                         SEQUENCE LISTING

<110>  DIAMOND, Don J.
       MANUEL, Edwin
 
<120>  TUMOR ASSOCIATED VACCINES AND COMPOSITIONS FOR DISRUPTING 
       TUMOR-DERIVED IMMUNOSUPPRESSION FOR USE IN COMBINATION CANCER 
       IMMUNOTHERAPY

<130>  54435.8084.WO00

<140>  PCT/US12/35512
<141>  2012-04-27

<150>  US 61/480,316
<151>  2011-04-28

<150>  US 61/615,167
<151>  2011-03-23

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shSTAT3#58

<400>  1
agttcctggc accttggatt gagagtcaa                                         29


<210>  2
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shSTAT3#59

<400>  2
actggataac ttcattagca gaatctcaa                                         29


<210>  3
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shSTAT3#60

<400>  3
catcaatcct gtggtataac atgctgacc                                         29


<210>  4
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shSTAT3#61

<400>  4
acctgaagac caagttcatc tgtgtgaca                                         29


<210>  5
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shIDO1-8

<400>  5
cctcgcaata gtagatact                                                    19


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shIDO1-9

<400>  6
cgtctctcta ttggtggaa                                                    19


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shIDO1-10

<400>  7
gcaaagaatc tcctgcaga                                                    19


<210>  8
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shIDO1-11

<400>  8
gcccatgaca tacgagaac                                                    19


<210>  9
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shIDO1-12

<400>  9
ccagtccgtg agtttgtca                                                    19


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shArg1-5

<400>  10
gcagttcctt tctggtatg                                                    19


<210>  11
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shArg1-6

<400>  11
gcctttgttg atgtccct                                                     18


<210>  12
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shArg1-7

<400>  12
ccagggactg actacctta                                                    19


<210>  13
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shArg1-8

<400>  13
gccaaagaca tcgtgtaca                                                    19


<210>  14
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shArg1-9

<400>  14
tctctacatc acagaaga                                                     18


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shiNOS-43

<400>  15
gtattgtact attgtggact a                                                 21


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shiNOS-44

<400>  16
ccagtattat ggctccttta a                                                 21


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shiNOS-45

<400>  17
gccacagcaa tataggctca t                                                 21


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shiNOS-46

<400>  18
cctatctcca ttctactact a                                                 21


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shiNOS-47

<400>  19
gctgtaacaa aggaaataga a                                                 21


<210>  20
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: STAT3 Forward

<400>  20
catgggctat aagatcatgg atgcgac                                           27


<210>  21
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: STAT3 Reverse

<400>  21
agggctcagc accttcaccg ttatttc                                           27


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GAPDH Forward

<400>  22
caaggtcatc catgacaact ttg                                               23


<210>  23
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: GAPDH Reverse

<400>  23
gtccaccacc ctgttgctgt ag                                                22


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shRNA sense sequence

<400>  24
cgtctctcta ttggtggaaa t                                                 21


<210>  25
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward IDO primer

<400>  25
ggaaccgagg ggatgacgat gttc                                              24


<210>  26
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse IDO primer

<400>  26
agactggtag ctatgtcgtg cagtgc                                            26


<210>  27
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward GAPDH primer

<400>  27
caaggtcatc catgacaact ttg                                               23


<210>  28
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse GAPDH primer

<400>  28
gtccaccacc ctgttgctgt ag                                                22


<210>  29
<211>  465
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nucleotide sequence for codon-optimized SVN (CO-SVN)

<400>  29
gatatcggcg cgccgaccct gccgccggcg tggcagccgt tcctgaaaga ccaccgtatc       60

tctaccttca aaaactggcc gttcctggaa ggctgcgcgt gcaccccgga acgtatggcg      120

gaagcgggct tcatccactg cccgaccgaa aacgaaccgg acctggcgca gtgcttcttc      180

tgcttcaaag aactggaagg ctgggaaccg gacgacgacc cgatcgaaga acacaaaaaa      240

cactcttctg gctgcgcgtt cctgtctgtt aaaaaacagt tcgaagaact gaccctgggc      300

gaattcctga aactggaccg tgaacgtgcg aaaaacaaaa tcgcgaaaga aaccaacaac      360

aaaaaaaaag aattcgaaga aaccgcgaaa aaagttcgtc gtgcgatcga acagctggcg      420

gcgatggact acccgtacga cgttccggac tacgcgtaat ctaga                      465


<210>  30
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence for codon-optimized SVN (CO-SVN)

<400>  30

Gly Ala Pro Thr Leu Pro Pro Ala Trp Gln Pro Phe Leu Lys Asp His 
1               5                   10                  15      


Arg Ile Ser Thr Phe Lys Asn Trp Pro Phe Leu Glu Gly Cys Ala Cys 
            20                  25                  30          


Thr Pro Glu Arg Met Ala Glu Ala Gly Phe Ile His Cys Pro Thr Glu 
        35                  40                  45              


Asn Glu Pro Asp Leu Ala Gln Cys Phe Phe Cys Phe Lys Glu Leu Glu 
    50                  55                  60                  


Gly Trp Glu Pro Asp Asp Asp Pro Ile Glu Glu His Lys Lys His Ser 
65                  70                  75                  80  


Ser Gly Cys Ala Phe Leu Ser Val Lys Lys Gln Phe Glu Glu Leu Thr 
                85                  90                  95      


Leu Gly Glu Phe Leu Lys Leu Asp Arg Glu Arg Ala Lys Asn Lys Ile 
            100                 105                 110         


Ala Lys Glu Thr Asn Asn Lys Lys Lys Glu Phe Glu Glu Thr Ala Lys 
        115                 120                 125             


Lys Val Arg Arg Ala Ile Glu Gln Leu Ala Ala Met Asp Tyr Pro Tyr 
    130                 135                 140                 


Asp Val Pro Asp Tyr Ala 
145                 150 


<210>  31
<211>  465
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nucleotide sequence for minimally codon-optimized SVN

<400>  31
gatatcggtg ccccgacgtt gccgcctgcc tggcagccgt ttctcaagga ccaccgcatc       60

tctacattca agaactggcc gttcttggag ggctgcgcct gcaccccgga gcggatggcc      120

gaggctggct tcatccactg cccgactgag aacgagccag acttggccca gtgtttcttc      180

tgcttcaagg agctggaagg ctgggagcca gatgacgacc cgatcgagga acataaaaag      240

cattcgtccg gttgcgcttt cctttctgtc aagaagcagt ttgaagaatt aacccttggt      300

gaatttttga aactggaccg tgaacgtgcc aagaacaaaa ttgcaaagga aaccaacaat      360

aagaagaaag aatttgagga aactgcgaag aaagtgcgcc gtgccatcga gcagctggct      420

gctatggatt acccgtacga cgttccggac tacgcgtaat ctaga                      465


<210>  32
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequenc for minimally codon-optimized SVN

<400>  32

Gly Ala Pro Thr Leu Pro Pro Ala Trp Gln Pro Phe Leu Lys Asp His 
1               5                   10                  15      


Arg Ile Ser Thr Phe Lys Asn Trp Pro Phe Leu Glu Gly Cys Ala Cys 
            20                  25                  30          


Thr Pro Glu Arg Met Ala Glu Ala Gly Phe Ile His Cys Pro Thr Glu 
        35                  40                  45              


Asn Glu Pro Asp Leu Ala Gln Cys Phe Phe Cys Phe Lys Glu Leu Glu 
    50                  55                  60                  


Gly Trp Glu Pro Asp Asp Asp Pro Ile Glu Glu His Lys Lys His Ser 
65                  70                  75                  80  


Ser Gly Cys Ala Phe Leu Ser Val Lys Lys Gln Phe Glu Glu Leu Thr 
                85                  90                  95      


Leu Gly Glu Phe Leu Lys Leu Asp Arg Glu Arg Ala Lys Asn Lys Ile 
            100                 105                 110         


Ala Lys Glu Thr Asn Asn Lys Lys Lys Glu Phe Glu Glu Thr Ala Lys 
        115                 120                 125             


Lys Val Arg Arg Ala Ile Glu Gln Leu Ala Ala Met Asp Tyr Pro Tyr 
    130                 135                 140                 


Asp Val Pro Asp Tyr Ala 
145                 150 


<210>  33
<211>  465
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nucleotide sequence for non-codon optimized eukaryotic
       SVN

<400>  33
gatatcggtg ccccgacgtt gccccctgcc tggcagccct ttctcaagga ccaccgcatc       60

tctacattca agaactggcc cttcttggag ggctgcgcct gcaccccgga gcggatggcc      120

gaggctggct tcatccactg ccccactgag aacgagccag acttggccca gtgtttcttc      180

tgcttcaagg agctggaagg ctgggagcca gatgacgacc ccatagagga acataaaaag      240

cattcgtccg gttgcgcttt cctttctgtc aagaagcagt ttgaagaatt aacccttggt      300

gaatttttga aactggacag agaaagagcc aagaacaaaa ttgcaaagga aaccaacaat      360

aagaagaaag aatttgagga aactgcgaag aaagtgcgcc gtgccatcga gcagctggct      420

gctatggatt acccatacga cgtcccagac tacgcttaat ctaga                      465


<210>  34
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Amino acid sequence for non-codon optimized eukaryotic
       SVN

<400>  34

Gly Ala Pro Thr Leu Pro Pro Ala Trp Gln Pro Phe Leu Lys Asp His 
1               5                   10                  15      


Arg Ile Ser Thr Phe Lys Asn Trp Pro Phe Leu Glu Gly Cys Ala Cys 
            20                  25                  30          


Thr Pro Glu Arg Met Ala Glu Ala Gly Phe Ile His Cys Pro Thr Glu 
        35                  40                  45              


Asn Glu Pro Asp Leu Ala Gln Cys Phe Phe Cys Phe Lys Glu Leu Glu 
    50                  55                  60                  


Gly Trp Glu Pro Asp Asp Asp Pro Ile Glu Glu His Lys Lys His Ser 
65                  70                  75                  80  


Ser Gly Cys Ala Phe Leu Ser Val Lys Lys Gln Phe Glu Glu Leu Thr 
                85                  90                  95      


Leu Gly Glu Phe Leu Lys Leu Asp Arg Glu Arg Ala Lys Asn Lys Ile 
            100                 105                 110         


Ala Lys Glu Thr Asn Asn Lys Lys Lys Glu Phe Glu Glu Thr Ala Lys 
        115                 120                 125             


Lys Val Arg Arg Ala Ile Glu Gln Leu Ala Ala Met Asp Tyr Pro Tyr 
    130                 135                 140                 


Asp Val Pro Asp Tyr Ala 
145                 150 


<210>  35
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shIDO#8

<400>  35
cctcgcaata gtagatactt actcgagtaa gtatctacta ttgcgagg                    48


<210>  36
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shIDO#9

<400>  36
cgtctctcta ttggtggaaa tctcgagatt tccaccaata gagagacg                    48


<210>  37
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shIDO#10

<400>  37
gcaaagaatc tcctgcagaa tctcgagatt ctgcaggaga ttctttgc                    48


<210>  38
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shIDO#11

<400>  38
gcccatgaca tacgagaaca tctcgagatg ttctcgtatg tcatgggc                    48


<210>  39
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shIDO#12

<400>  39
ccagtccgtg agtttgtcat tctcgagaat gacaaactca cggactgg                    48


<210>  40
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: scrambled shRNA

<400>  40
cgtgatcttc accgacaaga tctcgagatc ttgtcggtga agatcacg                    48

 

