                         SEQUENCE LISTING

<110>  Fondazione Centro San Raffaele del Monte Tabor
       FondazioneTelethon
 
<120>  New Generation of Splice-less Lentiviral Vectors for Safer Gene 
       Therapy Applications

<130>  P044270PCT

<150>  US 61/487891
<151>  2011-05-19

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  7827
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lentiviral plasmid

<400>  1
caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac       60

attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa      120

aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat      180

tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc      240

agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga      300

gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg      360

cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc      420

agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag      480

taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc      540

tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg      600

taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg      660

acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac      720

ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac      780

cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg      840

agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg      900

tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg      960

agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac     1020

tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg     1080

ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg     1140

tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc     1200

aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc     1260

tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt     1320

agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc     1380

taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact     1440

caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac     1500

agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag     1560

aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg     1620

gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg     1680

tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga     1740

gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt     1800

ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct     1860

ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg     1920

aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt     1980

aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta     2040

atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta     2100

tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt     2160

acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttggc     2220

cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat     2280

taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat     2340

tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg     2400

gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa     2460

cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact     2520

tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta     2580

aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt     2640

acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg     2700

ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg     2760

ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc     2820

cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt     2880

tagtgaaccg gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact     2940

agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc     3000

ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa     3060

aatctctagc agtggcgccc gaacagggac ctgaaagcga aagggaaacc agagctctct     3120

cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg     3180

agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc     3240

agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga     3300

aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca     3360

gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa     3420

ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc     3480

tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag     3540

gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg     3600

aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat     3660

tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag     3720

agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg     3780

cgcagcctca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca     3840

gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg     3900

gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca     3960

gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa     4020

tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg     4080

ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa     4140

ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa     4200

ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg     4260

cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg     4320

atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga     4380

aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg     4440

atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg     4500

ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat     4560

tacaaaaatt caaaatttta tcgatcacga gactagcctc gagaagcttg atatcgaatt     4620

cccacggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc     4680

tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc     4740

acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg     4800

cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga     4860

caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg     4920

gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcggg gcgcgccgag     4980

agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg     5040

ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc     5100

tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccggt cgccaccatg     5160

gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc     5220

gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc     5280

aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc     5340

gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag     5400

cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc     5460

aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg     5520

aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag     5580

ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc     5640

atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac     5700

cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac     5760

ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg     5820

ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc     5880

ggccgcgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt     5940

aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct     6000

attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt     6060

tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac     6120

gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct     6180

ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca     6240

ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt     6300

ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc     6360

ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct     6420

cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg     6480

cctggaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta     6540

gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag     6600

atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct     6660

ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag     6720

tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt     6780

cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac     6840

ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt     6900

acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta     6960

gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc     7020

cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta     7080

tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt     7140

tggaggccta ggcttttgcg tcgagacgta cccaattcgc cctatagtga gtcgtattac     7200

gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa     7260

cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc     7320

accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt     7380

agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc     7440

agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc     7500

tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg     7560

cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga     7620

tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc     7680

caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg     7740

ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt     7800

aacaaaatat taacgtttac aatttcc                                         7827


<210>  2
<211>  9771
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GLOBE plasmid

<400>  2
taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac       60

attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa      120

aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat      180

tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc      240

agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga      300

gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg      360

cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc      420

agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag      480

taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc      540

tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg      600

taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg      660

acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac      720

ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac      780

cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg      840

agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg      900

tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg      960

agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac     1020

tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg     1080

ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg     1140

tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc     1200

aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc     1260

tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt     1320

agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc     1380

taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact     1440

caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac     1500

agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag     1560

aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg     1620

gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg     1680

tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga     1740

gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt     1800

ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct     1860

ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg     1920

aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt     1980

aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta     2040

atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta     2100

tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt     2160

acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat     2220

gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc     2280

cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg     2340

tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc     2400

gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt     2460

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca     2520

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa     2580

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac     2640

agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct     2700

gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact     2760

agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt     2820

agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa     2880

catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa     2940

acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca     3000

gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata     3060

gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag     3120

accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa     3180

ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc     3240

caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt     3300

gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac     3360

ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc     3420

tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc     3480

aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg     3540

ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc     3600

tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta     3660

cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca     3720

agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg     3780

gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt     3840

ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca     3900

gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg     3960

agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac     4020

ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat     4080

agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc     4140

ggtacgtacc atgaggacag ctaaaacaat aagtaatgta aaatacagca tagcaaaact     4200

ttaacctcca aatcaagcct ctacttgaat ccttttctga gggatgaata aggcataggc     4260

atcaggggct gttgccaatg tgcattagct gtttgcagcc tcaccttctt tcatggagtt     4320

taagatatag tgtattttcc caaggtttga actagctctt catttcttta tgttttaaat     4380

gcactgacct cccacattcc ctttttagta aaatattcag aaataattta aatacatcat     4440

tgcaatgaaa ataaatgttt tttattaggc agaatccaga tgctcaaggc ccttcataat     4500

atcccccagt ttagtagttg gacttaggga acaaaggaac ctttaataga aattggacag     4560

caagaaagcg agcttagtga tacttgtggg ccagggcatt agccacacca gccaccactt     4620

tctgataggc agcctgcact ggtggggtga attctttgcc aaagtgatgg gccagcacac     4680

agaccagcac gttgcccagg agctgtggga ggaagataag aggtatgaac atgattagca     4740

aaagggccta gcttggactc agaataatcc agccttatcc caaccataaa ataaaagcag     4800

aatggtagct ggattgtagc tgctattagc aatatgaaac ctcttacatc agttacaatt     4860

tatatgcaga aataccctgt tacttctccc cttcctatga catgaactta accatagaaa     4920

agaaggggaa agaaaacatc aagggtccca tagactcacc ctgaagttct caggatccac     4980

gtgcagcttg tcacagtgca gctcactcag tgtggcaaag gtgcccttga ggttgtccag     5040

gtgagccagg ccatcactaa aggcaccgag cactttcttg ccatgagcct tcaccttagg     5100

gttgcccata acagcatcag gagtggacag atccccaaag gactcaaaga acctctgggt     5160

ccaagggtag accaccagca gcctaagggt gggaaaatag accaataggc agagagagtc     5220

agtgcctatc agaaacccaa gagtcttctc tgtctccaca tgcccagttt ctattggtct     5280

ccttaaacct gtcttgtaac cttgatacca acctgcccag ggcctcacca ccaacttcat     5340

ccacgttcac cttgccccac agggcagtaa cggcagactt ctcctcagga gtcaggtgca     5400

ccatggtgtc tgtttgaggt tgctagtgaa cacagttgtg tcagaagcaa atgtaagcaa     5460

tagatggctc tgccctgact tttatgccca gccctggctc ctgccctccc tgctcctggg     5520

agtagattgg ccaaccctag ggtgtggctc cacagggtga ggtctaagtg atgacagccg     5580

tacctgtcct tggctcttct ggcactggct taggagttgg acttcaaacc ctcagccctc     5640

cctctaagat atatctcttg gccccatacc atcagtacaa attgctacta aaaacatcct     5700

cctttgcaag tgtatttaca cggtatcgat aagcttgata tcgaattcct gcagccccct     5760

tttgccacct agctgtccag gggtgcctta aaatggcaaa caaggtttgt tttcttttcc     5820

tgttttcatg ccttcctctt ccatatcctt gtttcatatt aatacatgtg tatagatcct     5880

aaaaatctat acacatgtat taataaagcc tgattctgcc gcttctaggt atagaggcca     5940

cctgcaagat aaatatttga ttcacaataa ctaatcattc tatggcaatt gataacaaca     6000

aatatatata tatatatata tacgtatatg tgtatatata tatatatata ttcaggaaat     6060

aatatattct agaatatgtc acattctgtc tcaggcatcc attttcttta tgatgccgtt     6120

tgaggtggag ttttagtcag gtggtcagct tctccttttt tttgccatct gccctgtaag     6180

catcctgctg gggacccaga taggagtcat cactctaggc tgagaacatc tgggcacaca     6240

ccctaagcct cagcatgact catcatgact cagcattgct gtgcttgagc cagaaggttt     6300

gcttagaagg ttacacagaa ccagaaggcg ggggtggggc actgaccccg acaggggcct     6360

ggccagaact gctcatgctt ggactatggg aggtcactaa tggagacaca cagaaatgta     6420

acaggaacta aggaaaaact gaagcttatt taatcagaga tgaggatgct ggaagggata     6480

gagggagctg agcttgtaaa aagtatagta atcattcagc aaatggtttt gaagcacctg     6540

ctggatgcta aacactattt tcagtgcttg aatcataaat aagaataaaa catgtatctt     6600

attccccaca agagtccaag taaaaaataa cagttaatta taatgtgctc tgtcccccag     6660

gctggagtgc agtggcacga tctcagctca ctgcaacctc cgcctcccgg gttcaagcaa     6720

ttctcctgcc tcagccaccc taatagctgg gattacaggt gcacaccacc atgccaggct     6780

aatttttgta ctttttgtag aggttttgta ctttttgtag aggcagggta tcaccatgtt     6840

gtccaagatg gtcttgaact cctgagctcc aagcagtcca cccacctcag cctcccaaag     6900

tgctgggatt acaggtgtga gacaccatgc ccagattttc catatttaat agaggtattt     6960

atgggatggg ggaaaagaat gtttctctca ctgtggatta ttttagagag tggagaatgg     7020

tcaagatttt tttaaaaatt aagaaaacat aagttggacc ttgagaaatg aaaatttatt     7080

tttttgttgg aggataccca ttctctatct cccatcaggg caagctgtaa ggaactggct     7140

aagacacagt gagacagagt gacttagtct tagaggcccc actggtacga cggtcaccaa     7200

gctttcatta aaaaaagtct aaccagctgc attcgacttt gactgcagca gctggttaga     7260

aggttctact ggaggagggt cccagcccat tgctaaatta acatcaggct ctgagactgg     7320

cagtatatct ctaacagtgg ttgatgctat cttctggaac ttgcctgcta cattgagacc     7380

actgacccat acataggaag cccatagctc tgtcctgaac tgttaggcca ctggtccaga     7440

gagtgtgcat ctcctttgat cctcataata accctatgag atagacacaa ttattactct     7500

tactttatag atgatgatcc tgaaaacata ggagtcaagg cacttgcccc tagctggggg     7560

tataggggag cagtcccatg tagtagtaga atgaaaaatg ctgctatgct gtgcctcccc     7620

cacctttccc atgtctgccc tctactcatg gtctatctct cctggctcct gggagtcatg     7680

gactccaccc agcaccacca acctgaccta accacctatc tgagcctgcc agcctataac     7740

ccatctgggc cctgatagct ggtggccagc cctgacccca ccccaccctc cctggaacct     7800

ctgatagaca catctggcac accagctcgc aaagtcaccg tgagggtctt gtgtttgctg     7860

agtcaaaatt ccttgaaatc caagtcctta gagactcctg ctcccaaatt tacagtcata     7920

gacttcttca tggctgtctc ctttatccac agaatgattc ctttgcttca ttgccccatc     7980

catctgatcc tcctcatcag tgcagcacag ggcccatgag cagtagctgc agagtctcac     8040

ataggtctgg cactgcctct gacatgtccg accttaggca aatgcttgac tcttctgagc     8100

tcagtcttgt catggcaaaa taaagataat aatagtgttt ttttatggag ttagcgtgag     8160

gatggaaaac aatagcaaaa ttgattagac tataaaaggt ctcaacaaat agtagtagat     8220

tttatcatcc attaatcctt ccctctcctc tcttactcat cccatcacgt atgcctctta     8280

attttccctt acctataata agagttattc ctcttattat attcttctta tagtgattct     8340

ggatattaaa gtgggaatga ggggcaggcc actaacgaag aagatgtttc tcaaagaagc     8400

gggggatcca ctagttctag agcggccaaa tggcggccgt acctttaaga ccaatgactt     8460

acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa     8520

ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc     8580

agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa     8640

gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga     8700

gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc     8760

ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt     8820

ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag     8880

catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg     8940

tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc     9000

cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc     9060

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta     9120

cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt     9180

cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc     9240

gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc     9300

ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt     9360

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc     9420

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct     9480

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat     9540

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc     9600

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc     9660

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg     9720

atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat t              9771


<210>  3
<211>  7830
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lentiviral plasmid

<400>  3
caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac       60

attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa      120

aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat      180

tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc      240

agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga      300

gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg      360

cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc      420

agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag      480

taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc      540

tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg      600

taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg      660

acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac      720

ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac      780

cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg      840

agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg      900

tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg      960

agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac     1020

tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg     1080

ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg     1140

tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc     1200

aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc     1260

tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt     1320

agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc     1380

taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact     1440

caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac     1500

agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag     1560

aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg     1620

gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg     1680

tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga     1740

gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt     1800

ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct     1860

ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg     1920

aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt     1980

aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta     2040

atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta     2100

tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt     2160

acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttggc     2220

cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat     2280

taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat     2340

tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg     2400

gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa     2460

cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact     2520

tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta     2580

aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt     2640

acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg     2700

ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg     2760

ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc     2820

cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt     2880

tagtgaaccg gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact     2940

agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc     3000

ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa     3060

aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc     3120

tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg     3180

gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc     3240

gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg     3300

ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc     3360

gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta     3420

caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc     3480

ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata     3540

gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc     3600

tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa     3660

aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa     3720

aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat     3780

gggcgcagcc tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca     3840

gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt     3900

ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca     3960

acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg     4020

gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga     4080

gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca     4140

aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg     4200

gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg     4260

aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca     4320

gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc     4380

cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa     4440

cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc     4500

aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca     4560

aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgagaagc ttgatatcga     4620

attcccacgg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc     4680

ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc     4740

ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg     4800

acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg     4860

tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca     4920

atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc ggggcgcgcc     4980

gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc     5040

ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc     5100

ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatccacc ggtcgccacc     5160

atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac     5220

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac     5280

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc     5340

ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag     5400

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc     5460

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg     5520

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac     5580

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac     5640

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc     5700

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac     5760

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc     5820

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa     5880

agcggccgcg tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt     5940

cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat     6000

gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct     6060

ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct     6120

gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc     6180

gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg     6240

acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc     6300

tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac     6360

gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg     6420

cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc     6480

ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc     6540

ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac     6600

aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc     6660

tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc     6720

aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt     6780

agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat     6840

aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg     6900

gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt     6960

ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc     7020

gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat     7080

ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt     7140

ttttggaggc ctaggctttt gcgtcgagac gtacccaatt cgccctatag tgagtcgtat     7200

tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc     7260

caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc     7320

cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc     7380

tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt     7440

gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc     7500

ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta     7560

cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc     7620

tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg     7680

ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt     7740

ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat     7800

tttaacaaaa tattaacgtt tacaatttcc                                      7830
