                         SEQUENCE LISTING

<110>  FUNDACION CENTRO NACIONAL DE INVESTIGACIONES ONCOLOGICAS 
       CARLOS III
       CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
 
<120>  RPAP1

<130>  PCT-677

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  58
<212>  RNA
<213>  Artificial sequence

<220>
<223>  hsRNA shRPAP1: hairpin sequence TRCN0000173186, which target mRNA
       of Rpap1 gene of Mus musculus


<220>
<221>  misc_binding
<222>  (5)..(25)
<223>  Target sequence for mRNA: Position +3838 of mRNA with reference 
       NM_177294

<220>
<221>  misc_feature
<222>  (26)..(31)
<223>  Loop of shRNA

<220>
<221>  misc_feature
<222>  (32)..(52)
<223>  Reverse target sequence

<400>  1
ccggccaagu cuugccaaac cucaacucga guugagauuu ggcaagacuu gguuuuug         58


<210>  2
<211>  21
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_binding
<222>  (1)..(21)
<223>  Target sequence of shRNA of SEQ ID NO:1 in rpap1 gene \note: 
       match position on target mRNA of reference NM_177294:+3838

<400>  2
ccaagtcttg ccaaacctca a                                                 21


<210>  3
<211>  58
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward Oligo Sequence matching the coding sequence of shRPAP1 of
       vector pLKO.1 of Open Biosystems

<400>  3
ccggccaagt cttgccaaac ctcaactcga gttgaggttt ggcaagactt ggtttttg         58


<210>  4
<211>  58
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse Oligo Sequence matching the coding sequence of shRPAP1 of
       vector pLKO.1 of Open Biosystems

<400>  4
aattcaaaaa ccaagtcttg ccaaacctca actcgagttg aggtttggca agacttgg         58


<210>  5
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: forward primer for GAPDH for quantitative real-time PCR  
       \source: Mus musculus

<400>  5
ttcaccacca tggagaaggc                                                   20


<210>  6
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for GAPDH for quantitative real-time PCR  
       \source: Mus musculus

<400>  6
cccttttggc tccaccct                                                     18


<210>  7
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for beta-actin for quantitative real-time 
       PCR                                              \source: Mus 
       musculus

<400>  7
ggcaccacac cttctacaat g                                                 21


<210>  8
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for beta actin for quantitative real-time 
       PCR                                           \source: Mus 
       musculus

<400>  8
gtggtggtga agctgtagcc                                                   20


<210>  9
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for endo-Oct4 for quantitative real-time 
       PCR                                             \source: Mus 
       musculus

<400>  9
tctttccacc aggcccccgg ctc                                               23


<210>  10
<211>  23
<212>  DNA
<213>  Artificial sequence;Primer:Reverse primer for quantitative real-time PCR

<220>
<223>  Primer: Reverse primer for Endo_Oct4 for quantitative real-time 
       PCR                                         \source: Mus musculus

<400>  10
tgcgggcgga catggggaga tcc                                               23


<210>  11
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for Endo-Sox2 for quantitative real-time 
       PCR                                          \source: Mus 
       musculus

<400>  11
tagagctaga ctccgggcga tga                                               23


<210>  12
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for Endo-sox2 for quantitative real-time 
       PCR                                           \source: Mus 
       musculus

<400>  12
ttgccttaaa caagaccacg aaa                                               23


<210>  13
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for Endo-Klf4 for quantitative real-time 
       PCR                                              \source: Mus 
       musculus

<400>  13
gcgaactcac acaggcgaga aacc                                              24


<210>  14
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for Endo-Klf4 for quantitative real-time 
       PCR                                      \source: Mus musculus

<400>  14
tcgcttcctc ttcctccgac aca                                               23


<210>  15
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for Nanog for quantitative real-time PCR  
       \source: Mus musculus

<400>  15
caggtgtttg agggtagctc                                                   20


<210>  16
<211>  77
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for Nanog for quantitative real-time PCR  
       \source: Mus musculus


<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (46)..(46)
<223>  n is a, c, g, or t

<400>  16
cggttcatca tggtacagtc radnaartca sncrmrrvrs rmrranttat vratmcrcac       60

ccttctctgc ctgggcc                                                      77


<210>  17
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for RPAP1 for quantitative real-time PCR  
       \source: Mus musculus

<400>  17
cacccttctc tgcctgggcc                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for RPAP1 for quantitative real-time PCR  
       \source: Mus musculus

<400>  18
tagcagctgc ggatgctggg                                                   20


<210>  19
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for E-Ras for quantitative real-time PCR  
       \source: Mus musculus

<400>  19
ccctcatcag actgctactc ctgg                                              24


<210>  20
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for E-Ras for quantitative real-time PCR  
       \source: Mus musculus

<400>  20
cacgcagagc ccggtgaata tcct                                              24


<210>  21
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for E-cadherin for quantitative real-time 
       PCR                                           \source: Mus 
       musculus

<400>  21
ttttcggaag actcccgatt ca                                                22


<210>  22
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for E-cadherin for quantitative real-time 
       PCR                                           \source: Mus 
       musculus

<400>  22
agcttgtgga gctttagatg c                                                 21


<210>  23
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for N-cadherin for quantitative real-time 
       PCR                                           \source: Mus 
       musculus

<400>  23
ctgatagccc ggtttcactt g                                                 21


<210>  24
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for N-cadherin for quantitative real-time 
       PCR                                          \source: Mus 
       musculus

<400>  24
caggctttga tccctctgga                                                   20


<210>  25
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for Snail for quantitative real-time PCR  
       \source: Mus musculus

<400>  25
cacacgctgc cttgtgtct                                                    19


<210>  26
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for Snail for quantitative real-time PCR  
       \source: Mus musculus

<400>  26
ggtcagcaaa agcacggtt                                                    19


<210>  27
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for Slug for quantitative real-time PCR   
       \source: Mus musculus

<400>  27
tggtcaagaa acatttcaac gcc                                               23


<210>  28
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for Slug for quantitative real-time PCR   
       \source: Mus musculus

<400>  28
ggtgaggatc tctggttttg gta                                               23


<210>  29
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for HoxA1 for quantitative real-time PCR  
       \source: Mus musculus

<400>  29
acttcagtgc gccttatggc                                                   20


<210>  30
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for HoxA1 for quantitative real-time PCR  
       \source: Mus musculus

<400>  30
atgggagtcg agaggtttcc a                                                 21


<210>  31
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for HoxB1 for quantitative real-time PCR  
       \source: Mus musculus

<400>  31
cccttccaac tcagttcagt gcct                                              24


<210>  32
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reserve primer for HoxB1 for quantitative real-time PCR  
       \source: Mus musculus

<400>  32
ttggtggcga ttgggctcac actc                                              24


<210>  33
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for Myo-D1 for quantitative real-time PCR 
       \source: Mus musculus

<400>  33
ccactccggg acatagactt g                                                 21


<210>  34
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer Myo-D1 for quantitative real-time PCR     
       \source: Mus musculus

<400>  34
aaaagcgcag gtctggtgag                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for Fgf5 for quantitative real-time PCR   
       \source: Mus musculus

<400>  35
tgtactgcag agtgggcatc                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reserve primer for Fgf5 for quantitative real-time PCR   
       \source: Mus musculus

<400>  36
acaatcccct gagacacagc                                                   20


<210>  37
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for Cyp26a1 for quantitative real-time PCR
       \source: Mus musculus

<400>  37
cccgtgatcg ctgaggaag                                                    19


<210>  38
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for Cyp26a1 for quantitative real-time PCR
       \source: Mus musculus

<400>  38
gggcacgtca atgggaagag                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for Cardiac alpha-actinin for quantitative
       real-time PCR                      \source: Mus musculus

<400>  39
ctggtattgc cgatcgtatg                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for Cardiac alpha-actinin for quantitative
       real-time PCR                     \source: Mus musculus

<400>  40
cttgctgatc cacatttgct                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for ANP - Atrial Natriuretic Peptide for 
       quantitative real-time PCR    \source: Mus musculus

<400>  41
actaggctgc aacagcttcc                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for ANP - Atrial natriuretic Peptide for 
       quantitative real-time PCR    \source: Mus musculus

<400>  42
tgacacacca caagggctta                                                   20


<210>  43
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for Pax3 for quantitative real-time PCR   
       \source: Mus musculus

<400>  43
tttcacctca ggtaatggga ct                                                22


<210>  44
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for Pax3 for quantitative real-time PCR   
       \source: Mus musculus

<400>  44
gaacgtccaa ggcttacttt gt                                                22


<210>  45
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for Zeb1 for quantitative real-time PCR   
       \source: Mus musculus

<400>  45
gctggcaaga caacgtgaaa g                                                 21


<210>  46
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for Zeb1 for quantitative real-time PCR   
       \source: Mus musculus

<400>  46
gcctcaggat aaatgacggc                                                   20


<210>  47
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for Zeb2 for quantitative real-time PCR   
       \source: Mus musculus

<400>  47
caggctcgga gacagatgaa g                                                 21


<210>  48
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for Zeb2 for quantitative real-time PCR   
       \source: Mus musculus

<400>  48
cttgcagaat ctcgccactg                                                   20


<210>  49
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for Twist1 for quantitative real-time PCR 
       \source: Mus musculus

<400>  49
ggacaagctg agcaagattc a                                                 21


<210>  50
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for Twist1 for quantitative real-time PCR 
       \source: Mus musculus

<400>  50
cggagaaggc gtagctgag                                                    19


<210>  51
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Forward primer for P16/Ink4a for quantitative real-time 
       PCR                                            \source: Mus 
       musculus

<400>  51
cgtaccccga ttcaggtgat                                                   20


<210>  52
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Reverse primer for P16/Ink4a for quantitative real-time 
       PCR                                           \source: Mus 
       musculus

<400>  52
ttgagcagaa gagctgctac gt                                                22


