                         SEQUENCE LISTING

<110>  F. Hoffmann-La Roche AG
 
<120>   N-terminal acylated polypeptides, methods for their production 
       and uses thereof

<130>  27382

<150>  EP11164278.1
<151>  2011-04-29

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  62
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of the N15Q-mutant preS1(1-62; N15Q) 
       polypeptide (genotype A)

<400>  1

Met Gly Gly Trp Ser Ser Lys Pro Arg Lys Gly Met Gly Thr Gln Leu 
1               5                   10                  15      


Ser Val Pro Asn Pro Leu Gly Phe Phe Pro Asp His Gln Leu Asp Pro 
            20                  25                  30          


Ala Phe Gly Ala Asn Ser Asn Asn Pro Asp Trp Asp Phe Asn Pro Ile 
        35                  40                  45              


Lys Asp His Trp Pro Ala Ala Asn Gln Val Gly Ala Gly Ala 
    50                  55                  60          


<210>  2
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of the consensus-N17Q-mutant preS1(1-61; 
       N17Q; consensus) polypeptide

<400>  2

Met Gly Leu Gly Trp Ser Leu Ser Lys Pro Arg Gln Gly Met Gly Thr 
1               5                   10                  15      


Gln Leu Ser Val Pro Asn Pro Leu Gly Phe Phe Pro Asp His Gln Leu 
            20                  25                  30          


Asp Pro Ala Phe Gly Ala Asn Ser Asn Asn Pro Asp Trp Asp Phe Asn 
        35                  40                  45              


Pro Asn Lys Asp His Trp Pro Glu Ala Asn Gln Val Gly 
    50                  55                  60      


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker polypeptide

<400>  3

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  4
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker polypeptide

<400>  4

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  5
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker polypeptide

<400>  5

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  6
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker polypeptide

<400>  6

Gly Gly Gly Ser Gly Gly Gly Ser 
1               5               


<210>  7
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker polypeptide

<400>  7

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10          


<210>  8
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker polypeptide

<400>  8

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


<210>  9
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  9

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  10
<211>  105
<212>  PRT
<213>  Homo sapiens

<400>  10

Gln Pro Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu 
1               5                   10                  15      


Glu Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe 
            20                  25                  30          


Tyr Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val 
        35                  40                  45              


Lys Ala Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys 
    50                  55                  60                  


Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser 
65                  70                  75                  80  


His Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu 
                85                  90                  95      


Lys Thr Val Ala Pro Thr Glu Cys Ser 
            100                 105 


<210>  11
<211>  330
<212>  PRT
<213>  Homo sapiens

<400>  11

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  12
<211>  330
<212>  PRT
<213>  Homo sapiens

<400>  12

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  13
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human IgG1 (caucaisan allotype) constant region LALA variant

<400>  13

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  14
<211>  327
<212>  PRT
<213>  Homo sapiens

<400>  14

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  15
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human IgG4 constant region SPLE variant

<400>  15

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  16
<211>  231
<212>  PRT
<213>  Homo sapiens

<400>  16

Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
1               5                   10                  15      


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            20                  25                  30          


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
        35                  40                  45              


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    50                  55                  60                  


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
65                  70                  75                  80  


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                85                  90                  95      


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            100                 105                 110         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        115                 120                 125             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
    130                 135                 140                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
145                 150                 155                 160 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                165                 170                 175     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        195                 200                 205             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser Leu Ser Pro Gly Lys 
225                 230     


<210>  17
<211>  231
<212>  PRT
<213>  Homo sapiens

<400>  17

Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
1               5                   10                  15      


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            20                  25                  30          


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
        35                  40                  45              


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    50                  55                  60                  


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
65                  70                  75                  80  


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                85                  90                  95      


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            100                 105                 110         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        115                 120                 125             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
    130                 135                 140                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
145                 150                 155                 160 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                165                 170                 175     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        195                 200                 205             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser Leu Ser Pro Gly Lys 
225                 230     


<210>  18
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human IgG1 (caucasian allotype) Fc region LALA variant

<400>  18

Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
1               5                   10                  15      


Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            20                  25                  30          


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
        35                  40                  45              


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    50                  55                  60                  


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
65                  70                  75                  80  


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                85                  90                  95      


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            100                 105                 110         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        115                 120                 125             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
    130                 135                 140                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
145                 150                 155                 160 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                165                 170                 175     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        195                 200                 205             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser Leu Ser Pro Gly Lys 
225                 230     


<210>  19
<211>  234
<212>  PRT
<213>  Homo sapiens

<400>  19

Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys 
1               5                   10                  15      


Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
            20                  25                  30          


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
        35                  40                  45              


Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp 
    50                  55                  60                  


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
65                  70                  75                  80  


Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
                85                  90                  95      


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
            100                 105                 110         


Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
        115                 120                 125             


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu 
    130                 135                 140                 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
145                 150                 155                 160 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
                165                 170                 175     


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
            180                 185                 190         


Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn 
        195                 200                 205             


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
    210                 215                 220                 


Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
225                 230                 


<210>  20
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human IgG4 Fc region SPLE variant

<400>  20

Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys 
1               5                   10                  15      


Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
            20                  25                  30          


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
        35                  40                  45              


Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp 
    50                  55                  60                  


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
65                  70                  75                  80  


Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
                85                  90                  95      


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
            100                 105                 110         


Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
        115                 120                 125             


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu 
    130                 135                 140                 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
145                 150                 155                 160 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
                165                 170                 175     


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
            180                 185                 190         


Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn 
        195                 200                 205             


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
    210                 215                 220                 


Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
225                 230                 


<210>  21
<211>  165
<212>  PRT
<213>  Homo sapiens

<400>  21

Cys Asp Leu Pro Gln Thr His Ser Leu Gly Ser Arg Arg Thr Leu Met 
1               5                   10                  15      


Leu Leu Ala Gln Met Arg Lys Ile Ser Leu Phe Ser Cys Leu Lys Asp 
            20                  25                  30          


Arg His Asp Phe Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln 
        35                  40                  45              


Lys Ala Glu Thr Ile Pro Val Leu His Glu Met Ile Gln Gln Ile Phe 
    50                  55                  60                  


Asn Leu Phe Ser Thr Lys Asp Ser Ser Ala Ala Trp Asp Glu Thr Leu 
65                  70                  75                  80  


Leu Asp Lys Phe Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu 
                85                  90                  95      


Ala Cys Val Ile Gln Gly Val Gly Val Thr Glu Thr Pro Leu Met Lys 
            100                 105                 110         


Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe Gln Arg Ile Thr Leu 
        115                 120                 125             


Tyr Leu Lys Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg 
    130                 135                 140                 


Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Thr Asn Leu Gln Glu Ser 
145                 150                 155                 160 


Leu Arg Ser Lys Glu 
                165 


<210>  22
<211>  457
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of yeast NMT (N-myristoyltransferase) variant

<400>  22

Met Arg Gly Ser His His His His His His Gly Ser Leu Asn Asp Ile 
1               5                   10                  15      


Phe Glu Ala Gln Lys Ile Glu Trp His Glu Gly Ser Pro Arg Pro Pro 
            20                  25                  30          


Thr Pro Lys Ala Met Lys Asp His Lys Phe Trp Arg Thr Gln Pro Val 
        35                  40                  45              


Lys Asp Phe Asp Glu Lys Val Val Glu Glu Gly Pro Ile Asp Lys Pro 
    50                  55                  60                  


Lys Thr Pro Glu Asp Ile Ser Asp Lys Pro Leu Pro Leu Leu Ser Ser 
65                  70                  75                  80  


Phe Glu Trp Cys Ser Ile Asp Val Asp Asn Lys Lys Gln Leu Glu Asp 
                85                  90                  95      


Val Phe Val Leu Leu Asn Glu Asn Tyr Val Glu Asp Arg Asp Ala Gly 
            100                 105                 110         


Phe Arg Phe Asn Tyr Thr Lys Glu Phe Phe Asn Trp Ala Leu Lys Ser 
        115                 120                 125             


Pro Gly Trp Lys Lys Asp Trp His Ile Gly Val Arg Val Lys Glu Thr 
    130                 135                 140                 


Gln Lys Leu Val Ala Phe Ile Ser Ala Ile Pro Val Thr Leu Gly Val 
145                 150                 155                 160 


Arg Gly Lys Gln Val Pro Ser Val Glu Ile Asn Phe Leu Cys Val His 
                165                 170                 175     


Lys Gln Leu Arg Ser Lys Arg Leu Thr Pro Val Leu Ile Lys Glu Ile 
            180                 185                 190         


Thr Arg Arg Val Asn Lys Cys Asp Ile Trp His Ala Leu Tyr Thr Ala 
        195                 200                 205             


Gly Ile Val Leu Pro Ala Pro Val Ser Thr Cys Arg Tyr Thr His Arg 
    210                 215                 220                 


Pro Leu Asn Trp Lys Lys Leu Tyr Glu Val Asp Phe Thr Gly Leu Pro 
225                 230                 235                 240 


Asp Gly His Thr Glu Glu Asp Met Ile Ala Glu Asn Ala Leu Pro Ala 
                245                 250                 255     


Lys Thr Lys Thr Ala Gly Leu Arg Lys Leu Lys Lys Glu Asp Ile Asp 
            260                 265                 270         


Gln Val Phe Glu Leu Phe Lys Arg Tyr Gln Ser Arg Phe Glu Leu Ile 
        275                 280                 285             


Gln Ile Phe Thr Lys Glu Glu Phe Glu His Asn Phe Ile Gly Glu Glu 
    290                 295                 300                 


Ser Leu Pro Leu Asp Lys Gln Val Ile Phe Ser Tyr Val Val Glu Gln 
305                 310                 315                 320 


Pro Asp Gly Lys Ile Thr Asp Phe Phe Ser Phe Tyr Ser Leu Pro Phe 
                325                 330                 335     


Thr Ile Leu Asn Asn Thr Lys Tyr Lys Asp Leu Gly Ile Gly Tyr Leu 
            340                 345                 350         


Tyr Tyr Tyr Ala Thr Asp Ala Asp Phe Gln Phe Lys Asp Arg Phe Asp 
        355                 360                 365             


Pro Lys Ala Thr Lys Ala Leu Lys Thr Arg Leu Cys Glu Leu Ile Tyr 
    370                 375                 380                 


Asp Ala Cys Ile Leu Ala Lys Asn Ala Asn Met Asp Val Phe Asn Ala 
385                 390                 395                 400 


Leu Thr Ser Gln Asp Asn Thr Leu Phe Leu Asp Asp Leu Lys Phe Gly 
                405                 410                 415     


Pro Gly Asp Gly Phe Leu Asn Phe Tyr Leu Phe Asn Tyr Arg Ala Lys 
            420                 425                 430         


Pro Ile Thr Gly Gly Leu Asn Pro Asp Asn Ser Asn Asp Ile Lys Arg 
        435                 440                 445             


Arg Ser Asn Val Gly Val Val Met Leu 
    450                 455         


<210>  23
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of the mature preS1(2-62; wt)-Fc fusion

<400>  23

Gly Gly Trp Ser Ser Lys Pro Arg Lys Gly Met Gly Thr Asn Leu Ser 
1               5                   10                  15      


Val Pro Asn Pro Leu Gly Phe Phe Pro Asp His Gln Leu Asp Pro Ala 
            20                  25                  30          


Phe Gly Ala Asn Ser Asn Asn Pro Asp Trp Asp Phe Asn Pro Ile Lys 
        35                  40                  45              


Asp His Trp Pro Ala Ala Asn Gln Val Gly Ala Gly Ala Pro Lys Ser 
    50                  55                  60                  


Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
65                  70                  75                  80  


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                85                  90                  95      


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
            100                 105                 110         


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
        115                 120                 125             


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
    130                 135                 140                 


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
145                 150                 155                 160 


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
                165                 170                 175     


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
            180                 185                 190         


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
        195                 200                 205             


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
    210                 215                 220                 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
225                 230                 235                 240 


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
                245                 250                 255     


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
            260                 265                 270         


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
        275                 280                 285             


Ser Pro Gly Lys 
    290         


<210>  24
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of the mature preS1(2-62; N15Q)-Fc fusion

<400>  24

Gly Gly Trp Ser Ser Lys Pro Arg Lys Gly Met Gly Thr Gln Leu Ser 
1               5                   10                  15      


Val Pro Asn Pro Leu Gly Phe Phe Pro Asp His Gln Leu Asp Pro Ala 
            20                  25                  30          


Phe Gly Ala Asn Ser Asn Asn Pro Asp Trp Asp Phe Asn Pro Ile Lys 
        35                  40                  45              


Asp His Trp Pro Ala Ala Asn Gln Val Gly Ala Gly Ala Pro Lys Ser 
    50                  55                  60                  


Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
65                  70                  75                  80  


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                85                  90                  95      


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
            100                 105                 110         


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
        115                 120                 125             


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
    130                 135                 140                 


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
145                 150                 155                 160 


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
                165                 170                 175     


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
            180                 185                 190         


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
        195                 200                 205             


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
    210                 215                 220                 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
225                 230                 235                 240 


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
                245                 250                 255     


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
            260                 265                 270         


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
        275                 280                 285             


Ser Pro Gly Lys 
    290         


<210>  25
<211>  291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of the mature preS1(2-62; consensus, N17Q)-Fc
       fusion

<400>  25

Gly Leu Gly Trp Ser Leu Ser Lys Pro Arg Gln Gly Met Gly Thr Gln 
1               5                   10                  15      


Leu Ser Val Pro Asn Pro Leu Gly Phe Phe Pro Asp His Gln Leu Asp 
            20                  25                  30          


Pro Ala Phe Gly Ala Asn Ser Asn Asn Pro Asp Trp Asp Phe Asn Pro 
        35                  40                  45              


Asn Lys Asp His Trp Pro Glu Ala Asn Gln Val Gly Pro Lys Ser Cys 
    50                  55                  60                  


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
65                  70                  75                  80  


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
                85                  90                  95      


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
            100                 105                 110         


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
        115                 120                 125             


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
    130                 135                 140                 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
145                 150                 155                 160 


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
                165                 170                 175     


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
            180                 185                 190         


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
        195                 200                 205             


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
    210                 215                 220                 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
225                 230                 235                 240 


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
                245                 250                 255     


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
            260                 265                 270         


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
        275                 280                 285             


Pro Gly Lys 
    290     


<210>  26
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence the mature preS1(2-62; N15Q)-Fc-IFN-a2a 
       fusion protein

<400>  26

Gly Gly Trp Ser Ser Lys Pro Arg Lys Gly Met Gly Thr Gln Leu Ser 
1               5                   10                  15      


Val Pro Asn Pro Leu Gly Phe Phe Pro Asp His Gln Leu Asp Pro Ala 
            20                  25                  30          


Phe Gly Ala Asn Ser Asn Asn Pro Asp Trp Asp Phe Asn Pro Ile Lys 
        35                  40                  45              


Asp His Trp Pro Ala Ala Asn Gln Val Gly Ala Gly Ala Pro Lys Ser 
    50                  55                  60                  


Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
65                  70                  75                  80  


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                85                  90                  95      


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
            100                 105                 110         


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
        115                 120                 125             


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
    130                 135                 140                 


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
145                 150                 155                 160 


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
                165                 170                 175     


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
            180                 185                 190         


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
        195                 200                 205             


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
    210                 215                 220                 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
225                 230                 235                 240 


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
                245                 250                 255     


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
            260                 265                 270         


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
        275                 280                 285             


Ser Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Cys Asp Leu 
    290                 295                 300                 


Pro Gln Thr His Ser Leu Gly Ser Arg Arg Thr Leu Met Leu Leu Ala 
305                 310                 315                 320 


Gln Met Arg Lys Ile Ser Leu Phe Ser Cys Leu Lys Asp Arg His Asp 
                325                 330                 335     


Phe Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln Lys Ala Glu 
            340                 345                 350         


Thr Ile Pro Val Leu His Glu Met Ile Gln Gln Ile Phe Asn Leu Phe 
        355                 360                 365             


Ser Thr Lys Asp Ser Ser Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys 
    370                 375                 380                 


Phe Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu Ala Cys Val 
385                 390                 395                 400 


Ile Gln Gly Val Gly Val Thr Glu Thr Pro Leu Met Lys Glu Asp Ser 
                405                 410                 415     


Ile Leu Ala Val Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Lys 
            420                 425                 430         


Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg Ala Glu Ile 
        435                 440                 445             


Met Arg Ser Phe Ser Leu Ser Thr Asn Leu Gln Glu Ser Leu Arg Ser 
    450                 455                 460                 


Lys Glu 
465     


<210>  27
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence the knob-into-hole mature preS1(2-62; N15Q; 
       hole)-Fc  fusion protein

<400>  27

Gly Gly Trp Ser Ser Lys Pro Arg Lys Gly Met Gly Thr Gln Leu Ser 
1               5                   10                  15      


Val Pro Asn Pro Leu Gly Phe Phe Pro Asp His Gln Leu Asp Pro Ala 
            20                  25                  30          


Phe Gly Ala Asn Ser Asn Asn Pro Asp Trp Asp Phe Asn Pro Ile Lys 
        35                  40                  45              


Asp His Trp Pro Ala Ala Asn Gln Val Gly Ala Gly Ala Pro Lys Ser 
    50                  55                  60                  


Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala 
65                  70                  75                  80  


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                85                  90                  95      


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
            100                 105                 110         


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
        115                 120                 125             


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
    130                 135                 140                 


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
145                 150                 155                 160 


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
                165                 170                 175     


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
            180                 185                 190         


Val Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
        195                 200                 205             


Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
    210                 215                 220                 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
225                 230                 235                 240 


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr 
                245                 250                 255     


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
            260                 265                 270         


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
        275                 280                 285             


Ser Pro Gly Lys 
    290         


<210>  28
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence the knob-into-hole mature preS1(2-62; N15Q; 
       knob)-Fc-IFN-?2a fusion protein

<400>  28

Gly Gly Trp Ser Ser Lys Pro Arg Lys Gly Met Gly Thr Gln Leu Ser 
1               5                   10                  15      


Val Pro Asn Pro Leu Gly Phe Phe Pro Asp His Gln Leu Asp Pro Ala 
            20                  25                  30          


Phe Gly Ala Asn Ser Asn Asn Pro Asp Trp Asp Phe Asn Pro Ile Lys 
        35                  40                  45              


Asp His Trp Pro Ala Ala Asn Gln Val Gly Ala Gly Ala Pro Lys Ser 
    50                  55                  60                  


Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala 
65                  70                  75                  80  


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                85                  90                  95      


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
            100                 105                 110         


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
        115                 120                 125             


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
    130                 135                 140                 


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
145                 150                 155                 160 


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
                165                 170                 175     


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
            180                 185                 190         


Val Tyr Thr Leu Pro Pro Cys Arg Asp Glu Leu Thr Lys Asn Gln Val 
        195                 200                 205             


Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
    210                 215                 220                 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
225                 230                 235                 240 


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
                245                 250                 255     


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
            260                 265                 270         


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
        275                 280                 285             


Ser Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Cys Asp Leu 
    290                 295                 300                 


Pro Gln Thr His Ser Leu Gly Ser Arg Arg Thr Leu Met Leu Leu Ala 
305                 310                 315                 320 


Gln Met Arg Lys Ile Ser Leu Phe Ser Cys Leu Lys Asp Arg His Asp 
                325                 330                 335     


Phe Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln Lys Ala Glu 
            340                 345                 350         


Thr Ile Pro Val Leu His Glu Met Ile Gln Gln Ile Phe Asn Leu Phe 
        355                 360                 365             


Ser Thr Lys Asp Ser Ser Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys 
    370                 375                 380                 


Phe Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu Ala Cys Val 
385                 390                 395                 400 


Ile Gln Gly Val Gly Val Thr Glu Thr Pro Leu Met Lys Glu Asp Ser 
                405                 410                 415     


Ile Leu Ala Val Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Lys 
            420                 425                 430         


Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg Ala Glu Ile 
        435                 440                 445             


Met Arg Ser Phe Ser Leu Ser Thr Asn Leu Gln Glu Ser Leu Arg Ser 
    450                 455                 460                 


Lys Glu 
465     


<210>  29
<211>  62
<212>  PRT
<213>  Hepatitis B virus

<400>  29

Met Gly Gly Trp Ser Ser Lys Pro Arg Lys Gly Met Gly Thr Asn Leu 
1               5                   10                  15      


Ser Val Pro Asn Pro Leu Gly Phe Phe Pro Asp His Gln Leu Asp Pro 
            20                  25                  30          


Ala Phe Gly Ala Asn Ser Asn Asn Pro Asp Trp Asp Phe Asn Pro Ile 
        35                  40                  45              


Lys Asp His Trp Pro Ala Ala Asn Gln Val Gly Ala Gly Ala 
    50                  55                  60          


<210>  30
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of the wildtype preS1(1-62; wt) peptide

<400>  30

Met Gly Leu Gly Trp Ser Leu Ser Lys Pro Arg Gln Gly Met Gly Thr 
1               5                   10                  15      


Asn Leu Ser Val Pro Asn Pro Leu Gly Phe Phe Pro Asp His Gln Leu 
            20                  25                  30          


Asp Pro Ala Phe Gly Ala Asn Ser Asn Asn Pro Asp Trp Asp Phe Asn 
        35                  40                  45              


Pro Asn Lys Asp His Trp Pro Glu Ala Asn Gln Val Gly 
    50                  55                  60      


<210>  31
<211>  4
<212>  PRT
<213>  Escherichia coli

<400>  31

Met Arg Gly Ser 
1               


<210>  32
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  poly-histidine-tag

<400>  32

His His His His His His 
1               5       


<210>  33
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  avi-tag amino acid sequence

<400>  33

Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  


<210>  34
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgA protease cleavage site

<400>  34

Pro Arg Pro Pro Thr Pro 
1               5       


<210>  35
<211>  19
<212>  PRT
<213>  Mus musculus

<400>  35

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  36
<211>  401
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (knob)-Fc-IFN-alpha2a fusion protein

<400>  36

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Cys Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Cys Asp Leu Pro 
225                 230                 235                 240 


Gln Thr His Ser Leu Gly Ser Arg Arg Thr Leu Met Leu Leu Ala Gln 
                245                 250                 255     


Met Arg Lys Ile Ser Leu Phe Ser Cys Leu Lys Asp Arg His Asp Phe 
            260                 265                 270         


Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln Lys Ala Glu Thr 
        275                 280                 285             


Ile Pro Val Leu His Glu Met Ile Gln Gln Ile Phe Asn Leu Phe Ser 
    290                 295                 300                 


Thr Lys Asp Ser Ser Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys Phe 
305                 310                 315                 320 


Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu Ala Cys Val Ile 
                325                 330                 335     


Gln Gly Val Gly Val Thr Glu Thr Pro Leu Met Lys Glu Asp Ser Ile 
            340                 345                 350         


Leu Ala Val Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Lys Glu 
        355                 360                 365             


Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg Ala Glu Ile Met 
    370                 375                 380                 


Arg Ser Phe Ser Leu Ser Thr Asn Leu Gln Glu Ser Leu Arg Ser Lys 
385                 390                 395                 400 


Glu 
    


<210>  37
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (hole)-Fc protein

<400>  37

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Lys 
225         


