                         SEQUENCE LISTING

<110>  Arizona State University
       Hu, Qiang
       Yoon, Kang Sup
       Sommerfield, Milton
 
<120>  PHOSPHOLIPID:DIACYLGLYCEROL ACETYLTRANSFERASE GENES, PROTEINS, 
       AND USES THEREOF

<130>  055743-442307

<150>  US 61/493,957
<151>  2011-06-06

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  3123
<212>  DNA
<213>  Chlamydomonas reinhardtii

<400>  1
atgacgaccc caacaaaagg caatagcaat gctcgacaac gaaagaaggg aggaaccagt       60

gccgaggcgt ccgcggcgac accgtcaaaa gccaagcctg gaagggacca taacgtgcac      120

gcaaccccca gccactcgca ctcgcactcg cactcccagt ctcagcagca ccggcaagga      180

ccgcatgctg cccaaccaaa atcggagcgg cgtctggtgc tatggctggc ggcggcgggg      240

gtggtgctgc tgccgctggt gctgctgccg cccgcactaa tggtggagga gggcccagtc      300

gctgagcttc tgcacgactc cctgggcctg cctcgcctca atgcctacct accgccgctc      360

ccctccatcg tagccctgga ctcattgctc gcctccgctg gcttcgccgt gccctcctcc      420

gccctcaacg gcaccagcgc cgccgccgcc gccgccaaca gcagcgccta taacggcctg      480

ctggcgctgt tcggcgtcac aggcgacatg gcatcagccg gcatggatgg tctgactagc      540

cgtacagtga cggcggcgca gcagctgcgg gcattcctag cacagctgcc cgcgctgagc      600

atacccggcg gcccggcggc gactggcggg gggcccgctg gagccggtgc cggggcgggt      660

gcgggtgcgg gggcagaggt taatgccacg gcggctgcgg atgaggctgc ggggctgcgg      720

ccgggtcagc tgatggcgcg gcgcggctac cgagccaaac acccggtggt cattgtgcca      780

ggctttgtga cctcgggtct ggagctgtgg cggggcctgc catgtggcca gcgctacttc      840

cgacagcgca tgtgggggac cctggccatg gtgcaggcct tcctgacgga cgcggcgtgt      900

tggttccgcc acatggagct ggacacagtg tcgggcctgg acccggaggg catcaagctg      960

cgcgccgcgc tggggctgga ggcggttgac tatttcatac aaggctactg ggtgtggggc     1020

aagctggtgg aggcgctggc tgacgtgggg tacgacagca acagcctagt cagcatgccg     1080

tacgactggc ggctggccgt gccgctgctg gaggagcgcg atggctacta cacgcggctg     1140

cggcgcacca tcgagcagct tgtggagctg acgggggagc gggtggtggt caccagccac     1200

tcgtacggag agaacgtgtt ccgcgccttc atgcactggg tggaggccgc cgccgcggag     1260

gaggaggagg gcggcaagca gccgcgctcg ggcggcggca gcggccacag cggtggcggc     1320

tgggtggacc gccacatcgc cagcaccatc aacatcgccg gcaccagcct gggcgtgcca     1380

aagtccgtgt ctgcgctgct gtcgggcgag acacgcgaca ccgcccagct cggggccctg     1440

gcgggcttct tgacctccaa catggtcccg cgcgccgccc gcacccgcgt gtggcgcagc     1500

tggggagcct cctacgccat gctgcctgtg ggcgggccgg gcgtgtgggg caacgcctcc     1560

tgggcgcccg acgacacgcc cgagatgcgg gccaaccgcc gcacgttcgg ctccatggtg     1620

tctctgtggc cgcacaactg gcaggcgctc atggccgccg ccccgcagca gcagcgcgag     1680

cagctcagcc agctggtcgc cgaggtcagc caggacgtgg atgcgggcag cggcagcggc     1740

agcggcagcg gcagtggaag cggcagtgga aggggaagcg gaagcggaag cggaagcgga     1800

agcggaagcg gcgaggatgc tgtcaatgga actggcggtg ccggcagtgc cggtggtgca     1860

gcgctagctc cggccgaggc agcggcggtc ggcactgcgg gtggcggcga caatgctacg     1920

gctgccacgg ctgtaaacag caccacagcc agcaacagca ccggctctgc aggcaccggc     1980

agcagtggca gcggcggcag cagcagcagc aaggccattg cggcgattgc ggacacaatt     2040

aagaagctcg ttgggggcgg cgttgatgcc gcagaattcc tcaacagcga ccatgtcaca     2100

cggctggacg tctcggggtt catcgcactg ctgcgagagg tgggcggccc cctggtcagc     2160

gccaacatcg cgcagtgggg cgcactgcag ctgccggcgg aggaggcagc cgaggcggtt     2220

cgtaagcgcg ctgccgctgc ctcagaccgc gccgcccctg ccgccgccgg cagccccgcg     2280

gcacaggcgg tgggcggagc cgacagagtc aaggactcaa ccgctggcgg tgccggcgca     2340

gccgggatcg ggcgtggcgg ctctagcgcg ggtagcgggc gggtggctct gtttcctgac     2400

gccacccgca cgccgctccc tagcgctccc aagacaacaa tggtatgcct ctacggcgtt     2460

ggactgccaa cggagcgcgg gtaccactac ctcaggccac cgccgccgac cggcgccgcg     2520

gccaccgcaa ctgctgcggc cgcgaatgcg ccggcgggca acgccgccgc cgctccggcg     2580

gcggacgcac agccaccgcc gcaggccgct gggggcggtg acggtgacgg catggcgaca     2640

acggacggca ctgcggaggc tgatgcggag aggggggtgc cgccgcgggg cgccaccgca     2700

tccggggagg aggctaaccc ggacgcgggc ggcgatgcct catcgggttg gatgatcatg     2760

aaagacgtca cgcaagcgca gagcgcgttg gacgtggggg tgcacatcag cgacggcgac     2820

ggaacagtgc cgctgctgag cctggggctc atgtgccgcg gcggctggcg ggaggcgggg     2880

cacctcaacc cgggagccat gagagtggtg acccgggagt acaagcacaa agccgtgtcg     2940

atgcttcagg acgcaagggg cggccccgct gccgccgcgc acatcgacat cctgggcaac     3000

gacgcagtgc tgcgggatgt gatcacggtg gtggcgggcc gggcggatga gctggcggac     3060

attgtcgtca gcgacataga ccgcatcgca gccgccgtgg actgggccgc actggccgcc     3120

tga                                                                   3123


<210>  2
<211>  1040
<212>  PRT
<213>  Chlamydomonas reinhardtii

<400>  2

Met Thr Thr Pro Thr Lys Gly Asn Ser Asn Ala Arg Gln Arg Lys Lys 
1               5                   10                  15      


Gly Gly Thr Ser Ala Glu Ala Ser Ala Ala Thr Pro Ser Lys Ala Lys 
            20                  25                  30          


Pro Gly Arg Asp His Asn Val His Ala Thr Pro Ser His Ser His Ser 
        35                  40                  45              


His Ser His Ser Gln Ser Gln Gln His Arg Gln Gly Pro His Ala Ala 
    50                  55                  60                  


Gln Pro Lys Ser Glu Arg Arg Leu Val Leu Trp Leu Ala Ala Ala Gly 
65                  70                  75                  80  


Val Val Leu Leu Pro Leu Val Leu Leu Pro Pro Ala Leu Met Val Glu 
                85                  90                  95      


Glu Gly Pro Val Ala Glu Leu Leu His Asp Ser Leu Gly Leu Pro Arg 
            100                 105                 110         


Leu Asn Ala Tyr Leu Pro Pro Leu Pro Ser Ile Val Ala Leu Asp Ser 
        115                 120                 125             


Leu Leu Ala Ser Ala Gly Phe Ala Val Pro Ser Ser Ala Leu Asn Gly 
    130                 135                 140                 


Thr Ser Ala Ala Ala Ala Ala Ala Asn Ser Ser Ala Tyr Asn Gly Leu 
145                 150                 155                 160 


Leu Ala Leu Phe Gly Val Thr Gly Asp Met Ala Ser Ala Gly Met Asp 
                165                 170                 175     


Gly Leu Thr Ser Arg Thr Val Thr Ala Ala Gln Gln Leu Arg Ala Phe 
            180                 185                 190         


Leu Ala Gln Leu Pro Ala Leu Ser Ile Pro Gly Gly Pro Ala Ala Thr 
        195                 200                 205             


Gly Gly Gly Pro Ala Gly Ala Gly Ala Gly Ala Gly Ala Gly Ala Gly 
    210                 215                 220                 


Ala Glu Val Asn Ala Thr Ala Ala Ala Asp Glu Ala Ala Gly Leu Arg 
225                 230                 235                 240 


Pro Gly Gln Leu Met Ala Arg Arg Gly Tyr Arg Ala Lys His Pro Val 
                245                 250                 255     


Val Ile Val Pro Gly Phe Val Thr Ser Gly Leu Glu Leu Trp Arg Gly 
            260                 265                 270         


Leu Pro Cys Gly Gln Arg Tyr Phe Arg Gln Arg Met Trp Gly Thr Leu 
        275                 280                 285             


Ala Met Val Gln Ala Phe Leu Thr Asp Ala Ala Cys Trp Phe Arg His 
    290                 295                 300                 


Met Glu Leu Asp Thr Val Ser Gly Leu Asp Pro Glu Gly Ile Lys Leu 
305                 310                 315                 320 


Arg Ala Ala Leu Gly Leu Glu Ala Val Asp Tyr Phe Ile Gln Gly Tyr 
                325                 330                 335     


Trp Val Trp Gly Lys Leu Val Glu Ala Leu Ala Asp Val Gly Tyr Asp 
            340                 345                 350         


Ser Asn Ser Leu Val Ser Met Pro Tyr Asp Trp Arg Leu Ala Val Pro 
        355                 360                 365             


Leu Leu Glu Glu Arg Asp Gly Tyr Tyr Thr Arg Leu Arg Arg Thr Ile 
    370                 375                 380                 


Glu Gln Leu Val Glu Leu Thr Gly Glu Arg Val Val Val Thr Ser His 
385                 390                 395                 400 


Ser Tyr Gly Glu Asn Val Phe Arg Ala Phe Met His Trp Val Glu Ala 
                405                 410                 415     


Ala Ala Ala Glu Glu Glu Glu Gly Gly Lys Gln Pro Arg Ser Gly Gly 
            420                 425                 430         


Gly Ser Gly His Ser Gly Gly Gly Trp Val Asp Arg His Ile Ala Ser 
        435                 440                 445             


Thr Ile Asn Ile Ala Gly Thr Ser Leu Gly Val Pro Lys Ser Val Ser 
    450                 455                 460                 


Ala Leu Leu Ser Gly Glu Thr Arg Asp Thr Ala Gln Leu Gly Ala Leu 
465                 470                 475                 480 


Ala Gly Phe Leu Thr Ser Asn Met Val Pro Arg Ala Ala Arg Thr Arg 
                485                 490                 495     


Val Trp Arg Ser Trp Gly Ala Ser Tyr Ala Met Leu Pro Val Gly Gly 
            500                 505                 510         


Pro Gly Val Trp Gly Asn Ala Ser Trp Ala Pro Asp Asp Thr Pro Glu 
        515                 520                 525             


Met Arg Ala Asn Arg Arg Thr Phe Gly Ser Met Val Ser Leu Trp Pro 
    530                 535                 540                 


His Asn Trp Gln Ala Leu Met Ala Ala Ala Pro Gln Gln Gln Arg Glu 
545                 550                 555                 560 


Gln Leu Ser Gln Leu Val Ala Glu Val Ser Gln Asp Val Asp Ala Gly 
                565                 570                 575     


Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Arg Gly 
            580                 585                 590         


Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Glu Asp Ala Val 
        595                 600                 605             


Asn Gly Thr Gly Gly Ala Gly Ser Ala Gly Gly Ala Ala Leu Ala Pro 
    610                 615                 620                 


Ala Glu Ala Ala Ala Val Gly Thr Ala Gly Gly Gly Asp Asn Ala Thr 
625                 630                 635                 640 


Ala Ala Thr Ala Val Asn Ser Thr Thr Ala Ser Asn Ser Thr Gly Ser 
                645                 650                 655     


Ala Gly Thr Gly Ser Ser Gly Ser Gly Gly Ser Ser Ser Ser Lys Ala 
            660                 665                 670         


Ile Ala Ala Ile Ala Asp Thr Ile Lys Lys Leu Val Gly Gly Gly Val 
        675                 680                 685             


Asp Ala Ala Glu Phe Leu Asn Ser Asp His Val Thr Arg Leu Asp Val 
    690                 695                 700                 


Ser Gly Phe Ile Ala Leu Leu Arg Glu Val Gly Gly Pro Leu Val Ser 
705                 710                 715                 720 


Ala Asn Ile Ala Gln Trp Gly Ala Leu Gln Leu Pro Ala Glu Glu Ala 
                725                 730                 735     


Ala Glu Ala Val Arg Lys Arg Ala Ala Ala Ala Ser Asp Arg Ala Ala 
            740                 745                 750         


Pro Ala Ala Ala Gly Ser Pro Ala Ala Gln Ala Val Gly Gly Ala Asp 
        755                 760                 765             


Arg Val Lys Asp Ser Thr Ala Gly Gly Ala Gly Ala Ala Gly Ile Gly 
    770                 775                 780                 


Arg Gly Gly Ser Ser Ala Gly Ser Gly Arg Val Ala Leu Phe Pro Asp 
785                 790                 795                 800 


Ala Thr Arg Thr Pro Leu Pro Ser Ala Pro Lys Thr Thr Met Val Cys 
                805                 810                 815     


Leu Tyr Gly Val Gly Leu Pro Thr Glu Arg Gly Tyr His Tyr Leu Arg 
            820                 825                 830         


Pro Pro Pro Pro Thr Gly Ala Ala Ala Thr Ala Thr Ala Ala Ala Ala 
        835                 840                 845             


Asn Ala Pro Ala Gly Asn Ala Ala Ala Ala Pro Ala Ala Asp Ala Gln 
    850                 855                 860                 


Pro Pro Pro Gln Ala Ala Gly Gly Gly Asp Gly Asp Gly Met Ala Thr 
865                 870                 875                 880 


Thr Asp Gly Thr Ala Glu Ala Asp Ala Glu Arg Gly Val Pro Pro Arg 
                885                 890                 895     


Gly Ala Thr Ala Ser Gly Glu Glu Ala Asn Pro Asp Ala Gly Gly Asp 
            900                 905                 910         


Ala Ser Ser Gly Trp Met Ile Met Lys Asp Val Thr Gln Ala Gln Ser 
        915                 920                 925             


Ala Leu Asp Val Gly Val His Ile Ser Asp Gly Asp Gly Thr Val Pro 
    930                 935                 940                 


Leu Leu Ser Leu Gly Leu Met Cys Arg Gly Gly Trp Arg Glu Ala Gly 
945                 950                 955                 960 


His Leu Asn Pro Gly Ala Met Arg Val Val Thr Arg Glu Tyr Lys His 
                965                 970                 975     


Lys Ala Val Ser Met Leu Gln Asp Ala Arg Gly Gly Pro Ala Ala Ala 
            980                 985                 990         


Ala His Ile Asp Ile Leu Gly Asn  Asp Ala Val Leu Arg  Asp Val Ile 
        995                 1000                 1005             


Thr Val  Val Ala Gly Arg Ala  Asp Glu Leu Ala Asp  Ile Val Val 
    1010                 1015                 1020             


Ser Asp  Ile Asp Arg Ile Ala  Ala Ala Val Asp Trp  Ala Ala Leu 
    1025                 1030                 1035             


Ala Ala  
    1040 


<210>  3
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Designed primer sequence, Primer CrPDAT-IS1F

<400>  3
tacgactggc ggctggcc                                                     18


<210>  4
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Designed primer sequence, Primer CrPDAT-IS2F

<400>  4
atgctgcctg tgggcggg                                                     18


<210>  5
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Designed primer sequence, Primer CrPDAT-IS1R

<400>  5
cggcactgtt ccgtcgcc                                                     18


<210>  6
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Designed primer sequence, Primer CrPDAT-LS1F

<400>  6
ggcgaggatg ctgtcaatgg aact                                              24


<210>  7
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Designed primer sequence, Primer CrPDAT-LS1R

<400>  7
gctgttgagg aattctgcgg catc                                              24


<210>  8
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Designed primer sequence, Primer CrPDAT-LS2F

<400>  8
atgacgaccc caacaaaagg caatagc                                           27


<210>  9
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Designed primer sequence, Primer CrPDAT-TE1F

<400>  9
cccggagcca tgagagt                                                      17


<210>  10
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Designed primer sequence, Primer CrPDAT-TE1R

<400>  10
cccttgcgtc ctgaacat                                                     18


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Designed primer sequence, Primer 18S rRNA-TE1F

<400>  11
acgagacctc agcctgctaa at                                                22


<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Designed primer sequence, Primer 18S rRNA-TE1R

<400>  12
ttatcgcctc atacttccat tgg                                               23


<210>  13
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Design primer sequence, Primer XhoI-ORFN-YFa

<400>  13
gtgtgtctcg agaaaagaat gacgacccca acaaaaggca atagc                       45


<210>  14
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Designed primer sequence, Primer XhoI-TMN-YFa

<400>  14
gtgtgtctcg agaaaagaac gaccccaaca aaaggcaata gcaatgc                     47


<210>  15
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Designed primer sequence, Primer XbaI-terC-YRa

<400>  15
atcatctcta gagcggccag tgcggcccag tccac                                  35


<210>  16
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORFPDATEF

<400>  16
gtgtgtggat ccatgacgac cccaacaaaa ggcaatagc                              39


<210>  17
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDATER

<400>  17
atcatcaagc tttcaggcgg ccagtgcggc ccagtccac                              39


<210>  18
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TMPDATEF

<400>  18
gtgtgtggat ccgtggagga gggcccagtc gctgagcttc tg                          42


<210>  19
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward oligonucleotides for miRNA silencing

<400>  19
ctagttcgac gcttattcaa gcttaatctc gctgatcggc accatggggg tggtggtgat       60

cagcgctatt aaccttgaat aagcgtcgag                                        90


<210>  20
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse oligonucleotides for miRNA silencing

<400>  20
ctagctcgac gcttattcaa ggttaatagc gctgatcacc accaccccca tggtgccgat       60

cagcgagatt aagcttgaat aagcgtcgaa                                        90


