                         SEQUENCE LISTING

<110>  Syngenta Participations AG
       Que, Qiudeng
       Nicholl, David
 
<120>  ENHANCED TRANSFORMATION OF RECALCITRANT MONOCOTS

<130>  9207-40WO

<150>  US 61/479,131
<151>  2011-04-26

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  1176
<212>  DNA
<213>  Escherichia coli

<400>  1
atgcaaaaac tcattaactc agtgcaaaac tatgcctggg gcagcaaaac ggcgttgact       60

gaactttatg gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca      120

catccgaaaa gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat      180

gtgattgaga gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa      240

ctgcctttcc tgttcaaagt attatgcgca gcacagccac tctccattca ggttcatcca      300

aacaaacaca attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat      360

gccgccgagc gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg      420

cctttccttg cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct actccagccg      480

gtcgcaggtg cacatccggc gattgctcac tttttacaac agcctgatgc cgaacgttta      540

agcgaactgt tcgccagcct gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg      600

attttaaaat cggccctcga tagccagcag ggtgaaccgt ggcaaacgat tcgtttaatt      660

tctgaatttt acccggaaga cagcggtctg ttctccccgc tattgctgaa tgtggtgaaa      720

ttgaaccctg gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc      780

gtggcgctgg aagtgatggc aaactccgat aacgtgctgc gtgcgggtct gacgcctaaa      840

tacattgata ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag      900

ttgttgaccc agccggtgaa acaaggtgca gaactggact tcccgattcc agtggatgat      960

tttgccttct cgctgcatga ccttagtgat aaagaaacca ccattagcca gcagagtgcc     1020

gccattttgt tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag     1080

cttaaaccgg gtgaatcagc gtttattgcc gccaacgaat caccggtgac tgtcaaaggc     1140

cacggccgtt tagcgcgtgt ttacaacaag ctgtaa                               1176


<210>  2
<211>  1179
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic PMI sequence with maize preferred codons

<400>  2
atgcagaagc tgatcaacag cgtgcagaac tacgcctggg gcagcaagac cgccctgacc       60

gagctgtacg gcatggagaa ccccagcagc cagcccatgg ccgagctgtg gatgggcgcc      120

caccccaaga gcagcagccg cgtgcagaac gccgccggcg acatcgtgag cctgcgcgac      180

gtgatcgaga gcgacaagag caccctgctg ggcgaggccg tggccaagcg cttcggcgag      240

ctgcccttcc tgttcaaggt gctgtgcgcc gcccagcccc tgagcatcca ggtgcacccc      300

aacaagcaca acagcgagat cggcttcgcc aaggagaacg ccgccggcat ccccatggac      360

gccgccgagc gcaactacaa ggaccccaac cacaagcccg agctggtgtt cgccctgacc      420

cccttcctgg ccatgaacgc cttccgcgag ttcagcgaga tcgtgagcct gctgcagccc      480

gtggccggcg cccaccccgc catcgcccac ttcctgcagc agcccgacgc cgagcgcctg      540

agcgagctgt tcgccagcct gctgaacatg cagggcgagg agaagagccg cgccctggcc      600

atcctgaaga gcgccctgga cagccagcag ggcgagccct ggcagaccat ccgcctgatc      660

agcgagttct accccgagga cagcggcctg ttcagccccc tgctgctgaa cgtggtgaag      720

ctgaaccccg gcgaggccat gttcctgttc gccgagaccc cccacgccta cctgcagggc      780

gtggccctgg aggtgatggc caacagcgac aacgtgctgc gcgccggcct gacccccaag      840

tacatcgaca tccccgagct ggtggccaac gtgaagttcg aggccaagcc cgccaaccag      900

ctgctgaccc agcccgtgaa gcagggcgcc gagctggact tccccatccc cgtggacgac      960

ttcgccttca gcctgcacga cctgagcgac aaggagacca ccatcagcca gcagagcgcc     1020

gccatcctgt tctgcgtgga gggcgacgcc accctgtgga agggcagcca gcagctgcag     1080

ctgaagcccg gcgagagcgc cttcatcgcc gccaacgaga gccccgtgac cgtgaagggc     1140

cacggccgcc tggcccgcgt gtacaacaag ctgtgatag                            1179


<210>  3
<211>  391
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Gln Lys Leu Ile Asn Ser Val Gln Asn Tyr Ala Trp Gly Ser Lys 
1               5                   10                  15      


Thr Ala Leu Thr Glu Leu Tyr Gly Met Glu Asn Pro Ser Ser Gln Pro 
            20                  25                  30          


Met Ala Glu Leu Trp Met Gly Ala His Pro Lys Ser Ser Ser Arg Val 
        35                  40                  45              


Gln Asn Ala Ala Gly Asp Ile Val Ser Leu Arg Asp Val Ile Glu Ser 
    50                  55                  60                  


Asp Lys Ser Thr Leu Leu Gly Glu Ala Val Ala Lys Arg Phe Gly Glu 
65                  70                  75                  80  


Leu Pro Phe Leu Phe Lys Val Leu Cys Ala Ala Gln Pro Leu Ser Ile 
                85                  90                  95      


Gln Val His Pro Asn Lys His Asn Ser Glu Ile Gly Phe Ala Lys Glu 
            100                 105                 110         


Asn Ala Ala Gly Ile Pro Met Asp Ala Ala Glu Arg Asn Tyr Lys Asp 
        115                 120                 125             


Pro Asn His Lys Pro Glu Leu Val Phe Ala Leu Thr Pro Phe Leu Ala 
    130                 135                 140                 


Met Asn Ala Phe Arg Glu Phe Ser Glu Ile Val Ser Leu Leu Gln Pro 
145                 150                 155                 160 


Val Ala Gly Ala His Pro Ala Ile Ala His Phe Leu Gln Gln Pro Asp 
                165                 170                 175     


Ala Glu Arg Leu Ser Glu Leu Phe Ala Ser Leu Leu Asn Met Gln Gly 
            180                 185                 190         


Glu Glu Lys Ser Arg Ala Leu Ala Ile Leu Lys Ser Ala Leu Asp Ser 
        195                 200                 205             


Gln Gln Gly Glu Pro Trp Gln Thr Ile Arg Leu Ile Ser Glu Phe Tyr 
    210                 215                 220                 


Pro Glu Asp Ser Gly Leu Phe Ser Pro Leu Leu Leu Asn Val Val Lys 
225                 230                 235                 240 


Leu Asn Pro Gly Glu Ala Met Phe Leu Phe Ala Glu Thr Pro His Ala 
                245                 250                 255     


Tyr Leu Gln Gly Val Ala Leu Glu Val Met Ala Asn Ser Asp Asn Val 
            260                 265                 270         


Leu Arg Ala Gly Leu Thr Pro Lys Tyr Ile Asp Ile Pro Glu Leu Val 
        275                 280                 285             


Ala Asn Val Lys Phe Glu Ala Lys Pro Ala Asn Gln Leu Leu Thr Gln 
    290                 295                 300                 


Pro Val Lys Gln Gly Ala Glu Leu Asp Phe Pro Ile Pro Val Asp Asp 
305                 310                 315                 320 


Phe Ala Phe Ser Leu His Asp Leu Ser Asp Lys Glu Thr Thr Ile Ser 
                325                 330                 335     


Gln Gln Ser Ala Ala Ile Leu Phe Cys Val Glu Gly Asp Ala Thr Leu 
            340                 345                 350         


Trp Lys Gly Ser Gln Gln Leu Gln Leu Lys Pro Gly Glu Ser Ala Phe 
        355                 360                 365             


Ile Ala Ala Asn Glu Ser Pro Val Thr Val Lys Gly His Gly Arg Leu 
    370                 375                 380                 


Ala Arg Val Tyr Asn Lys Leu 
385                 390     


<210>  4
<211>  3466
<212>  DNA
<213>  Artificial

<220>
<223>  Kozak-SynZmPMI expression cassette sequence

<400>  4
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta       60

agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta      120

tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa      180

tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga      240

gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt      300

ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg      360

gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt      420

agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata      480

taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa      540

aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga      600

cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga      660

cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg      720

acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac      780

ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc      840

gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct      900

ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca      960

cccgtcggca cctccgcttc aaggtacgcc gctcgtcctc cccccccccc cctctctacc     1020

ttctctagat cggcgttccg gtccatggtt agggcccggt agttctactt ctgttcatgt     1080

ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca cggatgcgac     1140

ctgtacgtca gacacgttct gattgctaac ttgccagtgt ttctctttgg ggaatcctgg     1200

gatggctcta gccgttccgc agacgggatc gatttcatga ttttttttgt ttcgttgcat     1260

agggtttggt ttgccctttt cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc     1320

atcttttcat gctttttttt gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc     1380

tagatcggag tagaattctg tttcaaacta cctggtggat ttattaattt tggatctgta     1440

tgtgtgtgcc atacatattc atagttacga attgaagatg atggatggaa atatcgatct     1500

aggataggta tacatgttga tgcgggtttt actgatgcat atacagagat gctttttgtt     1560

cgcttggttg tgatgatgtg gtgtggttgg gcggtcgttc attcgttcta gatcggagta     1620

gaatactgtt tcaaactacc tggtgtattt attaattttg gaactgtatg tgtgtgtcat     1680

acatcttcat agttacgagt ttaagatgga tggaaatatc gatctaggat aggtatacat     1740

gttgatgtgg gttttactga tgcatataca tgatggcata tgcagcatct attcatatgc     1800

tctaaccttg agtacctatc tattataata aacaagtatg ttttataatt attttgatct     1860

tgatatactt ggatgatggc atatgcagca gctatatgtg gattttttta gccctgcctt     1920

catacgctat ttatttgctt ggtactgttt cttttgtcga tgctcaccct gttgtttggt     1980

gttacttctg cagggatccg gcagcagcca tgcagaagct gatcaacagc gtgcagaact     2040

acgcctgggg cagcaagacc gccctgaccg agctgtacgg catggagaac cccagcagcc     2100

agcccatggc cgagctgtgg atgggcgccc accccaagag cagcagccgc gtgcagaacg     2160

ccgccggcga catcgtgagc ctgcgcgacg tgatcgagag cgacaagagc accctgctgg     2220

gcgaggccgt ggccaagcgc ttcggcgagc tgcccttcct gttcaaggtg ctgtgcgccg     2280

cccagcccct gagcatccag gtgcacccca acaagcacaa cagcgagatc ggcttcgcca     2340

aggagaacgc cgccggcatc cccatggacg ccgccgagcg caactacaag gaccccaacc     2400

acaagcccga gctggtgttc gccctgaccc ccttcctggc catgaacgcc ttccgcgagt     2460

tcagcgagat cgtgagcctg ctgcagcccg tggccggcgc ccaccccgcc atcgcccact     2520

tcctgcagca gcccgacgcc gagcgcctga gcgagctgtt cgccagcctg ctgaacatgc     2580

agggcgagga gaagagccgc gccctggcca tcctgaagag cgccctggac agccagcagg     2640

gcgagccctg gcagaccatc cgcctgatca gcgagttcta ccccgaggac agcggcctgt     2700

tcagccccct gctgctgaac gtggtgaagc tgaaccccgg cgaggccatg ttcctgttcg     2760

ccgagacccc ccacgcctac ctgcagggcg tggccctgga ggtgatggcc aacagcgaca     2820

acgtgctgcg cgccggcctg acccccaagt acatcgacat ccccgagctg gtggccaacg     2880

tgaagttcga ggccaagccc gccaaccagc tgctgaccca gcccgtgaag cagggcgccg     2940

agctggactt ccccatcccc gtggacgact tcgccttcag cctgcacgac ctgagcgaca     3000

aggagaccac catcagccag cagagcgccg ccatcctgtt ctgcgtggag ggcgacgcca     3060

ccctgtggaa gggcagccag cagctgcagc tgaagcccgg cgagagcgcc ttcatcgccg     3120

ccaacgagag ccccgtgacc gtgaagggcc acggccgcct ggcccgcgtg tacaacaagc     3180

tgtgatagga gctctagatc cccgaatttc cccgatcgtt caaacatttg gcaataaagt     3240

ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat     3300

tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt     3360

atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca     3420

aactaggata aattatcgcg cgcggtgtca tctatgttac tagatc                    3466


<210>  5
<211>  13
<212>  RNA
<213>  Artificial

<220>
<223>  Consensus Kozak sequence

<400>  5
gccgccrcca ugg                                                          13


