
                                SEQUENCE LISTING

<110> Mayo Foundation for Medical Education and Research

<120> INHIBITORS OF RTT109 AS ANTI-FUNGAL
  AGENTS

<130> 07039-1078WO1

<150> 61/499,940          
<151> 2011-06-22  

<160> 6

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 436
<212> PRT
<213> Saccharomyces cerevisiae

<400> 1
Met Ser Leu Asn Asp Phe Leu Ser Ser Val Leu Pro Val Ser Glu Gln
 1               5                  10                  15      
Phe Glu Tyr Leu Ser Leu Gln Ser Ile Pro Leu Glu Thr His Ala Val
            20                  25                  30          
Val Thr Pro Asn Lys Asp Asp Lys Arg Val Pro Lys Ser Thr Ile Lys
        35                  40                  45              
Thr Gln His Phe Phe Ser Leu Phe His Gln Gly Lys Val Phe Phe Ser
    50                  55                  60                  
Leu Glu Val Tyr Val Tyr Val Thr Leu Trp Asp Glu Ala Asp Ala Glu
65                  70                  75                  80  
Arg Leu Ile Phe Val Ser Lys Ala Asp Thr Asn Gly Tyr Cys Asn Thr
                85                  90                  95      
Arg Val Ser Val Arg Asp Ile Thr Lys Ile Ile Leu Glu Phe Ile Leu
            100                 105                 110         
Ser Ile Asp Pro Asn Tyr Tyr Leu Gln Lys Val Lys Pro Ala Ile Arg
        115                 120                 125             
Ser Tyr Lys Lys Ile Ser Pro Glu Leu Ile Ser Ala Ala Ser Thr Pro
    130                 135                 140                 
Ala Arg Thr Leu Arg Ile Leu Ala Arg Arg Leu Lys Gln Ser Gly Ser
145                 150                 155                 160 
Thr Val Leu Lys Glu Ile Glu Ser Pro Arg Phe Gln Gln Asp Leu Tyr
                165                 170                 175     
Leu Ser Phe Thr Cys Pro Arg Glu Ile Leu Thr Lys Ile Cys Leu Phe
            180                 185                 190         
Thr Arg Pro Ala Ser Gln Tyr Leu Phe Pro Asp Ser Ser Lys Asn Ser
        195                 200                 205             
Lys Lys His Ile Leu Asn Gly Glu Glu Leu Met Lys Trp Trp Gly Phe
    210                 215                 220                 
Ile Leu Asp Arg Leu Leu Ile Glu Cys Phe Gln Asn Asp Thr Gln Ala
225                 230                 235                 240 
Lys Leu Arg Ile Pro Gly Glu Asp Pro Ala Arg Val Arg Ser Tyr Leu
                245                 250                 255     
Arg Gly Met Lys Tyr Pro Leu Trp Gln Val Gly Asp Ile Phe Thr Ser
            260                 265                 270         
Lys Glu Asn Ser Leu Ala Val Tyr Asn Ile Pro Leu Phe Pro Asp Asp
        275                 280                 285             
Pro Lys Ala Arg Phe Ile His Gln Leu Ala Glu Glu Asp Arg Leu Leu
    290                 295                 300                 
Lys Val Ser Leu Ser Ser Phe Trp Ile Glu Leu Gln Glu Arg Gln Glu
305                 310                 315                 320 
Phe Lys Leu Ser Val Thr Ser Ser Val Met Gly Ile Ser Gly Tyr Ser
                325                 330                 335     
Leu Ala Thr Pro Ser Leu Phe Pro Ser Ser Ala Asp Val Ile Val Pro
            340                 345                 350         
Lys Ser Arg Lys Gln Phe Arg Ala Ile Lys Lys Tyr Ile Thr Gly Glu
        355                 360                 365             
Glu Tyr Asp Thr Glu Glu Gly Ala Ile Glu Ala Phe Thr Asn Ile Arg
    370                 375                 380                 
Asp Phe Leu Leu Leu Arg Met Ala Thr Asn Leu Gln Ser Leu Thr Gly
385                 390                 395                 400 
Lys Arg Glu His Arg Glu Arg Asn Gln Pro Val Pro Ala Ser Asn Ile
                405                 410                 415     
Asn Thr Leu Ala Ile Thr Met Leu Lys Pro Arg Lys Lys Ala Lys Ala
            420                 425                 430         
Leu Pro Lys Thr
        435     


<210> 2
<211> 430
<212> PRT
<213> Kluyveromyces lactis

<400> 2
Met Ser Leu Glu Cys Tyr Ile Lys Asp Ala Leu Pro Phe Gly His Glu
 1               5                  10                  15      
Phe Glu Ile Ile Asn Leu Gln Ser Ile Pro Arg Glu Thr Ser Pro Ile
            20                  25                  30          
Thr Phe Pro Lys Arg Glu Ala Glu Asp Val Asn Gly Lys Thr Val Lys
        35                  40                  45              
Thr Ile Lys Thr Gln His Phe Val Ala Leu Cys Phe Glu Glu Lys Val
    50                  55                  60                  
Val Phe Ala Leu Glu Ile Val Val Tyr Phe Ser Leu Asn Ser Ala Asn
65                  70                  75                  80  
Asn Gly Pro Val Glu Arg Leu Leu Phe Val Ser Lys Ala Asp Thr Asn
                85                  90                  95      
Gly Tyr Cys Asp Leu Arg Leu Asp Ile Gly Gln Ile Thr Lys Ser Ile
            100                 105                 110         
Ile Ala Tyr Ile Leu Ala Ile Ser Pro Gly His Tyr Leu Arg Ala Val
        115                 120                 125             
Lys Pro Leu Ile Arg Lys Ala Leu Ser Gly Ala Lys Ile Ile Lys Arg
    130                 135                 140                 
Ser Thr Ser Val Gln Arg Ala Leu Glu Ile Leu Ser Asn Arg Pro Leu
145                 150                 155                 160 
Asp Ser Lys Gly Ile Thr Ser Arg Val Lys Ile Pro Asp Gln Asp Leu
                165                 170                 175     
Tyr Val Ser Phe Pro Val Pro Thr Glu Ile Val Thr Lys Val Ser Leu
            180                 185                 190         
Phe Thr Arg Ala Glu Pro Gln Tyr Leu Phe Ser Asn Ser Ser Lys Asn
        195                 200                 205             
Pro Arg Lys His Val Leu Ser Gly Asp Lys Leu Leu Lys Trp Trp Leu
    210                 215                 220                 
Lys Ile Val Asp Gln Ile Val Val Glu Asp Phe Asp Asn Lys Thr Lys
225                 230                 235                 240 
Ala Thr Leu Gln Ile Pro Gly Glu Glu Lys Arg Val Ile Ser Asn Tyr
                245                 250                 255     
Leu Arg Asn Thr Ala Tyr Gln Asn Trp Thr Val Gly Asp Ile Phe Ser
            260                 265                 270         
Glu Ser Pro Gln Asp Ile Ala Leu Tyr Arg Ile Pro Leu Phe Pro Asp
        275                 280                 285             
Asp Pro Lys Gly Arg Phe Leu Asp His Leu Ile Ser Asp Gly Arg Ile
    290                 295                 300                 
Ser Lys Val Asn Val Leu Thr Phe Trp Thr Glu Leu Gln Ala Arg Gln
305                 310                 315                 320 
Glu Phe Arg Leu Gly Ser Thr Val Ser Val Ile Gly Val Ser Gly Lys
                325                 330                 335     
Tyr Ile Gly Arg Cys Asn Glu Pro Glu Arg Thr Asp Ile Ile Ile Pro
            340                 345                 350         
Thr Ser Lys Lys Asn Phe Lys Asn Leu Lys Asn Tyr Ile Thr Gly Glu
        355                 360                 365             
Glu Tyr Asp Thr Glu Glu Gly Ala Glu Glu Ala Tyr Met Asn Ile Arg
    370                 375                 380                 
Asp Ile Phe Asp Met His Tyr Ser Thr Lys Met Ile Lys Val Ile Gly
385                 390                 395                 400 
Asn His His Lys Ile Ala Thr Thr Lys Arg Pro Glu Ile Asn Thr Ile
                405                 410                 415     
Asn Val Ile Asn Thr Leu Ser Val His Arg Lys Pro Lys Ser
            420                 425                 430 


<210> 3
<211> 421
<212> PRT
<213> Ashbya gossypii

<400> 3
Met Gly Leu Lys Glu Leu Leu Ala Glu Ala Leu Pro Ala Gly His Asp
 1               5                  10                  15      
Phe Asp Val Leu His Leu Gln Ser Pro Ala Thr Glu Arg Pro Pro Leu
            20                  25                  30          
Thr Glu Ala Ser Gly Cys Ser Pro Thr Thr Ile Ala Ala Gln His Phe
        35                  40                  45              
Phe Thr Leu Ser Asn Asn Ser Lys Val Phe Tyr Ala Leu Gln Val Val
    50                  55                  60                  
Val Tyr Ile Cys Ile Glu Gly Thr Ser Gly Asp Arg Met Leu Phe Val
65                  70                  75                  80  
Ser Lys Ala Asp Thr Asn Gly Tyr Cys Asp Val Arg Val Asp Val Arg
                85                  90                  95      
Arg Ala Thr Arg Ala Leu Leu Gln Phe Leu Leu Ser Ile Asp Pro Ser
            100                 105                 110         
His Tyr Leu Gln Arg Val Arg Arg Met Arg Ile Asp Pro Ala Ala Cys
        115                 120                 125             
Lys Gly Leu Ile Thr Pro Ala Thr Asn Ala Val Asp Ala Leu Arg Ile
    130                 135                 140                 
Leu Ala Gln Arg Tyr Arg Thr Gly Asn Leu Asp Val Arg Ala Ala Ala
145                 150                 155                 160 
Asp Arg Ala Arg Tyr Thr Ser Phe Pro Cys Pro Pro Gln Tyr Thr Thr
                165                 170                 175     
Arg Leu Ala Leu Phe Thr Arg Ala Glu Pro His Tyr Leu Phe Ser Glu
            180                 185                 190         
Ser Ala Gln Asn Pro Ala Lys His Val Leu Pro Gly Asp Arg Leu Leu
        195                 200                 205             
Arg Trp Trp Leu Ala Ser Val Asp Asp Leu Leu Val Thr Leu Phe Asp
    210                 215                 220                 
Pro Ala Thr Asp Ala Ala Leu Leu Ile Pro Gly Glu Asp Ser Ala Val
225                 230                 235                 240 
Thr Ala Ala His Tyr Leu Arg Pro Val Arg Phe Pro Arg Trp Arg Val
                245                 250                 255     
Gly His Val Phe Gly Gly Ser Ala Glu Asp Pro Ala Leu Thr Arg Ile
            260                 265                 270         
Pro Arg Phe Pro Asp Asp Pro Lys Gly Arg Phe Leu Asp Asp Leu Ala
        275                 280                 285             
His Glu Gly Arg Asn Pro Ser Leu Arg Ala Phe Trp Thr Glu Leu Val
    290                 295                 300                 
Ala Arg Gln Glu Phe Arg Leu Gly Val Val Val Gly Val Val Gly Ala
305                 310                 315                 320 
Ala Gly Leu Tyr Thr Gly Pro Cys Ala Arg Pro Ala Pro Glu Asp Leu
                325                 330                 335     
Leu Cys Pro Ala Ser Pro Ala Asp Tyr Val His Val Lys His Tyr Val
            340                 345                 350         
Thr Ala Glu Glu Tyr Ala Thr Ala Ala Gly Ala Ser Glu Ala His Arg
        355                 360                 365             
Asn Leu Val Asp Cys Leu Leu Leu Arg Tyr Gly Val Ala Met Pro Arg
    370                 375                 380                 
Val Arg Gly Ala His Ser Thr Thr Pro Arg Pro Ala Pro Thr Gln Asp
385                 390                 395                 400 
Ala Thr Lys Arg Pro Ala Val Asn Asp Leu Thr Ala Arg Cys Val Arg
                405                 410                 415     
Lys Lys Pro Arg Cys
            420     


<210> 4
<211> 359
<212> PRT
<213> Candida albicans

<400> 4
Met Leu Pro Pro Asp Ile Leu Gln Asn Gly Glu Phe Glu Thr Ile Tyr
 1               5                  10                  15      
Phe Gln Thr Asn Pro Thr Tyr Ile Lys Ser Pro Ile His Ile Pro Lys
            20                  25                  30          
Ser Thr Ile Gly Lys Pro Asp Thr Val Lys Ile Arg His Phe Phe Ala
        35                  40                  45              
Leu Leu His Gln Asp Leu Val Val Leu Gly Leu Glu Val Phe Val Tyr
    50                  55                  60                  
Leu Gln Ile Tyr Ser Asp Phe Val Glu Lys Tyr Val Tyr Val Ser Lys
65                  70                  75                  80  
Cys Asp Thr Val Gly Leu Glu Lys Ser Thr Ile Lys Ile Gly Lys Val
                85                  90                  95      
Ile Gly Pro Val Leu Gln Tyr Ile Ile Asn Tyr Asn Gly Tyr Lys Ile
            100                 105                 110         
Lys Met Lys Asn Leu Asp Glu Lys Ser Lys Asp Leu Ser Asp Pro Ser
        115                 120                 125             
Thr Leu Val Arg Leu Gln Arg Leu Arg Asp Lys Leu Pro Asp Ile Tyr
    130                 135                 140                 
Pro Asn Leu Pro Tyr Tyr Asn Asp Ile Pro Pro Lys Glu Glu Cys Ile
145                 150                 155                 160 
Glu Tyr Arg Thr Leu Pro Lys Thr Gln Asn Leu Arg Leu Cys Val Phe
                165                 170                 175     
Thr Lys Pro Ala Lys Glu Tyr Leu Phe Pro Asn Ser Ala Lys Asn Pro
            180                 185                 190         
Tyr Lys Asn Leu Leu Asn Gly Gln Ser Leu Leu Arg Trp Trp Ile Ser
        195                 200                 205             
Ile Ile Asp Ser Ile Thr Lys Gly Trp Asn Asn His Lys Leu Met Ile
    210                 215                 220                 
Pro Gly Ala Asp Lys Tyr Ala Thr Arg Lys Phe Ile Glu Lys Tyr Ser
225                 230                 235                 240 
Asp Trp Ser Glu Gly His Ile Phe Lys Lys Asp Gly Leu Ala Val Gln
                245                 250                 255     
Ala Ile Pro Leu Phe Pro Asp Asp Pro Lys Gly Arg Phe Leu Glu Leu
            260                 265                 270         
Val Ile Val Glu Cys Arg Tyr Gly Lys Met Thr Val Ser Arg Phe Tyr
        275                 280                 285             
Gln Glu Leu Ala Tyr Arg Gln Glu Phe Leu Leu Gly Asp Cys Val Ser
    290                 295                 300                 
Leu Ile Gly Cys Cys Lys Glu Asn Leu Glu Val Thr Tyr His Asp Asp
305                 310                 315                 320 
Ser Val Ser Thr Val Thr Ile Ser Glu Tyr Lys Glu Phe Met Asn Ser
                325                 330                 335     
Leu Lys Ser Val Asp Phe Ser Asp Arg Val Glu Val Ser Asn Phe Val
            340                 345                 350         
Ser Asn Tyr Arg Lys Ser Lys
        355                 


<210> 5
<211> 369
<212> PRT
<213> Schizosaccharomyces pombe

<400> 5
Met Pro Asp Leu Trp Ser Glu Ser Ile Leu Glu Gly Arg Lys Leu Ser
 1               5                  10                  15      
Ile Tyr His Leu Lys Ser Thr Leu Glu Lys Cys Pro Phe Leu Phe Gly
            20                  25                  30          
Gln Ser Lys Lys Ser Lys Asp Phe Gln Phe Gly Ser His Leu Phe Leu
        35                  40                  45              
Val Glu Glu Gln Asn Val Phe Ile Phe Gly Met Glu Cys Ile Val Tyr
    50                  55                  60                  
Glu Lys Asn Lys Glu Phe Ile Val Phe Val Ser Lys Ala Asp Ser Thr
65                  70                  75                  80  
Gly Phe Gly Ser Lys Gly Val Ser Cys Asn Ser Leu Ala Phe Cys Cys
                85                  90                  95      
Leu Val Thr Leu Ile Asp Gly Leu Arg Lys Gln Gly Ala Glu Asn Val
            100                 105                 110         
Thr Leu Thr Leu Phe Ala Ile Ala Gln Gly Gln Tyr Leu Phe Pro Glu
        115                 120                 125             
Ser Val Asp Asn Gly Gln Lys His Val Leu Asn Asp Ser Gly Leu Leu
    130                 135                 140                 
Arg Trp Trp Val Asn Cys Leu Glu Lys Leu Arg Lys Tyr Tyr Thr Asp
145                 150                 155                 160 
Ser Glu Ala Pro Asn Asp Ser Glu Lys Gln Lys Asn Ser Thr Leu Leu
                165                 170                 175     
Pro Lys Ala Tyr Leu Phe Val Pro Gly Leu Glu Asn Ile Arg Ser Tyr
            180                 185                 190         
Leu Pro Asn Arg His Trp Ile Glu Ser Asn Ala Ile Thr Thr Gly Lys
        195                 200                 205             
Ala Val Glu Glu Leu Pro Arg Phe Pro Asp Asp Pro Lys Cys Arg Tyr
    210                 215                 220                 
Leu Cys Glu Leu Gln Asp Glu Lys Ser Asp Met Ser Val Glu Glu Phe
225                 230                 235                 240 
Trp Asp Thr Leu Thr Tyr Arg Gln Glu Cys Ser Ser Gly Lys Leu Val
                245                 250                 255     
Gly Phe Phe Thr Leu Gln Leu Gln Phe Tyr Gln Thr Arg Glu Phe Ile
            260                 265                 270         
Ala Lys Asp Asn Phe Gly Asp Ser Gly Val Met Ile Pro Ala Lys Leu
        275                 280                 285             
Tyr Arg Val Thr Tyr Asp Thr Leu Leu Lys His Pro Phe Gly Ser Leu
    290                 295                 300                 
Ser Asp Ala Gln Ser Ser Thr Glu Lys Phe Leu Ser Asn Thr Leu Ser
305                 310                 315                 320 
Ala Val Gln Asn Leu Lys Asp Phe His Tyr Lys Arg Tyr Lys Leu Asp
                325                 330                 335     
Ile Cys Gly Leu Ala Lys Arg Asp Asp Arg Lys Asn His Asn His Ser
            340                 345                 350         
Lys Pro Ala Thr Gln Ala Asn Ile Leu Gln Pro Arg Lys Lys Val Lys
        355                 360                 365             
Lys
    


<210> 6
<211> 587
<212> PRT
<213> Aspergillus oryzae

<400> 6
Met Thr Ile Ala Val Leu Glu Ser Leu Phe Ser His Glu Asn Tyr Gly
 1               5                  10                  15      
Asp Val Ala Asp Pro Met Ser Lys Val Asp Val Asp Leu Gly Asp Leu
            20                  25                  30          
Leu Ala Lys Val Leu Pro Thr Gly Val Lys Val Thr Ile Arg His Ile
        35                  40                  45              
Ser Ser Ala Pro Thr Pro Cys Thr Ala Leu Phe Thr Pro Pro Pro Gly
    50                  55                  60                  
Glu Glu Ser Glu Ser Thr Phe Cys Glu Asn His Phe Leu Thr Val Ser
65                  70                  75                  80  
Val Asn Ala Asp Glu His Asp Gly Pro Glu Ile Ile Val Phe Gly Ile
                85                  90                  95      
Glu Val Leu Val Tyr Ser Thr Ala His Leu Thr Thr Val Phe Val Ser
            100                 105                 110         
Lys Ala Asp Ser Thr Gly Phe Leu His Leu Leu Ile Lys Asn Ala Pro
        115                 120                 125             
Lys Val Ser Leu Ile Arg Arg Ile Ser Asn Gly Phe Leu Ser Phe Leu
    130                 135                 140                 
Val Gln Thr His Gln Arg Pro Gly Val Arg Leu Val Val Ser Leu Phe
145                 150                 155                 160 
Ala Arg Ala Gln Asn Gln Tyr Leu Phe Pro Gly Ser Ile Glu Asn Ser
                165                 170                 175     
Gly Lys His Val Leu Asp Asp Arg Gly Leu Ile Lys Trp Trp Cys Arg
            180                 185                 190         
Val Val Asp Pro Ile Leu Arg Glu Tyr Glu Pro Glu Ser Gly Ser His
        195                 200                 205             
Glu Lys Gly Leu Leu Asp Arg Ala Met Glu Ser Ala Lys Ser Ser Ala
    210                 215                 220                 
Thr Ala Phe Leu Ile Val Pro Gly Cys Asp Lys Phe Glu Thr Arg Gly
225                 230                 235                 240 
Phe Phe Pro Gly Thr Ala Lys Ser Asp Asp Lys Glu Arg Pro Arg Trp
                245                 250                 255     
Leu Asn Ser Tyr Pro Leu His Gln Leu Cys Asp Asn Thr Asn Ala Pro
            260                 265                 270         
Pro Arg Cys Leu Val Pro Arg Phe Pro Asp Asp Pro Lys Thr Arg Phe
        275                 280                 285             
Leu Leu Ile Asn Leu Asp Asp Glu Leu Pro Gln Lys Leu Glu Ala Ala
    290                 295                 300                 
Gly Ser Lys Glu Gly Ala Gly Gln Trp Arg Ser Val Lys Ser Leu Asp
305                 310                 315                 320 
Gln Phe Trp Glu Met Met Ser Phe Arg Gln Glu Cys Ser Ala Gly Arg
                325                 330                 335     
Leu Val Gly Phe Leu Trp Leu Val Ile Asn Pro Pro Gly Leu Val Asn
            340                 345                 350         
Ser Val Gln Met Thr Ser Ser Arg Pro Val Phe Lys Glu Lys Ala Glu
        355                 360                 365             
Gly Ser Leu Thr Thr Thr Val Pro Val Tyr Asp Lys Val Asp Ser Lys
    370                 375                 380                 
Pro Thr Gly Thr Ala Val Ser Val Ser Thr Asp Ser Gln Ser Ser Asp
385                 390                 395                 400 
Thr Ala Lys Asp Ser Thr Ala Glu Ala Thr Ser Gly Gly Pro Val Gln
                405                 410                 415     
Asp Pro Ser Thr Gln Thr Gly Ser Leu Ser Ser Glu Thr His Pro Lys
            420                 425                 430         
Val Gln Pro Asn Thr Asp Gln Asn Ala Phe Tyr Trp Pro Glu Ala Gly
        435                 440                 445             
Arg Gly His Ala Val Met Ser Glu Glu Asp Tyr Lys Thr Ala Ile Asn
    450                 455                 460                 
Phe Leu Leu Glu Gln Asp Phe Tyr Asn Glu Lys Val Ser Ile Ala Ser
465                 470                 475                 480 
Thr Lys Ala Trp Ser Glu Lys Val Ala Ser Ile Val Asp Gln Leu Trp
                485                 490                 495     
Val Gly Gln Gln Val Thr Gly Arg Asn Thr Ser Gly Glu Ser Ala Asp
            500                 505                 510         
Lys His Ala Pro Thr Thr Asn Ile Ile Asn Thr Gly Leu Val Arg Lys
        515                 520                 525             
Arg Lys Lys Asp Glu Pro Ser Gln Ala Thr Thr Ala Thr Ser Ala Gln
    530                 535                 540                 
Lys Glu Gly Cys Glu Gly Asn Gly Thr Val Ser Thr Ala Val Thr Ala
545                 550                 555                 560 
Glu Ala Ser Thr Thr Gly Thr Asn Glu Ser Pro Gly Val Asn Val Leu
                565                 570                 575     
Gln Ala Asn Leu Ile Arg Lys Lys Lys Lys Ala
            580                 585         

