                         SEQUENCE LISTING

<110>  Nilles, Matthew
 
<120>  USE OF YSCF, TRUNCATED YSCF, AND YSCF HOMOLOGS AS ADJUVANTS

<130>  3128-11399PC

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  87
<212>  PRT
<213>  Yersinia pestis


<220>
<221>  MISC_FEATURE
<223>  wtYscF amino acid sequence

<400>  1

Met Ser Asn Phe Ser Gly Phe Thr Lys Gly Thr Asp Ile Ala Asp Leu 
1               5                   10                  15      


Asp Ala Val Ala Gln Thr Leu Lys Lys Pro Ala Asp Asp Ala Asn Lys 
            20                  25                  30          


Ala Val Asn Asp Ser Ile Ala Ala Leu Lys Asp Lys Pro Asp Asn Pro 
        35                  40                  45              


Ala Leu Leu Ala Asp Leu Gln His Ser Ile Asn Lys Trp Ser Val Ile 
    50                  55                  60                  


Tyr Asn Ile Asn Ser Thr Ile Val Arg Ser Met Lys Asp Leu Met Gln 
65                  70                  75                  80  


Gly Ile Leu Gln Lys Phe Pro 
                85          


<210>  2
<211>  65
<212>  PRT
<213>  Artificial

<220>
<223>  trYscF amino acid sequence

<400>  2

Leu Lys Lys Pro Ala Asp Asp Ala Asn Lys Ala Val Asn Asp Ser Ile 
1               5                   10                  15      


Ala Ala Leu Lys Asp Lys Pro Asp Asn Pro Ala Leu Leu Ala Asp Leu 
            20                  25                  30          


Gln His Ser Ile Asn Lys Trp Ser Val Ile Tyr Asn Ile Asn Ser Thr 
        35                  40                  45              


Ile Val Arg Ser Met Lys Asp Leu Met Gln Gly Ile Leu Gln Lys Phe 
    50                  55                  60                  


Pro 
65  


<210>  3
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  TrncYscFStartMT primer

<400>  3
caccctcaag aagccagcag acgatgcaaa caaagcgg                               38


<210>  4
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  TrnctYscFStopMT primer

<400>  4
ttatgggaac ttctgtagga tgccttgcat taa                                    33


<210>  5
<211>  5939
<212>  DNA
<213>  Artificial

<220>
<223>  pMNT67 plasmid


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  TOPO recognition site II

<220>
<221>  misc_feature
<222>  (23)..(151)
<223>  T7 transcription termination region

<220>
<221>  misc_feature
<222>  (62)..(81)
<223>  T7 reverse priming site

<220>
<221>  misc_feature
<222>  (551)..(1345)
<223>  kan

<220>
<221>  misc_feature
<222>  (1565)..(2206)
<223>  pBR322 Origin

<220>
<221>  misc_feature
<222>  (2574)..(2765)
<223>  ROP ORF on complementary strand

<220>
<221>  misc_feature
<222>  (4077)..(5168)
<223>  LacI ORF on complementary strand

<220>
<221>  misc_feature
<222>  (5565)..(5589)
<223>  Lac operator

<220>
<221>  misc_feature
<222>  (5634)..(5636)
<223>  Initiation ATG

<220>
<221>  misc_feature
<222>  (5646)..(5663)
<223>  Polyhistidine

<220>
<221>  misc_feature
<222>  (5703)..(5726)
<223>  Express epitope

<220>
<221>  misc_feature
<222>  (5712)..(5726)
<223>  EK recognition site

<220>
<221>  misc_feature
<222>  (5733)..(5737)
<223>  TOPO recognition site I

<220>
<221>  CDS
<222>  (5742)..(5939)
<223>  trYscF

<400>  5
aagggcgagc tcaacgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct       60

gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggagt      120

tttttgctga aaggaggaac tatatccgga tatcccgcaa gaggcccggc agtaccggca      180

taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat      240

tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc      300

attaaagctt atcgatgata agctgtcaaa catgagaatt aattcttgaa gacgaaaggg      360

cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc      420

aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca      480

ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa      540

aaggaagagt atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga      600

gaggctattc ggctatgact gggcacaact gacaatcggc tgctctgatg ccgccgtgtt      660

ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct      720

gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg      780

cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt      840

gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc      900

tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc      960

gaaacatcgc atcgagcggg cacgtactcg gatggaagcc ggtcttgtcg atcaggatga     1020

tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg     1080

catgcccgac ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc cgaatatcat     1140

ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg     1200

ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc     1260

tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta     1320

tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg     1380

acgcctaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt     1440

tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt     1500

aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt     1560

gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag     1620

cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca     1680

gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca     1740

agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg     1800

ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg     1860

cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct     1920

acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga     1980

gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc     2040

ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg     2100

agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg     2160

cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt     2220

tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc     2280

gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc     2340

ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca ctctcagtac     2400

aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg     2460

gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg     2520

ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg     2580

ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg     2640

tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga     2700

agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg     2760

gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa     2820

cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt     2880

tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt     2940

caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct     3000

gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac     3060

gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag     3120

cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc     3180

cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac     3240

ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg     3300

ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt     3360

cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc     3420

ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa     3480

tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg     3540

gtccaatgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat     3600

ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg     3660

aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga     3720

cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt     3780

tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa     3840

gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga     3900

gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga     3960

cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca     4020

tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac     4080

tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg     4140

cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg     4200

ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg     4260

ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat     4320

gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg     4380

gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca     4440

gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc     4500

cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag     4560

ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc     4620

tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa     4680

ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg     4740

caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca     4800

ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt     4860

tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg     4920

acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac     4980

tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc     5040

gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa     5100

acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca     5160

ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg     5220

cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa     5280

gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa     5340

ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca     5400

agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata     5460

ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag     5520

gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat     5580

aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata catatgcggg     5640

gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc     5700

gggatctgta cgacgatgac gataaggatc atcccttcac c ctc aag aag cca gca     5756
                                              Leu Lys Lys Pro Ala         
                                              1               5           

gac gat gca aac aaa gcg gtt aat gac tcg ata gca gca ttg aaa gat       5804
Asp Asp Ala Asn Lys Ala Val Asn Asp Ser Ile Ala Ala Leu Lys Asp           
                10                  15                  20                

aag cct gac aac ccg gcg cta ctt gct gac tta caa cat tca att aat       5852
Lys Pro Asp Asn Pro Ala Leu Leu Ala Asp Leu Gln His Ser Ile Asn           
            25                  30                  35                    

aaa tgg tcg gta att tac aat ata aac tca acc ata gtt cgt agc atg       5900
Lys Trp Ser Val Ile Tyr Asn Ile Asn Ser Thr Ile Val Arg Ser Met           
        40                  45                  50                        

aaa gac tta atg caa ggc atc cta cag aag ttc cca taa                   5939
Lys Asp Leu Met Gln Gly Ile Leu Gln Lys Phe Pro                           
    55                  60                  65                            


<210>  6
<211>  65
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  6

Leu Lys Lys Pro Ala Asp Asp Ala Asn Lys Ala Val Asn Asp Ser Ile 
1               5                   10                  15      


Ala Ala Leu Lys Asp Lys Pro Asp Asn Pro Ala Leu Leu Ala Asp Leu 
            20                  25                  30          


Gln His Ser Ile Asn Lys Trp Ser Val Ile Tyr Asn Ile Asn Ser Thr 
        35                  40                  45              


Ile Val Arg Ser Met Lys Asp Leu Met Gln Gly Ile Leu Gln Lys Phe 
    50                  55                  60                  


Pro 
65  


<210>  7
<211>  80
<212>  PRT
<213>  Salmonella enterica


<220>
<221>  MISC_FEATURE
<223>  wtPrgI amino acid sequence

<400>  7

Met Ala Thr Pro Trp Ser Gly Tyr Leu Asp Asp Val Ser Ala Lys Phe 
1               5                   10                  15      


Asp Thr Gly Val Asp Asn Leu Gln Thr Gln Val Thr Glu Ala Leu Asp 
            20                  25                  30          


Lys Leu Ala Ala Lys Pro Ser Asp Pro Ala Leu Leu Ala Ala Tyr Gln 
        35                  40                  45              


Ser Lys Leu Ser Glu Tyr Asn Leu Tyr Arg Asn Ala Gln Ser Asn Thr 
    50                  55                  60                  


Val Lys Val Phe Lys Asp Ile Asp Ala Ala Ile Ile Gln Asn Phe Arg 
65                  70                  75                  80  


<210>  8
<211>  63
<212>  PRT
<213>  Artificial

<220>
<223>  trPrgI amino acid sequence

<400>  8

Thr Gly Val Asp Asn Leu Gln Thr Gln Val Thr Glu Ala Leu Asp Lys 
1               5                   10                  15      


Leu Ala Ala Lys Pro Ser Asp Pro Ala Leu Leu Ala Ala Tyr Gln Ser 
            20                  25                  30          


Lys Leu Ser Glu Tyr Asn Leu Tyr Arg Asn Ala Gln Ser Asn Thr Val 
        35                  40                  45              


Lys Val Phe Lys Asp Ile Asp Ala Ala Ile Ile Gln Asn Phe Arg 
    50                  55                  60              


<210>  9
<211>  83
<212>  PRT
<213>  Shigella flexneri


<220>
<221>  MISC_FEATURE
<223>  wtMxiH amino acid sequence

<400>  9

Met Ser Val Thr Val Pro Asn Asp Asp Trp Thr Leu Ser Ser Leu Ser 
1               5                   10                  15      


Glu Thr Phe Asp Asp Gly Thr Gln Thr Leu Gln Gly Glu Leu Thr Leu 
            20                  25                  30          


Ala Leu Asp Lys Leu Ala Lys Asn Pro Ser Asn Pro Gln Leu Leu Ala 
        35                  40                  45              


Glu Tyr Gln Ser Lys Leu Ser Glu Tyr Thr Leu Tyr Arg Asn Ala Gln 
    50                  55                  60                  


Ser Asn Thr Val Lys Val Ile Lys Asp Val Asp Ala Ala Ile Ile Gln 
65                  70                  75                  80  


Asn Phe Arg 
            


<210>  10
<211>  65
<212>  PRT
<213>  Artificial

<220>
<223>  trMxiH amino acid sequence

<400>  10

Phe Asp Asp Gly Thr Gln Thr Leu Gln Gly Glu Leu Thr Leu Ala Leu 
1               5                   10                  15      


Asp Lys Leu Ala Lys Asn Pro Ser Asn Pro Gln Leu Leu Ala Glu Tyr 
            20                  25                  30          


Gln Ser Lys Leu Ser Glu Tyr Thr Leu Tyr Arg Asn Ala Gln Ser Asn 
        35                  40                  45              


Thr Val Lys Val Ile Lys Asp Val Asp Ala Ala Ile Ile Gln Asn Phe 
    50                  55                  60                  


Arg 
65  


<210>  11
<211>  71
<212>  PRT
<213>  Salmonella Spi2


<220>
<221>  MISC_FEATURE
<223>  wtSsaG amino acid sequence

<400>  11

Met Asp Ile Ala Gln Leu Val Asp Met Leu Ser His Met Ala His Gln 
1               5                   10                  15      


Ala Gly Gln Ala Ile Asn Asp Lys Met Asn Gly Asn Asp Leu Leu Asn 
            20                  25                  30          


Pro Glu Ser Met Ile Lys Ala Gln Phe Ala Leu Gln Gln Tyr Ser Thr 
        35                  40                  45              


Phe Ile Asn Tyr Glu Ser Ser Leu Ile Lys Met Ile Lys Asp Met Leu 
    50                  55                  60                  


Ser Gly Ile Ile Ala Lys Ile 
65                  70      


<210>  12
<211>  98
<212>  PRT
<213>  Artificial

<220>
<223>  His6-trSsaG amino acid sequence

<400>  12

Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 
1               5                   10                  15      


Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 
            20                  25                  30          


His Pro Phe Thr Leu Ser His Met Ala His Gln Ala Gly Gln Ala Ile 
        35                  40                  45              


Asn Asp Lys Met Asn Gly Asn Asp Leu Leu Asn Pro Glu Ser Met Ile 
    50                  55                  60                  


Lys Ala Gln Phe Ala Leu Gln Gln Tyr Ser Thr Phe Ile Asn Tyr Glu 
65                  70                  75                  80  


Ser Ser Leu Ile Lys Met Ile Lys Asp Met Leu Ser Gly Ile Ile Ala 
                85                  90                  95      


Lys Ile 
        


<210>  13
<211>  102
<212>  PRT
<213>  Artificial

<220>
<223>  His6-trYscF amino acid sequence

<400>  13

Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 
1               5                   10                  15      


Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 
            20                  25                  30          


His Pro Phe Thr Thr Leu Lys Lys Pro Ala Asp Asp Ala Asn Lys Ala 
        35                  40                  45              


Val Asn Asp Ser Ile Ala Ala Leu Lys Asp Lys Pro Asp Asn Pro Ala 
    50                  55                  60                  


Leu Leu Ala Asp Leu Gln His Ser Ile Asn Lys Trp Ser Val Ile Tyr 
65                  70                  75                  80  


Asn Ile Asn Ser Thr Ile Val Arg Ser Met Lys Asp Leu Met Gln Gly 
                85                  90                  95      


Ile Leu Gln Lys Phe Pro 
            100         


<210>  14
<211>  309
<212>  DNA
<213>  Artificial

<220>
<223>  His6-tr-yscF coding sequence (pET200)

<400>  14
atgcggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa       60

atgggtcggg atctgtacga cgatgacgat aaggatcatc ccttcaccac gctcaagaag      120

ccagcagacg atgcaaacaa agcggttaat gactcgatag cagcattgaa agataagcct      180

gacaacccgg cgctacttgc tgacttacaa cattcaatta ataaatggtc ggtaatttac      240

aatataaact caaccatagt tcgtagcatg aaagacttaa tgcaaggcat cctacagaag      300

ttcccataa                                                              309


<210>  15
<211>  243
<212>  DNA
<213>  Salmonella enterica


<220>
<221>  misc_feature
<223>  wt-prgI coding sequence

<400>  15
atggcaacac cttggtcagg ctatctggat gacgtctcag caaaatttga tacgggcgtt       60

gataatctac aaacgcaggt aacagaggcg ctggataaat tagcagcaaa accctccgat      120

ccggcgctac tggcggcgta tcagagtaag ctctcggaat ataacttgta ccgtaacgcg      180

caatcgaaca cggtaaaagt ctttaaggat attgatgctg ccattattca gaacttccgt      240

taa                                                                    243


<210>  16
<211>  247
<212>  DNA
<213>  Artificial

<220>
<223>  prgI DNA construct (pET200)

<400>  16
caccatggca acaccttggt caggctatct ggatgacgtc tcagcaaaat ttgatacggg       60

cgttgataat ctacaaacgc aggtaacaga ggcgctggat aaattagcag caaaaccctc      120

cgatccggcg ctactggcgg cgtatcagag taagctctcg gaatataact tgtaccgtaa      180

cgcgcaatcg aacacggtaa aagtctttaa ggatattgat gctgccatta ttcagaactt      240

ccgttaa                                                                247


<210>  17
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  wtPrgI forward primer

<400>  17
caccatggca acaccttggt c                                                 21


<210>  18
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  wtPrgI reverse primer

<400>  18
ttaacggaag ttctgaataa tggcag                                            26


<210>  19
<211>  116
<212>  PRT
<213>  Artificial

<220>
<223>  His6-PrgI amino acid sequence

<400>  19

Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 
1               5                   10                  15      


Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 
            20                  25                  30          


His Pro Phe Thr Met Ala Thr Pro Trp Ser Gly Tyr Leu Asp Asp Val 
        35                  40                  45              


Ser Ala Lys Phe Asp Thr Gly Val Asp Asn Leu Gln Thr Gln Val Thr 
    50                  55                  60                  


Glu Ala Leu Asp Lys Leu Ala Ala Lys Pro Ser Asp Pro Ala Leu Leu 
65                  70                  75                  80  


Ala Ala Tyr Gln Ser Lys Leu Ser Glu Tyr Asn Leu Tyr Arg Asn Ala 
                85                  90                  95      


Gln Ser Asn Thr Val Lys Val Phe Lys Asp Ile Asp Ala Ala Ile Ile 
            100                 105                 110         


Gln Asn Phe Arg 
        115     


<210>  20
<211>  252
<212>  DNA
<213>  Shigella flexneri


<220>
<221>  misc_feature
<223>  wt-mxiH coding sequence

<400>  20
atgagtgtta cagtaccgaa tgatgattgg acattgagtt cattatctga aacttttgat       60

gatggaactc aaacattaca aggtgaacta acattggcac tagataaatt agctaaaaat      120

ccttcgaatc cacagttgct ggctgaatac caaagtaaat tatctgaata tacattatat      180

aggaacgcgc aatccaatac agtgaaagtg attaaggatg ttgatgctgc aattattcaa      240

aacttcagat aa                                                          252


<210>  21
<211>  198
<212>  DNA
<213>  Artificial

<220>
<223>  tr-mxiH coding sequence (pET200)

<400>  21
tttgatgatg gaactcaaac attacaaggt gaactaacat tggcactaga taaattagct       60

aaaaatcctt cgaatccaca gttgctggct gaataccaaa gtaaattatc tgaatataca      120

ttatatagga acgcgcaatc caatacagtg aaagtgatta aggatgttga tgctgcaatt      180

attcaaaact tcagataa                                                    198


<210>  22
<211>  216
<212>  DNA
<213>  Salmonella Spi2


<220>
<221>  misc_feature
<223>  wt-ssaG coding sequence

<400>  22
atggatattg cacaattagt ggatatgctc tcccacatgg cgcaccaggc aggccaggcc       60

attaatgaca aaatgaatgg taatgatttg ctcaacccag aatcgatgat taaagcgcaa      120

tttgccttac agcagtattc tacatttatt aattacgaaa gttcactgat caaaatgatc      180

aaggatatgc ttagtggaat cattgctaaa atctga                                216


<210>  23
<211>  107
<212>  PRT
<213>  Artificial

<220>
<223>  His6-SsaG amino acid sequence

<400>  23

Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr 
1               5                   10                  15      


Gly Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp 
            20                  25                  30          


His Pro Phe Thr Met Asp Ile Ala Gln Leu Val Asp Met Leu Ser His 
        35                  40                  45              


Met Ala His Gln Ala Gly Gln Ala Ile Asn Asp Lys Met Asn Gly Asn 
    50                  55                  60                  


Asp Leu Leu Asn Pro Glu Ser Met Ile Lys Ala Gln Phe Ala Leu Gln 
65                  70                  75                  80  


Gln Tyr Ser Thr Phe Ile Asn Tyr Glu Ser Ser Leu Ile Lys Met Ile 
                85                  90                  95      


Lys Asp Met Leu Ser Gly Ile Ile Ala Lys Ile 
            100                 105         


<210>  24
<211>  324
<212>  DNA
<213>  Artificial

<220>
<223>  His6-SsaG coding sequence

<400>  24
atgcggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa       60

atgggtcggg atctgtacga cgatgacgat aaggatcatc ccttcaccat ggatattgca      120

caattagtgg atatgctctc ccacatggcg caccaggcag gccaggccat taatgacaaa      180

atgaatggta atgatttgct caacccagaa tcgatgatta aagcgcaatt tgccttacag      240

cagtattcta catttattaa ttacgaaagt tcactgatca aaatgatcaa ggatatgctt      300

agtggaatca ttgctaaaat ctga                                             324


<210>  25
<211>  297
<212>  DNA
<213>  Artificial

<220>
<223>  His6-tr-ssaG DNA construct

<400>  25
atgcggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa       60

atgggtcggg atctgtacga cgatgacgat aaggatcatc ccttcaccct ctcccacatg      120

gcgcaccagg caggccaggc cattaatgac aaaatgaatg gtaatgattt gctcaaccca      180

gaatcgatga ttaaagcgca atttgcctta cagcagtatt ctacatttat taattacgaa      240

agttcactga tcaaaatgat caaggatatg cttagtggaa tcattgctaa aatctga         297


<210>  26
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  Type III Needle Protein Minor Epitope


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Proline or Asparagine

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Aspartic acid or Serine

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Aspartic acid or Asparagine

<400>  26

Xaa Xaa Xaa Pro 
1               


