                               SEQUENCE LISTING

<110> SIEMENS HEALTHCARE DIAGNOSTICS, INC.
      SPENCER LIN
      QIMU LIAO
      BRUCE CAMPBELL
 
<120> ANTIBODIES TO 25-HYDROXYVITAMIN D2 AND D3 AND USES THEREOF

<130> SMSD-0099

<140>
<141>

<150> 61/488,630
<151> 2011-05-20

<160> 36    

<170> PatentIn version 3.5

<210> 1
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 1
ttttggggcc aaggcaccac tctcacagtc tcctcg                                 36


<210> 2
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 2
tacaccatga actgggtgaa gcagagccat ggaaagaac                              39


<210> 3
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 3
tttactatct ataatcagaa g                                                 21


<210> 4
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 4
ataagagcgc attacgacgg gagagtt                                           27


<210> 5
<211> 90
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 5
gtgcagctgc tcgagtctgg acctgagctg gtgaagcctg gagcttcaat gaagatatcc       60

tgcaaggctt ctggttactc attcactgac                                        90


<210> 6
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 6
cttgagtgga ttggacttat taatccttac aatggt                                 36


<210> 7
<211> 99
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 7
ttcaagggca aggccacatt aactgtagac aagtcatcca gcacagccta catggaactc       60

ctcagtctga catctgaaga ctctgcagtc tattacttt                              99


<210> 8
<211> 348
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 8
gtgcagctgc tcgagtctgg acctgagctg gtgaagcctg gagcttcaat gaagatatcc       60

tgcaaggctt ctggttactc attcactgac tacaccatga actgggtgaa gcagagccat      120

ggaaagaacc ttgagtggat tggacttatt aatccttaca atggttttac tatctataat      180

cagaagttca agggcaaggc cacattaact gtagacaagt catccagcac agcctacatg      240

gaactcctca gtctgacatc tgaagactct gcagtctatt actttataag agcgcattac      300

gacgggagag ttttttgggg ccaaggcacc actctcacag tctcctcg                   348


<210> 9
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Phe Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser 
1               5                   10          


<210> 10
<211> 13
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 10
Tyr Thr Met Asn Trp Val Lys Gln Ser His Gly Lys Asn 
1               5                   10              


<210> 11
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 11
Phe Thr Ile Tyr Asn Gln Lys 
1               5           


<210> 12
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 12
Ile Arg Ala His Tyr Asp Gly Arg Val 
1               5                   


<210> 13
<211> 30
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 13
Val Gln Leu Leu Glu Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser 
1               5                   10                  15      


Met Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp 
            20                  25                  30  


<210> 14
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 14
Leu Glu Trp Ile Gly Leu Ile Asn Pro Tyr Asn Gly 
1               5                   10          


<210> 15
<211> 33
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 15
Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala 
1               5                   10                  15      


Tyr Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr 
            20                  25                  30          


Phe 
    


<210> 16
<211> 116
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 16
Val Gln Leu Leu Glu Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser 
1               5                   10                  15      


Met Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr Thr 
            20                  25                  30          


Met Asn Trp Val Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile Gly 
        35                  40                  45              


Leu Ile Asn Pro Tyr Asn Gly Phe Thr Ile Tyr Asn Gln Lys Phe Lys 
    50                  55                  60                  


Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met 
65                  70                  75                  80  


Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Phe Ile 
                85                  90                  95      


Arg Ala His Tyr Asp Gly Arg Val Phe Trp Gly Gln Gly Thr Thr Leu 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210> 17
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 17
acgttcggag gggggaccaa gctagaaata aaacgg                                 36


<210> 18
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 18
cagagccttg tacacagtaa tggaaacacc tatttacat                              39


<210> 19
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 19
ctgatctacc aagtttccaa c                                                 21


<210> 20
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 20
tgctctcaaa ttacacattt tcctccc                                           27


<210> 21
<211> 81
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 21
tgtgaactag tgatgaccca gtctccactc tccctgcctg tcagtcttgg agatcaagcc       60

tccgtctctt gcagatctag t                                                 81


<210> 22
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 22
cggtacctgc agaagccagg ccagtctcca aagctc                                 36


<210> 23
<211> 102
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 23
cgattttctg gggtcccaga caggttcagt ggcagtggat cagggacaga tttcacactc       60

aagatcacca gagtggaggc tgaggatctg ggagtttatt tc                         102


<210> 24
<211> 342
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 24
tgtgaactag tgatgaccca gtctccactc tccctgcctg tcagtcttgg agatcaagcc       60

tccgtctctt gcagatctag tcagagcctt gtacacagta atggaaacac ctatttacat      120

cggtacctgc agaagccagg ccagtctcca aagctcctga tctaccaagt ttccaaccga      180

ttttctgggg tcccagacag gttcagtggc agtggatcag ggacagattt cacactcaag      240

atcaccagag tggaggctga ggatctggga gtttatttct gctctcaaat tacacatttt      300

cctcccacgt tcggaggggg gaccaagcta gaaataaaac gg                         342


<210> 25
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 25
Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
1               5                   10          


<210> 26
<211> 13
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 26
Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His 
1               5                   10              


<210> 27
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 27
Leu Ile Tyr Gln Val Ser Asn 
1               5           


<210> 28
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 28
Cys Ser Gln Ile Thr His Phe Pro Pro 
1               5                   


<210> 29
<211> 27
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 29
Cys Glu Leu Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu 
1               5                   10                  15      


Gly Asp Gln Ala Ser Val Ser Cys Arg Ser Ser 
            20                  25          


<210> 30
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 30
Arg Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu 
1               5                   10          


<210> 31
<211> 34
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 31
Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr 
1               5                   10                  15      


Asp Phe Thr Leu Lys Ile Thr Arg Val Glu Ala Glu Asp Leu Gly Val 
            20                  25                  30          


Tyr Phe 
        


<210> 32
<211> 114
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 32
Cys Glu Leu Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu 
1               5                   10                  15      


Gly Asp Gln Ala Ser Val Ser Cys Arg Ser Ser Gln Ser Leu Val His 
            20                  25                  30          


Ser Asn Gly Asn Thr Tyr Leu His Arg Tyr Leu Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Gln Val Ser Asn Arg Phe Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys 
65                  70                  75                  80  


Ile Thr Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln 
                85                  90                  95      


Ile Thr His Phe Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys Arg 
        


<210> 33
<211> 348
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 10H9 monoclonal antibody 
      heavy chain variable region polynucleotide


<220>
<221> CDS
<222> (1)..(348)

<400> 33
gtg cag ctg ctg gaa tct gga cct gag ctg gtg aag cct gga gct tca         48
Val Gln Leu Leu Glu Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser           
1               5                   10                  15                

atg aag ata tcc tgc aag gct tct ggt tac tca ttc act gac tac acc         96
Met Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr Thr           
            20                  25                  30                    

atg aac tgg gtg aag cag agc cat gga aag aac ctt gag tgg att gga        144
Met Asn Trp Val Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile Gly           
        35                  40                  45                        

ctt att aat cct tac aat ggt ttt act atc tat aat cag aag ttc aag        192
Leu Ile Asn Pro Tyr Asn Gly Phe Thr Ile Tyr Asn Gln Lys Phe Lys           
    50                  55                  60                            

ggc aag gcc aca tta act gta gac aag tca tcc agc aca gcc tac atg        240
Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met           
65                  70                  75                  80            

gaa ctc ctc agt ctg aca tct gaa gac tct gca gtc tat tac ttt ata        288
Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Phe Ile           
                85                  90                  95                

aga gcg cat tac gac ggg aga gtt ttt tgg ggc caa ggc acc act ctc        336
Arg Ala His Tyr Asp Gly Arg Val Phe Trp Gly Gln Gly Thr Thr Leu           
            100                 105                 110                   

aca gtc tcc tcg                                                        348
Thr Val Ser Ser                                                           
        115                                                               


<210> 34
<211> 116
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 10H9 monoclonal antibody 
      heavy chain variable region polypeptide

<400> 34
Val Gln Leu Leu Glu Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser 
1               5                   10                  15      


Met Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr Thr 
            20                  25                  30          


Met Asn Trp Val Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile Gly 
        35                  40                  45              


Leu Ile Asn Pro Tyr Asn Gly Phe Thr Ile Tyr Asn Gln Lys Phe Lys 
    50                  55                  60                  


Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met 
65                  70                  75                  80  


Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Phe Ile 
                85                  90                  95      


Arg Ala His Tyr Asp Gly Arg Val Phe Trp Gly Gln Gly Thr Thr Leu 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210> 35
<211> 342
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 10H9 monoclonal antibody 
      light chain variable region polynucleotide


<220>
<221> CDS
<222> (1)..(342)

<400> 35
tgt gaa cta gtg atg acc cag tct cca ctc tcc ctg cct gtc agt ctt         48
Cys Glu Leu Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu           
1               5                   10                  15                

gga gat caa gcc tcc gtc tct tgc aga tct agt cag agc ctt gta cac         96
Gly Asp Gln Ala Ser Val Ser Cys Arg Ser Ser Gln Ser Leu Val His           
            20                  25                  30                    

agt aat gga aac acc tat tta cat cgg tac ctg cag aag cca ggc cag        144
Ser Asn Gly Asn Thr Tyr Leu His Arg Tyr Leu Gln Lys Pro Gly Gln           
        35                  40                  45                        

tct cca aag ctc ctg atc tac caa gtt tcc aac cga ttt tct ggg gtc        192
Ser Pro Lys Leu Leu Ile Tyr Gln Val Ser Asn Arg Phe Ser Gly Val           
    50                  55                  60                            

cca gac agg ttc agt ggc agt gga tca ggg aca gat ttc aca ctc aag        240
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys           
65                  70                  75                  80            

atc acc aga gtg gag gct gag gat ctg gga gtt tat ttc tgc tct caa        288
Ile Thr Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln           
                85                  90                  95                

att aca cat ttt cct ccc acg ttc gga ggg ggg acc aag cta gaa ata        336
Ile Thr His Phe Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile           
            100                 105                 110                   

aaa cgg                                                                342
Lys Arg                                                                   
                                                                          


<210> 36
<211> 114
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 10H9 monoclonal antibody 
      light chain variable region polypeptide

<400> 36
Cys Glu Leu Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu 
1               5                   10                  15      


Gly Asp Gln Ala Ser Val Ser Cys Arg Ser Ser Gln Ser Leu Val His 
            20                  25                  30          


Ser Asn Gly Asn Thr Tyr Leu His Arg Tyr Leu Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Gln Val Ser Asn Arg Phe Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys 
65                  70                  75                  80  


Ile Thr Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln 
                85                  90                  95      


Ile Thr His Phe Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys Arg 
        



