                                SEQUENCE LISTING

<110> Wang, Yan
      Sullivan, John
      Bio-Rad Laboratories, Inc.

<120> Hybrid Polymerases Having the Ability to
  Produce Long Amplicons
  

<130> 94260-843074

<140> WO Not yet assigned       
<141> Not yet assigned  

<150> US 13/165,373       
<151> 2011-06-21  

<160> 17

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 2327
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic exemplary DNA polymerase

<400> 1
atgatcctgg atgctgacta catcactgaa gaaggcaaac cggttatccg tctgttcaaa 60
aaagagaacg gcaaatttaa gattgagcat gatcgcacct ttcgtccata catttacgct 120
ctgctgaaag atgattctaa gattgatgaa gttagaaaaa tcactggtga gcgccatggc 180
aagattgttc gtatcgttga tgcggaaaag gtagaaaaga aatttctggg cagaccaatc 240
gaggtgtgga aactgtatct cgaacatcca caagatgttc cggctattcg cgataaagtt 300
cgcgaacatc ctgcagttgt tgacatcttc gaatacgata ttccatttgc aaagcgttac 360
ctcatcgaca aaggcctgat accaatggag ggcgaggaag aactcaagat cctggcgttc 420
gatatagaaa ccctctatca cgaaggcgaa gagtttggta aaggcccaat tataatgatc 480
agctatgcag atgaaaacga agcaaaggtg attacttgga aaaacataga tctcccatac 540
gttgaggttg tatcttccga gcgcgagatg attaagcgct ttctcagagt tatccgcgag 600
aaggatccgg acgttatcat tacttataac ggcgactctt ttgacttccc atatctggtg 660
aaacgcgcag aaaaactcgg tattaaactg actatcggcc gtgatggttc cgagccgaag 720
atgcagcgtc tcggcgatat gaccgctgta gaaattaagg gtcgtatcca tttcgacctg 780
tatcatgtaa ttcgtcgtac tattaacctc ccgacttaca ctctcgaggc tgtatatgaa 840
gcaatttttg gtaagccgaa ggagaaggta tacgccgatg agattgcaga ggcgtgggaa 900
tccggtgagg gcctcgagcg tgttgcaaaa tactccatgg aagatgcaaa ggtgacttat 960
gaactcggca aagaattcct cccaatggaa atccagctct ctcgcctggt tggccaacca 1020
ctgtgggatg tttctcgttc ttccaccggt aacctcgtag agtggtttct cctgcgcaaa 1080
gcgtacgaac gcaacgaagt ggctccgaac aagccatctg aagaagagta tgaacgccgt 1140
ctccgcgagt cttacgctgg tggctatgtt aaagagccag aaaagggcct ctgggaaaac 1200
atcgtgtccc tcgattttcg ctctctgtat ccgtctatta tcattaccca caacgtgtct 1260
ccggatactc tcaaccgcga gggctgcaga gagtatgata ttgctccgga agtaggccac 1320
aagttctgca aggacttccc gggctttatt ccgtctctcc tgaaacatct gctcgaggaa 1380
cgccaaaaga ttaagactaa aatgaaggag tcccaggatc cgattgaaaa aaaaatgctc 1440
gactatcgcc aaagagcgat taaaatcctc gcaaactctt tttacggcta ttatggctat 1500
gcaaaagcac gctggtactg taaggagtgt gctgagtccg ttactgcttg gggtcgcaaa 1560
tacatcgagt tcgtgtggaa ggagctcgaa gaaaagtttg gctttaaagt tctctacatt 1620
gacactgatg gtctctatgc gactattccg ggtggtgagc ctgaggaaat taagaaaaag 1680
gctctagaat ttgtgaaata cattaactcg aagctcccgg gtctcctgga gctcgaatat 1740
gaaggctttt atgttcgcgg cttcttcgtt accaagaaga gatatgcggt gattgatgaa 1800
gaaggcaaag ttattactcg tggtctcgag attgtgcgcc gtgattggag cgaaattgcg 1860
aaagaaactc aagctagagt tctcgagact attctcaaac acggcaacgt tgaagaagct 1920
gtgaaaattg taaaagaagt aacccaaaag ctcgctaact atgaaattcc gccagagaag 1980
ctcgcgattt atgagcagat tactcgcccg ctgcatgagt ataaggcgat tggtccgcac 2040
gtggctgttg caaagaaact ggctgctaga ggcgtgaaaa ttaaaccggg tatggtaatt 2100
ggctacattg tactccgcgg cgatggtccg attagcaaac gtgcaattct agctgaggaa 2160
ttcgatccga gaaagcacaa gtatgacgca gaatattaca ttgagaacca ggtgctcccg 2220
gcggtactcc gtattctgga gggttttggc taccgtaagg aagacctccg ctggcaaaag 2280
actaaacagg ttggcctcac ttcttggctc aacattaaaa aatccgg               2327

<210> 2
<211> 775
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic exemplary DNA polymerase

<400> 2
Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile
 1               5                  10                  15      
Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Ile Glu His Asp Arg
            20                  25                  30          
Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Lys Ile
        35                  40                  45              
Asp Glu Val Arg Lys Ile Thr Gly Glu Arg His Gly Lys Ile Val Arg
    50                  55                  60                  
Ile Val Asp Ala Glu Lys Val Glu Lys Lys Phe Leu Gly Arg Pro Ile
65                  70                  75                  80  
Glu Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Ala Ile
                85                  90                  95      
Arg Asp Lys Val Arg Glu His Pro Ala Val Val Asp Ile Phe Glu Tyr
            100                 105                 110         
Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro
        115                 120                 125             
Met Glu Gly Glu Glu Glu Leu Lys Ile Leu Ala Phe Asp Ile Glu Thr
    130                 135                 140                 
Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile
145                 150                 155                 160 
Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val Ile Thr Trp Lys Asn Ile
                165                 170                 175     
Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys
            180                 185                 190         
Arg Phe Leu Arg Val Ile Arg Glu Lys Asp Pro Asp Val Ile Ile Thr
        195                 200                 205             
Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Val Lys Arg Ala Glu
    210                 215                 220                 
Lys Leu Gly Ile Lys Leu Thr Ile Gly Arg Asp Gly Ser Glu Pro Lys
225                 230                 235                 240 
Met Gln Arg Leu Gly Asp Met Thr Ala Val Glu Ile Lys Gly Arg Ile
                245                 250                 255     
His Phe Asp Leu Tyr His Val Ile Arg Arg Thr Ile Asn Leu Pro Thr
            260                 265                 270         
Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu
        275                 280                 285             
Lys Val Tyr Ala Asp Glu Ile Ala Glu Ala Trp Glu Ser Gly Glu Gly
    290                 295                 300                 
Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr
305                 310                 315                 320 
Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ile Gln Leu Ser Arg Leu
                325                 330                 335     
Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu
            340                 345                 350         
Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Val Ala
        355                 360                 365             
Pro Asn Lys Pro Ser Glu Glu Glu Tyr Glu Arg Arg Leu Arg Glu Ser
    370                 375                 380                 
Tyr Ala Gly Gly Tyr Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn
385                 390                 395                 400 
Ile Val Ser Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr
                405                 410                 415     
His Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Arg Glu Tyr
            420                 425                 430         
Asp Ile Ala Pro Glu Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly
        435                 440                 445             
Phe Ile Pro Ser Leu Leu Lys His Leu Leu Glu Glu Arg Gln Lys Ile
    450                 455                 460                 
Lys Thr Lys Met Lys Glu Ser Gln Asp Pro Ile Glu Lys Lys Met Leu
465                 470                 475                 480 
Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Phe Tyr Gly
                485                 490                 495     
Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu
            500                 505                 510         
Ser Val Thr Ala Trp Gly Arg Lys Tyr Ile Glu Phe Val Trp Lys Glu
        515                 520                 525             
Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly
    530                 535                 540                 
Leu Tyr Ala Thr Ile Pro Gly Gly Glu Pro Glu Glu Ile Lys Lys Lys
545                 550                 555                 560 
Ala Leu Glu Phe Val Lys Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu
                565                 570                 575     
Glu Leu Glu Tyr Glu Gly Phe Tyr Val Arg Gly Phe Phe Val Thr Lys
            580                 585                 590         
Lys Arg Tyr Ala Val Ile Asp Glu Glu Gly Lys Val Ile Thr Arg Gly
        595                 600                 605             
Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln
    610                 615                 620                 
Ala Arg Val Leu Glu Thr Ile Leu Lys His Gly Asn Val Glu Glu Ala
625                 630                 635                 640 
Val Lys Ile Val Lys Glu Val Thr Gln Lys Leu Ala Asn Tyr Glu Ile
                645                 650                 655     
Pro Pro Glu Lys Leu Ala Ile Tyr Glu Gln Ile Thr Arg Pro Leu His
            660                 665                 670         
Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Lys Leu Ala
        675                 680                 685             
Ala Arg Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val
    690                 695                 700                 
Leu Arg Gly Asp Gly Pro Ile Ser Lys Arg Ala Ile Leu Ala Glu Glu
705                 710                 715                 720 
Phe Asp Pro Arg Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn
                725                 730                 735     
Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Gly Phe Gly Tyr Arg
            740                 745                 750         
Lys Glu Asp Leu Arg Trp Gln Lys Thr Lys Gln Val Gly Leu Thr Ser
        755                 760                 765             
Trp Leu Asn Ile Lys Lys Ser
    770                 775 


<210> 3
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic flexible peptide linker

<400> 3
Gly Thr Gly Gly Gly Gly
 1               5      


<210> 4
<211> 63
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic exemplary non-specific DNA binding domain

<400> 4
Ala Thr Val Lys Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp Ile
 1               5                  10                  15      
Ser Lys Ile Lys Lys Val Trp Arg Val Gly Lys Met Ile Ser Phe Thr
            20                  25                  30          
Tyr Asp Glu Gly Gly Gly Lys Thr Gly Arg Gly Ala Val Ser Glu Lys
        35                  40                  45              
Asp Ala Pro Lys Glu Leu Leu Gln Met Leu Glu Lys Gln Lys Lys
    50                  55                  60              


<210> 5
<211> 844
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic exemplary hybrid DNA polymerase
      comprising non-specific DNA binding domain

<400> 5
Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile
 1               5                  10                  15      
Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Ile Glu His Asp Arg
            20                  25                  30          
Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Lys Ile
        35                  40                  45              
Asp Glu Val Arg Lys Ile Thr Gly Glu Arg His Gly Lys Ile Val Arg
    50                  55                  60                  
Ile Val Asp Ala Glu Lys Val Glu Lys Lys Phe Leu Gly Arg Pro Ile
65                  70                  75                  80  
Glu Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Ala Ile
                85                  90                  95      
Arg Asp Lys Val Arg Glu His Pro Ala Val Val Asp Ile Phe Glu Tyr
            100                 105                 110         
Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro
        115                 120                 125             
Met Glu Gly Glu Glu Glu Leu Lys Ile Leu Ala Phe Asp Ile Glu Thr
    130                 135                 140                 
Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile
145                 150                 155                 160 
Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val Ile Thr Trp Lys Asn Ile
                165                 170                 175     
Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys
            180                 185                 190         
Arg Phe Leu Arg Val Ile Arg Glu Lys Asp Pro Asp Val Ile Ile Thr
        195                 200                 205             
Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Val Lys Arg Ala Glu
    210                 215                 220                 
Lys Leu Gly Ile Lys Leu Thr Ile Gly Arg Asp Gly Ser Glu Pro Lys
225                 230                 235                 240 
Met Gln Arg Leu Gly Asp Met Thr Ala Val Glu Ile Lys Gly Arg Ile
                245                 250                 255     
His Phe Asp Leu Tyr His Val Ile Arg Arg Thr Ile Asn Leu Pro Thr
            260                 265                 270         
Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu
        275                 280                 285             
Lys Val Tyr Ala Asp Glu Ile Ala Glu Ala Trp Glu Ser Gly Glu Gly
    290                 295                 300                 
Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr
305                 310                 315                 320 
Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ile Gln Leu Ser Arg Leu
                325                 330                 335     
Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu
            340                 345                 350         
Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Val Ala
        355                 360                 365             
Pro Asn Lys Pro Ser Glu Glu Glu Tyr Glu Arg Arg Leu Arg Glu Ser
    370                 375                 380                 
Tyr Ala Gly Gly Tyr Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn
385                 390                 395                 400 
Ile Val Ser Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr
                405                 410                 415     
His Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Arg Glu Tyr
            420                 425                 430         
Asp Ile Ala Pro Glu Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly
        435                 440                 445             
Phe Ile Pro Ser Leu Leu Lys His Leu Leu Glu Glu Arg Gln Lys Ile
    450                 455                 460                 
Lys Thr Lys Met Lys Glu Ser Gln Asp Pro Ile Glu Lys Lys Met Leu
465                 470                 475                 480 
Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Phe Tyr Gly
                485                 490                 495     
Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu
            500                 505                 510         
Ser Val Thr Ala Trp Gly Arg Lys Tyr Ile Glu Phe Val Trp Lys Glu
        515                 520                 525             
Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly
    530                 535                 540                 
Leu Tyr Ala Thr Ile Pro Gly Gly Glu Pro Glu Glu Ile Lys Lys Lys
545                 550                 555                 560 
Ala Leu Glu Phe Val Lys Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu
                565                 570                 575     
Glu Leu Glu Tyr Glu Gly Phe Tyr Val Arg Gly Phe Phe Val Thr Lys
            580                 585                 590         
Lys Arg Tyr Ala Val Ile Asp Glu Glu Gly Lys Val Ile Thr Arg Gly
        595                 600                 605             
Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln
    610                 615                 620                 
Ala Arg Val Leu Glu Thr Ile Leu Lys His Gly Asn Val Glu Glu Ala
625                 630                 635                 640 
Val Lys Ile Val Lys Glu Val Thr Gln Lys Leu Ala Asn Tyr Glu Ile
                645                 650                 655     
Pro Pro Glu Lys Leu Ala Ile Tyr Glu Gln Ile Thr Arg Pro Leu His
            660                 665                 670         
Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Lys Leu Ala
        675                 680                 685             
Ala Arg Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val
    690                 695                 700                 
Leu Arg Gly Asp Gly Pro Ile Ser Lys Arg Ala Ile Leu Ala Glu Glu
705                 710                 715                 720 
Phe Asp Pro Arg Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn
                725                 730                 735     
Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Gly Phe Gly Tyr Arg
            740                 745                 750         
Lys Glu Asp Leu Arg Trp Gln Lys Thr Lys Gln Val Gly Leu Thr Ser
        755                 760                 765             
Trp Leu Asn Ile Lys Lys Ser Gly Thr Gly Gly Gly Gly Ala Thr Val
    770                 775                 780                 
Lys Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp Ile Ser Lys Ile
785                 790                 795                 800 
Lys Lys Val Trp Arg Val Gly Lys Met Ile Ser Phe Thr Tyr Asp Glu
                805                 810                 815     
Gly Gly Gly Lys Thr Gly Arg Gly Ala Val Ser Glu Lys Asp Ala Pro
            820                 825                 830         
Lys Glu Leu Leu Gln Met Leu Glu Lys Gln Lys Lys
        835                 840                 


<210> 6
<211> 2543
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic exemplary hybrid DNA polymerase
      comprising non-specific DNA binding domain

<220> 
<221> modified_base  
<222> (806)...(806)
<223> n = g, a, c or t

<400> 6
atgatcctgg atgctgacta catcactgaa gaaggcaaac cggttatccg tctgttcaaa 60
aaagagaacg gcaaatttaa gattgagcat gatcgcacct ttcgtccata catttacgct 120
ctgctgaaag atgattctaa gattgatgaa gttagaaaaa tcactggtga gcgccatggc 180
aagattgttc gtatcgttga tgcggaaaag gtagaaaaga aatttctggg cagaccaatc 240
gaggtgtgga aactgtatct cgaacatcca caagatgttc cggctattcg cgataaagtt 300
cgcgaacatc ctgcagttgt tgacatcttc gaatacgata ttccatttgc aaagcgttac 360
ctcatcgaca aaggcctgat accaatggag ggcgaggaag aactcaagat cctggcgttc 420
gatatagaaa ccctctatca cgaaggcgaa gagtttggta aaggcccaat tataatgatc 480
agctatgcag atgaaaacga agcaaaggtg attacttgga aaaacataga tctcccatac 540
gttgaggttg tatcttccga gcgcgagatg attaagcgct ttctcagagt tatccgcgag 600
aaggatccgg acgttatcat tacttataac ggcgactctt ttgacttccc atatctggtg 660
aaacgcgcag aaaaactcgg tattaaactg actatcggcc gtgatggttc cgagccgaag 720
atgcagcgtc tcggcgatat gaccgctgta gaaattaagg gtcgtatcca tttcgacctg 780
tatcatgtaa ttcgtcgtac tattanaacc tcccgactta cactctcgag gctgtatatg 840
aagcaatttt tggtaagccg aaggagaagg tatacgccga tgagattgca gaggcgtggg 900
aatccggtga gggcctcgag cgtgttgcaa aatactccat ggaagatgca aaggtgactt 960
atgaactcgg caaagaattc ctcccaatgg aaatccagct ctctcgcctg gttggccaac 1020
cactgtggga tgtttctcgt tcttccaccg gtaacctcgt agagtggttt ctcctgcgca 1080
aagcgtacga acgcaacgaa gtggctccga acaagccatc tgaagaagag tatgaacgcc 1140
gtctccgcga gtcttacgct ggtggctatg ttaaagagcc agaaaagggc ctctgggaaa 1200
acatcgtgtc cctcgatttt cgctctctgt atccgtctat tatcattacc cacaacgtgt 1260
ctccggatac tctcaaccgc gagggctgca gagagtatga tattgctccg gaagtaggcc 1320
acaagttctg caaggacttc ccgggcttta ttccgtctct cctgaaacat ctgctcgagg 1380
aacgccaaaa gattaagact aaaatgaagg agtcccagga tccgattgaa aaaaaaatgc 1440
tcgactatcg ccaaagagcg attaaaatcc tcgcaaactc tttttacggc tattatggct 1500
atgcaaaagc acgctggtac tgtaaggagt gtgctgagtc cgttactgct tggggtcgca 1560
aatacatcga gttcgtgtgg aaggagctcg aagaaaagtt tggctttaaa gttctctaca 1620
ttgacactga tggtctctat gcgactattc cgggtggtga gcctgaggaa attaagaaaa 1680
aggctctaga atttgtgaaa tacattaact cgaagctccc gggtctcctg gagctcgaat 1740
atgaaggctt ttatgttcgc ggcttcttcg ttaccaagaa gagatatgcg gtgattgatg 1800
aagaaggcaa agttattact cgtggtctcg agattgtgcg ccgtgattgg agcgaaattg 1860
cgaaagaaac tcaagctaga gttctcgaga ctattctcaa acacggcaac gttgaagaag 1920
ctgtgaaaat tgtaaaagaa gtaacccaaa agctcgctaa ctatgaaatt ccgccagaga 1980
agctcgcgat ttatgagcag attactcgcc cgctgcatga gtataaggcg attggtccgc 2040
acgtggctgt tgcaaagaaa ctggctgcta gaggcgtgaa aattaaaccg ggtatggtaa 2100
ttggctacat tgtactccgc ggcgatggtc cgattagcaa acgtgcaatt ctagctgagg 2160
aattcgatcc gagaaagcac aagtatgacg cagaatatta cattgagaac caggtgctcc 2220
cggcggtact ccgtattctg gagggttttg gctaccgtaa ggaagacctc cgctggcaaa 2280
agactaaaca ggttggcctc acttcttggc tcaacattaa aaaatccggt accggcggtg 2340
gcggtgcaac cgtaaagttc aagtacaaag gcgaagaaaa agaggtagac atctccaaga 2400
tcaagaaagt atggcgtgtg ggcaagatga tctccttcac ctacgacgag ggcggtggca 2460
agaccggccg tggtgcggta agcgaaaagg acgcgccgaa ggagctgctg cagatgctgg 2520
agaagcagaa aaagtgagct agc                                         2543

<210> 7
<211> 775
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic control DNA polymerase

<400> 7
Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile
 1               5                  10                  15      
Arg Leu Phe Lys Lys Glu Asn Gly Glu Phe Lys Ile Glu His Asp Arg
            20                  25                  30          
Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Lys Ile
        35                  40                  45              
Glu Glu Val Lys Lys Ile Thr Ala Glu Arg His Gly Lys Ile Val Arg
    50                  55                  60                  
Ile Val Asp Ala Glu Lys Val Glu Lys Lys Phe Leu Gly Arg Pro Ile
65                  70                  75                  80  
Thr Val Trp Arg Leu Tyr Phe Glu His Pro Gln Asp Val Pro Thr Ile
                85                  90                  95      
Arg Glu Lys Ile Arg Glu His Ser Ala Val Val Asp Ile Phe Glu Tyr
            100                 105                 110         
Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro
        115                 120                 125             
Met Glu Gly Asp Glu Glu Leu Lys Leu Leu Ala Phe Asp Ile Glu Thr
    130                 135                 140                 
Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile
145                 150                 155                 160 
Ser Tyr Ala Asp Glu Glu Glu Ala Lys Val Ile Thr Trp Lys Lys Ile
                165                 170                 175     
Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys
            180                 185                 190         
Arg Phe Leu Lys Ile Ile Arg Glu Lys Asp Pro Asp Ile Ile Ile Thr
        195                 200                 205             
Tyr Asn Gly Asp Ser Phe Asp Leu Pro Tyr Leu Ala Lys Arg Ala Glu
    210                 215                 220                 
Lys Leu Gly Ile Lys Leu Thr Ile Gly Arg Asp Gly Ser Glu Pro Lys
225                 230                 235                 240 
Met Gln Arg Ile Gly Asp Met Thr Ala Val Glu Val Lys Gly Arg Ile
                245                 250                 255     
His Phe Asp Leu Tyr His Val Ile Arg Arg Thr Ile Asn Leu Pro Thr
            260                 265                 270         
Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu
        275                 280                 285             
Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Thr Gly Glu Gly
    290                 295                 300                 
Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Ala Thr Tyr
305                 310                 315                 320 
Glu Leu Gly Lys Glu Phe Phe Pro Met Glu Ala Gln Leu Ser Arg Leu
                325                 330                 335     
Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu
            340                 345                 350         
Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala
        355                 360                 365             
Pro Asn Lys Pro Asp Glu Arg Glu Tyr Glu Arg Arg Leu Arg Glu Ser
    370                 375                 380                 
Tyr Ala Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn
385                 390                 395                 400 
Ile Val Ser Leu Asp Phe Arg Ala Leu Tyr Pro Ser Ile Ile Ile Thr
                405                 410                 415     
His Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Arg Asn Tyr
            420                 425                 430         
Asp Val Ala Pro Glu Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly
        435                 440                 445             
Phe Ile Pro Ser Leu Leu Lys Arg Leu Leu Asp Glu Arg Gln Lys Ile
    450                 455                 460                 
Lys Thr Lys Met Lys Ala Ser Gln Asp Pro Ile Glu Lys Ile Met Leu
465                 470                 475                 480 
Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly
                485                 490                 495     
Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu
            500                 505                 510         
Ser Val Thr Ala Trp Gly Arg Glu Tyr Ile Glu Phe Val Trp Lys Glu
        515                 520                 525             
Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly
    530                 535                 540                 
Leu Tyr Ala Thr Ile Pro Gly Gly Lys Ser Glu Glu Ile Lys Lys Lys
545                 550                 555                 560 
Ala Leu Glu Phe Val Asp Tyr Ile Asn Ala Lys Leu Pro Gly Leu Leu
                565                 570                 575     
Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys
            580                 585                 590         
Lys Lys Tyr Ala Leu Ile Asp Glu Glu Gly Lys Ile Ile Thr Arg Gly
        595                 600                 605             
Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln
    610                 615                 620                 
Ala Arg Val Leu Glu Ala Ile Leu Lys His Gly Asn Val Glu Glu Ala
625                 630                 635                 640 
Val Arg Ile Val Lys Glu Val Thr Gln Lys Leu Ser Lys Tyr Glu Ile
                645                 650                 655     
Pro Pro Glu Lys Leu Ala Ile Tyr Glu Gln Ile Thr Arg Pro Leu His
            660                 665                 670         
Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Arg Leu Ala
        675                 680                 685             
Ala Lys Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val
    690                 695                 700                 
Leu Arg Gly Asp Gly Pro Ile Ser Asn Arg Ala Ile Leu Ala Glu Glu
705                 710                 715                 720 
Tyr Asp Pro Arg Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn
                725                 730                 735     
Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Gly Phe Gly Tyr Arg
            740                 745                 750         
Lys Glu Asp Leu Arg Trp Gln Lys Thr Lys Gln Thr Gly Leu Thr Ser
        755                 760                 765             
Trp Leu Asn Ile Lys Lys Ser
    770                 775 


<210> 8
<211> 844
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic control hybrid DNA polymerase with Sso7d
      DNA binding domain

<400> 8
Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile
 1               5                  10                  15      
Arg Leu Phe Lys Lys Glu Asn Gly Glu Phe Lys Ile Glu His Asp Arg
            20                  25                  30          
Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Lys Ile
        35                  40                  45              
Glu Glu Val Lys Lys Ile Thr Ala Glu Arg His Gly Lys Ile Val Arg
    50                  55                  60                  
Ile Val Asp Ala Glu Lys Val Glu Lys Lys Phe Leu Gly Arg Pro Ile
65                  70                  75                  80  
Thr Val Trp Arg Leu Tyr Phe Glu His Pro Gln Asp Val Pro Thr Ile
                85                  90                  95      
Arg Glu Lys Ile Arg Glu His Ser Ala Val Val Asp Ile Phe Glu Tyr
            100                 105                 110         
Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro
        115                 120                 125             
Met Glu Gly Asp Glu Glu Leu Lys Leu Leu Ala Phe Asp Ile Glu Thr
    130                 135                 140                 
Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile
145                 150                 155                 160 
Ser Tyr Ala Asp Glu Glu Glu Ala Lys Val Ile Thr Trp Lys Lys Ile
                165                 170                 175     
Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys
            180                 185                 190         
Arg Phe Leu Lys Ile Ile Arg Glu Lys Asp Pro Asp Ile Ile Ile Thr
        195                 200                 205             
Tyr Asn Gly Asp Ser Phe Asp Leu Pro Tyr Leu Ala Lys Arg Ala Glu
    210                 215                 220                 
Lys Leu Gly Ile Lys Leu Thr Ile Gly Arg Asp Gly Ser Glu Pro Lys
225                 230                 235                 240 
Met Gln Arg Ile Gly Asp Met Thr Ala Val Glu Val Lys Gly Arg Ile
                245                 250                 255     
His Phe Asp Leu Tyr His Val Ile Arg Arg Thr Ile Asn Leu Pro Thr
            260                 265                 270         
Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu
        275                 280                 285             
Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Thr Gly Glu Gly
    290                 295                 300                 
Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Ala Thr Tyr
305                 310                 315                 320 
Glu Leu Gly Lys Glu Phe Phe Pro Met Glu Ala Gln Leu Ser Arg Leu
                325                 330                 335     
Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu
            340                 345                 350         
Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala
        355                 360                 365             
Pro Asn Lys Pro Asp Glu Arg Glu Tyr Glu Arg Arg Leu Arg Glu Ser
    370                 375                 380                 
Tyr Ala Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn
385                 390                 395                 400 
Ile Val Ser Leu Asp Phe Arg Ala Leu Tyr Pro Ser Ile Ile Ile Thr
                405                 410                 415     
His Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Arg Asn Tyr
            420                 425                 430         
Asp Val Ala Pro Glu Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly
        435                 440                 445             
Phe Ile Pro Ser Leu Leu Lys Arg Leu Leu Asp Glu Arg Gln Lys Ile
    450                 455                 460                 
Lys Thr Lys Met Lys Ala Ser Gln Asp Pro Ile Glu Lys Ile Met Leu
465                 470                 475                 480 
Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly
                485                 490                 495     
Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu
            500                 505                 510         
Ser Val Thr Ala Trp Gly Arg Glu Tyr Ile Glu Phe Val Trp Lys Glu
        515                 520                 525             
Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly
    530                 535                 540                 
Leu Tyr Ala Thr Ile Pro Gly Gly Lys Ser Glu Glu Ile Lys Lys Lys
545                 550                 555                 560 
Ala Leu Glu Phe Val Asp Tyr Ile Asn Ala Lys Leu Pro Gly Leu Leu
                565                 570                 575     
Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys
            580                 585                 590         
Lys Lys Tyr Ala Leu Ile Asp Glu Glu Gly Lys Ile Ile Thr Arg Gly
        595                 600                 605             
Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln
    610                 615                 620                 
Ala Arg Val Leu Glu Ala Ile Leu Lys His Gly Asn Val Glu Glu Ala
625                 630                 635                 640 
Val Arg Ile Val Lys Glu Val Thr Gln Lys Leu Ser Lys Tyr Glu Ile
                645                 650                 655     
Pro Pro Glu Lys Leu Ala Ile Tyr Glu Gln Ile Thr Arg Pro Leu His
            660                 665                 670         
Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Arg Leu Ala
        675                 680                 685             
Ala Lys Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val
    690                 695                 700                 
Leu Arg Gly Asp Gly Pro Ile Ser Asn Arg Ala Ile Leu Ala Glu Glu
705                 710                 715                 720 
Tyr Asp Pro Arg Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn
                725                 730                 735     
Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Gly Phe Gly Tyr Arg
            740                 745                 750         
Lys Glu Asp Leu Arg Trp Gln Lys Thr Lys Gln Thr Gly Leu Thr Ser
        755                 760                 765             
Trp Leu Asn Ile Lys Lys Ser Gly Thr Gly Gly Gly Gly Ala Thr Val
    770                 775                 780                 
Lys Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp Ile Ser Lys Ile
785                 790                 795                 800 
Lys Lys Val Trp Arg Val Gly Lys Met Ile Ser Phe Thr Tyr Asp Glu
                805                 810                 815     
Gly Gly Gly Lys Thr Gly Arg Gly Ala Val Ser Glu Lys Asp Ala Pro
            820                 825                 830         
Lys Glu Leu Leu Gln Met Leu Glu Lys Gln Lys Lys
        835                 840                 


<210> 9
<211> 25
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic conserved DNA polymerase sequence

<400> 9
Tyr Gly Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys
 1               5                  10                  15      
Ala Glu Ser Val Thr Ala Trp Gly Arg
            20                  25  


<210> 10
<211> 64
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic Ss07d/Ssh7A/SsoP2 DNA binding domain

<400> 10
Met Ala Thr Val Lys Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp
 1               5                  10                  15      
Ile Ser Lys Ile Lys Lys Val Trp Arg Val Gly Lys Met Ile Ser Phe
            20                  25                  30          
Thr Tyr Asp Glu Gly Gly Gly Lys Thr Gly Arg Gly Ala Val Ser Glu
        35                  40                  45              
Lys Asp Ala Pro Lys Glu Leu Leu Gln Met Leu Glu Lys Gln Lys Lys
    50                  55                  60                  


<210> 11
<211> 64
<212> PRT
<213> Sulfolobus shibatae

<220> 
<223> Ssh7b DNA-binding protein, Sso7-like protein

<400> 11
Met Val Thr Val Lys Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp
 1               5                  10                  15      
Thr Ser Lys Ile Lys Lys Val Trp Arg Val Gly Lys Met Ile Ser Phe
            20                  25                  30          
Thr Tyr Asp Glu Gly Gly Gly Lys Thr Gly Arg Gly Ala Val Ser Glu
        35                  40                  45              
Lys Asp Ala Pro Lys Glu Leu Leu Gln Met Leu Glu Lys Gln Lys Lys
    50                  55                  60                  


<210> 12
<211> 66
<212> PRT
<213> Sulfolobus sp.

<220> 
<223> Sac7d DNA-binding protein, Sso7-like protein

<400> 12
Met Val Lys Val Lys Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp
 1               5                  10                  15      
Thr Ser Lys Ile Lys Lys Val Trp Arg Val Gly Lys Met Val Ser Phe
            20                  25                  30          
Thr Tyr Asp Asp Asn Gly Lys Thr Gly Arg Gly Ala Val Ser Glu Lys
        35                  40                  45              
Asp Ala Pro Lys Glu Leu Leu Asp Met Leu Ala Arg Ala Glu Arg Glu
    50                  55                  60                  
Lys Lys
65      


<210> 13
<211> 65
<212> PRT
<213> Sulfolobus acidocaldarius

<220> 
<223> Sulfolobus acidocaldarius DSM 639 Sac7e
      DNA-binding protein, Sso7-like protein

<400> 13
Met Ala Lys Val Arg Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp
 1               5                  10                  15      
Thr Ser Lys Ile Lys Lys Val Trp Arg Val Gly Lys Met Val Ser Phe
            20                  25                  30          
Thr Tyr Asp Asp Asn Gly Lys Thr Gly Arg Gly Ala Val Ser Glu Lys
        35                  40                  45              
Asp Ala Pro Lys Glu Leu Met Asp Met Leu Ala Arg Ala Glu Lys Lys
    50                  55                  60                  
Lys
65  


<210> 14
<211> 64
<212> PRT
<213> Sulfolobus tokodaii

<220> 
<223> Sulfolobus tokodaii strain 7 Sto7e DNA-binding
      protein, Sso7-like protein

<400> 14
Met Val Thr Val Lys Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp
 1               5                  10                  15      
Ile Ser Lys Ile Lys Lys Val Trp Arg Val Gly Lys Met Ile Ser Phe
            20                  25                  30          
Thr Tyr Asp Asp Asn Gly Lys Thr Gly Arg Gly Ala Val Ser Glu Lys
        35                  40                  45              
Asp Ala Pro Lys Glu Leu Leu Gln Met Leu Glu Lys Ser Gly Lys Lys
    50                  55                  60                  


<210> 15
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic 6-His epitope tag

<400> 15
His His His His His His
 1               5      


<210> 16
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic anti-DYKDDDDK epitope tag

<400> 16
Asp Tyr Lys Asp Asp Asp Asp Lys
 1               5              


<210> 17
<211> 775
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic consensus DNA polymerase sequence

<220> 
<221> VARIANT        
<222> (25)...(25)
<223> Xaa = Lys or Glu

<220> 
<221> VARIANT        
<222> (49)...(49)
<223> Xaa = Asp or Glu

<220> 
<221> VARIANT        
<222> (52)...(52)
<223> Xaa = Arg or Lys

<220> 
<221> VARIANT        
<222> (56)...(56)
<223> Xaa = Gly or Ala

<220> 
<221> VARIANT        
<222> (81)...(81)
<223> Xaa = Glu or Thr

<220> 
<221> VARIANT        
<222> (84)...(84)
<223> Xaa = Lys or Arg

<220> 
<221> VARIANT        
<222> (87)...(87)
<223> Xaa = Leu or Phe

<220> 
<221> VARIANT        
<222> (95)...(95)
<223> Xaa = Ala or Thr

<220> 
<221> VARIANT        
<222> (98)...(98)
<223> Xaa = Asp or Glu

<220> 
<221> VARIANT        
<222> (100)...(100)
<223> Xaa = Val or Ile

<220> 
<221> VARIANT        
<222> (104)...(104)
<223> Xaa = Pro or Ser

<220> 
<221> VARIANT        
<222> (132)...(132)
<223> Xaa = Glu or Asp

<220> 
<221> VARIANT        
<222> (137)...(137)
<223> Xaa = Ile or Leu

<220> 
<221> VARIANT        
<222> (166)...(166)
<223> Xaa = Asn or Glu

<220> 
<221> VARIANT        
<222> (175)...(175)
<223> Xaa = Asn or Lys

<220> 
<221> VARIANT        
<222> (196)...(196)
<223> Xaa = Arg or Lys

<220> 
<221> VARIANT        
<222> (197)...(197)
<223> Xaa = Val or Ile

<220> 
<221> VARIANT        
<222> (205)...(205)
<223> Xaa = Val or Ile

<220> 
<221> VARIANT        
<222> (216)...(216)
<223> Xaa = Phe or Leu

<220> 
<221> VARIANT        
<222> (220)...(220)
<223> Xaa = Val or Ala

<220> 
<221> VARIANT        
<222> (244)...(244)
<223> Xaa = Leu or Ile

<220> 
<221> VARIANT        
<222> (252)...(252)
<223> Xaa = Ile or Val

<220> 
<221> VARIANT        
<222> (297)...(297)
<223> Xaa = Glu or Lys

<220> 
<221> VARIANT        
<222> (301)...(301)
<223> Xaa = Ser or Thr

<220> 
<221> VARIANT        
<222> (318)...(318)
<223> Xaa = Val or Ala

<220> 
<221> VARIANT        
<222> (327)...(327)
<223> Xaa = Leu or Phe

<220> 
<221> VARIANT        
<222> (331)...(331)
<223> Xaa = Ile or Ala

<220> 
<221> VARIANT        
<222> (367)...(367)
<223> Xaa = Val or Leu

<220> 
<221> VARIANT        
<222> (373)...(373)
<223> Xaa = Ser or Asp

<220> 
<221> VARIANT        
<222> (375)...(375)
<223> Xaa = Glu or Arg

<220> 
<221> VARIANT        
<222> (389)...(389)
<223> Xaa = Tyr or Phe

<220> 
<221> VARIANT        
<222> (408)...(408)
<223> Xaa = Ser or Thr

<220> 
<221> VARIANT        
<222> (431)...(431)
<223> Xaa = Glu or Asn

<220> 
<221> VARIANT        
<222> (434)...(434)
<223> Xaa = Ile or Val

<220> 
<221> VARIANT        
<222> (456)...(456)
<223> Xaa = His or Arg

<220> 
<221> VARIANT        
<222> (459)...(459)
<223> Xaa = Glu or Asp

<220> 
<221> VARIANT        
<222> (470)...(470)
<223> Xaa = Glu or Ala

<220> 
<221> VARIANT        
<222> (478)...(478)
<223> Xaa = Lys or Ile

<220> 
<221> VARIANT        
<222> (494)...(494)
<223> Xaa = Phe or Tyr

<220> 
<221> VARIANT        
<222> (520)...(520)
<223> Xaa = Lys or Glu

<220> 
<221> VARIANT        
<222> (553)...(553)
<223> Xaa = Glu or Lys

<220> 
<221> VARIANT        
<222> (554)...(554)
<223> Xaa = Pro or Ser

<220> 
<221> VARIANT        
<222> (566)...(566)
<223> Xaa = Lys or Asp

<220> 
<221> VARIANT        
<222> (570)...(570)
<223> Xaa = Ser or Ala

<220> 
<221> VARIANT        
<222> (585)...(585)
<223> Xaa = Val or Lys

<220> 
<221> VARIANT        
<222> (594)...(594)
<223> Xaa = Arg or Lys

<220> 
<221> VARIANT        
<222> (597)...(597)
<223> Xaa = Val or Leu

<220> 
<221> VARIANT        
<222> (604)...(604)
<223> Xaa = Val or Ile

<220> 
<221> VARIANT        
<222> (630)...(630)
<223> Xaa = Thr or Ala

<220> 
<221> VARIANT        
<222> (642)...(642)
<223> Xaa = Lys or Arg

<220> 
<221> VARIANT        
<222> (652)...(652)
<223> Xaa = Ala or Ser

<220> 
<221> VARIANT        
<222> (653)...(653)
<223> Xaa = Asn or Lys

<220> 
<221> VARIANT        
<222> (686)...(686)
<223> Xaa = Lys or Arg

<220> 
<221> VARIANT        
<222> (690)...(690)
<223> Xaa = Arg or Lys

<220> 
<221> VARIANT        
<222> (713)...(713)
<223> Xaa = Lys or Asn

<220> 
<221> VARIANT        
<222> (721)...(721)
<223> Xaa = Phe or Tyr

<220> 
<221> VARIANT        
<222> (764)...(764)
<223> Xaa = Val or Thr

<400> 17
Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile
 1               5                  10                  15      
Arg Leu Phe Lys Lys Glu Asn Gly Xaa Phe Lys Ile Glu His Asp Arg
            20                  25                  30          
Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Lys Ile
        35                  40                  45              
Xaa Glu Val Xaa Lys Ile Thr Xaa Glu Arg His Gly Lys Ile Val Arg
    50                  55                  60                  
Ile Val Asp Ala Glu Lys Val Glu Lys Lys Phe Leu Gly Arg Pro Ile
65                  70                  75                  80  
Xaa Val Trp Xaa Leu Tyr Xaa Glu His Pro Gln Asp Val Pro Xaa Ile
                85                  90                  95      
Arg Xaa Lys Xaa Arg Glu His Xaa Ala Val Val Asp Ile Phe Glu Tyr
            100                 105                 110         
Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro
        115                 120                 125             
Met Glu Gly Xaa Glu Glu Leu Lys Xaa Leu Ala Phe Asp Ile Glu Thr
    130                 135                 140                 
Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile
145                 150                 155                 160 
Ser Tyr Ala Asp Glu Xaa Glu Ala Lys Val Ile Thr Trp Lys Xaa Ile
                165                 170                 175     
Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys
            180                 185                 190         
Arg Phe Leu Xaa Xaa Ile Arg Glu Lys Asp Pro Asp Xaa Ile Ile Thr
        195                 200                 205             
Tyr Asn Gly Asp Ser Phe Asp Xaa Pro Tyr Leu Xaa Lys Arg Ala Glu
    210                 215                 220                 
Lys Leu Gly Ile Lys Leu Thr Ile Gly Arg Asp Gly Ser Glu Pro Lys
225                 230                 235                 240 
Met Gln Arg Xaa Gly Asp Met Thr Ala Val Glu Xaa Lys Gly Arg Ile
                245                 250                 255     
His Phe Asp Leu Tyr His Val Ile Arg Arg Thr Ile Asn Leu Pro Thr
            260                 265                 270         
Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu
        275                 280                 285             
Lys Val Tyr Ala Asp Glu Ile Ala Xaa Ala Trp Glu Xaa Gly Glu Gly
    290                 295                 300                 
Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Xaa Thr Tyr
305                 310                 315                 320 
Glu Leu Gly Lys Glu Phe Xaa Pro Met Glu Xaa Gln Leu Ser Arg Leu
                325                 330                 335     
Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu
            340                 345                 350         
Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Xaa Ala
        355                 360                 365             
Pro Asn Lys Pro Xaa Glu Xaa Glu Tyr Glu Arg Arg Leu Arg Glu Ser
    370                 375                 380                 
Tyr Ala Gly Gly Xaa Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn
385                 390                 395                 400 
Ile Val Ser Leu Asp Phe Arg Xaa Leu Tyr Pro Ser Ile Ile Ile Thr
                405                 410                 415     
His Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Arg Xaa Tyr
            420                 425                 430         
Asp Xaa Ala Pro Glu Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly
        435                 440                 445             
Phe Ile Pro Ser Leu Leu Lys Xaa Leu Leu Xaa Glu Arg Gln Lys Ile
    450                 455                 460                 
Lys Thr Lys Met Lys Xaa Ser Gln Asp Pro Ile Glu Lys Xaa Met Leu
465                 470                 475                 480 
Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Xaa Tyr Gly
                485                 490                 495     
Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu
            500                 505                 510         
Ser Val Thr Ala Trp Gly Arg Xaa Tyr Ile Glu Phe Val Trp Lys Glu
        515                 520                 525             
Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly
    530                 535                 540                 
Leu Tyr Ala Thr Ile Pro Gly Gly Xaa Xaa Glu Glu Ile Lys Lys Lys
545                 550                 555                 560 
Ala Leu Glu Phe Val Xaa Tyr Ile Asn Xaa Lys Leu Pro Gly Leu Leu
                565                 570                 575     
Glu Leu Glu Tyr Glu Gly Phe Tyr Xaa Arg Gly Phe Phe Val Thr Lys
            580                 585                 590         
Lys Xaa Tyr Ala Xaa Ile Asp Glu Glu Gly Lys Xaa Ile Thr Arg Gly
        595                 600                 605             
Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln
    610                 615                 620                 
Ala Arg Val Leu Glu Xaa Ile Leu Lys His Gly Asn Val Glu Glu Ala
625                 630                 635                 640 
Val Xaa Ile Val Lys Glu Val Thr Gln Lys Leu Xaa Xaa Tyr Glu Ile
                645                 650                 655     
Pro Pro Glu Lys Leu Ala Ile Tyr Glu Gln Ile Thr Arg Pro Leu His
            660                 665                 670         
Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Xaa Leu Ala
        675                 680                 685             
Ala Xaa Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val
    690                 695                 700                 
Leu Arg Gly Asp Gly Pro Ile Ser Xaa Arg Ala Ile Leu Ala Glu Glu
705                 710                 715                 720 
Xaa Asp Pro Arg Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn
                725                 730                 735     
Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Gly Phe Gly Tyr Arg
            740                 745                 750         
Lys Glu Asp Leu Arg Trp Gln Lys Thr Lys Gln Xaa Gly Leu Thr Ser
        755                 760                 765             
Trp Leu Asn Ile Lys Lys Ser
    770                 775 



