                                SEQUENCE LISTING

<110> Lou, Chunbo
      Moon, Tae Seok
      Rhodius, Virgil
      Stanton, Brynne
      Tamsir, Alvin
      Temme, Karsten
      Voigt, Chris
      The Regents of the University of California

<120> Synthetic Biology Tools
  

<130> 81906-839851

<140> WO Not yet assigned 
<141> Not yet assigned  

<150> US 61/493,733       
<151> 2011-06-06  

<160> 244

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(66)
<223> n = g, a, c or t

<400> 1
nnnntaaann tnnnnaaatn annannntnn tgaaacnttt tnnnnnnnna nnncgtataa 60
naaann                                                            66

<210> 2
<211> 62
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(62)
<223> n = g, a, c or t

<400> 2
nnnaannnan aaaaaattnn annnnnnnnn gaacttttcn aatannnnnn agtctaannn 60
ng                                                                62

<210> 3
<211> 69
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(69)
<223> n = g, a, c or t

<400> 3
antnanaatt ntaatattnt ttanaggtga ttaaaccttt tgtctaaatn atagtctaac 60
accaaanaa                                                         69

<210> 4
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(48)
<223> n = g, a, c or t

<400> 4
nnnnnnnnnn nnnntnngna aaatnnnnnn nnnntnnntt gtntnncn              48

<210> 5
<211> 68
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(68)
<223> n = g, a, c or t

<400> 5
nnnnnnnnnn naataattna tannntnnag tgatccaatt tcnancccgc cacgtattaa 60
ntnanana                                                          68

<210> 6
<211> 67
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(67)
<223> n = g, a, c or t

<400> 6
nncggnaanc cnnncagcgg acncgcggcc gggaataaca cggncgccnn cnctgttgnn 60
nggancg                                                           67

<210> 7
<211> 69
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(69)
<223> n = g, a, c or t

<400> 7
tttcccnggt agcgaaangn acagccnggt ttctcaggna aagctcagat tgctcatatc 60
ccgcccata                                                         69

<210> 8
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(66)
<223> n = g, a, c or t

<400> 8
nnnnnnncna caaaannncn cccnncnnnn ggaacnntnn cnncnacnnn gcgttncgnn 60
cnnnnn                                                            66

<210> 9
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(70)
<223> n = g, a, c or t

<400> 9
agnngcngnt annnaacntt tnggnggcga tgtaaccaan nncgnccnac ncgcgaataa 60
gnnggnaggg                                                        70

<210> 10
<211> 67
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(67)
<223> n = g, a, c or t

<400> 10
gggcnccgcg gcnncncgcn ggncgcgggc tgaaccgaac ngcngccggc gtcgtgtnnn 60
tggcggg                                                           67

<210> 11
<211> 68
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(68)
<223> n = g, a, c or t

<400> 11
cggcnnnngc nncagnnnnn gcgcggnntc tgcatccacn nnnncccgnt ncccgtatnn 60
nnanncnn                                                          68

<210> 12
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(54)
<223> n = g, a, c or t

<400> 12
gnnnncnnnn ncngncccac cgatccnnnn cancnncnnc tccgaatann nnng       54

<210> 13
<211> 68
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(68)
<223> n = g, a, c or t

<400> 13
ncantnncnn naaaannnnn nnattngncc gacggattgn nnnnnnngnn ncgttcaann 60
nncngann                                                          68

<210> 14
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(70)
<223> n = g, a, c or t

<400> 14
antanannan ngnntttttt caanacnnca tgcaaccntc ntnnanannn ttgcgtctat 60
nnnaatagaa                                                        70

<210> 15
<211> 69
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(69)
<223> n = g, a, c or t

<400> 15
nnnnanaant tcnaattatt ttagttannn tgtaataatt tncnnntttn nacgactaat 60
nnataaaaa                                                         69

<210> 16
<211> 68
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(68)
<223> n = g, a, c or t

<400> 16
tnatttnatg cntncnanaa atngacaatg tctggaanaa taccaanaga taaaatcatt 60
agatanta                                                          68

<210> 17
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(66)
<223> n = g, a, c or t

<400> 17
tganngagtc tanaatnnga cacnaantcc aggaacaant gtngnaccag nacgtcaaat 60
aaanag                                                            66

<210> 18
<211> 68
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(68)
<223> n = g, a, c or t

<400> 18
nnnncnntnn ngnnantttc gncnnnggcg ggaataaact ccgcccnncn ggcgtttnnn 60
cnnnnann                                                          68

<210> 19
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(52)
<223> n = g, a, c or t

<400> 19
nccggnngcn ccnccncnnc gggaacancn nnccctaagc tggngttgnn nn         52

<210> 20
<211> 71
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(71)
<223> n = g, a, c or t

<400> 20
cnncnancng cgcangcann ngnnacnagc cgggaacgcn ngcnccnccc ncgccatcca 60
atgcacggag g                                                      71

<210> 21
<211> 71
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(71)
<223> n = g, a, c or t

<400> 21
tnataaatat ttnaattaat ttanaaattt atgaaacttt ttttaannnt natncgtcta 60
ataaatgtaa a                                                      71

<210> 22
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(70)
<223> n = g, a, c or t

<400> 22
nnnntgtntt tttannnant tnanaaannn ntgaactttt tgntnttnng nggcgtctna 60
tatanagaaa                                                        70

<210> 23
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(53)
<223> n = g, a, c or t

<400> 23
tnnnnnnncn cctngtnngg gaaccgatcc gtnnntnnnn ccacacagcg ana        53

<210> 24
<211> 70
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(70)
<223> n = g, a, c or t

<400> 24
ccnnnngttt gtancatttt gcnctgccng acgaaacatn tccncnnncc ncgaaaccaa 60
tttcccgaga                                                        70

<210> 25
<211> 71
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(71)
<223> n = g, a, c or t

<400> 25
ttgnaacnna nctttatgtt gcgaaccgnn gacgatagcg ggtngcccnn gngcggtcaa 60
gcnaanaatc c                                                      71

<210> 26
<211> 71
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(71)
<223> n = g, a, c or t

<400> 26
gnncnccccc gcgaccnggt ngnccnggng gtacacccnn ggcggggngg cncgcgtcca 60
actggcgtga c                                                      71

<210> 27
<211> 68
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(68)
<223> n = g, a, c or t

<400> 27
cgggcgcncc ngncccgtnc ngcccncccg tcaaccgnnc ccgccgcgcc ngcgtccggn 60
gggngncg                                                          68

<210> 28
<211> 69
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(69)
<223> n = g, a, c or t

<400> 28
nnnnnncngg cnnnnnnnnn cncgcgtcga tgtcacannc gccgggnctg nctcgtcttg 60
tnngcgnaa                                                         69

<210> 29
<211> 67
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(67)
<223> n = g, a, c or t

<400> 29
nngnnncnnc acaaatnntt tngcccggga tgtcgatnnn gcgcnnnccc gttcgtcccn 60
nggntga                                                           67

<210> 30
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(50)
<223> n = g, a, c or t

<400> 30
naaaaatnca tnannggntg aacttttnca anannncnna gtctaaannn            50

<210> 31
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic promoter sequence motif

<220> 
<221> modified_base  
<222> (1)...(50)
<223> n = g, a, c or t

<400> 31
nnantancnn nnnntccngt gatccaaacn nncnncagcn ncgtatnana            50

<210> 32
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic parental promoter sequence

<400> 32
tgcgtaattt attcacaagc ttgcatggaa cttgtggata aaatcacggt ctgataaaac 60
agtgaatgat aacctcgttg                                             80

<210> 33
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic parental promoter sequence

<400> 33
gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg taacacctct 60
gcatcgcgaa ccaaaaccag                                             80

<210> 34
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic chimeric promoter sequence

<400> 34
tgcgtaattt attcacaagc ttgcatggaa cttgtggata tcccgctacg taacacctct 60
gcatcgcgaa ccaaaaccag                                             80

<210> 35
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic chimeric promoter sequence

<400> 35
gcctccacac cgctcgtcac atcctgtgat ccactctata aaatcacggt ctgataaaac 60
agtgaatgat aacctcgttg                                             80

<210> 36
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic J23119 promoter and operator sequence insert

<400> 36
ttgacagcta gctcagtcct aggtataatc gtagc                            35

<210> 37
<211> 67
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic inverted repeat sequence

<220> 
<221> repeat_unit    
<222> (3)...(16)
<223> 14-mer repeat unit, N-14 through N-1

<220> 
<221> repeat_unit    
<222> (17)...(30)
<223> 14-mer repeat unit, N-1 through N-14

<220> 
<221> repeat_unit    
<222> (38)...(51)
<223> 14-mer repeat unit, N-14 through N-1

<220> 
<221> repeat_unit    
<222> (52)...(65)
<223> 14-mer repeat unit, N-1 through N-14

<220> 
<221> modified_base  
<222> (1)...(67)
<223> n = g, a, c or t

<400> 37
gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgaggcnnn nnnnnnnnnn nnnnnnnnnn 60
nnnnngc                                                           67

<210> 38
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic repressor consensus sequence, ButR
      consensus sequence

<400> 38
gtgtcactca aa                                                     12

<210> 39
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 RNAP N77 scaffold

<400> 39
aattgtgagc ggataacaa                                              19

<210> 40
<211> 120
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 RNAP N115 scaffold

<400> 40
atgttgttta tcaagcctgc ggatctccgc gaaattgtga cttttccgct atttagcgat 60
cttgttcagt gtggctttcc ttcaccggca gcagattacg ttgaacagcg catcgatctg 120


<210> 41
<211> 75
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 RNAP N121 scaffold

<400> 41
atgttgttta tcaagcctgc ggatctccgc gaaattgtga cttttccgct atttagcgat 60
cttgttcagt gtggc                                                  75

<210> 42
<211> 120
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 RNAP N130 scaffold

<400> 42
atgttgttta tcaagcctgc ggatctccgc gaaattgtga ctgccgcggc agcgagcgat 60
cttgttcagt gtggctttcc ttcaccggca gcagattacg ttgaacagcg catcgatctg 120


<210> 43
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 RNAP N249 scaffold

<400> 43
tatccaaacc agtagctcaa ttggagtcgt ctat                             34

<210> 44
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 RNAP N249 scaffold random DNA spacer

<400> 44
tgcagtttta ttctctcgcc agcactgtaa taggcactaa                       40

<210> 45
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 promoter sequence motif

<220> 
<221> modified_base  
<222> (16)...(20)
<223> n = g, a, c or t

<400> 45
taatacgact cactannnnn aga                                         23

<210> 46
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 wild-type (WT) promoter sequence

<400> 46
taatacgact cactataggg aga                                         23

<210> 47
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 Mut1 mutant promoter sequence

<400> 47
taatacgact cactacaggc aga                                         23

<210> 48
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 Mut2 mutant promoter sequence

<400> 48
taatacgact cactagagag aga                                         23

<210> 49
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 Mut3 mutant promoter sequence

<400> 49
taatacgact cactaatggg aga                                         23

<210> 50
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 Mut4 mutant promoter sequence

<400> 50
taatacgact cactataggt aga                                         23

<210> 51
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 Mut5 mutant promoter sequence

<400> 51
taatacgact cactaaaggg aga                                         23

<210> 52
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7 Mut6 mutant promoter sequence

<400> 52
taatacgact cactattggg aga                                         23

<210> 53
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic transcriptional terminator sequence motif

<220> 
<221> modified_base  
<222> (3)...(6)
<223> n = g, a, c or t

<400> 53
tannnnaacc sswwssssss tcwwwwcgss sssswwssgg ttttttgt              48

<210> 54
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant transcriptional terminator 52
      sequence

<400> 54
tataaaacgg ggggctaggg gttttttgt                                   29

<210> 55
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant transcriptional terminator 23
      sequence

<400> 55
tactcgaacc cctagcccgc tcttatcggg cggctagggg ttttttgt              48

<210> 56
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant transcriptional terminator 72
      sequence

<400> 56
tagcagaacc gctaacgggg gcgaaggggt tttttgt                          37

<210> 57
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant transcriptional terminator 48
      sequence

<400> 57
tactcgaacc cctagcccgc tcttatcggg cggctagggg ttttttgt              48

<210> 58
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant transcriptional terminator 1
      sequence

<400> 58
tacatatcgg gggggtaggg gttttttgt                                   29

<210> 59
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant transcriptional terminator 2
      sequence

<400> 59
tacatatcgg gggggtaggg gttttttgt                                   29

<210> 60
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic wild-type (WT) transcriptiona terminator
      sequence

<400> 60
tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgt              48

<210> 61
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant transcriptional terminator 31
      sequence

<400> 61
taccctaacc ccttccccgg tcaatcgggg cggatggggt tttttgt               47

<210> 62
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant transcriptional terminator 58
      sequence

<400> 62
tagaccaacc ccttgcggcc tcaatcgggg gggatggggt tttttgt               47

<210> 63
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant transcriptional terminator 25
      sequence

<400> 63
tactctaacc ccatcggccg tcttaggggt tttttgt                          37

<210> 64
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutant transcriptional terminator 17
      sequence

<400> 64
tacctcaacc ccttccgccc tcatatcgcg gggcatgcgg ttttttgt              48

<210> 65
<211> 39
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic T7 RNAP specificity loop sequence

<400> 65
Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met
 1               5                  10                  15      
Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp
            20                  25                  30          
Ser Glu Ile Asp Ala His Lys
        35                  


<210> 66
<211> 39
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic T3 RNAP specificity loop sequence

<400> 66
Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Lys Arg Leu Asp Met Ile
 1               5                  10                  15      
Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp
            20                  25                  30          
Ser Glu Ile Asp Ala His Lys
        35                  


<210> 67
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T3 promoter sequence

<400> 67
taataaccct cactataggg aga                                         23

<210> 68
<211> 39
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic K1F RNAP specificity loop sequence

<400> 68
Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met
 1               5                  10                  15      
Phe Leu Gly Ser Phe Asn Leu Gln Pro Thr Val Asn Thr Asn Lys Asp
            20                  25                  30          
Ser Glu Ile Asp Ala His Lys
        35                  


<210> 69
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic K1F promoter sequence

<400> 69
taataactat cactataggg aga                                         23

<210> 70
<211> 39
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic N4 RNAP specificity loop sequence

<400> 70
Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Ile Asp Cys Val
 1               5                  10                  15      
Ile Leu Gly Thr His Arg Met Ala Leu Thr Ile Asn Thr Asn Lys Asp
            20                  25                  30          
Ser Glu Ile Asp Ala His Lys
        35                  


<210> 71
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic N4 promoter sequence

<400> 71
taataaccca cactataggg aga                                         23

<210> 72
<211> 15
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic partial T7 promoter sequence

<400> 72
taatacgact cacta                                                  15

<210> 73
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7:Mut1 promoter sequence

<400> 73
taatacgact cactacaggc aga                                         23

<210> 74
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7:Mut2 promoter sequence

<400> 74
taatacgact cactagagag aga                                         23

<210> 75
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7:Mut3 promoter sequence

<400> 75
taatacgact cactaatggg aga                                         23

<210> 76
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7:Mut4 promoter sequence

<400> 76
taatacgact cactataggt aga                                         23

<210> 77
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7:Mut5 promoter sequence

<400> 77
taatacgact cactaaaggg aga                                         23

<210> 78
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T7:Mut6 promoter sequence

<400> 78
taatacgact cactattggg aga                                         23

<210> 79
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T3:Mut1 promoter sequence

<400> 79
taataaccct cactacaggc aga                                         23

<210> 80
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T3:Mut2 promoter sequence

<400> 80
taataaccct cactagagag aga                                         23

<210> 81
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T3:Mut3 promoter sequence

<400> 81
taataaccct cactaatggg aga                                         23

<210> 82
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T3:Mut4 promoter sequence

<400> 82
taataaccct cactataggt aga                                         23

<210> 83
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T3:Mut5 promoter sequence

<400> 83
taataaccct cactaaaggg aga                                         23

<210> 84
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic T3:Mut6 promoter sequence

<400> 84
taataaccct cactattggg aga                                         23

<210> 85
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic AmeR repressor consensus sequence

<220> 
<221> modified_base  
<222> (9)...(9)
<223> n = g, a, c or t

<400> 85
tagtgacgnt ta                                                     12

<210> 86
<211> 14
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic JadR2 repressor consensus sequence

<220> 
<221> modified_base  
<222> (7)...(8)
<223> n = g, a, c or t

<400> 86
agatacnngt atct                                                   14

<210> 87
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic ArpA repressor consensus sequence

<400> 87
tccacatgta gc                                                     12

<210> 88
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic CasR repressor consensus sequence

<400> 88
tgagtactgt aa                                                     12

<210> 89
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic LitR repressor consensus sequence

<400> 89
gcttatatgc                                                        10

<210> 90
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic BarA repressor consensus sequence

<400> 90
gcacgatcat gatcgtgc                                               18

<210> 91
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic DhaR repressor consensus sequence

<400> 91
acggcatgta tc                                                     12

<210> 92
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic McbR repressor consensus sequence

<220> 
<221> modified_base  
<222> (2)...(2)
<223> n = g, a, c or t

<400> 92
tntagacaga cc                                                     12

<210> 93
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic BarB repressor consensus sequence

<220> 
<221> modified_base  
<222> (2)...(10)
<223> n = g, a, c or t

<400> 93
gcngaataan at                                                     12

<210> 94
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic EnvR repressor consensus sequence

<400> 94
ggccaatgag ta                                                     12

<210> 95
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic repressor consensus sequence

<400> 95
agactagaca ga                                                     12

<210> 96
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic degenerate ribosomal binding site (RBS) library

<400> 96
tcacacggaa akrcywsg                                               18

<210> 97
<211> 206
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic chimeric sigma factor ECF11_ECF02

<400> 97
Met Arg Ile Thr Ala Ser Leu Arg Thr Phe Cys His Leu Ser Thr Pro
 1               5                  10                  15      
His Ser Asp Ser Thr Thr Ser Arg Leu Trp Ile Asp Glu Val Thr Ala
            20                  25                  30          
Val Ala Arg Gln Arg Asp Arg Asp Ser Phe Met Arg Ile Tyr Asp His
        35                  40                  45              
Phe Ala Pro Arg Leu Leu Arg Tyr Leu Thr Gly Leu Asn Val Pro Glu
    50                  55                  60                  
Gly Gln Ala Glu Glu Leu Val Gln Glu Val Leu Leu Lys Leu Trp His
65                  70                  75                  80  
Lys Ala Glu Ser Phe Asp Pro Ser Lys Ala Ser Leu Gly Thr Trp Leu
                85                  90                  95      
Phe Arg Ile Ala Arg Asn Leu Tyr Ile Asp Ser Val Arg Lys Asp Arg
            100                 105                 110         
Gly Trp Val Gln Val Gln Asn Ser Leu Glu Gln Leu Glu Arg Leu Glu
        115                 120                 125             
Ala Ile Ser Asn Pro Glu Asn Leu Met Leu Ser Glu Glu Leu Arg Gln
    130                 135                 140                 
Ile Val Phe Arg Thr Ile Glu Ser Leu Pro Glu Asp Leu Arg Met Ala
145                 150                 155                 160 
Ile Thr Leu Arg Glu Leu Asp Gly Leu Ser Tyr Glu Glu Ile Ala Ala
                165                 170                 175     
Ile Met Asp Cys Pro Val Gly Thr Val Arg Ser Arg Ile Phe Arg Ala
            180                 185                 190         
Arg Glu Ala Ile Asp Asn Lys Val Gln Pro Leu Ile Arg Arg
        195                 200                 205     


<210> 98
<211> 187
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic chimeric sigma factor ECF02_ECF11

<400> 98
Met Ser Glu Gln Leu Thr Asp Gln Val Leu Val Glu Arg Val Gln Lys
 1               5                  10                  15      
Gly Asp Gln Lys Ala Phe Asn Leu Leu Val Val Arg Tyr Gln His Lys
            20                  25                  30          
Val Ala Ser Leu Val Ser Arg Tyr Val Pro Ser Gly Asp Val Pro Asp
        35                  40                  45              
Val Val Gln Glu Ala Phe Ile Lys Ala Tyr Arg Ala Leu Asp Ser Phe
    50                  55                  60                  
Arg Gly Asp Ser Ala Phe Tyr Thr Trp Leu Tyr Arg Ile Ala Val Asn
65                  70                  75                  80  
Thr Ala Lys Asn Tyr Leu Val Ala Gln Gly Arg Arg Pro Pro Ser Ser
                85                  90                  95      
Asp Val Asp Ala Ile Glu Ala Glu Asn Phe Glu Gln Leu Glu Arg Leu
            100                 105                 110         
Glu Ala Pro Val Asp Arg Thr Leu Asp Tyr Ser Gln Arg Gln Glu Gln
        115                 120                 125             
Gln Leu Asn Ser Ala Ile Gln Asn Leu Pro Thr Asp Gln Ala Lys Val
    130                 135                 140                 
Leu Arg Met Ser Tyr Phe Glu Ala Leu Ser His Arg Glu Ile Ser Glu
145                 150                 155                 160 
Arg Leu Asp Met Pro Leu Gly Thr Val Lys Ser Cys Leu Arg Leu Ala
                165                 170                 175     
Phe Gln Lys Leu Arg Ser Arg Ile Glu Glu Ser
            180                 185         


<210> 99
<211> 883
<212> PRT
<213> Enterobacteria phage T7

<220> 
<223> bacteriophage T7 RNA polymerase (RNAP)

<400> 99
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu
 1               5                  10                  15      
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu
            20                  25                  30          
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu
        35                  40                  45              
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val
    50                  55                  60                  
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys
65                  70                  75                  80  
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg
                85                  90                  95      
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu
            100                 105                 110         
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser
        115                 120                 125             
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala
    130                 135                 140                 
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys
145                 150                 155                 160 
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His
                165                 170                 175     
Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser
            180                 185                 190         
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp
        195                 200                 205             
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr
    210                 215                 220                 
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp
225                 230                 235                 240 
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr
                245                 250                 255     
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val
            260                 265                 270         
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala
        275                 280                 285             
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala
    290                 295                 300                 
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile
305                 310                 315                 320 
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala
                325                 330                 335     
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile
            340                 345                 350         
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp
        355                 360                 365             
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val
    370                 375                 380                 
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe
385                 390                 395                 400 
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe
                405                 410                 415     
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe
            420                 425                 430         
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys
        435                 440                 445             
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly
    450                 455                 460                 
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys
465                 470                 475                 480 
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro
                485                 490                 495     
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu
            500                 505                 510         
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr
        515                 520                 525             
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln
    530                 535                 540                 
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn
545                 550                 555                 560 
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys
                565                 570                 575     
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn
            580                 585                 590         
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys
        595                 600                 605             
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly
    610                 615                 620                 
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly
625                 630                 635                 640 
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln
                645                 650                 655     
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln
            660                 665                 670         
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr
        675                 680                 685             
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys
    690                 695                 700                 
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg
705                 710                 715                 720 
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp
                725                 730                 735     
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu
            740                 745                 750         
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu
        755                 760                 765             
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His
    770                 775                 780                 
Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu
785                 790                 795                 800 
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr
                805                 810                 815     
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met
            820                 825                 830         
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln
        835                 840                 845             
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu
    850                 855                 860                 
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe
865                 870                 875                 880 
Ala Phe Ala
            

<210> 100
<211> 498
<212> DNA
<213> Salmonella typhimurium

<220> 
<223> transcriptional activator chaperone protein sicA

<400> 100
atggattatc aaaataatgt cagcgaagaa cgtgttgcgg aaatgatttg ggatgccgtt 60
agtgaaggcg ccacgctaaa agacgttcat gggatccctc aagatatgat ggacggttta 120
tatgctcatg cttatgagtt ttataaccag ggacgactgg atgaagctga gacgttcttt 180
cgtttcttat gcatttatga tttttacaat cccgattaca ccatgggact ggcggcagta 240
tgccaactga aaaaacaatt tcagaaagca tgtgaccttt atgcagtagc gtttacgtta 300
cttaaaaatg attatcgccc cgtttttttt accgggcagt gtcaattatt aatgcgtaag 360
gcagcaaaag ccagacagtg ttttgaactt gtcaatgaac gtactgaaga tgagtctctg 420
cgggcaaaag cgttggtcta tctggaggcg ctaaaaacgg cggagacaga gcagcacagt 480
gaacaagaaa aggaataa                                               498

<210> 101
<211> 498
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic sicA* F62Y mutant sicA transcriptional
      activator chaperone protein

<400> 101
atggattatc aaaataatgt cagcgaagaa cgtgttgcgg aaatgatttg ggatgccgtt 60
agtgaaggcg ccacgctaaa agacgttcat gggatccctc aagatatgat ggacggttta 120
tatgctcatg cttatgagtt ttataaccag ggacgactgg atgaagctga gacgttcttt 180
cgttacttat gcatttatga tttttacaat cccgattaca ccatgggact ggcggcagta 240
tgccaactga aaaaacaatt tcagaaagca tgtgaccttt atgcagtagc gtttacgtta 300
cttaaaaatg attatcgccc cgtttttttt accgggcagt gtcaattatt aatgcgtaag 360
gcagcaaaag ccagacagtg ttttgaactt gtcaatgaac gtactgaaga tgagtctctg 420
cgggcaaaag cgttggtcta tctggaggcg ctaaaaacgg cggagacaga gcagcacagt 480
gaacaagaaa aggaataa                                               498

<210> 102
<211> 750
<212> DNA
<213> Salmonella typhimurium

<220> 
<223> transcriptional activator invF with new corrected
      upstream start codon

<400> 102
atgctaaata cgcaggaagt acttaaagaa ggagagaagc ggaaaatccg cagcccggaa 60
gcatggttta tacagacgtg ttccgcgcaa aagctgcata tgtcattttc tgaaagccga 120
cacaatgaaa attgcctgat tcaggaaggc gcgctgcttt tttgcgagca ggccgttgtc 180
gcaccagtat caggagacct ggtttttcga ccgttaaaaa ttgaagtact cagcaaatta 240
ctggcattta tcgatggcgc aggattagtg gacacgacat atgctgaatc cgataaatgg 300
gttttgctga gtcctgagtt tcgcgctatt tggcaagatc gtaaacgctg cgagtactgg 360
tttttgcagc aaattattac gccttctccg gccttcaata aggtactggc gctgttacga 420
aaaagcgaga gttactggtt ggttggctat ttactcgctc agtcaaccag cggcaacacg 480
atgagaatgc tgggagaaga ctatggcgtt tcttataccc attttcgtcg tttgtgcagc 540
agagcgttgg gcggaaaagc gaagagtgaa ttacgaaact ggcgtatggc gcaatcgctg 600
ctgaatagtg tagaaggcca cgagaacatc acccaattag ccgttaatca tggttactca 660
tcgccttcac atttttctag tgagatcaaa gagctgatcg gcgtttcgcc gcggaaatta 720
tcaaatatta ttcaattggc agacaaatga                                  750

<210> 103
<211> 163
<212> DNA
<213> Salmonella typhimurium

<220> 
<221> promoter       
<222> (1)...(163)
<223> psicA promoter

<400> 103
ccacaagaaa cgaggtacgg cattgagccg cgtaaggcag tagcgatgta ttcattgggc 60
gttttttgaa tgttcactaa ccaccgtcgg ggtttaataa ctgcatcaga taaacgcagt 120
cgttaagttc tacaaagtcg gtgacagata acaggagtaa gta                   163

<210> 104
<211> 468
<212> DNA
<213> Shigella flexneri

<220> 
<223> transcriptional activator chaperone protein ipgC

<400> 104
atgtctttaa atatcaccga aaatgaaagc atctctactg cagtaattga tgcaattaac 60
tctggcgcta cactgaaaga tattaatgca attcctgatg atatgatgga tgacatttat 120
tcatatgctt atgactttta caacaaagga agaatagagg aagctgaagt tttcttcagg 180
tttttatgta tatacgactt ttacaatgta gactacatta tgggactcgc agctatttat 240
cagataaaag aacagttcca acaagcagca gacctttatg ctgtcgcttt tgcattagga 300
aaaaatgact atacaccagt attccatact ggacaatgtc agcttcggtt gaaagccccc 360
ttaaaagcta aagagtgctt cgaactcgta attcaacaca gcaatgatga aaaattaaaa 420
ataaaagcac aatcatactt ggacgcaatt caggatatca aggagtaa              468

<210> 105
<211> 756
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic Shigella flexneri transcriptional
      activator mxiE with codon optimization

<400> 105
atgagtaaat ataaaggcct gaacaccagc aacatgttct acatctacag ctctggtcat 60
gaaccggtga acgttgaact ggtgaaagat aaagaacgta acatcatcga actggcaccg 120
gcgtggaaag gctttttctt tgtgcgtaac cagaacatca aattcagcga taacgttaac 180
taccactacc gcttcaacat caactcttgc gcaaaattcc tggcgttttg ggattatttt 240
agcggcgccc tggttgaaca ttctcacgca gaaaaatgca tccatttcta ccacgaaaac 300
gatctgcgtg atagctgtaa tacggaatct atgctggata aactgatgct gcgcttcatt 360
tttagtagcg atcagaacgt gtctaatgcc ctggcaatga tccgtatgac cgaaagttat 420
catctggttc tgtacctgct gcgtacgatt gaaaaagaaa aagaagtgcg catcaaaagc 480
ctgaccgaac actatggcgt ttctgaagcg tactttcgta gtctgtgtcg caaagcgctg 540
ggtgccaaag tgaaagaaca gctgaacacg tggcgcctgg tgaatggcct gctggatgtt 600
ttcctgcata accagaccat tacgagcgcg gccatgaaca atggttatgc gtctaccagt 660
cacttcagca atgaaattaa aacgcgtctg ggctttagtg cccgcgaact gagcaacatc 720
accttcctgg tgaagaaaat taatgaaaaa atctaa                           756

<210> 106
<211> 350
<212> DNA
<213> Shigella flexneri

<220> 
<221> promoter       
<222> (1)...(350)
<223> pipaH9.8 promoter

<400> 106
gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct 60
agggtcaaaa atcgtggcgt tgacaaaatg gctgcgttac gtcattgagc atatccagga 120
ctggccggca aaccgggtac gcgatctgtt gccttggaaa gttgatctga cctctcagta 180
aatatcaata cggttctgac gagccgctta ccgttcaaat atgaagtacg atgtttaact 240
aaccgaaaaa caagaacaat acggtgcaaa caggccattc acggttaact gaaacagtat 300
cgttttttta cagccaattt tgtttatcct tattataata aaaaagtgct            350

<210> 107
<211> 350
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic mutated pipaH9.8 Shigella flexneri
      promoter pipaH9.8*

<400> 107
gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct 60
agggtcaaaa atcgtggcgt tgacaaaatg gctgcgttac gtcattgagc atatccagga 120
ctggccggca aaccgggtac gcgatctgtt gccttggaaa gttgatctga cctctcagta 180
aatatcaata cggttctgac gagccgctta ccgttcaaat atgaagtacg atgtttaact 240
aaccgaaaaa caagaacaat acggtgcaaa caggccattc acggttaact gaaacagtat 300
cgttttttta cagccaattt tgtttatcct tattaagata aaaaagtgct            350

<210> 108
<211> 438
<212> DNA
<213> Pseudomonas aeruginosa

<220> 
<223> transcriptional activator chaperone protein exsC

<400> 108
atggatttaa cgagcaaggt caaccgactg cttgccgagt tcgcaggccg tatcggtttg 60
ccttccctgt ccctcgacga ggagggcatg gcgagcctcc tgttcgacga acaggtgggc 120
gtcaccctgt tgctgctcgc cgagcgcgag cgtctgttgc tggaggccga tgtggcgggc 180
atcgatgtgc tgggcgaggg gatctttcgc cagctcgcca gcttcaaccg ccattggcac 240
cgtttcgatc tgcatttcgg cttcgacgag ctgaccggca aggtccagtt gtatgcgcag 300
attctcgcag cgcaactgac cctcgaatgc ttcgaggcga ccttggccaa tctgctcgat 360
cacgccgagt tctggcagcg cctgctgccg tgcgacagtg atcgcgaggc ggtcgctgcg 420
gtcggcatga gggtttga                                               438

<210> 109
<211> 831
<212> DNA
<213> Pseudomonas aeruginosa

<220> 
<223> exsD

<400> 109
atggagcagg aagacgataa gcagtactcc cgagaagcgg tgttcgctgg caggcgggta 60
tccgtggtgg gctcggacgc ccgctcgcgg ggtcgggtgc cgggttacgc atcgagcagt 120
ttgtatcgtg agtccggaat catcagtgcg cggcaactgg cgttgctgca gcggatgctg 180
ccgcgcctgc ggctggagca actgttccgc tgcgagtggt tgcagcagcg cctggcgcgc 240
ggcctggcgc tggggcgcga agaggtgcgg cagattctcc tctgcgcggc gcaggacgac 300
gacggctggt gctccgaact gggcgaccgg gtcaacctcg ccgtgccgca gtcgatgatc 360
gactgggtcc tgctgccggt ctatggctgg tgggaaagcc tgctcgacca ggcgatcccc 420
ggctggcgcc tgtcgctggt ggagctggag acccagtccc ggcaactgcg agtcaagtcc 480
gaattctggt cccgcgtggc cgagctggag ccggagcagg cccgcgagga actggccagg 540
gtcgccaagt gccaggcgcg cacccaggaa caggtggccg aactggccgg caagctggag 600
acggcttcgg cactggcgaa gagcgcctgg ccgaactggc agcggggcat ggcgacgctg 660
ctcgccagcg gcgggctggc cggcttcgag ccgatccccg aggtcctcga atgcctctgg 720
caacctctct gccggctgga cgacgacgtc ggcgcggcgg acgccgtcca ggcctggctg 780
cacgaacgca acctgtgcca ggcacaggat cacttctact ggcagagctg a          831

<210> 110
<211> 837
<212> DNA
<213> Pseudomonas aeruginosa

<220> 
<223> transcriptional activator exsA

<400> 110
atgcaaggag ccaaatctct tggccgaaag cagataacgt cttgtcattg gaacattcca 60
actttcgaat acagggtaaa caaggaagag ggcgtatatg ttctgctcga gggcgaactg 120
accgtccagg acatcgattc cactttttgc ctggcgcctg gcgagttgct tttcgtccgc 180
cgcggaagct atgtcgtaag taccaaggga aaggacagcc gaatactctg gattccatta 240
tctgcccagt ttctacaagg cttcgtccag cgcttcggcg cgctgttgag tgaagtcgag 300
cgttgcgacg agcccgtgcc gggcatcatc gcgttcgctg ccacgcctct gctggccggt 360
tgcgtcaagg ggttgaagga attgcttgtg catgagcatc cgccgatgct cgcctgcctg 420
aagatcgagg agttgctgat gctcttcgcg ttcagtccgc aggggccgct gctgatgtcg 480
gtcctgcggc aactgagcaa ccggcatgtc gagcgtctgc agctattcat ggagaagcac 540
tacctcaacg agtggaagct gtccgacttc tcccgcgagt tcggcatggg gctgaccacc 600
ttcaaggagc tgttcggcag tgtctatggg gtttcgccgc gcgcctggat cagcgagcgg 660
agaatcctct atgcccatca gttgctgctc aacagcgaca tgagcatcgt cgacatcgcc 720
atggaggcgg gcttttccag tcagtcctat ttcacccaga gctatcgccg ccgtttcggc 780
tgcacgccga gccgctcgcg gcaggggaag gacgaatgcc gggctaaaaa taactga    837

<210> 111
<211> 80
<212> DNA
<213> Pseudomonas aeruginosa

<220> 
<221> promoter       
<222> (1)...(80)
<223> pexsD promoter

<400> 111
gaaggacgaa tgccgggcta aaaataactg acgttttttg aaagcccggt agcggctgca 60
tgagtagaat cggcccaaat                                             80

<210> 112
<211> 80
<212> DNA
<213> Pseudomonas aeruginosa

<220> 
<221> promoter       
<222> (1)...(80)
<223> pexsC promoter

<400> 112
gatgtggctt ttttcttaaa agaaaagtct ctcagtgaca aaagcgatgc atagcccggt 60
gctagcatgc gctgagcttt                                             80

<210> 113
<211> 702
<212> DNA
<213> Discosoma sp.

<220> 
<223> red fluorescent protein (RFP) reporter protein

<400> 113
atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt 60
tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt 120
acgcagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc 180
ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg 240
gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa 300
gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac 360
aaagttaaac tgcgtggtac taacttcccg tccgacggtc cggttatgca gaaaaaaacc 420
atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa 480
atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc 540
tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac 600
atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt 660
cactccaccg gtgctgcagc aaacgacgaa aactacgctt aa                    702

<210> 114
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for McbR repressor

<400> 114
tgaacagctt ggtcta                                                 16

<210> 115
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for UidR repressor

<400> 115
ctattggtta accaattt                                               18

<210> 116
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for BM3R1 repressor

<400> 116
cggaatgaac gttcattccg                                             20

<210> 117
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for AmtR repressor

<400> 117
attatctata gatcgataga aa                                          22

<210> 118
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for BetI repressor

<400> 118
ttatattgaa cgtccaatga at                                          22

<210> 119
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for HapR repressor

<400> 119
ttattgattt ttaatcaaat aa                                          22

<210> 120
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for HlyIIR repressor

<400> 120
tttaaacaag aattttaaat at                                          22

<210> 121
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for SmcR repressor

<400> 121
ttattgataa atctgcgtaa aa                                          22

<210> 122
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for AcrR repressor

<400> 122
tacatacatt tatgaatgta tgta                                        24

<210> 123
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for ArpA repressor

<400> 123
cgacatacgg gacgccccgt ttat                                        24

<210> 124
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for LmrA repressor

<400> 124
agataataga ccagtcacta tatt                                        24

<210> 125
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for BarA repressor

<400> 125
agatacatac caaccggttc ttttga                                      26

<210> 126
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for QacR repressor

<400> 126
cttatagacc gatcgcacgg tctata                                      26

<210> 127
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for TylP repressor

<400> 127
atacaaaccg cgtcagcggt ttgtaa                                      26

<210> 128
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for MtrR repressor

<400> 128
tttttatccg tgcaatcgtg tatgtat                                     27

<210> 129
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for FarA repressor

<400> 129
gatacgaacg ggacggacgg tttgcagc                                    28

<210> 130
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for IcaR Se repressor

<400> 130
acaacctaac taacgaaagg taggtgaa                                    28

<210> 131
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for ScbR repressor

<400> 131
gaaaaaaaac cgctctagtc tgtatctta                                   29

<210> 132
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for PhlF repressor

<400> 132
atgatacgaa acgtaccgta tcgttaaggt                                  30

<210> 133
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for SmeT repressor

<400> 133
gtttacaaac aaacaagcat gtatgtatat                                  30

<210> 134
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for MphR repressor

<400> 134
gaatataacc gacgtgactg ttacatttag g                                31

<210> 135
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for LuxT repressor

<400> 135
ttcggtttac tttgtttaga atacccacgt ct                               32

<210> 136
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for PsrA repressor

<400> 136
agcagggctg aaacgtatgt ttcaaacacc tgtttctg                         38

<210> 137
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for TtgR repressor

<400> 137
cagcagtatt tacaaacaac catgaatgta agtatattcc                       40

<210> 138
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for VarR repressor

<400> 138
cacttgtaca tcgtataact ctcatatacg ttgtagaaca g                     41

<210> 139
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target DNA sequence operator for EthR repressor

<400> 139
gtgtcgatag tgtcgacatc tcgttgacgg cctcgacatt acgttgatag cgtgg      55

<210> 140
<211> 6
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic His-6 tag, amino-terminal 6x-His tag

<400> 140
His His His His His His
 1               5      


<210> 141
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic N-terminal His-6 tag with cleavable
      PreScission protease cleavage site

<400> 141
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Glu Val
 1               5                  10                  15      
Leu Phe Gln Gly Pro His
            20          


<210> 142
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic repressed pOR1 promoter

<400> 142
tttgacatac ctctggcggt gatatataat ggttgc                           36

<210> 143
<211> 289
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic upstream inducible promoter Pbad

<400> 143
agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt 60
ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa 120
agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga 180
ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga 240
tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccg             289

<210> 144
<211> 84
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic upstream inducible promoter Ptac

<400> 144
ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat 60
tgtgagcgga taacaatttc acac                                        84

<210> 145
<211> 78
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic upstream inducible promoter Modified-Ptac

<400> 145
ggcaaatatt ctgaaatgag ctgataaatg tgagcggata acattgacat tgtgagcgga 60
taacaagata ctgagcac                                               78

<210> 146
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic super-fold gfp ribosome binding sequence (SDA)

<400> 146
actagaagga ggaaaaaaat g                                           21

<210> 147
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic fused cI-gfp gene short linker sequence

<400> 147
ggcggtggcg gt                                                     12

<210> 148
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic ribosome binding sequence SDmut

<400> 148
actagaacca tgaaaaaagt g                                           21

<210> 149
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pD/E20J T5 bacteriophage 5' UTR spacer sequence

<400> 149
agttcgatga gagcgataac cctctacaaa taattttgtt taa                   43

<210> 150
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pH207J T5 bacteriophage 5' UTR spacer sequence

<400> 150
ataaattgat aaacaaaaac ctctacaaat aattttgttt aa                    42

<210> 151
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pN25J T5 bacteriophage 5' UTR spacer sequence

<400> 151
ataaatttga gagaggagtt cctctacaaa taattttgtt taa                   43

<210> 152
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pG25J T5 bacteriophage 5' UTR spacer sequence

<400> 152
attaaagagg agaaattaac cctctacaaa taattttgtt taa                   43

<210> 153
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pJ5J T5 bacteriophage 5' UTR spacer sequence

<400> 153
aaacctaatg gatcgacctt cctctacaaa taattttgtt taa                   43

<210> 154
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pA1J T7 bacteriophage 5' UTR spacer sequence

<400> 154
atcgagaggg acacggcgac ctctacaaat aattttgttt aa                    42

<210> 155
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pA2J T7 bacteriophage 5' UTR spacer sequence

<400> 155
gctaggtaac actagcagcc ctctacaaat aattttgttt aa                    42

<210> 156
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pA3J T7 bacteriophage 5' UTR spacer sequence

<400> 156
atgaaacgac agtgagtcac ctctacaaat aattttgttt aa                    42

<210> 157
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic phi10J T7 bacteriophage 5' UTR spacer sequence

<400> 157
agggagacca caacggtttc cctctacaaa taattttgtt taa                   43

<210> 158
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic DG146aJ high-transcription escape 5' UTR spacer
      sequence

<400> 158
attaaaaaac ctgctaggat cctctacaaa taattttgtt taa                   43

<210> 159
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic DG122J high-transcription escape 5' UTR spacer
      sequence

<400> 159
ataaaggaaa acggtcaggt cctctacaaa taattttgtt taa                   43

<210> 160
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic DG131aJ high-transcription escape 5' UTR spacer
      sequence

<400> 160
ataggttaaa agcctgtcat cctctacaaa taattttgtt taa                   43

<210> 161
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic MhpR3hppJ carbon utilization 5' UTR spacer sequence

<400> 161
acaataaaaa atcatttaca tgtttcctct acaaataatt ttgtttaa              48

<210> 162
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic GlcCJ carbon utilization 5' UTR spacer sequence

<400> 162
agaagcagcg cgcaaaaatc agctgcctct acaaataatt ttgtttaa              48

<210> 163
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic FucRPfucPJ carbon utilization 5' UTR spacer
      sequence

<400> 163
atgagttcat ttcagacagg caaatcctct acaaataatt ttgtttaa              48

<210> 164
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic FuncRPfucAJ carbon utilization 5' UTR spacer
      sequence

<400> 164
aacttgcagt tatttactgt gattacctct acaaataatt ttgtttaa              48

<210> 165
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic ChbRNagCJ carbon utilization 5' UTR spacer
      sequence

<400> 165
agccacaaaa aaagtcatgt tggttcctct acaaataatt ttgtttaa              48

<210> 166
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic AraCJ carbon utilization 5' UTR spacer sequence

<400> 166
acacagtcac ttatctttta gttaaaaggt cctctacaaa taattttgtt taa        53

<210> 167
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic AntiescapeJ anti-escaping sequence 5' UTR spacer
      sequence

<400> 167
atccggaatc ctcttcccgg cctctacaaa taattttgtt taa                   43

<210> 168
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic LeiQiJ 5' UTR spacer sequence

<400> 168
aacaaaataa aaaggagtcg ctcaccctct acaaataatt ttgtttaa              48

<210> 169
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pD/E20RBS T5 bacteriophage 5' UTR spacer sequence

<400> 169
agttcgatga gagcgataac agttccagat tcaggaacta taa                   43

<210> 170
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pH207RBS T5 bacteriophage 5' UTR spacer sequence

<400> 170
ataaattgat aaacaaaaaa gttccagatt caggaactat aa                    42

<210> 171
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pN25RBS T5 bacteriophage 5' UTR spacer sequence

<400> 171
ataaatttga gagaggagtt agttccagat tcaggaacta taa                   43

<210> 172
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pG25RBS T5 bacteriophage 5' UTR spacer sequence

<400> 172
attaaagagg agaaattaac agttccagat tcaggaacta taa                   43

<210> 173
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pJ5RBS T5 bacteriophage 5' UTR spacer sequence

<400> 173
aaacctaatg gatcgacctt agttccagat tcaggaacta taa                   43

<210> 174
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pA1RBS T7 bacteriophage 5' UTR spacer sequence

<400> 174
atcgagaggg acacggcgaa gttccagatt caggaactat aa                    42

<210> 175
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pA2RBS T7 bacteriophage 5' UTR spacer sequence

<400> 175
gctaggtaac actagcagca gttccagatt caggaactat aa                    42

<210> 176
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pA3RBS T7 bacteriophage 5' UTR spacer sequence

<400> 176
atgaaacgac agtgagtcaa gttccagatt caggaactat aa                    42

<210> 177
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic phi10RBS T7 bacteriophage 5' UTR spacer sequence

<400> 177
agggagacca caacggtttc agttccagat tcaggaacta taa                   43

<210> 178
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic DG146aRBS high-transcription escape 5' UTR spacer
      sequence

<400> 178
attaaaaaac ctgctaggat agttccagat tcaggaacta taa                   43

<210> 179
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic DG122RBS high-transcription escape 5' UTR spacer
      sequence

<400> 179
ataaaggaaa acggtcaggt agttccagat tcaggaacta taa                   43

<210> 180
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic DG131aRBS high-transcription escape 5' UTR spacer
      sequence

<400> 180
ataggttaaa agcctgtcat agttccagat tcaggaacta taa                   43

<210> 181
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic MhpR3hppRBS carbon utilization 5' UTR spacer
      sequence

<400> 181
acaataaaaa atcatttaca tgtttagttc cagattcagg aactataa              48

<210> 182
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic GlcCRBS carbon utilization 5' UTR spacer sequence

<400> 182
agaagcagcg cgcaaaaatc agctgagttc cagattcagg aactataa              48

<210> 183
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic FucRPfucPRBS carbon utilization 5' UTR spacer
      sequence

<400> 183
atgagttcat ttcagacagg caaatagttc cagattcagg aactataa              48

<210> 184
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic FuncRPfucARBS carbon utilization 5' UTR spacer
      sequence

<400> 184
aacttgcagt tatttactgt gattaagttc cagattcagg aactataa              48

<210> 185
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic ChbRNagCRBS carbon utilization 5' UTR spacer
      sequence

<400> 185
agccacaaaa aaagtcatgt tggttagttc cagattcagg aactataa              48

<210> 186
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic AraCRBS carbon utilization 5' UTR spacer sequence

<400> 186
acacagtcac ttatctttta gttaaaaggt agttccagat tcaggaacta taa        53

<210> 187
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic AntiescapeRBS anti-escaping sequence 5' UTR spacer
      sequence

<400> 187
atccggaatc ctcttcccgg agttccagat tcaggaacta taa                   43

<210> 188
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic LeiQiRBS 5' UTR spacer sequence

<400> 188
aacaaaataa aaaggagtcg ctcacagttc cagattcagg aactataa              48

<210> 189
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic OmpA4 hairpin stem loop 5' UTR spacer sequence

<400> 189
gatcaccagg gggatccccc ggtgaaggat                                  30

<210> 190
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic OmpA952 hairpin stem loop 5' UTR spacer sequence

<400> 190
gatcgcccac cggcagctgc cggtgggcga tcaaggat                         38

<210> 191
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic OmpA29 hairpin stem loop 5' UTR spacer sequence

<400> 191
gatcatcggt agagttaata ttgagcagat cccccggtga aggat                 45

<210> 192
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic papA hairpin stem loop 5' UTR spacer sequence

<400> 192
attgatctgg ttattaaagg taatcgggtc atttta                           36

<210> 193
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pufBA hairpin stem loop 5' UTR spacer sequence

<400> 193
gttctccacg ggtgggatga gcccctcgtg gtggaaatgc g                     41

<210> 194
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic gene32 hairpin stem loop 5' UTR spacer sequence

<400> 194
agcatgaggt aaagtgtcat gcaccaa                                     27

<210> 195
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pHP4 hairpin stem loop 5' UTR spacer sequence

<400> 195
acgtcgactt atctcgagtg agatattgtt gacggtac                         38

<210> 196
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pHP10 hairpin stem loop 5' UTR spacer sequence

<400> 196
acgtcgactt atctcgagtg agataagttg acggtac                          37

<210> 197
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pHP17 hairpin stem loop 5' UTR spacer sequence

<400> 197
acgtcgactt atctcgagac tgcagttcaa tagagatatt gttgacggta c          51

<210> 198
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic Ribo50 hairpin stem loop (ribozyme) 5' UTR spacer
      sequence

<400> 198
gactgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac ag         52

<210> 199
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic OmpA952J hairpin stem loop 5' UTR spacer sequence

<400> 199
gatcaccagg gggatccccc ggtgaaggat cctctacaaa taattttgtt taa        53

<210> 200
<211> 61
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic OmpA64J hairpin stem loop 5' UTR spacer sequence

<400> 200
gatcgcccac cggcagctgc cggtgggcga tcaaggatcc tctacaaata attttgttta 60
a                                                                 61

<210> 201
<211> 68
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic OmpA29J hairpin stem loop 5' UTR spacer sequence

<400> 201
gatcatcggt agagttaata ttgagcagat cccccggtga aggatcctct acaaataatt 60
ttgtttaa                                                          68

<210> 202
<211> 59
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic papAJ hairpin stem loop 5' UTR spacer sequence

<400> 202
attgatctgg ttattaaagg taatcgggtc attttacctc tacaaataat tttgtttaa  59

<210> 203
<211> 64
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pufBAJ hairpin stem loop 5' UTR spacer sequence

<400> 203
gttctccacg ggtgggatga gcccctcgtg gtggaaatgc gcctctacaa ataattttgt 60
ttaa                                                              64

<210> 204
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic gene32 hairpin stem loop 5' UTR spacer sequence

<400> 204
agcatgaggt aaagtgtcat gcaccaacct ctacaaataa ttttgtttaa            50

<210> 205
<211> 61
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pHP4J hairpin stem loop 5' UTR spacer sequence

<400> 205
acgtcgactt atctcgagtg agatattgtt gacggtaccc tctacaaata attttgttta 60
a                                                                 61

<210> 206
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pHP10J hairpin stem loop 5' UTR spacer sequence

<400> 206
acgtcgactt atctcgagtg agataagttg acggtaccct ctacaaataa ttttgtttaa 60


<210> 207
<211> 74
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pHP17J hairpin stem loop 5' UTR spacer sequence

<400> 207
acgtcgactt atctcgagac tgcagttcaa tagagatatt gttgacggta ccctctacaa 60
ataattttgt ttaa                                                   74

<210> 208
<211> 75
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic RiboJ hairpin stem loop (ribozyme) 5' UTR spacer
      sequence

<400> 208
gactgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac agcctctaca 60
aataattttg tttaa                                                  75

<210> 209
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic LtsvJ ribozyme

<400> 209
agtacgtctg agcgtgatac ccgctcactg aagatggccc ggtagggccg aaacgtacct 60
ctacaaataa ttttgtttaa                                             80

<210> 210
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic SccJ ribozyme

<400> 210
agatgctgta gtgggatgtg tgtctcacct gaagagtaca aaagtccgaa acggtatcct 60
ctacaaataa ttttgtttaa                                             80

<210> 211
<211> 75
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic RiboJ ribozyme

<400> 211
agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac agcctctaca 60
aataattttg tttaa                                                  75

<210> 212
<211> 79
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic SarJ ribozyme

<400> 212
agactgtcgc cggatgtgta tccgacctga cgatggccca aaagggccga aacagtcctc 60
tacaaataat tttgtttaa                                              79

<210> 213
<211> 81
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PlmJ ribozyme

<400> 213
agtcataagt ctgggctaag cccactgatg agtcgctgaa atgcgacgaa acttatgacc 60
tctacaaata attttgttta a                                           81

<210> 214
<211> 75
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic VtmoJ ribozyme

<400> 214
agtccgtagt ggatgtgtat ccactctgat gagtccgaaa ggacgaaacg gacctctaca 60
aataattttg tttaa                                                  75

<210> 215
<211> 86
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic ChmJ ribozyme

<400> 215
agaagaggtc ggcacctgac gtcggtgtcc tgatgaagat ccatgacagg atcgaaacct 60
cttcctctac aaataatttt gtttaa                                      86

<210> 216
<211> 78
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic ScvmJ ribozyme

<400> 216
agtactgtcg ccagacgtgg acccggcctg atgagtccga aaggacgaaa cagtacctct 60
acaaataatt ttgtttaa                                               78

<210> 217
<211> 85
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic SltJ ribozyme

<400> 217
aggacgtatg agactgactg aaacgccgtc tcactgatga ggccatggca ggccgaaacg 60
tccctctaca aataattttg tttaa                                       85

<210> 218
<211> 79
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PlmvJ ribozyme

<400> 218
aggaagagtc tgtgctaagc acactgacga gtctctgaga tgagacgaaa ctcttccctc 60
tacaaataat tttgtttaa                                              79

<210> 219
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pipaH9.8 promoter saturation mutagenesis
      forward primer

<220> 
<221> modified_base  
<222> (1)...(2)
<223> n = g, a, c or t

<400> 219
nnataaaaaa gtgctgaatt cac                                         23

<210> 220
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic pipaH9.8 promoter saturation mutagenesis
      reverse primer

<220> 
<221> modified_base  
<222> (1)...(2)
<223> n = g, a, c or t

<400> 220
nnataaggat aaacaaaatt ggctg                                       25

<210> 221
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf02 2817

<400> 221
catgacaaac aaaaacagat gcgttacgga actttacaaa aacgagacac tctaaccctt 60
tgcttgctca aattgcagct                                             80

<210> 222
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf03 UP1198

<400> 222
cagtacaaaa ttttttagat gcgtttttaa cttcgttcct tttcggcgtt ctaataacca 60
aagctcagaa ataatagatg                                             80

<210> 223
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf11 3726

<400> 223
atgcctccac accgctcgtc acatcctgtg atccactctt catcccgcta cgtaacacct 60
ctgcatcgcg aaccaaaacc                                             80

<210> 224
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf12 UP807

<400> 224
cagtacaaaa ttttttagat gcgttgcggg aatctccccg gccgatgggc cgtttcccag 60
gtcgagtggc ctgaatcgga                                             80

<210> 225
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf15 UP436

<400> 225
cagtacaaaa ttttttagat gcgttcttgg gaaccgaacg ccggtgcccg cgttcggttc 60
cggggatctt atcaactttt                                             80

<210> 226
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf16 3622

<400> 226
cttggatgaa aagaaaccca ccgacggtgt aaccctggcg gccgatgcaa cgaactaact 60
cacaggacgt gctcagcacc                                             80

<210> 227
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf17  UP1691

<400> 227
cagtacaaaa ttttttagat gcgtttggtg aaccaaactc ttactcgact cgtgtcagta 60
agcgggaggt gatcgcgtgg                                             80

<210> 228
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf18 UP1700

<400> 228
cagtacaaaa ttttttagat gcgtttgcat ccagattgtc tcggcggcgg taatgccata 60
agcaatgttc gatggcgcag                                             80

<210> 229
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf19 UP1315

<400> 229
cagtacaaaa ttttttagat gcgtttcctc ccgctcctgt ggagcacgat cgaacgcgaa 60
cgcggtcact atacccatgc                                             80

<210> 230
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf20 992

<400> 230
gcgcggataa aaatttcatt tgcccgcgac ggattccccg cccatctatc gttgaaccca 60
tcagctgcgt tcatcagcga                                             80

<210> 231
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf21 UP4014

<400> 231
cagtacaaaa ttttttagat gcgttaggca accctttttc atccggcttc gtctatatct 60
atagaaaccg acaccaaacc                                             80

<210> 232
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf22 UP1147

<400> 232
cagtacaaaa ttttttagat gcgttgttgt gaggaatcgc gctcctgcgc gaatcatccc 60
gtgtcgtccc ttcacctgcc                                             80

<210> 233
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf24 UP69

<400> 233
cagtacaaaa ttttttagat gcgttacgga acgcagtctt ttcgtctgta tcaactccaa 60
aattcatcgt gcctaaacat                                             80

<210> 234
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf25 UP4311

<400> 234
cagtacaaaa ttttttagat gcgtcgagga actcaaactg cgccattatc gtctagctaa 60
cagaggttct gcttgggagg                                             80

<210> 235
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf26 UP601

<400> 235
cagtacaaaa ttttttagat gcgtttggaa taaccggtcg cctccatccg tttacatacc 60
gaatcccggc agcgccggcc                                             80

<210> 236
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf29 UP371

<400> 236
cagtacaaaa ttttttagat gcgttccggg aaccttttgt cggcacgaac atccaattgg 60
cggatgaaac actttgtctg                                             80

<210> 237
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf30 2079

<400> 237
accatccgta tttttttagg gatttataaa acttttccat ggcgtgcgtc gtctaataaa 60
tgggaaggag gaaatgatgt                                             80

<210> 238
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf31 34

<400> 238
caatggctga aaagaattgt aaaaaagatg aacgcttttg aatccggtgt cgtctcataa 60
ggcagaaaaa caaaaaaggg                                             80

<210> 239
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf32 1122

<400> 239
gcgactttat ttaacagcgg catggccagg gaaccgatgc gtcaatcgca ccacacaatg 60
acaactgctc tcatcattga                                             80

<210> 240
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf33 375

<400> 240
taagttgaac aattttgcac cttccgtcga accgtccgct gcatcgaccc gaccaacctt 60
gcgagacggc ctttgagcgt                                             80

<210> 241
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf38 UP1322

<400> 241
cagtacaaaa ttttttagat gcgttgtaca accctcacgg gggtggacgt gtccaactgg 60
cgtggcagag gttctcgatt                                             80

<210> 242
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf39 UP1413

<400> 242
cagtacaaaa ttttttagat gcgtttgtca accgtccacg acgcgctggc gtctggaagg 60
gtgacccagc cagaccagcg                                             80

<210> 243
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf41 UP1141

<400> 243
cagtacaaaa ttttttagat gcgttcacgt cacaaacccg aaagctgaat cgtcatcccg 60
ttgaatccct caaacacgga                                             80

<210> 244
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic functional ECF promoter Pecf42 UP4062

<400> 244
cagtacaaaa ttttttagat gcgttcgctg tcgatccggc ccgtcgtcgt tcgtcgtacc 60
cccgagagcc cgcgaaaggc                                             80


