asp Arg Ala Leu Ser Cys Arg Leu Arg Leu Arg 
    370                 375                 380                 


Leu Gly Gln Ala Ser Met Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala 
385                 390                 395                 400 


Ser Asp Trp Val Ile Leu Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro 
                405                 410                 415     


Ala Ser Val His Trp Phe Arg Asn Arg Gly Gln Gly Arg Val Pro Val 
            420                 425                 430         


Arg Glu Ser Pro His His His Leu Ala Glu Ser Phe Leu Phe Leu Pro 
        435                 440                 445             


Gln Val Ser Pro Met Asp Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr 
    450                 455                 460                 


Arg Asp Gly Phe Asn Val Ser Ile Met Tyr Asn Leu Thr Val Leu Gly 
465                 470                 475                 480 


Leu Leu Val Pro Arg Gly Ser Glu Pro Lys Ser Cys Asp Lys Thr His 
                485                 490                 495     


Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Ser Ser Val 
            500                 505                 510         


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
        515                 520                 525             


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
    530                 535                 540                 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
545                 550                 555                 560 


Thr Lys Pro Arg Glu Glu Gln Tyr Gln Ser Thr Tyr Arg Val Val Ser 
                565                 570                 575     


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
            580                 585                 590         


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
        595                 600                 605             


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
    610                 615                 620                 


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
625                 630                 635                 640 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
                645                 650                 655     


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
            660                 665                 670         


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
        675                 680                 685             


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
    690                 695                 700                 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
705                 710                 715                 


<210>  15
<211>  288
<212>  PRT
<213>  Homo sapiens

<400>  15

Met Gly His Thr Arg Arg Gln Gly Thr Ser Pro Ser Lys Cys Pro Tyr 
1               5                   10                  15      


Leu Asn Phe Phe Gln Leu Leu Val Leu Ala Gly Leu Ser His Phe Cys 
            20                  25                  30          


Ser Gly Val Ile His Val Thr Lys Glu Val Lys Glu Val Ala Thr Leu 
        35                  40                  45              


Ser Cys Gly His Asn Val Ser Val Glu Glu Leu Ala Gln Thr Arg Ile 
    50                  55                  60                  


Tyr Trp Gln Lys Glu Lys Lys Met Val Leu Thr Met Met Ser Gly Asp 
65                  70                  75                  80  


Met Asn Ile Trp Pro Glu Tyr Lys Asn Arg Thr Ile Phe Asp Ile Thr 
                85                  90                  95      


Asn Asn Leu Ser Ile Val Ile Leu Ala Leu Arg Pro Ser Asp Glu Gly 
            100                 105                 110         


Thr Tyr Glu Cys Val Val Leu Lys Tyr Glu Lys Asp Ala Phe Lys Arg 
        115                 120                 125             


Glu His Leu Ala Glu Val Thr Leu Ser Val Lys Ala Asp Phe Pro Thr 
    130                 135                 140                 


Pro Ser Ile Ser Asp Phe Glu Ile Pro Thr Ser Asn Ile Arg Arg Ile 
145                 150                 155                 160 


Ile Cys Ser Thr Ser Gly Gly Phe Pro Glu Pro His Leu Ser Trp Leu 
                165                 170                 175     


Glu Asn Gly Glu Glu Leu Asn Ala Ile Asn Thr Thr Val Ser Gln Asp 
            180                 185                 190         


Pro Glu Thr Glu Leu Tyr Ala Val Ser Ser Lys Leu Asp Phe Asn Met 
        195                 200                 205             


Thr Thr Asn His Ser Phe Met Cys Leu Ile Lys Tyr Gly His Leu Arg 
    210                 215                 220                 


Val Asn Gln Thr Phe Asn Trp Asn Thr Thr Lys Gln Glu His Phe Pro 
225                 230                 235                 240 


Asp Asn Leu Leu Pro Ser Trp Ala Ile Thr Leu Ile Ser Val Asn Gly 
                245                 250                 255     


Ile Phe Val Ile Cys Cys Leu Thr Tyr Cys Phe Ala Pro Arg Cys Arg 
            260                 265                 270         


Glu Arg Arg Arg Asn Glu Arg Leu Arg Arg Glu Ser Val Arg Pro Val 
        275                 280                 285             


<210>  16
<211>  1485
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BsBdelta (CD80wa-Fc) DNA

<400>  16
atggcttgca attgtcagtt gatgcaggat acaccactcc tcaagtttcc atgtccaagg       60

ctcattcttc tctttgtgct gctgattcgt ctttcacaag tgtcttcaga tgttgatgaa      120

caactgtcca agtcagtgaa agataaggta ttgctgcctt gccgttacaa ctctcctcat      180

gaagatgagt ctgaagaccg aatctactgg caaaaacatg acaaagtggt gctgtctgtc      240

attgctggga aactaaaagt ggcgcccgag tataagaacc ggactttata tgacaacact      300

acctactctc ttatcatcct gggcctggtc ctttcagacc ggggcacata cagctgtgtc      360

gttcaaaaga aggaaagagg aacgtatgaa gttaaacact tggctttagt aaagttgtcc      420

atcaaagctg acttctctac ccccaacata actgagtctg gaaacccatc tgcagacact      480

aaaaggatta cctgctttgc ttccgggggt ttcccaaagc ctcgcttctc ttggttggaa      540

aatggaagag aattacctgg catcaatacg acaatttccc aggatcctga atctgaattg      600

tacaccatta gtagccaact agatttcaat acgactcgca accacaccat taagtgtctc      660

attaaatatg gagatgctca cgtgtcagag gacttcacct gggagcccag agggcccaca      720

atcaagccct gtcctccatg caaatgccca gcacctaacc tcttgggtgg accatccgtc      780

ttcatcttcc ctccaaagat caaggatgta ctcatgatct ccctgagccc catggtcaca      840

tgtgtggtgg tggatgtgag cgaggatgac ccagatgtcc agatcagctg gttcgtgaac      900

aacgtggaag tactcacagc tcagacacaa acccatagag aggattacaa cagtactctc      960

cgggtggtca gtgccctccc catccagcac caggactgga tgagtggcaa ggagttcaaa     1020

tgcaaggtca acaacaaagc cctcccagcg cccatcgaga gaaccatctc aaaacccaaa     1080

gggtcagtaa gagctccaca ggtatatgtc ttgcctccac cagaagaaga gatgactaag     1140

aaacaggtca ctctgacctg catggtcaca gacttcatgc ctgaagacat ttacgtggag     1200

tggaccaaca acgggaaaac agagctaaac tacaagaaca ctgaaccagt cctggactct     1260

gatggttctt acttcatgta cagcaagctg agagtggaaa agaagaactg ggtggaaaga     1320

aatagctact cctgctcagt ggtccacgag ggtctgcaca atcaccacac gactaagagc     1380

ttctcccgga ctccgggtaa aggcggtggc ggcggaggcg gtggcggtgg gcctgggaaa     1440

gagctgggtc tggagcccgt agcccaccat caccatcatc actga                     1485


<210>  17
<211>  494
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BsBdelta (CD80wa-Fc) Protein

<400>  17

Met Ala Cys Asn Cys Gln Leu Met Gln Asp Thr Pro Leu Leu Lys Phe 
1               5                   10                  15      


Pro Cys Pro Arg Leu Ile Leu Leu Phe Val Leu Leu Ile Arg Leu Ser 
            20                  25                  30          


Gln Val Ser Ser Asp Val Asp Glu Gln Leu Ser Lys Ser Val Lys Asp 
        35                  40                  45              


Lys Val Leu Leu Pro Cys Arg Tyr Asn Ser Pro His Glu Asp Glu Ser 
    50                  55                  60                  


Glu Asp Arg Ile Tyr Trp Gln Lys His Asp Lys Val Val Leu Ser Val 
65                  70                  75                  80  


Ile Ala Gly Lys Leu Lys Val Ala Pro Glu Tyr Lys Asn Arg Thr Leu 
                85                  90                  95      


Tyr Asp Asn Thr Thr Tyr Ser Leu Ile Ile Leu Gly Leu Val Leu Ser 
            100                 105                 110         


Asp Arg Gly Thr Tyr Ser Cys Val Val Gln Lys Lys Glu Arg Gly Thr 
        115                 120                 125             


Tyr Glu Val Lys His Leu Ala Leu Val Lys Leu Ser Ile Lys Ala Asp 
    130                 135                 140                 


Phe Ser Thr Pro Asn Ile Thr Glu Ser Gly Asn Pro Ser Ala Asp Thr 
145                 150                 155                 160 


Lys Arg Ile Thr Cys Phe Ala Ser Gly Gly Phe Pro Lys Pro Arg Phe 
                165                 170                 175     


Ser Trp Leu Glu Asn Gly Arg Glu Leu Pro Gly Ile Asn Thr Thr Ile 
            180                 185                 190         


Ser Gln Asp Pro Glu Ser Glu Leu Tyr Thr Ile Ser Ser Gln Leu Asp 
        195                 200                 205             


Phe Asn Thr Thr Arg Asn His Thr Ile Lys Cys Leu Ile Lys Tyr Gly 
    210                 215                 220                 


Asp Ala His Val Ser Glu Asp Phe Thr Trp Glu Pro Arg Gly Pro Thr 
225                 230                 235                 240 


Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly 
                245                 250                 255     


Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met 
            260                 265                 270         


Ile Ser Leu Ser Pro Met Val Thr Cys Val Val Val Asp Val Ser Glu 
        275                 280                 285             


Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val 
    290                 295                 300                 


Leu Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu 
305                 310                 315                 320 


Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly 
                325                 330                 335     


Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Ala Leu Pro Ala Pro Ile 
            340                 345                 350         


Glu Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val 
        355                 360                 365             


Tyr Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr 
    370                 375                 380                 


Leu Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu 
385                 390                 395                 400 


Trp Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro 
                405                 410                 415     


Val Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val 
            420                 425                 430         


Glu Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val 
        435                 440                 445             


His Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr 
    450                 455                 460                 


Pro Gly Lys Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Pro Gly Lys 
465                 470                 475                 480 


Glu Leu Gly Leu Glu Pro Val Ala His His His His His His 
                485                 490                 


