
                                SEQUENCE LISTING

<110> Cox, Kevin
      Nguyen, Long

<120> COMPOSITIONS AND METHODS FOR MAKING AND
  BIOCONTAINING AUXOTROPHIC TRANSGENIC PLANTS
  

<130> 40989/420183

<150> 61/496,864
<151> 2011-06-14

<160> 41

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 2088
<212> DNA
<213> Lemna minor

<220> 
<221> 5'UTR          
<222> (1)...(40)
<223> 5'UTR of threonine deaminase (TD) isoform #1

<220> 
<221> CDS            
<222> (41)...(1999)
<223> Encodes threonine deaminase (TD)  isoform #1

<220> 
<221> 3'UTR          
<222> (2000)...(2008)
<223> 3'UTR of threonine deaminase (TD) isoform #1

<400> 1
ctctcggatc ctgcatcgtc ttcctcgtcc ctcgatcctc atg gcg gcg ctg cag   55
                                            Met Ala Ala Leu Gln
                                              1               5
 
atc ctt ccc cgg cca cag gcg cct tgt tcc ggc cga tct cca gcg cct   103
Ile Leu Pro Arg Pro Gln Ala Pro Cys Ser Gly Arg Ser Pro Ala Pro
                 10                  15                  20
 
tct ccg gct tct tcc gcc gcc act tgc tgc aca atg tcc aga tcc cca   151
Ser Pro Ala Ser Ser Ala Ala Thr Cys Cys Thr Met Ser Arg Ser Pro
             25                  30                  35
 
tcc ata tcc tta aag cgg tgt tct tgc tat cga tat ccc tct cgt tac   199
Ser Ile Ser Leu Lys Arg Cys Ser Cys Tyr Arg Tyr Pro Ser Arg Tyr
         40                  45                  50
 
tcc cat ggc atc ccc agt gat ggc gga atc aga ggc aaa ttg acc tca   247
Ser His Gly Ile Pro Ser Asp Gly Gly Ile Arg Gly Lys Leu Thr Ser
     55                  60                  65
 
tct gct gtt ccc gcc gca tca ttt gct tct cct tcc acc acc gcc gac   295
Ser Ala Val Pro Ala Ala Ser Phe Ala Ser Pro Ser Thr Thr Ala Asp
 70                  75                  80                  85
 
gcc cct agc gat gcc gca aca gct cca ttg tcg acc cca tcc gtc tct   343
Ala Pro Ser Asp Ala Ala Thr Ala Pro Leu Ser Thr Pro Ser Val Ser
                 90                  95                 100
 
tct gag gcc tcc gcc gaa gtt gaa ttg atg aag gtc acc acc gac tcg   391
Ser Glu Ala Ser Ala Glu Val Glu Leu Met Lys Val Thr Thr Asp Ser
            105                 110                 115
 
ctt cag tat gag agt ggg tat ctc ggg ggc att tcc gga aaa act cgt   439
Leu Gln Tyr Glu Ser Gly Tyr Leu Gly Gly Ile Ser Gly Lys Thr Arg
        120                 125                 130
 
ccc tct tgg ggg acg agc tgg acg agc agt cca tcg agc ttc gac agg   487
Pro Ser Trp Gly Thr Ser Trp Thr Ser Ser Pro Ser Ser Phe Asp Arg
    135                 140                 145
 
ccg agc gcc atg gat tac tta gct cac act ctc acc tcc aga gtc tac   535
Pro Ser Ala Met Asp Tyr Leu Ala His Thr Leu Thr Ser Arg Val Tyr
150                 155                 160                 165
 
gat gtg gcc atc gaa tcc ccc ctc cag ctc gct ccc agg ctt tcc gag   583
Asp Val Ala Ile Glu Ser Pro Leu Gln Leu Ala Pro Arg Leu Ser Glu
                170                 175                 180
 
cgg ctc ggt gtg cag ttc tgg ctg aag cgc gaa gat ctg caa cca gtg   631
Arg Leu Gly Val Gln Phe Trp Leu Lys Arg Glu Asp Leu Gln Pro Val
            185                 190                 195
 
ttc tca ttc aaa ttg cga gga gcg tat aat atg atg gcg aat ctt cct   679
Phe Ser Phe Lys Leu Arg Gly Ala Tyr Asn Met Met Ala Asn Leu Pro
        200                 205                 210
 
aga gaa aag ctg gaa aaa gga gta ata tgt tct tca gca ggg aat cac   727
Arg Glu Lys Leu Glu Lys Gly Val Ile Cys Ser Ser Ala Gly Asn His
    215                 220                 225
 
gct caa gga gtt gct ctg gct gca cag aaa cta ggc tgc aat gca gtg   775
Ala Gln Gly Val Ala Leu Ala Ala Gln Lys Leu Gly Cys Asn Ala Val
230                 235                 240                 245
 
atc gtc atg ccc gtt act acg cca gaa atc aag tgg aaa tct gtt gaa   823
Ile Val Met Pro Val Thr Thr Pro Glu Ile Lys Trp Lys Ser Val Glu
                250                 255                 260
 
aaa ttg ggc gca act gtt gtt ctt gtg gga gat tct tac gat gaa gcg   871
Lys Leu Gly Ala Thr Val Val Leu Val Gly Asp Ser Tyr Asp Glu Ala
            265                 270                 275
 
caa tcg cat gcc aag aaa aga gca aaa tcg gag ggc cgc act ttc att   919
Gln Ser His Ala Lys Lys Arg Ala Lys Ser Glu Gly Arg Thr Phe Ile
        280                 285                 290
 
ccg cct ttc gat aac cct aac gtc ata atg ggc caa gga act gtt gga   967
Pro Pro Phe Asp Asn Pro Asn Val Ile Met Gly Gln Gly Thr Val Gly
    295                 300                 305
 
atg gag atc atc agg caa ttg aga ggc ccg att cat gcc atc ttt gta   1015
Met Glu Ile Ile Arg Gln Leu Arg Gly Pro Ile His Ala Ile Phe Val
310                 315                 320                 325
 
ccc gtt ggt ggt ggt ctg att gct gga att gca gct tat gtg aaa caa   1063
Pro Val Gly Gly Gly Leu Ile Ala Gly Ile Ala Ala Tyr Val Lys Gln
                330                 335                 340
 
gtc cgc cct gag gtg aag atc atc ggt gtg gaa cca tac gat gca aat   1111
Val Arg Pro Glu Val Lys Ile Ile Gly Val Glu Pro Tyr Asp Ala Asn
            345                 350                 355
 
gcc atg gcg tta tcg ttg cat cat ggg cag agg gtc atg ctc gag caa   1159
Ala Met Ala Leu Ser Leu His His Gly Gln Arg Val Met Leu Glu Gln
        360                 365                 370
 
gtg ggc ggt ttc gca gat ggt gtt gct gtt aaa gtc gtc ggc gaa gaa   1207
Val Gly Gly Phe Ala Asp Gly Val Ala Val Lys Val Val Gly Glu Glu
    375                 380                 385
 
act tat cgc cta tgc cga gaa cta gtt gat ggt att gtt ctt gtc agt   1255
Thr Tyr Arg Leu Cys Arg Glu Leu Val Asp Gly Ile Val Leu Val Ser
390                 395                 400                 405
 
cgc gat gca att tgt gca tct ata aag gac atg ttc gag gaa aag agg   1303
Arg Asp Ala Ile Cys Ala Ser Ile Lys Asp Met Phe Glu Glu Lys Arg
                410                 415                 420
 
agt atc ctc gag cca gcc ggt gca ctc tca ttg gcc ggt gca gaa gct   1351
Ser Ile Leu Glu Pro Ala Gly Ala Leu Ser Leu Ala Gly Ala Glu Ala
            425                 430                 435
 
tac tgc aaa tac tac ggt ctg aag ggg gaa tct gtg gta gcc atc aca   1399
Tyr Cys Lys Tyr Tyr Gly Leu Lys Gly Glu Ser Val Val Ala Ile Thr
        440                 445                 450
 
tcg ggc gca aac atg aac ttt gat cgg ttg cga ttg gtt acc gag ctt   1447
Ser Gly Ala Asn Met Asn Phe Asp Arg Leu Arg Leu Val Thr Glu Leu
    455                 460                 465
 
gct gat gtg ggc cgt aaa caa gaa gct gtt ctc gcc act tcc atg ccg   1495
Ala Asp Val Gly Arg Lys Gln Glu Ala Val Leu Ala Thr Ser Met Pro
470                 475                 480                 485
 
gaa gaa ccc gga agc ttc aaa aga ttc tgt cag ctg gtg ggc ccg gtg   1543
Glu Glu Pro Gly Ser Phe Lys Arg Phe Cys Gln Leu Val Gly Pro Val
                490                 495                 500
 
aat atc acc gag ttc aag tac cgg tac gat gct agc aag gag aag gct   1591
Asn Ile Thr Glu Phe Lys Tyr Arg Tyr Asp Ala Ser Lys Glu Lys Ala
            505                 510                 515
 
ctt gtt ctt tac agt gtt gga gtg cat act gct gcg gag ctt aag tct   1639
Leu Val Leu Tyr Ser Val Gly Val His Thr Ala Ala Glu Leu Lys Ser
        520                 525                 530
 
gtg gta ggc cgc atg ggg ttt gaa aat ttt gag act gtt gat ctt acg   1687
Val Val Gly Arg Met Gly Phe Glu Asn Phe Glu Thr Val Asp Leu Thr
    535                 540                 545
 
aat aat gac ttg gcc aaa gat cat ctt cgt cat ctg gtt ggg ggt cgg   1735
Asn Asn Asp Leu Ala Lys Asp His Leu Arg His Leu Val Gly Gly Arg
550                 555                 560                 565
 
aca aat gtg gag aat gag ctg ctg tgt aga ttc atc ttc ccg gag agg   1783
Thr Asn Val Glu Asn Glu Leu Leu Cys Arg Phe Ile Phe Pro Glu Arg
                570                 575                 580
 
cct ggc acc ctg atg aag ttc ctc gac tcc ttc agc ccg cgc tgg aac   1831
Pro Gly Thr Leu Met Lys Phe Leu Asp Ser Phe Ser Pro Arg Trp Asn
            585                 590                 595
 
atc agt ctc ttc cac tat cga tcc cag ggg gag gcc ggg gca aat gtt   1879
Ile Ser Leu Phe His Tyr Arg Ser Gln Gly Glu Ala Gly Ala Asn Val
        600                 605                 610
 
ctg gtt gga atc cag gta cct gga ggc gag atg gac gag ttc cgc gcc   1927
Leu Val Gly Ile Gln Val Pro Gly Gly Glu Met Asp Glu Phe Arg Ala
    615                 620                 625
 
atc gcc acc aac cta gac tat gat tat gcc cta gag atg tcc aac aag   1975
Ile Ala Thr Asn Leu Asp Tyr Asp Tyr Ala Leu Glu Met Ser Asn Lys
630                 635                 640                 645
 
gct tac cag ctc ctc atg cac tga accatgggcc taaccctaat ttattgcaga  2029
Ala Tyr Gln Leu Leu Met His  *
                650
 
tgatgatgat gataatgatg atgatgatag ttgtgttgta tgcggtgtta tggcttctg  2088

<210> 2
<211> 1959
<212> DNA
<213> Lemna minor

<220> 
<221> CDS            
<222> (1)...(1959)
<223> Encodes threonine deaminase (TD) isoform #1

<400> 2
atg gcg gcg ctg cag atc ctt ccc cgg cca cag gcg cct tgt tcc ggc   48
Met Ala Ala Leu Gln Ile Leu Pro Arg Pro Gln Ala Pro Cys Ser Gly
 1               5                   10                  15
 
cga tct cca gcg cct tct ccg gct tct tcc gcc gcc act tgc tgc aca   96
Arg Ser Pro Ala Pro Ser Pro Ala Ser Ser Ala Ala Thr Cys Cys Thr
             20                  25                  30
 
atg tcc aga tcc cca tcc ata tcc tta aag cgg tgt tct tgc tat cga   144
Met Ser Arg Ser Pro Ser Ile Ser Leu Lys Arg Cys Ser Cys Tyr Arg
         35                  40                  45
 
tat ccc tct cgt tac tcc cat ggc atc ccc agt gat ggc gga atc aga   192
Tyr Pro Ser Arg Tyr Ser His Gly Ile Pro Ser Asp Gly Gly Ile Arg
     50                  55                  60
 
ggc aaa ttg acc tca tct gct gtt ccc gcc gca tca ttt gct tct cct   240
Gly Lys Leu Thr Ser Ser Ala Val Pro Ala Ala Ser Phe Ala Ser Pro
 65                  70                  75                  80
 
tcc acc acc gcc gac gcc cct agc gat gcc gca aca gct cca ttg tcg   288
Ser Thr Thr Ala Asp Ala Pro Ser Asp Ala Ala Thr Ala Pro Leu Ser
                 85                  90                  95
 
acc cca tcc gtc tct tct gag gcc tcc gcc gaa gtt gaa ttg atg aag   336
Thr Pro Ser Val Ser Ser Glu Ala Ser Ala Glu Val Glu Leu Met Lys
            100                 105                 110
 
gtc acc acc gac tcg ctt cag tat gag agt ggg tat ctc ggg ggc att   384
Val Thr Thr Asp Ser Leu Gln Tyr Glu Ser Gly Tyr Leu Gly Gly Ile
        115                 120                 125
 
tcc gga aaa act cgt ccc tct tgg ggg acg agc tgg acg agc agt cca   432
Ser Gly Lys Thr Arg Pro Ser Trp Gly Thr Ser Trp Thr Ser Ser Pro
    130                 135                 140
 
tcg agc ttc gac agg ccg agc gcc atg gat tac tta gct cac act ctc   480
Ser Ser Phe Asp Arg Pro Ser Ala Met Asp Tyr Leu Ala His Thr Leu
145                 150                 155                 160
 
acc tcc aga gtc tac gat gtg gcc atc gaa tcc ccc ctc cag ctc gct   528
Thr Ser Arg Val Tyr Asp Val Ala Ile Glu Ser Pro Leu Gln Leu Ala
                165                 170                 175
 
ccc agg ctt tcc gag cgg ctc ggt gtg cag ttc tgg ctg aag cgc gaa   576
Pro Arg Leu Ser Glu Arg Leu Gly Val Gln Phe Trp Leu Lys Arg Glu
            180                 185                 190
 
gat ctg caa cca gtg ttc tca ttc aaa ttg cga gga gcg tat aat atg   624
Asp Leu Gln Pro Val Phe Ser Phe Lys Leu Arg Gly Ala Tyr Asn Met
        195                 200                 205
 
atg gcg aat ctt cct aga gaa aag ctg gaa aaa gga gta ata tgt tct   672
Met Ala Asn Leu Pro Arg Glu Lys Leu Glu Lys Gly Val Ile Cys Ser
    210                 215                 220
 
tca gca ggg aat cac gct caa gga gtt gct ctg gct gca cag aaa cta   720
Ser Ala Gly Asn His Ala Gln Gly Val Ala Leu Ala Ala Gln Lys Leu
225                 230                 235                 240
 
ggc tgc aat gca gtg atc gtc atg ccc gtt act acg cca gaa atc aag   768
Gly Cys Asn Ala Val Ile Val Met Pro Val Thr Thr Pro Glu Ile Lys
                245                 250                 255
 
tgg aaa tct gtt gaa aaa ttg ggc gca act gtt gtt ctt gtg gga gat   816
Trp Lys Ser Val Glu Lys Leu Gly Ala Thr Val Val Leu Val Gly Asp
            260                 265                 270
 
tct tac gat gaa gcg caa tcg cat gcc aag aaa aga gca aaa tcg gag   864
Ser Tyr Asp Glu Ala Gln Ser His Ala Lys Lys Arg Ala Lys Ser Glu
        275                 280                 285
 
ggc cgc act ttc att ccg cct ttc gat aac cct aac gtc ata atg ggc   912
Gly Arg Thr Phe Ile Pro Pro Phe Asp Asn Pro Asn Val Ile Met Gly
    290                 295                 300
 
caa gga act gtt gga atg gag atc atc agg caa ttg aga ggc ccg att   960
Gln Gly Thr Val Gly Met Glu Ile Ile Arg Gln Leu Arg Gly Pro Ile
305                 310                 315                 320
 
cat gcc atc ttt gta ccc gtt ggt ggt ggt ctg att gct gga att gca   1008
His Ala Ile Phe Val Pro Val Gly Gly Gly Leu Ile Ala Gly Ile Ala
                325                 330                 335
 
gct tat gtg aaa caa gtc cgc cct gag gtg aag atc atc ggt gtg gaa   1056
Ala Tyr Val Lys Gln Val Arg Pro Glu Val Lys Ile Ile Gly Val Glu
            340                 345                 350
 
cca tac gat gca aat gcc atg gcg tta tcg ttg cat cat ggg cag agg   1104
Pro Tyr Asp Ala Asn Ala Met Ala Leu Ser Leu His His Gly Gln Arg
        355                 360                 365
 
gtc atg ctc gag caa gtg ggc ggt ttc gca gat ggt gtt gct gtt aaa   1152
Val Met Leu Glu Gln Val Gly Gly Phe Ala Asp Gly Val Ala Val Lys
    370                 375                 380
 
gtc gtc ggc gaa gaa act tat cgc cta tgc cga gaa cta gtt gat ggt   1200
Val Val Gly Glu Glu Thr Tyr Arg Leu Cys Arg Glu Leu Val Asp Gly
385                 390                 395                 400
 
att gtt ctt gtc agt cgc gat gca att tgt gca tct ata aag gac atg   1248
Ile Val Leu Val Ser Arg Asp Ala Ile Cys Ala Ser Ile Lys Asp Met
                405                 410                 415
 
ttc gag gaa aag agg agt atc ctc gag cca gcc ggt gca ctc tca ttg   1296
Phe Glu Glu Lys Arg Ser Ile Leu Glu Pro Ala Gly Ala Leu Ser Leu
            420                 425                 430
 
gcc ggt gca gaa gct tac tgc aaa tac tac ggt ctg aag ggg gaa tct   1344
Ala Gly Ala Glu Ala Tyr Cys Lys Tyr Tyr Gly Leu Lys Gly Glu Ser
        435                 440                 445
 
gtg gta gcc atc aca tcg ggc gca aac atg aac ttt gat cgg ttg cga   1392
Val Val Ala Ile Thr Ser Gly Ala Asn Met Asn Phe Asp Arg Leu Arg
    450                 455                 460
 
ttg gtt acc gag ctt gct gat gtg ggc cgt aaa caa gaa gct gtt ctc   1440
Leu Val Thr Glu Leu Ala Asp Val Gly Arg Lys Gln Glu Ala Val Leu
465                 470                 475                 480
 
gcc act tcc atg ccg gaa gaa ccc gga agc ttc aaa aga ttc tgt cag   1488
Ala Thr Ser Met Pro Glu Glu Pro Gly Ser Phe Lys Arg Phe Cys Gln
                485                 490                 495
 
ctg gtg ggc ccg gtg aat atc acc gag ttc aag tac cgg tac gat gct   1536
Leu Val Gly Pro Val Asn Ile Thr Glu Phe Lys Tyr Arg Tyr Asp Ala
            500                 505                 510
 
agc aag gag aag gct ctt gtt ctt tac agt gtt gga gtg cat act gct   1584
Ser Lys Glu Lys Ala Leu Val Leu Tyr Ser Val Gly Val His Thr Ala
        515                 520                 525
 
gcg gag ctt aag tct gtg gta ggc cgc atg ggg ttt gaa aat ttt gag   1632
Ala Glu Leu Lys Ser Val Val Gly Arg Met Gly Phe Glu Asn Phe Glu
    530                 535                 540
 
act gtt gat ctt acg aat aat gac ttg gcc aaa gat cat ctt cgt cat   1680
Thr Val Asp Leu Thr Asn Asn Asp Leu Ala Lys Asp His Leu Arg His
545                 550                 555                 560
 
ctg gtt ggg ggt cgg aca aat gtg gag aat gag ctg ctg tgt aga ttc   1728
Leu Val Gly Gly Arg Thr Asn Val Glu Asn Glu Leu Leu Cys Arg Phe
                565                 570                 575
 
atc ttc ccg gag agg cct ggc acc ctg atg aag ttc ctc gac tcc ttc   1776
Ile Phe Pro Glu Arg Pro Gly Thr Leu Met Lys Phe Leu Asp Ser Phe
            580                 585                 590
 
agc ccg cgc tgg aac atc agt ctc ttc cac tat cga tcc cag ggg gag   1824
Ser Pro Arg Trp Asn Ile Ser Leu Phe His Tyr Arg Ser Gln Gly Glu
        595                 600                 605
 
gcc ggg gca aat gtt ctg gtt gga atc cag gta cct gga ggc gag atg   1872
Ala Gly Ala Asn Val Leu Val Gly Ile Gln Val Pro Gly Gly Glu Met
    610                 615                 620
 
gac gag ttc cgc gcc atc gcc acc aac cta gac tat gat tat gcc cta   1920
Asp Glu Phe Arg Ala Ile Ala Thr Asn Leu Asp Tyr Asp Tyr Ala Leu
625                 630                 635                 640
 
gag atg tcc aac aag gct tac cag ctc ctc atg cac tga               1959
Glu Met Ser Asn Lys Ala Tyr Gln Leu Leu Met His  *
                645                 650
 

<210> 3
<211> 652
<212> PRT
<213> Lemna minor

<400> 3
Met Ala Ala Leu Gln Ile Leu Pro Arg Pro Gln Ala Pro Cys Ser Gly
 1               5                  10                  15      
Arg Ser Pro Ala Pro Ser Pro Ala Ser Ser Ala Ala Thr Cys Cys Thr
            20                  25                  30          
Met Ser Arg Ser Pro Ser Ile Ser Leu Lys Arg Cys Ser Cys Tyr Arg
        35                  40                  45              
Tyr Pro Ser Arg Tyr Ser His Gly Ile Pro Ser Asp Gly Gly Ile Arg
    50                  55                  60                  
Gly Lys Leu Thr Ser Ser Ala Val Pro Ala Ala Ser Phe Ala Ser Pro
65                  70                  75                  80  
Ser Thr Thr Ala Asp Ala Pro Ser Asp Ala Ala Thr Ala Pro Leu Ser
                85                  90                  95      
Thr Pro Ser Val Ser Ser Glu Ala Ser Ala Glu Val Glu Leu Met Lys
            100                 105                 110         
Val Thr Thr Asp Ser Leu Gln Tyr Glu Ser Gly Tyr Leu Gly Gly Ile
        115                 120                 125             
Ser Gly Lys Thr Arg Pro Ser Trp Gly Thr Ser Trp Thr Ser Ser Pro
    130                 135                 140                 
Ser Ser Phe Asp Arg Pro Ser Ala Met Asp Tyr Leu Ala His Thr Leu
145                 150                 155                 160 
Thr Ser Arg Val Tyr Asp Val Ala Ile Glu Ser Pro Leu Gln Leu Ala
                165                 170                 175     
Pro Arg Leu Ser Glu Arg Leu Gly Val Gln Phe Trp Leu Lys Arg Glu
            180                 185                 190         
Asp Leu Gln Pro Val Phe Ser Phe Lys Leu Arg Gly Ala Tyr Asn Met
        195                 200                 205             
Met Ala Asn Leu Pro Arg Glu Lys Leu Glu Lys Gly Val Ile Cys Ser
    210                 215                 220                 
Ser Ala Gly Asn His Ala Gln Gly Val Ala Leu Ala Ala Gln Lys Leu
225                 230                 235                 240 
Gly Cys Asn Ala Val Ile Val Met Pro Val Thr Thr Pro Glu Ile Lys
                245                 250                 255     
Trp Lys Ser Val Glu Lys Leu Gly Ala Thr Val Val Leu Val Gly Asp
            260                 265                 270         
Ser Tyr Asp Glu Ala Gln Ser His Ala Lys Lys Arg Ala Lys Ser Glu
        275                 280                 285             
Gly Arg Thr Phe Ile Pro Pro Phe Asp Asn Pro Asn Val Ile Met Gly
    290                 295                 300                 
Gln Gly Thr Val Gly Met Glu Ile Ile Arg Gln Leu Arg Gly Pro Ile
305                 310                 315                 320 
His Ala Ile Phe Val Pro Val Gly Gly Gly Leu Ile Ala Gly Ile Ala
                325                 330                 335     
Ala Tyr Val Lys Gln Val Arg Pro Glu Val Lys Ile Ile Gly Val Glu
            340                 345                 350         
Pro Tyr Asp Ala Asn Ala Met Ala Leu Ser Leu His His Gly Gln Arg
        355                 360                 365             
Val Met Leu Glu Gln Val Gly Gly Phe Ala Asp Gly Val Ala Val Lys
    370                 375                 380                 
Val Val Gly Glu Glu Thr Tyr Arg Leu Cys Arg Glu Leu Val Asp Gly
385                 390                 395                 400 
Ile Val Leu Val Ser Arg Asp Ala Ile Cys Ala Ser Ile Lys Asp Met
                405                 410                 415     
Phe Glu Glu Lys Arg Ser Ile Leu Glu Pro Ala Gly Ala Leu Ser Leu
            420                 425                 430         
Ala Gly Ala Glu Ala Tyr Cys Lys Tyr Tyr Gly Leu Lys Gly Glu Ser
        435                 440                 445             
Val Val Ala Ile Thr Ser Gly Ala Asn Met Asn Phe Asp Arg Leu Arg
    450                 455                 460                 
Leu Val Thr Glu Leu Ala Asp Val Gly Arg Lys Gln Glu Ala Val Leu
465                 470                 475                 480 
Ala Thr Ser Met Pro Glu Glu Pro Gly Ser Phe Lys Arg Phe Cys Gln
                485                 490                 495     
Leu Val Gly Pro Val Asn Ile Thr Glu Phe Lys Tyr Arg Tyr Asp Ala
            500                 505                 510         
Ser Lys Glu Lys Ala Leu Val Leu Tyr Ser Val Gly Val His Thr Ala
        515                 520                 525             
Ala Glu Leu Lys Ser Val Val Gly Arg Met Gly Phe Glu Asn Phe Glu
    530                 535                 540                 
Thr Val Asp Leu Thr Asn Asn Asp Leu Ala Lys Asp His Leu Arg His
545                 550                 555                 560 
Leu Val Gly Gly Arg Thr Asn Val Glu Asn Glu Leu Leu Cys Arg Phe
                565                 570                 575     
Ile Phe Pro Glu Arg Pro Gly Thr Leu Met Lys Phe Leu Asp Ser Phe
            580                 585                 590         
Ser Pro Arg Trp Asn Ile Ser Leu Phe His Tyr Arg Ser Gln Gly Glu
        595                 600                 605             
Ala Gly Ala Asn Val Leu Val Gly Ile Gln Val Pro Gly Gly Glu Met
    610                 615                 620                 
Asp Glu Phe Arg Ala Ile Ala Thr Asn Leu Asp Tyr Asp Tyr Ala Leu
625                 630                 635                 640 
Glu Met Ser Asn Lys Ala Tyr Gln Leu Leu Met His
                645                 650         


<210> 4
<211> 2091
<212> DNA
<213> Lemna minor

<220> 
<221> 5'UTR          
<222> (1)...(40)
<223> 5'UTR for threonine deaminase (TD) isoform #2

<220> 
<221> CDS            
<222> (41)...(1447)
<223> Encodes threonine deaminase (TD) isoform #2

<220> 
<221> 5'UTR          
<222> (1448)...(2091)
<223> 3'UTR for threonine deaminase (TD) isoform #2

<400> 4
ctctcggatc ctgcatcgtc ttcctcgtcc ctcgatcctc atg gcg gcg ctg cag   55
                                             Met Ala Ala Leu Gln
                                              1               5
 
atc ctt ccc cgg cca cag gcg cct tgt tcc ggc cga tct cca gcg cct   103
Ile Leu Pro Arg Pro Gln Ala Pro Cys Ser Gly Arg Ser Pro Ala Pro
                 10                  15                  20
 
tct ccg gct tct tcc gcc gcc act tgc tgc aca atg tcc aga tcc cca   151
Ser Pro Ala Ser Ser Ala Ala Thr Cys Cys Thr Met Ser Arg Ser Pro
             25                  30                  35
 
tcc ata tcc tta aag cgg tgt tct tgc tat cga tat ccc tct cgt tac   199
Ser Ile Ser Leu Lys Arg Cys Ser Cys Tyr Arg Tyr Pro Ser Arg Tyr
         40                  45                  50
 
tcc cat ggc atc ccc agt gat ggc gga atc aga ggc aaa ttg acc tca   247
Ser His Gly Ile Pro Ser Asp Gly Gly Ile Arg Gly Lys Leu Thr Ser
     55                  60                  65
 
tct gct gtt tcc gcc gca tca ttt gct tct cct tcc acc acc gcc gac   295
Ser Ala Val Ser Ala Ala Ser Phe Ala Ser Pro Ser Thr Thr Ala Asp
 70                  75                  80                  85
 
gcc cct agc gat gcc gca aca gct cca ttg tcg acc cca tcc gtc tct   343
Ala Pro Ser Asp Ala Ala Thr Ala Pro Leu Ser Thr Pro Ser Val Ser
                 90                  95                 100
 
tct gag gcc tcc gcc gaa gtt gaa ttg atg aag gtc acc acc gac tcg   391
Ser Glu Ala Ser Ala Glu Val Glu Leu Met Lys Val Thr Thr Asp Ser
            105                 110                 115
 
ctt cag tat gag agt ggg tat ctc ggg ggc att tcc gga aaa act cgt   439
Leu Gln Tyr Glu Ser Gly Tyr Leu Gly Gly Ile Ser Gly Lys Thr Arg
        120                 125                 130
 
ccc tct tgg ggg acg agc tgg acg agc agt cca tcg agc ttc gac agg   487
Pro Ser Trp Gly Thr Ser Trp Thr Ser Ser Pro Ser Ser Phe Asp Arg
    135                 140                 145
 
ccg agc gcc atg gat tac tta gct cac act ctc acc tcc aga gtc tac   535
Pro Ser Ala Met Asp Tyr Leu Ala His Thr Leu Thr Ser Arg Val Tyr
150                 155                 160                 165
 
gat gtg gcc atc gaa tcc ccc ctc cag ctc gct ccc agg ctt tcc gag   583
Asp Val Ala Ile Glu Ser Pro Leu Gln Leu Ala Pro Arg Leu Ser Glu
                170                 175                 180
 
cgg ctc ggt gtg cag ttc tgg ctg aag cgc gaa gat ctg caa cca gtg   631
Arg Leu Gly Val Gln Phe Trp Leu Lys Arg Glu Asp Leu Gln Pro Val
            185                 190                 195
 
ttc tca ttc aaa ttg cga gga gcg tat aat atg atg gcg aat ctt cct   679
Phe Ser Phe Lys Leu Arg Gly Ala Tyr Asn Met Met Ala Asn Leu Pro
        200                 205                 210
 
aga gaa aag ctg gaa aaa gga gta ata tgt tct tca gca ggg aat cac   727
Arg Glu Lys Leu Glu Lys Gly Val Ile Cys Ser Ser Ala Gly Asn His
    215                 220                 225
 
gct caa gga gtt gct ctg gct gca cag aaa cta ggc tgc aat gca gtg   775
Ala Gln Gly Val Ala Leu Ala Ala Gln Lys Leu Gly Cys Asn Ala Val
230                 235                 240                 245
 
atc gtc atg ccc gtt act acg cca gaa atc aag tgg aaa tct gtt gaa   823
Ile Val Met Pro Val Thr Thr Pro Glu Ile Lys Trp Lys Ser Val Glu
                250                 255                 260
 
aaa ttg ggc gca act gtt gtt ctt gtg gga gat tct tac gat gaa gcg   871
Lys Leu Gly Ala Thr Val Val Leu Val Gly Asp Ser Tyr Asp Glu Ala
            265                 270                 275
 
caa tcg cat gcc aag aaa aga gca aaa tcg gag ggc cgc act ttc att   919
Gln Ser His Ala Lys Lys Arg Ala Lys Ser Glu Gly Arg Thr Phe Ile
        280                 285                 290
 
ccg cct ttc gat aac cct aac gtc ata atg ggc caa gga act gtt gga   967
Pro Pro Phe Asp Asn Pro Asn Val Ile Met Gly Gln Gly Thr Val Gly
    295                 300                 305
 
atg gag atc atc agg caa ttg aga ggc ccg att cat gcc atc ttt gta   1015
Met Glu Ile Ile Arg Gln Leu Arg Gly Pro Ile His Ala Ile Phe Val
310                 315                 320                 325
 
ccc gtt ggt ggt ggt ggt ctg att gct gga att gca gct tat gtg aaa   1063
Pro Val Gly Gly Gly Gly Leu Ile Ala Gly Ile Ala Ala Tyr Val Lys
                330                 335                 340
 
caa gtc cgc cct gag gtg aag atc atc ggt gtg gaa cca tac gat gca   1111
Gln Val Arg Pro Glu Val Lys Ile Ile Gly Val Glu Pro Tyr Asp Ala
            345                 350                 355
 
aat gcc atg gcg tta tcg ttg cat cat ggg cag agg gtc atg ctc gag   1159
Asn Ala Met Ala Leu Ser Leu His His Gly Gln Arg Val Met Leu Glu
        360                 365                 370
 
caa gtg ggc ggt ttc gca gat ggt gtt gct gtt aaa gtc gtc ggc gaa   1207
Gln Val Gly Gly Phe Ala Asp Gly Val Ala Val Lys Val Val Gly Glu
    375                 380                 385
 
gaa act tat cgc cta tgc cga gaa cta gtt gat ggt att gtt ctt gtc   1255
Glu Thr Tyr Arg Leu Cys Arg Glu Leu Val Asp Gly Ile Val Leu Val
390                 395                 400                 405
 
agt cgc gat gca att tgt gca tct ata aag gac atg ttc gag gaa aag   1303
Ser Arg Asp Ala Ile Cys Ala Ser Ile Lys Asp Met Phe Glu Glu Lys
                410                 415                 420
 
agg agt atc ctc gag cca gcc ggt gca ctc tca ttg gcc ggt gca gaa   1351
Arg Ser Ile Leu Glu Pro Ala Gly Ala Leu Ser Leu Ala Gly Ala Glu
            425                 430                 435
 
gct tac tgc aaa tac tac ggt ctg aag ggg gaa tct gtg gta gcc atc   1399
Ala Tyr Cys Lys Tyr Tyr Gly Leu Lys Gly Glu Ser Val Val Ala Ile
        440                 445                 450
 
aca tcg ggc gca aac atg aac ttt gat cgg ttg cga ttg gtt acc tag   1447
Thr Ser Gly Ala Asn Met Asn Phe Asp Arg Leu Arg Leu Val Thr  
    455                 460                 465
 
cttgctgatg tgggccgtaa acaagaagct gttctcgcca cttccatgcc ggaagaaccc 1507
ggaagcttca aaagattctg tcagctggtg ggcccggtga atatcaccga gttcaagtac 1567
cggtacgatg ctagcaagga gaaggctctt gttctttaca gtgttggagt gcatactgct 1627
gcggagctta agtctatggt aggccgcatg gagtttgaaa attttgagac tgttgatctt 1687
acgaataatg acttggccaa agatcatctt cgtcatctgg ttgggggtcg gacaaatgtg 1747
gagaatgagc tgctgtgtag attcatcttc ccggagaggc ctggcaccct gatgaagttc 1807
ctcgactcct tcagcccgcg ctggaacatc agtctcttcc actatcgatc ccagggggag 1867
gccggggcaa atgttctggt tggaatccag gtacctggag gcgagatgga cgagttccgc 1927
gccatcgcca ccaacctaga ctatgattat gccctagaga tgtccaacaa ggcttaccag 1987
ctcctcatgc actgaaccat gggcctaacc ctaatttatt gcagatgatg atgatgataa 2047
tgatgatgat gatagttgtg ttgtatgcgg tgttatggct tctg                  2091

<210> 5
<211> 1407
<212> DNA
<213> Lemna minor

<220> 
<221> CDS            
<222> (1)...(1407)
<223> Encodes threonine deaminase (TD) isoform #2

<400> 5
atg gcg gcg ctg cag atc ctt ccc cgg cca cag gcg cct tgt tcc ggc   48
Met Ala Ala Leu Gln Ile Leu Pro Arg Pro Gln Ala Pro Cys Ser Gly
 1               5                   10                  15
 
cga tct cca gcg cct tct ccg gct tct tcc gcc gcc act tgc tgc aca   96
Arg Ser Pro Ala Pro Ser Pro Ala Ser Ser Ala Ala Thr Cys Cys Thr
             20                  25                  30
 
atg tcc aga tcc cca tcc ata tcc tta aag cgg tgt tct tgc tat cga   144
Met Ser Arg Ser Pro Ser Ile Ser Leu Lys Arg Cys Ser Cys Tyr Arg
         35                  40                  45
 
tat ccc tct cgt tac tcc cat ggc atc ccc agt gat ggc gga atc aga   192
Tyr Pro Ser Arg Tyr Ser His Gly Ile Pro Ser Asp Gly Gly Ile Arg
     50                  55                  60
 
ggc aaa ttg acc tca tct gct gtt tcc gcc gca tca ttt gct tct cct   240
Gly Lys Leu Thr Ser Ser Ala Val Ser Ala Ala Ser Phe Ala Ser Pro
 65                  70                  75                  80
 
tcc acc acc gcc gac gcc cct agc gat gcc gca aca gct cca ttg tcg   288
Ser Thr Thr Ala Asp Ala Pro Ser Asp Ala Ala Thr Ala Pro Leu Ser
                 85                  90                  95
 
acc cca tcc gtc tct tct gag gcc tcc gcc gaa gtt gaa ttg atg aag   336
Thr Pro Ser Val Ser Ser Glu Ala Ser Ala Glu Val Glu Leu Met Lys
            100                 105                 110
 
gtc acc acc gac tcg ctt cag tat gag agt ggg tat ctc ggg ggc att   384
Val Thr Thr Asp Ser Leu Gln Tyr Glu Ser Gly Tyr Leu Gly Gly Ile
        115                 120                 125
 
tcc gga aaa act cgt ccc tct tgg ggg acg agc tgg acg agc agt cca   432
Ser Gly Lys Thr Arg Pro Ser Trp Gly Thr Ser Trp Thr Ser Ser Pro
    130                 135                 140
 
tcg agc ttc gac agg ccg agc gcc atg gat tac tta gct cac act ctc   480
Ser Ser Phe Asp Arg Pro Ser Ala Met Asp Tyr Leu Ala His Thr Leu
145                 150                 155                 160
 
acc tcc aga gtc tac gat gtg gcc atc gaa tcc ccc ctc cag ctc gct   528
Thr Ser Arg Val Tyr Asp Val Ala Ile Glu Ser Pro Leu Gln Leu Ala
                165                 170                 175
 
ccc agg ctt tcc gag cgg ctc ggt gtg cag ttc tgg ctg aag cgc gaa   576
Pro Arg Leu Ser Glu Arg Leu Gly Val Gln Phe Trp Leu Lys Arg Glu
            180                 185                 190
 
gat ctg caa cca gtg ttc tca ttc aaa ttg cga gga gcg tat aat atg   624
Asp Leu Gln Pro Val Phe Ser Phe Lys Leu Arg Gly Ala Tyr Asn Met
        195                 200                 205
 
atg gcg aat ctt cct aga gaa aag ctg gaa aaa gga gta ata tgt tct   672
Met Ala Asn Leu Pro Arg Glu Lys Leu Glu Lys Gly Val Ile Cys Ser
    210                 215                 220
 
tca gca ggg aat cac gct caa gga gtt gct ctg gct gca cag aaa cta   720
Ser Ala Gly Asn His Ala Gln Gly Val Ala Leu Ala Ala Gln Lys Leu
225                 230                 235                 240
 
ggc tgc aat gca gtg atc gtc atg ccc gtt act acg cca gaa atc aag   768
Gly Cys Asn Ala Val Ile Val Met Pro Val Thr Thr Pro Glu Ile Lys
                245                 250                 255
 
tgg aaa tct gtt gaa aaa ttg ggc gca act gtt gtt ctt gtg gga gat   816
Trp Lys Ser Val Glu Lys Leu Gly Ala Thr Val Val Leu Val Gly Asp
            260                 265                 270
 
tct tac gat gaa gcg caa tcg cat gcc aag aaa aga gca aaa tcg gag   864
Ser Tyr Asp Glu Ala Gln Ser His Ala Lys Lys Arg Ala Lys Ser Glu
        275                 280                 285
 
ggc cgc act ttc att ccg cct ttc gat aac cct aac gtc ata atg ggc   912
Gly Arg Thr Phe Ile Pro Pro Phe Asp Asn Pro Asn Val Ile Met Gly
    290                 295                 300
 
caa gga act gtt gga atg gag atc atc agg caa ttg aga ggc ccg att   960
Gln Gly Thr Val Gly Met Glu Ile Ile Arg Gln Leu Arg Gly Pro Ile
305                 310                 315                 320
 
cat gcc atc ttt gta ccc gtt ggt ggt ggt ggt ctg att gct gga att   1008
His Ala Ile Phe Val Pro Val Gly Gly Gly Gly Leu Ile Ala Gly Ile
                325                 330                 335
 
gca gct tat gtg aaa caa gtc cgc cct gag gtg aag atc atc ggt gtg   1056
Ala Ala Tyr Val Lys Gln Val Arg Pro Glu Val Lys Ile Ile Gly Val
            340                 345                 350
 
gaa cca tac gat gca aat gcc atg gcg tta tcg ttg cat cat ggg cag   1104
Glu Pro Tyr Asp Ala Asn Ala Met Ala Leu Ser Leu His His Gly Gln
        355                 360                 365
 
agg gtc atg ctc gag caa gtg ggc ggt ttc gca gat ggt gtt gct gtt   1152
Arg Val Met Leu Glu Gln Val Gly Gly Phe Ala Asp Gly Val Ala Val
    370                 375                 380
 
aaa gtc gtc ggc gaa gaa act tat cgc cta tgc cga gaa cta gtt gat   1200
Lys Val Val Gly Glu Glu Thr Tyr Arg Leu Cys Arg Glu Leu Val Asp
385                 390                 395                 400
 
ggt att gtt ctt gtc agt cgc gat gca att tgt gca tct ata aag gac   1248
Gly Ile Val Leu Val Ser Arg Asp Ala Ile Cys Ala Ser Ile Lys Asp
                405                 410                 415
 
atg ttc gag gaa aag agg agt atc ctc gag cca gcc ggt gca ctc tca   1296
Met Phe Glu Glu Lys Arg Ser Ile Leu Glu Pro Ala Gly Ala Leu Ser
            420                 425                 430
 
ttg gcc ggt gca gaa gct tac tgc aaa tac tac ggt ctg aag ggg gaa   1344
Leu Ala Gly Ala Glu Ala Tyr Cys Lys Tyr Tyr Gly Leu Lys Gly Glu
        435                 440                 445
 
tct gtg gta gcc atc aca tcg ggc gca aac atg aac ttt gat cgg ttg   1392
Ser Val Val Ala Ile Thr Ser Gly Ala Asn Met Asn Phe Asp Arg Leu
    450                 455                 460
 
cga ttg gtt acc tag                                               1407
Arg Leu Val Thr  
465
 

<210> 6
<211> 468
<212> PRT
<213> Lemna minor

<400> 6
Met Ala Ala Leu Gln Ile Leu Pro Arg Pro Gln Ala Pro Cys Ser Gly
 1               5                  10                  15      
Arg Ser Pro Ala Pro Ser Pro Ala Ser Ser Ala Ala Thr Cys Cys Thr
            20                  25                  30          
Met Ser Arg Ser Pro Ser Ile Ser Leu Lys Arg Cys Ser Cys Tyr Arg
        35                  40                  45              
Tyr Pro Ser Arg Tyr Ser His Gly Ile Pro Ser Asp Gly Gly Ile Arg
    50                  55                  60                  
Gly Lys Leu Thr Ser Ser Ala Val Ser Ala Ala Ser Phe Ala Ser Pro
65                  70                  75                  80  
Ser Thr Thr Ala Asp Ala Pro Ser Asp Ala Ala Thr Ala Pro Leu Ser
                85                  90                  95      
Thr Pro Ser Val Ser Ser Glu Ala Ser Ala Glu Val Glu Leu Met Lys
            100                 105                 110         
Val Thr Thr Asp Ser Leu Gln Tyr Glu Ser Gly Tyr Leu Gly Gly Ile
        115                 120                 125             
Ser Gly Lys Thr Arg Pro Ser Trp Gly Thr Ser Trp Thr Ser Ser Pro
    130                 135                 140                 
Ser Ser Phe Asp Arg Pro Ser Ala Met Asp Tyr Leu Ala His Thr Leu
145                 150                 155                 160 
Thr Ser Arg Val Tyr Asp Val Ala Ile Glu Ser Pro Leu Gln Leu Ala
                165                 170                 175     
Pro Arg Leu Ser Glu Arg Leu Gly Val Gln Phe Trp Leu Lys Arg Glu
            180                 185                 190         
Asp Leu Gln Pro Val Phe Ser Phe Lys Leu Arg Gly Ala Tyr Asn Met
        195                 200                 205             
Met Ala Asn Leu Pro Arg Glu Lys Leu Glu Lys Gly Val Ile Cys Ser
    210                 215                 220                 
Ser Ala Gly Asn His Ala Gln Gly Val Ala Leu Ala Ala Gln Lys Leu
225                 230                 235                 240 
Gly Cys Asn Ala Val Ile Val Met Pro Val Thr Thr Pro Glu Ile Lys
                245                 250                 255     
Trp Lys Ser Val Glu Lys Leu Gly Ala Thr Val Val Leu Val Gly Asp
            260                 265                 270         
Ser Tyr Asp Glu Ala Gln Ser His Ala Lys Lys Arg Ala Lys Ser Glu
        275                 280                 285             
Gly Arg Thr Phe Ile Pro Pro Phe Asp Asn Pro Asn Val Ile Met Gly
    290                 295                 300                 
Gln Gly Thr Val Gly Met Glu Ile Ile Arg Gln Leu Arg Gly Pro Ile
305                 310                 315                 320 
His Ala Ile Phe Val Pro Val Gly Gly Gly Gly Leu Ile Ala Gly Ile
                325                 330                 335     
Ala Ala Tyr Val Lys Gln Val Arg Pro Glu Val Lys Ile Ile Gly Val
            340                 345                 350         
Glu Pro Tyr Asp Ala Asn Ala Met Ala Leu Ser Leu His His Gly Gln
        355                 360                 365             
Arg Val Met Leu Glu Gln Val Gly Gly Phe Ala Asp Gly Val Ala Val
    370                 375                 380                 
Lys Val Val Gly Glu Glu Thr Tyr Arg Leu Cys Arg Glu Leu Val Asp
385                 390                 395                 400 
Gly Ile Val Leu Val Ser Arg Asp Ala Ile Cys Ala Ser Ile Lys Asp
                405                 410                 415     
Met Phe Glu Glu Lys Arg Ser Ile Leu Glu Pro Ala Gly Ala Leu Ser
            420                 425                 430         
Leu Ala Gly Ala Glu Ala Tyr Cys Lys Tyr Tyr Gly Leu Lys Gly Glu
        435                 440                 445             
Ser Val Val Ala Ile Thr Ser Gly Ala Asn Met Asn Phe Asp Arg Leu
    450                 455                 460                 
Arg Leu Val Thr
465             


<210> 7
<211> 1236
<212> DNA
<213> Lemna minor

<220> 
<221> 5'UTR          
<222> (1)...(33)
<223> 5'UTR for glutamine synthetase 1 (GS1) isoform #1

<220> 
<221> CDS            
<222> (34)...(1104)
<223> Encodes glutamine synthetase 1 (GS1) isoform #1

<220> 
<221> 5'UTR          
<222> (1105)...(1236)
<223> 3'UTR for glutamine synthetase 1 (GS1) isoform #1

<400> 7
caatttcctc tgctcccgct tctgatccct gca atg gct ctt ctc gcc gat ctc  54
                                     Met Ala Leu Leu Ala Asp Leu
                                      1               5
 
cag aac ctg aat ctc acc gag acc acg gag aag atc atc gcc gag tac   102
Gln Asn Leu Asn Leu Thr Glu Thr Thr Glu Lys Ile Ile Ala Glu Tyr
         10                  15                  20
 
ata tgg atc ggc ggc tct ggc ttg gac atg agg agc aag gcg agg acg   150
Ile Trp Ile Gly Gly Ser Gly Leu Asp Met Arg Ser Lys Ala Arg Thr
     25                  30                  35
 
atc tcc aaa ccg gtg tct gat ccc aag gaa ctc ccc aag tgg aac tac   198
Ile Ser Lys Pro Val Ser Asp Pro Lys Glu Leu Pro Lys Trp Asn Tyr
 40                  45                  50                  55
 
gac ggc tcc agc act ggt caa gct cct gga gag gac agc gaa gtg atc   246
Asp Gly Ser Ser Thr Gly Gln Ala Pro Gly Glu Asp Ser Glu Val Ile
                 60                  65                  70
 
ctc tat ccc cag gcc atc ttc agg gat cca ttc agg aag gga aac aac   294
Leu Tyr Pro Gln Ala Ile Phe Arg Asp Pro Phe Arg Lys Gly Asn Asn
             75                  80                  85
 
att ctt gtg atg tgc gac tgc tac acg cca gcg gga gag ccg atc ccc   342
Ile Leu Val Met Cys Asp Cys Tyr Thr Pro Ala Gly Glu Pro Ile Pro
         90                  95                 100
 
acg aac aag agg tac aga gcc tct cag atc ttc agc ggt ccc gcc gtc   390
Thr Asn Lys Arg Tyr Arg Ala Ser Gln Ile Phe Ser Gly Pro Ala Val
    105                 110                 115
 
gtc gca gaa gag acc tgg tat gga cta gag cag gag tac act cta ctc   438
Val Ala Glu Glu Thr Trp Tyr Gly Leu Glu Gln Glu Tyr Thr Leu Leu
120                 125                 130                 135
 
cag aag gac gtg aag tgg cct ctg ggc tgg cct ctg ggc ggc ttc cct   486
Gln Lys Asp Val Lys Trp Pro Leu Gly Trp Pro Leu Gly Gly Phe Pro
                140                 145                 150
 
gct cca cag ggt ccg tac tac tgc ggt ata ggc gtg gac aag gcg ttc   534
Ala Pro Gln Gly Pro Tyr Tyr Cys Gly Ile Gly Val Asp Lys Ala Phe
            155                 160                 165
 
ggg aga gag atc gtc gac gcc cac tac aag gcc tgc ctg tac gca gga   582
Gly Arg Glu Ile Val Asp Ala His Tyr Lys Ala Cys Leu Tyr Ala Gly
        170                 175                 180
 
atc aac atc agc ggg atc aat ggc gaa gtc atg cct gga cag tgg gag   630
Ile Asn Ile Ser Gly Ile Asn Gly Glu Val Met Pro Gly Gln Trp Glu
    185                 190                 195
 
ttc caa gtt gga cca tcc gtc gga atc tca gcc tcc gat cag ctc tgg   678
Phe Gln Val Gly Pro Ser Val Gly Ile Ser Ala Ser Asp Gln Leu Trp
200                 205                 210                 215
 
atc gct cgc tac ctc ttg gag agg atc aca gag gtc gcc gga gtt gtt   726
Ile Ala Arg Tyr Leu Leu Glu Arg Ile Thr Glu Val Ala Gly Val Val
                220                 225                 230
 
ctc tcc ttg cac ccc aag cca atc aag ggt gac tgg aac ggc gct gga   774
Leu Ser Leu His Pro Lys Pro Ile Lys Gly Asp Trp Asn Gly Ala Gly
            235                 240                 245
 
tgc cac acc aac tac agt acc aaa tcg atg agg gag gat ggc gga tac   822
Cys His Thr Asn Tyr Ser Thr Lys Ser Met Arg Glu Asp Gly Gly Tyr
        250                 255                 260
 
gag ctg atc aag aag gcg atc gac aag ctc gga ctc agg cac aag gaa   870
Glu Leu Ile Lys Lys Ala Ile Asp Lys Leu Gly Leu Arg His Lys Glu
    265                 270                 275
 
cac atc gag gcc tac ggc gag gat aac gag gag cgt ctc act ggc cgc   918
His Ile Glu Ala Tyr Gly Glu Asp Asn Glu Glu Arg Leu Thr Gly Arg
280                 285                 290                 295
 
cac gag acc gcc gac atc cac acc ttc aaa tgg ggc gtg gcc aac cgg   966
His Glu Thr Ala Asp Ile His Thr Phe Lys Trp Gly Val Ala Asn Arg
                300                 305                 310
 
gga gct tcg atc cgc gtc gga cgg gac acg gag aag gaa gga aaa ggt   1014
Gly Ala Ser Ile Arg Val Gly Arg Asp Thr Glu Lys Glu Gly Lys Gly
            315                 320                 325
 
tac ttc gag gac agg agg ccg gct tcc aac atg gac ccg tac gtg gtg   1062
Tyr Phe Glu Asp Arg Arg Pro Ala Ser Asn Met Asp Pro Tyr Val Val
        330                 335                 340
 
acc tcc atg atc gcg gag acg acc ctt ctc tgg aag ccc tga           1104
Thr Ser Met Ile Ala Glu Thr Thr Leu Leu Trp Lys Pro  
    345                 350                 355
 
tcgcggagct tcttccatgg tcgtcctgct cgtccttctc tttcaatttc tgtcaaaaat 1164
ggctggcttt tccatccttc tggatgtgag tctgtgtcgg cggggtgagt gatcggttga 1224
cttctccccc tc                                                     1236

<210> 8
<211> 1071
<212> DNA
<213> Lemna minor

<220> 
<221> CDS            
<222> (1)...(1071)
<223> Encodes glutamine synthetase 1 (GS1) isoform #1

<400> 8
atg gct ctt ctc gcc gat ctc cag aac ctg aat ctc acc gag acc acg   48
Met Ala Leu Leu Ala Asp Leu Gln Asn Leu Asn Leu Thr Glu Thr Thr
 1               5                   10                  15
 
gag aag atc atc gcc gag tac ata tgg atc ggc ggc tct ggc ttg gac   96
Glu Lys Ile Ile Ala Glu Tyr Ile Trp Ile Gly Gly Ser Gly Leu Asp
             20                  25                  30
 
atg agg agc aag gcg agg acg atc tcc aaa ccg gtg tct gat ccc aag   144
Met Arg Ser Lys Ala Arg Thr Ile Ser Lys Pro Val Ser Asp Pro Lys
         35                  40                  45
 
gaa ctc ccc aag tgg aac tac gac ggc tcc agc act ggt caa gct cct   192
Glu Leu Pro Lys Trp Asn Tyr Asp Gly Ser Ser Thr Gly Gln Ala Pro
     50                  55                  60
 
gga gag gac agc gaa gtg atc ctc tat ccc cag gcc atc ttc agg gat   240
Gly Glu Asp Ser Glu Val Ile Leu Tyr Pro Gln Ala Ile Phe Arg Asp
 65                  70                  75                  80
 
cca ttc agg aag gga aac aac att ctt gtg atg tgc gac tgc tac acg   288
Pro Phe Arg Lys Gly Asn Asn Ile Leu Val Met Cys Asp Cys Tyr Thr
                 85                  90                  95
 
cca gcg gga gag ccg atc ccc acg aac aag agg tac aga gcc tct cag   336
Pro Ala Gly Glu Pro Ile Pro Thr Asn Lys Arg Tyr Arg Ala Ser Gln
            100                 105                 110
 
atc ttc agc ggt ccc gcc gtc gtc gca gaa gag acc tgg tat gga cta   384
Ile Phe Ser Gly Pro Ala Val Val Ala Glu Glu Thr Trp Tyr Gly Leu
        115                 120                 125
 
gag cag gag tac act cta ctc cag aag gac gtg aag tgg cct ctg ggc   432
Glu Gln Glu Tyr Thr Leu Leu Gln Lys Asp Val Lys Trp Pro Leu Gly
    130                 135                 140
 
tgg cct ctg ggc ggc ttc cct gct cca cag ggt ccg tac tac tgc ggt   480
Trp Pro Leu Gly Gly Phe Pro Ala Pro Gln Gly Pro Tyr Tyr Cys Gly
145                 150                 155                 160
 
ata ggc gtg gac aag gcg ttc ggg aga gag atc gtc gac gcc cac tac   528
Ile Gly Val Asp Lys Ala Phe Gly Arg Glu Ile Val Asp Ala His Tyr
                165                 170                 175
 
aag gcc tgc ctg tac gca gga atc aac atc agc ggg atc aat ggc gaa   576
Lys Ala Cys Leu Tyr Ala Gly Ile Asn Ile Ser Gly Ile Asn Gly Glu
            180                 185                 190
 
gtc atg cct gga cag tgg gag ttc caa gtt gga cca tcc gtc gga atc   624
Val Met Pro Gly Gln Trp Glu Phe Gln Val Gly Pro Ser Val Gly Ile
        195                 200                 205
 
tca gcc tcc gat cag ctc tgg atc gct cgc tac ctc ttg gag agg atc   672
Ser Ala Ser Asp Gln Leu Trp Ile Ala Arg Tyr Leu Leu Glu Arg Ile
    210                 215                 220
 
aca gag gtc gcc gga gtt gtt ctc tcc ttg cac ccc aag cca atc aag   720
Thr Glu Val Ala Gly Val Val Leu Ser Leu His Pro Lys Pro Ile Lys
225                 230                 235                 240
 
ggt gac tgg aac ggc gct gga tgc cac acc aac tac agt acc aaa tcg   768
Gly Asp Trp Asn Gly Ala Gly Cys His Thr Asn Tyr Ser Thr Lys Ser
                245                 250                 255
 
atg agg gag gat ggc gga tac gag ctg atc aag aag gcg atc gac aag   816
Met Arg Glu Asp Gly Gly Tyr Glu Leu Ile Lys Lys Ala Ile Asp Lys
            260                 265                 270
 
ctc gga ctc agg cac aag gaa cac atc gag gcc tac ggc gag gat aac   864
Leu Gly Leu Arg His Lys Glu His Ile Glu Ala Tyr Gly Glu Asp Asn
        275                 280                 285
 
gag gag cgt ctc act ggc cgc cac gag acc gcc gac atc cac acc ttc   912
Glu Glu Arg Leu Thr Gly Arg His Glu Thr Ala Asp Ile His Thr Phe
    290                 295                 300
 
aaa tgg ggc gtg gcc aac cgg gga gct tcg atc cgc gtc gga cgg gac   960
Lys Trp Gly Val Ala Asn Arg Gly Ala Ser Ile Arg Val Gly Arg Asp
305                 310                 315                 320
 
acg gag aag gaa gga aaa ggt tac ttc gag gac agg agg ccg gct tcc   1008
Thr Glu Lys Glu Gly Lys Gly Tyr Phe Glu Asp Arg Arg Pro Ala Ser
                325                 330                 335
 
aac atg gac ccg tac gtg gtg acc tcc atg atc gcg gag acg acc ctt   1056
Asn Met Asp Pro Tyr Val Val Thr Ser Met Ile Ala Glu Thr Thr Leu
            340                 345                 350
 
ctc tgg aag ccc tga                                               1071
Leu Trp Lys Pro  
        355
 

<210> 9
<211> 356
<212> PRT
<213> Lemna minor

<400> 9
Met Ala Leu Leu Ala Asp Leu Gln Asn Leu Asn Leu Thr Glu Thr Thr
 1               5                  10                  15      
Glu Lys Ile Ile Ala Glu Tyr Ile Trp Ile Gly Gly Ser Gly Leu Asp
            20                  25                  30          
Met Arg Ser Lys Ala Arg Thr Ile Ser Lys Pro Val Ser Asp Pro Lys
        35                  40                  45              
Glu Leu Pro Lys Trp Asn Tyr Asp Gly Ser Ser Thr Gly Gln Ala Pro
    50                  55                  60                  
Gly Glu Asp Ser Glu Val Ile Leu Tyr Pro Gln Ala Ile Phe Arg Asp
65                  70                  75                  80  
Pro Phe Arg Lys Gly Asn Asn Ile Leu Val Met Cys Asp Cys Tyr Thr
                85                  90                  95      
Pro Ala Gly Glu Pro Ile Pro Thr Asn Lys Arg Tyr Arg Ala Ser Gln
            100                 105                 110         
Ile Phe Ser Gly Pro Ala Val Val Ala Glu Glu Thr Trp Tyr Gly Leu
        115                 120                 125             
Glu Gln Glu Tyr Thr Leu Leu Gln Lys Asp Val Lys Trp Pro Leu Gly
    130                 135                 140                 
Trp Pro Leu Gly Gly Phe Pro Ala Pro Gln Gly Pro Tyr Tyr Cys Gly
145                 150                 155                 160 
Ile Gly Val Asp Lys Ala Phe Gly Arg Glu Ile Val Asp Ala His Tyr
                165                 170                 175     
Lys Ala Cys Leu Tyr Ala Gly Ile Asn Ile Ser Gly Ile Asn Gly Glu
            180                 185                 190         
Val Met Pro Gly Gln Trp Glu Phe Gln Val Gly Pro Ser Val Gly Ile
        195                 200                 205             
Ser Ala Ser Asp Gln Leu Trp Ile Ala Arg Tyr Leu Leu Glu Arg Ile
    210                 215                 220                 
Thr Glu Val Ala Gly Val Val Leu Ser Leu His Pro Lys Pro Ile Lys
225                 230                 235                 240 
Gly Asp Trp Asn Gly Ala Gly Cys His Thr Asn Tyr Ser Thr Lys Ser
                245                 250                 255     
Met Arg Glu Asp Gly Gly Tyr Glu Leu Ile Lys Lys Ala Ile Asp Lys
            260                 265                 270         
Leu Gly Leu Arg His Lys Glu His Ile Glu Ala Tyr Gly Glu Asp Asn
        275                 280                 285             
Glu Glu Arg Leu Thr Gly Arg His Glu Thr Ala Asp Ile His Thr Phe
    290                 295                 300                 
Lys Trp Gly Val Ala Asn Arg Gly Ala Ser Ile Arg Val Gly Arg Asp
305                 310                 315                 320 
Thr Glu Lys Glu Gly Lys Gly Tyr Phe Glu Asp Arg Arg Pro Ala Ser
                325                 330                 335     
Asn Met Asp Pro Tyr Val Val Thr Ser Met Ile Ala Glu Thr Thr Leu
            340                 345                 350         
Leu Trp Lys Pro
        355     


<210> 10
<211> 1233
<212> DNA
<213> Lemna minor

<220> 
<221> 5'UTR          
<222> (1)...(33)
<223> 5'UTR for glutamine synthetase 1 (GS1) isoform #2

<220> 
<221> CDS            
<222> (34)...(1104)
<223> encodes gluatmine synthetase 1 (GS1) isoform #2

<220> 
<221> 3'UTR          
<222> (1105)...(1233)
<223> 3'UTR for glutamine synthetase 1 (GS1) isoform #2

<400> 10
caatttcctc tgctcccgct tctgatctct gca atg gct ctt ctc acc gat ctc  54
                                     Met Ala Leu Leu Thr Asp Leu
                                      1               5
 
cag aac ctg aat ctc acc gag acc acg gag aag atc atc gcc gag tac   102
Gln Asn Leu Asn Leu Thr Glu Thr Thr Glu Lys Ile Ile Ala Glu Tyr
         10                  15                  20
 
ata tgg atc ggc ggc tct ggc ttg gac atg agg agc aag gcg agg acg   150
Ile Trp Ile Gly Gly Ser Gly Leu Asp Met Arg Ser Lys Ala Arg Thr
     25                  30                  35
 
atc tcc aaa ccg gtg tct gat ccc aag gaa ctc ccc aag tgg aac tac   198
Ile Ser Lys Pro Val Ser Asp Pro Lys Glu Leu Pro Lys Trp Asn Tyr
 40                  45                  50                  55
 
gac ggc tcc agc aca ggt caa gct cca gga gag gac agc gaa gtt atc   246
Asp Gly Ser Ser Thr Gly Gln Ala Pro Gly Glu Asp Ser Glu Val Ile
                 60                  65                  70
 
ctc tat ccc cag gcc atc ttc agg gat cca ttc agg aag gga aac aac   294
Leu Tyr Pro Gln Ala Ile Phe Arg Asp Pro Phe Arg Lys Gly Asn Asn
             75                  80                  85
 
att ctc gtg atg tgc gac tgc tac acg cca gcg gga gag ccg atc ccc   342
Ile Leu Val Met Cys Asp Cys Tyr Thr Pro Ala Gly Glu Pro Ile Pro
         90                  95                 100
 
acg aac aag agg tac aga gcc tct cag atc ttc agc gat ccc gcc gtc   390
Thr Asn Lys Arg Tyr Arg Ala Ser Gln Ile Phe Ser Asp Pro Ala Val
    105                 110                 115
 
gtc gca gaa gag acc tgg tat gga cta gag cag gag tac act ctc ctc   438
Val Ala Glu Glu Thr Trp Tyr Gly Leu Glu Gln Glu Tyr Thr Leu Leu
120                 125                 130                 135
 
cag aag gac gtg aaa tgg cct ctg ggc tgg cct ctg gga ggc ttc cct   486
Gln Lys Asp Val Lys Trp Pro Leu Gly Trp Pro Leu Gly Gly Phe Pro
                140                 145                 150
 
gct cca cag ggt ccg tac tac tgc ggt ata ggt gtg gac aag gcg ttc   534
Ala Pro Gln Gly Pro Tyr Tyr Cys Gly Ile Gly Val Asp Lys Ala Phe
            155                 160                 165
 
ggg agg gag atc gtc gac gcc cac tac aag gcc tgc ctg tac gct gga   582
Gly Arg Glu Ile Val Asp Ala His Tyr Lys Ala Cys Leu Tyr Ala Gly
        170                 175                 180
 
atc aac atc agc ggg atc aat ggc gaa gtc atg cct gga cag tgg gag   630
Ile Asn Ile Ser Gly Ile Asn Gly Glu Val Met Pro Gly Gln Trp Glu
    185                 190                 195
 
ttc caa gtt gga cca gcc gtc gga atc tcc gcc tcc gat cag ctc tgg   678
Phe Gln Val Gly Pro Ala Val Gly Ile Ser Ala Ser Asp Gln Leu Trp
200                 205                 210                 215
 
gtc gct cgc tac ctc ttg gag agg atc aca gag gtt gcc gga gtt gtt   726
Val Ala Arg Tyr Leu Leu Glu Arg Ile Thr Glu Val Ala Gly Val Val
                220                 225                 230
 
ctc tcc ttg cac ccc aag cca atc aag ggt gac tgg aac ggc gct gga   774
Leu Ser Leu His Pro Lys Pro Ile Lys Gly Asp Trp Asn Gly Ala Gly
            235                 240                 245
 
tgc cac acc aac tac agt acc aaa tcg atg agg gag gaa ggc gga tac   822
Cys His Thr Asn Tyr Ser Thr Lys Ser Met Arg Glu Glu Gly Gly Tyr
        250                 255                 260
 
gag ctg atc aag aag gcg atc gac aaa ctc gga ctg agg cac aag gag   870
Glu Leu Ile Lys Lys Ala Ile Asp Lys Leu Gly Leu Arg His Lys Glu
    265                 270                 275
 
cac atc ggg gcc tac ggt gaa gac aac gaa gag cgt ctc acc ggc cgc   918
His Ile Gly Ala Tyr Gly Glu Asp Asn Glu Glu Arg Leu Thr Gly Arg
280                 285                 290                 295
 
cac gag acc gcc gac atc cac acc ttc aaa tgg ggc gtg gcc aac cgg   966
His Glu Thr Ala Asp Ile His Thr Phe Lys Trp Gly Val Ala Asn Arg
                300                 305                 310
 
ggg gct tca atc cgc gcc gga agg gac acg gag aag gaa gga aaa ggt   1014
Gly Ala Ser Ile Arg Ala Gly Arg Asp Thr Glu Lys Glu Gly Lys Gly
            315                 320                 325
 
tac ttc gag gac agg agg ccg gct tcc aac atg gac ccg tac gtg gtg   1062
Tyr Phe Glu Asp Arg Arg Pro Ala Ser Asn Met Asp Pro Tyr Val Val
        330                 335                 340
 
acc tcc atg gtc gcg gag acg acc ctt ctc tgg aag ccc tga           1104
Thr Ser Met Val Ala Glu Thr Thr Leu Leu Trp Lys Pro  
    345                 350                 355
 
tcgcggagct tcttccatgg tcgtccagtt cgtcctcttt caatttctgt caaaatggct 1164
ggctttttca tccttcctgg atgtgggtct gtgtcggctg ggtgagtgat tggttgactt 1224
ctccccctc                                                         1233

<210> 11
<211> 1071
<212> DNA
<213> Lemna minor

<220> 
<221> CDS            
<222> (1)...(1071)
<223> Encodes glutamine synthetase 1 (GS1) isoform #2

<400> 11
atg gct ctt ctc acc gat ctc cag aac ctg aat ctc acc gag acc acg   48
Met Ala Leu Leu Thr Asp Leu Gln Asn Leu Asn Leu Thr Glu Thr Thr
 1               5                   10                  15
 
gag aag atc atc gcc gag tac ata tgg atc ggc ggc tct ggc ttg gac   96
Glu Lys Ile Ile Ala Glu Tyr Ile Trp Ile Gly Gly Ser Gly Leu Asp
             20                  25                  30
 
atg agg agc aag gcg agg acg atc tcc aaa ccg gtg tct gat ccc aag   144
Met Arg Ser Lys Ala Arg Thr Ile Ser Lys Pro Val Ser Asp Pro Lys
         35                  40                  45
 
gaa ctc ccc aag tgg aac tac gac ggc tcc agc aca ggt caa gct cca   192
Glu Leu Pro Lys Trp Asn Tyr Asp Gly Ser Ser Thr Gly Gln Ala Pro
     50                  55                  60
 
gga gag gac agc gaa gtt atc ctc tat ccc cag gcc atc ttc agg gat   240
Gly Glu Asp Ser Glu Val Ile Leu Tyr Pro Gln Ala Ile Phe Arg Asp
 65                  70                  75                  80
 
cca ttc agg aag gga aac aac att ctc gtg atg tgc gac tgc tac acg   288
Pro Phe Arg Lys Gly Asn Asn Ile Leu Val Met Cys Asp Cys Tyr Thr
                 85                  90                  95
 
cca gcg gga gag ccg atc ccc acg aac aag agg tac aga gcc tct cag   336
Pro Ala Gly Glu Pro Ile Pro Thr Asn Lys Arg Tyr Arg Ala Ser Gln
            100                 105                 110
 
atc ttc agc gat ccc gcc gtc gtc gca gaa gag acc tgg tat gga cta   384
Ile Phe Ser Asp Pro Ala Val Val Ala Glu Glu Thr Trp Tyr Gly Leu
        115                 120                 125
 
gag cag gag tac act ctc ctc cag aag gac gtg aaa tgg cct ctg ggc   432
Glu Gln Glu Tyr Thr Leu Leu Gln Lys Asp Val Lys Trp Pro Leu Gly
    130                 135                 140
 
tgg cct ctg gga ggc ttc cct gct cca cag ggt ccg tac tac tgc ggt   480
Trp Pro Leu Gly Gly Phe Pro Ala Pro Gln Gly Pro Tyr Tyr Cys Gly
145                 150                 155                 160
 
ata ggt gtg gac aag gcg ttc ggg agg gag atc gtc gac gcc cac tac   528
Ile Gly Val Asp Lys Ala Phe Gly Arg Glu Ile Val Asp Ala His Tyr
                165                 170                 175
 
aag gcc tgc ctg tac gct gga atc aac atc agc ggg atc aat ggc gaa   576
Lys Ala Cys Leu Tyr Ala Gly Ile Asn Ile Ser Gly Ile Asn Gly Glu
            180                 185                 190
 
gtc atg cct gga cag tgg gag ttc caa gtt gga cca gcc gtc gga atc   624
Val Met Pro Gly Gln Trp Glu Phe Gln Val Gly Pro Ala Val Gly Ile
        195                 200                 205
 
tcc gcc tcc gat cag ctc tgg gtc gct cgc tac ctc ttg gag agg atc   672
Ser Ala Ser Asp Gln Leu Trp Val Ala Arg Tyr Leu Leu Glu Arg Ile
    210                 215                 220
 
aca gag gtt gcc gga gtt gtt ctc tcc ttg cac ccc aag cca atc aag   720
Thr Glu Val Ala Gly Val Val Leu Ser Leu His Pro Lys Pro Ile Lys
225                 230                 235                 240
 
ggt gac tgg aac ggc gct gga tgc cac acc aac tac agt acc aaa tcg   768
Gly Asp Trp Asn Gly Ala Gly Cys His Thr Asn Tyr Ser Thr Lys Ser
                245                 250                 255
 
atg agg gag gaa ggc gga tac gag ctg atc aag aag gcg atc gac aaa   816
Met Arg Glu Glu Gly Gly Tyr Glu Leu Ile Lys Lys Ala Ile Asp Lys
            260                 265                 270
 
ctc gga ctg agg cac aag gag cac atc ggg gcc tac ggt gaa gac aac   864
Leu Gly Leu Arg His Lys Glu His Ile Gly Ala Tyr Gly Glu Asp Asn
        275                 280                 285
 
gaa gag cgt ctc acc ggc cgc cac gag acc gcc gac atc cac acc ttc   912
Glu Glu Arg Leu Thr Gly Arg His Glu Thr Ala Asp Ile His Thr Phe
    290                 295                 300
 
aaa tgg ggc gtg gcc aac cgg ggg gct tca atc cgc gcc gga agg gac   960
Lys Trp Gly Val Ala Asn Arg Gly Ala Ser Ile Arg Ala Gly Arg Asp
305                 310                 315                 320
 
acg gag aag gaa gga aaa ggt tac ttc gag gac agg agg ccg gct tcc   1008
Thr Glu Lys Glu Gly Lys Gly Tyr Phe Glu Asp Arg Arg Pro Ala Ser
                325                 330                 335
 
aac atg gac ccg tac gtg gtg acc tcc atg gtc gcg gag acg acc ctt   1056
Asn Met Asp Pro Tyr Val Val Thr Ser Met Val Ala Glu Thr Thr Leu
            340                 345                 350
 
ctc tgg aag ccc tga                                               1071
Leu Trp Lys Pro  
        355
 

<210> 12
<211> 356
<212> PRT
<213> Lemna minor

<400> 12
Met Ala Leu Leu Thr Asp Leu Gln Asn Leu Asn Leu Thr Glu Thr Thr
 1               5                  10                  15      
Glu Lys Ile Ile Ala Glu Tyr Ile Trp Ile Gly Gly Ser Gly Leu Asp
            20                  25                  30          
Met Arg Ser Lys Ala Arg Thr Ile Ser Lys Pro Val Ser Asp Pro Lys
        35                  40                  45              
Glu Leu Pro Lys Trp Asn Tyr Asp Gly Ser Ser Thr Gly Gln Ala Pro
    50                  55                  60                  
Gly Glu Asp Ser Glu Val Ile Leu Tyr Pro Gln Ala Ile Phe Arg Asp
65                  70                  75                  80  
Pro Phe Arg Lys Gly Asn Asn Ile Leu Val Met Cys Asp Cys Tyr Thr
                85                  90                  95      
Pro Ala Gly Glu Pro Ile Pro Thr Asn Lys Arg Tyr Arg Ala Ser Gln
            100                 105                 110         
Ile Phe Ser Asp Pro Ala Val Val Ala Glu Glu Thr Trp Tyr Gly Leu
        115                 120                 125             
Glu Gln Glu Tyr Thr Leu Leu Gln Lys Asp Val Lys Trp Pro Leu Gly
    130                 135                 140                 
Trp Pro Leu Gly Gly Phe Pro Ala Pro Gln Gly Pro Tyr Tyr Cys Gly
145                 150                 155                 160 
Ile Gly Val Asp Lys Ala Phe Gly Arg Glu Ile Val Asp Ala His Tyr
                165                 170                 175     
Lys Ala Cys Leu Tyr Ala Gly Ile Asn Ile Ser Gly Ile Asn Gly Glu
            180                 185                 190         
Val Met Pro Gly Gln Trp Glu Phe Gln Val Gly Pro Ala Val Gly Ile
        195                 200                 205             
Ser Ala Ser Asp Gln Leu Trp Val Ala Arg Tyr Leu Leu Glu Arg Ile
    210                 215                 220                 
Thr Glu Val Ala Gly Val Val Leu Ser Leu His Pro Lys Pro Ile Lys
225                 230                 235                 240 
Gly Asp Trp Asn Gly Ala Gly Cys His Thr Asn Tyr Ser Thr Lys Ser
                245                 250                 255     
Met Arg Glu Glu Gly Gly Tyr Glu Leu Ile Lys Lys Ala Ile Asp Lys
            260                 265                 270         
Leu Gly Leu Arg His Lys Glu His Ile Gly Ala Tyr Gly Glu Asp Asn
        275                 280                 285             
Glu Glu Arg Leu Thr Gly Arg His Glu Thr Ala Asp Ile His Thr Phe
    290                 295                 300                 
Lys Trp Gly Val Ala Asn Arg Gly Ala Ser Ile Arg Ala Gly Arg Asp
305                 310                 315                 320 
Thr Glu Lys Glu Gly Lys Gly Tyr Phe Glu Asp Arg Arg Pro Ala Ser
                325                 330                 335     
Asn Met Asp Pro Tyr Val Val Thr Ser Met Val Ala Glu Thr Thr Leu
            340                 345                 350         
Leu Trp Lys Pro
        355     


<210> 13
<211> 1551
<212> DNA
<213> Lemna minor

<220> 
<221> 5'UTR          
<222> (1)...(204)
<223> 5'UTR for glutamine synthetase 2 (GS2) isoform #1

<220> 
<221> CDS            
<222> (205)...(1479)
<223> Encodes glutamine synthetase 2 (GS2) isoform #1

<220> 
<221> 3'UTR          
<222> (1480)...(1551)
<223> 3'UTR for glutamine synthetase 2 (GS2) isoform #1

<400> 13
agcgagtaag ctgccgtatt ctgcatgcgt ggaccagatt gatcttagcc cctctctttt 60
atcgactcta aacaattcac acacatattc tctctccccc cctttctctc taaatcttct 120
ctcctcttca ccgacgccgc agccggagga tccacattat tctgtgtcgt ccttgctcgg 180
agtttctcga gcggaggaaa aaag atg gcg gcg cag att ccc gct cca tcg    231
                           Met Ala Ala Gln Ile Pro Ala Pro Ser
                            1               5
 
ctg cga tgc gag agg agc atc gcg atc agg cca tcg ctg gcg cgg aat   279
Leu Arg Cys Glu Arg Ser Ile Ala Ile Arg Pro Ser Leu Ala Arg Asn
 10                  15                  20                  25
 
cct ctg atg ctt gct cag aga ggc tcg ccg gcg tcc aga aaa gga gga   327
Pro Leu Met Leu Ala Gln Arg Gly Ser Pro Ala Ser Arg Lys Gly Gly
                 30                  35                  40
 
cct gtc aga tac aga ggc ttc tcc gtg cgc gcg gtg cta ggc aac cgg   375
Pro Val Arg Tyr Arg Gly Phe Ser Val Arg Ala Val Leu Gly Asn Arg
             45                  50                  55
 
aac aac gcc gtc tcg agg ctg gag gat ctt ctc aac ctc gat ctc aac   423
Asn Asn Ala Val Ser Arg Leu Glu Asp Leu Leu Asn Leu Asp Leu Asn
         60                  65                  70
 
ccc cac act gag aag atc atc gcg gag tac atc tgg att ggc gga tca   471
Pro His Thr Glu Lys Ile Ile Ala Glu Tyr Ile Trp Ile Gly Gly Ser
     75                  80                  85
 
gga atc gat gta cgc agc aaa tca agg acc atc tcc aga cca gtg gat   519
Gly Ile Asp Val Arg Ser Lys Ser Arg Thr Ile Ser Arg Pro Val Asp
 90                  95                 100                 105
 
gat cct tct gag cta ccc aag tgg aat tac gac gga tct agc act gga   567
Asp Pro Ser Glu Leu Pro Lys Trp Asn Tyr Asp Gly Ser Ser Thr Gly
                110                 115                 120
 
caa gct cca gga gaa gac agt gaa gtt atc ctc tac cct caa gca att   615
Gln Ala Pro Gly Glu Asp Ser Glu Val Ile Leu Tyr Pro Gln Ala Ile
            125                 130                 135
 
ttc aag gat cct ttc cga gga ggg aac aac atc ttg gtt atg tgc gat   663
Phe Lys Asp Pro Phe Arg Gly Gly Asn Asn Ile Leu Val Met Cys Asp
        140                 145                 150
 
gct tac aaa cca aat gga gag ccg atc ccc acg aat aaa cgg tac agg   711
Ala Tyr Lys Pro Asn Gly Glu Pro Ile Pro Thr Asn Lys Arg Tyr Arg
    155                 160                 165
 
gct gct cag atc ttc agt gac cca aag gtt gtt gcc gaa gtc cca tgg   759
Ala Ala Gln Ile Phe Ser Asp Pro Lys Val Val Ala Glu Val Pro Trp
170                 175                 180                 185
 
ttt gga att gaa caa gag tac act ttg ctc cag cca aat gtg aat tgg   807
Phe Gly Ile Glu Gln Glu Tyr Thr Leu Leu Gln Pro Asn Val Asn Trp
                190                 195                 200
 
cct ctt ggc tgg cct att gga gga tat ccc ggt cct cag ggt ccc tac   855
Pro Leu Gly Trp Pro Ile Gly Gly Tyr Pro Gly Pro Gln Gly Pro Tyr
            205                 210                 215
 
tat tgt tca gct ggt gcg gag aag tcg ttt ggg cgt gat ata tca gac   903
Tyr Cys Ser Ala Gly Ala Glu Lys Ser Phe Gly Arg Asp Ile Ser Asp
        220                 225                 230
 
gcc cac tac aaa gca tgc cta tat gct ggg att aac att agt ggt act   951
Ala His Tyr Lys Ala Cys Leu Tyr Ala Gly Ile Asn Ile Ser Gly Thr
    235                 240                 245
 
aat gca gaa gtt atg cct ggc cag tgg gaa tat caa gtg ggc cca agc   999
Asn Ala Glu Val Met Pro Gly Gln Trp Glu Tyr Gln Val Gly Pro Ser
250                 255                 260                 265
 
gtt ggt att gat gcc ggt gat cat atc tgg gtt tct aga tac att ctg   1047
Val Gly Ile Asp Ala Gly Asp His Ile Trp Val Ser Arg Tyr Ile Leu
                270                 275                 280
 
gag aga atc acg gaa caa gcc gga gtt gtc ctc tcc ctc gat cct aaa   1095
Glu Arg Ile Thr Glu Gln Ala Gly Val Val Leu Ser Leu Asp Pro Lys
            285                 290                 295
 
ccc atc gag ggt gac tgg aac ggc gct gga tgc cac acc aat tat agt   1143
Pro Ile Glu Gly Asp Trp Asn Gly Ala Gly Cys His Thr Asn Tyr Ser
        300                 305                 310
 
aca aag aca atg aga gag gag gga gga ttc gag gtg att aag aag gct   1191
Thr Lys Thr Met Arg Glu Glu Gly Gly Phe Glu Val Ile Lys Lys Ala
    315                 320                 325
 
gtg gtc aat ctc tcc ctt cgt cac aag gag cat atc agc gca tat gga   1239
Val Val Asn Leu Ser Leu Arg His Lys Glu His Ile Ser Ala Tyr Gly
330                 335                 340                 345
 
gaa gga aat gag cgg cgg ttg acg gga aaa cac gag acc gcc aac atc   1287
Glu Gly Asn Glu Arg Arg Leu Thr Gly Lys His Glu Thr Ala Asn Ile
                350                 355                 360
 
aat acc ttc tct tgg ggt gtt gcc aac cgt ggt tgc tcc gtg cgc gtg   1335
Asn Thr Phe Ser Trp Gly Val Ala Asn Arg Gly Cys Ser Val Arg Val
            365                 370                 375
 
ggt cgt gag acc gag aag gaa ggc aaa gga tac atg gaa gat cgc cgc   1383
Gly Arg Glu Thr Glu Lys Glu Gly Lys Gly Tyr Met Glu Asp Arg Arg
        380                 385                 390
 
ccc gca tcc aac atg gat cca tac gtg gtg aca tca ctt ctt gcc gag   1431
Pro Ala Ser Asn Met Asp Pro Tyr Val Val Thr Ser Leu Leu Ala Glu
    395                 400                 405
 
acg acg atc ctc tgg gag cct tct gtg gag ttg gtt gcc tcc tcc taa   1479
Thr Thr Ile Leu Trp Glu Pro Ser Val Glu Leu Val Ala Ser Ser  
410                 415                 420
 
tgatgaagaa gcatccatca tcatcgtcat catctttctt ctctcttgat ctgcccataa 1539
cgagatgagg ag                                                     1551

<210> 14
<211> 1275
<212> DNA
<213> Lemna minor

<220> 
<221> CDS            
<222> (1)...(1275)
<223> Encodes glutamine synthetase 2 (GS2) isoform #1

<400> 14
atg gcg gcg cag att ccc gct cca tcg ctg cga tgc gag agg agc atc   48
Met Ala Ala Gln Ile Pro Ala Pro Ser Leu Arg Cys Glu Arg Ser Ile
 1               5                   10                  15
 
gcg atc agg cca tcg ctg gcg cgg aat cct ctg atg ctt gct cag aga   96
Ala Ile Arg Pro Ser Leu Ala Arg Asn Pro Leu Met Leu Ala Gln Arg
             20                  25                  30
 
ggc tcg ccg gcg tcc aga aaa gga gga cct gtc aga tac aga ggc ttc   144
Gly Ser Pro Ala Ser Arg Lys Gly Gly Pro Val Arg Tyr Arg Gly Phe
         35                  40                  45
 
tcc gtg cgc gcg gtg cta ggc aac cgg aac aac gcc gtc tcg agg ctg   192
Ser Val Arg Ala Val Leu Gly Asn Arg Asn Asn Ala Val Ser Arg Leu
     50                  55                  60
 
gag gat ctt ctc aac ctc gat ctc aac ccc cac act gag aag atc atc   240
Glu Asp Leu Leu Asn Leu Asp Leu Asn Pro His Thr Glu Lys Ile Ile
 65                  70                  75                  80
 
gcg gag tac atc tgg att ggc gga tca gga atc gat gta cgc agc aaa   288
Ala Glu Tyr Ile Trp Ile Gly Gly Ser Gly Ile Asp Val Arg Ser Lys
                 85                  90                  95
 
tca agg acc atc tcc aga cca gtg gat gat cct tct gag cta ccc aag   336
Ser Arg Thr Ile Ser Arg Pro Val Asp Asp Pro Ser Glu Leu Pro Lys
            100                 105                 110
 
tgg aat tac gac gga tct agc act gga caa gct cca gga gaa gac agt   384
Trp Asn Tyr Asp Gly Ser Ser Thr Gly Gln Ala Pro Gly Glu Asp Ser
        115                 120                 125
 
gaa gtt atc ctc tac cct caa gca att ttc aag gat cct ttc cga gga   432
Glu Val Ile Leu Tyr Pro Gln Ala Ile Phe Lys Asp Pro Phe Arg Gly
    130                 135                 140
 
ggg aac aac atc ttg gtt atg tgc gat gct tac aaa cca aat gga gag   480
Gly Asn Asn Ile Leu Val Met Cys Asp Ala Tyr Lys Pro Asn Gly Glu
145                 150                 155                 160
 
ccg atc ccc acg aat aaa cgg tac agg gct gct cag atc ttc agt gac   528
Pro Ile Pro Thr Asn Lys Arg Tyr Arg Ala Ala Gln Ile Phe Ser Asp
                165                 170                 175
 
cca aag gtt gtt gcc gaa gtc cca tgg ttt gga att gaa caa gag tac   576
Pro Lys Val Val Ala Glu Val Pro Trp Phe Gly Ile Glu Gln Glu Tyr
            180                 185                 190
 
act ttg ctc cag cca aat gtg aat tgg cct ctt ggc tgg cct att gga   624
Thr Leu Leu Gln Pro Asn Val Asn Trp Pro Leu Gly Trp Pro Ile Gly
        195                 200                 205
 
gga tat ccc ggt cct cag ggt ccc tac tat tgt tca gct ggt gcg gag   672
Gly Tyr Pro Gly Pro Gln Gly Pro Tyr Tyr Cys Ser Ala Gly Ala Glu
    210                 215                 220
 
aag tcg ttt ggg cgt gat ata tca gac gcc cac tac aaa gca tgc cta   720
Lys Ser Phe Gly Arg Asp Ile Ser Asp Ala His Tyr Lys Ala Cys Leu
225                 230                 235                 240
 
tat gct ggg att aac att agt ggt act aat gca gaa gtt atg cct ggc   768
Tyr Ala Gly Ile Asn Ile Ser Gly Thr Asn Ala Glu Val Met Pro Gly
                245                 250                 255
 
cag tgg gaa tat caa gtg ggc cca agc gtt ggt att gat gcc ggt gat   816
Gln Trp Glu Tyr Gln Val Gly Pro Ser Val Gly Ile Asp Ala Gly Asp
            260                 265                 270
 
cat atc tgg gtt tct aga tac att ctg gag aga atc acg gaa caa gcc   864
His Ile Trp Val Ser Arg Tyr Ile Leu Glu Arg Ile Thr Glu Gln Ala
        275                 280                 285
 
gga gtt gtc ctc tcc ctc gat cct aaa ccc atc gag ggt gac tgg aac   912
Gly Val Val Leu Ser Leu Asp Pro Lys Pro Ile Glu Gly Asp Trp Asn
    290                 295                 300
 
ggc gct gga tgc cac acc aat tat agt aca aag aca atg aga gag gag   960
Gly Ala Gly Cys His Thr Asn Tyr Ser Thr Lys Thr Met Arg Glu Glu
305                 310                 315                 320
 
gga gga ttc gag gtg att aag aag gct gtg gtc aat ctc tcc ctt cgt   1008
Gly Gly Phe Glu Val Ile Lys Lys Ala Val Val Asn Leu Ser Leu Arg
                325                 330                 335
 
cac aag gag cat atc agc gca tat gga gaa gga aat gag cgg cgg ttg   1056
His Lys Glu His Ile Ser Ala Tyr Gly Glu Gly Asn Glu Arg Arg Leu
            340                 345                 350
 
acg gga aaa cac gag acc gcc aac atc aat acc ttc tct tgg ggt gtt   1104
Thr Gly Lys His Glu Thr Ala Asn Ile Asn Thr Phe Ser Trp Gly Val
        355                 360                 365
 
gcc aac cgt ggt tgc tcc gtg cgc gtg ggt cgt gag acc gag aag gaa   1152
Ala Asn Arg Gly Cys Ser Val Arg Val Gly Arg Glu Thr Glu Lys Glu
    370                 375                 380
 
ggc aaa gga tac atg gaa gat cgc cgc ccc gca tcc aac atg gat cca   1200
Gly Lys Gly Tyr Met Glu Asp Arg Arg Pro Ala Ser Asn Met Asp Pro
385                 390                 395                 400
 
tac gtg gtg aca tca ctt ctt gcc gag acg acg atc ctc tgg gag cct   1248
Tyr Val Val Thr Ser Leu Leu Ala Glu Thr Thr Ile Leu Trp Glu Pro
                405                 410                 415
 
tct gtg gag ttg gtt gcc tcc tcc taa                               1275
Ser Val Glu Leu Val Ala Ser Ser  
            420
 

<210> 15
<211> 424
<212> PRT
<213> Lemna minor

<400> 15
Met Ala Ala Gln Ile Pro Ala Pro Ser Leu Arg Cys Glu Arg Ser Ile
 1               5                  10                  15      
Ala Ile Arg Pro Ser Leu Ala Arg Asn Pro Leu Met Leu Ala Gln Arg
            20                  25                  30          
Gly Ser Pro Ala Ser Arg Lys Gly Gly Pro Val Arg Tyr Arg Gly Phe
        35                  40                  45              
Ser Val Arg Ala Val Leu Gly Asn Arg Asn Asn Ala Val Ser Arg Leu
    50                  55                  60                  
Glu Asp Leu Leu Asn Leu Asp Leu Asn Pro His Thr Glu Lys Ile Ile
65                  70                  75                  80  
Ala Glu Tyr Ile Trp Ile Gly Gly Ser Gly Ile Asp Val Arg Ser Lys
                85                  90                  95      
Ser Arg Thr Ile Ser Arg Pro Val Asp Asp Pro Ser Glu Leu Pro Lys
            100                 105                 110         
Trp Asn Tyr Asp Gly Ser Ser Thr Gly Gln Ala Pro Gly Glu Asp Ser
        115                 120                 125             
Glu Val Ile Leu Tyr Pro Gln Ala Ile Phe Lys Asp Pro Phe Arg Gly
    130                 135                 140                 
Gly Asn Asn Ile Leu Val Met Cys Asp Ala Tyr Lys Pro Asn Gly Glu
145                 150                 155                 160 
Pro Ile Pro Thr Asn Lys Arg Tyr Arg Ala Ala Gln Ile Phe Ser Asp
                165                 170                 175     
Pro Lys Val Val Ala Glu Val Pro Trp Phe Gly Ile Glu Gln Glu Tyr
            180                 185                 190         
Thr Leu Leu Gln Pro Asn Val Asn Trp Pro Leu Gly Trp Pro Ile Gly
        195                 200                 205             
Gly Tyr Pro Gly Pro Gln Gly Pro Tyr Tyr Cys Ser Ala Gly Ala Glu
    210                 215                 220                 
Lys Ser Phe Gly Arg Asp Ile Ser Asp Ala His Tyr Lys Ala Cys Leu
225                 230                 235                 240 
Tyr Ala Gly Ile Asn Ile Ser Gly Thr Asn Ala Glu Val Met Pro Gly
                245                 250                 255     
Gln Trp Glu Tyr Gln Val Gly Pro Ser Val Gly Ile Asp Ala Gly Asp
            260                 265                 270         
His Ile Trp Val Ser Arg Tyr Ile Leu Glu Arg Ile Thr Glu Gln Ala
        275                 280                 285             
Gly Val Val Leu Ser Leu Asp Pro Lys Pro Ile Glu Gly Asp Trp Asn
    290                 295                 300                 
Gly Ala Gly Cys His Thr Asn Tyr Ser Thr Lys Thr Met Arg Glu Glu
305                 310                 315                 320 
Gly Gly Phe Glu Val Ile Lys Lys Ala Val Val Asn Leu Ser Leu Arg
                325                 330                 335     
His Lys Glu His Ile Ser Ala Tyr Gly Glu Gly Asn Glu Arg Arg Leu
            340                 345                 350         
Thr Gly Lys His Glu Thr Ala Asn Ile Asn Thr Phe Ser Trp Gly Val
        355                 360                 365             
Ala Asn Arg Gly Cys Ser Val Arg Val Gly Arg Glu Thr Glu Lys Glu
    370                 375                 380                 
Gly Lys Gly Tyr Met Glu Asp Arg Arg Pro Ala Ser Asn Met Asp Pro
385                 390                 395                 400 
Tyr Val Val Thr Ser Leu Leu Ala Glu Thr Thr Ile Leu Trp Glu Pro
                405                 410                 415     
Ser Val Glu Leu Val Ala Ser Ser
            420                 


<210> 16
<211> 1551
<212> DNA
<213> Lemna minor

<220> 
<221> 5'UTR          
<222> (1)...(204)
<223> 5'UTR for glutamine synthetase 2 (GS2) isoform #2

<220> 
<221> CDS            
<222> (205)...(1479)
<223> Encodes glutamine synthetase 2 (GS2) isoform #2

<220> 
<221> 3'UTR          
<222> (1480)...(1551)
<223> 3'UTR for glutamine synthetase 2 (GS2) isoform #2

<400> 16
agcgagtaag ctgccgtatt ctgcatgcgt ggaccagatt gatcttagcc cctctctttt 60
atcgactcta aacaattcac acacatattc tctctccccc cctttctctc taaatcttct 120
ctcctcttca ccgacgccgc agccggagga tccacattat tctgtgtcgt ccttgctcgg 180
agtttctcga gcggaggaaa aaag atg gcg gcg cag att ccc gct cca tcg    231
                           Met Ala Ala Gln Ile Pro Ala Pro Ser
                            1               5
 
ctg cga tgc gag agg agc atc gcg atc agg cca tcg ctg gcg cgg aat   279
Leu Arg Cys Glu Arg Ser Ile Ala Ile Arg Pro Ser Leu Ala Arg Asn
 10                  15                  20                  25
 
cct ctg atg ctt gct cag aga ggc tcg ccg gcg tcc aga aaa gga gga   327
Pro Leu Met Leu Ala Gln Arg Gly Ser Pro Ala Ser Arg Lys Gly Gly
                 30                  35                  40
 
cct gtc aga tac aga ggc ttc tcc gtg cgc gcg gtg cta ggc aac cgg   375
Pro Val Arg Tyr Arg Gly Phe Ser Val Arg Ala Val Leu Gly Asn Arg
             45                  50                  55
 
aac aac gcc gtc tcg agg ctg gag gat ctt ctc aac ctc gat ctc aac   423
Asn Asn Ala Val Ser Arg Leu Glu Asp Leu Leu Asn Leu Asp Leu Asn
         60                  65                  70
 
ccc cac act gag aag atc atc gcg gag tac atc tgg att ggc gga tca   471
Pro His Thr Glu Lys Ile Ile Ala Glu Tyr Ile Trp Ile Gly Gly Ser
     75                  80                  85
 
gga atc gat gta cgc agc aaa tca agg acc atc tcc aga cca gtg gat   519
Gly Ile Asp Val Arg Ser Lys Ser Arg Thr Ile Ser Arg Pro Val Asp
 90                  95                 100                 105
 
gat cct tct gag cta ccc aag tgg aat tac gac gga tct agc act gga   567
Asp Pro Ser Glu Leu Pro Lys Trp Asn Tyr Asp Gly Ser Ser Thr Gly
                110                 115                 120
 
caa gct cca gga gaa gac agc gaa gtt atc ctc tac cct caa gca att   615
Gln Ala Pro Gly Glu Asp Ser Glu Val Ile Leu Tyr Pro Gln Ala Ile
            125                 130                 135
 
ttc aag gat cct ttc cga gga ggg aac aac atc ttg gtt atg tgt gat   663
Phe Lys Asp Pro Phe Arg Gly Gly Asn Asn Ile Leu Val Met Cys Asp
        140                 145                 150
 
gct tac aaa cca aat gga gag ccg atc ccc acg aat aaa cgg tac agg   711
Ala Tyr Lys Pro Asn Gly Glu Pro Ile Pro Thr Asn Lys Arg Tyr Arg
    155                 160                 165
 
gct gct cag atc ttt agt gac cca aag gtt gtt gcc gaa gtc cca tgg   759
Ala Ala Gln Ile Phe Ser Asp Pro Lys Val Val Ala Glu Val Pro Trp
170                 175                 180                 185
 
ttt gga att gaa caa gaa tac act ttg ctc cag ccg aat gtg aat tgg   807
Phe Gly Ile Glu Gln Glu Tyr Thr Leu Leu Gln Pro Asn Val Asn Trp
                190                 195                 200
 
cct ctt ggc tgg cct att gga gga tat cct gga cct cag ggt ccc tac   855
Pro Leu Gly Trp Pro Ile Gly Gly Tyr Pro Gly Pro Gln Gly Pro Tyr
            205                 210                 215
 
tat tgt tca gct ggt gcg gat aag tcg ttt ggg cgt gat ata tca gac   903
Tyr Cys Ser Ala Gly Ala Asp Lys Ser Phe Gly Arg Asp Ile Ser Asp
        220                 225                 230
 
gcc cac tac aaa gcg tgc cta tat gct ggg att aac att agt ggt act   951
Ala His Tyr Lys Ala Cys Leu Tyr Ala Gly Ile Asn Ile Ser Gly Thr
    235                 240                 245
 
aat gca gaa gtt atg cct ggc cag tgg gaa tat caa gtg ggc cca agc   999
Asn Ala Glu Val Met Pro Gly Gln Trp Glu Tyr Gln Val Gly Pro Ser
250                 255                 260                 265
 
gct gga att gat gct gga gat cat atc tgg gtc tct aga tac att ctg   1047
Ala Gly Ile Asp Ala Gly Asp His Ile Trp Val Ser Arg Tyr Ile Leu
                270                 275                 280
 
gag aga atc acg gag caa gcc gga gtt gtg ctc tcc ctc gat cct aaa   1095
Glu Arg Ile Thr Glu Gln Ala Gly Val Val Leu Ser Leu Asp Pro Lys
            285                 290                 295
 
ccc atc gag ggt gac tgg aac ggc gct gga tgc cac acc aat tac agt   1143
Pro Ile Glu Gly Asp Trp Asn Gly Ala Gly Cys His Thr Asn Tyr Ser
        300                 305                 310
 
aca aag aca atg aga gag gat gga gga ttc gag gag att aag aag gct   1191
Thr Lys Thr Met Arg Glu Asp Gly Gly Phe Glu Glu Ile Lys Lys Ala
    315                 320                 325
 
gtg gtc aat ctc tct ctt cgt cac aag gag cat att agc gcg tat gga   1239
Val Val Asn Leu Ser Leu Arg His Lys Glu His Ile Ser Ala Tyr Gly
330                 335                 340                 345
 
gaa gga aac gag cgg cgg ttg acg gga aaa cac gag acc gcc aac atc   1287
Glu Gly Asn Glu Arg Arg Leu Thr Gly Lys His Glu Thr Ala Asn Ile
                350                 355                 360
 
aat acc ttc tct tgg ggt gtt gcc aac cgt ggt tgc tct gtg cgc gtg   1335
Asn Thr Phe Ser Trp Gly Val Ala Asn Arg Gly Cys Ser Val Arg Val
            365                 370                 375
 
ggt cgt gag acc gag aag gaa ggc aaa gga tac atg gaa gat cgc cgc   1383
Gly Arg Glu Thr Glu Lys Glu Gly Lys Gly Tyr Met Glu Asp Arg Arg
        380                 385                 390
 
ccc gca tcc aac atg gat cca tac gtg gtg aca tca ctt ctt gcc gag   1431
Pro Ala Ser Asn Met Asp Pro Tyr Val Val Thr Ser Leu Leu Ala Glu
    395                 400                 405
 
acg acg atc ctc tgg gag cct tct gtg gag ttg gtt gcc tcc tcc taa   1479
Thr Thr Ile Leu Trp Glu Pro Ser Val Glu Leu Val Ala Ser Ser  
410                 415                 420
 
tgatgaagaa gcatccatca tcgtcgtcat catctttctt ctctcttgat ctgcccataa 1539
cgagatgagg ag                                                     1551

<210> 17
<211> 1275
<212> DNA
<213> Lemna minor

<220> 
<221> CDS            
<222> (1)...(1275)
<223> Encodes glutamine synthetase 2 (GS2) isoform #2

<400> 17
atg gcg gcg cag att ccc gct cca tcg ctg cga tgc gag agg agc atc   48
Met Ala Ala Gln Ile Pro Ala Pro Ser Leu Arg Cys Glu Arg Ser Ile
 1               5                   10                  15
 
gcg atc agg cca tcg ctg gcg cgg aat cct ctg atg ctt gct cag aga   96
Ala Ile Arg Pro Ser Leu Ala Arg Asn Pro Leu Met Leu Ala Gln Arg
             20                  25                  30
 
ggc tcg ccg gcg tcc aga aaa gga gga cct gtc aga tac aga ggc ttc   144
Gly Ser Pro Ala Ser Arg Lys Gly Gly Pro Val Arg Tyr Arg Gly Phe
         35                  40                  45
 
tcc gtg cgc gcg gtg cta ggc aac cgg aac aac gcc gtc tcg agg ctg   192
Ser Val Arg Ala Val Leu Gly Asn Arg Asn Asn Ala Val Ser Arg Leu
     50                  55                  60
 
gag gat ctt ctc aac ctc gat ctc aac ccc cac act gag aag atc atc   240
Glu Asp Leu Leu Asn Leu Asp Leu Asn Pro His Thr Glu Lys Ile Ile
 65                  70                  75                  80
 
gcg gag tac atc tgg att ggc gga tca gga atc gat gta cgc agc aaa   288
Ala Glu Tyr Ile Trp Ile Gly Gly Ser Gly Ile Asp Val Arg Ser Lys
                 85                  90                  95
 
tca agg acc atc tcc aga cca gtg gat gat cct tct gag cta ccc aag   336
Ser Arg Thr Ile Ser Arg Pro Val Asp Asp Pro Ser Glu Leu Pro Lys
            100                 105                 110
 
tgg aat tac gac gga tct agc act gga caa gct cca gga gaa gac agc   384
Trp Asn Tyr Asp Gly Ser Ser Thr Gly Gln Ala Pro Gly Glu Asp Ser
        115                 120                 125
 
gaa gtt atc ctc tac cct caa gca att ttc aag gat cct ttc cga gga   432
Glu Val Ile Leu Tyr Pro Gln Ala Ile Phe Lys Asp Pro Phe Arg Gly
    130                 135                 140
 
ggg aac aac atc ttg gtt atg tgt gat gct tac aaa cca aat gga gag   480
Gly Asn Asn Ile Leu Val Met Cys Asp Ala Tyr Lys Pro Asn Gly Glu
145                 150                 155                 160
 
ccg atc ccc acg aat aaa cgg tac agg gct gct cag atc ttt agt gac   528
Pro Ile Pro Thr Asn Lys Arg Tyr Arg Ala Ala Gln Ile Phe Ser Asp
                165                 170                 175
 
cca aag gtt gtt gcc gaa gtc cca tgg ttt gga att gaa caa gaa tac   576
Pro Lys Val Val Ala Glu Val Pro Trp Phe Gly Ile Glu Gln Glu Tyr
            180                 185                 190
 
act ttg ctc cag ccg aat gtg aat tgg cct ctt ggc tgg cct att gga   624
Thr Leu Leu Gln Pro Asn Val Asn Trp Pro Leu Gly Trp Pro Ile Gly
        195                 200                 205
 
gga tat cct gga cct cag ggt ccc tac tat tgt tca gct ggt gcg gat   672
Gly Tyr Pro Gly Pro Gln Gly Pro Tyr Tyr Cys Ser Ala Gly Ala Asp
    210                 215                 220
 
aag tcg ttt ggg cgt gat ata tca gac gcc cac tac aaa gcg tgc cta   720
Lys Ser Phe Gly Arg Asp Ile Ser Asp Ala His Tyr Lys Ala Cys Leu
225                 230                 235                 240
 
tat gct ggg att aac att agt ggt act aat gca gaa gtt atg cct ggc   768
Tyr Ala Gly Ile Asn Ile Ser Gly Thr Asn Ala Glu Val Met Pro Gly
                245                 250                 255
 
cag tgg gaa tat caa gtg ggc cca agc gct gga att gat gct gga gat   816
Gln Trp Glu Tyr Gln Val Gly Pro Ser Ala Gly Ile Asp Ala Gly Asp
            260                 265                 270
 
cat atc tgg gtc tct aga tac att ctg gag aga atc acg gag caa gcc   864
His Ile Trp Val Ser Arg Tyr Ile Leu Glu Arg Ile Thr Glu Gln Ala
        275                 280                 285
 
gga gtt gtg ctc tcc ctc gat cct aaa ccc atc gag ggt gac tgg aac   912
Gly Val Val Leu Ser Leu Asp Pro Lys Pro Ile Glu Gly Asp Trp Asn
    290                 295                 300
 
ggc gct gga tgc cac acc aat tac agt aca aag aca atg aga gag gat   960
Gly Ala Gly Cys His Thr Asn Tyr Ser Thr Lys Thr Met Arg Glu Asp
305                 310                 315                 320
 
gga gga ttc gag gag att aag aag gct gtg gtc aat ctc tct ctt cgt   1008
Gly Gly Phe Glu Glu Ile Lys Lys Ala Val Val Asn Leu Ser Leu Arg
                325                 330                 335
 
cac aag gag cat att agc gcg tat gga gaa gga aac gag cgg cgg ttg   1056
His Lys Glu His Ile Ser Ala Tyr Gly Glu Gly Asn Glu Arg Arg Leu
            340                 345                 350
 
acg gga aaa cac gag acc gcc aac atc aat acc ttc tct tgg ggt gtt   1104
Thr Gly Lys His Glu Thr Ala Asn Ile Asn Thr Phe Ser Trp Gly Val
        355                 360                 365
 
gcc aac cgt ggt tgc tct gtg cgc gtg ggt cgt gag acc gag aag gaa   1152
Ala Asn Arg Gly Cys Ser Val Arg Val Gly Arg Glu Thr Glu Lys Glu
    370                 375                 380
 
ggc aaa gga tac atg gaa gat cgc cgc ccc gca tcc aac atg gat cca   1200
Gly Lys Gly Tyr Met Glu Asp Arg Arg Pro Ala Ser Asn Met Asp Pro
385                 390                 395                 400
 
tac gtg gtg aca tca ctt ctt gcc gag acg acg atc ctc tgg gag cct   1248
Tyr Val Val Thr Ser Leu Leu Ala Glu Thr Thr Ile Leu Trp Glu Pro
                405                 410                 415
 
tct gtg gag ttg gtt gcc tcc tcc taa                               1275
Ser Val Glu Leu Val Ala Ser Ser  
            420
 

<210> 18
<211> 424
<212> PRT
<213> Lemna minor

<400> 18
Met Ala Ala Gln Ile Pro Ala Pro Ser Leu Arg Cys Glu Arg Ser Ile
 1               5                  10                  15      
Ala Ile Arg Pro Ser Leu Ala Arg Asn Pro Leu Met Leu Ala Gln Arg
            20                  25                  30          
Gly Ser Pro Ala Ser Arg Lys Gly Gly Pro Val Arg Tyr Arg Gly Phe
        35                  40                  45              
Ser Val Arg Ala Val Leu Gly Asn Arg Asn Asn Ala Val Ser Arg Leu
    50                  55                  60                  
Glu Asp Leu Leu Asn Leu Asp Leu Asn Pro His Thr Glu Lys Ile Ile
65                  70                  75                  80  
Ala Glu Tyr Ile Trp Ile Gly Gly Ser Gly Ile Asp Val Arg Ser Lys
                85                  90                  95      
Ser Arg Thr Ile Ser Arg Pro Val Asp Asp Pro Ser Glu Leu Pro Lys
            100                 105                 110         
Trp Asn Tyr Asp Gly Ser Ser Thr Gly Gln Ala Pro Gly Glu Asp Ser
        115                 120                 125             
Glu Val Ile Leu Tyr Pro Gln Ala Ile Phe Lys Asp Pro Phe Arg Gly
    130                 135                 140                 
Gly Asn Asn Ile Leu Val Met Cys Asp Ala Tyr Lys Pro Asn Gly Glu
145                 150                 155                 160 
Pro Ile Pro Thr Asn Lys Arg Tyr Arg Ala Ala Gln Ile Phe Ser Asp
                165                 170                 175     
Pro Lys Val Val Ala Glu Val Pro Trp Phe Gly Ile Glu Gln Glu Tyr
            180                 185                 190         
Thr Leu Leu Gln Pro Asn Val Asn Trp Pro Leu Gly Trp Pro Ile Gly
        195                 200                 205             
Gly Tyr Pro Gly Pro Gln Gly Pro Tyr Tyr Cys Ser Ala Gly Ala Asp
    210                 215                 220                 
Lys Ser Phe Gly Arg Asp Ile Ser Asp Ala His Tyr Lys Ala Cys Leu
225                 230                 235                 240 
Tyr Ala Gly Ile Asn Ile Ser Gly Thr Asn Ala Glu Val Met Pro Gly
                245                 250                 255     
Gln Trp Glu Tyr Gln Val Gly Pro Ser Ala Gly Ile Asp Ala Gly Asp
            260                 265                 270         
His Ile Trp Val Ser Arg Tyr Ile Leu Glu Arg Ile Thr Glu Gln Ala
        275                 280                 285             
Gly Val Val Leu Ser Leu Asp Pro Lys Pro Ile Glu Gly Asp Trp Asn
    290                 295                 300                 
Gly Ala Gly Cys His Thr Asn Tyr Ser Thr Lys Thr Met Arg Glu Asp
305                 310                 315                 320 
Gly Gly Phe Glu Glu Ile Lys Lys Ala Val Val Asn Leu Ser Leu Arg
                325                 330                 335     
His Lys Glu His Ile Ser Ala Tyr Gly Glu Gly Asn Glu Arg Arg Leu
            340                 345                 350         
Thr Gly Lys His Glu Thr Ala Asn Ile Asn Thr Phe Ser Trp Gly Val
        355                 360                 365             
Ala Asn Arg Gly Cys Ser Val Arg Val Gly Arg Glu Thr Glu Lys Glu
    370                 375                 380                 
Gly Lys Gly Tyr Met Glu Asp Arg Arg Pro Ala Ser Asn Met Asp Pro
385                 390                 395                 400 
Tyr Val Val Thr Ser Leu Leu Ala Glu Thr Thr Ile Leu Trp Glu Pro
                405                 410                 415     
Ser Val Glu Leu Val Ala Ser Ser
            420                 


<210> 19
<211> 1266
<212> DNA
<213> Lemna minor

<220> 
<221> 5'UTR          
<222> (1)...(53)
<223> 5'UTR for biotin synthase (BS) isoform #1

<220> 
<221> CDS            
<222> (54)...(1187)
<223> Encodes biotin synthase (BS) isoform #1

<220> 
<221> 3'UTR          
<222> (1188)...(1266)
<223> 3'UTR for biotin synthase (BS) isoform #1

<400> 19
ctgccattag cgtggggaga tacgcggaga gatcggaggc agaggattag aag atg    56
                                                           Met
                                                            1
 
ctg ttg atc cgg tct ctc cga gcg cga gtc cat cgc tcg tcc tcg agc   104
Leu Leu Ile Arg Ser Leu Arg Ala Arg Val His Arg Ser Ser Ser Ser
             5                   10                  15
 
ttc gcc ttc tcc acg gct gcc gca tcg gcg gcg act gtg cag gcg gaa   152
Phe Ala Phe Ser Thr Ala Ala Ala Ser Ala Ala Thr Val Gln Ala Glu
         20                  25                  30
 
cga acg ata agg gat ggg ccg agg act gat tgg agc aag gac gag gtc   200
Arg Thr Ile Arg Asp Gly Pro Arg Thr Asp Trp Ser Lys Asp Glu Val
     35                  40                  45
 
aaa gcg gtt tac gat tct ccc gtc ctc gat ctc ctt ttc cat ggc gcc   248
Lys Ala Val Tyr Asp Ser Pro Val Leu Asp Leu Leu Phe His Gly Ala
 50                  55                  60                  65
 
caa gtc cac agg cac gtg cac aag ttc agg gaa gtg caa cag tgt act   296
Gln Val His Arg His Val His Lys Phe Arg Glu Val Gln Gln Cys Thr
                 70                  75                  80
 
ctt ctc tcc atc aag aca ggt ggg tgc agc gaa gat tat tca tat tgc   344
Leu Leu Ser Ile Lys Thr Gly Gly Cys Ser Glu Asp Tyr Ser Tyr Cys
             85                  90                  95
 
ccg caa tcg tct cgc tat gat acg ggg ttg aaa gct caa agg ctc atg   392
Pro Gln Ser Ser Arg Tyr Asp Thr Gly Leu Lys Ala Gln Arg Leu Met
        100                 105                 110
 
acc aag gat gat gtt ctg gaa gca gca aaa aag gca aaa gat gct ggc   440
Thr Lys Asp Asp Val Leu Glu Ala Ala Lys Lys Ala Lys Asp Ala Gly
    115                 120                 125
 
agc aca cgt ttc tgc atg ggg gct gca tgg cgg gat aca att ggc cgg   488
Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Arg Asp Thr Ile Gly Arg
130                 135                 140                 145
 
aaa acc aac ttc aac cag att ctc aat tac gtc aaa gaa att agg gag   536
Lys Thr Asn Phe Asn Gln Ile Leu Asn Tyr Val Lys Glu Ile Arg Glu
                150                 155                 160
 
atg ggc atg gag gtg tgt tgc act cta ggc atg cta gag aag cag caa   584
Met Gly Met Glu Val Cys Cys Thr Leu Gly Met Leu Glu Lys Gln Gln
            165                 170                 175
 
gct gag gag ctt aag aaa gca ggg ctt acg gcg tat aat cac aat ctt   632
Ala Glu Glu Leu Lys Lys Ala Gly Leu Thr Ala Tyr Asn His Asn Leu
        180                 185                 190
 
gac act tca aga gag tat tat ccc aac att ata acc aca aga tca ttt   680
Asp Thr Ser Arg Glu Tyr Tyr Pro Asn Ile Ile Thr Thr Arg Ser Phe
    195                 200                 205
 
gat gaa cgg ctg gaa acc ctc caa cat gtt cgt gag gca gga ata agt   728
Asp Glu Arg Leu Glu Thr Leu Gln His Val Arg Glu Ala Gly Ile Ser
210                 215                 220                 225
 
gtc tgc tcc ggt gga ata att ggg ctg ggt gaa gca gaa gaa gac cgg   776
Val Cys Ser Gly Gly Ile Ile Gly Leu Gly Glu Ala Glu Glu Asp Arg
                230                 235                 240
 
gtt gga ctc ctg cac act cta gcc acc ctc cct act cat cca gag agc   824
Val Gly Leu Leu His Thr Leu Ala Thr Leu Pro Thr His Pro Glu Ser
            245                 250                 255
 
gta ccc att aat gca ctc gta cca gtt aag ggc act ccc ctc caa gat   872
Val Pro Ile Asn Ala Leu Val Pro Val Lys Gly Thr Pro Leu Gln Asp
        260                 265                 270
 
caa aag cct gtg gag atc tgg gag atg atc agg atg acc gca acg gcg   920
Gln Lys Pro Val Glu Ile Trp Glu Met Ile Arg Met Thr Ala Thr Ala
    275                 280                 285
 
cgc atc gtg atg cca caa gca atg gtg cgg ctc tca gca ggc cga gtt   968
Arg Ile Val Met Pro Gln Ala Met Val Arg Leu Ser Ala Gly Arg Val
290                 295                 300                 305
 
cgc ttc tcc atg ccc gag cag gcc ctt tgc ttc ctc gca ggc gcc aac   1016
Arg Phe Ser Met Pro Glu Gln Ala Leu Cys Phe Leu Ala Gly Ala Asn
                310                 315                 320
 
tcc atc ttc acc gga gaa aag ctt ctc acc act gcc aac aac gac ttt   1064
Ser Ile Phe Thr Gly Glu Lys Leu Leu Thr Thr Ala Asn Asn Asp Phe
            325                 330                 335
 
gac gca gat cag ctc atg ttc aaa gtt ctc ggt ctc att ccc aag gca   1112
Asp Ala Asp Gln Leu Met Phe Lys Val Leu Gly Leu Ile Pro Lys Ala
        340                 345                 350
 
cct agc ttt tct gag gag ttt cag gag acg gcg gca gag agc cct gag   1160
Pro Ser Phe Ser Glu Glu Phe Gln Glu Thr Ala Ala Glu Ser Pro Glu
    355                 360                 365
 
ctg gcg gcg gtt tca agt tcc ggt tga attctccgag ctagcattaa         1207
Leu Ala Ala Val Ser Ser Ser Gly  
370                 375
 
gtatttgaac ctcagaacaa aggcggtaat tagtacttga ggtgagctta tatgaggga  1266

<210> 20
<211> 1134
<212> DNA
<213> Lemna minor

<220> 
<221> CDS            
<222> (1)...(1134)
<223> Encodes biotin synthase (BS) isoform #1

<400> 20
atg ctg ttg atc cgg tct ctc cga gcg cga gtc cat cgc tcg tcc tcg   48
Met Leu Leu Ile Arg Ser Leu Arg Ala Arg Val His Arg Ser Ser Ser
 1               5                   10                  15
 
agc ttc gcc ttc tcc acg gct gcc gca tcg gcg gcg act gtg cag gcg   96
Ser Phe Ala Phe Ser Thr Ala Ala Ala Ser Ala Ala Thr Val Gln Ala
             20                  25                  30
 
gaa cga acg ata agg gat ggg ccg agg act gat tgg agc aag gac gag   144
Glu Arg Thr Ile Arg Asp Gly Pro Arg Thr Asp Trp Ser Lys Asp Glu
         35                  40                  45
 
gtc aaa gcg gtt tac gat tct ccc gtc ctc gat ctc ctt ttc cat ggc   192
Val Lys Ala Val Tyr Asp Ser Pro Val Leu Asp Leu Leu Phe His Gly
     50                  55                  60
 
gcc caa gtc cac agg cac gtg cac aag ttc agg gaa gtg caa cag tgt   240
Ala Gln Val His Arg His Val His Lys Phe Arg Glu Val Gln Gln Cys
 65                  70                  75                  80
 
act ctt ctc tcc atc aag aca ggt ggg tgc agc gaa gat tat tca tat   288
Thr Leu Leu Ser Ile Lys Thr Gly Gly Cys Ser Glu Asp Tyr Ser Tyr
                 85                  90                  95
 
tgc ccg caa tcg tct cgc tat gat acg ggg ttg aaa gct caa agg ctc   336
Cys Pro Gln Ser Ser Arg Tyr Asp Thr Gly Leu Lys Ala Gln Arg Leu
            100                 105                 110
 
atg acc aag gat gat gtt ctg gaa gca gca aaa aag gca aaa gat gct   384
Met Thr Lys Asp Asp Val Leu Glu Ala Ala Lys Lys Ala Lys Asp Ala
        115                 120                 125
 
ggc agc aca cgt ttc tgc atg ggg gct gca tgg cgg gat aca att ggc   432
Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Arg Asp Thr Ile Gly
    130                 135                 140
 
cgg aaa acc aac ttc aac cag att ctc aat tac gtc aaa gaa att agg   480
Arg Lys Thr Asn Phe Asn Gln Ile Leu Asn Tyr Val Lys Glu Ile Arg
145                 150                 155                 160
 
gag atg ggc atg gag gtg tgt tgc act cta ggc atg cta gag aag cag   528
Glu Met Gly Met Glu Val Cys Cys Thr Leu Gly Met Leu Glu Lys Gln
                165                 170                 175
 
caa gct gag gag ctt aag aaa gca ggg ctt acg gcg tat aat cac aat   576
Gln Ala Glu Glu Leu Lys Lys Ala Gly Leu Thr Ala Tyr Asn His Asn
            180                 185                 190
 
ctt gac act tca aga gag tat tat ccc aac att ata acc aca aga tca   624
Leu Asp Thr Ser Arg Glu Tyr Tyr Pro Asn Ile Ile Thr Thr Arg Ser
        195                 200                 205
 
ttt gat gaa cgg ctg gaa acc ctc caa cat gtt cgt gag gca gga ata   672
Phe Asp Glu Arg Leu Glu Thr Leu Gln His Val Arg Glu Ala Gly Ile
    210                 215                 220
 
agt gtc tgc tcc ggt gga ata att ggg ctg ggt gaa gca gaa gaa gac   720
Ser Val Cys Ser Gly Gly Ile Ile Gly Leu Gly Glu Ala Glu Glu Asp
225                 230                 235                 240
 
cgg gtt gga ctc ctg cac act cta gcc acc ctc cct act cat cca gag   768
Arg Val Gly Leu Leu His Thr Leu Ala Thr Leu Pro Thr His Pro Glu
                245                 250                 255
 
agc gta ccc att aat gca ctc gta cca gtt aag ggc act ccc ctc caa   816
Ser Val Pro Ile Asn Ala Leu Val Pro Val Lys Gly Thr Pro Leu Gln
            260                 265                 270
 
gat caa aag cct gtg gag atc tgg gag atg atc agg atg acc gca acg   864
Asp Gln Lys Pro Val Glu Ile Trp Glu Met Ile Arg Met Thr Ala Thr
        275                 280                 285
 
gcg cgc atc gtg atg cca caa gca atg gtg cgg ctc tca gca ggc cga   912
Ala Arg Ile Val Met Pro Gln Ala Met Val Arg Leu Ser Ala Gly Arg
    290                 295                 300
 
gtt cgc ttc tcc atg ccc gag cag gcc ctt tgc ttc ctc gca ggc gcc   960
Val Arg Phe Ser Met Pro Glu Gln Ala Leu Cys Phe Leu Ala Gly Ala
305                 310                 315                 320
 
aac tcc atc ttc acc gga gaa aag ctt ctc acc act gcc aac aac gac   1008
Asn Ser Ile Phe Thr Gly Glu Lys Leu Leu Thr Thr Ala Asn Asn Asp
                325                 330                 335
 
ttt gac gca gat cag ctc atg ttc aaa gtt ctc ggt ctc att ccc aag   1056
Phe Asp Ala Asp Gln Leu Met Phe Lys Val Leu Gly Leu Ile Pro Lys
            340                 345                 350
 
gca cct agc ttt tct gag gag ttt cag gag acg gcg gca gag agc cct   1104
Ala Pro Ser Phe Ser Glu Glu Phe Gln Glu Thr Ala Ala Glu Ser Pro
        355                 360                 365
 
gag ctg gcg gcg gtt tca agt tcc ggt tga                           1134
Glu Leu Ala Ala Val Ser Ser Ser Gly  
    370                 375
 

<210> 21
<211> 377
<212> PRT
<213> Lemna minor

<400> 21
Met Leu Leu Ile Arg Ser Leu Arg Ala Arg Val His Arg Ser Ser Ser
 1               5                  10                  15      
Ser Phe Ala Phe Ser Thr Ala Ala Ala Ser Ala Ala Thr Val Gln Ala
            20                  25                  30          
Glu Arg Thr Ile Arg Asp Gly Pro Arg Thr Asp Trp Ser Lys Asp Glu
        35                  40                  45              
Val Lys Ala Val Tyr Asp Ser Pro Val Leu Asp Leu Leu Phe His Gly
    50                  55                  60                  
Ala Gln Val His Arg His Val His Lys Phe Arg Glu Val Gln Gln Cys
65                  70                  75                  80  
Thr Leu Leu Ser Ile Lys Thr Gly Gly Cys Ser Glu Asp Tyr Ser Tyr
                85                  90                  95      
Cys Pro Gln Ser Ser Arg Tyr Asp Thr Gly Leu Lys Ala Gln Arg Leu
            100                 105                 110         
Met Thr Lys Asp Asp Val Leu Glu Ala Ala Lys Lys Ala Lys Asp Ala
        115                 120                 125             
Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Arg Asp Thr Ile Gly
    130                 135                 140                 
Arg Lys Thr Asn Phe Asn Gln Ile Leu Asn Tyr Val Lys Glu Ile Arg
145                 150                 155                 160 
Glu Met Gly Met Glu Val Cys Cys Thr Leu Gly Met Leu Glu Lys Gln
                165                 170                 175     
Gln Ala Glu Glu Leu Lys Lys Ala Gly Leu Thr Ala Tyr Asn His Asn
            180                 185                 190         
Leu Asp Thr Ser Arg Glu Tyr Tyr Pro Asn Ile Ile Thr Thr Arg Ser
        195                 200                 205             
Phe Asp Glu Arg Leu Glu Thr Leu Gln His Val Arg Glu Ala Gly Ile
    210                 215                 220                 
Ser Val Cys Ser Gly Gly Ile Ile Gly Leu Gly Glu Ala Glu Glu Asp
225                 230                 235                 240 
Arg Val Gly Leu Leu His Thr Leu Ala Thr Leu Pro Thr His Pro Glu
                245                 250                 255     
Ser Val Pro Ile Asn Ala Leu Val Pro Val Lys Gly Thr Pro Leu Gln
            260                 265                 270         
Asp Gln Lys Pro Val Glu Ile Trp Glu Met Ile Arg Met Thr Ala Thr
        275                 280                 285             
Ala Arg Ile Val Met Pro Gln Ala Met Val Arg Leu Ser Ala Gly Arg
    290                 295                 300                 
Val Arg Phe Ser Met Pro Glu Gln Ala Leu Cys Phe Leu Ala Gly Ala
305                 310                 315                 320 
Asn Ser Ile Phe Thr Gly Glu Lys Leu Leu Thr Thr Ala Asn Asn Asp
                325                 330                 335     
Phe Asp Ala Asp Gln Leu Met Phe Lys Val Leu Gly Leu Ile Pro Lys
            340                 345                 350         
Ala Pro Ser Phe Ser Glu Glu Phe Gln Glu Thr Ala Ala Glu Ser Pro
        355                 360                 365             
Glu Leu Ala Ala Val Ser Ser Ser Gly
    370                 375         


<210> 22
<211> 1266
<212> DNA
<213> Lemna minor

<220> 
<221> 5'UTR          
<222> (1)...(53)
<223> 5'UTR for biotin synthase (BS) isoform #2

<220> 
<221> CDS            
<222> (54)...(1187)
<223> Encodes biotine synthase (BS) isoform #2

<220> 
<221> 3'UTR          
<222> (1188)...(1266)
<223> 3'UTR for biotin synthase (BS) isoform #2

<400> 22
ctgccattag cgtggggaga tacgcggaga gatcggaggc agaggattag aag atg    56
                                                           Met
                                                            1
 
ctg ttg atc cgg tct ctc cga gcg cga gtc cat cgc tcg tcc tcg agc   104
Leu Leu Ile Arg Ser Leu Arg Ala Arg Val His Arg Ser Ser Ser Ser
             5                   10                  15
 
ttc gcc ttc tcc acg gct gcc gca tcg gcg gcg act gtg cag gcg gaa   152
Phe Ala Phe Ser Thr Ala Ala Ala Ser Ala Ala Thr Val Gln Ala Glu
         20                  25                  30
 
cga acg ata agg gat ggg ccg agg act gat tgg agc aag gac gag gtc   200
Arg Thr Ile Arg Asp Gly Pro Arg Thr Asp Trp Ser Lys Asp Glu Val
     35                  40                  45
 
aaa gcg gtt tac gat tct ccc gtc ctc gat ctc ctt ttc cat ggc gcc   248
Lys Ala Val Tyr Asp Ser Pro Val Leu Asp Leu Leu Phe His Gly Ala
 50                  55                  60                  65
 
caa gtc cac agg cac gtg cac aag ttc agg gaa gtg caa cag tgt act   296
Gln Val His Arg His Val His Lys Phe Arg Glu Val Gln Gln Cys Thr
                 70                  75                  80
 
ctt ctc tcc atc aag aca ggt ggg tgc agc gaa gat tgt tca tat tgc   344
Leu Leu Ser Ile Lys Thr Gly Gly Cys Ser Glu Asp Cys Ser Tyr Cys
             85                  90                  95
 
ccg caa tcg tct cgc tat gat acg ggg ttg aaa gct caa agg ctc atg   392
Pro Gln Ser Ser Arg Tyr Asp Thr Gly Leu Lys Ala Gln Arg Leu Met
        100                 105                 110
 
acc aag gat gat gtt ctg gaa gca gca aaa aag gca aaa gat gct ggc   440
Thr Lys Asp Asp Val Leu Glu Ala Ala Lys Lys Ala Lys Asp Ala Gly
    115                 120                 125
 
agc aca cgt ttc tgc atg ggg gct gca tgg cgg gat aca att ggc cgg   488
Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Arg Asp Thr Ile Gly Arg
130                 135                 140                 145
 
aaa acc aac ttc aac cag att ctc aat tac gtc aaa gaa att agg gag   536
Lys Thr Asn Phe Asn Gln Ile Leu Asn Tyr Val Lys Glu Ile Arg Glu
                150                 155                 160
 
atg ggc atg gag gtg tgt tgc act cta ggc atg cta gag aag cag caa   584
Met Gly Met Glu Val Cys Cys Thr Leu Gly Met Leu Glu Lys Gln Gln
            165                 170                 175
 
gct gag gag ctt aag aaa gca ggg ctt acg gcg tat aat cac aat ctt   632
Ala Glu Glu Leu Lys Lys Ala Gly Leu Thr Ala Tyr Asn His Asn Leu
        180                 185                 190
 
gac act tca aga gag tat tat ccc aac att ata acc aca aga tca ttt   680
Asp Thr Ser Arg Glu Tyr Tyr Pro Asn Ile Ile Thr Thr Arg Ser Phe
    195                 200                 205
 
gat gaa cgg ctg gaa acc ctc caa cat gtt cgt gag gca gga ata agt   728
Asp Glu Arg Leu Glu Thr Leu Gln His Val Arg Glu Ala Gly Ile Ser
210                 215                 220                 225
 
gtc tgc tca ggt gga ata att ggg ctg ggt gaa gca gaa gaa gac cgg   776
Val Cys Ser Gly Gly Ile Ile Gly Leu Gly Glu Ala Glu Glu Asp Arg
                230                 235                 240
 
gtt gga ctc ctg cac act cta gcc acc ctc cct act cat cca gag agc   824
Val Gly Leu Leu His Thr Leu Ala Thr Leu Pro Thr His Pro Glu Ser
            245                 250                 255
 
gta ccc att aat gca ctc gta cca gtt aag ggc act ccc ctc caa gat   872
Val Pro Ile Asn Ala Leu Val Pro Val Lys Gly Thr Pro Leu Gln Asp
        260                 265                 270
 
caa aag cct gtg gag atc tgg gag atg atc agg atg atc gca acg gcg   920
Gln Lys Pro Val Glu Ile Trp Glu Met Ile Arg Met Ile Ala Thr Ala
    275                 280                 285
 
cgc atc gtg atg cca caa gca atg gtg cgg ctc tca gca ggc cga gtt   968
Arg Ile Val Met Pro Gln Ala Met Val Arg Leu Ser Ala Gly Arg Val
290                 295                 300                 305
 
cgc ttc tcc atg ccc gag cag gcc ctt tgc ttc ctc gca ggc gcc aac   1016
Arg Phe Ser Met Pro Glu Gln Ala Leu Cys Phe Leu Ala Gly Ala Asn
                310                 315                 320
 
tcc atc ttc acc gga gaa aag ctt ctc acc act gcc aac aac gac ttt   1064
Ser Ile Phe Thr Gly Glu Lys Leu Leu Thr Thr Ala Asn Asn Asp Phe
            325                 330                 335
 
gac gca gat cag ctc atg ttc aaa gtt ctc ggt ctc att ccc aag gca   1112
Asp Ala Asp Gln Leu Met Phe Lys Val Leu Gly Leu Ile Pro Lys Ala
        340                 345                 350
 
cct agc ttt tct gag gag ttt cag gag acg gcg gca gag agc cct gag   1160
Pro Ser Phe Ser Glu Glu Phe Gln Glu Thr Ala Ala Glu Ser Pro Glu
    355                 360                 365
 
ctg gcg gcg gtt tca agt tcc ggt tga attctccgag ctagcattaa         1207
Leu Ala Ala Val Ser Ser Ser Gly  
370                 375
 
gtatttgagc ctcagaacaa aggcggtaat tagtacttga ggtgagctta tatgaggga  1266

<210> 23
<211> 1134
<212> DNA
<213> Lemna minor

<220> 
<221> CDS            
<222> (1)...(1134)
<223> Encodes biotin synthase (BS) isoform #2

<400> 23
atg ctg ttg atc cgg tct ctc cga gcg cga gtc cat cgc tcg tcc tcg   48
Met Leu Leu Ile Arg Ser Leu Arg Ala Arg Val His Arg Ser Ser Ser
 1               5                   10                  15
 
agc ttc gcc ttc tcc acg gct gcc gca tcg gcg gcg act gtg cag gcg   96
Ser Phe Ala Phe Ser Thr Ala Ala Ala Ser Ala Ala Thr Val Gln Ala
             20                  25                  30
 
gaa cga acg ata agg gat ggg ccg agg act gat tgg agc aag gac gag   144
Glu Arg Thr Ile Arg Asp Gly Pro Arg Thr Asp Trp Ser Lys Asp Glu
         35                  40                  45
 
gtc aaa gcg gtt tac gat tct ccc gtc ctc gat ctc ctt ttc cat ggc   192
Val Lys Ala Val Tyr Asp Ser Pro Val Leu Asp Leu Leu Phe His Gly
     50                  55                  60
 
gcc caa gtc cac agg cac gtg cac aag ttc agg gaa gtg caa cag tgt   240
Ala Gln Val His Arg His Val His Lys Phe Arg Glu Val Gln Gln Cys
 65                  70                  75                  80
 
act ctt ctc tcc atc aag aca ggt ggg tgc agc gaa gat tgt tca tat   288
Thr Leu Leu Ser Ile Lys Thr Gly Gly Cys Ser Glu Asp Cys Ser Tyr
                 85                  90                  95
 
tgc ccg caa tcg tct cgc tat gat acg ggg ttg aaa gct caa agg ctc   336
Cys Pro Gln Ser Ser Arg Tyr Asp Thr Gly Leu Lys Ala Gln Arg Leu
            100                 105                 110
 
atg acc aag gat gat gtt ctg gaa gca gca aaa aag gca aaa gat gct   384
Met Thr Lys Asp Asp Val Leu Glu Ala Ala Lys Lys Ala Lys Asp Ala
        115                 120                 125
 
ggc agc aca cgt ttc tgc atg ggg gct gca tgg cgg gat aca att ggc   432
Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Arg Asp Thr Ile Gly
    130                 135                 140
 
cgg aaa acc aac ttc aac cag att ctc aat tac gtc aaa gaa att agg   480
Arg Lys Thr Asn Phe Asn Gln Ile Leu Asn Tyr Val Lys Glu Ile Arg
145                 150                 155                 160
 
gag atg ggc atg gag gtg tgt tgc act cta ggc atg cta gag aag cag   528
Glu Met Gly Met Glu Val Cys Cys Thr Leu Gly Met Leu Glu Lys Gln
                165                 170                 175
 
caa gct gag gag ctt aag aaa gca ggg ctt acg gcg tat aat cac aat   576
Gln Ala Glu Glu Leu Lys Lys Ala Gly Leu Thr Ala Tyr Asn His Asn
            180                 185                 190
 
ctt gac act tca aga gag tat tat ccc aac att ata acc aca aga tca   624
Leu Asp Thr Ser Arg Glu Tyr Tyr Pro Asn Ile Ile Thr Thr Arg Ser
        195                 200                 205
 
ttt gat gaa cgg ctg gaa acc ctc caa cat gtt cgt gag gca gga ata   672
Phe Asp Glu Arg Leu Glu Thr Leu Gln His Val Arg Glu Ala Gly Ile
    210                 215                 220
 
agt gtc tgc tca ggt gga ata att ggg ctg ggt gaa gca gaa gaa gac   720
Ser Val Cys Ser Gly Gly Ile Ile Gly Leu Gly Glu Ala Glu Glu Asp
225                 230                 235                 240
 
cgg gtt gga ctc ctg cac act cta gcc acc ctc cct act cat cca gag   768
Arg Val Gly Leu Leu His Thr Leu Ala Thr Leu Pro Thr His Pro Glu
                245                 250                 255
 
agc gta ccc att aat gca ctc gta cca gtt aag ggc act ccc ctc caa   816
Ser Val Pro Ile Asn Ala Leu Val Pro Val Lys Gly Thr Pro Leu Gln
            260                 265                 270
 
gat caa aag cct gtg gag atc tgg gag atg atc agg atg atc gca acg   864
Asp Gln Lys Pro Val Glu Ile Trp Glu Met Ile Arg Met Ile Ala Thr
        275                 280                 285
 
gcg cgc atc gtg atg cca caa gca atg gtg cgg ctc tca gca ggc cga   912
Ala Arg Ile Val Met Pro Gln Ala Met Val Arg Leu Ser Ala Gly Arg
    290                 295                 300
 
gtt cgc ttc tcc atg ccc gag cag gcc ctt tgc ttc ctc gca ggc gcc   960
Val Arg Phe Ser Met Pro Glu Gln Ala Leu Cys Phe Leu Ala Gly Ala
305                 310                 315                 320
 
aac tcc atc ttc acc gga gaa aag ctt ctc acc act gcc aac aac gac   1008
Asn Ser Ile Phe Thr Gly Glu Lys Leu Leu Thr Thr Ala Asn Asn Asp
                325                 330                 335
 
ttt gac gca gat cag ctc atg ttc aaa gtt ctc ggt ctc att ccc aag   1056
Phe Asp Ala Asp Gln Leu Met Phe Lys Val Leu Gly Leu Ile Pro Lys
            340                 345                 350
 
gca cct agc ttt tct gag gag ttt cag gag acg gcg gca gag agc cct   1104
Ala Pro Ser Phe Ser Glu Glu Phe Gln Glu Thr Ala Ala Glu Ser Pro
        355                 360                 365
 
gag ctg gcg gcg gtt tca agt tcc ggt tga                           1134
Glu Leu Ala Ala Val Ser Ser Ser Gly  
    370                 375
 

<210> 24
<211> 377
<212> PRT
<213> Lemna minor

<400> 24
Met Leu Leu Ile Arg Ser Leu Arg Ala Arg Val His Arg Ser Ser Ser
 1               5                  10                  15      
Ser Phe Ala Phe Ser Thr Ala Ala Ala Ser Ala Ala Thr Val Gln Ala
            20                  25                  30          
Glu Arg Thr Ile Arg Asp Gly Pro Arg Thr Asp Trp Ser Lys Asp Glu
        35                  40                  45              
Val Lys Ala Val Tyr Asp Ser Pro Val Leu Asp Leu Leu Phe His Gly
    50                  55                  60                  
Ala Gln Val His Arg His Val His Lys Phe Arg Glu Val Gln Gln Cys
65                  70                  75                  80  
Thr Leu Leu Ser Ile Lys Thr Gly Gly Cys Ser Glu Asp Cys Ser Tyr
                85                  90                  95      
Cys Pro Gln Ser Ser Arg Tyr Asp Thr Gly Leu Lys Ala Gln Arg Leu
            100                 105                 110         
Met Thr Lys Asp Asp Val Leu Glu Ala Ala Lys Lys Ala Lys Asp Ala
        115                 120                 125             
Gly Ser Thr Arg Phe Cys Met Gly Ala Ala Trp Arg Asp Thr Ile Gly
    130                 135                 140                 
Arg Lys Thr Asn Phe Asn Gln Ile Leu Asn Tyr Val Lys Glu Ile Arg
145                 150                 155                 160 
Glu Met Gly Met Glu Val Cys Cys Thr Leu Gly Met Leu Glu Lys Gln
                165                 170                 175     
Gln Ala Glu Glu Leu Lys Lys Ala Gly Leu Thr Ala Tyr Asn His Asn
            180                 185                 190         
Leu Asp Thr Ser Arg Glu Tyr Tyr Pro Asn Ile Ile Thr Thr Arg Ser
        195                 200                 205             
Phe Asp Glu Arg Leu Glu Thr Leu Gln His Val Arg Glu Ala Gly Ile
    210                 215                 220                 
Ser Val Cys Ser Gly Gly Ile Ile Gly Leu Gly Glu Ala Glu Glu Asp
225                 230                 235                 240 
Arg Val Gly Leu Leu His Thr Leu Ala Thr Leu Pro Thr His Pro Glu
                245                 250                 255     
Ser Val Pro Ile Asn Ala Leu Val Pro Val Lys Gly Thr Pro Leu Gln
            260                 265                 270         
Asp Gln Lys Pro Val Glu Ile Trp Glu Met Ile Arg Met Ile Ala Thr
        275                 280                 285             
Ala Arg Ile Val Met Pro Gln Ala Met Val Arg Leu Ser Ala Gly Arg
    290                 295                 300                 
Val Arg Phe Ser Met Pro Glu Gln Ala Leu Cys Phe Leu Ala Gly Ala
305                 310                 315                 320 
Asn Ser Ile Phe Thr Gly Glu Lys Leu Leu Thr Thr Ala Asn Asn Asp
                325                 330                 335     
Phe Asp Ala Asp Gln Leu Met Phe Lys Val Leu Gly Leu Ile Pro Lys
            340                 345                 350         
Ala Pro Ser Phe Ser Glu Glu Phe Gln Glu Thr Ala Ala Glu Ser Pro
        355                 360                 365             
Glu Leu Ala Ala Val Ser Ser Ser Gly
    370                 375         


<210> 25
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<220> 
<221> misc_feature   
<222> (19)...(19)
<223> t/u or c

<220> 
<221> misc_feature   
<222> (22)...(22)
<223> g or a

<220> 
<221> misc_feature   
<222> (23)...(23)
<223> t/u or c

<220> 
<221> misc_feature   
<222> (25)...(25)
<223> a or g or c or t


<220> 
<221> misc_feature   
<222> (26)...(26)
<223> a or c

<400> 25
gcagcccgtg ttctccttya arytnmg                                     27

<210> 26
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<220> 
<221> misc_feature   
<222> (11)...(11)
<223> a or t/u

<220> 
<221> misc_feature   
<222> (21)...(21)
<223> a or g or c or t

<220> 
<221> misc_feature   
<222> (22)...(22)
<223> t/u or c

<220> 
<221> misc_feature   
<222> (23)...(23)
<223> g or t/u


<220> 
<221> misc_feature   
<222> (24)...(24)
<223> a or g or c or t

<400> 26
tggaagaggg wgatgttcca nykngg                                      26

<210> 27
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<220> 
<221> misc_feature   
<222> (14)...(14)
<223> t/u or c

<220> 
<221> misc_feature   
<222> (17)...(17)
<223> a or g or c or t

<220> 
<221> misc_feature   
<222> (20)...(20)
<223> go or a

<400> 27
ccgccggcaa ccaygcncar gg                                          22

<210> 28
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<220> 
<221> misc_feature   
<222> (18)...(18)
<223> t/u or c

<220> 
<221> misc_feature   
<222> (22)...(22)
<223> g or a

<220> 
<221> misc_feature   
<222> (25)...(25)
<223> a or g or c or t

<220> 
<221> misc_feature   
<222> (28)...(28)
<223> a or g or c or t

<400> 28
gtgcagcttg tcgaagtyca trttngcncc                                  30

<210> 29
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 29
ctcggcatag gcgataagt                                              19

<210> 30
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 30
gaggcccgat tcatgccat                                              19

<210> 31
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 31
gcggaatgaa agttcggc                                               18

<210> 32
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 32
agtatcctcg agccagcc                                               18

<210> 33
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 33
ctctcggatc ctgcatcgtc tt                                          22

<210> 34
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 34
cagaagccat aacaccgcat aca                                         23

<210> 35
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 35
tatgtcgaca tgaaggtcac caccgact                                    28

<210> 36
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 36
ttctagacaa aattttcaaa ccccatg                                     27

<210> 37
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 37
ttctagacgc catggcattt gcatcgt                                     27

<210> 38
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 38
tgagctcatg aaggtcacca ccgactc                                     27

<210> 39
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> Primer

<400> 39
tgccctagag atgtccaaca agg                                         23

<210> 40
<211> 2011
<212> DNA
<213> Spirodela polyrrhiza

<400> 40
gatggacaga taatgagatg aattagaaaa aaaaaattcg tgttgtaaga tagaatactt 60
gctatctact gatgaatgca gttcagtttt cctcacgatc ttaaagatcg cgcactatcc 120
tcagcttcac tctggaaatt ttgattctct tcttctgctc agcagcctcg actctgtcta 180
gggtttcgta caatcggacg ccattctaca tgaatcgagc acagggaatg aagacaatta 240
ggagatcctc gatgtcctcc gacttacttg catgacttga cggggaagat ctcgagcagg 300
gaagcgacgc ctctccggag gactcgcctc gccgagagga cctcctccgc gacacggacc 360
atggcctcca cggggtagaa gctggccctg ttctttattc tcttgaggat catcggccga 420
agcctccgca aatccatccc cgaggagtag aatctcgcct gcaggaagca tctgtcgaga 480
tcctcgccga ggcggcggag atacctcgcc ggcgccgcca tggcgccggg gacggagcac 540
caccacggag aagaagaacc ctaacccaag gcattaacga agttgcgcag attatacaaa 600
agccctcaaa tatctttcat tttctatttc actgatacat tttcattatt gtatatgagt 660
gtttatttaa attattccgt attagaaaag cacctccaga acccgacaaa atagggtgac 720
gtcatcatgg tgtcatgacc gcccaacagc cgcagattta aaatcggtgg atgagtgcgg 780
ccacgccacg aaagcgatgg gccttcgtcg atgccgtgag aatccatctg acataaagta 840
aacggcgccg tcagtattga cggcgtatga cacgtggaaa gaagctattg gttcacgcat 900
cggtggttcc gctagcctcc gtcgaccgct agtactataa atacggtccc gaggcctcct 960
caccactcgc acatatcctc tttgttttcc tctccgtgaa agaagcgagg aagcgcgtcg 1020
tctctcccaa ggtaaggagc agatctcttt gatcgttttt gttcttcttt tgttttgttt 1080
tttttttctg cggatcttcg gttgcatcat gccttggctg tttttattag tttaggatat 1140
cctcgtttgg atctgagccg atcatatatg ttaaaggttg tgttcgatct ctttgttcat 1200
tttcgcatga aaaggatgta tccttttgat gtgaggcgat cttctatggt taagactttg 1260
ttcggtctat tgatcatttc tgttcttcgt ttttgagttt ttttctgcgg atatcgcatc 1320
atccctaggt ttttgctttg gttaggatgc atcctttgga tttgagccga tctcccttgg 1380
ttaaggctgt gtctgttgca gaggagaaag tctgtcgagg tccttatgca ggctttgtcc 1440
agatgcgcgt gctctctcat gctatgaatt tatgttttga gaactcctcc cggtttttct 1500
agatccggat ttgaagtatt cattgcggtt ccccttcggt tttatgtatt tctcgagttg 1560
atttggtcca tgatcgtgtt ctgtccagat ctctcttgat atggatgaga tattcgttac 1620
ctctttcaaa catcggtgga tgttcttttt agtcttggct cacctttatc tagaaattaa 1680
ttttcggttt gaaacccctg cttgttaagg tgatgtattc cttctttata gatttcggtg 1740
tgttatttct taacggtgat ctgtccgatc catgtgttgc acctcttgtt ttctgtgtaa 1800
tcctctgtga attataatta tgttttgaaa acgtacttaa gtaaggggca tgttccccgt 1860
ttaaaacttt tgttctatca atttgtggtt aatagatcct gatttgtggt cgccttattc 1920
tgtctttaat cgtggatttt atttatcttg agcgcgtcct tttcttttaa aatcatgtgt 1980
ttaacctttc agtcgtcata tgttccatca g                                2011

<210> 41
<211> 1142
<212> DNA
<213> Artificial Sequence

<220> 
<223> Truncated SpUbq117 promoter

<400> 41
acacgtggaa agaagctatt ggttcacgca tcggtggttc cgctagcctc cgtccaccgc 60
tagtactata aatacggtcc cgaggcctcc tcaccactcg cacatatcct ctttgttttc 120
ctctccgtga aagaagcgag gaagcgcgtc gtctctccca aggtaaggag cagatctctt 180
tgatcgtttt tgttcttctt ttgttttgtt ttttttttct gcggatcttc ggttgcatca 240
tgccttggct gtttttatta gtttaggata tcctcgtttg gatctgagcc gatcatatat 300
gttaaaggtt gtgttcgatc tctttgttca ttttcgcrtg aaaaggatgt atccttttga 360
tgtgaggcga tcttctatgg ttaagacttt gttcggtcta ttgatcattt ctgttcttcg 420
tttttgagtt tttttctgcg gatatcgcat catccctagg tttttgcttt ggttaggatg 480
catcctttgg atttgagccg atctcccttg gttaaggctg tgtctgttgc agaggagaaa 540
gtctgtcgag gtccttatgc aggctttgtc cagatgcgcg tgctctctca tgctatgaat 600
ttatgttttg agaactcctc ccggtttttc tagatccgga tttgaagtat tcattgcggt 660
tccccttcgg ttttatgtat ttctcgagtt gatttggtcc atgatcgtgt tctgtccaga 720
tctctcttga tatggatgag atattcgtta cctctttcaa acatcggtgg atgttctttt 780
tagtcttggc tcacctttat ctagaaatta attttcggtt tgaaacccct gcttgttaag 840
gtgatgtatt ccttctttat agatttcggt gtgttatttc ttaacggtga tctgtccgat 900
ccatgtgttg cacctcttgt tttctgtgta atcctctgtg aattataatt atgttttgaa 960
aacgtactta agtaaggggc atgttccccg tttaaaactt ttgttctatc aatttgtggt 1020
taatagatcc tgatttgtgg tcgccttatt ctgtctttaa tcgtggattt tatttatctt 1080
gagcgcgtcc ttttctttta aaatcatgtg tttaaccttt cagtcgtcat atgttccatc 1140
ag                                                                1142
