                         SEQUENCE LISTING

<110>  Scil Proteins GmbH
 
<120>  DIMERIC BINDING PROTEINS BASED ON MODIFIED UBIQUITINS

<130>  P31550-WO

<150>  EP 11 170 054.8

<151>  15. Juni 2011

<160>  18    

<170>  PatentIn version 3.3

<210>  1
<211>  76
<212>  PRT
<213>  artificial

<220>
<223>  wt ubiquitin

<400>  1

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  2
<211>  163
<212>  PRT
<213>  artificial

<220>
<223>  dimeric ubiquitin binding protein 40401

<400>  2

Met Gln Ile Phe Val Tyr Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Val Ala 
    50                  55                  60                  


Glu Tyr Leu Gly Ile Ser Trp Met Pro Ala Leu His Leu Val Leu Arg 
65                  70                  75                  80  


Leu Arg Gly Gly Gly Ile Gly Met Gln Ile Phe Val Ala Thr Asp Thr 
                85                  90                  95      


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
            100                 105                 110         


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln 
        115                 120                 125             


Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser 
    130                 135                 140                 


Asp Tyr Asn Ile Arg Asp Thr Val Ser Leu His Leu Val Leu Arg Leu 
145                 150                 155                 160 


Arg Ala Ala 
            


<210>  3
<211>  163
<212>  PRT
<213>  artificial

<220>
<223>  dimeric ubiquitin binding protein 59517

<400>  3

Met Gln Ile Phe Val Tyr Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Val Ala 
    50                  55                  60                  


Glu Tyr Leu Gly Ile Ser Trp Met Pro Ala Leu His Leu Val Leu Arg 
65                  70                  75                  80  


Leu Arg Ala Ala Gly Ile Gly Met Gln Ile Phe Val Leu Thr Ser Thr 
                85                  90                  95      


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
            100                 105                 110         


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln 
        115                 120                 125             


Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser 
    130                 135                 140                 


Asp Tyr Asn Ile Thr Arg Asn Tyr His Leu His Leu Val Leu Arg Leu 
145                 150                 155                 160 


Arg Ala Ala 
            


<210>  4
<211>  163
<212>  PRT
<213>  artificial

<220>
<223>  dimeric ubiquitin binding protein 59649

<400>  4

Met Gln Ile Phe Val Tyr Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Val Ala 
    50                  55                  60                  


Glu Tyr Leu Gly Ile Ser Trp Met Pro Ala Leu His Leu Val Leu Arg 
65                  70                  75                  80  


Leu Arg Ala Ala Gly Ile Gly Met Gln Ile Phe Val Leu Thr Arg Thr 
                85                  90                  95      


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
            100                 105                 110         


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln 
        115                 120                 125             


Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser 
    130                 135                 140                 


Asp Tyr Asn Ile Thr Ser Lys Ser Ser Leu His Leu Val Leu Arg Leu 
145                 150                 155                 160 


Arg Ala Ala 
            


<210>  5
<211>  163
<212>  PRT
<213>  artificial

<220>
<223>  dimeric ubiquitin binding protein 60423

<400>  5

Met Gln Ile Phe Val Tyr Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Val Ala 
    50                  55                  60                  


Glu Tyr Leu Gly Ile Ser Trp Met Pro Ala Leu His Leu Val Leu Arg 
65                  70                  75                  80  


Leu Arg Ala Ala Gly Ile Gly Met Gln Ile Phe Val Arg Thr Arg Thr 
                85                  90                  95      


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
            100                 105                 110         


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln 
        115                 120                 125             


Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser 
    130                 135                 140                 


Asp Tyr Asn Ile Gln Asn Gln Phe Gln Leu His Leu Val Leu Arg Leu 
145                 150                 155                 160 


Arg Ala Ala 
            


<210>  6
<211>  163
<212>  PRT
<213>  artificial

<220>
<223>  dimeric ubiquitin binding protein 60323

<400>  6

Met Gln Ile Phe Val Tyr Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Val Ala 
    50                  55                  60                  


Glu Tyr Leu Gly Ile Ser Trp Met Pro Ala Leu His Leu Val Leu Arg 
65                  70                  75                  80  


Leu Arg Ala Ala Gly Ile Gly Met Gln Ile Phe Val Asp Thr Glu Thr 
                85                  90                  95      


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
            100                 105                 110         


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln 
        115                 120                 125             


Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser 
    130                 135                 140                 


Asp Tyr Asn Ile Glu Gln Leu Asn Trp Leu His Leu Val Leu Arg Leu 
145                 150                 155                 160 


Arg Ala Ala 
            


<210>  7
<211>  163
<212>  PRT
<213>  artificial

<220>
<223>  dimeric ubiquitin binding protein 60397

<400>  7

Met Gln Ile Phe Val Tyr Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Val Ala 
    50                  55                  60                  


Glu Tyr Leu Gly Ile Ser Trp Met Pro Ala Leu His Leu Val Leu Arg 
65                  70                  75                  80  


Leu Arg Ala Ala Gly Ile Gly Met Gln Ile Phe Val Ala Thr Asp Thr 
                85                  90                  95      


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
            100                 105                 110         


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln 
        115                 120                 125             


Arg Leu Ile Trp Ala Gly Lys Gln Leu Lys Asp Gly Arg Thr Leu Ser 
    130                 135                 140                 


Asp Tyr Asn Ile Asn Asp Glu Ser Ala Leu His Leu Val Leu Arg Leu 
145                 150                 155                 160 


Arg Ala Ala 
            


<210>  8
<211>  163
<212>  PRT
<213>  artificial

<220>
<223>  dimeric ubiquitin binding protein 59507

<400>  8

Met Gln Ile Phe Val Tyr Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Ser Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Val Ala 
    50                  55                  60                  


Glu Tyr Leu Gly Ile Ser Trp Met Pro Ala Leu His Leu Val Leu Arg 
65                  70                  75                  80  


Leu Arg Ala Ala Gly Ile Gly Met Gln Ile Phe Val Ser Thr Phe Thr 
                85                  90                  95      


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
            100                 105                 110         


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln 
        115                 120                 125             


Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser 
    130                 135                 140                 


Asp Tyr Asn Ile Ile Asp Trp Ser Gln Leu His Leu Val Leu Arg Leu 
145                 150                 155                 160 


Arg Ala Ala 
            


<210>  9
<211>  163
<212>  PRT
<213>  artificial

<220>
<223>  dimeric ubiquitin binding protein 59987

<400>  9

Met Gln Ile Phe Val Tyr Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Val Ala 
    50                  55                  60                  


Glu Tyr Leu Gly Ile Ser Trp Met Pro Ala Leu His Leu Val Leu Arg 
65                  70                  75                  80  


Leu Arg Ala Ala Gly Ile Gly Met Gln Ile Phe Val Ser Thr Arg Thr 
                85                  90                  95      


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
            100                 105                 110         


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln 
        115                 120                 125             


Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser 
    130                 135                 140                 


Asp Tyr Asn Ile Arg Lys His Tyr Thr Leu His Leu Val Leu Arg Leu 
145                 150                 155                 160 


Arg Ala Ala 
            


<210>  10
<211>  163
<212>  PRT
<213>  artificial

<220>
<223>  dimeric ubiquitin binding protein 59603

<400>  10

Met Gln Ile Phe Val Tyr Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Glu 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Val Ala 
    50                  55                  60                  


Glu Tyr Leu Gly Ile Ser Trp Met Pro Ala Leu His Leu Val Leu Arg 
65                  70                  75                  80  


Leu Arg Ala Ala Gly Ile Gly Met Arg Ile Phe Val Tyr Thr Ala Thr 
                85                  90                  95      


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
            100                 105                 110         


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln 
        115                 120                 125             


Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser 
    130                 135                 140                 


Asp Tyr Asn Ile Ser Glu Lys Lys Lys Leu His Leu Val Leu Arg Leu 
145                 150                 155                 160 


Arg Ala Ala 
            


<210>  11
<211>  160
<212>  PRT
<213>  artificial

<220>
<223>  dimeric ubiquitin protein sequence


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (62)..(74)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (90)..(90)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (92)..(92)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (146)..(150)
<223>  Xaa can be any naturally occurring amino acid

<400>  11

Met Gln Ile Phe Val Xaa Thr Xaa Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Xaa Xaa Xaa 
    50                  55                  60                  


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Leu His Leu Val Leu Arg 
65                  70                  75                  80  


Leu Arg Asn Asn Met Gln Ile Phe Val Xaa Thr Xaa Thr Gly Lys Thr 
                85                  90                  95      


Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala 
            100                 105                 110         


Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile 
        115                 120                 125             


Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn 
    130                 135                 140                 


Ile Xaa Xaa Xaa Xaa Xaa Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155                 160 


<210>  12
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  insert

<400>  12

Asp Val Ala Glu Tyr Leu Gly Ile 
1               5               


<210>  13
<211>  232
<212>  PRT
<213>  artificial

<220>
<223>  VEGF-A (Accession number P15692)

<400>  13

Met Asn Phe Leu Leu Ser Trp Val His Trp Ser Leu Ala Leu Leu Leu 
1               5                   10                  15      


Tyr Leu His His Ala Lys Trp Ser Gln Ala Ala Pro Met Ala Glu Gly 
            20                  25                  30          


Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr Gln 
        35                  40                  45              


Arg Ser Tyr Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln Glu 
    50                  55                  60                  


Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro Leu 
65                  70                  75                  80  


Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val Pro 
                85                  90                  95      


Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Arg Ile Lys Pro His 
            100                 105                 110         


Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys Cys 
        115                 120                 125             


Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Lys Ser Val 
    130                 135                 140                 


Arg Gly Lys Gly Lys Gly Gln Lys Arg Lys Arg Lys Lys Ser Arg Tyr 
145                 150                 155                 160 


Lys Ser Trp Ser Val Tyr Val Gly Ala Arg Cys Cys Leu Met Pro Trp 
                165                 170                 175     


Ser Leu Pro Gly Pro His Pro Cys Gly Pro Cys Ser Glu Arg Arg Lys 
            180                 185                 190         


His Leu Phe Val Gln Asp Pro Gln Thr Cys Lys Cys Ser Cys Lys Asn 
        195                 200                 205             


Thr Asp Ser Arg Cys Lys Ala Arg Gln Leu Glu Leu Asn Glu Arg Thr 
    210                 215                 220                 


Cys Arg Cys Asp Lys Pro Arg Arg 
225                 230         


<210>  14
<211>  3
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  14

Gly Ile Gly 
1           


<210>  15
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  15

Ser Gly Gly Gly Gly 
1               5   


<210>  16
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  16

Ser Gly Gly Gly Gly Ile Gly 
1               5           


<210>  17
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  17

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ile Gly 
1               5                   10          


<210>  18
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  18

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10  


