                         SEQUENCE LISTING

<110>  AURIGENE DISCOVERY TECHNOLOGIES LIMITED
 
<120>  THERAPEUTIC COMPOUNDS FOR IMMUNOMODULATION

<130>  NP-53; P162

<150>  1943/CHE/2011
<151>  2011-06-08

<150>  US 61/515,007
<151>  2011-08-04

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  137
<212>  PRT
<213>  Homo sapiens


<220>
<221>  PEPTIDE
<222>  (1)..(137)
<223>  Extracellular domain of Human PD1

<400>  1

Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp Asn 
1               5                   10                  15      


Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val Leu 
            20                  25                  30          


Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala Ala 
        35                  40                  45              


Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg Val 
    50                  55                  60                  


Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg Ala 
65                  70                  75                  80  


Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu Ala 
                85                  90                  95      


Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val Thr 
            100                 105                 110         


Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Arg 
        115                 120                 125             


Ser Ala Gly Gln Phe Gln Thr Leu Val 
    130                 135         


<210>  2
<211>  7
<212>  PRT
<213>  Homo sapiens


<220>
<221>  PEPTIDE
<222>  (1)..(7)
<223>  BC Loop

<400>  2

Ser Asn Thr Ser Glu Ser Phe 
1               5           


<210>  3
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2. 1,4-diaminobutane
       linked between delta C- terminal of Glu 5 and C- terminal of Phe 
       6.

<400>  3

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  4
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  1,4-diaminobutane linked between delta C-terminal of Glu 5 and 
       C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  N-acetylation of Ser 1;  Alpha C-terminal of Glu 5 is modified 
       with NH2.

<400>  4

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  5
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  CH3(CH2)14CO is attached to N-terminal of Ser 1;  Alpha 
       C-terminal of Glu 5 is modified with NH2.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  1,4-diaminobutane linked between delta C-terminal of Glu 5 and 
       C-terminal of Phe 6.

<400>  5

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  6
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  3-Maleimidopropanoic acid  is attached to N-terminal of Ser 7.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6.

<400>  6

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  7
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  (8-(3-maleimido propanamido)-3.6-dioxa octanoic acid) is attached
       to N-terminal of Ser 7.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C- terminal of Phe 
       6.

<400>  7

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  8
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C- terminal of Phe 
       6;  Phe 6 is D amino acid.

<400>  8

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  9
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  1,4-diaminobutane linked between delta C-terminal of Glu 5 and 
       C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 4th position of 
       phenyl in Phe 6 is substituted with amino methyl group.

<400>  9

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  10
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C- terminal of Phe 
       6; Ser 1 is D amino acid.

<400>  10

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  11
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6;
       Ser 7 is D amino acid.

<400>  11

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  12
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  1,4-diaminobutane linked between delta C-terminal of Glu 5 and 
       C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2;  4th position of 
       phenyl in Phe 6 is substituted with acetamide; Phe 6 is D amino 
       acid.

<400>  12

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  13
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6;
       Asn 2 is D amino acid.

<400>  13

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  14
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6;
       Glu 5 is D amino acid.

<400>  14

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  15
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  4th position of phenyl in Phe 6 is substituted with urea group; 
       Phe 6 is D amino acid

<400>  15

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  16
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  4th position of phenyl in Phe 6 is substituted with acetamide 
       group

<400>  16

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  17
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6;
       All amino acids are D amino acids.

<400>  17

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  18
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha carboxylic acid of Glu 3 is linked to NH2 group of Ser 4; 
       C-terminal of Ser 7 is modified NH2 group; All amino acids are 
       D-amino acid.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  1,4-diaminobutane linked between C-terminal of Ser 2 and delta 
       C-terminal of Glu 3.

<400>  18

Phe Ser Glu Ser Thr Asn Ser 
1               5           


<210>  19
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha carboxylic acid of Glu 3 is linked to NH2 group of Ser 4; 
       C-terminal of Ser 7 is modified NH2 group.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  1,4-diaminobutane linked between C-terminal of Ser 2 and delta 
       C-terminal of Glu 3

<400>  19

Phe Ser Glu Ser Thr Asn Ser 
1               5           


<210>  20
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  1,4-diaminobutane linked between delta C-terminal of Glu 5 and 
       C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  CH3(CH2)8CO- is linked to hydroxy group of Ser 1; Alpha 
       C-terminal of Glu 5 is modified with NH2.

<400>  20

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  21
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  (-CONH-) bond between Ser1 and Asn2 is reduced to (-CH2NH-).

<400>  21

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  22
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  (-CONH-) bond between Ser1 and Asn2 is reduced to (-CH2N-) 
       wherein this reduced amide is substituted with CH3(CH2)8CO-.

<400>  22

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  23
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  (-CONH-) bond between Ser1 and Asn2 is reduced to (-CH2N-) 
       wherein this reduced amide is substituted with CH3(CH2)4CO-.

<400>  23

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  24
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  (-CONH-) bond between Ser1 and Asn2 is reduced to (-CH2N-) 
       wherein this reduced amide is substituted with CH3CO-.

<400>  24

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  25
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  (-CONH-) bond between Asn 2 and Thr 3 is reduced to (-CH2NH-).

<400>  25

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  26
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  (-CONH-) bond between Asn2 and Thr 3 is reduced to (-CH2N-) 
       wherein this reduced amide is substituted with CH3CO-.

<400>  26

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  27
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  (-CONH-) bond between Asn 2 and Thr 3 is reduced to (-CH2N-) 
       wherein this reduced amide is substituted with (CH3)2CHCH2OCO-.

<400>  27

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  28
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Alpha C-terminal of Glu 5 is modified with NH2; 1,4-diaminobutane
       linked between delta C-terminal of Glu 5 and C-terminal of Phe 6.

<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  (-CONH-) bond between Ser 1 and Asn 2 is reduced to (-CH2N-) 
       wherein this reduced amide is substituted with (CH3)2CHCH2OCO-.

<400>  28

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  29
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  1,4-diaminobutane linked between delta C-terminal of Glu 5 and 
       C-terminal of Phe 6; N terminal of Glu 5 is bonded to C-terminal 
       of Ser 4.

<400>  29

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  30
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  2,6-diaminohexanoic acid is linked between delta C-terminal of 
       Glu 5 and N-terminal of Phe 6; N-terminal of Glu 5 is bonded to 
       C-terminal of Ser 4; C-terminal of Ser 7 is modified NH2.

<400>  30

Ser Asn Thr Ser Glu Phe Ser 
1               5           


<210>  31
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(6)
<223>  2,6-diaminohexanoic acid is linked between delta C-terminal of 
       Glu 4 and N-terminal of Phe 5; N-terminal of Glu 4 is bonded to 
       C-terminal of Ser 3; C-terminal of Ser 6 is modified NH2.

<400>  31

Asn Thr Ser Glu Phe Ser 
1               5       


<210>  32
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(5)
<223>  2,6-diaminohexanoic acid is linked between delta C-terminal of 
       Glu 3 and N-terminal of Phe 4; N-terminal of Glu 3 is bonded to 
       C-terminal of Ser 2; C-terminal of Ser 5 is modified NH2.

<400>  32

Thr Ser Glu Phe Ser 
1               5   


<210>  33
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  2,6-diaminohexanoic acid is linked between delta C-terminal of 
       Glu 2 and N-terminal of Phe 3; N-terminal of Glu 2 is bonded to 
       C-terminal of Ser 1; C-terminal of Ser 4 is modified NH2.

<400>  33

Ser Glu Phe Ser 
1               


