                         SEQUENCE LISTING

<110>  BASF Plant Science Company GmbH
 
<120>  Nuclease fusion protein and uses thereof

<130>  BPS71758EP

<160>  13    

<170>  PatentIn version 3.3

<210>  1
<211>  235
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  1

Met Lys Asn Ile Lys Lys Asn Gln Val Met Asn Leu Gly Pro Asn Ser 
1               5                   10                  15      


Lys Leu Leu Lys Glu Tyr Lys Ser Gln Leu Ile Glu Leu Asn Ile Glu 
            20                  25                  30          


Gln Phe Glu Ala Gly Ile Gly Leu Ile Leu Gly Asp Ala Tyr Ile Arg 
        35                  40                  45              


Ser Arg Asp Glu Gly Lys Thr Tyr Cys Met Gln Phe Glu Trp Lys Asn 
    50                  55                  60                  


Lys Ala Tyr Met Asp His Val Cys Leu Leu Tyr Asp Gln Trp Val Leu 
65                  70                  75                  80  


Ser Pro Pro His Lys Lys Glu Arg Val Asn His Leu Gly Asn Leu Val 
                85                  90                  95      


Ile Thr Trp Gly Ala Gln Thr Phe Lys His Gln Ala Phe Asn Lys Leu 
            100                 105                 110         


Ala Asn Leu Phe Ile Val Asn Asn Lys Lys Thr Ile Pro Asn Asn Leu 
        115                 120                 125             


Val Glu Asn Tyr Leu Thr Pro Met Ser Leu Ala Tyr Trp Phe Met Asp 
    130                 135                 140                 


Asp Gly Gly Lys Trp Asp Tyr Asn Lys Asn Ser Thr Asn Lys Ser Ile 
145                 150                 155                 160 


Val Leu Asn Thr Gln Ser Phe Thr Phe Glu Glu Val Glu Tyr Leu Val 
                165                 170                 175     


Lys Gly Leu Arg Asn Lys Phe Gln Leu Asn Cys Tyr Val Lys Ile Asn 
            180                 185                 190         


Lys Asn Lys Pro Ile Ile Tyr Ile Asp Ser Met Ser Tyr Leu Ile Phe 
        195                 200                 205             


Tyr Asn Leu Ile Lys Pro Tyr Leu Ile Pro Gln Met Met Tyr Lys Leu 
    210                 215                 220                 


Pro Asn Thr Ile Ser Ser Glu Thr Phe Leu Lys 
225                 230                 235 


<210>  2
<211>  159
<212>  PRT
<213>  Proteus vulgaris

<400>  2

Met Ser His Pro Asp Leu Asn Lys Leu Leu Glu Leu Trp Pro His Ile 
1               5                   10                  15      


Gln Glu Tyr Gln Asp Leu Ala Leu Lys His Gly Ile Asn Asp Ile Phe 
            20                  25                  30          


Gln Asp Asn Gly Gly Lys Leu Leu Gln Val Leu Leu Ile Thr Gly Leu 
        35                  40                  45              


Thr Val Leu Pro Gly Arg Glu Gly Asn Asp Ala Val Asp Asn Ala Gly 
    50                  55                  60                  


Gln Glu Tyr Glu Leu Lys Ser Ile Asn Ile Asp Leu Thr Lys Gly Phe 
65                  70                  75                  80  


Ser Thr His His His Met Asn Pro Val Ile Ile Ala Lys Tyr Arg Gln 
                85                  90                  95      


Val Pro Trp Ile Phe Ala Ile Tyr Arg Gly Ile Ala Ile Glu Ala Ile 
            100                 105                 110         


Tyr Arg Leu Glu Pro Lys Asp Leu Glu Phe Tyr Tyr Asp Lys Trp Glu 
        115                 120                 125             


Arg Lys Trp Tyr Ser Asp Gly His Lys Asp Ile Asn Asn Pro Lys Ile 
    130                 135                 140                 


Pro Val Lys Tyr Val Met Glu His Gly Thr Lys Ile Tyr Gly Ser 
145                 150                 155                 


<210>  3
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  linker sequence

<400>  3

Ala Ser Arg Thr Thr Gly 
1               5       


<210>  4
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  linker sequence

<400>  4

Ala Ser Thr Lys Gln Leu Val Lys Ser Gly 
1               5                   10  


<210>  5
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  linker sequence

<400>  5

Ala Ser Gly Gly Ser Gly Ser Gly Ser Gly 
1               5                   10  


<210>  6
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  linker sequence

<400>  6

Ala Ser Gly Asp Ser Gly Ser Asp Ser Gly 
1               5                   10  


<210>  7
<211>  1167
<212>  DNA
<213>  artificial

<220>
<223>  nucleic acid sequence PSs (NA) L0TY

<400>  7
atgggtcacc cagatctaaa taaattatta gagctttggc cgcatataca ggaatatcaa     60

gacttagcat taaaacatgg aataaatgat atttttcaag ataatggtgg aaagttgctt    120

caagtccttc taattacagg attaacagta ctaccaggac gagaaggtaa tgatgctgta    180

gataacgcag gacaagaata cgagttaaaa tcaataaaca tagacctcac taaaggtttt    240

tcaactcacc accacatgaa tcctgtaatt attgcaaaat atagacaagt accttggatt    300

tttgccatat accgtggtat cgcaatagaa gctatataca gattagagcc aaaagatcta    360

gaattttact atgataaatg ggaaaggaaa tggtattcag atgggcataa agatattaac    420

aaccctaaaa tacctgtaaa atatgtaatg gaacatggga caaagattta cgctagccgt    480

acgaccggta tgcataacat caaaaaaaac caggtaatga acctgggtcc gaactctaaa    540

ctgctgaaag aatacaaatc ccagctgatc gaactgaaca tcgaacagtt cgaagcaggt    600

atcggtctga tcctgggaaa cgcttacatc cgttcacgtg atgaaggtaa aacctactgt    660

atgcagttcg agtggaaaaa caaagcatac atggaccacg tatgtctgct gtacgatcag    720

tgggtactgt ccccgccgca caaaaaagaa cgtgttaacc acctgggtaa cctggtaatc    780

acctggggcg cccagacttt caaacaccaa gctttcaaca aactggctaa cctgttcatc    840

gttaacaaca aaaaaaccat cccgaacaac ctggttgaaa actacctgac cccgatgtct    900

ctggcatact ggttcatgga tgccggcggt aaatgggatt acaacaaaaa ctctaccaac    960

aaatcgatcg tactgaacac ccagtctttc actttcgaag aagtagaata cctggttaag   1020

ggtctgcgta acaaattcca actgaactgt tacgtaaaaa tcaacaaaaa caaaccgatc   1080

atctacatcg attctatgtc ttacctgatc ttctacaacc tgatcaaacc gtacctgatc   1140

ccacagatga tgtacaaact gccgaac                                       1167


<210>  8
<211>  1179
<212>  DNA
<213>  artificial

<220>
<223>  nucleic acid sequence PSs (NA) FokTY

<400>  8
atgggtcacc cagatctaaa taaattatta gagctttggc cgcatataca ggaatatcaa     60

gacttagcat taaaacatgg aataaatgat atttttcaag ataatggtgg aaagttgctt    120

caagtccttc taattacagg attaacagta ctaccaggac gagaaggtaa tgatgctgta    180

gataacgcag gacaagaata cgagttaaaa tcaataaaca tagacctcac taaaggtttt    240

tcaactcacc accacatgaa tcctgtaatt attgcaaaat atagacaagt accttggatt    300

tttgccatat accgtggtat cgcaatagaa gctatataca gattagagcc aaaagatcta    360

gaattttact atgataaatg ggaaaggaaa tggtattcag atgggcataa agatattaac    420

aaccctaaaa tacctgtaaa atatgtaatg gaacatggga caaagattta cgctagcacc    480

aaacagctgg ttaagtccgg tatgcataac atcaaaaaaa accaggtaat gaacctgggt    540

ccgaactcta aactgctgaa agaatacaaa tcccagctga tcgaactgaa catcgaacag    600

ttcgaagcag gtatcggtct gatcctggga aacgcttaca tccgttcacg tgatgaaggt    660

aaaacctact gtatgcagtt cgagtggaaa aacaaagcat acatggacca cgtatgtctg    720

ctgtacgatc agtgggtact gtccccgccg cacaaaaaag aacgtgttaa ccacctgggt    780

aacctggtaa tcacctgggg cgcccagact ttcaaacacc aagctttcaa caaactggct    840

aacctgttca tcgttaacaa caaaaaaacc atcccgaaca acctggttga aaactacctg    900

accccgatgt ctctggcata ctggttcatg gatgccggcg gtaaatggga ttacaacaaa    960

aactctacca acaaatcgat cgtactgaac acccagtctt tcactttcga agaagtagaa   1020

tacctggtta agggtctgcg taacaaattc caactgaact gttacgtaaa aatcaacaaa   1080

aacaaaccga tcatctacat cgattctatg tcttacctga tcttctacaa cctgatcaaa   1140

ccgtacctga tcccacagat gatgtacaaa ctgccgaac                          1179


<210>  9
<211>  389
<212>  PRT
<213>  artificial

<220>
<223>  protein PSs (NA) F0TY

<400>  9

Met Gly His Pro Asp Leu Asn Lys Leu Leu Glu Leu Trp Pro His Ile 
1               5                   10                  15      


Gln Glu Tyr Gln Asp Leu Ala Leu Lys His Gly Ile Asn Asp Ile Phe 
            20                  25                  30          


Gln Asp Asn Gly Gly Lys Leu Leu Gln Val Leu Leu Ile Thr Gly Leu 
        35                  40                  45              


Thr Val Leu Pro Gly Arg Glu Gly Asn Asp Ala Val Asp Asn Ala Gly 
    50                  55                  60                  


Gln Glu Tyr Glu Leu Lys Ser Ile Asn Ile Asp Leu Thr Lys Gly Phe 
65                  70                  75                  80  


Ser Thr His His His Met Asn Pro Val Ile Ile Ala Lys Tyr Arg Gln 
                85                  90                  95      


Val Pro Trp Ile Phe Ala Ile Tyr Arg Gly Ile Ala Ile Glu Ala Ile 
            100                 105                 110         


Tyr Arg Leu Glu Pro Lys Asp Leu Glu Phe Tyr Tyr Asp Lys Trp Glu 
        115                 120                 125             


Arg Lys Trp Tyr Ser Asp Gly His Lys Asp Ile Asn Asn Pro Lys Ile 
    130                 135                 140                 


Pro Val Lys Tyr Val Met Glu His Gly Thr Lys Ile Tyr Ala Ser Arg 
145                 150                 155                 160 


Thr Thr Gly Met His Asn Ile Lys Lys Asn Gln Val Met Asn Leu Gly 
                165                 170                 175     


Pro Asn Ser Lys Leu Leu Lys Glu Tyr Lys Ser Gln Leu Ile Glu Leu 
            180                 185                 190         


Asn Ile Glu Gln Phe Glu Ala Gly Ile Gly Leu Ile Leu Gly Asn Ala 
        195                 200                 205             


Tyr Ile Arg Ser Arg Asp Glu Gly Lys Thr Tyr Cys Met Gln Phe Glu 
    210                 215                 220                 


Trp Lys Asn Lys Ala Tyr Met Asp His Val Cys Leu Leu Tyr Asp Gln 
225                 230                 235                 240 


Trp Val Leu Ser Pro Pro His Lys Lys Glu Arg Val Asn His Leu Gly 
                245                 250                 255     


Asn Leu Val Ile Thr Trp Gly Ala Gln Thr Phe Lys His Gln Ala Phe 
            260                 265                 270         


Asn Lys Leu Ala Asn Leu Phe Ile Val Asn Asn Lys Lys Thr Ile Pro 
        275                 280                 285             


Asn Asn Leu Val Glu Asn Tyr Leu Thr Pro Met Ser Leu Ala Tyr Trp 
    290                 295                 300                 


Phe Met Asp Ala Gly Gly Lys Trp Asp Tyr Asn Lys Asn Ser Thr Asn 
305                 310                 315                 320 


Lys Ser Ile Val Leu Asn Thr Gln Ser Phe Thr Phe Glu Glu Val Glu 
                325                 330                 335     


Tyr Leu Val Lys Gly Leu Arg Asn Lys Phe Gln Leu Asn Cys Tyr Val 
            340                 345                 350         


Lys Ile Asn Lys Asn Lys Pro Ile Ile Tyr Ile Asp Ser Met Ser Tyr 
        355                 360                 365             


Leu Ile Phe Tyr Asn Leu Ile Lys Pro Tyr Leu Ile Pro Gln Met Met 
    370                 375                 380                 


Tyr Lys Leu Pro Asn 
385                 


<210>  10
<211>  393
<212>  PRT
<213>  artificial

<220>
<223>  Prt PSs (NA) FokTY

<400>  10

Met Gly His Pro Asp Leu Asn Lys Leu Leu Glu Leu Trp Pro His Ile 
1               5                   10                  15      


Gln Glu Tyr Gln Asp Leu Ala Leu Lys His Gly Ile Asn Asp Ile Phe 
            20                  25                  30          


Gln Asp Asn Gly Gly Lys Leu Leu Gln Val Leu Leu Ile Thr Gly Leu 
        35                  40                  45              


Thr Val Leu Pro Gly Arg Glu Gly Asn Asp Ala Val Asp Asn Ala Gly 
    50                  55                  60                  


Gln Glu Tyr Glu Leu Lys Ser Ile Asn Ile Asp Leu Thr Lys Gly Phe 
65                  70                  75                  80  


Ser Thr His His His Met Asn Pro Val Ile Ile Ala Lys Tyr Arg Gln 
                85                  90                  95      


Val Pro Trp Ile Phe Ala Ile Tyr Arg Gly Ile Ala Ile Glu Ala Ile 
            100                 105                 110         


Tyr Arg Leu Glu Pro Lys Asp Leu Glu Phe Tyr Tyr Asp Lys Trp Glu 
        115                 120                 125             


Arg Lys Trp Tyr Ser Asp Gly His Lys Asp Ile Asn Asn Pro Lys Ile 
    130                 135                 140                 


Pro Val Lys Tyr Val Met Glu His Gly Thr Lys Ile Tyr Ala Ser Thr 
145                 150                 155                 160 


Lys Gln Leu Val Lys Ser Gly Met His Asn Ile Lys Lys Asn Gln Val 
                165                 170                 175     


Met Asn Leu Gly Pro Asn Ser Lys Leu Leu Lys Glu Tyr Lys Ser Gln 
            180                 185                 190         


Leu Ile Glu Leu Asn Ile Glu Gln Phe Glu Ala Gly Ile Gly Leu Ile 
        195                 200                 205             


Leu Gly Asn Ala Tyr Ile Arg Ser Arg Asp Glu Gly Lys Thr Tyr Cys 
    210                 215                 220                 


Met Gln Phe Glu Trp Lys Asn Lys Ala Tyr Met Asp His Val Cys Leu 
225                 230                 235                 240 


Leu Tyr Asp Gln Trp Val Leu Ser Pro Pro His Lys Lys Glu Arg Val 
                245                 250                 255     


Asn His Leu Gly Asn Leu Val Ile Thr Trp Gly Ala Gln Thr Phe Lys 
            260                 265                 270         


His Gln Ala Phe Asn Lys Leu Ala Asn Leu Phe Ile Val Asn Asn Lys 
        275                 280                 285             


Lys Thr Ile Pro Asn Asn Leu Val Glu Asn Tyr Leu Thr Pro Met Ser 
    290                 295                 300                 


Leu Ala Tyr Trp Phe Met Asp Ala Gly Gly Lys Trp Asp Tyr Asn Lys 
305                 310                 315                 320 


Asn Ser Thr Asn Lys Ser Ile Val Leu Asn Thr Gln Ser Phe Thr Phe 
                325                 330                 335     


Glu Glu Val Glu Tyr Leu Val Lys Gly Leu Arg Asn Lys Phe Gln Leu 
            340                 345                 350         


Asn Cys Tyr Val Lys Ile Asn Lys Asn Lys Pro Ile Ile Tyr Ile Asp 
        355                 360                 365             


Ser Met Ser Tyr Leu Ile Phe Tyr Asn Leu Ile Lys Pro Tyr Leu Ile 
    370                 375                 380                 


Pro Gln Met Met Tyr Lys Leu Pro Asn 
385                 390             


<210>  11
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tripartite DNA recognition site

<400>  11
tagggataac agggtaatgg tactcagctg attcatatta ccctgttatc ccta           54


<210>  12
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  tripartite DNA recognition site

<400>  12
tagggataac agggtaatat gaatcagctg agtaccatta ccctgttatc ccta           54


<210>  13
<211>  54
<212>  DNA
<213>  artificial

<220>
<223>  tripartite DNA recognition site


<220>
<221>  misc_feature
<222>  (19)..(24)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (31)..(36)
<223>  n is a, c, g, or t

<400>  13
tagggataac agggtaatnn nnnncagctg nnnnnnatta ccctgttatc ccta           54


