                         SEQUENCE LISTING

<110>  Cargill, Incorporated
       Pemble, Trent H
       Porter, Michael A
       Solheid, Christopher
       Brent, Hilbert H
       AnaLisa, Dickey
       Dustin, Johnson P
 
<120>  Increasing Product Yield and/or Throughput by Supersaturation of 
       Substrate

<130>  N00125USP1

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  861
<212>  DNA
<213>  Unknown

<220>
<223>  Obtained from environmental sample

<400>  1
atggacgcac tgggatatta caacggaaat tgggggcctc tggacgagat gactgtgccg       60

atgaacgaca ggggctgcta ctttggagac ggcgtatacg acgctacctg cgccgttaac      120

ggagttattt ttgccctgga tgagcacatt gacaggtttt tcaacagcgc aaagctcctg      180

gaaataaata ttagcttaac aaaagaggaa ttgaaaaaaa ctttaaatga aatgtactcc      240

aaagtggata aaggagagta cctggtttat tggcaggtga ctcgcggaac aggccggcga      300

agccatgtat ttccggcagg cccttcaaat ctctggatta taattaagcc caatcacatc      360

gacaatcttt atagaaaaat caagctcatt accatggatg atacccgctt cctacactgc      420

aacatcaaga cccttaacct tatacccaat gtcattgctt cccagcgggc gctggaagcg      480

ggttgccacg aggcggtatt tcaccggggc gaaacggtaa ccgagtgcgc tcacagcaat      540

gtccatatca ttaaaaacgg caggtttatt acccatccgg cggacaacct gatcctccgg      600

ggtacagccc gcagtcattt attgcaggcc tgtgtcaggc ttaacattcc ggtagacgaa      660

cgggaatttt ccctttcgga attattcgac gcggacgagg tgcttgtgtc cagcagcggc      720

acactcggcc ttagcgccga agaaatcgac ggaaaaaaag cgggagggaa agcgcctgaa      780

ctgctaaaaa aaatccagga tgaagtgctg agggaattta tcgaagcgac aggctacacg      840

cctgagtgga gcagggtata g                                                861


<210>  2
<211>  286
<212>  PRT
<213>  Unknown

<220>
<223>  Obtained from environmental sample


<220>
<221>  DOMAIN
<222>  (8)..(270)
<223>  Aminotransferase class IV

<220>
<221>  SITE
<222>  (63)..(66)
<223>  N-glycosylation site. Prosite id = PS00001

<400>  2

Met Asp Ala Leu Gly Tyr Tyr Asn Gly Asn Trp Gly Pro Leu Asp Glu 
1               5                   10                  15      


Met Thr Val Pro Met Asn Asp Arg Gly Cys Tyr Phe Gly Asp Gly Val 
            20                  25                  30          


Tyr Asp Ala Thr Cys Ala Val Asn Gly Val Ile Phe Ala Leu Asp Glu 
        35                  40                  45              


His Ile Asp Arg Phe Phe Asn Ser Ala Lys Leu Leu Glu Ile Asn Ile 
    50                  55                  60                  


Ser Leu Thr Lys Glu Glu Leu Lys Lys Thr Leu Asn Glu Met Tyr Ser 
65                  70                  75                  80  


Lys Val Asp Lys Gly Glu Tyr Leu Val Tyr Trp Gln Val Thr Arg Gly 
                85                  90                  95      


Thr Gly Arg Arg Ser His Val Phe Pro Ala Gly Pro Ser Asn Leu Trp 
            100                 105                 110         


Ile Ile Ile Lys Pro Asn His Ile Asp Asn Leu Tyr Arg Lys Ile Lys 
        115                 120                 125             


Leu Ile Thr Met Asp Asp Thr Arg Phe Leu His Cys Asn Ile Lys Thr 
    130                 135                 140                 


Leu Asn Leu Ile Pro Asn Val Ile Ala Ser Gln Arg Ala Leu Glu Ala 
145                 150                 155                 160 


Gly Cys His Glu Ala Val Phe His Arg Gly Glu Thr Val Thr Glu Cys 
                165                 170                 175     


Ala His Ser Asn Val His Ile Ile Lys Asn Gly Arg Phe Ile Thr His 
            180                 185                 190         


Pro Ala Asp Asn Leu Ile Leu Arg Gly Thr Ala Arg Ser His Leu Leu 
        195                 200                 205             


Gln Ala Cys Val Arg Leu Asn Ile Pro Val Asp Glu Arg Glu Phe Ser 
    210                 215                 220                 


Leu Ser Glu Leu Phe Asp Ala Asp Glu Val Leu Val Ser Ser Ser Gly 
225                 230                 235                 240 


Thr Leu Gly Leu Ser Ala Glu Glu Ile Asp Gly Lys Lys Ala Gly Gly 
                245                 250                 255     


Lys Ala Pro Glu Leu Leu Lys Lys Ile Gln Asp Glu Val Leu Arg Glu 
            260                 265                 270         


Phe Ile Glu Ala Thr Gly Tyr Thr Pro Glu Trp Ser Arg Val 
        275                 280                 285     


<210>  3
<211>  690
<212>  DNA
<213>  Unknown

<220>
<223>  Obtained from environmental sample

<400>  3
atgcctatcg ttgttacgaa gatcgaccga cccagcgcgg cggacgtcga aaggatcgcc       60

gcctatggtg tcgcgacctt gcatgaagcg caaggacgaa ccgggttgat ggcgtccaat      120

atgcgcccaa tctatcgccc tgcgcacatt gccgggcccg cggtgacctg ccttgtggcg      180

cctggcgaca attggatgat ccatgtcgcc gtcgaacagt gccagccggg agatgtcctg      240

gtcgtggtac cgaccagccc ctgcgaagac ggctatttcg gcgatctgct ggcgacctcg      300

ctgcggtcgc gcggggtcaa aggtctgatc atcgaggccg gcgtacgcga tatcgcgaca      360

ttgaccgaga tgaaattccc ggtctggtcc aaggcggtgt tcgcgcaagg aacggtcaag      420

gagaccatcg ccagcgtcaa tgtgcccctc gtctgcgcgg gcgcccgcat cgtgccgggc      480

gatctgatcg ttgccgacga cgacggggtc gtcgtgattc caagacgttc cgttccggcg      540

gtcctttcca gcgccgaggc ccgcgaagag aaggaagccc gcaaccgcgc ccgcttcgaa      600

gctggcgagc tgggcctcga cgtctacaac atgcgccagc gcctggccga caagggcttg      660

cgctatgtcg agcggctgcc cgaggaatag                                       690


<210>  4
<211>  229
<212>  PRT
<213>  Unknown

<220>
<223>  Obtained from environmental sample


<220>
<221>  DOMAIN
<222>  (20)..(172)
<223>  Demethylmenaquinone methyltransferase

<400>  4

Met Pro Ile Val Val Thr Lys Ile Asp Arg Pro Ser Ala Ala Asp Val 
1               5                   10                  15      


Glu Arg Ile Ala Ala Tyr Gly Val Ala Thr Leu His Glu Ala Gln Gly 
            20                  25                  30          


Arg Thr Gly Leu Met Ala Ser Asn Met Arg Pro Ile Tyr Arg Pro Ala 
        35                  40                  45              


His Ile Ala Gly Pro Ala Val Thr Cys Leu Val Ala Pro Gly Asp Asn 
    50                  55                  60                  


Trp Met Ile His Val Ala Val Glu Gln Cys Gln Pro Gly Asp Val Leu 
65                  70                  75                  80  


Val Val Val Pro Thr Ser Pro Cys Glu Asp Gly Tyr Phe Gly Asp Leu 
                85                  90                  95      


Leu Ala Thr Ser Leu Arg Ser Arg Gly Val Lys Gly Leu Ile Ile Glu 
            100                 105                 110         


Ala Gly Val Arg Asp Ile Ala Thr Leu Thr Glu Met Lys Phe Pro Val 
        115                 120                 125             


Trp Ser Lys Ala Val Phe Ala Gln Gly Thr Val Lys Glu Thr Ile Ala 
    130                 135                 140                 


Ser Val Asn Val Pro Leu Val Cys Ala Gly Ala Arg Ile Val Pro Gly 
145                 150                 155                 160 


Asp Leu Ile Val Ala Asp Asp Asp Gly Val Val Val Ile Pro Arg Arg 
                165                 170                 175     


Ser Val Pro Ala Val Leu Ser Ser Ala Glu Ala Arg Glu Glu Lys Glu 
            180                 185                 190         


Ala Arg Asn Arg Ala Arg Phe Glu Ala Gly Glu Leu Gly Leu Asp Val 
        195                 200                 205             


Tyr Asn Met Arg Gln Arg Leu Ala Asp Lys Gly Leu Arg Tyr Val Glu 
    210                 215                 220                 


Arg Leu Pro Glu Glu 
225                 


