                         SEQUENCE LISTING

<110>  Sandoz AG
 
<120>  Transcription Terminator Sequences

<130>  r61689

<150>  EP11168637.4
       2011-06-03  

<160>  23    

<170>  PatentIn version 3.3

<210>  1
<211>  5422
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pET30a Vector Nucleotide Sequence

<400>  1
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa     60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt    120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt    180

cgacggagct cgaattcgga tccgatatca gccatggcct tgtcgtcgtc gtcggtaccc    240

agatctgggc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc tttcatacca    300

gaaccgcgtg gcaccagacc agaagaatga tgatgatgat ggtgcatatg tatatctcct    360

tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta    420

tagtgagtcg tattaatttc gcgggatcga gatcgatctc gatcctctac gccggacgca    480

tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca    540

ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta    600

tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc    660

ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt    720

cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac ctttcgcggt    780

atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg    840

ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac    900

caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg    960

aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc   1020

gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct   1080

cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa   1140

gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac   1200

tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg   1260

ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac   1320

ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta   1380

gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc   1440

actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt   1500

tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc   1560

aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt   1620

gcggacatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg   1680

ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg   1740

caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa   1800

agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca   1860

ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat   1920

taatgtaagt tagctcactc attaggcacc gggatctcga ccgatgccct tgagagcctt   1980

caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac   2040

tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg   2100

cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat   2160

cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa   2220

gcaggccatt atcgccggca tggcggcccc acgggtgcgc atgatcgtgc tcctgtcgtt   2280

gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg   2340

cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat   2400

ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat   2460

tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt   2520

attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac   2580

cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg   2640

tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc   2700

ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat   2760

cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc   2820

agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg   2880

tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg   2940

tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg   3000

gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac   3060

tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt gaaataccgc   3120

acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact   3180

cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac   3240

ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa   3300

aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg   3360

acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa   3420

gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc   3480

ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac   3540

gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac   3600

cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg   3660

taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt   3720

atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga   3780

cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct   3840

cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga   3900

ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg   3960

ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gaacaataaa actgtctgct   4020

tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctct   4080

aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat   4140

aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag   4200

ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga   4260

ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct   4320

gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa   4380

gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg   4440

cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag   4500

gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat   4560

ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat   4620

tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta   4680

ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc   4740

ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat   4800

ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc   4860

taagaattaa ttcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg   4920

ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt gtaaacgtta atattttgtt   4980

aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg   5040

caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg   5100

gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta   5160

tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg   5220

ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa   5280

gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct   5340

ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct   5400

acagggcgcg tcccattcgc ca                                            5422


<210>  2
<211>  5422
<212>  DNA
<213>  Artificial

<220>
<223>  pET30a Vector Reverse Complementary Nucleotide Sequence

<400>  2
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg     60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc    120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg    180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc    240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt    300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc    360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta    420

acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt    480

tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta    540

tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat    600

tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa    660

actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc    720

gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga    780

aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc    840

agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac    900

cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac    960

aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat   1020

tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag   1080

tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca   1140

taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac   1200

ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg   1260

tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca   1320

tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac   1380

cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa   1440

cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga   1500

gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg   1560

gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc   1620

agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag   1680

aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc   1740

agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg   1800

cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac   1860

accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga   1920

aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt   1980

ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag   2040

cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg   2100

gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta   2160

tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc   2220

agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg   2280

tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta   2340

caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg   2400

ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct   2460

gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag   2520

gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc   2580

gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag   2640

aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt   2700

ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa   2760

acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg   2820

ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg   2880

tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc   2940

tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta   3000

cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca   3060

gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc   3120

ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc   3180

catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa   3240

ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc   3300

gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac   3360

gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca   3420

ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta   3480

atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa   3540

cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat   3600

tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca   3660

ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa   3720

aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt   3780

atcccactac cgagatgtcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg   3840

cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca   3900

gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta   3960

tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg   4020

agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat   4080

gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct   4140

ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg   4200

catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat   4260

tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc   4320

tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca   4380

gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg   4440

ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt   4500

tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg   4560

catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct   4620

cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga   4680

tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg   4740

ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc   4800

ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg   4860

cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg   4920

gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatcga tctcgatccc   4980

gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa   5040

ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat catcattctt   5100

ctggtctggt gccacgcggt tctggtatga aagaaaccgc tgctgctaaa ttcgaacgcc   5160

agcacatgga cagcccagat ctgggtaccg acgacgacga caaggccatg gctgatatcg   5220

gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac   5280

cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct   5340

gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg   5400

aaaggaggaa ctatatccgg at                                            5422


<210>  3
<211>  73
<212>  DNA
<213>  Bacteriophage T7

<400>  3
aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag     60

gaggaactat atg                                                        73


<210>  4
<211>  43
<212>  RNA
<213>  Artificial

<220>
<223>  optimized terminator sequence

<400>  4
aaccccgcgg ggccucuucg ggggucucgc gggguuuuuu gcu                       43


<210>  5
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  SOD primer

<400>  5
gtcgtcggat ccttactatt gggcgatccc                                      30


<210>  6
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  SOD primer

<400>  6
gtcgtctcta gaaataattt tgtttaac                                        28


<210>  7
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  T7 deletion primer1

<400>  7
ttagcagccg gatctcagtg gtgg                                            24


<210>  8
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  T7 deletion primer2

<400>  8
ggaggaacta tatccggatt ggcg                                            24


<210>  9
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  class II terminator

<400>  9
tgtgtcccta tctgttacag tctcct                                          26


<210>  10
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  class II terminator

<400>  10
atgcttgcca tctgttttct tgcaag                                          26


<210>  11
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  class II terminator

<400>  11
atccatgata tctgttagtt tttttc                                          26


<210>  12
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  class II terminator

<400>  12
atccatgata tctgttctcg agttttttt                                       29


<210>  13
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  class II terminator

<400>  13
tttcgtttta tctgttgttt gtcgtg                                          26


<210>  14
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  class II terminator

<400>  14
tagttttgta tctgttttgc agcagc                                          26


<210>  15
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  class II terminator

<400>  15
ttcgaacctc tctgtttact gataag                                          26


<210>  16
<211>  46
<212>  DNA
<213>  vesicular stomatitis virus

<400>  16
tcgacctagc atatccatga tatctgttag tttttttcct gaaaga                    46


<210>  17
<211>  63
<212>  DNA
<213>  Bacteriophage T3

<400>  17
tcgacctagc ataaacccct tgggttccct ctttaggagt ctgaggggtt ttttgctgaa     60

aga                                                                   63


<210>  18
<211>  61
<212>  DNA
<213>  Bacteriophage T7

<400>  18
tcgacctagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag     60

a                                                                     61


<210>  19
<211>  61
<212>  DNA
<213>  Artificial

<220>
<223>  Optimized Bacteriophage T7 terminator

<400>  19
tcgacctagc ataaccccgc ggggcctctt cgggggtctc gcggggtttt ttgctgaaag     60

a                                                                     61


<210>  20
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  terminator insert

<400>  20
agctttgtgt ccctatctgt tacagtctcc tgc                                  33


<210>  21
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  terminator insert

<400>  21
ggccgcagga gactgtaaca gatagggaca caa                                  33


<210>  22
<211>  69
<212>  DNA
<213>  Artificial

<220>
<223>  terminator insert

<400>  22
ggccgcagcg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt     60

tttatttga                                                             69


<210>  23
<211>  69
<212>  DNA
<213>  Artificial

<220>
<223>  terminator insert

<400>  23
agcttcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt     60

tgtcgctgc                                                             69


