                         SEQUENCE LISTING

<110>  Oxford Nanopore Technologies Limited
 
<120>  Coupling Method

<130>  N.112486C

<150>  61/490,860
<151>  2011-05-27

<150>  61/599,246
<151>  2012-02-15

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  558
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mycobacterium smegmatis porin A mutant 
       (D90N/D91N/D93N/D118R/D134R/E139K)

<400>  1
atgggtctgg ataatgaact gagcctggtg gacggtcaag atcgtaccct gacggtgcaa       60

caatgggata cctttctgaa tggcgttttt ccgctggatc gtaatcgcct gacccgtgaa      120

tggtttcatt ccggtcgcgc aaaatatatc gtcgcaggcc cgggtgctga cgaattcgaa      180

ggcacgctgg aactgggtta tcagattggc tttccgtggt cactgggcgt tggtatcaac      240

ttctcgtaca ccacgccgaa tattctgatc aacaatggta acattaccgc accgccgttt      300

ggcctgaaca gcgtgattac gccgaacctg tttccgggtg ttagcatctc tgcccgtctg      360

ggcaatggtc cgggcattca agaagtggca acctttagtg tgcgcgtttc cggcgctaaa      420

ggcggtgtcg cggtgtctaa cgcccacggt accgttacgg gcgcggccgg cggtgtcctg      480

ctgcgtccgt tcgcgcgcct gattgcctct accggcgaca gcgttacgac ctatggcgaa      540

ccgtggaata tgaactaa                                                    558


<210>  2
<211>  184
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mycobacterium smegmatis porin A mutant 
       (D90N/D91N/D93N/D118R/D134R/E139K)

<400>  2

Gly Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu 
1               5                   10                  15      


Thr Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp 
            20                  25                  30          


Arg Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr 
        35                  40                  45              


Ile Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu 
    50                  55                  60                  


Gly Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe 
65                  70                  75                  80  


Ser Tyr Thr Thr Pro Asn Ile Leu Ile Asn Asn Gly Asn Ile Thr Ala 
                85                  90                  95      


Pro Pro Phe Gly Leu Asn Ser Val Ile Thr Pro Asn Leu Phe Pro Gly 
            100                 105                 110         


Val Ser Ile Ser Ala Arg Leu Gly Asn Gly Pro Gly Ile Gln Glu Val 
        115                 120                 125             


Ala Thr Phe Ser Val Arg Val Ser Gly Ala Lys Gly Gly Val Ala Val 
    130                 135                 140                 


Ser Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu 
145                 150                 155                 160 


Arg Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr 
                165                 170                 175     


Tyr Gly Glu Pro Trp Asn Met Asn 
            180                 


<210>  3
<211>  882
<212>  DNA
<213>  Artificial sequence

<220>
<223>  alpha-hemolysin mutant (M111R)

<400>  3
atggcagatt ctgatattaa tattaaaacc ggtactacag atattggaag caatactaca       60

gtaaaaacag gtgatttagt cacttatgat aaagaaaatg gcatgcacaa aaaagtattt      120

tatagtttta tcgatgataa aaatcacaat aaaaaactgc tagttattag aacaaaaggt      180

accattgctg gtcaatatag agtttatagc gaagaaggtg ctaacaaaag tggtttagcc      240

tggccttcag cctttaaggt acagttgcaa ctacctgata atgaagtagc tcaaatatct      300

gattactatc caagaaattc gattgataca aaagagtata ggagtacttt aacttatgga      360

ttcaacggta atgttactgg tgatgataca ggaaaaattg gcggccttat tggtgcaaat      420

gtttcgattg gtcatacact gaaatatgtt caacctgatt tcaaaacaat tttagagagc      480

ccaactgata aaaaagtagg ctggaaagtg atatttaaca atatggtgaa tcaaaattgg      540

ggaccatacg atcgagattc ttggaacccg gtatatggca atcaactttt catgaaaact      600

agaaatggtt ctatgaaagc agcagataac ttccttgatc ctaacaaagc aagttctcta      660

ttatcttcag ggttttcacc agacttcgct acagttatta ctatggatag aaaagcatcc      720

aaacaacaaa caaatataga tgtaatatac gaacgagttc gtgatgatta ccaattgcat      780

tggacttcaa caaattggaa aggtaccaat actaaagata aatggacaga tcgttcttca      840

gaaagatata aaatcgattg ggaaaaagaa gaaatgacaa at                         882


<210>  4
<211>  293
<212>  PRT
<213>  Artificial sequence

<220>
<223>  alpha-hemolysin mutant (M111R)

<400>  4

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Arg Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  5
<211>  1830
<212>  DNA
<213>  bacteriophage phi AR29

<400>  5
atgaaacaca tgccgcgtaa aatgtatagc tgcgcgtttg aaaccacgac caaagtggaa       60

gattgtcgcg tttgggccta tggctacatg aacatcgaag atcattctga atacaaaatc      120

ggtaacagtc tggatgaatt tatggcatgg gtgctgaaag ttcaggcgga tctgtacttc      180

cacaacctga aatttgatgg cgcattcatt atcaactggc tggaacgtaa tggctttaaa      240

tggagcgcgg atggtctgcc gaacacgtat aataccatta tctctcgtat gggccagtgg      300

tatatgattg atatctgcct gggctacaaa ggtaaacgca aaattcatac cgtgatctat      360

gatagcctga aaaaactgcc gtttccggtg aagaaaattg cgaaagattt caaactgacg      420

gttctgaaag gcgatattga ttatcacaaa gaacgtccgg ttggttacaa aatcaccccg      480

gaagaatacg catacatcaa aaacgatatc cagatcatcg cagaagcgct gctgattcag      540

tttaaacagg gcctggatcg catgaccgcg ggcagtgata gcctgaaagg tttcaaagat      600

atcatcacga ccaaaaaatt caaaaaagtg ttcccgacgc tgagcctggg tctggataaa      660

gaagttcgtt atgcctaccg cggcggtttt acctggctga acgatcgttt caaagaaaaa      720

gaaattggcg agggtatggt gtttgatgtt aatagtctgt atccggcaca gatgtacagc      780

cgcctgctgc cgtatggcga accgatcgtg ttcgagggta aatatgtttg ggatgaagat      840

tacccgctgc atattcagca catccgttgt gaatttgaac tgaaagaagg ctatattccg      900

accattcaga tcaaacgtag tcgcttctat aagggtaacg aatacctgaa aagctctggc      960

ggtgaaatcg cggatctgtg gctgagtaac gtggatctgg aactgatgaa agaacactac     1020

gatctgtaca acgttgaata catcagcggc ctgaaattta aagccacgac cggtctgttc     1080

aaagatttca tcgataaatg gacctacatc aaaacgacct ctgaaggcgc gattaaacag     1140

ctggccaaac tgatgctgaa cagcctgtat ggcaaattcg cctctaatcc ggatgtgacc     1200

ggtaaagttc cgtacctgaa agaaaatggc gcactgggtt ttcgcctggg cgaagaagaa     1260

acgaaagatc cggtgtatac cccgatgggt gttttcatta cggcctgggc acgttacacg     1320

accatcaccg cggcccaggc atgctatgat cgcattatct actgtgatac cgattctatt     1380

catctgacgg gcaccgaaat cccggatgtg attaaagata tcgttgatcc gaaaaaactg     1440

ggttattggg cccacgaaag tacgtttaaa cgtgcaaaat acctgcgcca gaaaacctac     1500

atccaggata tctacatgaa agaagtggat ggcaaactgg ttgaaggttc tccggatgat     1560

tacaccgata tcaaattcag tgtgaaatgc gccggcatga cggataaaat caaaaaagaa     1620

gtgaccttcg aaaacttcaa agttggtttc agccgcaaaa tgaaaccgaa accggtgcag     1680

gttccgggcg gtgtggttct ggtggatgat acgtttacca ttaaatctgg cggtagtgcg     1740

tggagccatc cgcagttcga aaaaggcggt ggctctggtg gcggttctgg cggtagtgcc     1800

tggagccacc cgcagtttga aaaataataa                                      1830


<210>  6
<211>  608
<212>  PRT
<213>  bacteriophage phi AR29

<400>  6

Met Lys His Met Pro Arg Lys Met Tyr Ser Cys Ala Phe Glu Thr Thr 
1               5                   10                  15      


Thr Lys Val Glu Asp Cys Arg Val Trp Ala Tyr Gly Tyr Met Asn Ile 
            20                  25                  30          


Glu Asp His Ser Glu Tyr Lys Ile Gly Asn Ser Leu Asp Glu Phe Met 
        35                  40                  45              


Ala Trp Val Leu Lys Val Gln Ala Asp Leu Tyr Phe His Asn Leu Lys 
    50                  55                  60                  


Phe Asp Gly Ala Phe Ile Ile Asn Trp Leu Glu Arg Asn Gly Phe Lys 
65                  70                  75                  80  


Trp Ser Ala Asp Gly Leu Pro Asn Thr Tyr Asn Thr Ile Ile Ser Arg 
                85                  90                  95      


Met Gly Gln Trp Tyr Met Ile Asp Ile Cys Leu Gly Tyr Lys Gly Lys 
            100                 105                 110         


Arg Lys Ile His Thr Val Ile Tyr Asp Ser Leu Lys Lys Leu Pro Phe 
        115                 120                 125             


Pro Val Lys Lys Ile Ala Lys Asp Phe Lys Leu Thr Val Leu Lys Gly 
    130                 135                 140                 


Asp Ile Asp Tyr His Lys Glu Arg Pro Val Gly Tyr Lys Ile Thr Pro 
145                 150                 155                 160 


Glu Glu Tyr Ala Tyr Ile Lys Asn Asp Ile Gln Ile Ile Ala Glu Ala 
                165                 170                 175     


Leu Leu Ile Gln Phe Lys Gln Gly Leu Asp Arg Met Thr Ala Gly Ser 
            180                 185                 190         


Asp Ser Leu Lys Gly Phe Lys Asp Ile Ile Thr Thr Lys Lys Phe Lys 
        195                 200                 205             


Lys Val Phe Pro Thr Leu Ser Leu Gly Leu Asp Lys Glu Val Arg Tyr 
    210                 215                 220                 


Ala Tyr Arg Gly Gly Phe Thr Trp Leu Asn Asp Arg Phe Lys Glu Lys 
225                 230                 235                 240 


Glu Ile Gly Glu Gly Met Val Phe Asp Val Asn Ser Leu Tyr Pro Ala 
                245                 250                 255     


Gln Met Tyr Ser Arg Leu Leu Pro Tyr Gly Glu Pro Ile Val Phe Glu 
            260                 265                 270         


Gly Lys Tyr Val Trp Asp Glu Asp Tyr Pro Leu His Ile Gln His Ile 
        275                 280                 285             


Arg Cys Glu Phe Glu Leu Lys Glu Gly Tyr Ile Pro Thr Ile Gln Ile 
    290                 295                 300                 


Lys Arg Ser Arg Phe Tyr Lys Gly Asn Glu Tyr Leu Lys Ser Ser Gly 
305                 310                 315                 320 


Gly Glu Ile Ala Asp Leu Trp Leu Ser Asn Val Asp Leu Glu Leu Met 
                325                 330                 335     


Lys Glu His Tyr Asp Leu Tyr Asn Val Glu Tyr Ile Ser Gly Leu Lys 
            340                 345                 350         


Phe Lys Ala Thr Thr Gly Leu Phe Lys Asp Phe Ile Asp Lys Trp Thr 
        355                 360                 365             


Tyr Ile Lys Thr Thr Ser Glu Gly Ala Ile Lys Gln Leu Ala Lys Leu 
    370                 375                 380                 


Met Leu Asn Ser Leu Tyr Gly Lys Phe Ala Ser Asn Pro Asp Val Thr 
385                 390                 395                 400 


Gly Lys Val Pro Tyr Leu Lys Glu Asn Gly Ala Leu Gly Phe Arg Leu 
                405                 410                 415     


Gly Glu Glu Glu Thr Lys Asp Pro Val Tyr Thr Pro Met Gly Val Phe 
            420                 425                 430         


Ile Thr Ala Trp Ala Arg Tyr Thr Thr Ile Thr Ala Ala Gln Ala Cys 
        435                 440                 445             


Tyr Asp Arg Ile Ile Tyr Cys Asp Thr Asp Ser Ile His Leu Thr Gly 
    450                 455                 460                 


Thr Glu Ile Pro Asp Val Ile Lys Asp Ile Val Asp Pro Lys Lys Leu 
465                 470                 475                 480 


Gly Tyr Trp Ala His Glu Ser Thr Phe Lys Arg Ala Lys Tyr Leu Arg 
                485                 490                 495     


Gln Lys Thr Tyr Ile Gln Asp Ile Tyr Met Lys Glu Val Asp Gly Lys 
            500                 505                 510         


Leu Val Glu Gly Ser Pro Asp Asp Tyr Thr Asp Ile Lys Phe Ser Val 
        515                 520                 525             


Lys Cys Ala Gly Met Thr Asp Lys Ile Lys Lys Glu Val Thr Phe Glu 
    530                 535                 540                 


Asn Phe Lys Val Gly Phe Ser Arg Lys Met Lys Pro Lys Pro Val Gln 
545                 550                 555                 560 


Val Pro Gly Gly Val Val Leu Val Asp Asp Thr Phe Thr Ile Lys Ser 
                565                 570                 575     


Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser 
            580                 585                 590         


Gly Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
        595                 600                 605             


<210>  7
<211>  1458
<212>  DNA
<213>  Escherichia coli

<400>  7
atgatgaacg atggcaaaca gcagagcacc ttcctgtttc atgattatga aaccttcggt       60

acccatccgg ccctggatcg tccggcgcag tttgcggcca ttcgcaccga tagcgaattc      120

aatgtgattg gcgaaccgga agtgttttat tgcaaaccgg ccgatgatta tctgccgcag      180

ccgggtgcgg tgctgattac cggtattacc ccgcaggaag cgcgcgcgaa aggtgaaaac      240

gaagcggcgt ttgccgcgcg cattcatagc ctgtttaccg tgccgaaaac ctgcattctg      300

ggctataaca atgtgcgctt cgatgatgaa gttacccgta atatctttta tcgtaacttt      360

tatgatccgt atgcgtggag ctggcagcat gataacagcc gttgggatct gctggatgtg      420

atgcgcgcgt gctatgcgct gcgcccggaa ggcattaatt ggccggaaaa cgatgatggc      480

ctgccgagct ttcgtctgga acatctgacc aaagccaacg gcattgaaca tagcaatgcc      540

catgatgcga tggccgatgt ttatgcgacc attgcgatgg cgaaactggt taaaacccgt      600

cagccgcgcc tgtttgatta tctgtttacc caccgtaaca aacacaaact gatggcgctg      660

attgatgttc cgcagatgaa accgctggtg catgtgagcg gcatgtttgg cgcctggcgc      720

ggcaacacca gctgggtggc cccgctggcc tggcacccgg aaaatcgtaa cgccgtgatt      780

atggttgatc tggccggtga tattagcccg ctgctggaac tggatagcga taccctgcgt      840

gaacgcctgt ataccgccaa aaccgatctg ggcgataatg ccgccgtgcc ggtgaaactg      900

gttcacatta acaaatgccc ggtgctggcc caggcgaaca ccctgcgccc ggaagatgcg      960

gatcgtctgg gtattaatcg ccagcattgt ctggataatc tgaaaatcct gcgtgaaaac     1020

ccgcaggtgc gtgaaaaagt ggtggcgatc ttcgcggaag cggaaccgtt caccccgagc     1080

gataacgtgg atgcgcagct gtataacggc ttctttagcg atgccgatcg cgcggcgatg     1140

aaaatcgttc tggaaaccga accgcgcaat ctgccggcgc tggatattac ctttgttgat     1200

aaacgtattg aaaaactgct gtttaattat cgtgcgcgca attttccggg taccctggat     1260

tatgccgaac agcagcgttg gctggaacat cgtcgtcagg ttttcacccc ggaatttctg     1320

cagggttatg cggatgaact gcagatgctg gttcagcagt atgccgatga taaagaaaaa     1380

gtggcgctgc tgaaagcgct gtggcagtat gcggaagaaa tcgtttctgg ctctggtcac     1440

catcatcatc accactaa                                                   1458


<210>  8
<211>  485
<212>  PRT
<213>  Escherichia coli

<400>  8

Met Met Asn Asp Gly Lys Gln Gln Ser Thr Phe Leu Phe His Asp Tyr 
1               5                   10                  15      


Glu Thr Phe Gly Thr His Pro Ala Leu Asp Arg Pro Ala Gln Phe Ala 
            20                  25                  30          


Ala Ile Arg Thr Asp Ser Glu Phe Asn Val Ile Gly Glu Pro Glu Val 
        35                  40                  45              


Phe Tyr Cys Lys Pro Ala Asp Asp Tyr Leu Pro Gln Pro Gly Ala Val 
    50                  55                  60                  


Leu Ile Thr Gly Ile Thr Pro Gln Glu Ala Arg Ala Lys Gly Glu Asn 
65                  70                  75                  80  


Glu Ala Ala Phe Ala Ala Arg Ile His Ser Leu Phe Thr Val Pro Lys 
                85                  90                  95      


Thr Cys Ile Leu Gly Tyr Asn Asn Val Arg Phe Asp Asp Glu Val Thr 
            100                 105                 110         


Arg Asn Ile Phe Tyr Arg Asn Phe Tyr Asp Pro Tyr Ala Trp Ser Trp 
        115                 120                 125             


Gln His Asp Asn Ser Arg Trp Asp Leu Leu Asp Val Met Arg Ala Cys 
    130                 135                 140                 


Tyr Ala Leu Arg Pro Glu Gly Ile Asn Trp Pro Glu Asn Asp Asp Gly 
145                 150                 155                 160 


Leu Pro Ser Phe Arg Leu Glu His Leu Thr Lys Ala Asn Gly Ile Glu 
                165                 170                 175     


His Ser Asn Ala His Asp Ala Met Ala Asp Val Tyr Ala Thr Ile Ala 
            180                 185                 190         


Met Ala Lys Leu Val Lys Thr Arg Gln Pro Arg Leu Phe Asp Tyr Leu 
        195                 200                 205             


Phe Thr His Arg Asn Lys His Lys Leu Met Ala Leu Ile Asp Val Pro 
    210                 215                 220                 


Gln Met Lys Pro Leu Val His Val Ser Gly Met Phe Gly Ala Trp Arg 
225                 230                 235                 240 


Gly Asn Thr Ser Trp Val Ala Pro Leu Ala Trp His Pro Glu Asn Arg 
                245                 250                 255     


Asn Ala Val Ile Met Val Asp Leu Ala Gly Asp Ile Ser Pro Leu Leu 
            260                 265                 270         


Glu Leu Asp Ser Asp Thr Leu Arg Glu Arg Leu Tyr Thr Ala Lys Thr 
        275                 280                 285             


Asp Leu Gly Asp Asn Ala Ala Val Pro Val Lys Leu Val His Ile Asn 
    290                 295                 300                 


Lys Cys Pro Val Leu Ala Gln Ala Asn Thr Leu Arg Pro Glu Asp Ala 
305                 310                 315                 320 


Asp Arg Leu Gly Ile Asn Arg Gln His Cys Leu Asp Asn Leu Lys Ile 
                325                 330                 335     


Leu Arg Glu Asn Pro Gln Val Arg Glu Lys Val Val Ala Ile Phe Ala 
            340                 345                 350         


Glu Ala Glu Pro Phe Thr Pro Ser Asp Asn Val Asp Ala Gln Leu Tyr 
        355                 360                 365             


Asn Gly Phe Phe Ser Asp Ala Asp Arg Ala Ala Met Lys Ile Val Leu 
    370                 375                 380                 


Glu Thr Glu Pro Arg Asn Leu Pro Ala Leu Asp Ile Thr Phe Val Asp 
385                 390                 395                 400 


Lys Arg Ile Glu Lys Leu Leu Phe Asn Tyr Arg Ala Arg Asn Phe Pro 
                405                 410                 415     


Gly Thr Leu Asp Tyr Ala Glu Gln Gln Arg Trp Leu Glu His Arg Arg 
            420                 425                 430         


Gln Val Phe Thr Pro Glu Phe Leu Gln Gly Tyr Ala Asp Glu Leu Gln 
        435                 440                 445             


Met Leu Val Gln Gln Tyr Ala Asp Asp Lys Glu Lys Val Ala Leu Leu 
    450                 455                 460                 


Lys Ala Leu Trp Gln Tyr Ala Glu Glu Ile Val Ser Gly Ser Gly His 
465                 470                 475                 480 


His His His His His 
                485 


<210>  9
<211>  804
<212>  DNA
<213>  Escherichia coli

<400>  9
atgaaatttg ttagcttcaa tatcaacggc ctgcgcgcgc gcccgcatca gctggaagcg       60

attgtggaaa aacatcagcc ggatgttatt ggtctgcagg aaaccaaagt tcacgatgat      120

atgtttccgc tggaagaagt ggcgaaactg ggctataacg tgttttatca tggccagaaa      180

ggtcattatg gcgtggccct gctgaccaaa gaaaccccga tcgcggttcg tcgtggtttt      240

ccgggtgatg atgaagaagc gcagcgtcgt attattatgg cggaaattcc gagcctgctg      300

ggcaatgtga ccgttattaa cggctatttt ccgcagggcg aaagccgtga tcatccgatt      360

aaatttccgg ccaaagcgca gttctatcag aacctgcaga actatctgga aaccgaactg      420

aaacgtgata atccggtgct gatcatgggc gatatgaaca ttagcccgac cgatctggat      480

attggcattg gcgaagaaaa ccgtaaacgc tggctgcgta ccggtaaatg cagctttctg      540

ccggaagaac gtgaatggat ggatcgcctg atgagctggg gcctggtgga tacctttcgt      600

catgcgaacc cgcagaccgc cgatcgcttt agctggtttg attatcgcag caaaggtttt      660

gatgataacc gtggcctgcg cattgatctg ctgctggcga gccagccgct ggcggaatgc      720

tgcgttgaaa ccggtattga ttatgaaatt cgcagcatgg aaaaaccgag cgatcacgcc      780

ccggtgtggg cgacctttcg ccgc                                             804


<210>  10
<211>  268
<212>  PRT
<213>  Escherichia coli

<400>  10

Met Lys Phe Val Ser Phe Asn Ile Asn Gly Leu Arg Ala Arg Pro His 
1               5                   10                  15      


Gln Leu Glu Ala Ile Val Glu Lys His Gln Pro Asp Val Ile Gly Leu 
            20                  25                  30          


Gln Glu Thr Lys Val His Asp Asp Met Phe Pro Leu Glu Glu Val Ala 
        35                  40                  45              


Lys Leu Gly Tyr Asn Val Phe Tyr His Gly Gln Lys Gly His Tyr Gly 
    50                  55                  60                  


Val Ala Leu Leu Thr Lys Glu Thr Pro Ile Ala Val Arg Arg Gly Phe 
65                  70                  75                  80  


Pro Gly Asp Asp Glu Glu Ala Gln Arg Arg Ile Ile Met Ala Glu Ile 
                85                  90                  95      


Pro Ser Leu Leu Gly Asn Val Thr Val Ile Asn Gly Tyr Phe Pro Gln 
            100                 105                 110         


Gly Glu Ser Arg Asp His Pro Ile Lys Phe Pro Ala Lys Ala Gln Phe 
        115                 120                 125             


Tyr Gln Asn Leu Gln Asn Tyr Leu Glu Thr Glu Leu Lys Arg Asp Asn 
    130                 135                 140                 


Pro Val Leu Ile Met Gly Asp Met Asn Ile Ser Pro Thr Asp Leu Asp 
145                 150                 155                 160 


Ile Gly Ile Gly Glu Glu Asn Arg Lys Arg Trp Leu Arg Thr Gly Lys 
                165                 170                 175     


Cys Ser Phe Leu Pro Glu Glu Arg Glu Trp Met Asp Arg Leu Met Ser 
            180                 185                 190         


Trp Gly Leu Val Asp Thr Phe Arg His Ala Asn Pro Gln Thr Ala Asp 
        195                 200                 205             


Arg Phe Ser Trp Phe Asp Tyr Arg Ser Lys Gly Phe Asp Asp Asn Arg 
    210                 215                 220                 


Gly Leu Arg Ile Asp Leu Leu Leu Ala Ser Gln Pro Leu Ala Glu Cys 
225                 230                 235                 240 


Cys Val Glu Thr Gly Ile Asp Tyr Glu Ile Arg Ser Met Glu Lys Pro 
                245                 250                 255     


Ser Asp His Ala Pro Val Trp Ala Thr Phe Arg Arg 
            260                 265             


<210>  11
<211>  2000
<212>  DNA
<213>  Thermus thermophilus

<400>  11
atgcgtgatc gtgtccgctg gcgtgttctg tccctgccgc cgctggctca atggcgtgaa       60

gttatggctg ccctggaagt gggtccggaa gcggccctgg catattggca ccgcggtttt      120

cgtcgcaaag aagatctgga cccgccgctg gccctgctgc cgctgaaagg cctgcgcgaa      180

gcagctgcgc tgctggaaga agccctgcgt cagggtaaac gtattcgcgt tcatggcgat      240

tatgacgccg atggcctgac cggtaccgca attctggtcc gtggtctggc ggcactgggt      300

gccgatgtgc atccgtttat tccgcaccgc ctggaagaag gctacggtgt gctgatggaa      360

cgtgttccgg aacacctgga agcgagcgac ctgttcctga cggtggattg cggtatcacc      420

aaccatgccg aactgcgtga actgctggaa aatggcgttg aagtcattgt gaccgatcat      480

cacaccccgg gtaaaacccc gtctccgggc ctggtggttc acccggcgct gacgccggat      540

ctgaaagaaa aaccgaccgg cgctggtgtc gtgtttctgc tgctgtgggc actgcacgaa      600

cgtctgggtc tgccgccgcc gctggaatat gccgacctgg ctgccgttgg taccattgcc      660

gatgttgccc cgctgtgggg ttggaaccgt gcactggtga aagaaggcct ggcacgtatt      720

ccggctagct cttgggttgg tctgcgtctg ctggccgaag cagtcggcta caccggtaaa      780

gcggttgaag tcgccttccg tattgcaccg cgcatcaacg ccgcatcacg cctgggtgaa      840

gcagaaaaag ctctgcgtct gctgctgacg gatgacgctg cggaagctca ggcgctggtt      900

ggcgaactgc accgcctgaa tgctcgtcgc cagaccctgg aagaagcgat gctgcgtaaa      960

ctgctgccgc aagcggaccc ggaagccaaa gcaatcgtgc tgctggatcc ggaaggccat     1020

ccgggtgtta tgggcattgt cgcttcacgc atcctggaag cgacgctgcg tccggtcttt     1080

ctggtggcgc agggtaaagg taccgtgcgc agcctggcac cgatttctgc cgttgaagcc     1140

ctgcgtagcg ccgaagacct gctgctgcgt tatggcggtc acaaagaagc cgcaggcttt     1200

gctatggatg aagcgctgtt tccggcattc aaagctcgcg ttgaagccta cgctgcccgt     1260

ttcccggacc cggttcgtga agtcgcactg ctggatctgc tgccggaacc gggtctgctg     1320

ccgcaggtgt ttcgtgaact ggcgctgctg gaaccgtatg gcgaaggtaa tccggaaccg     1380

ctgtttctgc tgtttggtgc accggaagaa gcacgtcgcc tgggtgaagg tcgtcacctg     1440

gcattccgcc tgaaaggtgt gcgtgttctg gcttggaaac agggtgatct ggccctgccg     1500

ccggaagttg aagtggcagg tctgctgtcc gaaaacgcat ggaatggcca tctggcctat     1560

gaagtgcaag cagttgatct gcgtaaaccg gaagcgctgg aaggcggtat tgccccgttt     1620

gcatatccgc tgccgctgct ggaagctctg gcccgtgccc gcctgggcga aggtgtttat     1680

gtcccggaag acaacccgga aggtctggat tacgcacgca aagctggctt ccgtctgctg     1740

ccgccggaag aagcgggtct gtggctgggt ctgccgccgc gcccggtgct gggtcgtcgc     1800

gtggaagttg cactgggccg tgaagcacgt gctcgcctga gtgcaccgcc ggttctgcat     1860

accccggaag ctcgcctgaa agcgctggtg caccgtcgcc tgctgtttgc ctatgaacgt     1920

cgccatccgg gtctgttctc cgaagcgctg ctggcctact gggaagtcaa tcgtgttcag     1980

gaaccggcgg gtagtcctaa                                                 2000


<210>  12
<211>  666
<212>  PRT
<213>  Thermus thermophilus

<400>  12

Met Arg Asp Arg Val Arg Trp Arg Val Leu Ser Leu Pro Pro Leu Ala 
1               5                   10                  15      


Gln Trp Arg Glu Val Met Ala Ala Leu Glu Val Gly Pro Glu Ala Ala 
            20                  25                  30          


Leu Ala Tyr Trp His Arg Gly Phe Arg Arg Lys Glu Asp Leu Asp Pro 
        35                  40                  45              


Pro Leu Ala Leu Leu Pro Leu Lys Gly Leu Arg Glu Ala Ala Ala Leu 
    50                  55                  60                  


Leu Glu Glu Ala Leu Arg Gln Gly Lys Arg Ile Arg Val His Gly Asp 
65                  70                  75                  80  


Tyr Asp Ala Asp Gly Leu Thr Gly Thr Ala Ile Leu Val Arg Gly Leu 
                85                  90                  95      


Ala Ala Leu Gly Ala Asp Val His Pro Phe Ile Pro His Arg Leu Glu 
            100                 105                 110         


Glu Gly Tyr Gly Val Leu Met Glu Arg Val Pro Glu His Leu Glu Ala 
        115                 120                 125             


Ser Asp Leu Phe Leu Thr Val Asp Cys Gly Ile Thr Asn His Ala Glu 
    130                 135                 140                 


Leu Arg Glu Leu Leu Glu Asn Gly Val Glu Val Ile Val Thr Asp His 
145                 150                 155                 160 


His Thr Pro Gly Lys Thr Pro Ser Pro Gly Leu Val Val His Pro Ala 
                165                 170                 175     


Leu Thr Pro Asp Leu Lys Glu Lys Pro Thr Gly Ala Gly Val Val Phe 
            180                 185                 190         


Leu Leu Leu Trp Ala Leu His Glu Arg Leu Gly Leu Pro Pro Pro Leu 
        195                 200                 205             


Glu Tyr Ala Asp Leu Ala Ala Val Gly Thr Ile Ala Asp Val Ala Pro 
    210                 215                 220                 


Leu Trp Gly Trp Asn Arg Ala Leu Val Lys Glu Gly Leu Ala Arg Ile 
225                 230                 235                 240 


Pro Ala Ser Ser Trp Val Gly Leu Arg Leu Leu Ala Glu Ala Val Gly 
                245                 250                 255     


Tyr Thr Gly Lys Ala Val Glu Val Ala Phe Arg Ile Ala Pro Arg Ile 
            260                 265                 270         


Asn Ala Ala Ser Arg Leu Gly Glu Ala Glu Lys Ala Leu Arg Leu Leu 
        275                 280                 285             


Leu Thr Asp Asp Ala Ala Glu Ala Gln Ala Leu Val Gly Glu Leu His 
    290                 295                 300                 


Arg Leu Asn Ala Arg Arg Gln Thr Leu Glu Glu Ala Met Leu Arg Lys 
305                 310                 315                 320 


Leu Leu Pro Gln Ala Asp Pro Glu Ala Lys Ala Ile Val Leu Leu Asp 
                325                 330                 335     


Pro Glu Gly His Pro Gly Val Met Gly Ile Val Ala Ser Arg Ile Leu 
            340                 345                 350         


Glu Ala Thr Leu Arg Pro Val Phe Leu Val Ala Gln Gly Lys Gly Thr 
        355                 360                 365             


Val Arg Ser Leu Ala Pro Ile Ser Ala Val Glu Ala Leu Arg Ser Ala 
    370                 375                 380                 


Glu Asp Leu Leu Leu Arg Tyr Gly Gly His Lys Glu Ala Ala Gly Phe 
385                 390                 395                 400 


Ala Met Asp Glu Ala Leu Phe Pro Ala Phe Lys Ala Arg Val Glu Ala 
                405                 410                 415     


Tyr Ala Ala Arg Phe Pro Asp Pro Val Arg Glu Val Ala Leu Leu Asp 
            420                 425                 430         


Leu Leu Pro Glu Pro Gly Leu Leu Pro Gln Val Phe Arg Glu Leu Ala 
        435                 440                 445             


Leu Leu Glu Pro Tyr Gly Glu Gly Asn Pro Glu Pro Leu Phe Leu Leu 
    450                 455                 460                 


Phe Gly Ala Pro Glu Glu Ala Arg Arg Leu Gly Glu Gly Arg His Leu 
465                 470                 475                 480 


Ala Phe Arg Leu Lys Gly Val Arg Val Leu Ala Trp Lys Gln Gly Asp 
                485                 490                 495     


Leu Ala Leu Pro Pro Glu Val Glu Val Ala Gly Leu Leu Ser Glu Asn 
            500                 505                 510         


Ala Trp Asn Gly His Leu Ala Tyr Glu Val Gln Ala Val Asp Leu Arg 
        515                 520                 525             


Lys Pro Glu Ala Leu Glu Gly Gly Ile Ala Pro Phe Ala Tyr Pro Leu 
    530                 535                 540                 


Pro Leu Leu Glu Ala Leu Ala Arg Ala Arg Leu Gly Glu Gly Val Tyr 
545                 550                 555                 560 


Val Pro Glu Asp Asn Pro Glu Gly Leu Asp Tyr Ala Arg Lys Ala Gly 
                565                 570                 575     


Phe Arg Leu Leu Pro Pro Glu Glu Ala Gly Leu Trp Leu Gly Leu Pro 
            580                 585                 590         


Pro Arg Pro Val Leu Gly Arg Arg Val Glu Val Ala Leu Gly Arg Glu 
        595                 600                 605             


Ala Arg Ala Arg Leu Ser Ala Pro Pro Val Leu His Thr Pro Glu Ala 
    610                 615                 620                 


Arg Leu Lys Ala Leu Val His Arg Arg Leu Leu Phe Ala Tyr Glu Arg 
625                 630                 635                 640 


Arg His Pro Gly Leu Phe Ser Glu Ala Leu Leu Ala Tyr Trp Glu Val 
                645                 650                 655     


Asn Arg Val Gln Glu Pro Ala Gly Ser Pro 
            660                 665     


<210>  13
<211>  681
<212>  DNA
<213>  Bacteriophage lambda

<400>  13
atgacaccgg acattatcct gcagcgtacc gggatcgatg tgagagctgt cgaacagggg       60

gatgatgcgt ggcacaaatt acggctcggc gtcatcaccg cttcagaagt tcacaacgtg      120

atagcaaaac cccgctccgg aaagaagtgg cctgacatga aaatgtccta cttccacacc      180

ctgcttgctg aggtttgcac cggtgtggct ccggaagtta acgctaaagc actggcctgg      240

ggaaaacagt acgagaacga cgccagaacc ctgtttgaat tcacttccgg cgtgaatgtt      300

actgaatccc cgatcatcta tcgcgacgaa agtatgcgta ccgcctgctc tcccgatggt      360

ttatgcagtg acggcaacgg ccttgaactg aaatgcccgt ttacctcccg ggatttcatg      420

aagttccggc tcggtggttt cgaggccata aagtcagctt acatggccca ggtgcagtac      480

agcatgtggg tgacgcgaaa aaatgcctgg tactttgcca actatgaccc gcgtatgaag      540

cgtgaaggcc tgcattatgt cgtgattgag cgggatgaaa agtacatggc gagttttgac      600

gagatcgtgc cggagttcat cgaaaaaatg gacgaggcac tggctgaaat tggttttgta      660

tttggggagc aatggcgata a                                                681


<210>  14
<211>  226
<212>  PRT
<213>  Bacteriophage lambda

<400>  14

Met Thr Pro Asp Ile Ile Leu Gln Arg Thr Gly Ile Asp Val Arg Ala 
1               5                   10                  15      


Val Glu Gln Gly Asp Asp Ala Trp His Lys Leu Arg Leu Gly Val Ile 
            20                  25                  30          


Thr Ala Ser Glu Val His Asn Val Ile Ala Lys Pro Arg Ser Gly Lys 
        35                  40                  45              


Lys Trp Pro Asp Met Lys Met Ser Tyr Phe His Thr Leu Leu Ala Glu 
    50                  55                  60                  


Val Cys Thr Gly Val Ala Pro Glu Val Asn Ala Lys Ala Leu Ala Trp 
65                  70                  75                  80  


Gly Lys Gln Tyr Glu Asn Asp Ala Arg Thr Leu Phe Glu Phe Thr Ser 
                85                  90                  95      


Gly Val Asn Val Thr Glu Ser Pro Ile Ile Tyr Arg Asp Glu Ser Met 
            100                 105                 110         


Arg Thr Ala Cys Ser Pro Asp Gly Leu Cys Ser Asp Gly Asn Gly Leu 
        115                 120                 125             


Glu Leu Lys Cys Pro Phe Thr Ser Arg Asp Phe Met Lys Phe Arg Leu 
    130                 135                 140                 


Gly Gly Phe Glu Ala Ile Lys Ser Ala Tyr Met Ala Gln Val Gln Tyr 
145                 150                 155                 160 


Ser Met Trp Val Thr Arg Lys Asn Ala Trp Tyr Phe Ala Asn Tyr Asp 
                165                 170                 175     


Pro Arg Met Lys Arg Glu Gly Leu His Tyr Val Val Ile Glu Arg Asp 
            180                 185                 190         


Glu Lys Tyr Met Ala Ser Phe Asp Glu Ile Val Pro Glu Phe Ile Glu 
        195                 200                 205             


Lys Met Asp Glu Ala Leu Ala Glu Ile Gly Phe Val Phe Gly Glu Gln 
    210                 215                 220                 


Trp Arg 
225     


<210>  15
<211>  184
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  15

Gly Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu 
1               5                   10                  15      


Thr Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp 
            20                  25                  30          


Arg Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr 
        35                  40                  45              


Ile Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu 
    50                  55                  60                  


Gly Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe 
65                  70                  75                  80  


Ser Tyr Thr Thr Pro Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Ala 
                85                  90                  95      


Pro Pro Phe Gly Leu Asn Ser Val Ile Thr Pro Asn Leu Phe Pro Gly 
            100                 105                 110         


Val Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val 
        115                 120                 125             


Ala Thr Phe Ser Val Asp Val Ser Gly Pro Ala Gly Gly Val Ala Val 
    130                 135                 140                 


Ser Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu 
145                 150                 155                 160 


Arg Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr 
                165                 170                 175     


Tyr Gly Glu Pro Trp Asn Met Asn 
            180                 


<210>  16
<211>  184
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  16

Gly Leu Asp Asn Glu Leu Ser Leu Val Asp Gly Gln Asp Arg Thr Leu 
1               5                   10                  15      


Thr Val Gln Gln Trp Asp Thr Phe Leu Asn Gly Val Phe Pro Leu Asp 
            20                  25                  30          


Arg Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Lys Tyr 
        35                  40                  45              


Ile Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu 
    50                  55                  60                  


Gly Tyr Gln Ile Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe 
65                  70                  75                  80  


Ser Tyr Thr Thr Pro Asn Ile Leu Ile Asp Asp Gly Asp Ile Thr Gly 
                85                  90                  95      


Pro Pro Phe Gly Leu Glu Ser Val Ile Thr Pro Asn Leu Phe Pro Gly 
            100                 105                 110         


Val Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val 
        115                 120                 125             


Ala Thr Phe Ser Val Asp Val Ser Gly Pro Ala Gly Gly Val Ala Val 
    130                 135                 140                 


Ser Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu 
145                 150                 155                 160 


Arg Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr 
                165                 170                 175     


Tyr Gly Glu Pro Trp Asn Met Asn 
            180                 


<210>  17
<211>  183
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  17

Val Asp Asn Gln Leu Ser Val Val Asp Gly Gln Gly Arg Thr Leu Thr 
1               5                   10                  15      


Val Gln Gln Ala Glu Thr Phe Leu Asn Gly Val Phe Pro Leu Asp Arg 
            20                  25                  30          


Asn Arg Leu Thr Arg Glu Trp Phe His Ser Gly Arg Ala Thr Tyr His 
        35                  40                  45              


Val Ala Gly Pro Gly Ala Asp Glu Phe Glu Gly Thr Leu Glu Leu Gly 
    50                  55                  60                  


Tyr Gln Val Gly Phe Pro Trp Ser Leu Gly Val Gly Ile Asn Phe Ser 
65                  70                  75                  80  


Tyr Thr Thr Pro Asn Ile Leu Ile Asp Gly Gly Asp Ile Thr Gln Pro 
                85                  90                  95      


Pro Phe Gly Leu Asp Thr Ile Ile Thr Pro Asn Leu Phe Pro Gly Val 
            100                 105                 110         


Ser Ile Ser Ala Asp Leu Gly Asn Gly Pro Gly Ile Gln Glu Val Ala 
        115                 120                 125             


Thr Phe Ser Val Asp Val Lys Gly Ala Lys Gly Ala Val Ala Val Ser 
    130                 135                 140                 


Asn Ala His Gly Thr Val Thr Gly Ala Ala Gly Gly Val Leu Leu Arg 
145                 150                 155                 160 


Pro Phe Ala Arg Leu Ile Ala Ser Thr Gly Asp Ser Val Thr Thr Tyr 
                165                 170                 175     


Gly Glu Pro Trp Asn Met Asn 
            180             


<210>  18
<211>  70
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence used in the examples

<400>  18
tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt       60

tttttttttt                                                              70


<210>  19
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the examples

<400>  19
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa       60

aaaaaaaaaa                                                              70


<210>  20
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the examples

<400>  20
cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc       60

cccccccccc                                                              70


<210>  21
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the examples

<400>  21
tgtgttctat gtcttattct tacttcgtta ttcttgtctc tattctgttt atgtttcttg       60

tttgttagca                                                              70


<210>  22
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the examples

<400>  22
tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt       60

tttttttttt ggctacgacc tgcatgagaa tgc                                    93


<210>  23
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the examples

<400>  23
ctcacctatc cttccactca ccccccaaaa aaccccccaa aaaacccccc aaaaaagcat       60

tctcatgcag gtcgtagcc                                                    79


<210>  24
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the examples

<400>  24
aacccccaaa aacccccaaa aacccccaaa aacccccaaa aacccccaaa aacccccaaa       60

aacccccaaa aacccccata gagacaagaa taacgaagta                            100


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the examples

<400>  25
tacttcgtta ttcttgtctc tat                                               23


<210>  26
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the examples

<400>  26
cccccccccc cccccacccc cccccccccc ccccctattc tgtttatgtt tcttgtttgt       60

tagcc                                                                   65


<210>  27
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the examples

<400>  27
ggctaacaaa caagaaacat aaacagaata g                                      31


<210>  28
<211>  65
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence used in the examples

<400>  28
cccccccccc cccccacccc cccccccccc ccccctattc tgtttatgtt tcttgtttgt       60

tagcc                                                                   65


<210>  29
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the examples


<220>
<221>  misc_feature
<222>  (51)..(54)
<223>  n = abasic nucleotide

<400>  29
tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt nnnnggttgt       60

ttctgttggt gctgatattg c                                                 81


<210>  30
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in examples

<400>  30
gttagaccaa accatgaaac caacata                                           27


<210>  31
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in examples

<400>  31
gaccgcctcc aaacaattta gaca                                              24


<210>  32
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the examples

<400>  32
ggcaatctct ttctgattgt ccag                                              24


<210>  33
<211>  885
<212>  DNA
<213>  Artificial sequence

<220>
<223>   sequence for one subunit of alpha-HL-Q

<400>  33
atggcagatt ctgatattaa tattaaaacc ggtactacag atattggaag caatactaca       60

gtaaaaacag gtgatttagt cacttatgat aaagaaaatg gcatgcacaa aaaagtattt      120

tatagtttta tcgatgataa aaatcacaat aaaaaactgc tagttattag aacaaaaggt      180

accattgctg gtcaatatag agtttatagc gaagaaggtg ctaacaaaag tggtttagcc      240

tggccttcag cctttaaggt acagttgcaa ctacctgata atgaagtagc tcaaatatct      300

gattactatc caagaaattc gattgataca aaagagtata tgagtacttt aacttatgga      360

ttcaacggta atgttactgg tgatgataca ggaaaaattg gcggccttat tggtgcacaa      420

gtttcgattg gtcatacact gaaatatgtt caacctgatt tcaaaacaat tttagagagc      480

ccaactgata aaaaagtagg ctggaaagtg atatttaaca atatggtgaa tcaaaattgg      540

ggaccatacg atcgagattc ttggaacccg gtatatggca atcaactttt catgaaaact      600

agaaatggtt ctatgaaagc agcagataac ttccttgatc ctaacaaagc aagttctcta      660

ttatcttcag ggttttcacc agacttcgct acagttatta ctatggatag aaaagcatcc      720

aaacaacaaa caaatataga tgtaatatac gaacgagttc gtgatgatta ccaattgcat      780

tggacttcaa caaattggaa aggtaccaat actaaagata aatggacaga tcgttcttca      840

gaaagatata aaatcgattg ggaaaaagaa gaaatgacaa attaa                      885


<210>  34
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protein sequence for one subunit of alpha-HL-Q

<400>  34

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Gln Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  35
<211>  957
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotide sequence of one subunit of 
       alpha-HL-E287C-QC-D5FLAG

<400>  35
atggcagatt ctgatattaa tattaaaacc ggtactacag atattggaag caatactaca       60

gtaaaaacag gtgatttagt cacttatgat aaagaaaatg gcatgcacaa aaaagtattt      120

tatagtttta tcgatgataa aaatcacaat aaaaaactgc tagttattag aacaaaaggt      180

accattgctg gtcaatatag agtttatagc gaagaaggtg ctaacaaaag tggtttagcc      240

tggccttcag cctttaaggt acagttgcaa ctacctgata atgaagtagc tcaaatatct      300

gattactatc caagaaattc gattgataca aaagagtata tgagtacttt aacttatgga      360

ttcaacggta atgttactgg tgatgataca ggaaaaattg gcggctgtat tggtgcacaa      420

gtttcgattg gtcatacact gaaatatgtt caacctgatt tcaaaacaat tttagagagc      480

ccaactgata aaaaagtagg ctggaaagtg atatttaaca atatggtgaa tcaaaattgg      540

ggaccatacg atcgagattc ttggaacccg gtatatggca atcaactttt catgaaaact      600

agaaatggtt ctatgaaagc agcagataac ttccttgatc ctaacaaagc aagttctcta      660

ttatcttcag ggttttcacc agacttcgct acagttatta ctatggatag aaaagcatcc      720

aaacaacaaa caaatataga tgtaatatac gaacgagttc gtgatgatta ccaattgcat      780

tggacttcaa caaattggaa aggtaccaat actaaagata aatggacaga tcgttcttca      840

gaaagatata aaatcgattg gtgcaaagaa gaaatgacaa atgatgatga tgatgatagc      900

ggcagcggtg attataaaga tgatgatgat aaagcgcacc atcatcatca ccactaa         957


<210>  36
<211>  317
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of one subunit of alpha-HL-E287C-QC-D5FLAG

<400>  36

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Cys Ile Gly Ala Gln Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Cys Lys 
        275                 280                 285             


Glu Glu Met Thr Asn Asp Asp Asp Asp Asp Ser Gly Ser Gly Asp Tyr 
    290                 295                 300                 


Lys Asp Asp Asp Asp Lys Ala His His His His His His 
305                 310                 315         


<210>  37
<211>  885
<212>  DNA
<213>  Artificial sequence

<220>
<223>  polynucleotide sequence of one subunit of 
       alpha-hemolysin-E111N/K147N

<400>  37
atggcagatt ctgatattaa tattaaaacc ggtactacag atattggaag caatactaca       60

gtaaaaacag gtgatttagt cacttatgat aaagaaaatg gcatgcacaa aaaagtattt      120

tatagtttta tcgatgataa aaatcacaat aaaaaactgc tagttattag aacaaaaggt      180

accattgctg gtcaatatag agtttatagc gaagaaggtg ctaacaaaag tggtttagcc      240

tggccttcag cctttaaggt acagttgcaa ctacctgata atgaagtagc tcaaatatct      300

gattactatc caagaaattc gattgataca aaaaactata tgagtacttt aacttatgga      360

ttcaacggta atgttactgg tgatgataca ggaaaaattg gcggccttat tggtgcaaat      420

gtttcgattg gtcatacact gaactatgtt caacctgatt tcaaaacaat tttagagagc      480

ccaactgata aaaaagtagg ctggaaagtg atatttaaca atatggtgaa tcaaaattgg      540

ggaccatacg atcgagattc ttggaacccg gtatatggca atcaactttt catgaaaact      600

agaaatggtt ctatgaaagc agcagataac ttccttgatc ctaacaaagc aagttctcta      660

ttatcttcag ggttttcacc agacttcgct acagttatta ctatggatag aaaagcatcc      720

aaacaacaaa caaatataga tgtaatatac gaacgagttc gtgatgatta ccaattgcat      780

tggacttcaa caaattggaa aggtaccaat actaaagata aatggacaga tcgttcttca      840

gaaagatata aaatcgattg ggaaaaagaa gaaatgacaa attaa                      885


<210>  38
<211>  293
<212>  PRT
<213>  artificial sequence

<220>
<223>  amino acid sequence of one subunit of alpha-hemolysin-E111N/K147N

<400>  38

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Asn Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Asn Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  39
<211>  518
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the examples

<400>  39
tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt ggttgtttct       60

gttggtgctg atattgcgct ccactaaagg gccgattgac ccggtggtac cttggttgtt      120

tctgttggtg ctgatattgc ttttgatgcc gaccctaaat tttttgcctg tttggttcgc      180

tttgagtctt cttcggttcc gactaccctc ccgactgcct atgatgttta tcctttggat      240

ggtcgccatg atggtggtta ttataccgtc aaggactgtg tgactattga cgtccttccc      300

cgtacgccgg gcaataatgt ttatgttggt ttcatggttt ggtctaactt taccgctact      360

aaatgccgcg gattggtttc gctgaatcag gttattaaag agattatttg tctccagcca      420

cttaagtgag gtgatttatg tttggtgcta ttgctggcgg tattgcttct gctcttgctg      480

gtggcgccat gtctaaattg tttggaggcg gtcgagct                              518


<210>  40
<211>  530
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the example

<400>  40
tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt ggttgtttct       60

gttggtgctg atattgcgct ccactaaagg gccgattgac gctccactaa agggccgatt      120

gacccggttg tttctgttgg tgctgatatt gcttttgatg ccgaccctaa attttttgcc      180

tgtttggttc gctttgagtc ttcttcggtt ccgactaccc tcccgactgc ctatgatgtt      240

tatcctttgg atggtcgcca tgatggtggt tattataccg tcaaggactg tgtgactatt      300

gacgtccttc cccgtacgcc gggcaataat gtttatgttg gtttcatggt ttggtctaac      360

tttaccgcta ctaaatgccg cggattggtt tcgctgaatc aggttattaa agagattatt      420

tgtctccagc cacttaagtg aggtgattta tgtttggtgc tattgctggc ggtattgctt      480

ctgctcttgc tggtggcgcc atgtctaaat tgtttggagg cggtcgagct                 530


<210>  41
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence used in the examples

<400>  41
agcgactaac aaacacaatc tgatggcttt tttttttttt tttttttttt ttttttt          57


