                         SEQUENCE LISTING

<110>  DUTTA, Sheetij
 
<120>  PLASMODIUM FALCIPARUM CIRCUMSPOROZOITE VACCINE GENE OPTIMIZATION

<130>  24381WO01

<150>  US 61/394048
<151>  2010-10-18

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  834
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  recombinant Plasmodium Falciparum circumsporozoite protein 
       nucleic acid sequence

<400>  1
tacggctctt cttctaacac tcgcgtgctg aatgaactga attacgataa cgctggcacc       60

aacctgtata atgaactgga aatgaactat tacggtaagc aggaaaactg gtatagcctg      120

aaaaagaaca gccgcagcct gggtgaaaac gacgacggta acaacgagga caatgaaaaa      180

ctgcgcaagc ctaaacacaa aaagctgaaa cagccggcgg acggtaatcc ggatccaaac      240

gcaaacccga atgtggatcc gaacgccaat ccgaacgtgg acccgaacgc gaacccaaac      300

gttgatccta acgccaaccc gaacgctaac cctaacgcca acccaaacgc aaaccctaat      360

gctaacccaa acgcgaaccc gaacgcaaat ccgaacgcga accctaacgc taaccctaac      420

gcaaacccta acgcaaaccc aaacgccaac cctaacgcga acccgaatgc gaatccgaac      480

gctaatccaa atgctaaccc gaacaaaaac aaccagggca acggccaggg tcacaatatg      540

ccgaacgatc cgaatcgcaa cgtggacgaa aatgctaatg ctaacagcgc agtgaaaaac      600

aataataacg aggagccgag cgataagcac atcaaagaat atctgaacaa gatccagaat      660

agcctgtcca ccgaatggag cccgtgctct gtcacgtgcg gtaacggcat tcaagttcgt      720

atcaaaccag gtagcgccaa caagccgaaa gacgaactgg actacgcaaa cgacattgag      780

aaaaagatct gtaaaatgga aaaatgcagc tctgtcttta acgtcgttaa ctcc            834


<210>  2
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant Plasmodium Falciparum circumsporozoite polypeptide 
       sequence

<400>  2

Tyr Gly Ser Ser Ser Asn Thr Arg Val Leu Asn Glu Leu Asn Tyr Asp 
1               5                   10                  15      


Asn Ala Gly Thr Asn Leu Tyr Asn Glu Leu Glu Met Asn Tyr Tyr Gly 
            20                  25                  30          


Lys Gln Glu Asn Trp Tyr Ser Leu Lys Lys Asn Ser Arg Ser Leu Gly 
        35                  40                  45              


Glu Asn Asp Asp Gly Asn Asn Glu Asp Asn Glu Lys Leu Arg Lys Pro 
    50                  55                  60                  


Lys His Lys Lys Leu Lys Gln Pro Ala Asp Gly Asn Pro Asp Pro Asn 
65                  70                  75                  80  


Ala Asn Pro Asn Val Asp Pro Asn Ala Asn Pro Asn Val Asp Pro Asn 
                85                  90                  95      


Ala Asn Pro Asn Val Asp Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn 
            100                 105                 110         


Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn 
        115                 120                 125             


Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn 
    130                 135                 140                 


Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn 
145                 150                 155                 160 


Ala Asn Pro Asn Ala Asn Pro Asn Lys Asn Asn Gln Gly Asn Gly Gln 
                165                 170                 175     


Gly His Asn Met Pro Asn Asp Pro Asn Arg Asn Val Asp Glu Asn Ala 
            180                 185                 190         


Asn Ala Asn Ser Ala Val Lys Asn Asn Asn Asn Glu Glu Pro Ser Asp 
        195                 200                 205             


Lys His Ile Lys Glu Tyr Leu Asn Lys Ile Gln Asn Ser Leu Ser Thr 
    210                 215                 220                 


Glu Trp Ser Pro Cys Ser Val Thr Cys Gly Asn Gly Ile Gln Val Arg 
225                 230                 235                 240 


Ile Lys Pro Gly Ser Ala Asn Lys Pro Lys Asp Glu Leu Asp Tyr Ala 
                245                 250                 255     


Asn Asp Ile Glu Lys Lys Ile Cys Lys Met Glu Lys Cys Ser Ser Val 
            260                 265                 270         


Phe Asn Val Val Asn Ser 
        275             


<210>  3
<211>  33
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<223>  E. coli derived N terminal 6X-His tag nucleotide sequence derived
       from E. coli vector

<400>  3
atggcacacc atcatcatca tcatcccggg atg                                    33


<210>  4
<211>  36
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<223>  E. Coli derived C-terminal 6XHIS tag nucleotide sequence derived 
       from E. coli vector

<400>  4
ggcggccgcc tcgagcacca ccaccaccac cactga                                 36


<210>  5
<211>  902
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rCS/D; recombinant Plasmodium Falciparum circumsporozoite protein
       nucleic acid sequence with 19 NANP repeats and 3 NVDP repeats, 
       and including N and C terminal 6X HIS tags

<400>  5
atggcacacc atcatcatca tcatcccggg atgtacggct cttcttctaa cactcgcgtg       60

ctgaatgaac tgaattacga taacgctggc accaacctgt ataatgaact ggaaatgaac      120

tattacggta agcaggaaaa ctggtatagc ctgaaaaaga acagccgcag cctgggtgaa      180

aacgacgacg gtaacaacga ggacaatgaa aaactgcgca agcctaaaca caaaaagctg      240

aaacagccgg cggacggtaa tccggatcca aacgcaaacc cgaatgtgga tccgaacgca      300

atccgaacgt ggacccgaac gcgaacccaa acgttgatcc taacgccaac ccgaacgcta      360

accctaacgc caacccaaac gcaaacccta atgctaaccc aaacgcgaac ccgaacgcaa      420

atccgaacgc gaaccctaac gctaacccta acgcaaaccc taacgcaaac ccaaacgcca      480

accctaacgc gaacccgaat gcgaatccga acgctaatcc aaatgctaac ccgaacaaaa      540

acaaccaggg caacggccag ggtcacaata tgccgaacga tccgaatcgc aacgtggacg      600

aaaatgctaa tgctaacagc gcagtgaaaa acaataataa cgaggagccg agcgataagc      660

acatcaaaga atatctgaac aagatccaga atagcctgtc caccgaatgg agcccgtgct      720

ctgtcacgtg cggtaacggc attcaagttc gtatcaaacc aggtagcgcc aacaagccga      780

aagacgaact ggactacgca aacgacattg agaaaaagat ctgtaaaatg gaaaaatgca      840

gctctgtctt taacgtcgtt aactccggcg gccgcctcga gcaccaccac caccaccact      900

ga                                                                     902


<210>  6
<211>  11
<212>  PRT
<213>  Escherichia coli


<220>
<221>  MISC_FEATURE
<223>  N-terminal 6X HIS tag polypeptide sequence derived from E. coli 
       vector

<400>  6

Met Ala His His His His His His Pro Gly Met 
1               5                   10      


<210>  7
<211>  11
<212>  PRT
<213>  Escherichia coli


<220>
<221>  MISC_FEATURE
<223>  C-terminal 6X HIS tag polypeptide sequence derived from E. coli 
       vector

<400>  7

Gly Gly Arg Leu Glu His His His His His His 
1               5                   10      


<210>  8
<211>  300
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rCS/D; recombinant Plasmodium Falciparum circumsporozoite 
       polypeptide sequence including an N- and C-terminal E. Coli 
       derived 6X HIS tags and Tyr26 start amino acid of CSP

<400>  8

Met Ala His His His His His His Pro Gly Met Tyr Gly Ser Ser Ser 
1               5                   10                  15      


Asn Thr Arg Val Leu Asn Glu Leu Asn Tyr Asp Asn Ala Gly Thr Asn 
            20                  25                  30          


Leu Tyr Asn Glu Leu Glu Met Asn Tyr Tyr Gly Lys Gln Glu Asn Trp 
        35                  40                  45              


Tyr Ser Leu Lys Lys Asn Ser Arg Ser Leu Gly Glu Asn Asp Asp Gly 
    50                  55                  60                  


Asn Asn Glu Asp Asn Glu Lys Leu Arg Lys Pro Lys His Lys Lys Leu 
65                  70                  75                  80  


Lys Gln Pro Ala Asp Gly Asn Pro Asp Pro Asn Ala Asn Pro Asn Val 
                85                  90                  95      


Asp Pro Asn Ala Asn Pro Asn Val Asp Pro Asn Ala Asn Pro Asn Val 
            100                 105                 110         


Asp Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
        115                 120                 125             


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
    130                 135                 140                 


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
145                 150                 155                 160 


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
                165                 170                 175     


Asn Pro Asn Lys Asn Asn Gln Gly Asn Gly Gln Gly His Asn Met Pro 
            180                 185                 190         


Asn Asp Pro Asn Arg Asn Val Asp Glu Asn Ala Asn Ala Asn Ser Ala 
        195                 200                 205             


Val Lys Asn Asn Asn Asn Glu Glu Pro Ser Asp Lys His Ile Lys Glu 
    210                 215                 220                 


Tyr Leu Asn Lys Ile Gln Asn Ser Leu Ser Thr Glu Trp Ser Pro Cys 
225                 230                 235                 240 


Ser Val Thr Cys Gly Asn Gly Ile Gln Val Arg Ile Lys Pro Gly Ser 
                245                 250                 255     


Ala Asn Lys Pro Lys Asp Glu Leu Asp Tyr Ala Asn Asp Ile Glu Lys 
            260                 265                 270         


Lys Ile Cys Lys Met Glu Lys Cys Ser Ser Val Phe Asn Val Val Asn 
        275                 280                 285             


Ser Gly Gly Arg Leu Glu His His His His His His 
    290                 295                 300 


<210>  9
<211>  281
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rCS/A- recombinant Plasmodium Falciparum circumsporozoite 
       polypeptide sequence with 18 NANP repeats and Gln21 starting 
       amino acid and an N-terminal 6X HIS tag

<400>  9

Met Ala His His His His His His Pro Gly Gly Ser Gln Glu Tyr Gln 
1               5                   10                  15      


Cys Tyr Gly Ser Ser Ser Asn Thr Arg Val Leu Asn Glu Leu Asn Tyr 
            20                  25                  30          


Asp Asn Ala Gly Thr Asn Leu Tyr Asn Glu Leu Glu Met Asn Tyr Tyr 
        35                  40                  45              


Gly Lys Gln Glu Asn Trp Tyr Ser Leu Lys Lys Asn Ser Arg Ser Leu 
    50                  55                  60                  


Gly Glu Asn Asp Asp Gly Asn Asn Glu Asp Asn Glu Lys Leu Arg Lys 
65                  70                  75                  80  


Pro Lys His Lys Lys Leu Lys Gln Pro Ala Asp Gly Asn Pro Asn Ala 
                85                  90                  95      


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
            100                 105                 110         


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
        115                 120                 125             


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
    130                 135                 140                 


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
145                 150                 155                 160 


Asn Pro Asn Ala Asn Pro Asn Lys Asn Asn Gln Gly Asn Gly Gln Gly 
                165                 170                 175     


His Asn Met Pro Asn Asp Pro Asn Arg Asn Val Asp Glu Asn Ala Asn 
            180                 185                 190         


Ala Asn Ser Ala Val Lys Asn Asn Asn Asn Glu Glu Pro Ser Asp Lys 
        195                 200                 205             


His Ile Lys Glu Tyr Leu Asn Lys Ile Gln Asn Ser Leu Ser Thr Glu 
    210                 215                 220                 


Trp Ser Pro Cys Ser Val Thr Cys Gly Asn Gly Ile Gln Val Arg Ile 
225                 230                 235                 240 


Lys Pro Gly Ser Ala Asn Lys Pro Lys Asp Glu Leu Asp Tyr Ala Asn 
                245                 250                 255     


Asp Ile Glu Lys Lys Ile Cys Lys Met Glu Lys Cys Ser Ser Val Phe 
            260                 265                 270         


Asn Val Val Asn Ser Ser Ile Gly Leu 
        275                 280     


<210>  10
<211>  276
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rCS/B- recombinant Plasmodium Falciparum circumsporozoite 
       polypeptide sequence with 18 NANP repeats and Tyr26 start amino 
       acid and an N-terminal 6X HIS tag

<400>  10

Met Ala His His His His His His Pro Gly Gly Ser Tyr Gly Ser Ser 
1               5                   10                  15      


Ser Asn Thr Arg Val Leu Asn Glu Leu Asn Tyr Asp Asn Ala Gly Thr 
            20                  25                  30          


Asn Leu Tyr Asn Glu Leu Glu Met Asn Tyr Tyr Gly Lys Gln Glu Asn 
        35                  40                  45              


Trp Tyr Ser Leu Lys Lys Asn Ser Arg Ser Leu Gly Glu Asn Asp Asp 
    50                  55                  60                  


Gly Asn Asn Glu Asp Asn Glu Lys Leu Arg Lys Pro Lys His Lys Lys 
65                  70                  75                  80  


Leu Lys Gln Pro Ala Asp Gly Asn Pro Asn Ala Asn Pro Asn Ala Asn 
                85                  90                  95      


Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
            100                 105                 110         


Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
        115                 120                 125             


Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
    130                 135                 140                 


Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn 
145                 150                 155                 160 


Pro Asn Lys Asn Asn Gln Gly Asn Gly Gln Gly His Asn Met Pro Asn 
                165                 170                 175     


Asp Pro Asn Arg Asn Val Asp Glu Asn Ala Asn Ala Asn Ser Ala Val 
            180                 185                 190         


Lys Asn Asn Asn Asn Glu Glu Pro Ser Asp Lys His Ile Lys Glu Tyr 
        195                 200                 205             


Leu Asn Lys Ile Gln Asn Ser Leu Ser Thr Glu Trp Ser Pro Cys Ser 
    210                 215                 220                 


Val Thr Cys Gly Asn Gly Ile Gln Val Arg Ile Lys Pro Gly Ser Ala 
225                 230                 235                 240 


Asn Lys Pro Lys Asp Glu Leu Asp Tyr Ala Asn Asp Ile Glu Lys Lys 
                245                 250                 255     


Ile Cys Lys Met Glu Lys Cys Ser Ser Val Phe Asn Val Val Asn Ser 
            260                 265                 270         


Ser Ile Gly Leu 
        275     


<210>  11
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rCS/C- recombinant Plasmodium Falciparum circumsporozoite 
       polypeptide with 5 NANP repeats and 2 NVDP repeats and Tyr26 
       start amino acid and a N-terminal 6X HIS tag sequence

<400>  11

Met Ala His His His His His His Pro Gly Tyr Gly Ser Ser Ser Asn 
1               5                   10                  15      


Thr Arg Val Leu Asn Glu Leu Asn Tyr Asp Asn Ala Gly Thr Asn Leu 
            20                  25                  30          


Tyr Asn Glu Leu Glu Met Asn Tyr Tyr Gly Lys Gln Glu Asn Trp Tyr 
        35                  40                  45              


Ser Leu Lys Lys Asn Ser Arg Ser Leu Gly Glu Asn Asp Asp Gly Asn 
    50                  55                  60                  


Asn Glu Asp Asn Glu Lys Leu Arg Lys Pro Lys His Lys Lys Leu Lys 
65                  70                  75                  80  


Gln Pro Ala Asp Gly Asn Pro Asp Pro Asn Ala Asn Pro Asn Val Asp 
                85                  90                  95      


Pro Asn Ala Asn Pro Asn Val Asp Pro Asn Ala Asn Pro Asn Ala Asn 
            100                 105                 110         


Pro Asn Ala Asn Pro Asn Lys Asn Asn Gln Gly Asn Gly Gln Gly His 
        115                 120                 125             


Asn Met Pro Asn Asp Pro Asn Arg Asn Val Asp Glu Asn Ala Asn Ala 
    130                 135                 140                 


Asn Ser Ala Gly Lys Asn Asn Asn Asn Glu Glu Pro Ser Asp Lys His 
145                 150                 155                 160 


Ile Lys Glu Tyr Leu Asn Lys Ile Gln Asn Ser Leu Ser Thr Glu Trp 
                165                 170                 175     


Ser Pro Cys Ser Val Thr Cys Gly Asn Gly Ile Gln Val Arg Ile Lys 
            180                 185                 190         


Pro Gly Ser Ala Asn Lys Pro Lys Asp Glu Leu Asp Tyr Ala Asn Asp 
        195                 200                 205             


Ile Glu Lys Lys Ile Cys Lys Met Glu Lys Cys Ser Ser Val Phe Asn 
    210                 215                 220                 


Val Val Asn Ser 
225             


<210>  12
<211>  369
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rCS/E- recombinant Plasmodium Falciparum circumsporozoite 
       polypeptide with 38 NANP repeats and 4 NVDP repeats and Tyr26 
       start amino acid and a N-terminal 6X HIS tag sequence

<400>  12

Met Ala His His His His His His Pro Gly Met Tyr Gly Ser Ser Ser 
1               5                   10                  15      


Asn Thr Arg Val Leu Asn Glu Leu Asn Tyr Asp Asn Ala Gly Thr Asn 
            20                  25                  30          


Leu Tyr Asn Glu Leu Glu Met Asn Tyr Tyr Gly Lys Gln Glu Asn Trp 
        35                  40                  45              


Tyr Ser Leu Lys Lys Asn Ser Arg Ser Leu Gly Glu Asn Asp Asp Gly 
    50                  55                  60                  


Asn Asn Glu Asp Asn Glu Lys Leu Arg Lys Pro Lys His Lys Lys Leu 
65                  70                  75                  80  


Lys Gln Pro Ala Asp Gly Asn Pro Asp Pro Asn Ala Asn Pro Asn Val 
                85                  90                  95      


Asp Pro Asn Ala Asn Pro Asn Val Asp Pro Asn Ala Asn Pro Asn Val 
            100                 105                 110         


Asp Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
        115                 120                 125             


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
    130                 135                 140                 


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
145                 150                 155                 160 


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
                165                 170                 175     


Asn Pro Asn Ala Asn Pro Asn Val Asp Pro Asn Ala Asn Pro Asn Ala 
            180                 185                 190         


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
        195                 200                 205             


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
    210                 215                 220                 


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
225                 230                 235                 240 


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
                245                 250                 255     


Asn Pro Asn Lys Asn Asn Gln Gly Asn Gly Gln Gly His Asn Met Pro 
            260                 265                 270         


Asn Asp Pro Asn Arg Asn Val Asp Glu Asn Ala Asn Ala Asn Ser Ala 
        275                 280                 285             


Val Lys Asn Asn Asn Asn Glu Glu Pro Ser Asp Lys His Ile Lys Glu 
    290                 295                 300                 


Tyr Leu Asn Lys Ile Gln Asn Ser Leu Ser Thr Glu Trp Ser Pro Cys 
305                 310                 315                 320 


Ser Val Thr Cys Gly Asn Gly Ile Gln Val Arg Ile Lys Pro Gly Ser 
                325                 330                 335     


Ala Asn Lys Pro Lys Asp Glu Leu Asp Tyr Ala Asn Asp Ile Glu Lys 
            340                 345                 350         


Lys Ile Cys Lys Met Glu Lys Cys Ser Ser Val Phe Asn Val Val Asn 
        355                 360                 365             


Ser 
    


