                         SEQUENCE LISTING

<110>  MacroGenics, Inc.
       Barat, Bhaswati
       Huang, Ling
       Johnson, Leslie
       Bonvini, Ezio
 
<120>  Deimmunized Serum-Binding Domains and their Use for Extending 
       Serum Half-Life

<130>  1301.0007P9I

<150>  US 61/488,725
<151>  2011-05-21

<160>  329   

<170>  PatentIn version 3.5

<210>  1
<211>  13
<212>  PRT
<213>  homo sapiens

<400>  1

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro 
1               5                   10              


<210>  2
<211>  10
<212>  PRT
<213>  homo sapiens

<400>  2

Glu Arg Lys Cys Cys Val Glu Cys Pro Pro 
1               5                   10  


<210>  3
<211>  60
<212>  PRT
<213>  homo sapiens

<400>  3

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro Arg Cys 
1               5                   10                  15      


Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 
            20                  25                  30          


Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Glu 
        35                  40                  45              


Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg 
    50                  55                  60  


<210>  4
<211>  10
<212>  PRT
<213>  homo sapiens

<400>  4

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser 
1               5                   10  


<210>  5
<211>  217
<212>  PRT
<213>  homo sapiens

<400>  5

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
            100                 105                 110         


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
        115                 120                 125             


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
    130                 135                 140                 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
145                 150                 155                 160 


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
                165                 170                 175     


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
            180                 185                 190         


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
        195                 200                 205             


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215         


<210>  6
<211>  216
<212>  PRT
<213>  homo sapiens

<400>  6

Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
1               5                   10                  15      


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
            20                  25                  30          


Val Asp Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val 
        35                  40                  45              


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
    50                  55                  60                  


Phe Asn Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln 
65                  70                  75                  80  


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly 
                85                  90                  95      


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro 
            100                 105                 110         


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
        115                 120                 125             


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
    130                 135                 140                 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
145                 150                 155                 160 


Lys Thr Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
                165                 170                 175     


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
            180                 185                 190         


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
        195                 200                 205             


Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215     


<210>  7
<211>  217
<212>  PRT
<213>  homo sapiens

<400>  7

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser His Glu Asp Pro Glu Val Gln Phe Lys Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Gln Val His Asn Ala Lys Thr Lys Pro Arg Glu Gln 
    50                  55                  60                  


Gln Phe Asn Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asn Trp Leu Asp Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln 
            100                 105                 110         


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met 
        115                 120                 125             


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
    130                 135                 140                 


Ser Asp Ile Ala Val Glu Trp Glu Ser Ser Gly Gln Pro Glu Asn Asn 
145                 150                 155                 160 


Tyr Asn Thr Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu 
                165                 170                 175     


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Ile 
            180                 185                 190         


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn Arg Phe Thr Gln 
        195                 200                 205             


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215         


<210>  8
<211>  217
<212>  PRT
<213>  homo sapiens

<400>  8

Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
            100                 105                 110         


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met 
        115                 120                 125             


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
    130                 135                 140                 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
145                 150                 155                 160 


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
                165                 170                 175     


Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val 
            180                 185                 190         


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
        195                 200                 205             


Lys Ser Leu Ser Leu Ser Leu Gly Lys 
    210                 215         


<210>  9
<211>  237
<212>  PRT
<213>  homo sapiens

<400>  9

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Phe Thr Cys Arg Thr Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Lys Glu Val Ser Glu Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys 
        115                 120                 125             


Pro Thr Gln Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu 
    130                 135                 140                 


Ser Thr Ser Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys 
145                 150                 155                 160 


Ala Leu Glu Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr 
                165                 170                 175     


Asn Pro Ala Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys 
            180                 185                 190         


Asn Gln Val Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala 
        195                 200                 205             


Thr Tyr Tyr Cys Ala Gln Ile Asn Pro Ala Trp Phe Ala Tyr Trp Gly 
    210                 215                 220                 


Gln Gly Thr Leu Val Thr Val Ser Ser Leu Gly Gly Cys 
225                 230                 235         


<210>  10
<211>  8
<212>  PRT
<213>  homo sapiens

<400>  10

Gly Gly Gly Ser Gly Gly Gly Gly 
1               5               


<210>  11
<211>  244
<212>  PRT
<213>  homo sapiens

<400>  11

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala 
        115                 120                 125             


Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser 
    130                 135                 140                 


Gly Tyr Thr Phe Thr Asn Tyr Trp Ile His Trp Val Arg Gln Ala Pro 
145                 150                 155                 160 


Gly Gln Gly Leu Glu Trp Ile Gly Val Ile Asp Pro Ser Asp Thr Tyr 
                165                 170                 175     


Pro Asn Tyr Asn Lys Lys Phe Lys Gly Arg Val Thr Met Thr Val Val 
            180                 185                 190         


Val Ser Thr Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp 
        195                 200                 205             


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Gly Asp Ser Asp Tyr Tyr 
    210                 215                 220                 


Ser Gly Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
225                 230                 235                 240 


Leu Gly Gly Cys 
                


<210>  12
<211>  240
<212>  PRT
<213>  homo sapiens

<400>  12

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Phe Thr Cys Arg Thr Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Lys Glu Val Ser Glu Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
        115                 120                 125             


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
    130                 135                 140                 


Thr Asn Tyr Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
145                 150                 155                 160 


Glu Trp Ile Gly Val Ile Asp Pro Ser Asp Thr Tyr Pro Asn Tyr Asn 
                165                 170                 175     


Lys Lys Phe Lys Gly Arg Val Thr Met Thr Val Val Val Ser Thr Ser 
            180                 185                 190         


Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val 
        195                 200                 205             


Tyr Tyr Cys Ala Arg Asn Gly Asp Ser Asp Tyr Tyr Ser Gly Met Asp 
    210                 215                 220                 


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Leu Gly Gly Cys 
225                 230                 235                 240 


<210>  13
<211>  241
<212>  PRT
<213>  homo sapiens

<400>  13

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Gln Val Thr Leu Arg Glu Ser Gly Pro 
        115                 120                 125             


Ala Leu Val Lys Pro Thr Gln Thr Leu Thr Leu Thr Cys Thr Phe Ser 
    130                 135                 140                 


Gly Phe Ser Leu Ser Thr Ser Gly Met Gly Val Gly Trp Ile Arg Gln 
145                 150                 155                 160 


Pro Pro Gly Lys Ala Leu Glu Trp Leu Ala His Ile Trp Trp Asp Asp 
                165                 170                 175     


Asp Lys Arg Tyr Asn Pro Ala Leu Lys Ser Arg Leu Thr Ile Ser Lys 
            180                 185                 190         


Asp Thr Ser Lys Asn Gln Val Val Leu Thr Met Thr Asn Met Asp Pro 
        195                 200                 205             


Val Asp Thr Ala Thr Tyr Tyr Cys Ala Gln Ile Asn Pro Ala Trp Phe 
    210                 215                 220                 


Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Leu Gly Gly 
225                 230                 235                 240 


Cys 
    


<210>  14
<211>  470
<212>  PRT
<213>  homo sapiens

<400>  14

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Phe Thr Cys Arg Thr Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Lys Glu Val Ser Glu Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys 
        115                 120                 125             


Pro Thr Gln Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu 
    130                 135                 140                 


Ser Thr Ser Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys 
145                 150                 155                 160 


Ala Leu Glu Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr 
                165                 170                 175     


Asn Pro Ala Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys 
            180                 185                 190         


Asn Gln Val Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala 
        195                 200                 205             


Thr Tyr Tyr Cys Ala Gln Ile Asn Pro Ala Trp Phe Ala Tyr Trp Gly 
    210                 215                 220                 


Gln Gly Thr Leu Val Thr Val Ser Ser Leu Gly Gly Cys Val Glu Pro 
225                 230                 235                 240 


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            260                 265                 270         


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        275                 280                 285             


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
    290                 295                 300                 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                325                 330                 335     


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            340                 345                 350         


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        355                 360                 365             


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn 
    370                 375                 380                 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
385                 390                 395                 400 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                405                 410                 415     


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            420                 425                 430         


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        435                 440                 445             


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    450                 455                 460                 


Ser Leu Ser Pro Gly Lys 
465                 470 


<210>  15
<211>  477
<212>  PRT
<213>  homo sapiens

<400>  15

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala 
        115                 120                 125             


Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser 
    130                 135                 140                 


Gly Tyr Thr Phe Thr Asn Tyr Trp Ile His Trp Val Arg Gln Ala Pro 
145                 150                 155                 160 


Gly Gln Gly Leu Glu Trp Ile Gly Val Ile Asp Pro Ser Asp Thr Tyr 
                165                 170                 175     


Pro Asn Tyr Asn Lys Lys Phe Lys Gly Arg Val Thr Met Thr Val Val 
            180                 185                 190         


Val Ser Thr Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp 
        195                 200                 205             


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Gly Asp Ser Asp Tyr Tyr 
    210                 215                 220                 


Ser Gly Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
225                 230                 235                 240 


Leu Gly Gly Cys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
                245                 250                 255     


Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu 
            260                 265                 270         


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
        275                 280                 285             


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
    290                 295                 300                 


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
305                 310                 315                 320 


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
                325                 330                 335     


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
            340                 345                 350         


Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
        355                 360                 365             


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
    370                 375                 380                 


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
385                 390                 395                 400 


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
                405                 410                 415     


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
            420                 425                 430         


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
        435                 440                 445             


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
    450                 455                 460                 


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
465                 470                 475         


<210>  16
<211>  243
<212>  PRT
<213>  homo sapiens

<400>  16

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Phe Thr Cys Arg Thr Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Lys Glu Val Ser Glu Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys 
        115                 120                 125             


Pro Thr Gln Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu 
    130                 135                 140                 


Ser Thr Ser Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys 
145                 150                 155                 160 


Ala Leu Glu Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr 
                165                 170                 175     


Asn Pro Ala Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys 
            180                 185                 190         


Asn Gln Val Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala 
        195                 200                 205             


Thr Tyr Tyr Cys Ala Gln Ile Asn Pro Ala Trp Phe Ala Tyr Trp Gly 
    210                 215                 220                 


Gln Gly Thr Leu Val Thr Val Ser Ser Leu Gly Gly Cys Phe Asn Arg 
225                 230                 235                 240 


Gly Glu Cys 
            


<210>  17
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker plus C-terminus of human IgG-kappa chain

<400>  17

Leu Gly Gly Cys Phe Asn Arg Gly Glu Cys 
1               5                   10  


<210>  18
<211>  477
<212>  PRT
<213>  homo sapiens

<400>  18

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala 
        115                 120                 125             


Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser 
    130                 135                 140                 


Gly Tyr Thr Phe Thr Asn Tyr Trp Ile His Trp Val Arg Gln Ala Pro 
145                 150                 155                 160 


Gly Gln Gly Leu Glu Trp Ile Gly Val Ile Asp Pro Ser Asp Thr Tyr 
                165                 170                 175     


Pro Asn Tyr Asn Lys Lys Phe Lys Gly Arg Val Thr Met Thr Val Val 
            180                 185                 190         


Val Ser Thr Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp 
        195                 200                 205             


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Gly Asp Ser Asp Tyr Tyr 
    210                 215                 220                 


Ser Gly Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
225                 230                 235                 240 


Leu Gly Gly Cys Ala Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
                245                 250                 255     


Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu 
            260                 265                 270         


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
        275                 280                 285             


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
    290                 295                 300                 


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
305                 310                 315                 320 


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
                325                 330                 335     


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
            340                 345                 350         


Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
        355                 360                 365             


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
    370                 375                 380                 


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
385                 390                 395                 400 


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
                405                 410                 415     


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
            420                 425                 430         


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
        435                 440                 445             


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
    450                 455                 460                 


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
465                 470                 475         


<210>  19
<211>  485
<212>  PRT
<213>  homo sapiens

<400>  19

Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            20                  25                  30          


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        35                  40                  45              


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    50                  55                  60                  


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
65                  70                  75                  80  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                85                  90                  95      


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            100                 105                 110         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        115                 120                 125             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    130                 135                 140                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
145                 150                 155                 160 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                165                 170                 175     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            180                 185                 190         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        195                 200                 205             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    210                 215                 220                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Gly Gly Ser Gly Gly Gly 
225                 230                 235                 240 


Gly Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu 
                245                 250                 255     


Gly Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Phe 
            260                 265                 270         


Asp Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro 
        275                 280                 285             


Pro Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Val Pro 
    290                 295                 300                 


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
305                 310                 315                 320 


Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser 
                325                 330                 335     


Asn Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            340                 345                 350         


Gly Gly Gly Ser Gly Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly 
        355                 360                 365             


Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala 
    370                 375                 380                 


Ser Gly Tyr Thr Phe Thr Asn Tyr Trp Ile His Trp Val Arg Gln Ala 
385                 390                 395                 400 


Pro Gly Gln Gly Leu Glu Trp Ile Gly Val Ile Asp Pro Ser Asp Thr 
                405                 410                 415     


Tyr Pro Asn Tyr Asn Lys Lys Phe Lys Gly Arg Val Thr Met Thr Val 
            420                 425                 430         


Val Val Ser Thr Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser 
        435                 440                 445             


Asp Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Gly Asp Ser Asp Tyr 
    450                 455                 460                 


Tyr Ser Gly Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser 
465                 470                 475                 480 


Ser Leu Gly Gly Cys 
                485 


<210>  20
<211>  469
<212>  PRT
<213>  homo sapiens

<400>  20

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
            100                 105                 110         


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
        115                 120                 125             


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
    130                 135                 140                 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
145                 150                 155                 160 


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
                165                 170                 175     


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
            180                 185                 190         


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
        195                 200                 205             


Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Gly Gly Ser Gly Gly Gly 
    210                 215                 220                 


Gly Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu 
225                 230                 235                 240 


Gly Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Phe 
                245                 250                 255     


Asp Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro 
            260                 265                 270         


Pro Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Val Pro 
        275                 280                 285             


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
    290                 295                 300                 


Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser 
305                 310                 315                 320 


Asn Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
                325                 330                 335     


Gly Gly Gly Ser Gly Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly 
            340                 345                 350         


Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala 
        355                 360                 365             


Ser Gly Tyr Thr Phe Thr Asn Tyr Trp Ile His Trp Val Arg Gln Ala 
    370                 375                 380                 


Pro Gly Gln Gly Leu Glu Trp Ile Gly Val Ile Asp Pro Ser Asp Thr 
385                 390                 395                 400 


Tyr Pro Asn Tyr Asn Lys Lys Phe Lys Gly Arg Val Thr Met Thr Val 
                405                 410                 415     


Val Val Ser Thr Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser 
            420                 425                 430         


Asp Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Gly Asp Ser Asp Tyr 
        435                 440                 445             


Tyr Ser Gly Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser 
    450                 455                 460                 


Ser Leu Gly Gly Cys 
465                 


<210>  21
<211>  466
<212>  PRT
<213>  homo sapiens

<400>  21

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Phe Thr Cys Arg Thr Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Lys Glu Val Ser Glu Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe 
                85                  90                  95      


Thr Phe Gly Cys Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys 
        115                 120                 125             


Pro Thr Gln Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu 
    130                 135                 140                 


Ser Thr Ser Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys 
145                 150                 155                 160 


Ala Leu Glu Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr 
                165                 170                 175     


Asn Pro Ala Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys 
            180                 185                 190         


Asn Gln Val Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala 
        195                 200                 205             


Thr Tyr Tyr Cys Ala Gln Ile Asn Pro Ala Trp Phe Ala Tyr Trp Gly 
    210                 215                 220                 


Gln Gly Thr Leu Val Thr Val Ser Ser Val Glu Pro Lys Ser Cys Asp 
225                 230                 235                 240 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
                245                 250                 255     


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
            260                 265                 270         


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
        275                 280                 285             


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
    290                 295                 300                 


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
305                 310                 315                 320 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
                325                 330                 335     


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
            340                 345                 350         


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
        355                 360                 365             


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
    370                 375                 380                 


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
385                 390                 395                 400 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
                405                 410                 415     


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
            420                 425                 430         


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
        435                 440                 445             


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
    450                 455                 460                 


Gly Lys 
465     


<210>  22
<211>  246
<212>  PRT
<213>  homo sapiens

<400>  22

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala 
        115                 120                 125             


Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser 
    130                 135                 140                 


Gly Tyr Thr Phe Thr Asn Tyr Trp Ile His Trp Val Arg Gln Ala Pro 
145                 150                 155                 160 


Gly Gln Cys Leu Glu Trp Ile Gly Val Ile Asp Pro Ser Asp Thr Tyr 
                165                 170                 175     


Pro Asn Tyr Asn Lys Lys Phe Lys Gly Arg Val Thr Met Thr Val Val 
            180                 185                 190         


Val Ser Thr Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp 
        195                 200                 205             


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Gly Asp Ser Asp Tyr Tyr 
    210                 215                 220                 


Ser Gly Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
225                 230                 235                 240 


Phe Asn Arg Gly Glu Cys 
                245     


<210>  23
<211>  6
<212>  PRT
<213>  homo sapiens

<400>  23

Phe Asn Arg Gly Glu Cys 
1               5       


<210>  24
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2B6 heavy chain variable region  CDR1

<400>  24

Asn Tyr Trp Ile His 
1               5   


<210>  25
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3H7 Heavy chain variable region CDR1

<400>  25

Asp Ala Trp Met Asp 
1               5   


<210>  26
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2B6 Heavy chain variable region  CDR2

<400>  26

Val Ile Asp Pro Ser Asp Thr Tyr Pro Asn Tyr Asn Lys Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  27
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3H7 Heavy chain variable region CDR2

<400>  27

Glu Ile Arg Asn Lys Ala Asn Asn Leu Ala Thr Tyr Tyr Ala Glu Ser 
1               5                   10                  15      


Val Lys Gly 
            


<210>  28
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2B6 Heavy chain variable region  CDR3

<400>  28

Asn Gly Asp Ser Asp Tyr Tyr Ser Gly Met Asp Tyr 
1               5                   10          


<210>  29
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3H7 heavy chain variable region  CDR3

<400>  29

Tyr Ser Pro Phe Ala Tyr 
1               5       


<210>  30
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2B6 Light chain variable region  CDR1

<400>  30

Arg Thr Ser Gln Ser Ile Gly Thr Asn Ile His 
1               5                   10      


<210>  31
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3H7 light chain variable region  CDR1

<400>  31

Arg Ala Ser Gln Glu Ile Ser Gly Tyr Leu Ser 
1               5                   10      


<210>  32
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2B6 light chain variable region  CDR2

<400>  32

Asn Val Ser Glu Ser Ile Ser 
1               5           


<210>  33
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2B6 light chain variable region  CDR2

<400>  33

Tyr Val Ser Glu Ser Ile Ser 
1               5           


<210>  34
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2B6 light chain variable region CDR2

<400>  34

Tyr Ala Ser Glu Ser Ile Ser 
1               5           


<210>  35
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3H7 light chain variable region CDR2

<400>  35

Ala Ala Ser Thr Leu Asp Ser 
1               5           


<210>  36
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2B6 Light chain variable region CDR3

<400>  36

Gln Gln Ser Asn Thr Trp Pro Phe Thr 
1               5                   


<210>  37
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3H7 light chain variable region CDR3

<400>  37

Leu Gln Tyr Val Ser Tyr Pro Tyr Thr 
1               5                   


<210>  38
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2B6 heavy chain variable region

<400>  38

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Val Ile Asp Pro Ser Asp Thr Tyr Pro Asn Tyr Asn Lys Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asn Gly Asp Ser Asp Tyr Tyr Ser Gly Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120     


<210>  39
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2B6 light chain variable region  Hu2B6VL-1

<400>  39

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Ile Thr Cys Arg Thr Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Lys Asn Val Ser Glu Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  40
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 2B6 light chain variable region Hu2B6VL-2

<400>  40

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Ile Thr Cys Arg Thr Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Lys Tyr Val Ser Glu Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  41
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 2B6 light chain variable region  Hu2B6VL-3

<400>  41

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Ile Thr Cys Arg Thr Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Lys Tyr Ala Ser Glu Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  42
<211>  107
<212>  PRT
<213>  Mus musculus

<400>  42

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Ser Leu Thr Cys Arg Ala Ser Gln Glu Ile Ser Gly Tyr 
            20                  25                  30          


Leu Ser Trp Leu Gln Gln Lys Pro Asp Gly Thr Ile Arg Arg Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Thr Leu Asp Ser Gly Val Pro Lys Arg Phe Ser Gly 
    50                  55                  60                  


Ser Trp Ser Gly Ser Asp Tyr Ser Leu Thr Ile Ser Ser Leu Glu Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Asp Tyr Tyr Cys Leu Gln Tyr Val Ser Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  43
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG binding site of FcgammaRIIB receptor

<400>  43

Lys Lys Phe Ser Arg Ser Asp Pro Asn 
1               5                   


<210>  44
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG binding site of FcgammaRIIB receptor

<400>  44

Gln Lys Phe Ser Arg Leu Asp Pro Asn 
1               5                   


<210>  45
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG binding site of FcgammaRIIB receptor

<400>  45

Gln Lys Phe Ser Arg Leu Asp Pro Thr 
1               5                   


<210>  46
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG binding site of FcgammaRIIB receptor

<400>  46

Lys Lys Phe Ser Arg Leu Asp Pro Thr 
1               5                   


<210>  47
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG binding site of FcgammaRIIB receptor

<400>  47

Gln Lys Phe Ser His Leu Asp Pro Thr 
1               5                   


<210>  48
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG binding site of FcgammaRIIB receptor

<400>  48

Lys Lys Phe Ser His Leu Asp Pro Thr 
1               5                   


<210>  49
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG binding site of FcgammaRIIB receptor

<400>  49

Gln Lys Phe Ser Arg Leu Asp Pro Asn 
1               5                   


<210>  50
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG binding site for FcgammaRIIB receptor

<400>  50

Lys Lys Phe Ser Arg Ser Asp Pro Asn 
1               5                   


<210>  51
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG binding site of FcgammaRIIB receptor

<400>  51

Gln Lys Phe Ser Arg Leu Asp Pro Thr 
1               5                   


<210>  52
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG binding site of FcgammaRIIB receptor

<400>  52

Gln Lys Phe Ser His Leu Asp Pro Thr 
1               5                   


<210>  53
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG binding site of FcgammaRIIB receptor

<400>  53

Lys Lys Phe Ser Arg Leu Asp Pro Thr 
1               5                   


<210>  54
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG binding site of FcgammaRIIB receptor

<400>  54

Lys Lys Phe Ser His Leu Asp Pro Thr 
1               5                   


<210>  55
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer  Lgh321F

<400>  55
cgagctagct ctagatgaga tcacagttct ctctac                                 36


<210>  56
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer  Lgh318R

<400>  56
gcctccgcct ccggatccgc ctcctttgat ctccaccttg gtccctc                     47


<210>  57
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Lgh319F

<400>  57
ggaggcggat ccggaggcgg aggccaggtt cagctggtgc agtctgg                     47


<210>  58
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer  Lgh320R

<400>  58
tttgaattct agcagcctcc cagtgaggag acggtgaccg tgg                         43


<210>  59
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Lgh315R

<400>  59
gcctccgcct ccggatccgc ctcctttgat ctcaagcttg gtcccc                      46


<210>  60
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Lgh316F

<400>  60
ggaggcggat ccggaggcgg aggccaggtt accctgagag agtctggc                    48


<210>  61
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Lgh317R

<400>  61
tttgaattcc tagcagcctc ccagtgagct cacagtgacc agagtcc                     47


<210>  62
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Lgh339R

<400>  62
ctcaacgcag cctcccagtg agctcac                                           27


<210>  63
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Lgh340R

<400>  63
aacgcagcct cccagtgagg agacggtgac c                                      31


<210>  64
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Lgh366R

<400>  64
tttgaattct atttacccgg agacagg                                           27


<210>  65
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Lhg367F

<400>  65
ctgggaggct gcgcagagcc caaatcttgt gac                                    33


<210>  66
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Lgh368R

<400>  66
gtcacaagat ttgggctctg cgcagcctcc cag                                    33


<210>  67
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Lgh369R

<400>  67
tttgaattct aacactctcc cctgttgaag cagcctccca gtgaggagac                  50


<210>  68
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized anti-CD16A Heavy Chain Variable Region Hu3G8VH-1

<400>  68

Gln Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys Pro Thr Gln 
1               5                   10                  15      


Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu Ser Thr Ser 
            20                  25                  30          


Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu 
        35                  40                  45              


Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr Asn Pro Ala 
    50                  55                  60                  


Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val 
65                  70                  75                  80  


Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Ile Asn Pro Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  69
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized anti-CD16A heavy chain variable region  Hu3G8VH-5

<400>  69

Gln Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys Pro Thr Gln 
1               5                   10                  15      


Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu Ser Thr Ser 
            20                  25                  30          


Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu 
        35                  40                  45              


Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr Asn Pro Ala 
    50                  55                  60                  


Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val 
65                  70                  75                  80  


Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Gln Ile Asn Pro Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  70
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized anti-CD16A heavy chain variable region Hu3G8VH-22

<400>  70

Gln Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys Pro Thr Gln 
1               5                   10                  15      


Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu Ser Thr Ser 
            20                  25                  30          


Gly Val Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu 
        35                  40                  45              


Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr Ser Pro Ser 
    50                  55                  60                  


Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val 
65                  70                  75                  80  


Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Ile Asn Pro Ala Tyr Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser 
        115         


<210>  71
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized anti-CD16A light chain variable region Hu3G8VL-1

<400>  71

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  72
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized anti-CD16A light chain variable region  Hu3G8VL-43

<400>  72

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Ser Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Ser Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  73
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized anti-CD16A light chain variable region  Hu3G8VL-22

<400>  73

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Thr Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Ser Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  74
<211>  24
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  DNA sequence of SEQ ID NO:10

<400>  74
ggaggcggat ccggaggcgg aggc                                              24


<210>  75
<211>  18
<212>  DNA
<213>  homo sapiens

<400>  75
ttcaacaggg gagagtgt                                                     18


<210>  76
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker plus C-terminus of human IgG kappa chain

<400>  76
ctgggaggct gcttcaacag gggagagtgt                                        30


<210>  77
<211>  6
<212>  PRT
<213>  homo sapiens

<400>  77

Val Glu Pro Lys Ser Cys 
1               5       


<210>  78
<211>  18
<212>  DNA
<213>  homo sapiens

<400>  78
gttgagccca aatcttgt                                                     18


<210>  79
<211>  118
<212>  PRT
<213>  homo sapiens

<400>  79

Gln Val Thr Leu Lys Glu Ser Gly Pro Gly Ile Leu Gln Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ser Phe Ser Gly Phe Ser Leu Arg Thr Ser 
            20                  25                  30          


Gly Met Gly Val Gly Trp Ile Arg Gln Pro Ser Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr Asn Pro Ala 
    50                  55                  60                  


Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Ser Asn Gln Val 
65                  70                  75                  80  


Phe Leu Lys Ile Ala Ser Val Asp Thr Ala Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Gln Ile Asn Pro Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  80
<211>  354
<212>  DNA
<213>  homo sapiens

<400>  80
caggttaccc tgaaagagtc tggccctggg atattgcagc cctcccagac cctcagtctg       60

acttgttctt tctctgggtt ttcactgagg acttctggta tgggtgtagg ctggattcgt      120

cagccttcag ggaagggtct agagtggctg gcacacattt ggtgggatga tgacaagcgc      180

tataatccag ccctgaagag ccgactgaca atctccaagg atacctccag caaccaggta      240

ttcctcaaaa tcgccagtgt ggacactgca gatactgcca catactactg tgctcaaata      300

aaccccgcct ggtttgctta ctggggccaa gggactctgg tcactgtgag ctca            354


<210>  81
<211>  354
<212>  DNA
<213>  homo sapiens

<400>  81
caggttaccc tgagagagtc tggccctgcg ctggtgaagc ccacacagac cctcacactg       60

acttgtacct tctctgggtt ttcactgagc acttctggta tgggtgtagg ctggattcgt      120

cagcctcccg ggaaggctct agagtggctg gcacacattt ggtgggatga tgacaagcgc      180

tataatccag ccctgaagag ccgactgaca atctccaagg atacctccaa aaaccaggta      240

gtcctcacaa tgaccaacat ggaccctgtg gatactgcca catactactg tgctcaaata      300

aaccccgcct ggtttgctta ctggggccaa gggactctgg tcactgtgag ctca            354


<210>  82
<211>  111
<212>  PRT
<213>  homo sapiens

<400>  82

Asp Thr Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Ala Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Glu Asp Thr Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  83
<211>  333
<212>  DNA
<213>  homo sapiens

<400>  83
gacactgtgc tgacccaatc tccagcttct ttggctgtgt ctctagggca gagggccacc       60

atctcctgca aggccagcca aagtgttgat tttgatggtg atagttttat gaactggtac      120

caacagaaac caggacagcc acccaaactc ctcatctata ctacatccaa tctagaatct      180

gggatcccag ccaggtttag tgccagtggg tctgggacag acttcaccct caacatccat      240

cctgtggagg aggaggatac tgcaacctat tactgtcagc aaagtaatga ggatccgtac      300

acgttcggag gggggaccaa gctggaaata aaa                                   333


<210>  84
<211>  332
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Humanized anti-CD16A light chain variable region Hu3G8VL-1

<400>  84
gacatcgtga tgacccaatc tccagactct ttggctgtgt ctctagggga gagggccacc       60

atcaactgca aggccagcca aagtgttgat tttgatggtg atagttttat gaactggtac      120

caacagaaac caggacagcc acccaaactc ctcatctata ctacatccaa tctagaatct      180

ggggtcccag acaggtttag tggcagtggg tctgggacag acttcaccct caccatcagc      240

agcctgcagg ctgaggatgt ggcagtttat tactgtcagc aaagtaatga ggatccgtac      300

acgttcggac aggggaccaa gcttgagatc aa                                    332


<210>  85
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 2B6 heavy chain variable region

<400>  85

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Val Ile Asp Pro Ser Asp Thr Tyr Pro Asn Tyr Asn Lys Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Val Val Val Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asn Gly Asp Ser Asp Tyr Tyr Ser Gly Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120     


<210>  86
<211>  363
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  humanized 2B6 heavy chain variable region

<400>  86
caggttcagc tggtgcagtc tggagctgag gtgaagaagc ctggggcctc agtgaaggtc       60

tcctgcaagg cttctggtta cacctttacc aactactgga tacactgggt gcgacaggcc      120

cctggacaag ggcttgagtg gattggagtg attgatcctt ctgatactta tccaaattac      180

aataaaaagt tcaagggcag agtcaccatg accgtagtcg tatccacgag cacagcctac      240

atggagctga ggagcctgag atctgacgac acggccgtgt attactgtgc gagaaacggt      300

gattccgatt attactctgg tatggactac tgggggcaag ggaccacggt caccgtctcc      360

tca                                                                    363


<210>  87
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized 2B6 light chain variable region

<400>  87

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  88
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  humanized 2B6 light chain variable region

<400>  88
gaaattgtgc tgactcagtc tccagacttt cagtctgtga ctccaaagga gaaagtcacc       60

ttcacctgca ggaccagtca gagcattggc acaaacatac actggtacca gcagaaacca      120

gatcagtctc caaagctcct catcaaggag gtttctgagt ctatctctgg agtcccatcg      180

aggttcagtg gcagtggatc tgggacagat ttcaccctca ccatcaatag cctggaagct      240

gaagatgctg caacgtatta ctgtcaacaa agtaatacct ggccgttcac gttcggcgga      300

gggaccaagg tggagatcaa a                                                321


<210>  89
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  thrombin cleavage recognition site

<400>  89

Leu Val Pro Arg Gly Ser 
1               5       


<210>  90
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Factor Xa cleavage recognition site


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa=Glu or Asp

<400>  90

Ile Xaa Gly Arg 
1               


<210>  91
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  enterokinase cleavage recognition site

<400>  91

Asp Asp Asp Asp Lys 
1               5   


<210>  92
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  furin cleavage recognition site


<220>
<221>  VARIANT
<222>  (2)..(3)
<223>  Xaa=any amino acid

<400>  92

Arg Xaa Xaa Arg 
1               


<210>  93
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  preferred furin cleavage recognition site


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa=any amino acid

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Xaa=Lys or Arg

<400>  93

Arg Xaa Xaa Arg 
1               


<210>  94
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AcTEV cleavage recognition site

<400>  94

Gln Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  95
<211>  487
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  covalent diabody polyprotein precursor

<400>  95

Asp Thr Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Ala Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Glu Asp Thr Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Glu Val Glu Leu Val Glu Ser Gly Gly 
        115                 120                 125             


Gly Leu Val Gln Pro Gly Arg Ser Leu Lys Leu Ser Cys Ala Ala Ser 
    130                 135                 140                 


Gly Phe Thr Phe Ser Asp Tyr Tyr Met Ala Trp Val Arg Gln Ala Pro 
145                 150                 155                 160 


Thr Thr Gly Leu Glu Trp Val Ala Ser Ile Ser Tyr Asp Gly Gly Asp 
                165                 170                 175     


Thr His Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
            180                 185                 190         


Asn Ala Lys Ser Ser Leu Tyr Leu Gln Met Asp Ser Leu Arg Ser Glu 
        195                 200                 205             


Asp Thr Ala Thr Tyr Tyr Cys Ala Thr Glu Thr Thr Gly Ile Pro Thr 
    210                 215                 220                 


Gly Val Met Asp Ala Trp Gly Gln Gly Val Ser Val Thr Val Ser Ser 
225                 230                 235                 240 


Leu Gly Gly Cys Gly Gly Arg Ala Lys Arg Asp Val Gln Met Thr Gln 
                245                 250                 255     


Ser Pro Ser Asn Leu Ala Ala Ser Pro Gly Glu Ser Val Ser Ile Asn 
            260                 265                 270         


Cys Lys Ala Ser Glu Ser Ile Ser Lys Tyr Leu Ala Trp Tyr Leu Gln 
        275                 280                 285             


Lys Pro Gly Lys Ala Asn Lys Leu Leu Met Tyr Asp Gly Ser Thr Leu 
    290                 295                 300                 


Gln Ser Gly Ile Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
305                 310                 315                 320 


Phe Thr Leu Thr Ile Arg Ser Leu Glu Pro Glu Asp Phe Gly Leu Tyr 
                325                 330                 335     


Tyr Cys Gln Gln His Tyr Glu Tyr Pro Ala Thr Phe Gly Ser Gly Thr 
            340                 345                 350         


Lys Leu Glu Ile Lys Gly Gly Gly Ser Gly Gly Gly Gly Gln Val Thr 
        355                 360                 365             


Leu Lys Glu Ser Gly Pro Gly Ile Leu Gln Pro Ser Gln Thr Leu Ser 
    370                 375                 380                 


Leu Thr Cys Ser Phe Ser Gly Phe Ser Leu Arg Thr Ser Gly Met Gly 
385                 390                 395                 400 


Val Gly Trp Ile Arg Gln Pro Ser Gly Lys Gly Leu Glu Trp Leu Ala 
                405                 410                 415     


His Ile Trp Trp Asp Asp Asp Lys Arg Tyr Asn Pro Ala Leu Lys Ser 
            420                 425                 430         


Arg Leu Thr Ile Ser Lys Asp Thr Ser Ser Asn Gln Val Phe Leu Lys 
        435                 440                 445             


Ile Ala Ser Val Asp Thr Ala Asp Thr Ala Thr Tyr Tyr Cys Ala Gln 
    450                 455                 460                 


Ile Asn Pro Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr 
465                 470                 475                 480 


Val Ser Ser Leu Gly Gly Cys 
                485         


<210>  96
<211>  1464
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of SEQ ID NO:97 (covalent diabody polyprotein 
       precursor)

<400>  96
gacactgtgc tgacccaatc tccagcttct ttggctgtgt ctctagggca gagggccacc       60

atctcctgca aggccagcca aagtgttgat tttgatggtg atagttttat gaactggtac      120

caacagaaac caggacagcc acccaaactc ctcatctata ctacatccaa tctagaatct      180

gggatcccag ccaggtttag tgccagtggg tctgggacag acttcaccct caacatccat      240

cctgtggagg aggaggatac tgcaacctat tactgtcagc aaagtaatga ggatccgtac      300

acgttcggag gggggaccaa gctggaaata aaaggaggcg gatccggagg cggaggcgag      360

gtggagctag tggagtctgg gggaggctta gtgcagcctg gaaggtccct gaaactctcg      420

tgtgcagcct caggattcac tttcagtgac tattacatgg cctgggtccg gcaggctcca      480

acgacgggtc tggagtgggt cgcatccatt agttatgatg gtggtgacac tcactatcga      540

gactccgtga agggccgatt tactatttcc agagataatg caaaaagcag cctatacctg      600

caaatggaca gtctgaggtc tgaggacacg gccacttatt actgtgcaac agagactacg      660

ggaataccta caggtgttat ggatgcctgg ggtcaaggag tttcagtcac tgtctcctca      720

ctgggaggct gcggcgggag agctaagagg gatgtccaga tgacccagtc tccatctaat      780

cttgctgcct ctcctggaga aagtgtttcc atcaattgca aggcaagtga gagcattagc      840

aagtatttag cctggtatct acagaaacct gggaaagcaa ataagcttct tatgtacgat      900

gggtcaactt tgcaatctgg aattccatcg aggttcagtg gcagtggatc tggtacagat      960

ttcactctca ccatcagaag cctggagcct gaagattttg gactctatta ctgtcaacag     1020

cattatgaat atccagccac gttcggttct gggaccaagc tggagatcaa aggaggcgga     1080

tccggaggcg gaggccaggt taccctgaaa gagtctggcc ctgggatatt gcagccctcc     1140

cagaccctca gtctgacttg ttctttctct gggttttcac tgaggacttc tggtatgggt     1200

gtaggctgga ttcgtcagcc ttcagggaag ggtctagagt ggctggcaca catttggtgg     1260

gatgatgaca agcgctataa tccagccctg aagagccgac tgacaatctc caaggatacc     1320

tccagcaacc aggtattcct caaaatcgcc agtgtggaca ctgcagatac tgccacatac     1380

tactgtgctc aaataaaccc cgcctggttt gcttactggg gccaagggac tctggtcact     1440

gtgagctcac tgggaggctg ctag                                            1464


<210>  97
<211>  511
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  covalent diabody polyprotein precursor

<400>  97

Asp Thr Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Ala Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Glu Asp Thr Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Glu Val Glu Leu Val Glu Ser Gly Gly 
        115                 120                 125             


Gly Leu Val Gln Pro Gly Arg Ser Leu Lys Leu Ser Cys Ala Ala Ser 
    130                 135                 140                 


Gly Phe Thr Phe Ser Asp Tyr Tyr Met Ala Trp Val Arg Gln Ala Pro 
145                 150                 155                 160 


Thr Thr Gly Leu Glu Trp Val Ala Ser Ile Ser Tyr Asp Gly Gly Asp 
                165                 170                 175     


Thr His Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
            180                 185                 190         


Asn Ala Lys Ser Ser Leu Tyr Leu Gln Met Asp Ser Leu Arg Ser Glu 
        195                 200                 205             


Asp Thr Ala Thr Tyr Tyr Cys Ala Thr Glu Thr Thr Gly Ile Pro Thr 
    210                 215                 220                 


Gly Val Met Asp Ala Trp Gly Gln Gly Val Ser Val Thr Val Ser Ser 
225                 230                 235                 240 


Leu Gly Gly Cys Gly Gly Arg Ala Lys Arg Ala Pro Val Lys Gln Thr 
                245                 250                 255     


Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 
            260                 265                 270         


Gly Pro Asp Val Gln Met Thr Gln Ser Pro Ser Asn Leu Ala Ala Ser 
        275                 280                 285             


Pro Gly Glu Ser Val Ser Ile Asn Cys Lys Ala Ser Glu Ser Ile Ser 
    290                 295                 300                 


Lys Tyr Leu Ala Trp Tyr Leu Gln Lys Pro Gly Lys Ala Asn Lys Leu 
305                 310                 315                 320 


Leu Met Tyr Asp Gly Ser Thr Leu Gln Ser Gly Ile Pro Ser Arg Phe 
                325                 330                 335     


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Arg Ser Leu 
            340                 345                 350         


Glu Pro Glu Asp Phe Gly Leu Tyr Tyr Cys Gln Gln His Tyr Glu Tyr 
        355                 360                 365             


Pro Ala Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys Gly Gly Gly 
    370                 375                 380                 


Ser Gly Gly Gly Gly Gln Val Thr Leu Lys Glu Ser Gly Pro Gly Ile 
385                 390                 395                 400 


Leu Gln Pro Ser Gln Thr Leu Ser Leu Thr Cys Ser Phe Ser Gly Phe 
                405                 410                 415     


Ser Leu Arg Thr Ser Gly Met Gly Val Gly Trp Ile Arg Gln Pro Ser 
            420                 425                 430         


Gly Lys Gly Leu Glu Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys 
        435                 440                 445             


Arg Tyr Asn Pro Ala Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr 
    450                 455                 460                 


Ser Ser Asn Gln Val Phe Leu Lys Ile Ala Ser Val Asp Thr Ala Asp 
465                 470                 475                 480 


Thr Ala Thr Tyr Tyr Cys Ala Gln Ile Asn Pro Ala Trp Phe Ala Tyr 
                485                 490                 495     


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Leu Gly Gly Cys 
            500                 505                 510     


<210>  98
<211>  1536
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of SEQ ID NO:99 (covalent diabody polyprotein 
       precursor)

<400>  98
gacactgtgc tgacccaatc tccagcttct ttggctgtgt ctctagggca gagggccacc       60

atctcctgca aggccagcca aagtgttgat tttgatggtg atagttttat gaactggtac      120

caacagaaac caggacagcc acccaaactc ctcatctata ctacatccaa tctagaatct      180

gggatcccag ccaggtttag tgccagtggg tctgggacag acttcaccct caacatccat      240

cctgtggagg aggaggatac tgcaacctat tactgtcagc aaagtaatga ggatccgtac      300

acgttcggag gggggaccaa gctggaaata aaaggaggcg gatccggagg cggaggcgag      360

gtggagctag tggagtctgg gggaggctta gtgcagcctg gaaggtccct gaaactctcg      420

tgtgcagcct caggattcac tttcagtgac tattacatgg cctgggtccg gcaggctcca      480

acgacgggtc tggagtgggt cgcatccatt agttatgatg gtggtgacac tcactatcga      540

gactccgtga agggccgatt tactatttcc agagataatg caaaaagcag cctatacctg      600

caaatggaca gtctgaggtc tgaggacacg gccacttatt actgtgcaac agagactacg      660

ggaataccta caggtgttat ggatgcctgg ggtcaaggag tttcagtcac tgtctcctca      720

ctgggaggct gcggcgggag agctaagagg gcccctgtga agcagaccct gaacttcgac      780

ctgctgaagc tggccggaga cgtggagagc aaccccggcc ccgatgtcca gatgacccag      840

tctccatcta atcttgctgc ctctcctgga gaaagtgttt ccatcaattg caaggcaagt      900

gagagcatta gcaagtattt agcctggtat ctacagaaac ctgggaaagc aaataagctt      960

cttatgtacg atgggtcaac tttgcaatct ggaattccat cgaggttcag tggcagtgga     1020

tctggtacag atttcactct caccatcaga agcctggagc ctgaagattt tggactctat     1080

tactgtcaac agcattatga atatccagcc acgttcggtt ctgggaccaa gctggagatc     1140

aaaggaggcg gatccggagg cggaggccag gttaccctga aagagtctgg ccctgggata     1200

ttgcagccct cccagaccct cagtctgact tgttctttct ctgggttttc actgaggact     1260

tctggtatgg gtgtaggctg gattcgtcag ccttcaggga agggtctaga gtggctggca     1320

cacatttggt gggatgatga caagcgctat aatccagccc tgaagagccg actgacaatc     1380

tccaaggata cctccagcaa ccaggtattc ctcaaaatcg ccagtgtgga cactgcagat     1440

actgccacat actactgtgc tcaaataaac cccgcctggt ttgcttactg gggccaaggg     1500

actctggtca ctgtgagctc actgggaggc tgctag                               1536


<210>  99
<211>  239
<212>  PRT
<213>  homo sapiens

<400>  99

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Phe Thr Cys Arg Thr Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Lys Glu Val Ser Glu Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys 
        115                 120                 125             


Pro Thr Gln Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu 
    130                 135                 140                 


Ser Thr Ser Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys 
145                 150                 155                 160 


Ala Leu Glu Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr 
                165                 170                 175     


Asn Pro Ala Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys 
            180                 185                 190         


Asn Gln Val Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala 
        195                 200                 205             


Thr Tyr Tyr Cys Ala Gln Ile Asn Pro Ala Trp Phe Ala Tyr Trp Gly 
    210                 215                 220                 


Gln Gly Thr Leu Val Thr Val Ser Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235                 


<210>  100
<211>  720
<212>  DNA
<213>  homo sapiens

<400>  100
gaaattgtgc tgactcagtc tccagacttt cagtctgtga ctccaaagga gaaagtcacc       60

ttcacctgca ggaccagtca gagcattggc acaaacatac actggtacca gcagaaacca      120

gatcagtctc caaagctcct catcaaggag gtttctgagt ctatctctgg agtcccatcg      180

aggttcagtg gcagtggatc tgggacagat ttcaccctca ccatcaatag cctggaagct      240

gaagatgctg caacgtatta ctgtcaacaa agtaatacct ggccgttcac gttcggcgga      300

gggaccaagg tggagatcaa aggaggcgga tccggaggcg gaggccaggt taccctgaga      360

gagtctggcc ctgcgctggt gaagcccaca cagaccctca cactgacttg taccttctct      420

gggttttcac tgagcacttc tggtatgggt gtaggctgga ttcgtcagcc tcccgggaag      480

gctctagagt ggctggcaca catttggtgg gatgatgaca agcgctataa tccagccctg      540

aagagccgac tgacaatctc caaggatacc tccaaaaacc aggtagtcct cacaatgacc      600

aacatggacc ctgtggatac tgccacatac tactgtgctc aaataaaccc cgcctggttt      660

gcttactggg gccaagggac tctggtcact gtgagctcat tcaacagggg agagtgttag      720


<210>  101
<211>  246
<212>  PRT
<213>  Homo sapiens

<400>  101

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala 
        115                 120                 125             


Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser 
    130                 135                 140                 


Gly Tyr Thr Phe Thr Asn Tyr Trp Ile His Trp Val Arg Gln Ala Pro 
145                 150                 155                 160 


Gly Gln Gly Leu Glu Trp Ile Gly Val Ile Asp Pro Ser Asp Thr Tyr 
                165                 170                 175     


Pro Asn Tyr Asn Lys Lys Phe Lys Gly Arg Val Thr Met Thr Val Val 
            180                 185                 190         


Val Ser Thr Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp 
        195                 200                 205             


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Gly Asp Ser Asp Tyr Tyr 
    210                 215                 220                 


Ser Gly Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
225                 230                 235                 240 


Val Glu Pro Lys Ser Cys 
                245     


<210>  102
<211>  741
<212>  DNA
<213>  Homo sapiens

<400>  102
gacatcgtga tgacccaatc tccagactct ttggctgtgt ctctagggga gagggccacc       60

atcaactgca aggccagcca aagtgttgat tttgatggtg atagttttat gaactggtac      120

caacagaaac caggacagcc acccaaactc ctcatctata ctacatccaa tctagaatct      180

ggggtcccag acaggtttag tggcagtggg tctgggacag acttcaccct caccatcagc      240

agcctgcagg ctgaggatgt ggcagtttat tactgtcagc aaagtaatga ggatccgtac      300

acgttcggac aggggaccaa gcttgagatc aaaggaggcg gatccggagg cggaggccag      360

gttcagctgg tgcagtctgg agctgaggtg aagaagcctg gggcctcagt gaaggtctcc      420

tgcaaggctt ctggttacac ctttaccaac tactggatac actgggtgcg acaggcccct      480

ggacaagggc ttgagtggat tggagtgatt gatccttctg atacttatcc aaattacaat      540

aaaaagttca agggcagagt caccatgacc gtagtcgtat ccacgagcac agcctacatg      600

gagctgagga gcctgagatc tgacgacacg gccgtgtatt actgtgcgag aaacggtgat      660

tccgattatt actctggtat ggactactgg gggcaaggga ccacggtcac cgtctcctca      720

gttgagccca aatcttgtta g                                                741


<210>  103
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH FR1

<400>  103

Gln Val Thr Leu Lys Glu Ser Gly Pro Gly Ile Leu Gln Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ser Phe Ser Gly Phe Ser Leu Arg 
            20                  25                  30  


<210>  104
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH FR1

<400>  104

Gln Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys Pro Thr Gln 
1               5                   10                  15      


Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu Ser 
            20                  25                  30  


<210>  105
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH FR1

<400>  105

Gln Val Thr Leu Lys Glu Ser Gly Pro Ala Leu Val Lys Pro Thr Gln 
1               5                   10                  15      


Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu Ser 
            20                  25                  30  


<210>  106
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH FR1

<400>  106

Gln Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys Pro Thr Gln 
1               5                   10                  15      


Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu Arg 
            20                  25                  30  


<210>  107
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH FR1

<400>  107

Gln Ile Thr Leu Lys Glu Ser Gly Pro Thr Leu Val Lys Pro Thr Gln 
1               5                   10                  15      


Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu Ser 
            20                  25                  30  


<210>  108
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH *  CDR1

<400>  108

Thr Ser Gly Met Gly Val Gly 
1               5           


<210>  109
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH *  CDR1

<400>  109

Thr Ser Gly Val Gly Val Gly 
1               5           


<210>  110
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR2

<400>  110

Trp Ile Arg Gln Pro Ser Gly Lys Gly Leu Glu Trp Leu Ala 
1               5                   10                  


<210>  111
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR2

<400>  111

Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu Trp Leu Ala 
1               5                   10                  


<210>  112
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR2

<400>  112

His Ile Trp Trp Asp Asp Asp Lys Arg Tyr Asn Pro Ala Leu Lys Ser 
1               5                   10                  15      


<210>  113
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR2

<400>  113

His Ile Tyr Trp Asn Asp Asp Lys Arg Tyr Asn Pro Ala Leu Lys Ser 
1               5                   10                  15      


<210>  114
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR2

<400>  114

His Ile Trp Trp Asp Asp Asp Lys Arg Tyr Ser Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  115
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR2

<400>  115

His Ile Tyr Trp Asp Asp Asp Lys Arg Tyr Asn Pro Ala Leu Lys Ser 
1               5                   10                  15      


<210>  116
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR2

<400>  116

His Ile Trp Trp Asn Asp Asp Lys Arg Tyr Asn Pro Ala Leu Lys Ser 
1               5                   10                  15      


<210>  117
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR2

<400>  117

Leu Ile Asp Trp Asp Asp Asp Lys Arg Tyr Ser Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  118
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR2

<400>  118

His Ile Phe Trp Asp Asp Asp Lys Arg Tyr Ser Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  119
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR2

<400>  119

Leu Ile Trp Trp Asp Asp Asp Lys Arg Tyr Ser Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  120
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR2

<400>  120

His Ile Asp Trp Asp Asp Asp Lys Arg Tyr Ser Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  121
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR2

<400>  121

Leu Ile Trp Trp Asn Asp Asp Lys Arg Tyr Ser Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  122
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  122

Arg Leu Thr Ile Ser Lys Asp Thr Ser Ser Asn Gln Val Phe Leu Lys 
1               5                   10                  15      


Ile Asp Thr Ala Asp Thr Ala Thr Tyr Tyr Cys Ala Gln 
            20                  25                  


<210>  123
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  123

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Arg 
            20                  25                  


<210>  124
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  124

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Gln 
            20                  25                  


<210>  125
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  125

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Thr 
            20                  25                  


<210>  126
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  126

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Lys 
            20                  25                  


<210>  127
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  127

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Ala 
            20                  25                  


<210>  128
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  128

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala His 
            20                  25                  


<210>  129
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  129

Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Arg 
            20                  25                  


<210>  130
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  130

Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala His 
            20                  25                  


<210>  131
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  131

Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Gln 
            20                  25                  


<210>  132
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  132

Arg Leu Thr Ile Ser Lys Asp Thr Ser Ser Asn Gln Val Phe Leu Lys 
1               5                   10                  15      


Ala Asp Thr Ala Asp Thr Ala Thr Tyr Tyr Cys Ala Gln 
            20                  25                  


<210>  133
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  133

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Thr Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Arg 
            20                  25                  


<210>  134
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  134

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Thr Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Gln 
            20                  25                  


<210>  135
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  135

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Thr Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Thr 
            20                  25                  


<210>  136
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  136

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Thr Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Lys 
            20                  25                  


<210>  137
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  137

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Thr Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Ala 
            20                  25                  


<210>  138
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  138

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Thr Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala His 
            20                  25                  


<210>  139
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  139

Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Thr Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Arg 
            20                  25                  


<210>  140
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  140

Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Thr Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala His 
            20                  25                  


<210>  141
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  141

Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Thr Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Gln 
            20                  25                  


<210>  142
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  142

Arg Leu Thr Ile Ser Lys Asp Thr Ser Ser Asn Gln Val Phe Leu Lys 
1               5                   10                  15      


Ser Asp Thr Ala Asp Thr Ala Thr Tyr Tyr Cys Ala Gln 
            20                  25                  


<210>  143
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  143

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Asn Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Arg 
            20                  25                  


<210>  144
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  144

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Asn Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Gln 
            20                  25                  


<210>  145
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  145

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Asn Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Thr 
            20                  25                  


<210>  146
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  146

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Asn Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Lys 
            20                  25                  


<210>  147
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  147

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Asn Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Ala 
            20                  25                  


<210>  148
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  148

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Asn Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala His 
            20                  25                  


<210>  149
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  149

Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Asn Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Arg 
            20                  25                  


<210>  150
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  150

Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Asn Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala His 
            20                  25                  


<210>  151
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  151

Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Asn Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Gln 
            20                  25                  


<210>  152
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  152

Arg Leu Thr Ile Ser Lys Asp Thr Ser Ser Asn Gln Val Phe Leu Lys 
1               5                   10                  15      


Val Asp Thr Ala Asp Thr Ala Thr Tyr Tyr Cys Ala Gln 
            20                  25                  


<210>  153
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  153

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Arg 
            20                  25                  


<210>  154
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  154

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Gln 
            20                  25                  


<210>  155
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  155

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Thr 
            20                  25                  


<210>  156
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  156

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Lys 
            20                  25                  


<210>  157
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  157

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Ala 
            20                  25                  


<210>  158
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  158

Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala His 
            20                  25                  


<210>  159
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  159

Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Arg 
            20                  25                  


<210>  160
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  160

Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala His 
            20                  25                  


<210>  161
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR3

<400>  161

Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr 
1               5                   10                  15      


Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Gln 
            20                  25                  


<210>  162
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR3

<400>  162

Ile Asn Pro Ala Trp Phe Ala Tyr 
1               5               


<210>  163
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR3

<400>  163

Ile Asn Pro Ala Trp Phe Asp Tyr 
1               5               


<210>  164
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR3

<400>  164

Ile Asn Pro Ala Tyr Phe Ala Tyr 
1               5               


<210>  165
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * CDR3

<400>  165

Ile Asn Pro Ala Tyr Phe Asp Tyr 
1               5               


<210>  166
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR4

<400>  166

Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala 
1               5                   10      


<210>  167
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH Sequences Derived from 3G8 VH * FR4

<400>  167

Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
1               5                   10      


<210>  168
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL* FR1

<400>  168

Asp Thr Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu 
1               5                   10                  15  


<210>  169
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL* FR1

<400>  169

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu 
1               5                   10                  15  


<210>  170
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  170

Lys Ala Ser Gln Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  171
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  171

Arg Ala Ser Gln Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  172
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  172

Lys Ser Ser Gln Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  173
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  173

Lys Ala Ser Gln Asp Gly Asp Ser Tyr Met Asn 
1               5                   10      


<210>  174
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  174

Lys Ala Ser Gln Asp Gly Asp Ser Phe Leu Asn 
1               5                   10      


<210>  175
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  175

Lys Ala Ser Gln Asp Gly Asp Ser Phe Met Ala 
1               5                   10      


<210>  176
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  176

Lys Ala Ser Gln Asp Gly Asp Ser Tyr Leu Ala 
1               5                   10      


<210>  177
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  177

Lys Ala Ser Ser Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  178
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  178

Arg Ala Ser Ser Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  179
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  179

Lys Ser Ser Ser Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  180
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  180

Lys Ala Ser Ser Asp Gly Asp Ser Tyr Met Asn 
1               5                   10      


<210>  181
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  181

Lys Ala Ser Ser Asp Gly Asp Ser Phe Leu Asn 
1               5                   10      


<210>  182
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  182

Lys Ala Ser Ser Asp Gly Asp Ser Phe Met Ala 
1               5                   10      


<210>  183
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  183

Lys Ala Ser Ser Asp Gly Asp Ser Tyr Leu Ala 
1               5                   10      


<210>  184
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  184

Lys Ala Ser Val Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  185
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  185

Arg Ala Ser Val Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  186
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  186

Lys Ser Ser Val Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  187
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  187

Lys Ala Ser Val Asp Gly Asp Ser Tyr Met Asn 
1               5                   10      


<210>  188
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  188

Lys Ala Ser Val Asp Gly Asp Ser Phe Leu Asn 
1               5                   10      


<210>  189
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  189

Lys Ala Ser Val Asp Gly Asp Ser Phe Met Ala 
1               5                   10      


<210>  190
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  190

Lys Ala Ser Val Asp Gly Asp Ser Tyr Leu Ala 
1               5                   10      


<210>  191
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  191

Lys Ala Ser Asp Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  192
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  192

Arg Ala Ser Asp Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  193
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  193

Lys Ser Ser Asp Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  194
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  194

Lys Ala Ser Asp Asp Gly Asp Ser Tyr Met Asn 
1               5                   10      


<210>  195
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  195

Lys Ala Ser Asp Asp Gly Asp Ser Phe Leu Asn 
1               5                   10      


<210>  196
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  196

Lys Ala Ser Asp Asp Gly Asp Ser Phe Met Ala 
1               5                   10      


<210>  197
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  197

Lys Ala Ser Asp Asp Gly Asp Ser Tyr Leu Ala 
1               5                   10      


<210>  198
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  198

Lys Ala Ser Phe Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  199
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  199

Arg Ala Ser Phe Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  200
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  200

Lys Ser Ser Phe Asp Gly Asp Ser Phe Met Asn 
1               5                   10      


<210>  201
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  201

Lys Ala Ser Phe Asp Gly Asp Ser Tyr Met Asn 
1               5                   10      


<210>  202
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  202

Lys Ala Ser Phe Asp Gly Asp Ser Phe Leu Asn 
1               5                   10      


<210>  203
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  203

Lys Ala Ser Phe Asp Gly Asp Ser Phe Met Ala 
1               5                   10      


<210>  204
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR1

<400>  204

Lys Ala Ser Phe Asp Gly Asp Ser Tyr Leu Ala 
1               5                   10      


<210>  205
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  FR2

<400>  205

Trp Tyr Gln Gln Lys Ala Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
1               5                   10                  15      


<210>  206
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR2

<400>  206

Thr Thr Ser Asn Leu Glu Ser 
1               5           


<210>  207
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR2

<400>  207

Asp Ala Ser Asn Leu Glu Ser 
1               5           


<210>  208
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR2

<400>  208

Trp Ala Ser Asn Leu Glu Ser 
1               5           


<210>  209
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR2

<400>  209

Thr Thr Ser Asn Leu Glu Thr 
1               5           


<210>  210
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR2

<400>  210

Asp Ala Ser Asn Leu Glu Thr 
1               5           


<210>  211
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR2

<400>  211

Asp Ala Ser Asn Leu Ala Thr 
1               5           


<210>  212
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR2

<400>  212

Ser Ala Ser Asn Leu Gln Ser 
1               5           


<210>  213
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR2

<400>  213

Ser Thr Ser Asn Leu Glu Ser 
1               5           


<210>  214
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR2

<400>  214

Ser Thr Ser Asn Leu Gln Ser 
1               5           


<210>  215
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR2

<400>  215

Thr Thr Ser Asn Leu Gln Ser 
1               5           


<210>  216
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR2

<400>  216

Thr Thr Ser Ser Leu Gln Ser 
1               5           


<210>  217
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  FR3

<400>  217

Gly Ile Pro Ala Arg Phe Ser Ala Ser Gly Ser Gly Thr Asp Phe Thr 
1               5                   10                  15      


Leu Asn Ile His Pro Val Glu Glu Glu Asp Thr Ala Thr Tyr Tyr Cys 
            20                  25                  30          


<210>  218
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  FR3

<400>  218

Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
1               5                   10                  15      


Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys 
            20                  25                  30          


<210>  219
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR3

<400>  219

Gln Gln Ser Asn Glu Asp Pro Tyr Thr 
1               5                   


<210>  220
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR3

<400>  220

Gln Gln Ser Tyr Ser Thr Pro Tyr Thr 
1               5                   


<210>  221
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR3

<400>  221

Gln Gln Ser Tyr Glu Asp Pro Tyr Thr 
1               5                   


<210>  222
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR3

<400>  222

Gln Gln Ser Asn Ser Asp Pro Tyr Thr 
1               5                   


<210>  223
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  CDR3

<400>  223

Gln Gln Ser Asn Glu Thr Pro Tyr Thr 
1               5                   


<210>  224
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  FR4

<400>  224

Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
1               5                   10  


<210>  225
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL Sequences Derived from 3G8 VL*  FR4

<400>  225

Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
1               5                   10  


<210>  226
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H3G8VL-CB3.1VH

<400>  226
gacatcgtga tgacccaatc tccagactct ttggctgtgt ctctagggga gagggccacc       60

atcaactgca aggccagcca aagtgttgat tttgatggtg atagttttat gaactggtac      120

caacagaaac caggacagcc acccaaactc ctcatctata ctacatccaa tctagaatct      180

ggggtcccag acaggtttag tggcagtggg tctgggacag acttcaccct caccatcagc      240

agcctgcagg ctgaggatgt ggcagtttat tactgtcagc aaagtaatga ggatccgtac      300

acgttcggac aggggaccaa gcttgagatc aaaggaggcg gatccggagg cggaggccag      360

gtccaactgc agcagcctgg ggctgagctg gtgaggcctg gggcttcagt gaagctgtcc      420

tgcaaggctt ctggctacac cttcaccagc tactggatga actgggtgaa gcagaggcct      480

ggacaaggcc ttgaatggat tggtatggtt gatccttcag acagtgaaac tcactacaat      540

caaatgttca aggacaaggc cacattgact gttgacaaat cctccagcac agcctacatg      600

cagctcagca gcctgacatc tgaggactct gcggtctatt actgtgcaag agctatgggc      660

tactggggtc aaggaacctc agtcaccgtc tcctcagttg agcccaaatc ttgt            714


<210>  227
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H3G8VL-CB3.1VH

<400>  227

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Gln Val Gln Leu Gln Gln Pro Gly Ala 
        115                 120                 125             


Glu Leu Val Arg Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser 
    130                 135                 140                 


Gly Tyr Thr Phe Thr Ser Tyr Trp Met Asn Trp Val Lys Gln Arg Pro 
145                 150                 155                 160 


Gly Gln Gly Leu Glu Trp Ile Gly Met Val Asp Pro Ser Asp Ser Glu 
                165                 170                 175     


Thr His Tyr Asn Gln Met Phe Lys Asp Lys Ala Thr Leu Thr Val Asp 
            180                 185                 190         


Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu 
        195                 200                 205             


Asp Ser Ala Val Tyr Tyr Cys Ala Arg Ala Met Gly Tyr Trp Gly Gln 
    210                 215                 220                 


Gly Thr Ser Val Thr Val Ser Ser Val Glu Pro Lys Ser Cys 
225                 230                 235             


<210>  228
<211>  732
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CB3.1VL-h3G8VH

<400>  228
gatgttgtga tgacccagac tccactcact ttgtcggtta acattggaca accagcctcc       60

atctcttgta agtcaagtca gagcctctta gatactgatg gaaagacata tttgaattgg      120

ttgttacaga ggccaggcca gtctccaaac cgcctaatct atctggtgtc taaactggac      180

tctggagtcc ctgacaggtt cactggcagt ggatcaggga cagatttcac actgaaaatc      240

agcagagtgg aggctgagga tttgggaatt tattattgct ggcaaggtac acattttccg      300

ctcacgttcg gtgctgggac caagctggag ctgaaaggag gcggatccgg aggcggaggc      360

caggttaccc tgagagagtc tggccctgcg ctggtgaagc ccacacagac cctcacactg      420

acttgtacct tctctgggtt ttcactgagc acttctggta tgggtgtagg ctggattcgt      480

cagcctcccg ggaaggctct agagtggctg gcacacattt ggtgggatga tgacaagcgc      540

tataatccag ccctgaagag ccgactgaca atctccaagg atacctccaa aaaccaggta      600

gtcctcacaa tgaccaacat ggaccctgtg gatactgcca catactactg tgctcaaata      660

aaccccgcct ggtttgctta ctggggccaa gggactctgg tcactgtgag ctcattcaac      720

aggggagagt gt                                                          732


<210>  229
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CB3.1VL-h3G8VH

<400>  229

Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser Val Asn Ile Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Thr 
            20                  25                  30          


Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Asn Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Ile Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Gln Val Thr Leu Arg Glu Ser Gly 
        115                 120                 125             


Pro Ala Leu Val Lys Pro Thr Gln Thr Leu Thr Leu Thr Cys Thr Phe 
    130                 135                 140                 


Ser Gly Phe Ser Leu Ser Thr Ser Gly Met Gly Val Gly Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Gly Lys Ala Leu Glu Trp Leu Ala His Ile Trp Trp Asp 
                165                 170                 175     


Asp Asp Lys Arg Tyr Asn Pro Ala Leu Lys Ser Arg Leu Thr Ile Ser 
            180                 185                 190         


Lys Asp Thr Ser Lys Asn Gln Val Val Leu Thr Met Thr Asn Met Asp 
        195                 200                 205             


Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala Gln Ile Asn Pro Ala Trp 
    210                 215                 220                 


Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Phe Asn 
225                 230                 235                 240 


Arg Gly Glu Cys 
                


<210>  230
<211>  702
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  8B5-CB3.1-VEPKSC

<400>  230
gacattcaga tgacacagtc tccatcctcc ctacttgcgg cgctgggaga aagagtcagt       60

ctcacttgtc gggcaagtca ggaaattagt ggttacttaa gctggcttca gcagaaacca      120

gatggaacta ttaaacgcct gatctacgcc gcatccactt tagattctgg tgtcccaaaa      180

aggttcagtg gcagtgagtc tgggtcagat tattctctca ccatcagcag tcttgagtct      240

gaagattttg cagactatta ctgtctacaa tattttagtt atccgctcac gttcggtgct      300

gggaccaagc tggagctgaa aggaggcgga tccggaggcg gaggccaggt ccaactgcag      360

cagcctgggg ctgagctggt gaggcctggg gcttcagtga agctgtcctg caaggcttct      420

ggctacacct tcaccagcta ctggatgaac tgggtgaagc agaggcctgg acaaggcctt      480

gaatggattg gtatggttga tccttcagac agtgaaactc actacaatca aatgttcaag      540

gacaaggcca cattgactgt tgacaaatcc tccagcacag cctacatgca gctcagcagc      600

ctgacatctg aggactctgc ggtctattac tgtgcaagag ctatgggcta ctggggtcaa      660

ggaacctcag tcaccgtctc ctcagttgag cccaaatctt gt                         702


<210>  231
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  8B5-CB3.1-VEPKSC

<400>  231

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Leu Ala Ala Leu Gly 
1               5                   10                  15      


Glu Arg Val Ser Leu Thr Cys Arg Ala Ser Gln Glu Ile Ser Gly Tyr 
            20                  25                  30          


Leu Ser Trp Leu Gln Gln Lys Pro Asp Gly Thr Ile Lys Arg Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Thr Leu Asp Ser Gly Val Pro Lys Arg Phe Ser Gly 
    50                  55                  60                  


Ser Glu Ser Gly Ser Asp Tyr Ser Leu Thr Ile Ser Ser Leu Glu Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Asp Tyr Tyr Cys Leu Gln Tyr Phe Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Arg 
        115                 120                 125             


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
    130                 135                 140                 


Thr Ser Tyr Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
145                 150                 155                 160 


Glu Trp Ile Gly Met Val Asp Pro Ser Asp Ser Glu Thr His Tyr Asn 
                165                 170                 175     


Gln Met Phe Lys Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser 
            180                 185                 190         


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
        195                 200                 205             


Tyr Tyr Cys Ala Arg Ala Met Gly Tyr Trp Gly Gln Gly Thr Ser Val 
    210                 215                 220                 


Thr Val Ser Ser Val Glu Pro Lys Ser Cys 
225                 230                 


<210>  232
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CB3.1-8B5-FNRGEC

<400>  232
gatgttgtga tgacccagac tccactcact ttgtcggtta acattggaca accagcctcc       60

atctcttgta agtcaagtca gagcctctta gatactgatg gaaagacata tttgaattgg      120

ttgttacaga ggccaggcca gtctccaaac cgcctaatct atctggtgtc taaactggac      180

tctggagtcc ctgacaggtt cactggcagt ggatcaggga cagatttcac actgaaaatc      240

agcagagtgg aggctgagga tttgggaatt tattattgct ggcaaggtac acattttccg      300

ctcacgttcg gtgctgggac caagctggag ctgaaaggag gcggatccgg aggcggaggc      360

gaagtgaagc ttgaggagtc tggaggaggc ttggtgcaac ctggaggatc catgaaactc      420

tcttgtgaag cctctggatt cacttttagt gacgcctgga tggactgggt ccgtcagtct      480

ccagagaagg ggcttgagtg ggttgctgaa attagaaaca aagctaaaaa tcatgcaaca      540

tactatgctg agtctgtgat agggaggttc accatctcaa gagatgattc caaaagtagt      600

gtctacctgc aaatgaacag cttaagagct gaagacactg gcatttatta ctgtggggct      660

ctgggccttg actactgggg ccaaggcacc actctcacag tctcctcgtt caacagggga      720

gagtgt                                                                 726


<210>  233
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CB3.1-8B5-FNRGEC

<400>  233

Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser Val Asn Ile Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Thr 
            20                  25                  30          


Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Asn Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Ile Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Glu Val Lys Leu Glu Glu Ser Gly 
        115                 120                 125             


Gly Gly Leu Val Gln Pro Gly Gly Ser Met Lys Leu Ser Cys Glu Ala 
    130                 135                 140                 


Ser Gly Phe Thr Phe Ser Asp Ala Trp Met Asp Trp Val Arg Gln Ser 
145                 150                 155                 160 


Pro Glu Lys Gly Leu Glu Trp Val Ala Glu Ile Arg Asn Lys Ala Lys 
                165                 170                 175     


Asn His Ala Thr Tyr Tyr Ala Glu Ser Val Ile Gly Arg Phe Thr Ile 
            180                 185                 190         


Ser Arg Asp Asp Ser Lys Ser Ser Val Tyr Leu Gln Met Asn Ser Leu 
        195                 200                 205             


Arg Ala Glu Asp Thr Gly Ile Tyr Tyr Cys Gly Ala Leu Gly Leu Asp 
    210                 215                 220                 


Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Phe Asn Arg Gly 
225                 230                 235                 240 


Glu Cys 
        


<210>  234
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  8B5VL-CB3.1VH-LGGC

<400>  234
gacattcaga tgacacagtc tccatcctcc ctacttgcgg cgctgggaga aagagtcagt       60

ctcacttgtc gggcaagtca ggaaattagt ggttacttaa gctggcttca gcagaaacca      120

gatggaacta ttaaacgcct gatctacgcc gcatccactt tagattctgg tgtcccaaaa      180

aggttcagtg gcagtgagtc tgggtcagat tattctctca ccatcagcag tcttgagtct      240

gaagattttg cagactatta ctgtctacaa tattttagtt atccgctcac gttcggtgct      300

gggaccaagc tggagctgaa aggaggcgga tccggaggcg gaggccaggt ccaactgcag      360

cagcctgggg ctgagctggt gaggcctggg gcttcagtga agctgtcctg caaggcttct      420

ggctacacct tcaccagcta ctggatgaac tgggtgaagc agaggcctgg acaaggcctt      480

gaatggattg gtatggttga tccttcagac agtgaaactc actacaatca aatgttcaag      540

gacaaggcca cattgactgt tgacaaatcc tccagcacag cctacatgca gctcagcagc      600

ctgacatctg aggactctgc ggtctattac tgtgcaagag ctatgggcta ctggggtcaa      660

ggaacctcag tcaccgtctc ctcactggga ggctgc                                696


<210>  235
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  8B5VL-CB3.1VH-LGGC

<400>  235

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Leu Ala Ala Leu Gly 
1               5                   10                  15      


Glu Arg Val Ser Leu Thr Cys Arg Ala Ser Gln Glu Ile Ser Gly Tyr 
            20                  25                  30          


Leu Ser Trp Leu Gln Gln Lys Pro Asp Gly Thr Ile Lys Arg Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Thr Leu Asp Ser Gly Val Pro Lys Arg Phe Ser Gly 
    50                  55                  60                  


Ser Glu Ser Gly Ser Asp Tyr Ser Leu Thr Ile Ser Ser Leu Glu Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Asp Tyr Tyr Cys Leu Gln Tyr Phe Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Arg 
        115                 120                 125             


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
    130                 135                 140                 


Thr Ser Tyr Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
145                 150                 155                 160 


Glu Trp Ile Gly Met Val Asp Pro Ser Asp Ser Glu Thr His Tyr Asn 
                165                 170                 175     


Gln Met Phe Lys Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser 
            180                 185                 190         


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
        195                 200                 205             


Tyr Tyr Cys Ala Arg Ala Met Gly Tyr Trp Gly Gln Gly Thr Ser Val 
    210                 215                 220                 


Thr Val Ser Ser Leu Gly Gly Cys 
225                 230         


<210>  236
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CB3.1-8B5-LGGC

<400>  236
gatgttgtga tgacccagac tccactcact ttgtcggtta acattggaca accagcctcc       60

atctcttgta agtcaagtca gagcctctta gatactgatg gaaagacata tttgaattgg      120

ttgttacaga ggccaggcca gtctccaaac cgcctaatct atctggtgtc taaactggac      180

tctggagtcc ctgacaggtt cactggcagt ggatcaggga cagatttcac actgaaaatc      240

agcagagtgg aggctgagga tttgggaatt tattattgct ggcaaggtac acattttccg      300

ctcacgttcg gtgctgggac caagctggag ctgaaaggag gcggatccgg aggcggaggc      360

gaagtgaagc ttgaggagtc tggaggaggc ttggtgcaac ctggaggatc catgaaactc      420

tcttgtgaag cctctggatt cacttttagt gacgcctgga tggactgggt ccgtcagtct      480

ccagagaagg ggcttgagtg ggttgctgaa attagaaaca aagctaaaaa tcatgcaaca      540

tactatgctg agtctgtgat agggaggttc accatctcaa gagatgattc caaaagtagt      600

gtctacctgc aaatgaacag cttaagagct gaagacactg gcatttatta ctgtggggct      660

ctgggccttg actactgggg ccaaggcacc actctcacag tctcctcgct gggaggctgc      720


<210>  237
<211>  240
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CB3.1-8B5-LGGC

<400>  237

Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser Val Asn Ile Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Thr 
            20                  25                  30          


Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Asn Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Ile Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Glu Val Lys Leu Glu Glu Ser Gly 
        115                 120                 125             


Gly Gly Leu Val Gln Pro Gly Gly Ser Met Lys Leu Ser Cys Glu Ala 
    130                 135                 140                 


Ser Gly Phe Thr Phe Ser Asp Ala Trp Met Asp Trp Val Arg Gln Ser 
145                 150                 155                 160 


Pro Glu Lys Gly Leu Glu Trp Val Ala Glu Ile Arg Asn Lys Ala Lys 
                165                 170                 175     


Asn His Ala Thr Tyr Tyr Ala Glu Ser Val Ile Gly Arg Phe Thr Ile 
            180                 185                 190         


Ser Arg Asp Asp Ser Lys Ser Ser Val Tyr Leu Gln Met Asn Ser Leu 
        195                 200                 205             


Arg Ala Glu Asp Thr Gly Ile Tyr Tyr Cys Gly Ala Leu Gly Leu Asp 
    210                 215                 220                 


Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Leu Gly Gly Cys 
225                 230                 235                 240 


<210>  238
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh628F Primer

<400>  238
ggaggcggat ccggaggcgg aggccaggtc caactgcagc agcctgg                     47


<210>  239
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh629R Primer

<400>  239
tttgaattct aacaagattt gggctcaact gaggagacgg tgactgagg                   49


<210>  240
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh630R Primer

<400>  240
gcctccgcct ccggatccgc ctcctttcag ctccagcttg gtccc                       45


<210>  241
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh631F Primer

<400>  241
ggaggcggat ccggaggcgg aggcgaagtg aagcttgagg agtctgg                     47


<210>  242
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh640R Primer

<400>  242
tttgaattct aacactctcc cctgttgaac gaggagactg tgagagtgg                   49


<210>  243
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh644R Primer

<400>  243
tttgtcgtca tcatcgtctt tgtagtcgga gtggacacct gtggagag                    48


<210>  244
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh646R Primer

<400>  244
tttgaattct agcagcctcc cagtgaggag acggtgactg ag                          42


<210>  245
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh647F Primer

<400>  245
caaagacgat gatgacgaca aagacattca gatgacacag tctcc                       45


<210>  246
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh648R Primer

<400>  246
tttgaattct agcagcctcc cagcgaggag actgtgagag tgg                         43


<210>  247
<211>  1044
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2.4G2-3G8-hKappa

<400>  247
gatgtccaga tgacccagtc tccatctaat cttgctgcct ctcctggaga aagtgtttcc       60

atcaattgca aggcaagtga gagcattagc aagtatttag cctggtatct acagaaacct      120

gggaaagcaa ataagcttct tatgtacgat gggtcaactt tgcaatctgg aattccatcg      180

aggttcagtg gcagtggatc tggtacagat ttcactctca ccatcagaag cctggagcct      240

gaagattttg gactctatta ctgtcaacag cattatgaat atccagccac gttcggttct      300

gggaccaagc tggagatcaa aggaggcgga tccggaggcg gaggccaggt taccctgaaa      360

gagtctggcc ctgggatatt gcagccctcc cagaccctca gtctgacttg ttctttctct      420

gggttttcac tgaggacttc tggtatgggt gtaggctgga ttcgtcagcc ttcagggaag      480

ggtctagagt ggctggcaca catttggtgg gatgatgaca agcgctataa tccagccctg      540

aagagccgac tgacaatctc caaggatacc tccagcaacc aggtattcct caaaatcgcc      600

agtgtggaca ctgcagatac tgccacatac tactgtgctc aaataaaccc cgcctggttt      660

gcttactggg gccaagggac tctggtcact gtgagctcac tgggaggctg cggcggaggg      720

agccgtacgg tggctgcacc atcggtcttc atcttcccgc catctgatga gcagttgaaa      780

tctggaactg cctctgttgt gtgcctgctg aataacttct atcccagaga ggccaaagta      840

cagtggaagg tggataacgc cctccaatcg ggtaactccc aggagagtgt cacagagcag      900

gacagcaagg acagcaccta cagcctcagc agcaccctga cgctgagcaa agcagactac      960

gagaaacaca aagtctacgc ctgcgaagtc acccatcagg gcctgagctc gcccgtcaca     1020

aagagcttca acaggggaga gtgt                                            1044


<210>  248
<211>  348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2.4G2-3G8-hKappa

<400>  248

Asp Val Gln Met Thr Gln Ser Pro Ser Asn Leu Ala Ala Ser Pro Gly 
1               5                   10                  15      


Glu Ser Val Ser Ile Asn Cys Lys Ala Ser Glu Ser Ile Ser Lys Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Leu Gln Lys Pro Gly Lys Ala Asn Lys Leu Leu Met 
        35                  40                  45              


Tyr Asp Gly Ser Thr Leu Gln Ser Gly Ile Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Arg Ser Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Gly Leu Tyr Tyr Cys Gln Gln His Tyr Glu Tyr Pro Ala 
                85                  90                  95      


Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Thr Leu Lys Glu Ser Gly Pro Gly Ile Leu Gln 
        115                 120                 125             


Pro Ser Gln Thr Leu Ser Leu Thr Cys Ser Phe Ser Gly Phe Ser Leu 
    130                 135                 140                 


Arg Thr Ser Gly Met Gly Val Gly Trp Ile Arg Gln Pro Ser Gly Lys 
145                 150                 155                 160 


Gly Leu Glu Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr 
                165                 170                 175     


Asn Pro Ala Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Ser 
            180                 185                 190         


Asn Gln Val Phe Leu Lys Ile Ala Ser Val Asp Thr Ala Asp Thr Ala 
        195                 200                 205             


Thr Tyr Tyr Cys Ala Gln Ile Asn Pro Ala Trp Phe Ala Tyr Trp Gly 
    210                 215                 220                 


Gln Gly Thr Leu Val Thr Val Ser Ser Leu Gly Gly Cys Gly Gly Gly 
225                 230                 235                 240 


Ser Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp 
                245                 250                 255     


Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn 
            260                 265                 270         


Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu 
        275                 280                 285             


Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp 
    290                 295                 300                 


Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr 
305                 310                 315                 320 


Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser 
                325                 330                 335     


Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            340                 345             


<210>  249
<211>  1734
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3G8-2.4G2-hG1

<400>  249
gacactgtgc tgacccaatc tccagcttct ttggctgtgt ctctagggca gagggccacc       60

atctcctgca aggccagcca aagtgttgat tttgatggtg atagttttat gaactggtac      120

caacagaaac caggacagcc acccaaactc ctcatctata ctacatccaa tctagaatct      180

gggatcccag ccaggtttag tgccagtggg tctgggacag acttcaccct caacatccat      240

cctgtggagg aggaggatac tgcaacctat tactgtcagc aaagtaatga ggatccgtac      300

acgttcggag gggggaccaa gctggaaata aaaggaggcg gatccggagg cggaggcgag      360

gtggagctag tggagtctgg gggaggctta gtgcagcctg gaaggtccct gaaactctcg      420

tgtgcagcct caggattcac tttcagtgac tattacatgg cctgggtccg gcaggctcca      480

acgacgggtc tggagtgggt cgcatccatt agttatgatg gtggtgacac tcactatcga      540

gactccgtga agggccgatt tactatttcc agagataatg caaaaagcag cctatacctg      600

caaatggaca gtctgaggtc tgaggacacg gccacttatt actgtgcaac agagactacg      660

ggaataccta caggtgttat ggatgcctgg ggtcaaggag tttcagtcac tgtctcctca      720

ctgggaggct gcggcggagg gagcgcctcc accaagggcc catcggtctt ccccctggca      780

ccctcctcca agagcacctc tgggggcaca gcggccctgg gctgcctggt caaggactac      840

ttccccgaac cggtgacggt gtcgtggaac tcaggcgccc tgaccagcgg cgtgcacacc      900

ttcccggctg tcctacagtc ctcaggactc tactccctca gcagcgtggt gaccgtgccc      960

tccagcagct tgggcaccca gacctacatc tgcaacgtga atcacaagcc cagcaacacc     1020

aaggtggaca agagagttga gcccaaatct tgtgacaaaa ctcacacatg cccaccgtgc     1080

ccagcacctg aactcctggg gggaccgtca gtcttcctct tccccccaaa acccaaggac     1140

accctcatga tctcccggac ccctgaggtc acatgcgtgg tggtggacgt gagccacgaa     1200

gaccctgagg tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa tgccaagaca     1260

aagccgcggg aggagcagta caacagcacg taccgtgtgg tcagcgtcct caccgtcctg     1320

caccaggact ggctgaatgg caaggagtac aagtgcaagg tctccaacaa agccctccca     1380

gcccccatcg agaaaaccat ctccaaagcc aaagggcagc cccgagaacc acaggtgtac     1440

accctgcccc catcccggga tgagctgacc aagaaccagg tcagcctgac ctgcctggtc     1500

aaaggcttct atcccagcga catcgccgtg gagtgggaga gcaatgggca gccggagaac     1560

aactacaaga ccacgcctcc cgtgctggac tccgacggct ccttcttcct ctacagcaag     1620

ctcaccgtgg acaagagcag gtggcagcag gggaacgtct tctcatgctc cgtgatgcat     1680

gaggctctgc acaaccacta cacgcagaag agcctctccc tgtctccggg taaa           1734


<210>  250
<211>  578
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3G8-2.4G2-hG1

<400>  250

Asp Thr Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Ala Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Glu Asp Thr Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Glu Val Glu Leu Val Glu Ser Gly Gly 
        115                 120                 125             


Gly Leu Val Gln Pro Gly Arg Ser Leu Lys Leu Ser Cys Ala Ala Ser 
    130                 135                 140                 


Gly Phe Thr Phe Ser Asp Tyr Tyr Met Ala Trp Val Arg Gln Ala Pro 
145                 150                 155                 160 


Thr Thr Gly Leu Glu Trp Val Ala Ser Ile Ser Tyr Asp Gly Gly Asp 
                165                 170                 175     


Thr His Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
            180                 185                 190         


Asn Ala Lys Ser Ser Leu Tyr Leu Gln Met Asp Ser Leu Arg Ser Glu 
        195                 200                 205             


Asp Thr Ala Thr Tyr Tyr Cys Ala Thr Glu Thr Thr Gly Ile Pro Thr 
    210                 215                 220                 


Gly Val Met Asp Ala Trp Gly Gln Gly Val Ser Val Thr Val Ser Ser 
225                 230                 235                 240 


Leu Gly Gly Cys Gly Gly Gly Ser Ala Ser Thr Lys Gly Pro Ser Val 
                245                 250                 255     


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
            260                 265                 270         


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
        275                 280                 285             


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
    290                 295                 300                 


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
305                 310                 315                 320 


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
                325                 330                 335     


Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp 
            340                 345                 350         


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
        355                 360                 365             


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
    370                 375                 380                 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
385                 390                 395                 400 


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
                405                 410                 415     


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
            420                 425                 430         


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
        435                 440                 445             


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
    450                 455                 460                 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
465                 470                 475                 480 


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
                485                 490                 495     


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
            500                 505                 510         


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
        515                 520                 525             


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
    530                 535                 540                 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
545                 550                 555                 560 


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
                565                 570                 575     


Gly Lys 
        


<210>  251
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD79VL-CD32BVH

<400>  251
gatgttgtga tgactcagtc tccactctcc ctgcccgtca cccttggaca gccggcctcc       60

atctcctgca agtcaagtca gagcctctta gatagtgatg gaaagacata tttgaattgg      120

tttcagcaga ggccaggcca atctccaaac cgcctaattt atctggtgtc taaactggac      180

tctggggtcc cagacagatt cagcggcagt gggtcaggca ctgatttcac actgaaaatc      240

agcagggtgg aggctgagga tgttggggtt tattactgct ggcaaggtac acattttccg      300

ctcacgttcg gcggagggac caagcttgag atcaaaggag gcggatccgg aggcggaggc      360

gaagtgaagc ttgaggagtc tggaggaggc ttggtgcaac ctggaggatc catgaaactc      420

tcttgtgaag cctctggatt cacttttagt gacgcctgga tggactgggt ccgtcagtct      480

ccagagaagg ggcttgagtg ggttgctgaa attagaaaca aagctaaaaa tcatgcaaca      540

tactatgctg agtctgtgat agggaggttc accatctcaa gagatgattc caaaagtagt      600

gtctacctgc aaatgaacag cttaagagct gaagacactg gcatttatta ctgtggggct      660

ctgggccttg actactgggg ccaaggcacc actctcacag tctcctcgct gggaggctgc      720


<210>  252
<211>  240
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD79VL-CD32BVH

<400>  252

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Ser 
            20                  25                  30          


Asp Gly Lys Thr Tyr Leu Asn Trp Phe Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Asn Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Glu Val Gln Leu Val Glu Ser Gly 
        115                 120                 125             


Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala 
    130                 135                 140                 


Ser Gly Phe Thr Phe Ser Asp Ala Trp Met Asp Trp Val Arg Gln Ala 
145                 150                 155                 160 


Pro Gly Lys Gly Leu Glu Trp Val Ala Glu Ile Arg Asn Lys Ala Lys 
                165                 170                 175     


Asn His Ala Thr Tyr Tyr Ala Glu Ser Val Ile Gly Arg Phe Thr Ile 
            180                 185                 190         


Ser Arg Asp Asp Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu 
        195                 200                 205             


Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Gly Ala Leu Gly Leu Asp 
    210                 215                 220                 


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Leu Gly Gly Cys 
225                 230                 235                 240 


<210>  253
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD32BVL-CD79VH-1

<400>  253
gacatccaga tgacccagtc tccatcctcc ttatctgcct ctgtgggaga tagagtcacc       60

atcacttgtc gggcaagtca ggaaattagt ggttacttaa gctggctgca gcagaaacca      120

ggcaaggccc ctagacgcct gatctacgcc gcatccactt tagattctgg tgtcccatcc      180

aggttcagtg gcagtgagtc tgggaccgag ttcaccctca ccatcagcag ccttcagcct      240

gaagattttg caacctatta ctgtctacaa tattttagtt atccgctcac gttcggaggg      300

gggaccaagg tggaaataaa aggaggcgga tccggaggcg gaggccaggt tcagctggtg      360

cagtctggag ctgaggtgaa gaagcctggc gcctcagtga aggtctcctg caaggcttct      420

ggttacacct ttaccagcta ctggatgaac tgggtgcgac aggcccctgg acaagggctt      480

gagtggatcg gaatgattga tccttcagac agtgaaactc actacaatca aatgttcaag      540

gacagagtca ccatgaccac agacacatcc acgagcacag cctacatgga gctgaggagc      600

ctgagatctg acgacacggc cgtgtattac tgtgcgagag ctatgggcta ctgggggcaa      660

gggaccacgg tcaccgtctc ctcactggga ggctgc                                696


<210>  254
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD32BVL-CD79VH-1

<400>  254

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Glu Ile Ser Gly Tyr 
            20                  25                  30          


Leu Ser Trp Leu Gln Gln Lys Pro Gly Lys Ala Pro Arg Arg Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Thr Leu Asp Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Glu Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Tyr Phe Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
        115                 120                 125             


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
    130                 135                 140                 


Thr Ser Tyr Trp Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
145                 150                 155                 160 


Glu Trp Ile Gly Met Ile Asp Pro Ser Asp Ser Glu Thr His Tyr Asn 
                165                 170                 175     


Gln Met Phe Lys Asp Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser 
            180                 185                 190         


Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val 
        195                 200                 205             


Tyr Tyr Cys Ala Arg Ala Met Gly Tyr Trp Gly Gln Gly Thr Thr Val 
    210                 215                 220                 


Thr Val Ser Ser Leu Gly Gly Cys 
225                 230         


<210>  255
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD32BVL-CD79VH-2

<400>  255
gacatccaga tgacccagtc tccatcctcc ttatctgcct ctgtgggaga tagagtcacc       60

atcacttgtc gggcaagtca ggaaattagt ggttacttaa gctggctgca gcagaaacca      120

ggcaaggccc ctagacgcct gatctacgcc gcatccactt tagattctgg tgtcccatcc      180

aggttcagtg gcagtgagtc tgggaccgag ttcaccctca ccatcagcag ccttcagcct      240

gaagattttg caacctatta ctgtctacaa tattttagtt atccgctcac gttcggaggg      300

gggaccaagg tggaaataaa aggaggcgga tccggaggcg gaggccaggt tcagctggtg      360

cagtctggag ctgaggtgaa gaagcctggc gcctcagtga aggtctcctg caaggcttct      420

ggttacacct ttaccagcta ctggatgaac tgggtgcgac aggcccctgg acaagggctt      480

gagtggatcg gaatgattga tccttcagac agtgaaactc actacaatca aaagttcaag      540

gacagagtca ccatgaccac agacacatcc acgagcacag cctacatgga gctgaggagc      600

ctgagatctg acgacacggc cgtgtattac tgtgcgagag ctatgggcta ctgggggcaa      660

gggaccacgg tcaccgtctc ctcactggga ggctgc                                696


<210>  256
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD32BVL-CD79VH-2

<400>  256

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Glu Ile Ser Gly Tyr 
            20                  25                  30          


Leu Ser Trp Leu Gln Gln Lys Pro Gly Lys Ala Pro Arg Arg Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Thr Leu Asp Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Glu Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Tyr Phe Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
        115                 120                 125             


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
    130                 135                 140                 


Thr Ser Tyr Trp Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
145                 150                 155                 160 


Glu Trp Ile Gly Met Ile Asp Pro Ser Asp Ser Glu Thr His Tyr Asn 
                165                 170                 175     


Gln Lys Phe Lys Asp Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser 
            180                 185                 190         


Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val 
        195                 200                 205             


Tyr Tyr Cys Ala Arg Ala Met Gly Tyr Trp Gly Gln Gly Thr Thr Val 
    210                 215                 220                 


Thr Val Ser Ser Leu Gly Gly Cys 
225                 230         


<210>  257
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hHBCRCVL. R45N-h8B5VH-LGGC

<400>  257
gatgttgtga tgactcagtc tccactctcc ctgcccgtca cccttggaca gccggcctcc       60

atctcctgca agtcaagtca gagcctctta gatagtgatg gaaagacata tttgaattgg      120

tttcagcaga ggccaggcca atctccaaac cgcctaattt atctggtgtc taaactggac      180

tctggggtcc cagacagatt cagcggcagt gggtcaggca ctgatttcac actgaaaatc      240

agcagggtgg aggctgagga tgttggggtt tattactgct ggcaaggtac acattttccg      300

ctcacgttcg gcggagggac caagcttgag atcaaaggag gcggatccgg aggcggaggc      360

gaagtgaagc ttgaggagtc tggaggaggc ttggtgcaac ctggaggatc catgaaactc      420

tcttgtgaag cctctggatt cacttttagt gacgcctgga tggactgggt ccgtcagtct      480

ccagagaagg ggcttgagtg ggttgctgaa attagaaaca aagctaaaaa tcatgcaaca      540

tactatgctg agtctgtgat agggaggttc accatctcaa gagatgattc caaaagtagt      600

gtctacctgc aaatgaacag cttaagagct gaagacactg gcatttatta ctgtggggct      660

ctgggccttg actactgggg ccaaggcacc actctcacag tctcctcgct gggaggctgc      720


<210>  258
<211>  240
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hHBCRCVL. R45N-h8B5VH-LGGC

<400>  258

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Ser 
            20                  25                  30          


Asp Gly Lys Thr Tyr Leu Asn Trp Phe Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Asn Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Glu Val Gln Leu Val Glu Ser Gly 
        115                 120                 125             


Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala 
    130                 135                 140                 


Ser Gly Phe Thr Phe Ser Asp Ala Trp Met Asp Trp Val Arg Gln Ala 
145                 150                 155                 160 


Pro Gly Lys Gly Leu Glu Trp Val Ala Glu Ile Arg Asn Lys Ala Lys 
                165                 170                 175     


Asn His Ala Thr Tyr Tyr Ala Glu Ser Val Ile Gly Arg Phe Thr Ile 
            180                 185                 190         


Ser Arg Asp Asp Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu 
        195                 200                 205             


Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Gly Ala Leu Gly Leu Asp 
    210                 215                 220                 


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Leu Gly Gly Cys 
225                 230                 235                 240 


<210>  259
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H8B5VL-hHBCRCVH M48I-LGGC

<400>  259
gacatccaga tgacccagtc tccatcctcc ttatctgcct ctgtgggaga tagagtcacc       60

atcacttgtc gggcaagtca ggaaattagt ggttacttaa gctggctgca gcagaaacca      120

ggcaaggccc ctagacgcct gatctacgcc gcatccactt tagattctgg tgtcccatcc      180

aggttcagtg gcagtgagtc tgggaccgag ttcaccctca ccatcagcag ccttcagcct      240

gaagattttg caacctatta ctgtctacaa tattttagtt atccgctcac gttcggaggg      300

gggaccaagg tggaaataaa aggaggcgga tccggaggcg gaggccaggt tcagctggtg      360

cagtctggag ctgaggtgaa gaagcctggc gcctcagtga aggtctcctg caaggcttct      420

ggttacacct ttaccagcta ctggatgaac tgggtgcgac aggcccctgg acaagggctt      480

gagtggatcg gaatgattga tccttcagac agtgaaactc actacaatca aatgttcaag      540

gacagagtca ccatgaccac agacacatcc acgagcacag cctacatgga gctgaggagc      600

ctgagatctg acgacacggc cgtgtattac tgtgcgagag ctatgggcta ctgggggcaa      660

gggaccacgg tcaccgtctc ctcactggga ggctgc                                696


<210>  260
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H8B5VL-HBCRCVH M48I-LGGC

<400>  260

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Glu Ile Ser Gly Tyr 
            20                  25                  30          


Leu Ser Trp Leu Gln Gln Lys Pro Gly Lys Ala Pro Arg Arg Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Thr Leu Asp Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Glu Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Tyr Phe Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
        115                 120                 125             


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
    130                 135                 140                 


Thr Ser Tyr Trp Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
145                 150                 155                 160 


Glu Trp Ile Gly Met Ile Asp Pro Ser Asp Ser Glu Thr His Tyr Asn 
                165                 170                 175     


Gln Met Phe Lys Asp Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser 
            180                 185                 190         


Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val 
        195                 200                 205             


Tyr Tyr Cys Ala Arg Ala Met Gly Tyr Trp Gly Gln Gly Thr Thr Val 
    210                 215                 220                 


Thr Val Ser Ser Leu Gly Gly Cys 
225                 230         


<210>  261
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh321F Primer

<400>  261
cgagctagct ctagatgaga tcacagttct ctctac                                 36


<210>  262
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh386R Primer

<400>  262
tttgaattct agcagcctcc cagtgaggag acggtgaccg tggtc                       45


<210>  263
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh784F Primer

<400>  263
ggcggatccg gaggcggagg ccaggttcag ctggtgcag                              39


<210>  264
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh785R Primer

<400>  264
cctccggatc cgcctccttt gatctcaagc ttggtccc                               38


<210>  265
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh786R Primer

<400>  265
tttgaattct agcagcctcc caggctggag acggtcacca gg                          42


<210>  266
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lgh787F Primer

<400>  266
ggaggcggat ccggaggcgg aggcgaagtg cagcttgtgg agtc                        44


<210>  267
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  267

Leu Gly Gly Cys Gly Gly Gly Ser 
1               5               


<210>  268
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  268

Leu Glu Ile Lys 
1               


<210>  269
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  269

Thr Val Ser Ser 
1               


<210>  270
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H8B5VL-hBCRCVH M48I, M62K_LGGCG3S_hKappa

<400>  270

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Glu Ile Ser Gly Tyr 
            20                  25                  30          


Leu Ser Trp Leu Gln Gln Lys Pro Gly Lys Ala Pro Arg Arg Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Thr Leu Asp Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Glu Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Tyr Phe Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
        115                 120                 125             


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
    130                 135                 140                 


Thr Ser Tyr Trp Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
145                 150                 155                 160 


Glu Trp Ile Gly Met Ile Asp Pro Ser Asp Ser Glu Thr His Tyr Asn 
                165                 170                 175     


Gln Lys Phe Lys Asp Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser 
            180                 185                 190         


Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val 
        195                 200                 205             


Tyr Tyr Cys Ala Arg Ala Met Gly Tyr Trp Gly Gln Gly Thr Thr Val 
    210                 215                 220                 


Thr Val Ser Ser Leu Gly Gly Cys Gly Gly Gly Ser Arg Thr Val Ala 
225                 230                 235                 240 


Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser 
                245                 250                 255     


Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu 
            260                 265                 270         


Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser 
        275                 280                 285             


Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu 
    290                 295                 300                 


Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val 
305                 310                 315                 320 


Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys 
                325                 330                 335     


Ser Phe Asn Arg Gly Glu Cys 
            340             


<210>  271
<211>  1032
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H8B5VL-hBCRCVH M48I, M62K_LGGCG3S_hKappa

<400>  271
gacatccaga tgacccagtc tccatcctcc ttatctgcct ctgtgggaga tagagtcacc       60

atcacttgtc gggcaagtca ggaaattagt ggttacttaa gctggctgca gcagaaacca      120

ggcaaggccc ctagacgcct gatctacgcc gcatccactt tagattctgg tgtcccatcc      180

aggttcagtg gcagtgagtc tgggaccgag ttcaccctca ccatcagcag ccttcagcct      240

gaagattttg caacctatta ctgtctacaa tattttagtt atccgctcac gttcggaggg      300

gggaccaagg tggaaataaa aggaggcgga tccggaggcg gaggccaggt tcagctggtg      360

cagtctggag ctgaggtgaa gaagcctggc gcctcagtga aggtctcctg caaggcttct      420

ggttacacct ttaccagcta ctggatgaac tgggtgcgac aggcccctgg acaagggctt      480

gagtggatcg gaatgattga tccttcagac agtgaaactc actacaatca aaagttcaag      540

gacagagtca ccatgaccac agacacatcc acgagcacag cctacatgga gctgaggagc      600

ctgagatctg acgacacggc cgtgtattac tgtgcgagag ctatgggcta ctgggggcaa      660

gggaccacgg tcaccgtctc ctcactggga ggctgcggcg gagggagccg aactgtggct      720

gcaccatcgg tcttcatctt cccgccatct gatgagcagt tgaaatctgg aactgcctct      780

gttgtgtgcc tgctgaataa cttctatccc agagaggcca aagtacagtg gaaggtggat      840

aacgccctcc aatcgggtaa ctcccaggag agtgtcacag agcaggacag caaggacagc      900

acctacagcc tcagcagcac cctgacgctg agcaaagcag actacgagaa acacaaagtc      960

tacgcctgcg aagtcaccca tcagggcctg agctcgcccg tcacaaagag cttcaacagg     1020

ggagagtgtt ag                                                         1032


<210>  272
<211>  574
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HBCRCVL R45N-h8B5VH_LGGCGGGS-hG1

<400>  272

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Ser 
            20                  25                  30          


Asp Gly Lys Thr Tyr Leu Asn Trp Phe Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Asn Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Glu Val Gln Leu Val Glu Ser Gly 
        115                 120                 125             


Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala 
    130                 135                 140                 


Ser Gly Phe Thr Phe Ser Asp Ala Trp Met Asp Trp Val Arg Gln Ala 
145                 150                 155                 160 


Pro Gly Lys Gly Leu Glu Trp Val Ala Glu Ile Arg Asn Lys Ala Lys 
                165                 170                 175     


Asn His Ala Thr Tyr Tyr Ala Glu Ser Val Ile Gly Arg Phe Thr Ile 
            180                 185                 190         


Ser Arg Asp Asp Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu 
        195                 200                 205             


Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Gly Ala Leu Gly Leu Asp 
    210                 215                 220                 


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Leu Gly Gly Cys 
225                 230                 235                 240 


Gly Gly Gly Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 
                245                 250                 255     


Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 
            260                 265                 270         


Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 
        275                 280                 285             


Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 
    290                 295                 300                 


Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 
305                 310                 315                 320 


Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 
                325                 330                 335     


Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
            340                 345                 350         


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
        355                 360                 365             


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
    370                 375                 380                 


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
385                 390                 395                 400 


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
                405                 410                 415     


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
            420                 425                 430         


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
        435                 440                 445             


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
    450                 455                 460                 


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
465                 470                 475                 480 


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
                485                 490                 495     


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
            500                 505                 510         


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
        515                 520                 525             


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
    530                 535                 540                 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
545                 550                 555                 560 


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                565                 570                 


<210>  273
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBCRCVL R45N-h8B5VH_LGGCGGGS-hG1

<400>  273
gatgttgtga tgactcagtc tccactctcc ctgcccgtca cccttggaca gccggcctcc       60

atctcctgca agtcaagtca gagcctctta gatagtgatg gaaagacata tttgaattgg      120

tttcagcaga ggccaggcca atctccaaac cgcctaattt atctggtgtc taaactggac      180

tctggggtcc cagacagatt cagcggcagt gggtcaggca ctgatttcac actgaaaatc      240

agcagggtgg aggctgagga tgttggggtt tattactgct ggcaaggtac acattttccg      300

ctcacgttcg gcggagggac caagcttgag atcaaaggag gcggatccgg aggcggaggc      360

gaagtgcagc ttgtggagtc tggaggaggc ttggtgcaac ctggaggatc cctgagactc      420

tcttgtgccg cctctggatt cacttttagt gacgcctgga tggactgggt ccgtcaggcc      480

ccaggcaagg ggcttgagtg ggttgctgaa attagaaaca aagctaaaaa tcatgcaaca      540

tactatgctg agtctgtgat agggaggttc accatctcaa gagatgacgc caaaaacagt      600

ctgtacctgc aaatgaacag cttaagagct gaagacactg ccgtgtatta ctgtggggct      660

ctgggccttg actactgggg ccaaggcacc ctggtgaccg tctccagcct gggaggctgc      720

ggcggaggga gcgcctccac caagggccca tcggtcttcc ccctggcacc ctcctccaag      780

agcacctctg ggggcacagc ggccctgggc tgcctggtca aggactactt ccccgaaccg      840

gtgacggtgt cgtggaactc aggcgccctg accagcggcg tgcacacctt cccggctgtc      900

ctacagtcct caggactcta ctccctcagc agcgtggtga ccgtgccctc cagcagcttg      960

ggcacccaga cctacatctg caacgtgaat cacaagccca gcaacaccaa ggtggacaag     1020

agagttgagc ccaaatcttg tgacaaaact cacacatgcc caccgtgccc agcacctgaa     1080

ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac cctcatgatc     1140

tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc     1200

aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa gccgcgggag     1260

gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg     1320

ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag     1380

aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac cctgccccca     1440

tcccgggatg agctgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctat     1500

cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc     1560

acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct caccgtggac     1620

aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga ggctctgcac     1680

aaccactaca cgcagaagag cctctccctg tctccgggta aa                        1722


<210>  274
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H8B5VL-HBCRCVH M48I, M62K_(-4)LEIK_hKappa

<400>  274

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Glu Ile Ser Gly Tyr 
            20                  25                  30          


Leu Ser Trp Leu Gln Gln Lys Pro Gly Lys Ala Pro Arg Arg Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Thr Leu Asp Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Glu Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Tyr Phe Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
        115                 120                 125             


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
    130                 135                 140                 


Thr Ser Tyr Trp Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
145                 150                 155                 160 


Glu Trp Ile Gly Met Ile Asp Pro Ser Asp Ser Glu Thr His Tyr Asn 
                165                 170                 175     


Gln Lys Phe Lys Asp Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser 
            180                 185                 190         


Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val 
        195                 200                 205             


Tyr Tyr Cys Ala Arg Ala Met Gly Tyr Trp Gly Gln Gly Thr Thr Val 
    210                 215                 220                 


Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro 
225                 230                 235                 240 


Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu 
                245                 250                 255     


Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp 
            260                 265                 270         


Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp 
        275                 280                 285             


Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys 
    290                 295                 300                 


Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln 
305                 310                 315                 320 


Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
                325                 330                 335 


<210>  275
<211>  1005
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H8B5VL-HBCRCVH M48I, M62K_(-4)LEIK_hKappa

<400>  275
gacatccaga tgacccagtc tccatcctcc ttatctgcct ctgtgggaga tagagtcacc       60

atcacttgtc gggcaagtca ggaaattagt ggttacttaa gctggctgca gcagaaacca      120

ggcaaggccc ctagacgcct gatctacgcc gcatccactt tagattctgg tgtcccatcc      180

aggttcagtg gcagtgagtc tgggaccgag ttcaccctca ccatcagcag ccttcagcct      240

gaagattttg caacctatta ctgtctacaa tattttagtt atccgctcac gttcggaggg      300

gggaccaagg tggaaataaa aggaggcgga tccggaggcg gaggccaggt tcagctggtg      360

cagtctggag ctgaggtgaa gaagcctggc gcctcagtga aggtctcctg caaggcttct      420

ggttacacct ttaccagcta ctggatgaac tgggtgcgac aggcccctgg acaagggctt      480

gagtggatcg gaatgattga tccttcagac agtgaaactc actacaatca aaagttcaag      540

gacagagtca ccatgaccac agacacatcc acgagcacag cctacatgga gctgaggagc      600

ctgagatctg acgacacggc cgtgtattac tgtgcgagag ctatgggcta ctgggggcaa      660

gggaccacgg tcctggagat caagcgaact gtggctgcac catcggtctt catcttcccg      720

ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct gaataacttc      780

tatcccagag aggccaaagt acagtggaag gtggataacg ccctccaatc gggtaactcc      840

caggagagtg tcacagagca ggacagcaag gacagcacct acagcctcag cagcaccctg      900

acgctgagca aagcagacta cgagaaacac aaagtctacg cctgcgaagt cacccatcag      960

ggcctgagct cgcccgtcac aaagagcttc aacaggggag agtgt                     1005


<210>  276
<211>  566
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HBCRCVL R45N-h8B5VH_(-4)TVSS-hG1  = HBCRCVL R45N-h8B5VH_-hG1

<400>  276

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Ser 
            20                  25                  30          


Asp Gly Lys Thr Tyr Leu Asn Trp Phe Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Asn Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Glu Val Gln Leu Val Glu Ser Gly 
        115                 120                 125             


Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala 
    130                 135                 140                 


Ser Gly Phe Thr Phe Ser Asp Ala Trp Met Asp Trp Val Arg Gln Ala 
145                 150                 155                 160 


Pro Gly Lys Gly Leu Glu Trp Val Ala Glu Ile Arg Asn Lys Ala Lys 
                165                 170                 175     


Asn His Ala Thr Tyr Tyr Ala Glu Ser Val Ile Gly Arg Phe Thr Ile 
            180                 185                 190         


Ser Arg Asp Asp Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu 
        195                 200                 205             


Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Gly Ala Leu Gly Leu Asp 
    210                 215                 220                 


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
225                 230                 235                 240 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
                245                 250                 255     


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
            260                 265                 270         


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
        275                 280                 285             


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
    290                 295                 300                 


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
305                 310                 315                 320 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro 
                325                 330                 335     


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
            340                 345                 350         


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        355                 360                 365             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    370                 375                 380                 


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
385                 390                 395                 400 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
                405                 410                 415     


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
            420                 425                 430         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
        435                 440                 445             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
    450                 455                 460                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn 
465                 470                 475                 480 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                485                 490                 495     


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            500                 505                 510         


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        515                 520                 525             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    530                 535                 540                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
545                 550                 555                 560 


Ser Leu Ser Pro Gly Lys 
                565     


<210>  277
<211>  1698
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBCRCVL R45N-h8B5VH_(-4)TVSS-hG1

<400>  277
gatgttgtga tgactcagtc tccactctcc ctgcccgtca cccttggaca gccggcctcc       60

atctcctgca agtcaagtca gagcctctta gatagtgatg gaaagacata tttgaattgg      120

tttcagcaga ggccaggcca atctccaaac cgcctaattt atctggtgtc taaactggac      180

tctggggtcc cagacagatt cagcggcagt gggtcaggca ctgatttcac actgaaaatc      240

agcagggtgg aggctgagga tgttggggtt tattactgct ggcaaggtac acattttccg      300

ctcacgttcg gcggagggac caagcttgag atcaaaggag gcggatccgg aggcggaggc      360

gaagtgcagc ttgtggagtc tggaggaggc ttggtgcaac ctggaggatc cctgagactc      420

tcttgtgccg cctctggatt cacttttagt gacgcctgga tggactgggt ccgtcaggcc      480

ccaggcaagg ggcttgagtg ggttgctgaa attagaaaca aagctaaaaa tcatgcaaca      540

tactatgctg agtctgtgat agggaggttc accatctcaa gagatgacgc caaaaacagt      600

ctgtacctgc aaatgaacag cttaagagct gaagacactg ccgtgtatta ctgtggggct      660

ctgggccttg actactgggg ccaaggcacc ctggtgaccg tctccagcgc ctccaccaag      720

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc      780

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc      840

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc      900

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac      960

gtgaatcaca agcccagcaa caccaaggtg gacaagagag ttgagcccaa atcttgtgac     1020

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc     1080

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc     1140

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc     1200

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt     1260

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc     1320

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg     1380

cagccccgag aaccacaggt gtacaccctg cccccatccc gggatgagct gaccaagaac     1440

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg     1500

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac     1560

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac     1620

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc     1680

tccctgtctc cgggtaaa                                                   1698


<210>  278
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  278

Phe Asn Arg Gly Glu Cys Gly Gly Gly Ser 
1               5                   10  


<210>  279
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  279

Phe Asn Arg Gly Glu Cys Leu Gln Val Tyr Tyr Arg Met 
1               5                   10              


<210>  280
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  280

Leu Glu Gly Glu Glu Gly 
1               5       


<210>  281
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  281

Leu Glu Gly Glu Glu Gly Cys 
1               5           


<210>  282
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  282

Leu Glu Ile Lys 
1               


<210>  283
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  283

Leu Gly Glu Glu Gly 
1               5   


<210>  284
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  284

Leu Gly Glu Glu Gly Cys 
1               5       


<210>  285
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  285

Leu Gly Gly Cys Gly Gly Gly Ser 
1               5               


<210>  286
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  286

Leu Gly Lys Lys Gly 
1               5   


<210>  287
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  287

Leu Gly Lys Lys Gly Cys 
1               5       


<210>  288
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  288

Leu Lys Gly Lys Lys Gly 
1               5       


<210>  289
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  289

Leu Lys Gly Lys Lys Gly Cys 
1               5           


<210>  290
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  290

Leu Gln Val Tyr Tyr Arg Met 
1               5           


<210>  291
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  291

Leu Gln Val Tyr Tyr Arg Met Cys 
1               5               


<210>  292
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  292

Thr Val Ser Ser 
1               


<210>  293
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  293

Val Glu Pro Lys Ser Cys Gly Gly Gly Ser 
1               5                   10  


<210>  294
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  294

Val Glu Pro Lys Ser Cys Tyr Leu Tyr Leu Arg Ala Arg Val 
1               5                   10                  


<210>  295
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  295

Val Gln Val His Tyr Arg Met 
1               5           


<210>  296
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  296

Val Gln Val His Tyr Arg Met Cys 
1               5               


<210>  297
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  297

Tyr Leu Tyr Leu Arg Ala Arg Val 
1               5               


<210>  298
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  298

Tyr Leu Tyr Leu Arg Ala Arg Val Cys 
1               5                   


<210>  299
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E-coil Linker

<400>  299

Glu Val Ala Ala Leu Glu Lys Glu Val Ala Ala Leu Glu Lys Glu Val 
1               5                   10                  15      


Ala Ala Leu Glu Lys Glu Val Ala Ala Leu Glu Lys 
            20                  25              


<210>  300
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  K-coil Linker

<400>  300

Lys Val Ala Ala Leu Lys Glu Lys Val Ala Ala Leu Lys Glu Lys Val 
1               5                   10                  15      


Ala Ala Leu Lys Glu Lys Val Ala Ala Leu Lys Glu 
            20                  25              


<210>  301
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  301

Gly Gly Gly Asn Ser 
1               5   


<210>  302
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  YA2B6 light chain variable region

<400>  302

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Ile Thr Cys Arg Thr Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Lys Tyr Ala Ser Glu Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  303
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  YA2B6 hheavy chain variable region

<400>  303

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Val Ile Asp Pro Ser Asp Thr Tyr Pro Asn Tyr Asn Lys Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asn Gly Asp Ser Asp Tyr Tyr Ser Gly Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120     


<210>  304
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Albumin Binding Domain

<400>  304

Leu Ala Glu Ala Lys Val Leu Ala Asn Arg Glu Leu Asp Lys Tyr Gly 
1               5                   10                  15      


Val Ser Asp Tyr Tyr Lys Asn Leu Ile Asn Asn Ala Lys Thr Val Glu 
            20                  25                  30          


Gly Val Lys Ala Leu Ile Asp Glu Ile Leu Ala Ala Leu Pro 
        35                  40                  45      


<210>  305
<211>  279
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  h3G8VL1-G3SG4-h2B6VH4-Kcoil-GGGNS

<400>  305

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Phe Asp 
            20                  25                  30          


Gly Asp Ser Phe Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Thr Thr Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala 
        115                 120                 125             


Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser 
    130                 135                 140                 


Gly Tyr Thr Phe Thr Asn Tyr Trp Ile His Trp Val Arg Gln Ala Pro 
145                 150                 155                 160 


Gly Gln Gly Leu Glu Trp Ile Gly Val Ile Asp Pro Ser Asp Thr Tyr 
                165                 170                 175     


Pro Asn Tyr Asn Lys Lys Phe Lys Gly Arg Val Thr Met Thr Val Val 
            180                 185                 190         


Val Ser Thr Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp 
        195                 200                 205             


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Gly Asp Ser Asp Tyr Tyr 
    210                 215                 220                 


Ser Gly Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
225                 230                 235                 240 


Gly Gly Cys Gly Gly Gly Lys Val Ala Ala Leu Lys Glu Lys Val Ala 
                245                 250                 255     


Ala Leu Lys Glu Lys Val Ala Ala Leu Lys Glu Lys Val Ala Ala Leu 
            260                 265                 270         


Lys Glu Gly Gly Gly Asn Ser 
        275                 


<210>  306
<211>  837
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  h3G8VL1-G3SG4-h2B6VH4-Kcoil-GGGNS

<400>  306
gacatcgtga tgacccaatc tccagactct ttggctgtgt ctctagggga gagggccacc       60

atcaactgca aggccagcca aagtgttgat tttgatggtg atagttttat gaactggtac      120

caacagaaac caggacagcc acccaaactc ctcatctata ctacatccaa tctagaatct      180

ggggtcccag acaggtttag tggcagtggg tctgggacag acttcaccct caccatcagc      240

agcctgcagg ctgaggatgt ggcagtttat tactgtcagc aaagtaatga agatccgtac      300

acgttcggac aggggaccaa gcttgagatc aaaggaggcg gatccggcgg cggaggccag      360

gttcagctgg tgcagtctgg agctgaggtg aagaagcctg gggcctcagt gaaggtctcc      420

tgcaaggctt ctggttacac ctttaccaac tactggatac actgggtgcg acaggcccct      480

ggacaagggc ttgagtggat tggagtgatt gatccttctg atacttatcc aaattacaat      540

aaaaagttca agggcagagt caccatgacc gtagtcgtat ccacgagcac agcctacatg      600

gagctgagga gcctgagatc tgacgacacg gccgtgtatt actgtgcgag aaacggtgat      660

tccgattatt actctggtat ggactactgg gggcaaggga ccacggtcac cgtctcctcc      720

ggaggatgtg gcggtggaaa agtggccgca ctgaaggaga aagttgctgc tttgaaagag      780

aaggtcgccg cacttaagga aaaggtcgca gccctgaaag agggcggcgg gaattct         837


<210>  307
<211>  318
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  h2B6VL5-G3SG4-h3G8VH5-Ecoil-GGGNS-ABD

<400>  307

Glu Ile Val Leu Thr Gln Ser Pro Asp Phe Gln Ser Val Thr Pro Lys 
1               5                   10                  15      


Glu Lys Val Thr Phe Thr Cys Arg Thr Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Lys Glu Val Ser Glu Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys 
        115                 120                 125             


Pro Thr Gln Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu 
    130                 135                 140                 


Ser Thr Ser Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys 
145                 150                 155                 160 


Ala Leu Glu Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr 
                165                 170                 175     


Asn Pro Ala Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys 
            180                 185                 190         


Asn Gln Val Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala 
        195                 200                 205             


Thr Tyr Tyr Cys Ala Gln Ile Asn Pro Ala Trp Phe Ala Tyr Trp Gly 
    210                 215                 220                 


Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Cys Gly Gly Gly Glu 
225                 230                 235                 240 


Val Ala Ala Leu Glu Lys Glu Val Ala Ala Leu Glu Lys Glu Val Ala 
                245                 250                 255     


Ala Leu Glu Lys Glu Val Ala Ala Leu Glu Lys Gly Gly Gly Asn Ser 
            260                 265                 270         


Leu Ala Glu Ala Lys Val Leu Ala Asn Arg Glu Leu Asp Lys Tyr Gly 
        275                 280                 285             


Val Ser Asp Tyr Tyr Lys Asn Leu Ile Asn Asn Ala Lys Thr Val Glu 
    290                 295                 300                 


Gly Val Lys Ala Leu Ile Asp Glu Ile Leu Ala Ala Leu Pro 
305                 310                 315             


<210>  308
<211>  954
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  h2B6VL5-G3SG4-h3G8VH5-Ecoil-GGGNS-ABD

<400>  308
gaaattgtgc tgactcagtc tccagacttt cagtctgtga ctccaaagga gaaagtcacc       60

ttcacctgca ggaccagtca gagcattggc acaaacatac actggtacca gcagaaacca      120

gatcagtctc caaagctcct catcaaggag gtttctgagt ctatctctgg agtcccatcg      180

aggttcagtg gcagtggatc tgggacagat ttcaccctca ccatcaatag cctggaagct      240

gaagatgctg caacgtatta ctgtcaacaa agtaatacct ggccgttcac gttcggcgga      300

gggaccaagg tggagatcaa aggaggcgga tccggcggcg gaggccaggt taccctgaga      360

gagtctggcc ctgcgctggt gaagcccaca cagaccctca cactgacttg taccttctct      420

gggttttcac tgagcacttc tggtatgggt gtaggctgga ttcgtcagcc tcccgggaag      480

gctctagagt ggctggcaca catttggtgg gatgatgaca agcgctataa tccagccctg      540

aagagccgac tgacaatctc caaggatacc tccaaaaacc aggtagtcct cacaatgacc      600

aacatggacc ctgtggatac tgccacatac tactgtgctc aaataaaccc cgcctggttt      660

gcttactggg gccaagggac tctggtcact gtgagctccg gaggatgtgg cggtggagaa      720

gtggccgcac tggagaaaga ggttgctgct ttggagaagg aggtcgctgc acttgaaaag      780

gaggtcgcag ccctggagaa aggcggcggg aattctctgg ccgaagcaaa agtgctggcc      840

aaccgcgaac tggataaata tggcgtgagc gattattata agaacctgat taacaacgca      900

aagaccgtgg aaggcgtgaa agcactgatt gatgaaattc tggccgccct gcct            954


<210>  309
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  309

Ala Pro Ser Ser Ser 
1               5   


<210>  310
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  310

Val Pro Ser Met Gly Ser Ser Ser 
1               5               


<210>  311
<211>  383
<212>  PRT
<213>  Homo sapiens

<400>  311

Met Gly Leu Gly Pro Val Phe Leu Leu Leu Ala Gly Ile Phe Pro Phe 
1               5                   10                  15      


Ala Pro Pro Gly Ala Ala Ala Glu Pro His Ser Leu Arg Tyr Asn Leu 
            20                  25                  30          


Thr Val Leu Ser Trp Asp Gly Ser Val Gln Ser Gly Phe Leu Thr Glu 
        35                  40                  45              


Val His Leu Asp Gly Gln Pro Phe Leu Arg Cys Asp Arg Gln Lys Cys 
    50                  55                  60                  


Arg Ala Lys Pro Gln Gly Gln Trp Ala Glu Asp Val Leu Gly Asn Lys 
65                  70                  75                  80  


Thr Trp Asp Arg Glu Thr Arg Asp Leu Thr Gly Asn Gly Lys Asp Leu 
                85                  90                  95      


Arg Met Thr Leu Ala His Ile Lys Asp Gln Lys Glu Gly Leu His Ser 
            100                 105                 110         


Leu Gln Glu Ile Arg Val Cys Glu Ile His Glu Asp Asn Ser Thr Arg 
        115                 120                 125             


Ser Ser Gln His Phe Tyr Tyr Asp Gly Glu Leu Phe Leu Ser Gln Asn 
    130                 135                 140                 


Leu Glu Thr Lys Glu Trp Thr Met Pro Gln Ser Ser Arg Ala Gln Thr 
145                 150                 155                 160 


Leu Ala Met Asn Val Arg Asn Phe Leu Lys Glu Asp Ala Met Lys Thr 
                165                 170                 175     


Lys Thr His Tyr His Ala Met His Ala Asp Cys Leu Gln Glu Leu Arg 
            180                 185                 190         


Arg Tyr Leu Lys Ser Gly Val Val Leu Arg Arg Thr Val Pro Pro Met 
        195                 200                 205             


Val Asn Val Thr Arg Ser Glu Ala Ser Glu Gly Asn Ile Thr Val Thr 
    210                 215                 220                 


Cys Arg Ala Ser Gly Phe Tyr Pro Trp Asn Ile Thr Leu Ser Trp Arg 
225                 230                 235                 240 


Gln Asp Gly Val Ser Leu Ser His Asp Thr Gln Gln Trp Gly Asp Val 
                245                 250                 255     


Leu Pro Asp Gly Asn Gly Thr Tyr Gln Thr Trp Val Ala Thr Arg Ile 
            260                 265                 270         


Cys Gln Gly Glu Glu Gln Arg Phe Thr Cys Tyr Met Glu His Ser Gly 
        275                 280                 285             


Asn His Ser Thr His Pro Val Pro Ser Gly Lys Val Leu Val Leu Gln 
    290                 295                 300                 


Ser His Trp Gln Thr Phe His Val Ser Ala Val Ala Ala Ala Ala Ile 
305                 310                 315                 320 


Phe Val Ile Ile Ile Phe Tyr Val Arg Cys Cys Lys Lys Lys Thr Ser 
                325                 330                 335     


Ala Ala Glu Gly Pro Glu Leu Val Ser Leu Gln Val Leu Asp Gln His 
            340                 345                 350         


Pro Val Gly Thr Ser Asp His Arg Asp Ala Thr Gln Leu Gly Phe Gln 
        355                 360                 365             


Pro Leu Met Ser Asp Leu Gly Ser Thr Gly Ser Thr Glu Gly Ala 
    370                 375                 380             


<210>  312
<211>  305
<212>  PRT
<213>  Homo sapiens

<400>  312

Pro His Ser Leu Arg Tyr Asn Leu Met Val Leu Ser Gln Asp Gly Ser 
1               5                   10                  15      


Val Gln Ser Gly Phe Leu Ala Glu Gly His Leu Asp Gly Gln Pro Phe 
            20                  25                  30          


Leu Arg Tyr Asp Arg Gln Lys Arg Arg Ala Lys Pro Gln Gly Gln Trp 
        35                  40                  45              


Ala Glu Asp Val Leu Gly Ala Lys Thr Trp Asp Thr Glu Thr Glu Asp 
    50                  55                  60                  


Leu Thr Glu Asn Gly Gln Asp Leu Arg Arg Thr Leu Thr His Ile Lys 
65                  70                  75                  80  


Asp Gln Lys Gly Gly Leu His Ser Leu Gln Glu Ile Arg Val Cys Glu 
                85                  90                  95      


Ile His Glu Asp Ser Ser Thr Arg Gly Ser Arg His Phe Tyr Tyr Asp 
            100                 105                 110         


Gly Glu Leu Phe Leu Ser Gln Asn Leu Glu Thr Gln Glu Ser Thr Val 
        115                 120                 125             


Pro Gln Ser Ser Arg Ala Gln Thr Leu Ala Met Asn Val Thr Asn Phe 
    130                 135                 140                 


Trp Lys Glu Asp Ala Met Lys Thr Lys Thr His Tyr Arg Ala Met Gln 
145                 150                 155                 160 


Ala Asp Cys Leu Gln Lys Leu Gln Leu Pro Pro Met Val Asn Val Ile 
                165                 170                 175     


Cys Ser Glu Val Ser Glu Gly Asn Ile Thr Val Thr Cys Arg Ala Ser 
            180                 185                 190         


Ser Phe Tyr Pro Arg Asn Ile Thr Leu Thr Trp Arg Gln Asp Gly Val 
        195                 200                 205             


Ser Leu Ser His Asn Thr Gln Gln Trp Gly Asp Val Leu Pro Asp Gly 
    210                 215                 220                 


Asn Gly Thr Tyr Gln Thr Trp Val Ala Thr Arg Ile Arg Gln Gly Glu 
225                 230                 235                 240 


Glu Gln Arg Phe Thr Cys Tyr Met Glu His Ser Gly Asn His Gly Thr 
                245                 250                 255     


His Pro Val Pro Ser Gly Lys Ala Leu Val Leu Gln Ser Gln Arg Thr 
            260                 265                 270         


Asp Phe Pro Tyr Val Ser Ala Ala Met Pro Cys Phe Val Ile Ile Ile 
        275                 280                 285             


Ile Leu Cys Val Pro Cys Cys Lys Lys Lys Thr Ser Ala Ala Glu Gly 
    290                 295                 300                 


Pro 
305 


<210>  313
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  313

Gly Phe Asn Arg Gly Glu Cys 
1               5           


<210>  314
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  314

Gly Val Glu Pro Lys Ser Cys 
1               5           


<210>  315
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TCRVL-HER2VH

<400>  315

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Ser Ala Thr Ser Ser Val Ser Tyr Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu 
65                  70                  75                  80  


Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr 
                85                  90                  95      


Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly Gly Gly Ser Gly Gly 
            100                 105                 110         


Gly Gly Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro 
        115                 120                 125             


Gly Ala Ser Leu Lys Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile Lys 
    130                 135                 140                 


Asp Thr Tyr Ile His Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu 
145                 150                 155                 160 


Trp Ile Gly Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Asp Pro 
                165                 170                 175     


Lys Phe Gln Asp Lys Ala Thr Ile Thr Ala Asp Thr Ser Ser Asn Thr 
            180                 185                 190         


Ala Tyr Leu Gln Val Ser Arg Leu Thr Ser Glu Asp Thr Ala Val Tyr 
        195                 200                 205             


Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp 
    210                 215                 220                 


Gly Gln Gly Ala Ser Val Thr Val Ser Ser Gly Phe Asn Arg Gly Glu 
225                 230                 235                 240 


Cys 
    


<210>  316
<211>  723
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TCRVL-HER2VH

<400>  316
gaaattgtgt tgacacagtc tccagccacc ctgtctttgt ctccagggga aagagccacc       60

ctctcctgca gtgccacctc aagtgtaagt tacatgcact ggtatcagca gaaaccaggg      120

aaagccccta agcgctggat ctatgacaca tccaaactgg cttctggggt cccatcaagg      180

ttcagcggca gtggatctgg gacagaattt actctcacaa tcagcagcct gcagcctgaa      240

gattttgcaa cttattactg tcagcagtgg agtagtaacc cgctcacgtt tggccagggg      300

accaagcttg agatcaaagg aggcggatcc ggcggcggag gccaggttca gctgcagcag      360

tctgggccag agcttgtgaa gccaggggcc tcactcaagt tgtcctgtac agcttctggc      420

ttcaacatta aagacaccta tatacactgg gtgaaacaga ggcctgaaca gggcctggaa      480

tggattggaa ggatttatcc tacgaatggt tatactagat atgacccgaa gttccaggac      540

aaggccacta taacagcaga cacatcctcc aacacagcct acctgcaggt cagccgcctg      600

acatctgagg acactgccgt ctattattgt tctagatggg gaggggacgg cttctatgct      660

atggactact ggggtcaagg agcctcggtc accgtgagct ccggattcaa caggggagag      720

tgt                                                                    723


<210>  317
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2VL-TCRVH

<400>  317

Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly His Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
        115                 120                 125             


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Lys Phe 
    130                 135                 140                 


Thr Ser Tyr Val Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
145                 150                 155                 160 


Glu Trp Ile Gly Tyr Ile Asn Pro Tyr Asn Asp Val Thr Lys Tyr Asn 
                165                 170                 175     


Glu Lys Phe Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser 
            180                 185                 190         


Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
        195                 200                 205             


His Tyr Cys Ala Arg Gly Ser Tyr Tyr Asp Tyr Asp Gly Phe Val Tyr 
    210                 215                 220                 


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Val Glu Pro Lys 
225                 230                 235                 240 


Ser Cys 
        


<210>  318
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HER2VL-TCRVH

<400>  318
gacatcgtga tgacccagtc ccacaagttc atgtccacct ctgtgggcga tagggtcagc       60

atcacctgca aggccagcca ggatgtgaat actgctgtag cctggtatca gcagaaacca      120

ggacattctc ccaaactgct gatttactcc gcatccttcc ggtacactgg agtccctgat      180

cgcttcactg gcagcagatc tgggacagat ttcactttca ccatcagcag tgtgcaggct      240

gaagacctgg cagtttatta ctgtcagcaa cattatacta cacctcccac cttcggaggg      300

ggtaccaagg tggagatcaa aggaggcgga tccggcggcg gaggccaggt tcagctggtg      360

cagtctggag ctgaggtgaa gaagcctggg gcctcagtga aggtctcctg caaggccagc      420

ggttacaagt ttaccagcta cgtgatgcac tgggtgcgac aggcccctgg acaagggctt      480

gagtggatcg gatatattaa tccttacaat gatgttacta agtacaatga gaagttcaaa      540

ggcagagtca cgattaccgc ggacaaatcc acgagcacag cctacatgga gctgagcagc      600

ctgagatccg aggacacggc cgtgcactac tgtgcgagag ggagctacta tgattacgac      660

gggtttgttt actggggcca agggactctg gtcactgtga gctccggagt tgagcccaaa      720

tcttgt                                                                 726


<210>  319
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TCRVL-HER2VH-E coil

<400>  319

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Ser Ala Thr Ser Ser Val Ser Tyr Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu 
65                  70                  75                  80  


Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr 
                85                  90                  95      


Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly Gly Gly Ser Gly Gly 
            100                 105                 110         


Gly Gly Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro 
        115                 120                 125             


Gly Ala Ser Leu Lys Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile Lys 
    130                 135                 140                 


Asp Thr Tyr Ile His Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu 
145                 150                 155                 160 


Trp Ile Gly Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Asp Pro 
                165                 170                 175     


Lys Phe Gln Asp Lys Ala Thr Ile Thr Ala Asp Thr Ser Ser Asn Thr 
            180                 185                 190         


Ala Tyr Leu Gln Val Ser Arg Leu Thr Ser Glu Asp Thr Ala Val Tyr 
        195                 200                 205             


Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp 
    210                 215                 220                 


Gly Gln Gly Ala Ser Val Thr Val Ser Ser Gly Gly Cys Gly Gly Gly 
225                 230                 235                 240 


Glu Val Ala Ala Leu Glu Lys Glu Val Ala Ala Leu Glu Lys Glu Val 
                245                 250                 255     


Ala Ala Leu Glu Lys Glu Val Ala Ala Leu Glu Lys Gly Gly Gly Asn 
            260                 265                 270         


Ser 
    


<210>  320
<211>  819
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TCRVL-HER2VH-E coil

<400>  320

Gly Ala Ala Ala Thr Thr Gly Thr Gly Thr Thr Gly Ala Cys Ala Cys 
1               5                   10                  15      


Ala Gly Thr Cys Thr Cys Cys Ala Gly Cys Cys Ala Cys Cys Cys Thr 
            20                  25                  30          


Gly Thr Cys Thr Thr Thr Gly Thr Cys Thr Cys Cys Ala Gly Gly Gly 
        35                  40                  45              


Gly Ala Ala Ala Gly Ala Gly Cys Cys Ala Cys Cys Cys Thr Cys Thr 
    50                  55                  60                  


Cys Cys Thr Gly Cys Ala Gly Thr Gly Cys Cys Ala Cys Cys Thr Cys 
65                  70                  75                  80  


Ala Ala Gly Thr Gly Thr Ala Ala Gly Thr Thr Ala Cys Ala Thr Gly 
                85                  90                  95      


Cys Ala Cys Thr Gly Gly Thr Ala Thr Cys Ala Gly Cys Ala Gly Ala 
            100                 105                 110         


Ala Ala Cys Cys Ala Gly Gly Gly Ala Ala Ala Gly Cys Cys Cys Cys 
        115                 120                 125             


Thr Ala Ala Gly Cys Gly Cys Thr Gly Gly Ala Thr Cys Thr Ala Thr 
    130                 135                 140                 


Gly Ala Cys Ala Cys Ala Thr Cys Cys Ala Ala Ala Cys Thr Gly Gly 
145                 150                 155                 160 


Cys Thr Thr Cys Thr Gly Gly Gly Gly Thr Cys Cys Cys Ala Thr Cys 
                165                 170                 175     


Ala Ala Gly Gly Thr Thr Cys Ala Gly Cys Gly Gly Cys Ala Gly Thr 
            180                 185                 190         


Gly Gly Ala Thr Cys Thr Gly Gly Gly Ala Cys Ala Gly Ala Ala Thr 
        195                 200                 205             


Thr Thr Ala Cys Thr Cys Thr Cys Ala Cys Ala Ala Thr Cys Ala Gly 
    210                 215                 220                 


Cys Ala Gly Cys Cys Thr Gly Cys Ala Gly Cys Cys Thr Gly Ala Ala 
225                 230                 235                 240 


Gly Ala Thr Thr Thr Thr Gly Cys Ala Ala Cys Thr Thr Ala Thr Thr 
                245                 250                 255     


Ala Cys Thr Gly Thr Cys Ala Gly Cys Ala Gly Thr Gly Gly Ala Gly 
            260                 265                 270         


Thr Ala Gly Thr Ala Ala Cys Cys Cys Gly Cys Thr Cys Ala Cys Gly 
        275                 280                 285             


Thr Thr Thr Gly Gly Cys Cys Ala Gly Gly Gly Gly Ala Cys Cys Ala 
    290                 295                 300                 


Ala Gly Cys Thr Thr Gly Ala Gly Ala Thr Cys Ala Ala Ala Gly Gly 
305                 310                 315                 320 


Ala Gly Gly Cys Gly Gly Ala Thr Cys Cys Gly Gly Cys Gly Gly Cys 
                325                 330                 335     


Gly Gly Ala Gly Gly Cys Cys Ala Gly Gly Thr Thr Cys Ala Gly Cys 
            340                 345                 350         


Thr Gly Cys Ala Gly Cys Ala Gly Thr Cys Thr Gly Gly Gly Cys Cys 
        355                 360                 365             


Ala Gly Ala Gly Cys Thr Thr Gly Thr Gly Ala Ala Gly Cys Cys Ala 
    370                 375                 380                 


Gly Gly Gly Gly Cys Cys Thr Cys Ala Cys Thr Cys Ala Ala Gly Thr 
385                 390                 395                 400 


Thr Gly Thr Cys Cys Thr Gly Thr Ala Cys Ala Gly Cys Thr Thr Cys 
                405                 410                 415     


Thr Gly Gly Cys Thr Thr Cys Ala Ala Cys Ala Thr Thr Ala Ala Ala 
            420                 425                 430         


Gly Ala Cys Ala Cys Cys Thr Ala Thr Ala Thr Ala Cys Ala Cys Thr 
        435                 440                 445             


Gly Gly Gly Thr Gly Ala Ala Ala Cys Ala Gly Ala Gly Gly Cys Cys 
    450                 455                 460                 


Thr Gly Ala Ala Cys Ala Gly Gly Gly Cys Cys Thr Gly Gly Ala Ala 
465                 470                 475                 480 


Thr Gly Gly Ala Thr Thr Gly Gly Ala Ala Gly Gly Ala Thr Thr Thr 
                485                 490                 495     


Ala Thr Cys Cys Thr Ala Cys Gly Ala Ala Thr Gly Gly Thr Thr Ala 
            500                 505                 510         


Thr Ala Cys Thr Ala Gly Ala Thr Ala Thr Gly Ala Cys Cys Cys Gly 
        515                 520                 525             


Ala Ala Gly Thr Thr Cys Cys Ala Gly Gly Ala Cys Ala Ala Gly Gly 
    530                 535                 540                 


Cys Cys Ala Cys Thr Ala Thr Ala Ala Cys Ala Gly Cys Ala Gly Ala 
545                 550                 555                 560 


Cys Ala Cys Ala Thr Cys Cys Thr Cys Cys Ala Ala Cys Ala Cys Ala 
                565                 570                 575     


Gly Cys Cys Thr Ala Cys Cys Thr Gly Cys Ala Gly Gly Thr Cys Ala 
            580                 585                 590         


Gly Cys Cys Gly Cys Cys Thr Gly Ala Cys Ala Thr Cys Thr Gly Ala 
        595                 600                 605             


Gly Gly Ala Cys Ala Cys Thr Gly Cys Cys Gly Thr Cys Thr Ala Thr 
    610                 615                 620                 


Thr Ala Thr Thr Gly Thr Thr Cys Thr Ala Gly Ala Thr Gly Gly Gly 
625                 630                 635                 640 


Gly Ala Gly Gly Gly Gly Ala Cys Gly Gly Cys Thr Thr Cys Thr Ala 
                645                 650                 655     


Thr Gly Cys Thr Ala Thr Gly Gly Ala Cys Thr Ala Cys Thr Gly Gly 
            660                 665                 670         


Gly Gly Thr Cys Ala Ala Gly Gly Ala Gly Cys Cys Thr Cys Gly Gly 
        675                 680                 685             


Thr Cys Ala Cys Cys Gly Thr Gly Ala Gly Cys Thr Cys Cys Gly Gly 
    690                 695                 700                 


Ala Gly Gly Ala Thr Gly Thr Gly Gly Cys Gly Gly Thr Gly Gly Ala 
705                 710                 715                 720 


Gly Ala Ala Gly Thr Gly Gly Cys Cys Gly Cys Ala Cys Thr Gly Gly 
                725                 730                 735     


Ala Gly Ala Ala Ala Gly Ala Gly Gly Thr Thr Gly Cys Thr Gly Cys 
            740                 745                 750         


Thr Thr Thr Gly Gly Ala Gly Ala Ala Gly Gly Ala Gly Gly Thr Cys 
        755                 760                 765             


Gly Cys Thr Gly Cys Ala Cys Thr Thr Gly Ala Ala Ala Ala Gly Gly 
    770                 775                 780                 


Ala Gly Gly Thr Cys Gly Cys Ala Gly Cys Cys Cys Thr Gly Gly Ala 
785                 790                 795                 800 


Gly Ala Ala Ala Gly Gly Cys Gly Gly Cys Gly Gly Gly Ala Ala Thr 
                805                 810                 815     


Thr Cys Thr 
            


<210>  321
<211>  274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2VL-TCRVH-K coil

<400>  321

Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly His Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Ser Gly 
            100                 105                 110         


Gly Gly Gly Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
        115                 120                 125             


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Lys Phe 
    130                 135                 140                 


Thr Ser Tyr Val Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
145                 150                 155                 160 


Glu Trp Ile Gly Tyr Ile Asn Pro Tyr Asn Asp Val Thr Lys Tyr Asn 
                165                 170                 175     


Glu Lys Phe Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser 
            180                 185                 190         


Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
        195                 200                 205             


His Tyr Cys Ala Arg Gly Ser Tyr Tyr Asp Tyr Asp Gly Phe Val Tyr 
    210                 215                 220                 


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Cys Gly Gly 
225                 230                 235                 240 


Gly Lys Val Ala Ala Leu Lys Glu Lys Val Ala Ala Leu Lys Glu Lys 
                245                 250                 255     


Val Ala Ala Leu Lys Glu Lys Val Ala Ala Leu Lys Glu Gly Gly Gly 
            260                 265                 270         


Asn Ser 
        


<210>  322
<211>  822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HER2VL-TCRVH-K coil

<400>  322
gacatcgtga tgacccagtc ccacaagttc atgtccacct ctgtgggcga tagggtcagc       60

atcacctgca aggccagcca ggatgtgaat actgctgtag cctggtatca gcagaaacca      120

ggacattctc ccaaactgct gatttactcc gcatccttcc ggtacactgg agtccctgat      180

cgcttcactg gcagcagatc tgggacagat ttcactttca ccatcagcag tgtgcaggct      240

gaagacctgg cagtttatta ctgtcagcaa cattatacta cacctcccac cttcggaggg      300

ggtaccaagg tggagatcaa aggaggcgga tccggcggcg gaggccaggt tcagctggtg      360

cagtctggag ctgaggtgaa gaagcctggg gcctcagtga aggtctcctg caaggccagc      420

ggttacaagt ttaccagcta cgtgatgcac tgggtgcgac aggcccctgg acaagggctt      480

gagtggatcg gatatattaa tccttacaat gatgttacta agtacaatga gaagttcaaa      540

ggcagagtca cgattaccgc ggacaaatcc acgagcacag cctacatgga gctgagcagc      600

ctgagatccg aggacacggc cgtgcactac tgtgcgagag ggagctacta tgattacgac      660

gggtttgttt actggggcca agggactctg gtcactgtga gctccggagg atgtggcggt      720

ggaaaagtgg ccgcactgaa ggagaaagtt gctgctttga aagagaaggt cgccgcactt      780

aaggaaaagg tcgcagccct gaaagagggc ggcgggaatt ct                         822


<210>  323
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic ABD Construct

<400>  323

Leu Ala Glu Ala Lys Val Leu Ala Asn Arg Glu Leu Asp Lys Tyr Gly 
1               5                   10                  15      


Val Ser Asp Tyr Tyr Lys Asn Ala Ala Asn Asn Ala Lys Thr Val Glu 
            20                  25                  30          


Gly Val Lys Ala Leu Ile Ala Glu Ile Leu Ala Ala Leu Pro 
        35                  40                  45      


<210>  324
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic ABD Construct

<400>  324

Leu Ala Glu Ala Lys Val Leu Ala Asn Arg Glu Leu Asp Lys Tyr Gly 
1               5                   10                  15      


Val Ser Asp Tyr Tyr Lys Asn Leu Ile Ser Asn Ala Lys Ser Val Glu 
            20                  25                  30          


Gly Val Lys Ala Leu Ile Ala Glu Ile Leu Ala Ala Leu Pro 
        35                  40                  45      


<210>  325
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial ABD Construct

<400>  325

Tyr Tyr Lys Asn Leu Ile Asn Asn Ala Lys Thr Val Glu Gly Val Lys 
1               5                   10                  15      


Ala Leu Ile Asp Glu 
            20      


<210>  326
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic ABD Construct (ABD Y60-E80 Variant N66D/T70S/V71A )

<400>  326

Tyr Tyr Lys Asn Leu Ile Asp Asn Ala Lys Ser Ala Glu Gly Val Lys 
1               5                   10                  15      


Ala Leu Ile Asp Glu 
            20      


<210>  327
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic ABD Construct (ABD Y60-E80 Variant L64A/I65A/V71A)

<400>  327

Tyr Tyr Lys Asn Ala Ala Asn Asn Ala Lys Thr Ala Glu Gly Val Lys 
1               5                   10                  15      


Ala Leu Ile Asp Glu 
            20      


<210>  328
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic ABD Construct (L64A/I65A/V71A ("ABD (AAA)"))

<400>  328

Leu Ala Glu Ala Lys Val Leu Ala Asn Arg Glu Leu Asp Lys Tyr Gly 
1               5                   10                  15      


Val Ser Asp Tyr Tyr Lys Asn Ala Ala Asn Asn Ala Lys Thr Ala Glu 
            20                  25                  30          


Gly Val Lys Ala Leu Ile Asp Glu Ile Leu Ala Ala Leu Pro 
        35                  40                  45      


<210>  329
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic ABD Construct (N66D/T70S/V71A ("ABD (DSA)"))

<400>  329

Leu Ala Glu Ala Lys Val Leu Ala Asn Arg Glu Leu Asp Lys Tyr Gly 
1               5                   10                  15      


Val Ser Asp Tyr Tyr Lys Asn Leu Ile Asp Asn Ala Lys Ser Ala Glu 
            20                  25                  30          


Gly Val Lys Ala Leu Ile Asp Glu Ile Leu Ala Ala Leu Pro 
        35                  40                  45      


