                         SEQUENCE LISTING

<110>  Arizona Board of Regents for and on behalf of Arizona State
        University
       Krajmalnik-Brown, Rosa
       Halden, Rolf  U.
       Wilson, James W
 
<120>  METHODS AND SYSTEMS FOR TRACKING BIOREMEDIATION PROCESSES

<130>  60264WO

<150>  61/501,020
<151>  2011-06-24

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  2018
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Related sequences: J01641 and M10196 (bases 1-1300); M77749 
       (bases 1357-2296; 4537-4613); SYNPBR322 (bases 2297-2780); Gene 
       27, 183, 1984 (bases 2780-3238).

<400>  1
tgggctagcg aattcaggag gacagctatg agtgaaaaat atcattcaac tgttacccgg       60

cgtgatttta tgaaacgttt agggctggcc ggggccggcg ctggcgcact gggcgcggca      120

gtgcttgcgg aaaacaactt accgcatgaa tttaaggatg tcgatgatct gctgtcagcc      180

ggtaaagcct tggagggtga tcacgctaat aaagtgaaca accatccgtg gtgggtgacc      240

acccgtgatc atgaagatcc gacgtgcaac atcgattggt cgctgattaa acgttactcg      300

ggttggaaca accaaggtgc ctatttcctg ccggaagatt acttgagtcc aacttatacc      360

ggcagacgtc atacgattgt ggatagcaaa ttagaaattg aattacaggg taagaagtac      420

cgtgattccg cgtttattaa aagtggtatc gactggatga aagagaacat tgacccggat      480

tatgatccag gggaactggg ctacggcgat cgccgtgaag atgctcttat ctacgccgct      540

actaacggtt cccacaattg ttgggaaaac cctctttatg gccgctatga aggtagccgt      600

ccgtacctga gtatgcgtac catgaacggc attaatggcc tccatgaatt tggtcatgca      660

gacatcaaga cgactaacta cccgaaatgg gagggtacgc cggaagaaaa ccttctgata      720

atgcggaccg cggcccgcta ttttggcgcc tcatccgtcg gtgctatcaa gataaccgat      780

aacgtgaaaa agattttcta tgccaaagtg caaccgtttt gtctgggccc gtggtacacc      840

atcaccaaca tggccgaata tattgaatat ccggtgccgg ttgacaatta cgccattccg      900

attgtgtttg aagacattcc ggccgatcaa ggtcattatt cgtataagcg cttcggtggt      960

gatgataaaa ttgctgtgcc gaacgccctt gataacatat tcacgtacac gatcatgctg     1020

ccagaaaaac gttttaaata cgcgcattcg atcccgatgg atccgtgttc ttgcattgct     1080

tacccgctgt tcacagaagt tgaagctagg attcagcaat ttattgcggg cctgggttat     1140

aattccatgg gtggcggggt tgaagcgtgg gggccgggca gcgcttttgg aaacctatcg     1200

gggctggggg aacagtcgcg tgtcagcagc atcatcgagc cgcgttatgg atcaaatacc     1260

aaaggttcac tgcggatgct gacagacctg ccgctcgcac cgactaagcc aatcgacgcg     1320

ggcatccgcg agttctgtaa aacttgcggc atctgcgcgg aacactgccc gactcaagca     1380

atctctcacg aaggcccgcg gtatgactct ccccattggg attgtgttag cggttacgaa     1440

ggttggcact tagattatca taaatgtatt aactgcacca tctgtgaagc tgtttgccca     1500

ttctttacca tgagtaataa tagctgggtt cataatctgg tgaaaagcac cgtcgccact     1560

acgccggttt ttaatggctt tttcaaaaat atggaaggcg cgtttggcta tggtccccga     1620

tatagtccga gccgtgatga gtggtgggct agtgaaaatc caattcgggg cgcgagcgtt     1680

gatatctttt aagagaaagg atggaataga ttattatggg cggggctctg tattatttcc     1740

tggttggaat gctgattggt ggtgccgcca tttggtttat tacctatacc cagtttaaaa     1800

atattagctt taagtggtgg gaatggtctc tgatggcact gtcactgctg ctggtcagta     1860

gcatctttca acacatgtat tcttcaatga gcgtggaaat ggaatatcag agcgcattta     1920

tgtatctggg cgtgtttggt actctggccg tgatcctaaa cctgatcgtg tggcgtacgt     1980

atagcggcag aaaagaataa aatggtaccg catgcaac                             2018


<210>  2
<211>  4613
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Related sequences: J01641 and M10196 (bases 1-1300); M77749 
       (bases 1357-2296; 4537-4613); SYNPBR322 (bases 2297-2780); Gene 
       27, 183, 1984 (bases 2780-3238).


<220>
<221>  source
<222>  (1)..(4613)
<223>  /organism="synthetic construct" /mol_type="other DNA" 
       /db_xref="taxon:32630" /lab_host="Escherichia coli" 
       /plasmid="pBAD24"

<220>
<221>  mRNA
<222>  (96)..(974)
<223>  /gene="araC"

<220>
<221>  gene
<222>  (96)..(974)
<223>  /gene="araC"

<220>
<221>  misc_feature
<222>  (1003)..(1020)
<223>  /note="operator O2"

<220>
<221>  promoter
<222>  (1125)..(1153)
<223>  /note="Pc promoter"

<220>
<221>  misc_feature
<222>  (1161)..(1182)
<223>  /note="operator O1"

<220>
<221>  misc_feature
<222>  (1204)..(1217)
<223>  /note="CAP site"

<220>
<221>  misc_feature
<222>  (1213)..(1251)
<223>  /note="operator I2 + I1"

<220>
<221>  promoter
<222>  (1250)..(1277)
<223>  /note="PBAD promoter"

<220>
<221>  misc_feature
<222>  (1300)..(1362)
<223>  /note="multiple cloning site"

<220>
<221>  gene
<222>  (1363)..(1788)
<223>  /gene="rrnB"

<220>
<221>  terminator
<222>  (1363)..(1788)
<223>  /gene="rrnB"

<220>
<221>  promoter
<222>  (1843)..(1849)
<223>  /note="bla P3 promoter"

<220>
<221>  gene
<222>  (1881)..(2744)
<223>  /gene="bla"

<220>
<221>  mRNA
<222>  (1881)..(2744)
<223>  /gene="bla"

<220>
<221>  rep_origin
<222>  (2780)..(3238)
<223>  /note="M13 origin"

<220>
<221>  rep_origin
<222>  (3244)..(3941)
<223>  /note="pBR322 origin"

<300>
<301>  Guzman,L.M., Belin,D., Carson,M.J. and Beckwith,J.
<302>  Tight regulation, modulation, and high-level expression by 
       Vectors containing the arabinose PBAD promoter
<303>  J. Bacteriol.
<304>  177
<305>  14
<306>  4121-4130
<307>  1995-07   
<308>  X81838
<309>  1995-10-25

<400>  2
atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac       60

tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca      120

ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta      180

aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata      240

ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag      300

cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag      360

caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg      420

tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct      480

tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc      540

ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc      600

gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca      660

tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga      720

tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa      780

acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata      840

taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc      900

ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt      960

tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat     1020

tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta     1080

accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt     1140

aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca     1200

ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta     1260

tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt     1320

acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga     1380

gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag     1440

aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg     1500

aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag     1560

gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt     1620

gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa     1680

gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa     1740

gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt     1800

tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat     1860

aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt     1920

ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg     1980

ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga     2040

tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc     2100

tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac     2160

actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg     2220

gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca     2280

acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg     2340

gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg     2400

acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg     2460

gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag     2520

ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg     2580

gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct     2640

cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac     2700

agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact     2760

catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg     2820

gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct     2880

ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg     2940

ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg     3000

ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg     3060

gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact caaccctatc     3120

tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat     3180

gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa     3240

aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt     3300

ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt     3360

ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg     3420

tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca     3480

gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt     3540

agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga     3600

taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc     3660

gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact     3720

gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga     3780

caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg     3840

aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt     3900

tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt     3960

acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga     4020

ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac     4080

gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct     4140

ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc     4200

tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct     4260

gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca     4320

tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg     4380

tcatcaccga aacgcgcgag gcagcaagga gatggcgccc aacagtcccc cggccacggg     4440

gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc     4500

ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat     4560

gccggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt atc            4613


