                         SEQUENCE LISTING

<110>  Aarhus Universitet
       Brinch-Pedersen, Henrik
       Madsen, Claus K
       Dionisio, Guiseppe
       Holm, Preben B
 
<120>  High expression cereal phytase gene

<130>  P1275PC00

<150>  EP11163875.5
<151>  2011-04-27

<150>  US 61/479,689
<151>  2011-04-27

<160>  59    

<170>  PatentIn version 3.5

<210>  1
<211>  15
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  mutant cereal enhancer
<222>  (1)..(15)

<220>
<221>  modified_base
<222>  (4)..(4)
<223>  V = C, G or A

<400>  1
acavgagtca tgcat                                                        15


<210>  2
<211>  19
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  mutant cereal enhancer
<222>  (1)..(19)

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  V = C, G or A

<400>  2
tagaacavga gtcatgcat                                                    19


<210>  3
<211>  15
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  wild type cereal enhancer
<222>  (1)..(15)

<400>  3
acatgagtca tgcat                                                        15


<210>  4
<211>  20
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  Wild type enhancer
<222>  (1)..(20)

<400>  4
aacatgagtc atgcatggga                                                   20


<210>  5
<211>  288
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  High Phy TaPAPhy mutant promoter
<222>  (1)..(288)
<223>  Mutant promoter and 5' untranslated region

<220>
<221>  Mutant cereal enhancer
<222>  (42)..(56)

<400>  5
ttttgttgct tgcgctttag tttcaagcta cactttgtag aacacgagtc atgcatggga       60

cgaaggcgtc caaacttggc tagtgcagct gcctgcgcgt tcacaaggca ccaaagcgca      120

ggcggcaaag tttgctcgtt tattatcttg gcggtccaag atgggcggca ggttccagac      180

gatggacgaa gacccaccga gttccacttc cggctccaac ctcctctgcc cgattcatat      240

aagtttcctg ccaaaggcat cccaattctg tcaatgccaa gcaacaac                   288


<210>  6
<211>  271
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  High Phy TaPAPhy mutant Promoter
<222>  (1)..(271)
<223>  TaPAPhy_a1 mutant promoter and 5' untranslated region

<220>
<221>  Mutant cereal enhancer
<222>  (25)..(39)

<400>  6
tagtttcaag ctacactttg tagaacacga gtcatgcatg ggacgaaggc gtccaaactt       60

ggctagtgca gctgcctgcg cgttcacaag gcaccaaagc gcaggcggca aagtttgctc      120

gtttattatc ttggcggtcc aagatgggcg gcaggttcca gacgatggac gaagacccac      180

cgagttccac ttccggctcc aacctcctct gcccgattca tataagtttc ctgccaaagg      240

catcccaatt ctgtcaatgc caagcaacaa c                                     271


<210>  7
<211>  288
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  Wild typeTaPAPhy Promoter
<222>  (1)..(288)
<223>  Wild typeTaPAPhy Promoter and 5' untranslated region

<220>
<221>  Wild type cereal enhancer
<222>  (42)..(56)

<400>  7
ttttgttgct tgcgctttag tttcaagcta cactttgtag aacatgagtc atgcatggga       60

cgaaggcgtc caaacttggc tagtgcagct gcctgcgcgt tcacaaggca ccaaagcgca      120

ggcggcaaag tttgctcgtt tattatcttg gcggtccaag atgggcggca ggttccagac      180

gatggacgaa gacccaccga gttccacttc cggctccaac ctcctctgcc cgattcatat      240

aagtttcctg ccaaaggcat tccaattctg tcaatgccaa gcaacaac                   288


<210>  8
<211>  288
<212>  DNA
<213>  Triticum tauschii


<220>
<221>  Wild type TtPAPhy promoter
<222>  (1)..(288)
<223>  Wild typeTtPAPhy Promoter and 5' untranslated region

<220>
<221>  Wild type cereal enhancer
<222>  (42)..(56)

<400>  8
ttttgttgct tgcgctttag tttcaagcta cattttgtag aacatgagtc atgcatggga       60

cgaaggcgtc caaacttggc tagtgcagct gcgtgcgcgt tcacaaggca ccaaagcgca      120

ggcggcaaag tttgctcgtt tattatcttg gcggtccaag atgggcggca ggttccagac      180

gatggacgaa gacccaccga gttccacttc cggctccaac ctcctctgcc cgattcatat      240

aagtttcctg ccaaaggcat cccaattctg tcaatgccaa gcaacaac                   288


<210>  9
<211>  271
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  Wild type TaPAPhy_a1 promoter
<222>  (1)..(271)
<223>  Wild typeTaPAPhy_a1 Promoter and 5' untranslated region

<220>
<221>  Wild type cereal enhancer
<222>  (25)..(39)

<400>  9
tagtttcaag ctacactttg tagaacatga gtcatgcatg ggacgaaggc gtccaaactt       60

ggctagtgca gctgcctgcg cgttcacaag gcaccaaagc gcaggcggca aagtttgctc      120

gtttattatc ttggcggtcc aagatgggcg gcaggttcca gacgatggac gaagacccac      180

cgagttccac ttccggctcc aacctcctct gcccgattca tataagtttc ctgccaaagg      240

cattccaatt ctgtcaatgc caagcaacaa c                                     271


<210>  10
<211>  287
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  Wild type TaPAPhy_a3 promoter
<222>  (1)..(287)
<223>  Wild type TaPAPhy_a3 promoter and 5' non-coding region

<220>
<221>  Wild type cereal enhancer
<222>  (25)..(39)

<400>  10
tagtttcaag ctacattttg tagaacatga gtcatgcatg ggacgaaggt gtccaaagtc       60

caaactcggc tagtgcagct gcctgcacgt tctgacgttc acaaggcacc aaagcgcagg      120

cggcaaactt tgctcgttta ttatctcgcc ggtccaagat gggcggcaag ttctagacgc      180

tggacgaaga cccaccgaat tccatttccg gctcccaacc tcctctgccc gattcctgta      240

agtttcctgc caaaatcatc ccaattctct caatgccaag caacacc                    287


<210>  11
<211>  240
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  Wild type TaPAPhy_a4 promoter
<222>  (1)..(240)
<223>  Wild type TaPAPhy_a4 promoter and 5' non-coding region

<220>
<221>  Wild type cereal enhancer
<222>  (25)..(39)

<400>  11
tattttcaag ctacattttg tagaacatga gtcatgcatg ggacgaaggt ggccaaagtc       60

caaacttggc aggcggcaaa gtttgctcgt ttatcatctt gccggtccaa gatgggcggc      120

aggttccagg cgatggacga agacccaccg agtcccactt ccggctccca acctcctctg      180

cccgattcat ataagtttcc tgccaaaggc atcctaattc tgtcaatacc aagcaacaac      240


<210>  12
<211>  278
<212>  DNA
<213>  Triticum monococcum


<220>
<221>  High Phy TmPAPhy mutant promoter
<222>  (1)..(278)
<223>  High Phy TmPAPhy mutant promoter and 5' untranslated region

<220>
<221>  mutant cereal enhancer
<222>  (25)..(39)

<400>  12
tagtttcaag ctacattttg tagaacagga gtcatgcatg gacgaaggtg tccaaagtcc       60

aaacttggct agcgcagctg cctgcacgtt cacaaggcac caaagcgcag gcggcaaagt      120

ttgctcgttt attatcttgc cggtccaaga cgggcggcag gttccagacg atggacgaag      180

acccaccgaa ttccatttcc ggctcccaac ctcctctgcc cgattcctac aagtttcctg      240

ccaaaggcat cccaattctg tcaatgccaa gcaacgcc                              278


<210>  13
<211>  262
<212>  DNA
<213>  Hordeum vulgare


<220>
<221>  Wild type HvPAPhy promoter
<222>  (1)..(262)
<223>  Wild type HvPAPhy promoter and 5' untranslated region

<220>
<221>  Wild type enhancer
<222>  (25)..(39)
<223>  Wild type cereal enhancer

<400>  13
taatttcaag ctacattttg tagaacatga gccatgcatg agacgtaggc gtccaaactt       60

tggctagcgc agctgcatgc acgtccacaa ggcaccaaag gcgcaggcgg caactttgct      120

cgtttatttt cttgcgggtc caagatgagt tccagaccat ggacgaattc cacttcgggc      180

tcccaatctc ctctgccgga ttcctataag tttcctgcca agaagcatcc caatcccctc      240

aatgccaagc aacaacatca ac                                               262


<210>  14
<211>  262
<212>  DNA
<213>  Hordeum vulgare


<220>
<221>  High Phy HvPAPhy mutant promoter
<222>  (1)..(262)
<223>  High Phy HvPAPhy mutant promoter and 5' non-coding region

<220>
<221>  Mutant cereal enhancer
<222>  (25)..(39)

<400>  14
taatttcaag ctacattttg tagaacacga gccatgcatg agacgtaggc gtccaaactt       60

tggctagcgc agctgcatgc acgtccacaa ggcaccaaag gcgcaggcgg caactttgct      120

cgtttatttt cttgcgggtc caagatgagt tccagaccat ggacgaattc cacttcgggc      180

tcccaatctc ctctgccgga ttcctataag tttcctgcca agaagcatcc caatcccctc      240

aatgccaagc aacaacatca ac                                               262


<210>  15
<211>  270
<212>  DNA
<213>  Secale cereale


<220>
<221>  HighPhy ScPAPhy_a1 promoter
<222>  (1)..(270)
<223>  HighPhy ScPAPhy_a1 promoter and 5' non-coding region

<220>
<221>  mutant cereal enhancer
<222>  (25)..(39)

<400>  15
tagtttcaag ctacattttc tagaacacga gtcatgcatg ggacgaaggt gtccaaagtc       60

caaacttggc ttttgtgcag ctgcctgcac gttcacaagg caccaaagcg caggcggcaa      120

acttaatttg ctcgttcatt atcttgctgg tccaagatgg gcggcaggtt gcacccaccg      180

agttccactt ccggctccca atctcctgtg cctgattcct ataagtttcc tgccaaaagc      240

atcccaattc tgtcaatgcc aagcaacaac                                       270


<210>  16
<211>  280
<212>  DNA
<213>  Secale cereale


<220>
<221>  Wild type ScPAPhy_a2 promoter
<222>  (1)..(280)
<223>  Wild type ScPAPhy_a2 promoter and 5' non-coding region

<220>
<221>  wild type cereal enhancer
<222>  (25)..(39)

<400>  16
agtttcaagc tacattttgt agaacatgag tcatgcatgg gacgaaggtg tccaaagtcc       60

aaacttggct tttgtgcagc tgcctgcacg ttcacaaggc accaaagcgc aggcggcaaa      120

ctttgctcgt tcattatctt gctggtccaa gatgggcggc aggttgcaga agatggacga      180

agacccaccg agttccactt ccggctccca atcgcctctg cccgattcct ataagtttcc      240

tgccaaaggc atcccaattc tgtcaatgcc aagcaacaac                            280


<210>  17
<211>  1749
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  TaPAPhy_a1 phytase cDNA
<222>  (1)..(1749)

<220>
<221>  CDS
<222>  (27)..(1670)
<223>  Coding sequence for phytase

<400>  17
caattctgtc aatgccaagc aacaac atg tgg tgg ggg tcg ctg ctg ctg ctg        53
                             Met Trp Trp Gly Ser Leu Leu Leu Leu          
                             1               5                            

ctg ctg ctc gcg gcc gcg gtg gcg gcg gct gct gag ccg gcg tcg acg        101
Leu Leu Leu Ala Ala Ala Val Ala Ala Ala Ala Glu Pro Ala Ser Thr           
10                  15                  20                  25            

ctc acg ggc ccg tca cgg ccg gtc acg gtg gcg ctg cgg gaa gac agg        149
Leu Thr Gly Pro Ser Arg Pro Val Thr Val Ala Leu Arg Glu Asp Arg           
                30                  35                  40                

ggc cac gcg gtg gac ctg ccg gac acg gac ccc cgg gtg cag cgc cgg        197
Gly His Ala Val Asp Leu Pro Asp Thr Asp Pro Arg Val Gln Arg Arg           
            45                  50                  55                    

gcc acg ggc tgg gct ccc gag cag atc gcc gtc gcg ctc tcc gcc gct        245
Ala Thr Gly Trp Ala Pro Glu Gln Ile Ala Val Ala Leu Ser Ala Ala           
        60                  65                  70                        

ccc acc tct gcc tgg gtc tcc tgg atc acc ggg gaa ttc cag atg ggc        293
Pro Thr Ser Ala Trp Val Ser Trp Ile Thr Gly Glu Phe Gln Met Gly           
    75                  80                  85                            

ggc acc gtc aag ccg ctg gac ccc ggc acg gtc ggc agc gtc gtg cgc        341
Gly Thr Val Lys Pro Leu Asp Pro Gly Thr Val Gly Ser Val Val Arg           
90                  95                  100                 105           

tac ggg ctc gcc gcc gat tct ttg gtt cgc cag gcc agc ggc gac gcg        389
Tyr Gly Leu Ala Ala Asp Ser Leu Val Arg Gln Ala Ser Gly Asp Ala           
                110                 115                 120               

ctc gtg tac agc cag ctc tac ccc ttc gag ggt ctc cag aac tac acc        437
Leu Val Tyr Ser Gln Leu Tyr Pro Phe Glu Gly Leu Gln Asn Tyr Thr           
            125                 130                 135                   

tcc ggc atc atc cac cac gtc cgc ctc caa ggg ctt gag cct gcg acg        485
Ser Gly Ile Ile His His Val Arg Leu Gln Gly Leu Glu Pro Ala Thr           
        140                 145                 150                       

aag tac tac tac cag tgc ggc gac ccg gcc ctc ccg ggg gcg atg agc        533
Lys Tyr Tyr Tyr Gln Cys Gly Asp Pro Ala Leu Pro Gly Ala Met Ser           
    155                 160                 165                           

gcc gtc cac gcg ttc cgg acg atg ccg gcg gtg ggg ccg cgg agc tac        581
Ala Val His Ala Phe Arg Thr Met Pro Ala Val Gly Pro Arg Ser Tyr           
170                 175                 180                 185           

ccg ggg agg atc gcc gtg gtg gga gac ctc ggg ctc acg tac aac acc        629
Pro Gly Arg Ile Ala Val Val Gly Asp Leu Gly Leu Thr Tyr Asn Thr           
                190                 195                 200               

acc tcc acc gtg gac cac atg gcg agc aac cgg ccg gac ctg gtc ctc        677
Thr Ser Thr Val Asp His Met Ala Ser Asn Arg Pro Asp Leu Val Leu           
            205                 210                 215                   

ctc gtc ggc gac gtg tgc tac gcc aac atg tac ctc acc aac ggc acc        725
Leu Val Gly Asp Val Cys Tyr Ala Asn Met Tyr Leu Thr Asn Gly Thr           
        220                 225                 230                       

gga gcg gac tgc tac tcg tgc gcg ttc ggc aag tcg acg ccc atc cac        773
Gly Ala Asp Cys Tyr Ser Cys Ala Phe Gly Lys Ser Thr Pro Ile His           
    235                 240                 245                           

gag acg tac cag ccg cgc tgg gac tac tgg gga agg tac atg gag gcg        821
Glu Thr Tyr Gln Pro Arg Trp Asp Tyr Trp Gly Arg Tyr Met Glu Ala           
250                 255                 260                 265           

gtg acg tcg ggg acg ccg atg atg gtg gtg gaa ggg aac cat gag ata        869
Val Thr Ser Gly Thr Pro Met Met Val Val Glu Gly Asn His Glu Ile           
                270                 275                 280               

gag gag cag atc ggg aac aag acg ttc gcg gcc tac cgc tcc cgg ttc        917
Glu Glu Gln Ile Gly Asn Lys Thr Phe Ala Ala Tyr Arg Ser Arg Phe           
            285                 290                 295                   

gcg ttc ccg tcg acg gag agc ggg tcc ttc tcc ccc ttc tac tac tcg        965
Ala Phe Pro Ser Thr Glu Ser Gly Ser Phe Ser Pro Phe Tyr Tyr Ser           
        300                 305                 310                       

ttc gac gcc ggc ggg atc cat ttc ctc atg ctc ggc gcc tac gcc gac       1013
Phe Asp Ala Gly Gly Ile His Phe Leu Met Leu Gly Ala Tyr Ala Asp           
    315                 320                 325                           

tac ggc agg tca ggg gag cag tac aga tgg ctg gag aag gac ctg gcg       1061
Tyr Gly Arg Ser Gly Glu Gln Tyr Arg Trp Leu Glu Lys Asp Leu Ala           
330                 335                 340                 345           

aag gtg gac agg tcg gtg acg ccg tgg ctg gtc gcc ggc tgg cac gcg       1109
Lys Val Asp Arg Ser Val Thr Pro Trp Leu Val Ala Gly Trp His Ala           
                350                 355                 360               

cca tgg tac acc acc tac aag gct cac tac agg gag gtg gag tgc atg       1157
Pro Trp Tyr Thr Thr Tyr Lys Ala His Tyr Arg Glu Val Glu Cys Met           
            365                 370                 375                   

aga gtg gcc atg gag gag ctg ctc tac tcc cac ggc ctc gac atc gcc       1205
Arg Val Ala Met Glu Glu Leu Leu Tyr Ser His Gly Leu Asp Ile Ala           
        380                 385                 390                       

ttc acc ggc cat gtg cac gcg tat gag cgc tcc aac cgg gtg ttc aac       1253
Phe Thr Gly His Val His Ala Tyr Glu Arg Ser Asn Arg Val Phe Asn           
    395                 400                 405                           

tac acg ctg gac ccg tgc ggc gcc gtg cac atc tcg gtg ggc gac ggc       1301
Tyr Thr Leu Asp Pro Cys Gly Ala Val His Ile Ser Val Gly Asp Gly           
410                 415                 420                 425           

ggg aac cgc gag aag atg gcc acc acc cac gcc gac gag ccg ggg cac       1349
Gly Asn Arg Glu Lys Met Ala Thr Thr His Ala Asp Glu Pro Gly His           
                430                 435                 440               

tgc ccg gac ccg cgg ccc aag ccc aac gcc ttc atc ggc ggc ttc tgc       1397
Cys Pro Asp Pro Arg Pro Lys Pro Asn Ala Phe Ile Gly Gly Phe Cys           
            445                 450                 455                   

gcc tcc aac ttc acg tcc ggc ccg gcc gcc ggc agg ttc tgc tgg gac       1445
Ala Ser Asn Phe Thr Ser Gly Pro Ala Ala Gly Arg Phe Cys Trp Asp           
        460                 465                 470                       

cgg cag ccg gac tac agc gcc tac cgg gag agc agc ttc ggc cac ggc       1493
Arg Gln Pro Asp Tyr Ser Ala Tyr Arg Glu Ser Ser Phe Gly His Gly           
    475                 480                 485                           

atc ctc gag gtg aag aac gag acg cac gct ctg tgg aga tgg cac agg       1541
Ile Leu Glu Val Lys Asn Glu Thr His Ala Leu Trp Arg Trp His Arg           
490                 495                 500                 505           

aac cag gac cac tac ggg agc gcc gga gat gag att tac att gtc cgg       1589
Asn Gln Asp His Tyr Gly Ser Ala Gly Asp Glu Ile Tyr Ile Val Arg           
                510                 515                 520               

gag ccg cac agg tgc ttg cac aag cac aac tcg agc agg ccg gca cac       1637
Glu Pro His Arg Cys Leu His Lys His Asn Ser Ser Arg Pro Ala His           
            525                 530                 535                   

ggt cga tca aac acc aca cgg gaa tcg gga ggt taaccgttgt accactggag     1690
Gly Arg Ser Asn Thr Thr Arg Glu Ser Gly Gly                               
        540                 545                                           

tagatcgcgt ggtgtaatgg caactgtata gacggttcgc ccaagcgtgg aaataaaaa      1749


<210>  18
<211>  548
<212>  PRT
<213>  Triticum aestivum

<400>  18

Met Trp Trp Gly Ser Leu Leu Leu Leu Leu Leu Leu Ala Ala Ala Val 
1               5                   10                  15      


Ala Ala Ala Ala Glu Pro Ala Ser Thr Leu Thr Gly Pro Ser Arg Pro 
            20                  25                  30          


Val Thr Val Ala Leu Arg Glu Asp Arg Gly His Ala Val Asp Leu Pro 
        35                  40                  45              


Asp Thr Asp Pro Arg Val Gln Arg Arg Ala Thr Gly Trp Ala Pro Glu 
    50                  55                  60                  


Gln Ile Ala Val Ala Leu Ser Ala Ala Pro Thr Ser Ala Trp Val Ser 
65                  70                  75                  80  


Trp Ile Thr Gly Glu Phe Gln Met Gly Gly Thr Val Lys Pro Leu Asp 
                85                  90                  95      


Pro Gly Thr Val Gly Ser Val Val Arg Tyr Gly Leu Ala Ala Asp Ser 
            100                 105                 110         


Leu Val Arg Gln Ala Ser Gly Asp Ala Leu Val Tyr Ser Gln Leu Tyr 
        115                 120                 125             


Pro Phe Glu Gly Leu Gln Asn Tyr Thr Ser Gly Ile Ile His His Val 
    130                 135                 140                 


Arg Leu Gln Gly Leu Glu Pro Ala Thr Lys Tyr Tyr Tyr Gln Cys Gly 
145                 150                 155                 160 


Asp Pro Ala Leu Pro Gly Ala Met Ser Ala Val His Ala Phe Arg Thr 
                165                 170                 175     


Met Pro Ala Val Gly Pro Arg Ser Tyr Pro Gly Arg Ile Ala Val Val 
            180                 185                 190         


Gly Asp Leu Gly Leu Thr Tyr Asn Thr Thr Ser Thr Val Asp His Met 
        195                 200                 205             


Ala Ser Asn Arg Pro Asp Leu Val Leu Leu Val Gly Asp Val Cys Tyr 
    210                 215                 220                 


Ala Asn Met Tyr Leu Thr Asn Gly Thr Gly Ala Asp Cys Tyr Ser Cys 
225                 230                 235                 240 


Ala Phe Gly Lys Ser Thr Pro Ile His Glu Thr Tyr Gln Pro Arg Trp 
                245                 250                 255     


Asp Tyr Trp Gly Arg Tyr Met Glu Ala Val Thr Ser Gly Thr Pro Met 
            260                 265                 270         


Met Val Val Glu Gly Asn His Glu Ile Glu Glu Gln Ile Gly Asn Lys 
        275                 280                 285             


Thr Phe Ala Ala Tyr Arg Ser Arg Phe Ala Phe Pro Ser Thr Glu Ser 
    290                 295                 300                 


Gly Ser Phe Ser Pro Phe Tyr Tyr Ser Phe Asp Ala Gly Gly Ile His 
305                 310                 315                 320 


Phe Leu Met Leu Gly Ala Tyr Ala Asp Tyr Gly Arg Ser Gly Glu Gln 
                325                 330                 335     


Tyr Arg Trp Leu Glu Lys Asp Leu Ala Lys Val Asp Arg Ser Val Thr 
            340                 345                 350         


Pro Trp Leu Val Ala Gly Trp His Ala Pro Trp Tyr Thr Thr Tyr Lys 
        355                 360                 365             


Ala His Tyr Arg Glu Val Glu Cys Met Arg Val Ala Met Glu Glu Leu 
    370                 375                 380                 


Leu Tyr Ser His Gly Leu Asp Ile Ala Phe Thr Gly His Val His Ala 
385                 390                 395                 400 


Tyr Glu Arg Ser Asn Arg Val Phe Asn Tyr Thr Leu Asp Pro Cys Gly 
                405                 410                 415     


Ala Val His Ile Ser Val Gly Asp Gly Gly Asn Arg Glu Lys Met Ala 
            420                 425                 430         


Thr Thr His Ala Asp Glu Pro Gly His Cys Pro Asp Pro Arg Pro Lys 
        435                 440                 445             


Pro Asn Ala Phe Ile Gly Gly Phe Cys Ala Ser Asn Phe Thr Ser Gly 
    450                 455                 460                 


Pro Ala Ala Gly Arg Phe Cys Trp Asp Arg Gln Pro Asp Tyr Ser Ala 
465                 470                 475                 480 


Tyr Arg Glu Ser Ser Phe Gly His Gly Ile Leu Glu Val Lys Asn Glu 
                485                 490                 495     


Thr His Ala Leu Trp Arg Trp His Arg Asn Gln Asp His Tyr Gly Ser 
            500                 505                 510         


Ala Gly Asp Glu Ile Tyr Ile Val Arg Glu Pro His Arg Cys Leu His 
        515                 520                 525             


Lys His Asn Ser Ser Arg Pro Ala His Gly Arg Ser Asn Thr Thr Arg 
    530                 535                 540                 


Glu Ser Gly Gly 
545             


<210>  19
<211>  1743
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  TaPAPhy_a2 Phytase cDNA
<222>  (1)..(1743)

<220>
<221>  CDS
<222>  (22)..(1665)
<223>  CDS encoding phytase

<400>  19
ctctcaatgc caagcaacac c atg tgg tgg ggg tcg ctg cgg ctg ctg ctg         51
                        Met Trp Trp Gly Ser Leu Arg Leu Leu Leu           
                        1               5                   10            

ctg ctc gcg gcg gcg gtg gcg gcg gct gct gag ccg gcg tcg acg ctc         99
Leu Leu Ala Ala Ala Val Ala Ala Ala Ala Glu Pro Ala Ser Thr Leu           
                15                  20                  25                

acc ggc ccg tcg cgg ccg gtg acg gtg gcg ctg cgg aaa gac agg ggc        147
Thr Gly Pro Ser Arg Pro Val Thr Val Ala Leu Arg Lys Asp Arg Gly           
            30                  35                  40                    

cac gcg gtg gac ctg ccg gac acg gac ccc cgg gtg cag cgc cgg gcc        195
His Ala Val Asp Leu Pro Asp Thr Asp Pro Arg Val Gln Arg Arg Ala           
        45                  50                  55                        

acg ggc tgg gct ccc gag cag atc acc gtc gcg ctc tcc gcc gct ccc        243
Thr Gly Trp Ala Pro Glu Gln Ile Thr Val Ala Leu Ser Ala Ala Pro           
    60                  65                  70                            

acc tct gcc tgg gtc tcc tgg atc acc ggc gaa ttc cag atg ggc ggc        291
Thr Ser Ala Trp Val Ser Trp Ile Thr Gly Glu Phe Gln Met Gly Gly           
75                  80                  85                  90            

acc gtc aag ccg ctg aac ccc ggc acg gtc gcc agc gtc gtg cgc tac        339
Thr Val Lys Pro Leu Asn Pro Gly Thr Val Ala Ser Val Val Arg Tyr           
                95                  100                 105               

ggg ctc gcc gcc gat tct ttg gtt cac gag gcc acc ggc gac gcg ctc        387
Gly Leu Ala Ala Asp Ser Leu Val His Glu Ala Thr Gly Asp Ala Leu           
            110                 115                 120                   

gtg tac agc cag ctc tac ccc ttc gag ggc ctc cag aac tac acc tcc        435
Val Tyr Ser Gln Leu Tyr Pro Phe Glu Gly Leu Gln Asn Tyr Thr Ser           
        125                 130                 135                       

ggc atc atc cac cac gtc cgc ctc caa ggg ctt gag cct gcg acg aag        483
Gly Ile Ile His His Val Arg Leu Gln Gly Leu Glu Pro Ala Thr Lys           
    140                 145                 150                           

tac tac tac cag tgc ggc gac ccg ggc atc ccg ggg gcg atg agc gcc        531
Tyr Tyr Tyr Gln Cys Gly Asp Pro Gly Ile Pro Gly Ala Met Ser Ala           
155                 160                 165                 170           

gtc cac gcg ttc cgg acg atg ccg gcg gtg ggg ccg cgg agc tac ccg        579
Val His Ala Phe Arg Thr Met Pro Ala Val Gly Pro Arg Ser Tyr Pro           
                175                 180                 185               

ggg agg atc gcc gtg gtg gga gac ctc ggg ctc acg tac aac acc acc        627
Gly Arg Ile Ala Val Val Gly Asp Leu Gly Leu Thr Tyr Asn Thr Thr           
            190                 195                 200                   

tcg acc gtg gac cac atg gtc agc aac cgg ccc gac ctg gtc ctc ctc        675
Ser Thr Val Asp His Met Val Ser Asn Arg Pro Asp Leu Val Leu Leu           
        205                 210                 215                       

gtc ggc gac gtg tgc tac gcc aac atg tac ctc acc aac ggc acc gga        723
Val Gly Asp Val Cys Tyr Ala Asn Met Tyr Leu Thr Asn Gly Thr Gly           
    220                 225                 230                           

gcg gac tgc tac tcg tgc gcg ttc ggc aag tcg acg ccc atc cac gag        771
Ala Asp Cys Tyr Ser Cys Ala Phe Gly Lys Ser Thr Pro Ile His Glu           
235                 240                 245                 250           

acg tac cag ccg cgc tgg gac tac tgg gga agg tac atg gag gcg gtg        819
Thr Tyr Gln Pro Arg Trp Asp Tyr Trp Gly Arg Tyr Met Glu Ala Val           
                255                 260                 265               

acg tcg ggc acg ccg atg atg gtg gtg gaa ggg aac cat gag ata gag        867
Thr Ser Gly Thr Pro Met Met Val Val Glu Gly Asn His Glu Ile Glu           
            270                 275                 280                   

gag cag atc ggc aac aag acg ttc gcg gcc tac cgc tcc cgg ttc gcg        915
Glu Gln Ile Gly Asn Lys Thr Phe Ala Ala Tyr Arg Ser Arg Phe Ala           
        285                 290                 295                       

ttc ccg tcg acg gag agc ggc tcc ttc tcc ccc ttc tac tac tcg ttc        963
Phe Pro Ser Thr Glu Ser Gly Ser Phe Ser Pro Phe Tyr Tyr Ser Phe           
    300                 305                 310                           

gac gcc ggc ggg atc cat ttc atc atg ctc gcc gcc tac gcc gat tac       1011
Asp Ala Gly Gly Ile His Phe Ile Met Leu Ala Ala Tyr Ala Asp Tyr           
315                 320                 325                 330           

agc agg tca ggg gag cag tac aga tgg ctg gtg aag gac ctg gcg aag       1059
Ser Arg Ser Gly Glu Gln Tyr Arg Trp Leu Val Lys Asp Leu Ala Lys           
                335                 340                 345               

gtg gac agg gcg gtg acc ccc tgg ctg gtc gcc ggc tgg cac gcg cca       1107
Val Asp Arg Ala Val Thr Pro Trp Leu Val Ala Gly Trp His Ala Pro           
            350                 355                 360                   

tgg tac acc acc tac aag gct cac tac agg gag gtg gag tgc atg aga       1155
Trp Tyr Thr Thr Tyr Lys Ala His Tyr Arg Glu Val Glu Cys Met Arg           
        365                 370                 375                       

gtg gcc atg gag gag ctg ctc tac tcc cac ggc ctc gac atc gcc ttc       1203
Val Ala Met Glu Glu Leu Leu Tyr Ser His Gly Leu Asp Ile Ala Phe           
    380                 385                 390                           

acc ggc cat gtg cac gcg tac gag cgc tcc aac cgg gtg ttc aac tac       1251
Thr Gly His Val His Ala Tyr Glu Arg Ser Asn Arg Val Phe Asn Tyr           
395                 400                 405                 410           

acg ctg gac ccg tgc ggc gcg gtg cac atc tcg gtg ggc gac ggc ggg       1299
Thr Leu Asp Pro Cys Gly Ala Val His Ile Ser Val Gly Asp Gly Gly           
                415                 420                 425               

aac cgg gag aag atg gcc acc acc cac gcc gac gag ccg ggg cac tgc       1347
Asn Arg Glu Lys Met Ala Thr Thr His Ala Asp Glu Pro Gly His Cys           
            430                 435                 440                   

ccg gac ccg cgg ccc aag ccc aac gcc ttc atc ggc tgc ttc tgc gcc       1395
Pro Asp Pro Arg Pro Lys Pro Asn Ala Phe Ile Gly Cys Phe Cys Ala           
        445                 450                 455                       

ttc aac ttc acg tcc ggc ccg gcc gcc ggc agg ttc tgc tgg gac cgg       1443
Phe Asn Phe Thr Ser Gly Pro Ala Ala Gly Arg Phe Cys Trp Asp Arg           
    460                 465                 470                           

cag ccg gac tac agc gcc tac cgg gag agc agc ttc ggc cac ggc atc       1491
Gln Pro Asp Tyr Ser Ala Tyr Arg Glu Ser Ser Phe Gly His Gly Ile           
475                 480                 485                 490           

ctc gag gtg aag aac gag acg cac gct ctg tgg aga tgg cac agg aac       1539
Leu Glu Val Lys Asn Glu Thr His Ala Leu Trp Arg Trp His Arg Asn           
                495                 500                 505               

cag gac cac tac gga agc gcc gga gat gag att tac att gtc cgg gag       1587
Gln Asp His Tyr Gly Ser Ala Gly Asp Glu Ile Tyr Ile Val Arg Glu           
            510                 515                 520                   

ccg cac agg tgc ttg cac aag cac aac tcg acc agg ccg gca cac ggt       1635
Pro His Arg Cys Leu His Lys His Asn Ser Thr Arg Pro Ala His Gly           
        525                 530                 535                       

cga caa aac acc aca cgg gaa tcg gga ggt taactgctgt actgctggag         1685
Arg Gln Asn Thr Thr Arg Glu Ser Gly Gly                                   
    540                 545                                               

tagatcgcgc ggtgtaatgg caactttata gatgattcgc ccaagcgtgg aaataaaa       1743


<210>  20
<211>  548
<212>  PRT
<213>  Triticum aestivum

<400>  20

Met Trp Trp Gly Ser Leu Arg Leu Leu Leu Leu Leu Ala Ala Ala Val 
1               5                   10                  15      


Ala Ala Ala Ala Glu Pro Ala Ser Thr Leu Thr Gly Pro Ser Arg Pro 
            20                  25                  30          


Val Thr Val Ala Leu Arg Lys Asp Arg Gly His Ala Val Asp Leu Pro 
        35                  40                  45              


Asp Thr Asp Pro Arg Val Gln Arg Arg Ala Thr Gly Trp Ala Pro Glu 
    50                  55                  60                  


Gln Ile Thr Val Ala Leu Ser Ala Ala Pro Thr Ser Ala Trp Val Ser 
65                  70                  75                  80  


Trp Ile Thr Gly Glu Phe Gln Met Gly Gly Thr Val Lys Pro Leu Asn 
                85                  90                  95      


Pro Gly Thr Val Ala Ser Val Val Arg Tyr Gly Leu Ala Ala Asp Ser 
            100                 105                 110         


Leu Val His Glu Ala Thr Gly Asp Ala Leu Val Tyr Ser Gln Leu Tyr 
        115                 120                 125             


Pro Phe Glu Gly Leu Gln Asn Tyr Thr Ser Gly Ile Ile His His Val 
    130                 135                 140                 


Arg Leu Gln Gly Leu Glu Pro Ala Thr Lys Tyr Tyr Tyr Gln Cys Gly 
145                 150                 155                 160 


Asp Pro Gly Ile Pro Gly Ala Met Ser Ala Val His Ala Phe Arg Thr 
                165                 170                 175     


Met Pro Ala Val Gly Pro Arg Ser Tyr Pro Gly Arg Ile Ala Val Val 
            180                 185                 190         


Gly Asp Leu Gly Leu Thr Tyr Asn Thr Thr Ser Thr Val Asp His Met 
        195                 200                 205             


Val Ser Asn Arg Pro Asp Leu Val Leu Leu Val Gly Asp Val Cys Tyr 
    210                 215                 220                 


Ala Asn Met Tyr Leu Thr Asn Gly Thr Gly Ala Asp Cys Tyr Ser Cys 
225                 230                 235                 240 


Ala Phe Gly Lys Ser Thr Pro Ile His Glu Thr Tyr Gln Pro Arg Trp 
                245                 250                 255     


Asp Tyr Trp Gly Arg Tyr Met Glu Ala Val Thr Ser Gly Thr Pro Met 
            260                 265                 270         


Met Val Val Glu Gly Asn His Glu Ile Glu Glu Gln Ile Gly Asn Lys 
        275                 280                 285             


Thr Phe Ala Ala Tyr Arg Ser Arg Phe Ala Phe Pro Ser Thr Glu Ser 
    290                 295                 300                 


Gly Ser Phe Ser Pro Phe Tyr Tyr Ser Phe Asp Ala Gly Gly Ile His 
305                 310                 315                 320 


Phe Ile Met Leu Ala Ala Tyr Ala Asp Tyr Ser Arg Ser Gly Glu Gln 
                325                 330                 335     


Tyr Arg Trp Leu Val Lys Asp Leu Ala Lys Val Asp Arg Ala Val Thr 
            340                 345                 350         


Pro Trp Leu Val Ala Gly Trp His Ala Pro Trp Tyr Thr Thr Tyr Lys 
        355                 360                 365             


Ala His Tyr Arg Glu Val Glu Cys Met Arg Val Ala Met Glu Glu Leu 
    370                 375                 380                 


Leu Tyr Ser His Gly Leu Asp Ile Ala Phe Thr Gly His Val His Ala 
385                 390                 395                 400 


Tyr Glu Arg Ser Asn Arg Val Phe Asn Tyr Thr Leu Asp Pro Cys Gly 
                405                 410                 415     


Ala Val His Ile Ser Val Gly Asp Gly Gly Asn Arg Glu Lys Met Ala 
            420                 425                 430         


Thr Thr His Ala Asp Glu Pro Gly His Cys Pro Asp Pro Arg Pro Lys 
        435                 440                 445             


Pro Asn Ala Phe Ile Gly Cys Phe Cys Ala Phe Asn Phe Thr Ser Gly 
    450                 455                 460                 


Pro Ala Ala Gly Arg Phe Cys Trp Asp Arg Gln Pro Asp Tyr Ser Ala 
465                 470                 475                 480 


Tyr Arg Glu Ser Ser Phe Gly His Gly Ile Leu Glu Val Lys Asn Glu 
                485                 490                 495     


Thr His Ala Leu Trp Arg Trp His Arg Asn Gln Asp His Tyr Gly Ser 
            500                 505                 510         


Ala Gly Asp Glu Ile Tyr Ile Val Arg Glu Pro His Arg Cys Leu His 
        515                 520                 525             


Lys His Asn Ser Thr Arg Pro Ala His Gly Arg Gln Asn Thr Thr Arg 
    530                 535                 540                 


Glu Ser Gly Gly 
545             


<210>  21
<211>  1734
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  TaPAPhy_a3 phytase cDNA
<222>  (1)..(1734)

<220>
<221>  CDS
<222>  (22)..(1638)
<223>  phytase coding sequence

<400>  21
ctgtcaatac caagcaacaa c atg tgg tgg ggg tcg ctg cgg ctg ctg ctg         51
                        Met Trp Trp Gly Ser Leu Arg Leu Leu Leu           
                        1               5                   10            

ctg ctc gcg gcg gcg gtg gcg gcg gct gct gag cca gcg tcg acg ctc         99
Leu Leu Ala Ala Ala Val Ala Ala Ala Ala Glu Pro Ala Ser Thr Leu           
                15                  20                  25                

acg ggg ccg tcg cgg ccg gtg acg gtg acg ctt cgg gaa gac agg ggc        147
Thr Gly Pro Ser Arg Pro Val Thr Val Thr Leu Arg Glu Asp Arg Gly           
            30                  35                  40                    

cac gcg gtg gac ctg ccg gac acg gac ccc cgg gtg cag cgc cgg gcc        195
His Ala Val Asp Leu Pro Asp Thr Asp Pro Arg Val Gln Arg Arg Ala           
        45                  50                  55                        

acg ggc tgg gct ccc gag cag atc gcc gtc gcg ctc tcc gcc gct ccc        243
Thr Gly Trp Ala Pro Glu Gln Ile Ala Val Ala Leu Ser Ala Ala Pro           
    60                  65                  70                            

acc tct gcc tgg gtc tcc tgg atc acc ggg gaa ttc cag atg ggc ggc        291
Thr Ser Ala Trp Val Ser Trp Ile Thr Gly Glu Phe Gln Met Gly Gly           
75                  80                  85                  90            

acc gtc aag ccg ctg gac ccc ggc acg gtc gcc agc gtc gtg cgc tac        339
Thr Val Lys Pro Leu Asp Pro Gly Thr Val Ala Ser Val Val Arg Tyr           
                95                  100                 105               

ggg ctc gcc gcc gat tct ttg gtt cgc cag gcc acc ggc gac gcg ctc        387
Gly Leu Ala Ala Asp Ser Leu Val Arg Gln Ala Thr Gly Asp Ala Leu           
            110                 115                 120                   

gtg tac agc cag ctc tac ccc ttc gag ggc ctc cag aac tac acc tcc        435
Val Tyr Ser Gln Leu Tyr Pro Phe Glu Gly Leu Gln Asn Tyr Thr Ser           
        125                 130                 135                       

ggc atc atc cac cac gtc cgc ctc caa ggg ctt gag cct gcg acg aag        483
Gly Ile Ile His His Val Arg Leu Gln Gly Leu Glu Pro Ala Thr Lys           
    140                 145                 150                           

tac tac tac cag tgt ggc gac ccg gcc ctc ccg ggg gcg atg agc gcc        531
Tyr Tyr Tyr Gln Cys Gly Asp Pro Ala Leu Pro Gly Ala Met Ser Ala           
155                 160                 165                 170           

gtc cac gcg ttc cgg acg atg ccg gcg gtg ggg ccg cgg agc tac ccg        579
Val His Ala Phe Arg Thr Met Pro Ala Val Gly Pro Arg Ser Tyr Pro           
                175                 180                 185               

ggg agg atc gcc gtg gtg gga gac ctc ggg ctc acg tac aac acc acg        627
Gly Arg Ile Ala Val Val Gly Asp Leu Gly Leu Thr Tyr Asn Thr Thr           
            190                 195                 200                   

tcg acc gtg gac cac atg gcg agc aac cgg ccg gac ctg gtc ctc ctc        675
Ser Thr Val Asp His Met Ala Ser Asn Arg Pro Asp Leu Val Leu Leu           
        205                 210                 215                       

ctc ggt gac gtc agc tac gcc aac ctg tac ctc acc aac ggc acc gga        723
Leu Gly Asp Val Ser Tyr Ala Asn Leu Tyr Leu Thr Asn Gly Thr Gly           
    220                 225                 230                           

gcg gac tgc tac tcg tgc gcg ttc ggc aag tcc acg ccc atc cac gag        771
Ala Asp Cys Tyr Ser Cys Ala Phe Gly Lys Ser Thr Pro Ile His Glu           
235                 240                 245                 250           

acg tac cag ccg cgc tgg gac tac tgg gga agg tac atg gag gcg gtg        819
Thr Tyr Gln Pro Arg Trp Asp Tyr Trp Gly Arg Tyr Met Glu Ala Val           
                255                 260                 265               

acg tcg ggg acg ccg atg gtg gtg gtg gag ggg aac cat gag ata gag        867
Thr Ser Gly Thr Pro Met Val Val Val Glu Gly Asn His Glu Ile Glu           
            270                 275                 280                   

gag cag atc ggc aac aag acg ttc gcg gcc tac cgc tcc cgg ttc gcg        915
Glu Gln Ile Gly Asn Lys Thr Phe Ala Ala Tyr Arg Ser Arg Phe Ala           
        285                 290                 295                       

ttc ccg tcg acg gag agc ggg tcc ttc tcc ccc ttc tac tac tcg ttc        963
Phe Pro Ser Thr Glu Ser Gly Ser Phe Ser Pro Phe Tyr Tyr Ser Phe           
    300                 305                 310                           

gac gcc ggg ggg atc cat ttc gtc atg ctc ggc gcc tac gcc gac tac       1011
Asp Ala Gly Gly Ile His Phe Val Met Leu Gly Ala Tyr Ala Asp Tyr           
315                 320                 325                 330           

ggc agg tca ggg gag cag tac aga tgg ctc gag aag gac ctg gcg aag       1059
Gly Arg Ser Gly Glu Gln Tyr Arg Trp Leu Glu Lys Asp Leu Ala Lys           
                335                 340                 345               

gtg gac agg tcg gtg acg ccg tgg ctg gtc gcc ggc tgg cac gcg cca       1107
Val Asp Arg Ser Val Thr Pro Trp Leu Val Ala Gly Trp His Ala Pro           
            350                 355                 360                   

tgg tac acc acc tat aag gct cac tac agg gag gtg gag tgc atg aga       1155
Trp Tyr Thr Thr Tyr Lys Ala His Tyr Arg Glu Val Glu Cys Met Arg           
        365                 370                 375                       

gtg gcc atg gag gag ctg ctc tac tcc cac ggc ctc gac atc gcc ttc       1203
Val Ala Met Glu Glu Leu Leu Tyr Ser His Gly Leu Asp Ile Ala Phe           
    380                 385                 390                           

acc ggc cat gtg cac gcg tac gag cgc tcc aac cgg gtg ttc aac tac       1251
Thr Gly His Val His Ala Tyr Glu Arg Ser Asn Arg Val Phe Asn Tyr           
395                 400                 405                 410           

acg ctg gac ccg tgc ggc gcc gtg cac atc tcg gtg ggc gac ggc ggg       1299
Thr Leu Asp Pro Cys Gly Ala Val His Ile Ser Val Gly Asp Gly Gly           
                415                 420                 425               

aac cgc gag aag atg gcc acc acc cac gcc gac gag ccg ggg cac tgc       1347
Asn Arg Glu Lys Met Ala Thr Thr His Ala Asp Glu Pro Gly His Cys           
            430                 435                 440                   

ccg gaa ccg cgg gcc aag ccc aac gcc ttc atc ggc ggc ttc tgc gcc       1395
Pro Glu Pro Arg Ala Lys Pro Asn Ala Phe Ile Gly Gly Phe Cys Ala           
        445                 450                 455                       

ttt aac ttc acg tcc ggc ccg gcc gcc ggc agg ttc tgc tgg gac cgg       1443
Phe Asn Phe Thr Ser Gly Pro Ala Ala Gly Arg Phe Cys Trp Asp Arg           
    460                 465                 470                           

cag ccg gac tac agc gcc tac cgg gag agc agc ttc ggc cac ggc atc       1491
Gln Pro Asp Tyr Ser Ala Tyr Arg Glu Ser Ser Phe Gly His Gly Ile           
475                 480                 485                 490           

ctc gag gtg aag aac gag acg cac gct ctg tgg aga tgg cac agg aac       1539
Leu Glu Val Lys Asn Glu Thr His Ala Leu Trp Arg Trp His Arg Asn           
                495                 500                 505               

cag gac atg tac ggg agc gcc gga gat gag att tac att gtc cgg gag       1587
Gln Asp Met Tyr Gly Ser Ala Gly Asp Glu Ile Tyr Ile Val Arg Glu           
            510                 515                 520                   

ccc cac agg tgc ttg cac aaa cac aac tcg acc agg ccg aca cac ggt       1635
Pro His Arg Cys Leu His Lys His Asn Ser Thr Arg Pro Thr His Gly           
        525                 530                 535                       

cga taaaacatca cacgggaatc tggaggtact actggagtaa acctcccggt            1688
Arg                                                                       
                                                                          

gtaataatgg caactattga cggttcgtcc aagcgtggaa ataaaa                    1734


<210>  22
<211>  539
<212>  PRT
<213>  Triticum aestivum

<400>  22

Met Trp Trp Gly Ser Leu Arg Leu Leu Leu Leu Leu Ala Ala Ala Val 
1               5                   10                  15      


Ala Ala Ala Ala Glu Pro Ala Ser Thr Leu Thr Gly Pro Ser Arg Pro 
            20                  25                  30          


Val Thr Val Thr Leu Arg Glu Asp Arg Gly His Ala Val Asp Leu Pro 
        35                  40                  45              


Asp Thr Asp Pro Arg Val Gln Arg Arg Ala Thr Gly Trp Ala Pro Glu 
    50                  55                  60                  


Gln Ile Ala Val Ala Leu Ser Ala Ala Pro Thr Ser Ala Trp Val Ser 
65                  70                  75                  80  


Trp Ile Thr Gly Glu Phe Gln Met Gly Gly Thr Val Lys Pro Leu Asp 
                85                  90                  95      


Pro Gly Thr Val Ala Ser Val Val Arg Tyr Gly Leu Ala Ala Asp Ser 
            100                 105                 110         


Leu Val Arg Gln Ala Thr Gly Asp Ala Leu Val Tyr Ser Gln Leu Tyr 
        115                 120                 125             


Pro Phe Glu Gly Leu Gln Asn Tyr Thr Ser Gly Ile Ile His His Val 
    130                 135                 140                 


Arg Leu Gln Gly Leu Glu Pro Ala Thr Lys Tyr Tyr Tyr Gln Cys Gly 
145                 150                 155                 160 


Asp Pro Ala Leu Pro Gly Ala Met Ser Ala Val His Ala Phe Arg Thr 
                165                 170                 175     


Met Pro Ala Val Gly Pro Arg Ser Tyr Pro Gly Arg Ile Ala Val Val 
            180                 185                 190         


Gly Asp Leu Gly Leu Thr Tyr Asn Thr Thr Ser Thr Val Asp His Met 
        195                 200                 205             


Ala Ser Asn Arg Pro Asp Leu Val Leu Leu Leu Gly Asp Val Ser Tyr 
    210                 215                 220                 


Ala Asn Leu Tyr Leu Thr Asn Gly Thr Gly Ala Asp Cys Tyr Ser Cys 
225                 230                 235                 240 


Ala Phe Gly Lys Ser Thr Pro Ile His Glu Thr Tyr Gln Pro Arg Trp 
                245                 250                 255     


Asp Tyr Trp Gly Arg Tyr Met Glu Ala Val Thr Ser Gly Thr Pro Met 
            260                 265                 270         


Val Val Val Glu Gly Asn His Glu Ile Glu Glu Gln Ile Gly Asn Lys 
        275                 280                 285             


Thr Phe Ala Ala Tyr Arg Ser Arg Phe Ala Phe Pro Ser Thr Glu Ser 
    290                 295                 300                 


Gly Ser Phe Ser Pro Phe Tyr Tyr Ser Phe Asp Ala Gly Gly Ile His 
305                 310                 315                 320 


Phe Val Met Leu Gly Ala Tyr Ala Asp Tyr Gly Arg Ser Gly Glu Gln 
                325                 330                 335     


Tyr Arg Trp Leu Glu Lys Asp Leu Ala Lys Val Asp Arg Ser Val Thr 
            340                 345                 350         


Pro Trp Leu Val Ala Gly Trp His Ala Pro Trp Tyr Thr Thr Tyr Lys 
        355                 360                 365             


Ala His Tyr Arg Glu Val Glu Cys Met Arg Val Ala Met Glu Glu Leu 
    370                 375                 380                 


Leu Tyr Ser His Gly Leu Asp Ile Ala Phe Thr Gly His Val His Ala 
385                 390                 395                 400 


Tyr Glu Arg Ser Asn Arg Val Phe Asn Tyr Thr Leu Asp Pro Cys Gly 
                405                 410                 415     


Ala Val His Ile Ser Val Gly Asp Gly Gly Asn Arg Glu Lys Met Ala 
            420                 425                 430         


Thr Thr His Ala Asp Glu Pro Gly His Cys Pro Glu Pro Arg Ala Lys 
        435                 440                 445             


Pro Asn Ala Phe Ile Gly Gly Phe Cys Ala Phe Asn Phe Thr Ser Gly 
    450                 455                 460                 


Pro Ala Ala Gly Arg Phe Cys Trp Asp Arg Gln Pro Asp Tyr Ser Ala 
465                 470                 475                 480 


Tyr Arg Glu Ser Ser Phe Gly His Gly Ile Leu Glu Val Lys Asn Glu 
                485                 490                 495     


Thr His Ala Leu Trp Arg Trp His Arg Asn Gln Asp Met Tyr Gly Ser 
            500                 505                 510         


Ala Gly Asp Glu Ile Tyr Ile Val Arg Glu Pro His Arg Cys Leu His 
        515                 520                 525             


Lys His Asn Ser Thr Arg Pro Thr His Gly Arg 
    530                 535                 


<210>  23
<211>  1730
<212>  DNA
<213>  Triticum monococcum


<220>
<221>  TmPAPhy_a1 phytase cDNA
<222>  (1)..(1730)

<220>
<221>  CDS
<222>  (17)..(1651)
<223>  Phytase coding sequence

<400>  23
aatgccaagc aacgcc atg tgg tgg ggg gcg ctg cag ctg ctg ctg ctg ctc       52
                  Met Trp Trp Gly Ala Leu Gln Leu Leu Leu Leu Leu         
                  1               5                   10                  

gtg gcg gcg gct gct gag ccg gcg tcg acg ctc acc ggc ccg tcg cgg        100
Val Ala Ala Ala Ala Glu Pro Ala Ser Thr Leu Thr Gly Pro Ser Arg           
        15                  20                  25                        

ccg gtg acg gtg gcg ctg cgg aaa gac agg ggc cac gcg gtg gac ctg        148
Pro Val Thr Val Ala Leu Arg Lys Asp Arg Gly His Ala Val Asp Leu           
    30                  35                  40                            

ccg gac acg gac ccc cgg gtg cag cgc cgg gcc acg ggc tgg gct ccc        196
Pro Asp Thr Asp Pro Arg Val Gln Arg Arg Ala Thr Gly Trp Ala Pro           
45                  50                  55                  60            

gag cag atc acc gtc gcg ctc tcc gcc gct ccc acc tct gcc tgg gtc        244
Glu Gln Ile Thr Val Ala Leu Ser Ala Ala Pro Thr Ser Ala Trp Val           
                65                  70                  75                

tcc tgg atc acc ggg gaa ttc cag atg ggc ggc aca gtc aag ccg ctg        292
Ser Trp Ile Thr Gly Glu Phe Gln Met Gly Gly Thr Val Lys Pro Leu           
            80                  85                  90                    

cac ccc ggc acg gtc gcc agc gtc gtg cgc tac ggg ctc gcc gcc gat        340
His Pro Gly Thr Val Ala Ser Val Val Arg Tyr Gly Leu Ala Ala Asp           
        95                  100                 105                       

tct ttg gtt cgc gag gcc acc ggc gac gcg ctt gtg tac agc cag ctc        388
Ser Leu Val Arg Glu Ala Thr Gly Asp Ala Leu Val Tyr Ser Gln Leu           
    110                 115                 120                           

tac ccc ttc gag ggc ctc cag aac tac acc tcc ggc atc atc cac cac        436
Tyr Pro Phe Glu Gly Leu Gln Asn Tyr Thr Ser Gly Ile Ile His His           
125                 130                 135                 140           

gtc cgc ctc caa ggg ctt gag cct gcg acg aag tac tac tac cag tgc        484
Val Arg Leu Gln Gly Leu Glu Pro Ala Thr Lys Tyr Tyr Tyr Gln Cys           
                145                 150                 155               

ggc gac ccg ggc atc ccg ggg gcg atg agc gcc gtc cac gcg ttc cgg        532
Gly Asp Pro Gly Ile Pro Gly Ala Met Ser Ala Val His Ala Phe Arg           
            160                 165                 170                   

acg atg ccg gcg gtg ggg ccg cgg agc tac ccg ggg agg atc gcc gtg        580
Thr Met Pro Ala Val Gly Pro Arg Ser Tyr Pro Gly Arg Ile Ala Val           
        175                 180                 185                       

gtg gga gac ctc ggg ctc acg tac aac acc acc tcc acc gtg gac cac        628
Val Gly Asp Leu Gly Leu Thr Tyr Asn Thr Thr Ser Thr Val Asp His           
    190                 195                 200                           

atg gtc agc aac cgg ccg gac ctg gtc ctc ctc gtc ggc gac gtg tgc        676
Met Val Ser Asn Arg Pro Asp Leu Val Leu Leu Val Gly Asp Val Cys           
205                 210                 215                 220           

tac gcc aac atg tac ctc acc aac ggc acc gga gcg gac tgc tac tcg        724
Tyr Ala Asn Met Tyr Leu Thr Asn Gly Thr Gly Ala Asp Cys Tyr Ser           
                225                 230                 235               

tgc gcg ttc ggc aag tcg acg ccc atc cac gag acg tac cag ccg cgc        772
Cys Ala Phe Gly Lys Ser Thr Pro Ile His Glu Thr Tyr Gln Pro Arg           
            240                 245                 250                   

tgg gac tac tgg gga agg tac atg gag gcg gtg acg tcg ggg acg ccg        820
Trp Asp Tyr Trp Gly Arg Tyr Met Glu Ala Val Thr Ser Gly Thr Pro           
        255                 260                 265                       

atg atg gtg gtg gaa ggg aac cat gag atc gag gag cag atc cgc aac        868
Met Met Val Val Glu Gly Asn His Glu Ile Glu Glu Gln Ile Arg Asn           
    270                 275                 280                           

agg acg ttc gcg gcc tac cgc tcc cgg ttc gcg ttc ccg tcg acg gag        916
Arg Thr Phe Ala Ala Tyr Arg Ser Arg Phe Ala Phe Pro Ser Thr Glu           
285                 290                 295                 300           

agc ggc tcc ttc tcc ccc ttc tac tac tcc ttc gac gcc ggc ggg atc        964
Ser Gly Ser Phe Ser Pro Phe Tyr Tyr Ser Phe Asp Ala Gly Gly Ile           
                305                 310                 315               

cat ttc gtc atg ctc gcc gcg tac gcc gac tac agc agg tca ggg gag       1012
His Phe Val Met Leu Ala Ala Tyr Ala Asp Tyr Ser Arg Ser Gly Glu           
            320                 325                 330                   

cag tac aga tgg ctg aag aag gac ctg gcg aag gtg gac agg gcg gtg       1060
Gln Tyr Arg Trp Leu Lys Lys Asp Leu Ala Lys Val Asp Arg Ala Val           
        335                 340                 345                       

acc ccc tgg ctg gtc gcc ggc tgg cac gcg cca tgg tac acc acc tac       1108
Thr Pro Trp Leu Val Ala Gly Trp His Ala Pro Trp Tyr Thr Thr Tyr           
    350                 355                 360                           

aag gct cac tac agg gag gtg gag tgc atg aga gtg gcc atg gag gag       1156
Lys Ala His Tyr Arg Glu Val Glu Cys Met Arg Val Ala Met Glu Glu           
365                 370                 375                 380           

ctg ctc tac tcc cac ggc ctc gac atc gcc ttc acc ggc cat gtg cac       1204
Leu Leu Tyr Ser His Gly Leu Asp Ile Ala Phe Thr Gly His Val His           
                385                 390                 395               

gcg tac gag cgc tcc aac cgg gtg ttc aac tac acg ctg gac ccg tgc       1252
Ala Tyr Glu Arg Ser Asn Arg Val Phe Asn Tyr Thr Leu Asp Pro Cys           
            400                 405                 410                   

ggc gcg gtg cac atc tcg gtg ggc gac ggc ggg aac cgg gag aag atg       1300
Gly Ala Val His Ile Ser Val Gly Asp Gly Gly Asn Arg Glu Lys Met           
        415                 420                 425                       

gcc acc acc cac gcc gac gag ccg ggg cac tgc ccg gac ccg cgg ccc       1348
Ala Thr Thr His Ala Asp Glu Pro Gly His Cys Pro Asp Pro Arg Pro           
    430                 435                 440                           

aag ccc aac gcc ttc atc ggc ggc ttc tgc gcc tcc aac ttc acg tcc       1396
Lys Pro Asn Ala Phe Ile Gly Gly Phe Cys Ala Ser Asn Phe Thr Ser           
445                 450                 455                 460           

ggc ccg gcc gcc ggc agg ttc tgc tgg gac cgg cag ccg gac tac agc       1444
Gly Pro Ala Ala Gly Arg Phe Cys Trp Asp Arg Gln Pro Asp Tyr Ser           
                465                 470                 475               

gcc tac cgg gaa agc agc ttc ggc cac ggc atc ctc gag gtg aag aac       1492
Ala Tyr Arg Glu Ser Ser Phe Gly His Gly Ile Leu Glu Val Lys Asn           
            480                 485                 490                   

gag acg cac gct ctg tgg aga tgg cac agg aac cag gac cac tac gga       1540
Glu Thr His Ala Leu Trp Arg Trp His Arg Asn Gln Asp His Tyr Gly           
        495                 500                 505                       

agc gcc gga gat gag att tac att gtc cgg gag ccg cac agg tgc ttg       1588
Ser Ala Gly Asp Glu Ile Tyr Ile Val Arg Glu Pro His Arg Cys Leu           
    510                 515                 520                           

cac aag cac aac tcg acc agg ccg gca cac ggt cga caa aac acc aca       1636
His Lys His Asn Ser Thr Arg Pro Ala His Gly Arg Gln Asn Thr Thr           
525                 530                 535                 540           

cgg gaa tcg gga ggc taactgctgt actgctggag tagatcgcgc ggtgtaatgg       1691
Arg Glu Ser Gly Gly                                                       
                545                                                       

caactatata gacggttcgc ccaagcgtgg aaataaaaa                            1730


<210>  24
<211>  545
<212>  PRT
<213>  Triticum monococcum

<400>  24

Met Trp Trp Gly Ala Leu Gln Leu Leu Leu Leu Leu Val Ala Ala Ala 
1               5                   10                  15      


Ala Glu Pro Ala Ser Thr Leu Thr Gly Pro Ser Arg Pro Val Thr Val 
            20                  25                  30          


Ala Leu Arg Lys Asp Arg Gly His Ala Val Asp Leu Pro Asp Thr Asp 
        35                  40                  45              


Pro Arg Val Gln Arg Arg Ala Thr Gly Trp Ala Pro Glu Gln Ile Thr 
    50                  55                  60                  


Val Ala Leu Ser Ala Ala Pro Thr Ser Ala Trp Val Ser Trp Ile Thr 
65                  70                  75                  80  


Gly Glu Phe Gln Met Gly Gly Thr Val Lys Pro Leu His Pro Gly Thr 
                85                  90                  95      


Val Ala Ser Val Val Arg Tyr Gly Leu Ala Ala Asp Ser Leu Val Arg 
            100                 105                 110         


Glu Ala Thr Gly Asp Ala Leu Val Tyr Ser Gln Leu Tyr Pro Phe Glu 
        115                 120                 125             


Gly Leu Gln Asn Tyr Thr Ser Gly Ile Ile His His Val Arg Leu Gln 
    130                 135                 140                 


Gly Leu Glu Pro Ala Thr Lys Tyr Tyr Tyr Gln Cys Gly Asp Pro Gly 
145                 150                 155                 160 


Ile Pro Gly Ala Met Ser Ala Val His Ala Phe Arg Thr Met Pro Ala 
                165                 170                 175     


Val Gly Pro Arg Ser Tyr Pro Gly Arg Ile Ala Val Val Gly Asp Leu 
            180                 185                 190         


Gly Leu Thr Tyr Asn Thr Thr Ser Thr Val Asp His Met Val Ser Asn 
        195                 200                 205             


Arg Pro Asp Leu Val Leu Leu Val Gly Asp Val Cys Tyr Ala Asn Met 
    210                 215                 220                 


Tyr Leu Thr Asn Gly Thr Gly Ala Asp Cys Tyr Ser Cys Ala Phe Gly 
225                 230                 235                 240 


Lys Ser Thr Pro Ile His Glu Thr Tyr Gln Pro Arg Trp Asp Tyr Trp 
                245                 250                 255     


Gly Arg Tyr Met Glu Ala Val Thr Ser Gly Thr Pro Met Met Val Val 
            260                 265                 270         


Glu Gly Asn His Glu Ile Glu Glu Gln Ile Arg Asn Arg Thr Phe Ala 
        275                 280                 285             


Ala Tyr Arg Ser Arg Phe Ala Phe Pro Ser Thr Glu Ser Gly Ser Phe 
    290                 295                 300                 


Ser Pro Phe Tyr Tyr Ser Phe Asp Ala Gly Gly Ile His Phe Val Met 
305                 310                 315                 320 


Leu Ala Ala Tyr Ala Asp Tyr Ser Arg Ser Gly Glu Gln Tyr Arg Trp 
                325                 330                 335     


Leu Lys Lys Asp Leu Ala Lys Val Asp Arg Ala Val Thr Pro Trp Leu 
            340                 345                 350         


Val Ala Gly Trp His Ala Pro Trp Tyr Thr Thr Tyr Lys Ala His Tyr 
        355                 360                 365             


Arg Glu Val Glu Cys Met Arg Val Ala Met Glu Glu Leu Leu Tyr Ser 
    370                 375                 380                 


His Gly Leu Asp Ile Ala Phe Thr Gly His Val His Ala Tyr Glu Arg 
385                 390                 395                 400 


Ser Asn Arg Val Phe Asn Tyr Thr Leu Asp Pro Cys Gly Ala Val His 
                405                 410                 415     


Ile Ser Val Gly Asp Gly Gly Asn Arg Glu Lys Met Ala Thr Thr His 
            420                 425                 430         


Ala Asp Glu Pro Gly His Cys Pro Asp Pro Arg Pro Lys Pro Asn Ala 
        435                 440                 445             


Phe Ile Gly Gly Phe Cys Ala Ser Asn Phe Thr Ser Gly Pro Ala Ala 
    450                 455                 460                 


Gly Arg Phe Cys Trp Asp Arg Gln Pro Asp Tyr Ser Ala Tyr Arg Glu 
465                 470                 475                 480 


Ser Ser Phe Gly His Gly Ile Leu Glu Val Lys Asn Glu Thr His Ala 
                485                 490                 495     


Leu Trp Arg Trp His Arg Asn Gln Asp His Tyr Gly Ser Ala Gly Asp 
            500                 505                 510         


Glu Ile Tyr Ile Val Arg Glu Pro His Arg Cys Leu His Lys His Asn 
        515                 520                 525             


Ser Thr Arg Pro Ala His Gly Arg Gln Asn Thr Thr Arg Glu Ser Gly 
    530                 535                 540                 


Gly 
545 


<210>  25
<211>  1744
<212>  DNA
<213>  Secale cereale


<220>
<221>  ScPAPhy_a1 phytase cDNA
<222>  (1)..(1744)

<220>
<221>  CDS
<222>  (17)..(1639)

<400>  25
aatgccaagc aacaac atg tgg cgg ggg tcg ctg cgg ctg ctg ctg ctg ctc       52
                  Met Trp Arg Gly Ser Leu Arg Leu Leu Leu Leu Leu         
                  1               5                   10                  

gcg gcg gcg gtg acg gcg gct gct gag ccg ggg tcg acg ctc atg ggc        100
Ala Ala Ala Val Thr Ala Ala Ala Glu Pro Gly Ser Thr Leu Met Gly           
        15                  20                  25                        

ccg tca cgg ccg gtt acg gtg gcg ctg cgg gaa gac agg ggc cac gcg        148
Pro Ser Arg Pro Val Thr Val Ala Leu Arg Glu Asp Arg Gly His Ala           
    30                  35                  40                            

gtg gac ctg ccg gac acg gac ccg cgg gtg cag cgc cgg gca aat ggc        196
Val Asp Leu Pro Asp Thr Asp Pro Arg Val Gln Arg Arg Ala Asn Gly           
45                  50                  55                  60            

tgg gct cct gag cag atc gcc gtc gcg ctc tcc gct gct ccc acc tct        244
Trp Ala Pro Glu Gln Ile Ala Val Ala Leu Ser Ala Ala Pro Thr Ser           
                65                  70                  75                

gcc tgg gtc tcc tgg atc aca ggg gaa ttc cag atg ggc ggc acc gtc        292
Ala Trp Val Ser Trp Ile Thr Gly Glu Phe Gln Met Gly Gly Thr Val           
            80                  85                  90                    

aag ccg ctg gac ccc ggc acg gtc ggt agc gtc gtg cgc tac ggg ctc        340
Lys Pro Leu Asp Pro Gly Thr Val Gly Ser Val Val Arg Tyr Gly Leu           
        95                  100                 105                       

gcc gcc gat tct ttg gtt cgt gtg gcc acc ggc gac gcg ctc gtg tac        388
Ala Ala Asp Ser Leu Val Arg Val Ala Thr Gly Asp Ala Leu Val Tyr           
    110                 115                 120                           

agc cag ctc tac cca ttc gag ggc ctc cag aac tac acc tcc ggc atc        436
Ser Gln Leu Tyr Pro Phe Glu Gly Leu Gln Asn Tyr Thr Ser Gly Ile           
125                 130                 135                 140           

atc cac cac gtc cgc ctc caa ggg ctt gag cct ggg acg aag tac tac        484
Ile His His Val Arg Leu Gln Gly Leu Glu Pro Gly Thr Lys Tyr Tyr           
                145                 150                 155               

tac cag tgc ggc gac ccg gcc ctc ccg ggg gcg atg agc gcc gtc cac        532
Tyr Gln Cys Gly Asp Pro Ala Leu Pro Gly Ala Met Ser Ala Val His           
            160                 165                 170                   

gcg ttc cgg acg atg ccg gcg gtg ggg ccg cgg agc tac ccg ggg agg        580
Ala Phe Arg Thr Met Pro Ala Val Gly Pro Arg Ser Tyr Pro Gly Arg           
        175                 180                 185                       

atc gcc gtg gtg gga gac ctc ggg ctc acg tac aac acc acc tcc acc        628
Ile Ala Val Val Gly Asp Leu Gly Leu Thr Tyr Asn Thr Thr Ser Thr           
    190                 195                 200                           

gtg gac cac atg gtg agc aac cgg ccg gac ctg gtg gtc ctc gtc ggc        676
Val Asp His Met Val Ser Asn Arg Pro Asp Leu Val Val Leu Val Gly           
205                 210                 215                 220           

gac gtg agc tac gcc aac ctg tac ctc acc aac ggc acc gga gcg gac        724
Asp Val Ser Tyr Ala Asn Leu Tyr Leu Thr Asn Gly Thr Gly Ala Asp           
                225                 230                 235               

tgc tac tcg tgc gcg ttc ggc aag tcg acg ccc atc cac gag acg tac        772
Cys Tyr Ser Cys Ala Phe Gly Lys Ser Thr Pro Ile His Glu Thr Tyr           
            240                 245                 250                   

cag ccg cgc tgg gac tac tgg ggg agg tac atg gag gcg gtg acg tcg        820
Gln Pro Arg Trp Asp Tyr Trp Gly Arg Tyr Met Glu Ala Val Thr Ser           
        255                 260                 265                       

ggg acg ccg atg atg gtg gtg gag ggg aac cat gag ata gag gag cag        868
Gly Thr Pro Met Met Val Val Glu Gly Asn His Glu Ile Glu Glu Gln           
    270                 275                 280                           

atc ggt aaa aag acg ttc gag gcg tac cgc tcc cgg ttc gcg ttc ccg        916
Ile Gly Lys Lys Thr Phe Glu Ala Tyr Arg Ser Arg Phe Ala Phe Pro           
285                 290                 295                 300           

tcg gcg gag agc ggg tcc ttc tcc ccc ttc tac tac tcc ttc gac gcc        964
Ser Ala Glu Ser Gly Ser Phe Ser Pro Phe Tyr Tyr Ser Phe Asp Ala           
                305                 310                 315               

ggc ggg atc cat ttc atc atg ctc gcc gcc tac gac gac tac agc agg       1012
Gly Gly Ile His Phe Ile Met Leu Ala Ala Tyr Asp Asp Tyr Ser Arg           
            320                 325                 330                   

tca gga gag cag tac cga tgg ctg gag aag gac ctg tcg aag gtg gac       1060
Ser Gly Glu Gln Tyr Arg Trp Leu Glu Lys Asp Leu Ser Lys Val Asp           
        335                 340                 345                       

agg tcg gtg acg ccg tgg ctg gtc gcc ggc tgg cac gcg cca tgg tac       1108
Arg Ser Val Thr Pro Trp Leu Val Ala Gly Trp His Ala Pro Trp Tyr           
    350                 355                 360                           

acc acc tac aag gct cac tac agg gag gtg gag tgc atg aga gtg tcc       1156
Thr Thr Tyr Lys Ala His Tyr Arg Glu Val Glu Cys Met Arg Val Ser           
365                 370                 375                 380           

atg gag gag ctg ctc tac tcc cac ggc ctc gac atc gcc ttc acc ggc       1204
Met Glu Glu Leu Leu Tyr Ser His Gly Leu Asp Ile Ala Phe Thr Gly           
                385                 390                 395               

cat gtg cac gcg tac gag cgc tcc aac cgg gtg ttc aac tac acg ctg       1252
His Val His Ala Tyr Glu Arg Ser Asn Arg Val Phe Asn Tyr Thr Leu           
            400                 405                 410                   

gac ccg tgc ggt gcc gtg cac atc tcg gtg ggc gac ggc ggg aac cgc       1300
Asp Pro Cys Gly Ala Val His Ile Ser Val Gly Asp Gly Gly Asn Arg           
        415                 420                 425                       

gag aag atg gcc acc acc cac gcc gac gag ccg ggg cac tgc ccg gac       1348
Glu Lys Met Ala Thr Thr His Ala Asp Glu Pro Gly His Cys Pro Asp           
    430                 435                 440                           

ccg cgg ccc aag ccc aac gcc ttc atc ggc ggc ttc tgc ggc ttt aac       1396
Pro Arg Pro Lys Pro Asn Ala Phe Ile Gly Gly Phe Cys Gly Phe Asn           
445                 450                 455                 460           

ttc acg tcc ggc ccg gcc gcc gga agg tac tgc tgg gac cgg cag ccg       1444
Phe Thr Ser Gly Pro Ala Ala Gly Arg Tyr Cys Trp Asp Arg Gln Pro           
                465                 470                 475               

gac tac agc gcc tac cgg gag agc agc ttt ggc cac ggc atc ctc gag       1492
Asp Tyr Ser Ala Tyr Arg Glu Ser Ser Phe Gly His Gly Ile Leu Glu           
            480                 485                 490                   

gtg aag aac gag acg cac gct ctg tgg aga tgg cac agg aac cag gac       1540
Val Lys Asn Glu Thr His Ala Leu Trp Arg Trp His Arg Asn Gln Asp           
        495                 500                 505                       

atg tac ggg agc gcc gga gat gag att tac att gtc cgg gag ccg gag       1588
Met Tyr Gly Ser Ala Gly Asp Glu Ile Tyr Ile Val Arg Glu Pro Glu           
    510                 515                 520                           

agg tgc ttg cac aag cac aag cac aac tcg acc agg ccg gca cac ggc       1636
Arg Cys Leu His Lys His Lys His Asn Ser Thr Arg Pro Ala His Gly           
525                 530                 535                 540           

cga taaacaccac gcgggaatcg ggaggttaac tgctgtactg ctggagtaga            1689
Arg                                                                       
                                                                          

tcgcgcggtg taatgacaac tatatagacg gttcgccaaa gcgtggaaat aaaaa          1744


<210>  26
<211>  541
<212>  PRT
<213>  Secale cereale

<400>  26

Met Trp Arg Gly Ser Leu Arg Leu Leu Leu Leu Leu Ala Ala Ala Val 
1               5                   10                  15      


Thr Ala Ala Ala Glu Pro Gly Ser Thr Leu Met Gly Pro Ser Arg Pro 
            20                  25                  30          


Val Thr Val Ala Leu Arg Glu Asp Arg Gly His Ala Val Asp Leu Pro 
        35                  40                  45              


Asp Thr Asp Pro Arg Val Gln Arg Arg Ala Asn Gly Trp Ala Pro Glu 
    50                  55                  60                  


Gln Ile Ala Val Ala Leu Ser Ala Ala Pro Thr Ser Ala Trp Val Ser 
65                  70                  75                  80  


Trp Ile Thr Gly Glu Phe Gln Met Gly Gly Thr Val Lys Pro Leu Asp 
                85                  90                  95      


Pro Gly Thr Val Gly Ser Val Val Arg Tyr Gly Leu Ala Ala Asp Ser 
            100                 105                 110         


Leu Val Arg Val Ala Thr Gly Asp Ala Leu Val Tyr Ser Gln Leu Tyr 
        115                 120                 125             


Pro Phe Glu Gly Leu Gln Asn Tyr Thr Ser Gly Ile Ile His His Val 
    130                 135                 140                 


Arg Leu Gln Gly Leu Glu Pro Gly Thr Lys Tyr Tyr Tyr Gln Cys Gly 
145                 150                 155                 160 


Asp Pro Ala Leu Pro Gly Ala Met Ser Ala Val His Ala Phe Arg Thr 
                165                 170                 175     


Met Pro Ala Val Gly Pro Arg Ser Tyr Pro Gly Arg Ile Ala Val Val 
            180                 185                 190         


Gly Asp Leu Gly Leu Thr Tyr Asn Thr Thr Ser Thr Val Asp His Met 
        195                 200                 205             


Val Ser Asn Arg Pro Asp Leu Val Val Leu Val Gly Asp Val Ser Tyr 
    210                 215                 220                 


Ala Asn Leu Tyr Leu Thr Asn Gly Thr Gly Ala Asp Cys Tyr Ser Cys 
225                 230                 235                 240 


Ala Phe Gly Lys Ser Thr Pro Ile His Glu Thr Tyr Gln Pro Arg Trp 
                245                 250                 255     


Asp Tyr Trp Gly Arg Tyr Met Glu Ala Val Thr Ser Gly Thr Pro Met 
            260                 265                 270         


Met Val Val Glu Gly Asn His Glu Ile Glu Glu Gln Ile Gly Lys Lys 
        275                 280                 285             


Thr Phe Glu Ala Tyr Arg Ser Arg Phe Ala Phe Pro Ser Ala Glu Ser 
    290                 295                 300                 


Gly Ser Phe Ser Pro Phe Tyr Tyr Ser Phe Asp Ala Gly Gly Ile His 
305                 310                 315                 320 


Phe Ile Met Leu Ala Ala Tyr Asp Asp Tyr Ser Arg Ser Gly Glu Gln 
                325                 330                 335     


Tyr Arg Trp Leu Glu Lys Asp Leu Ser Lys Val Asp Arg Ser Val Thr 
            340                 345                 350         


Pro Trp Leu Val Ala Gly Trp His Ala Pro Trp Tyr Thr Thr Tyr Lys 
        355                 360                 365             


Ala His Tyr Arg Glu Val Glu Cys Met Arg Val Ser Met Glu Glu Leu 
    370                 375                 380                 


Leu Tyr Ser His Gly Leu Asp Ile Ala Phe Thr Gly His Val His Ala 
385                 390                 395                 400 


Tyr Glu Arg Ser Asn Arg Val Phe Asn Tyr Thr Leu Asp Pro Cys Gly 
                405                 410                 415     


Ala Val His Ile Ser Val Gly Asp Gly Gly Asn Arg Glu Lys Met Ala 
            420                 425                 430         


Thr Thr His Ala Asp Glu Pro Gly His Cys Pro Asp Pro Arg Pro Lys 
        435                 440                 445             


Pro Asn Ala Phe Ile Gly Gly Phe Cys Gly Phe Asn Phe Thr Ser Gly 
    450                 455                 460                 


Pro Ala Ala Gly Arg Tyr Cys Trp Asp Arg Gln Pro Asp Tyr Ser Ala 
465                 470                 475                 480 


Tyr Arg Glu Ser Ser Phe Gly His Gly Ile Leu Glu Val Lys Asn Glu 
                485                 490                 495     


Thr His Ala Leu Trp Arg Trp His Arg Asn Gln Asp Met Tyr Gly Ser 
            500                 505                 510         


Ala Gly Asp Glu Ile Tyr Ile Val Arg Glu Pro Glu Arg Cys Leu His 
        515                 520                 525             


Lys His Lys His Asn Ser Thr Arg Pro Ala His Gly Arg 
    530                 535                 540     


<210>  27
<211>  1734
<212>  DNA
<213>  Secale cereale


<220>
<221>  ScPAPhy_a2 phytase cDNA
<222>  (1)..(1734)

<220>
<221>  CDS
<222>  (14)..(1630)
<223>  Phytase coding sequence

<400>  27
aatgccaagc aac atg tgg ctg ggg tcg ctg cgg ctg ctg ctg ctg ctc          49
               Met Trp Leu Gly Ser Leu Arg Leu Leu Leu Leu Leu            
               1               5                   10                     

gcg gcg gcg gtg acg gcg gct gct gag ccg gcg tcc acg ctc atg ggc         97
Ala Ala Ala Val Thr Ala Ala Ala Glu Pro Ala Ser Thr Leu Met Gly           
        15                  20                  25                        

ccg tca cgg ccg gtt acg gtg gcg ctg cgg gaa gac agg ggc cac gcg        145
Pro Ser Arg Pro Val Thr Val Ala Leu Arg Glu Asp Arg Gly His Ala           
    30                  35                  40                            

gtg gac ctg ccg gac acg gac ccg cgg gtg cag cgc cgg gca aat ggc        193
Val Asp Leu Pro Asp Thr Asp Pro Arg Val Gln Arg Arg Ala Asn Gly           
45                  50                  55                  60            

tgg gct cct gag cag atc gcc gtc gcg ctc tcc gct gct ccc acc tct        241
Trp Ala Pro Glu Gln Ile Ala Val Ala Leu Ser Ala Ala Pro Thr Ser           
                65                  70                  75                

gcc tgg gtc tcc tgg atc acc ggg gaa ttc cag atg ggt ggc acc gtc        289
Ala Trp Val Ser Trp Ile Thr Gly Glu Phe Gln Met Gly Gly Thr Val           
            80                  85                  90                    

aag ccg ctg gac ccc ggc acg gtc ggt agc gtc gtg cgc tac gga ctc        337
Lys Pro Leu Asp Pro Gly Thr Val Gly Ser Val Val Arg Tyr Gly Leu           
        95                  100                 105                       

gcc gcc gat tct ttg gtt cgc gtg gcc acc ggc gac gcg ctc gtg tac        385
Ala Ala Asp Ser Leu Val Arg Val Ala Thr Gly Asp Ala Leu Val Tyr           
    110                 115                 120                           

agc cag ctc tac ccc ttc gag ggc ctc cag aac tac acc tcc ggc atc        433
Ser Gln Leu Tyr Pro Phe Glu Gly Leu Gln Asn Tyr Thr Ser Gly Ile           
125                 130                 135                 140           

atc cac cac gtc cgc ctc caa ggg ctt gag cct ggg acg aag tac tac        481
Ile His His Val Arg Leu Gln Gly Leu Glu Pro Gly Thr Lys Tyr Tyr           
                145                 150                 155               

tac cag tgc ggc gac ccg gcc ctc ccg ggg acg atg agc gcc gtc cac        529
Tyr Gln Cys Gly Asp Pro Ala Leu Pro Gly Thr Met Ser Ala Val His           
            160                 165                 170                   

gcg ttc cgg acg atg ccg gcg gtc ggg ccg cgg agc tac ccg ggg agg        577
Ala Phe Arg Thr Met Pro Ala Val Gly Pro Arg Ser Tyr Pro Gly Arg           
        175                 180                 185                       

atc gcc gtg gtg gga gac ctc ggg ctc acg tac aac acc acc tcc acc        625
Ile Ala Val Val Gly Asp Leu Gly Leu Thr Tyr Asn Thr Thr Ser Thr           
    190                 195                 200                           

gtg gac cac atg atg agc aac cgg ccg gat ctg gtc gtc ctc gtc ggc        673
Val Asp His Met Met Ser Asn Arg Pro Asp Leu Val Val Leu Val Gly           
205                 210                 215                 220           

gac gtg agc tac gcc aac ctg tac ctc acc aac ggc acc gga gcg gac        721
Asp Val Ser Tyr Ala Asn Leu Tyr Leu Thr Asn Gly Thr Gly Ala Asp           
                225                 230                 235               

tgc tac tcg tgc gcg ttc ggc aag tcg acg ccc atc cac gag acg tac        769
Cys Tyr Ser Cys Ala Phe Gly Lys Ser Thr Pro Ile His Glu Thr Tyr           
            240                 245                 250                   

cag ccg cgc tgg gac tac tgg gga agg tac atg gag gcg gtg acg tcg        817
Gln Pro Arg Trp Asp Tyr Trp Gly Arg Tyr Met Glu Ala Val Thr Ser           
        255                 260                 265                       

ggc acg ccg atg atg gtg gtg gag ggg aac cat gag ata gag gag cag        865
Gly Thr Pro Met Met Val Val Glu Gly Asn His Glu Ile Glu Glu Gln           
    270                 275                 280                           

atc ggc aaa aag acg ttc gag gcg tac cgc tcc cgg ttc gcg ttt ccg        913
Ile Gly Lys Lys Thr Phe Glu Ala Tyr Arg Ser Arg Phe Ala Phe Pro           
285                 290                 295                 300           

tcg gcg gag aac ggg tcc ttc tcc ccc ttc tac tac tcc ttc gac gcc        961
Ser Ala Glu Asn Gly Ser Phe Ser Pro Phe Tyr Tyr Ser Phe Asp Ala           
                305                 310                 315               

ggc ggg atc cat ttc atc atg ctc gcc gcc tac gcc gac tac agc aag       1009
Gly Gly Ile His Phe Ile Met Leu Ala Ala Tyr Ala Asp Tyr Ser Lys           
            320                 325                 330                   

tca ggg gag cag tac aga tgg ctg gag aag gac ctg gca aag gtg gac       1057
Ser Gly Glu Gln Tyr Arg Trp Leu Glu Lys Asp Leu Ala Lys Val Asp           
        335                 340                 345                       

agg tcg gtg acg ccg tgg ctg gtc gcc ggc tgg cac gcg cca tgg tac       1105
Arg Ser Val Thr Pro Trp Leu Val Ala Gly Trp His Ala Pro Trp Tyr           
    350                 355                 360                           

acc acc tac aag gct cac tac agg gag gtg gag tgc atg aga gtg gcc       1153
Thr Thr Tyr Lys Ala His Tyr Arg Glu Val Glu Cys Met Arg Val Ala           
365                 370                 375                 380           

atg gag gag ctg ctc tac tcc cac ggc ctg gac atc gct ttc acc ggc       1201
Met Glu Glu Leu Leu Tyr Ser His Gly Leu Asp Ile Ala Phe Thr Gly           
                385                 390                 395               

cat gtg cac gcg tac gag cgc tcc aac cgg gtg ttc aac tac acg ctg       1249
His Val His Ala Tyr Glu Arg Ser Asn Arg Val Phe Asn Tyr Thr Leu           
            400                 405                 410                   

gat ccg tgc ggc gcc gtg cac atc tcg gtg ggc gac ggc ggg aac cgc       1297
Asp Pro Cys Gly Ala Val His Ile Ser Val Gly Asp Gly Gly Asn Arg           
        415                 420                 425                       

gag aag atg gcc acc acc cac gcc gac gag ccg ggg cac tgc ccg gac       1345
Glu Lys Met Ala Thr Thr His Ala Asp Glu Pro Gly His Cys Pro Asp           
    430                 435                 440                           

ccg cgg ccc aag ccc aac gcc ttc atc ggc ggc ttc tgc ggc ttt aac       1393
Pro Arg Pro Lys Pro Asn Ala Phe Ile Gly Gly Phe Cys Gly Phe Asn           
445                 450                 455                 460           

ttc acg tcc ggc ccg gcc gcc ggc agg tac tgc tgg gac cgg cag ccg       1441
Phe Thr Ser Gly Pro Ala Ala Gly Arg Tyr Cys Trp Asp Arg Gln Pro           
                465                 470                 475               

gac tac agc gcc tac cgg gag agc agc ttc ggc cac ggc atc ctc gag       1489
Asp Tyr Ser Ala Tyr Arg Glu Ser Ser Phe Gly His Gly Ile Leu Glu           
            480                 485                 490                   

gtg aag aac gag acg cac gct ctg tgg aga tgg cac agg aac cag gac       1537
Val Lys Asn Glu Thr His Ala Leu Trp Arg Trp His Arg Asn Gln Asp           
        495                 500                 505                       

atg tac ggg agc gcc gga gat gag att tac att gtc cgg gag ccg gag       1585
Met Tyr Gly Ser Ala Gly Asp Glu Ile Tyr Ile Val Arg Glu Pro Glu           
    510                 515                 520                           

agg tgc ttg cac aag cac aac tcg acc agg ccg gca cac ggc cga           1630
Arg Cys Leu His Lys His Asn Ser Thr Arg Pro Ala His Gly Arg               
525                 530                 535                               

taaacaccac gcgggaatcg ggagcttaac tgctgtactg ctggagtaga tcgcgcggtg     1690

taatgataac tatatagacg gttcgcccaa gcgtggaaat aaaa                      1734


<210>  28
<211>  539
<212>  PRT
<213>  Secale cereale

<400>  28

Met Trp Leu Gly Ser Leu Arg Leu Leu Leu Leu Leu Ala Ala Ala Val 
1               5                   10                  15      


Thr Ala Ala Ala Glu Pro Ala Ser Thr Leu Met Gly Pro Ser Arg Pro 
            20                  25                  30          


Val Thr Val Ala Leu Arg Glu Asp Arg Gly His Ala Val Asp Leu Pro 
        35                  40                  45              


Asp Thr Asp Pro Arg Val Gln Arg Arg Ala Asn Gly Trp Ala Pro Glu 
    50                  55                  60                  


Gln Ile Ala Val Ala Leu Ser Ala Ala Pro Thr Ser Ala Trp Val Ser 
65                  70                  75                  80  


Trp Ile Thr Gly Glu Phe Gln Met Gly Gly Thr Val Lys Pro Leu Asp 
                85                  90                  95      


Pro Gly Thr Val Gly Ser Val Val Arg Tyr Gly Leu Ala Ala Asp Ser 
            100                 105                 110         


Leu Val Arg Val Ala Thr Gly Asp Ala Leu Val Tyr Ser Gln Leu Tyr 
        115                 120                 125             


Pro Phe Glu Gly Leu Gln Asn Tyr Thr Ser Gly Ile Ile His His Val 
    130                 135                 140                 


Arg Leu Gln Gly Leu Glu Pro Gly Thr Lys Tyr Tyr Tyr Gln Cys Gly 
145                 150                 155                 160 


Asp Pro Ala Leu Pro Gly Thr Met Ser Ala Val His Ala Phe Arg Thr 
                165                 170                 175     


Met Pro Ala Val Gly Pro Arg Ser Tyr Pro Gly Arg Ile Ala Val Val 
            180                 185                 190         


Gly Asp Leu Gly Leu Thr Tyr Asn Thr Thr Ser Thr Val Asp His Met 
        195                 200                 205             


Met Ser Asn Arg Pro Asp Leu Val Val Leu Val Gly Asp Val Ser Tyr 
    210                 215                 220                 


Ala Asn Leu Tyr Leu Thr Asn Gly Thr Gly Ala Asp Cys Tyr Ser Cys 
225                 230                 235                 240 


Ala Phe Gly Lys Ser Thr Pro Ile His Glu Thr Tyr Gln Pro Arg Trp 
                245                 250                 255     


Asp Tyr Trp Gly Arg Tyr Met Glu Ala Val Thr Ser Gly Thr Pro Met 
            260                 265                 270         


Met Val Val Glu Gly Asn His Glu Ile Glu Glu Gln Ile Gly Lys Lys 
        275                 280                 285             


Thr Phe Glu Ala Tyr Arg Ser Arg Phe Ala Phe Pro Ser Ala Glu Asn 
    290                 295                 300                 


Gly Ser Phe Ser Pro Phe Tyr Tyr Ser Phe Asp Ala Gly Gly Ile His 
305                 310                 315                 320 


Phe Ile Met Leu Ala Ala Tyr Ala Asp Tyr Ser Lys Ser Gly Glu Gln 
                325                 330                 335     


Tyr Arg Trp Leu Glu Lys Asp Leu Ala Lys Val Asp Arg Ser Val Thr 
            340                 345                 350         


Pro Trp Leu Val Ala Gly Trp His Ala Pro Trp Tyr Thr Thr Tyr Lys 
        355                 360                 365             


Ala His Tyr Arg Glu Val Glu Cys Met Arg Val Ala Met Glu Glu Leu 
    370                 375                 380                 


Leu Tyr Ser His Gly Leu Asp Ile Ala Phe Thr Gly His Val His Ala 
385                 390                 395                 400 


Tyr Glu Arg Ser Asn Arg Val Phe Asn Tyr Thr Leu Asp Pro Cys Gly 
                405                 410                 415     


Ala Val His Ile Ser Val Gly Asp Gly Gly Asn Arg Glu Lys Met Ala 
            420                 425                 430         


Thr Thr His Ala Asp Glu Pro Gly His Cys Pro Asp Pro Arg Pro Lys 
        435                 440                 445             


Pro Asn Ala Phe Ile Gly Gly Phe Cys Gly Phe Asn Phe Thr Ser Gly 
    450                 455                 460                 


Pro Ala Ala Gly Arg Tyr Cys Trp Asp Arg Gln Pro Asp Tyr Ser Ala 
465                 470                 475                 480 


Tyr Arg Glu Ser Ser Phe Gly His Gly Ile Leu Glu Val Lys Asn Glu 
                485                 490                 495     


Thr His Ala Leu Trp Arg Trp His Arg Asn Gln Asp Met Tyr Gly Ser 
            500                 505                 510         


Ala Gly Asp Glu Ile Tyr Ile Val Arg Glu Pro Glu Arg Cys Leu His 
        515                 520                 525             


Lys His Asn Ser Thr Arg Pro Ala His Gly Arg 
    530                 535                 


<210>  29
<211>  1773
<212>  DNA
<213>  Hordeum vulgare


<220>
<221>  HvPAPhy_a1 phytase cDNA
<222>  (1)..(1773)

<220>
<221>  CDS
<222>  (40)..(1650)
<223>  Phytase coding sequence

<400>  29
agcatcccaa tcctctcaat gccaagcaac aacatcaac atg tgg tgg ggg tcg          54
                                           Met Trp Trp Gly Ser            
                                           1               5              

ctg ctg ctg ctc gcg gcg gcg gtg gcg gtg gct gcc gct gag ccg ccg        102
Leu Leu Leu Leu Ala Ala Ala Val Ala Val Ala Ala Ala Glu Pro Pro           
                10                  15                  20                

tcg acg ctc gct ggc ccg tcg cgg ccg gtg acg gtg acg ccg cgg gaa        150
Ser Thr Leu Ala Gly Pro Ser Arg Pro Val Thr Val Thr Pro Arg Glu           
            25                  30                  35                    

aac agg ggc cac gcg gtg gac ctg ccg gac acg gac ccc cgg gtg cag        198
Asn Arg Gly His Ala Val Asp Leu Pro Asp Thr Asp Pro Arg Val Gln           
        40                  45                  50                        

cgc cgg gcc acg ggc tgg gct ccc gag cag gtc gcc gtc gcg ctc tcc        246
Arg Arg Ala Thr Gly Trp Ala Pro Glu Gln Val Ala Val Ala Leu Ser           
    55                  60                  65                            

gcc gct ccc acc tct gcc tgg gtc tcc tgg atc acc ggg gaa ttc cag        294
Ala Ala Pro Thr Ser Ala Trp Val Ser Trp Ile Thr Gly Glu Phe Gln           
70                  75                  80                  85            

atg ggc ggc acc gtg aag ccg ctg gac ccc cgc acg gtc ggc agc gtc        342
Met Gly Gly Thr Val Lys Pro Leu Asp Pro Arg Thr Val Gly Ser Val           
                90                  95                  100               

gtg cgc tac ggg ctc gcc gcc gac tct ttg gtt cgc gag gcc acc ggc        390
Val Arg Tyr Gly Leu Ala Ala Asp Ser Leu Val Arg Glu Ala Thr Gly           
            105                 110                 115                   

gac gcg ctc gtg tac agc cag ctc tac ccc ttc gag ggc ctc cac aac        438
Asp Ala Leu Val Tyr Ser Gln Leu Tyr Pro Phe Glu Gly Leu His Asn           
        120                 125                 130                       

tac acc tcc ggc atc atc cac cac gtc cgc ctc caa ggg ctt gag cct        486
Tyr Thr Ser Gly Ile Ile His His Val Arg Leu Gln Gly Leu Glu Pro           
    135                 140                 145                           

ggg acc aag tac tac tac cag tgc ggc gac ccg gcc atc ccg ggg gcg        534
Gly Thr Lys Tyr Tyr Tyr Gln Cys Gly Asp Pro Ala Ile Pro Gly Ala           
150                 155                 160                 165           

atg agc gcc gtc cac gcg ttc cgg acg atg ccg gcg gcg ggg ccg cgg        582
Met Ser Ala Val His Ala Phe Arg Thr Met Pro Ala Ala Gly Pro Arg           
                170                 175                 180               

agc tac ccg ggg agg atc gcc gtg gtg gga gac ctc ggg ctc acg tac        630
Ser Tyr Pro Gly Arg Ile Ala Val Val Gly Asp Leu Gly Leu Thr Tyr           
            185                 190                 195                   

aac acc acc tcg acc gtg gac cac atg acg agc aac cgg ccg gac ctg        678
Asn Thr Thr Ser Thr Val Asp His Met Thr Ser Asn Arg Pro Asp Leu           
        200                 205                 210                       

gtc gtc ctc gtc ggc gac gtc agc tac gcc aac atg tac ctc acc aac        726
Val Val Leu Val Gly Asp Val Ser Tyr Ala Asn Met Tyr Leu Thr Asn           
    215                 220                 225                           

ggc acc gga acg gac tgc tac tcc tgc tcc ttc ggc aag tca acg ccc        774
Gly Thr Gly Thr Asp Cys Tyr Ser Cys Ser Phe Gly Lys Ser Thr Pro           
230                 235                 240                 245           

atc cac gaa acc tac cag ccg cgc tgg gac tac tgg gga agg tac atg        822
Ile His Glu Thr Tyr Gln Pro Arg Trp Asp Tyr Trp Gly Arg Tyr Met           
                250                 255                 260               

gag ccg gtg acg tcg agc acg ccg atg atg gtg gtg gaa ggg aac cac        870
Glu Pro Val Thr Ser Ser Thr Pro Met Met Val Val Glu Gly Asn His           
            265                 270                 275                   

gag ata gag gag cag atc ggc aac aag acg ttc gcg gcc tac cgc tcc        918
Glu Ile Glu Glu Gln Ile Gly Asn Lys Thr Phe Ala Ala Tyr Arg Ser           
        280                 285                 290                       

cgg ttc gcg ttc ccg tcg gcg gag agc ggg tcc ttc tcc ccc ttc tac        966
Arg Phe Ala Phe Pro Ser Ala Glu Ser Gly Ser Phe Ser Pro Phe Tyr           
    295                 300                 305                           

tac tcc ttc gac gcc ggc ggg atc cac ttc atc atg ctc ggc gcc tac       1014
Tyr Ser Phe Asp Ala Gly Gly Ile His Phe Ile Met Leu Gly Ala Tyr           
310                 315                 320                 325           

gcc gac tac ggc agg tca ggg gag cag tac aga tgg ctg gag aag gac       1062
Ala Asp Tyr Gly Arg Ser Gly Glu Gln Tyr Arg Trp Leu Glu Lys Asp           
                330                 335                 340               

ctg gcg aag gtg gac agg tcg gtg acc ccc tgg ctg gtg gcc ggc tgg       1110
Leu Ala Lys Val Asp Arg Ser Val Thr Pro Trp Leu Val Ala Gly Trp           
            345                 350                 355                   

cac gcg cca tgg tac acc acg tac aag gct cac tac agg gag gtg gag       1158
His Ala Pro Trp Tyr Thr Thr Tyr Lys Ala His Tyr Arg Glu Val Glu           
        360                 365                 370                       

tgc atg aga gtg gcc atg gag gag ctg ctc tac tcc cac ggc ctc gac       1206
Cys Met Arg Val Ala Met Glu Glu Leu Leu Tyr Ser His Gly Leu Asp           
    375                 380                 385                           

atc gcc ttc acc ggc cat gtg cac gcg tac gag cgc tcc aac cgg gtg       1254
Ile Ala Phe Thr Gly His Val His Ala Tyr Glu Arg Ser Asn Arg Val           
390                 395                 400                 405           

ttc aac tac acg ctg gac ccg tgc ggc gcc gtg tac atc tcg gtg ggc       1302
Phe Asn Tyr Thr Leu Asp Pro Cys Gly Ala Val Tyr Ile Ser Val Gly           
                410                 415                 420               

gac ggc ggg aac cgg gag aag atg gcc acc acc cac gcc gac gag ccg       1350
Asp Gly Gly Asn Arg Glu Lys Met Ala Thr Thr His Ala Asp Glu Pro           
            425                 430                 435                   

ggg cac tgc ccg gac ccg cgg cca aag ccc aac gcc ttc att gcc ggc       1398
Gly His Cys Pro Asp Pro Arg Pro Lys Pro Asn Ala Phe Ile Ala Gly           
        440                 445                 450                       

ttc tgc gcc ttt aac ttc acg tcc ggc ccg gcc gcc ggc agg ttc tgc       1446
Phe Cys Ala Phe Asn Phe Thr Ser Gly Pro Ala Ala Gly Arg Phe Cys           
    455                 460                 465                           

tgg gac cgg cag ccg gac tac agc gcg tac cgg gag agc agc ttc ggc       1494
Trp Asp Arg Gln Pro Asp Tyr Ser Ala Tyr Arg Glu Ser Ser Phe Gly           
470                 475                 480                 485           

cat ggc atc ctc gag gtg aag aac gag acg cac gct ctg tgg aga tgg       1542
His Gly Ile Leu Glu Val Lys Asn Glu Thr His Ala Leu Trp Arg Trp           
                490                 495                 500               

cac agg aac cag gac ctg tac ggg agc gcc gga gat gag att tac att       1590
His Arg Asn Gln Asp Leu Tyr Gly Ser Ala Gly Asp Glu Ile Tyr Ile           
            505                 510                 515                   

gtt cgg gag ccg gag agg tgc ttg cac aag cac aac tcg acc agg ccc       1638
Val Arg Glu Pro Glu Arg Cys Leu His Lys His Asn Ser Thr Arg Pro           
        520                 525                 530                       

gca cac ggt ccg taaaaatggc aactacagac ggttcgccca agccggagat           1690
Ala His Gly Pro                                                           
    535                                                                   

taactgttct accactactg gagtatatcg ccccgtgcaa taatggcaac tatagacggt     1750

tcgcccatgc gtggaaataa aaa                                             1773


<210>  30
<211>  537
<212>  PRT
<213>  Hordeum vulgare

<400>  30

Met Trp Trp Gly Ser Leu Leu Leu Leu Ala Ala Ala Val Ala Val Ala 
1               5                   10                  15      


Ala Ala Glu Pro Pro Ser Thr Leu Ala Gly Pro Ser Arg Pro Val Thr 
            20                  25                  30          


Val Thr Pro Arg Glu Asn Arg Gly His Ala Val Asp Leu Pro Asp Thr 
        35                  40                  45              


Asp Pro Arg Val Gln Arg Arg Ala Thr Gly Trp Ala Pro Glu Gln Val 
    50                  55                  60                  


Ala Val Ala Leu Ser Ala Ala Pro Thr Ser Ala Trp Val Ser Trp Ile 
65                  70                  75                  80  


Thr Gly Glu Phe Gln Met Gly Gly Thr Val Lys Pro Leu Asp Pro Arg 
                85                  90                  95      


Thr Val Gly Ser Val Val Arg Tyr Gly Leu Ala Ala Asp Ser Leu Val 
            100                 105                 110         


Arg Glu Ala Thr Gly Asp Ala Leu Val Tyr Ser Gln Leu Tyr Pro Phe 
        115                 120                 125             


Glu Gly Leu His Asn Tyr Thr Ser Gly Ile Ile His His Val Arg Leu 
    130                 135                 140                 


Gln Gly Leu Glu Pro Gly Thr Lys Tyr Tyr Tyr Gln Cys Gly Asp Pro 
145                 150                 155                 160 


Ala Ile Pro Gly Ala Met Ser Ala Val His Ala Phe Arg Thr Met Pro 
                165                 170                 175     


Ala Ala Gly Pro Arg Ser Tyr Pro Gly Arg Ile Ala Val Val Gly Asp 
            180                 185                 190         


Leu Gly Leu Thr Tyr Asn Thr Thr Ser Thr Val Asp His Met Thr Ser 
        195                 200                 205             


Asn Arg Pro Asp Leu Val Val Leu Val Gly Asp Val Ser Tyr Ala Asn 
    210                 215                 220                 


Met Tyr Leu Thr Asn Gly Thr Gly Thr Asp Cys Tyr Ser Cys Ser Phe 
225                 230                 235                 240 


Gly Lys Ser Thr Pro Ile His Glu Thr Tyr Gln Pro Arg Trp Asp Tyr 
                245                 250                 255     


Trp Gly Arg Tyr Met Glu Pro Val Thr Ser Ser Thr Pro Met Met Val 
            260                 265                 270         


Val Glu Gly Asn His Glu Ile Glu Glu Gln Ile Gly Asn Lys Thr Phe 
        275                 280                 285             


Ala Ala Tyr Arg Ser Arg Phe Ala Phe Pro Ser Ala Glu Ser Gly Ser 
    290                 295                 300                 


Phe Ser Pro Phe Tyr Tyr Ser Phe Asp Ala Gly Gly Ile His Phe Ile 
305                 310                 315                 320 


Met Leu Gly Ala Tyr Ala Asp Tyr Gly Arg Ser Gly Glu Gln Tyr Arg 
                325                 330                 335     


Trp Leu Glu Lys Asp Leu Ala Lys Val Asp Arg Ser Val Thr Pro Trp 
            340                 345                 350         


Leu Val Ala Gly Trp His Ala Pro Trp Tyr Thr Thr Tyr Lys Ala His 
        355                 360                 365             


Tyr Arg Glu Val Glu Cys Met Arg Val Ala Met Glu Glu Leu Leu Tyr 
    370                 375                 380                 


Ser His Gly Leu Asp Ile Ala Phe Thr Gly His Val His Ala Tyr Glu 
385                 390                 395                 400 


Arg Ser Asn Arg Val Phe Asn Tyr Thr Leu Asp Pro Cys Gly Ala Val 
                405                 410                 415     


Tyr Ile Ser Val Gly Asp Gly Gly Asn Arg Glu Lys Met Ala Thr Thr 
            420                 425                 430         


His Ala Asp Glu Pro Gly His Cys Pro Asp Pro Arg Pro Lys Pro Asn 
        435                 440                 445             


Ala Phe Ile Ala Gly Phe Cys Ala Phe Asn Phe Thr Ser Gly Pro Ala 
    450                 455                 460                 


Ala Gly Arg Phe Cys Trp Asp Arg Gln Pro Asp Tyr Ser Ala Tyr Arg 
465                 470                 475                 480 


Glu Ser Ser Phe Gly His Gly Ile Leu Glu Val Lys Asn Glu Thr His 
                485                 490                 495     


Ala Leu Trp Arg Trp His Arg Asn Gln Asp Leu Tyr Gly Ser Ala Gly 
            500                 505                 510         


Asp Glu Ile Tyr Ile Val Arg Glu Pro Glu Arg Cys Leu His Lys His 
        515                 520                 525             


Asn Ser Thr Arg Pro Ala His Gly Pro 
    530                 535         


<210>  31
<211>  19
<212>  DNA
<213>  Triticum sp.


<220>
<221>  Triticum spp PAPhy gene forward primer
<222>  (1)..(19)
<223>  PAP ex3 Fw primer

<400>  31
cttgagcctg ggacgaagt                                                    19


<210>  32
<211>  18
<212>  DNA
<213>  Triticum sp.


<220>
<221>  Triticum spp PAPhy gene reverse primer
<222>  (1)..(18)
<223>  PAP ex3 Rv primer

<400>  32
gagaaggacc cgctctcc                                                     18


<210>  33
<211>  20
<212>  DNA
<213>  Triticum sp.


<220>
<221>  Triticum spp PAPhy promoter forward primer
<222>  (1)..(20)
<223>  TaPAPhy_a1-pro-ex1 Fw

<400>  33
ttatgtgtcc gcgtgaagtg                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Triticum sp.


<220>
<221>  Triticum spp PAPhy promoter reverse primer
<222>  (1)..(20)
<223>  TaPAPhy_a1-pro-ex1 Rv

<400>  34
accaagagtc aatgccatcc                                                   20


<210>  35
<211>  23
<212>  DNA
<213>  Triticum sp.


<220>
<221>  Triticum spp PAPhy promoter forward primer 2
<222>  (1)..(23)
<223>  TaPAPhy_a1 -311 cons Fw

<400>  35
tttggacgag ccatagctgc ata                                               23


<210>  36
<211>  20
<212>  DNA
<213>  Triticum sp.


<220>
<221>  Triticum spp PAPhy promoter reverse primer 2
<222>  (1)..(20)
<223>  TaPAPhy_a1 167 Rv

<400>  36
cgctgcaccc gggggtccgt                                                   20


<210>  37
<211>  22
<212>  DNA
<213>  Triticum sp.


<220>
<221>  Triticum spp PAPhy enhancer forward primer
<222>  (1)..(22)
<223>  AS-PCR enhancer forward primer

<400>  37
caagctacac tttgtagaac ac                                                22


<210>  38
<211>  20
<212>  DNA
<213>  Triticum sp.


<220>
<221>  Triticum spp PAPhy gene reverse primer
<222>  (1)..(20)
<223>  AS-PCR enhancer reverse primer

<400>  38
cgctgcaccc gggggtccgt                                                   20


<210>  39
<211>  25
<212>  DNA
<213>  Triticum sp.


<220>
<221>  HighPhy SNP forward primer
<222>  (1)..(25)

<400>  39
tttcaagcta cactttgtag aacac                                             25


<210>  40
<211>  20
<212>  DNA
<213>  Triticum sp.


<220>
<221>  HighPhy SNP reverse primer
<222>  (1)..(20)

<400>  40
gcactagcca agtttggacg                                                   20


<210>  41
<211>  25
<212>  DNA
<213>  Triticum sp.


<220>
<221>  Wildtype Phy SNP forward primer
<222>  (1)..(25)

<400>  41
tttcaagcta cactttgtag aacat                                             25


<210>  42
<211>  20
<212>  DNA
<213>  Triticum sp.


<220>
<221>  Wildtype Phy SNP reverse primer
<222>  (1)..(20)

<400>  42
gcactagcca agtttggacg                                                   20


<210>  43
<211>  2090
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  Wild type TaPAPhy-a1 promoter
<222>  (1)..(2090)
<223>  Wild type TaPAPhy-a1 promoter and 5' untranslated region

<220>
<221>  wild type cereal enhancer
<222>  (1845)..(1859)

<400>  43
aactcatgct cctaatgtga agcataaaat tatgtgtccg cgtgaagtgc atgtgtactc       60

catgaaaaac ctataaaatg agaaaaccca aaaaaactag aaaaatcttt gcaaaatcca      120

aaaacctaaa aaaaatcccc caaaaaagat tcatgtggag gtgtacgcta tgtggcgaca      180

actgaatgca ccacgtggcg cggtcgtgtg ctcacaccgg ggaaagtgcc accgtggggt      240

accccctaat tatttgctcc aaaagtttgt tgctttgtaa aaaagttata aatcaaacat      300

aatgggcctg atctcattca gctcgaactc cacacacaaa gctagatcga atgctctaac      360

tttgcgaagc tggaatccat cgtctccaag aggcctagtg cgatttttcg aagctagtat      420

ttcatgattt aaaaattcac tatgtttatg aacaactctc gactagagag tattcttagt      480

aatgtgataa gagtaacaca acgatttttt taatataggt ttggtttgca attgtattga      540

tagagcgaga cctaatccta tttgggaagg cctcaggtag ttaggacggt tgtagctcag      600

tcgaggtcag tttgagtcgt aatgcaactc ttcgcacttg acttcaaaat gacttggggc      660

tattgaaagg tccaaaagca acgatgtcgc cgatgagttt attcaaagtg agagtacatg      720

tccatgaggc taagagaagg ggtgccttga caaattatca agactgcacc aaggcgatgg      780

taggatatga gaggtatgat gataagtcta tatccatgag tccataaaag tagtcaaagg      840

tggatccata gtccatggta cattaatcat tttcatccat gtgtgaatgg acctttagct      900

agttgggcct catttagatt tgggctcatt tccttctata cttcattctt gactctcttt      960

ttttttgcga aaaggattag atctcttata aaaattcatc ggaggtacaa agtatctcaa     1020

acataataaa aactacatcg agattccgag accaacgaac gaccaccact gccactagaa     1080

taagctgctg acgcgccacc ggagccgcct tgaccttgtc aatgacagcc gggaagtctt     1140

cacgcacgta cccctaagga ccaacgctct ggagtcgcag tcgtcgccat tgaacgcttg     1200

catagatctg aagcatttga caccaaatct cgccacatga cgagaaaacg ctaaccccac     1260

cgccccaaga agacaacaag aatctatgtc ggagctccgt caactaccca gatgagtgaa     1320

ctcgaggagg atcggagccc ggaagacaaa ctcgaagaag aagccttgcc atccacccga     1380

aggccgcacc tatgaggact aaaaaaacct aacctaattt tttttgataa aagaagggtt     1440

ttccccttcc gattttcatt aaagaaaacc aaaccaaacc taaactacta accggagcga     1500

aggcatcggg attctcgtcc gcgccaccgg ccgccggagc ggtaggcaga gtggaggcaa     1560

atccacggac tcaccggtga agtctagagg ggtctagccg cctaggatca ttatgggagg     1620

taaacaagag tgatttcaat gttgactttg acctttcatt ttttgccttc tccttttatt     1680

tgttgaccat gcaattttgc ttggacttct attaatcttg ttaatggagt tggatatatg     1740

cgttgatcgt tttagacctt attcaccggc ttgaactttt ttggacgagc catagctgca     1800

tattttgttg cttgcgcttt agtttcaagc tacactttgt agaacatgag tcatgcatgg     1860

gacgaaggcg tccaaacttg gctagtgcag ctgcctgcgc gttcacaagg caccaaagcg     1920

caggcggcaa agtttgctcg tttattatct tggcggtcca agatgggcgg caggttccag     1980

acgatggacg aagacccacc gagttccact tccggctcca acctcctctg cccgattcat     2040

ataagtttcc tgccaaaggc attccaattc tgtcaatgcc aagcaacaac                2090


<210>  44
<211>  2060
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  HighPhy mutant TaPAPhy-a1 promoter
<222>  (1)..(2060)
<223>  HighPhy mutant TaPAPhy-a1 promoter and 5' untranslated region

<220>
<221>  Mutant cereal Enhancer
<222>  (1816)..(1829)

<400>  44
ttatgtgtcc gcgtgaagtg catctgtact ccatgaaaaa cctataaaat gagaaaaccc       60

aaaaaaaact agaaaaatct ttgcaaaatc caaaaaccta aaaaaaatcc cccaaaaaag      120

attcatgtgg aggtgtacgc tatgtggcga caactgaatg caccacgtgg cgcggtcgtg      180

tgctcacacc ggggaaagtg ccaccgtggg gtacccccta attatttgct ccaaaagttt      240

gttgctttgt aaaaaaaata taaatcaaac ataatgggcc tgatctcatt cagctcgaac      300

tccacacaca aagctagatc gaatgctcta actttgcgaa gctggaatcc atcgtctcca      360

agaggcctag tgcgattttt cgaagctagt atttcatgat ttaaaaattc actatgttta      420

tgaacaactc tcgactagag agtattctta gtaatgtgat aagagtaaca cagcgatttt      480

tttaatatag gttcggtttg caattgtatt gatagagcga gacctaatcc tatttgggaa      540

ggcctcaggt agttaggacg gttgtagctc agtcgaggtc agtttgagtc gtaatgcaac      600

tcttcgcact tgacttcaaa atgacttggg gctattgaaa ggtccaaaag caacgatgtc      660

gccgatgagt ttattcaaag tgagagtaca tgtccatgag gctaagagaa ggggtgcctt      720

gacaaattat caagactgca ccaaggcgat ggtaggatat gagaggtatg atgataagtc      780

tatatccatg agtccataaa agtagtcaag ggtggatcca tagtccatgg tacattaatc      840

attttcatcc atgtgtgaat ggacctttag ctagttgggc ctcatttaga tttgggctca      900

tttccttcta gacttcattc ttgactcttt tttttgcgaa aaggattaga tctcttataa      960

aaattcatcg gaggtacaaa gtatctcaaa cataataaaa actacatcga gattccgagc     1020

ccaacgaacg accaccactg ccactagaat aagctgctga cgcgccaccg gagccgcctt     1080

gaccttgtca atgacagccg ggaagtcttc acgcacgtac ccctaaggat cgacgctttg     1140

gagtcgcagt cattgccatt gaacacttgc atagatctga agcatttgac accaaatctc     1200

gccacatgac gagaaaccct aaccccaccg ccccaagaag acaacaagaa tctacgtcgg     1260

agctccgtca actacccaga tgagtgaact cgaggaggat cggagcccgg aagacaaact     1320

cgaagaagaa gccttgccat ccacccgaag gccgcactta cgaggactaa aaaaacctaa     1380

cctaaatttt tttttgataa aagaagggtt ttccccttcc gattttcatt aaagaaaacc     1440

aaacctaacc taaactacta accggagcga aggcatcggg attctcgtcc gcgccatcgg     1500

ccgccggagc ggtaggcaga gtggaggcaa atccacggac tcaccggtga agtctggagg     1560

ggtctagccg tctaggatca ttatgggagg taaacaagag tgatttcaat gttgactttg     1620

acctttcatt ttttgccttc tccttttatt tgttgaccat gcaattttgc ttggacttct     1680

attaatcttg ttaatggagt tggatatatg cgttgatcgt tttagacctt attcaccggc     1740

ttgaactttt ttggacgagc catagctgca tattttgttg cttgcgcttt agtttcaagc     1800

tacactttgt agaacacgag tcatgcatgg gacgaaggcg tccaaacttg gctagtgcag     1860

ctgcctgcgc gttcacaagg caccaaagcg caggcggcaa agtttgctcg tttattatct     1920

tggcggtcca agatgggcgg caggttccag acgatggacg aagacccacc gagttccact     1980

tccggctcca acctcctctg cccgattcat ataagtttcc tgccaaaggc atcccaattc     2040

tgtcaatgcc aagcaacaac                                                 2060


<210>  45
<211>  4814
<212>  DNA
<213>  Triticum aestivum


<220>
<221>  promoter
<222>  (1)..(2090)
<223>  Promoter and '5' untranslated region

<220>
<221>  exon
<222>  (2091)..(2342)

<220>
<221>  Intron
<222>  (2343)..(2405)

<220>
<221>  exon
<222>  (2406)..(2597)

<220>
<221>  Intron
<222>  (2598)..(2687)

<220>
<221>  exon
<222>  (2688)..(3827)

<220>
<221>  Intron
<222>  (3828)..(3925)

<220>
<221>  exon
<222>  (3926)..(4090)

<220>
<221>  3'UTR
<222>  (4091)..(4814)

<400>  45
aactcatgct cctaatgtga agcataaaat tatgtgtccg cgtgaagtgc atgtgtactc       60

catgaaaaac ctataaaatg agaaaaccca aaaaaactag aaaaatcttt gcaaaatcca      120

aaaacctaaa aaaaatcccc caaaaaagat tcatgtggag gtgtacgcta tgtggcgaca      180

actgaatgca ccacgtggcg cggtcgtgtg ctcacaccgg ggaaagtgcc accgtggggt      240

accccctaat tatttgctcc aaaagtttgt tgctttgtaa aaaagttata aatcaaacat      300

aatgggcctg atctcattca gctcgaactc cacacacaaa gctagatcga atgctctaac      360

tttgcgaagc tggaatccat cgtctccaag aggcctagtg cgatttttcg aagctagtat      420

ttcatgattt aaaaattcac tatgtttatg aacaactctc gactagagag tattcttagt      480

aatgtgataa gagtaacaca acgatttttt taatataggt ttggtttgca attgtattga      540

tagagcgaga cctaatccta tttgggaagg cctcaggtag ttaggacggt tgtagctcag      600

tcgaggtcag tttgagtcgt aatgcaactc ttcgcacttg acttcaaaat gacttggggc      660

tattgaaagg tccaaaagca acgatgtcgc cgatgagttt attcaaagtg agagtacatg      720

tccatgaggc taagagaagg ggtgccttga caaattatca agactgcacc aaggcgatgg      780

taggatatga gaggtatgat gataagtcta tatccatgag tccataaaag tagtcaaagg      840

tggatccata gtccatggta cattaatcat tttcatccat gtgtgaatgg acctttagct      900

agttgggcct catttagatt tgggctcatt tccttctata cttcattctt gactctcttt      960

ttttttgcga aaaggattag atctcttata aaaattcatc ggaggtacaa agtatctcaa     1020

acataataaa aactacatcg agattccgag accaacgaac gaccaccact gccactagaa     1080

taagctgctg acgcgccacc ggagccgcct tgaccttgtc aatgacagcc gggaagtctt     1140

cacgcacgta cccctaagga ccaacgctct ggagtcgcag tcgtcgccat tgaacgcttg     1200

catagatctg aagcatttga caccaaatct cgccacatga cgagaaaacg ctaaccccac     1260

cgccccaaga agacaacaag aatctatgtc ggagctccgt caactaccca gatgagtgaa     1320

ctcgaggagg atcggagccc ggaagacaaa ctcgaagaag aagccttgcc atccacccga     1380

aggccgcacc tatgaggact aaaaaaacct aacctaattt tttttgataa aagaagggtt     1440

ttccccttcc gattttcatt aaagaaaacc aaaccaaacc taaactacta accggagcga     1500

aggcatcggg attctcgtcc gcgccaccgg ccgccggagc ggtaggcaga gtggaggcaa     1560

atccacggac tcaccggtga agtctagagg ggtctagccg cctaggatca ttatgggagg     1620

taaacaagag tgatttcaat gttgactttg acctttcatt ttttgccttc tccttttatt     1680

tgttgaccat gcaattttgc ttggacttct attaatcttg ttaatggagt tggatatatg     1740

cgttgatcgt tttagacctt attcaccggc ttgaactttt ttggacgagc catagctgca     1800

tattttgttg cttgcgcttt agtttcaagc tacactttgt agaacatgag tcatgcatgg     1860

gacgaaggcg tccaaacttg gctagtgcag ctgcctgcgc gttcacaagg caccaaagcg     1920

caggcggcaa agtttgctcg tttattatct tggcggtcca agatgggcgg caggttccag     1980

acgatggacg aagacccacc gagttccact tccggctcca acctcctctg cccgattcat     2040

ataagtttcc tgccaaaggc attccaattc tgtcaatgcc aagcaacaac atg tgg        2096
                                                       Met Trp            
                                                       1                  

tgg ggg tcg ctg ctg ctg ctg ctg ctg ctc gcg gcc gcg gtg gcg gcg       2144
Trp Gly Ser Leu Leu Leu Leu Leu Leu Leu Ala Ala Ala Val Ala Ala           
        5                   10                  15                        

gct gct gag ccg gcg tcg acg ctc acg ggc ccg tca cgg ccg gtc acg       2192
Ala Ala Glu Pro Ala Ser Thr Leu Thr Gly Pro Ser Arg Pro Val Thr           
    20                  25                  30                            

gtg gcg ctg cgg gaa gac agg ggc cac gcg gtg gac ctg ccg gac acg       2240
Val Ala Leu Arg Glu Asp Arg Gly His Ala Val Asp Leu Pro Asp Thr           
35                  40                  45                  50            

gac ccc cgg gtg cag cgc cgg gcc acg ggc tgg gct ccc gag cag atc       2288
Asp Pro Arg Val Gln Arg Arg Ala Thr Gly Trp Ala Pro Glu Gln Ile           
                55                  60                  65                

gcc gtc gcg ctc tcc gcc gct ccc acc tct gcc tgg gtc tcc tgg atc       2336
Ala Val Ala Leu Ser Ala Ala Pro Thr Ser Ala Trp Val Ser Trp Ile           
            70                  75                  80                    

acc ggt agtaatctgc tcaccggact ctgattcctg ggttggatgg cattgactct        2392
Thr Gly                                                                   
                                                                          

tggttccgca ggg gaa ttc cag atg ggc ggc acc gtc aag ccg ctg gac        2441
               Glu Phe Gln Met Gly Gly Thr Val Lys Pro Leu Asp            
               85                  90                  95                 

ccc ggc acg gtc ggc agc gtc gtg cgc tac ggg ctc gcc gcc gat tct       2489
Pro Gly Thr Val Gly Ser Val Val Arg Tyr Gly Leu Ala Ala Asp Ser           
            100                 105                 110                   

ttg gtt cgc cag gcc agc ggc gac gcg ctc gtg tac agc cag ctc tac       2537
Leu Val Arg Gln Ala Ser Gly Asp Ala Leu Val Tyr Ser Gln Leu Tyr           
        115                 120                 125                       

ccc ttc gag ggt ctc cag aac tac acc tcc ggc atc atc cac cac gtc       2585
Pro Phe Glu Gly Leu Gln Asn Tyr Thr Ser Gly Ile Ile His His Val           
    130                 135                 140                           

cgc ctc caa ggt gaccgccgtc gttcgttgat cccctgttcc acaatcaatt           2637
Arg Leu Gln Gly                                                           
145                                                                       

tttttttttg catatttttt gggagtgcaa tgaagtatct gcatgcaggg ctt gag        2693
                                                       Leu Glu            
                                                           150            

cct gcg acg aag tac tac tac cag tgc ggc gac ccg gcc ctc ccg ggg       2741
Pro Ala Thr Lys Tyr Tyr Tyr Gln Cys Gly Asp Pro Ala Leu Pro Gly           
                155                 160                 165               

gcg atg agc gcc gtc cac gcg ttc cgg acg atg ccg gcg gtg ggg ccg       2789
Ala Met Ser Ala Val His Ala Phe Arg Thr Met Pro Ala Val Gly Pro           
            170                 175                 180                   

cgg agc tac ccg ggg agg atc gcc gtg gtg gga gac ctc ggg ctc acg       2837
Arg Ser Tyr Pro Gly Arg Ile Ala Val Val Gly Asp Leu Gly Leu Thr           
        185                 190                 195                       

tac aac acc acc tcc acc gtg gac cac atg gcg agc aac cgg ccg gac       2885
Tyr Asn Thr Thr Ser Thr Val Asp His Met Ala Ser Asn Arg Pro Asp           
    200                 205                 210                           

ctg gtc ctc ctc gtc ggc gac gtg tgc tac gcc aac atg tac ctc acc       2933
Leu Val Leu Leu Val Gly Asp Val Cys Tyr Ala Asn Met Tyr Leu Thr           
215                 220                 225                 230           

aac ggc acc gga gcg gac tgc tac tcg tgc gcg ttc ggc aag tcg acg       2981
Asn Gly Thr Gly Ala Asp Cys Tyr Ser Cys Ala Phe Gly Lys Ser Thr           
                235                 240                 245               

ccc atc cac gag acg tac cag ccg cgc tgg gac tac tgg gga agg tac       3029
Pro Ile His Glu Thr Tyr Gln Pro Arg Trp Asp Tyr Trp Gly Arg Tyr           
            250                 255                 260                   

atg gag gcg gtg acg tcg ggg acg ccg atg atg gtg gtg gaa ggg aac       3077
Met Glu Ala Val Thr Ser Gly Thr Pro Met Met Val Val Glu Gly Asn           
        265                 270                 275                       

cat gag ata gag gag cag atc ggg aac aag acg ttc gcg gcc tac cgc       3125
His Glu Ile Glu Glu Gln Ile Gly Asn Lys Thr Phe Ala Ala Tyr Arg           
    280                 285                 290                           

tcc cgg ttc gcg ttc ccg tcg acg gag agc ggg tcc ttc tcc ccc ttc       3173
Ser Arg Phe Ala Phe Pro Ser Thr Glu Ser Gly Ser Phe Ser Pro Phe           
295                 300                 305                 310           

tac tac tcg ttc gac gcc ggc ggg atc cat ttc ctc atg ctc ggc gcc       3221
Tyr Tyr Ser Phe Asp Ala Gly Gly Ile His Phe Leu Met Leu Gly Ala           
                315                 320                 325               

tac gcc gac tac ggc agg tca ggg gag cag tac aga tgg ctg gag aag       3269
Tyr Ala Asp Tyr Gly Arg Ser Gly Glu Gln Tyr Arg Trp Leu Glu Lys           
            330                 335                 340                   

gac ctg gcg aag gtg gac agg tcg gtg acg ccg tgg ctg gtc gcc ggc       3317
Asp Leu Ala Lys Val Asp Arg Ser Val Thr Pro Trp Leu Val Ala Gly           
        345                 350                 355                       

tgg cac gcg cca tgg tac acc acc tac aag gct cac tac agg gag gtg       3365
Trp His Ala Pro Trp Tyr Thr Thr Tyr Lys Ala His Tyr Arg Glu Val           
    360                 365                 370                           

gag tgc atg aga gtg gcc atg gag gag ctg ctc tac tcc cac ggc ctc       3413
Glu Cys Met Arg Val Ala Met Glu Glu Leu Leu Tyr Ser His Gly Leu           
375                 380                 385                 390           

gac atc gcc ttc acc ggc cat gta aca cct caa tca cac cct ctg act       3461
Asp Ile Ala Phe Thr Gly His Val Thr Pro Gln Ser His Pro Leu Thr           
                395                 400                 405               

gac acg gat cga cct acc tcc gtt ctc tgg aca ttg gca agc agc cga       3509
Asp Thr Asp Arg Pro Thr Ser Val Leu Trp Thr Leu Ala Ser Ser Arg           
            410                 415                 420                   

gag tga tca ctc gct tgc tgt gtg atg cag gtg cac gcg tat gag cgc       3557
Glu     Ser Leu Ala Cys Cys Val Met Gln Val His Ala Tyr Glu Arg           
            425                 430                 435                   

tcc aac cgg gtg ttc aac tac acg ctg gac ccg tgc ggc gcc gtg cac       3605
Ser Asn Arg Val Phe Asn Tyr Thr Leu Asp Pro Cys Gly Ala Val His           
        440                 445                 450                       

atc tcg gtg ggc gac ggc ggg aac cgc gag aag atg gcc acc acc cac       3653
Ile Ser Val Gly Asp Gly Gly Asn Arg Glu Lys Met Ala Thr Thr His           
    455                 460                 465                           

gcc gac gag ccg ggg cac tgc ccg gac ccg cgg ccc aag ccc aac gcc       3701
Ala Asp Glu Pro Gly His Cys Pro Asp Pro Arg Pro Lys Pro Asn Ala           
470                 475                 480                 485           

ttc atc ggc ggc ttc tgc gcc tcc aac ttc acg tcc ggc ccg gcc gcc       3749
Phe Ile Gly Gly Phe Cys Ala Ser Asn Phe Thr Ser Gly Pro Ala Ala           
                490                 495                 500               

ggc agg ttc tgc tgg gac cgg cag ccg gac tac agc gcc tac cgg gag       3797
Gly Arg Phe Cys Trp Asp Arg Gln Pro Asp Tyr Ser Ala Tyr Arg Glu           
            505                 510                 515                   

agc agc ttc ggc cac ggc atc ctc gag gta cgtacgtacg aggaaaacaa         3847
Ser Ser Phe Gly His Gly Ile Leu Glu Val                                   
        520                 525                                           

gatcgaagag aattctgacc agctagatat atggttcgtt tgaccgatgt gagacgacgc     3907

aattggttca cgcaggtg aag aac gag acg cac gct ctg tgg aga tgg cac       3958
                    Lys Asn Glu Thr His Ala Leu Trp Arg Trp His           
                            530                 535                       

agg aac cag gac cac tac ggg agc gcc gga gat gag att tac att gtc       4006
Arg Asn Gln Asp His Tyr Gly Ser Ala Gly Asp Glu Ile Tyr Ile Val           
    540                 545                 550                           

cgg gag ccg cac agg tgc ttg cac aag cac aac tcg agc agg ccg gca       4054
Arg Glu Pro His Arg Cys Leu His Lys His Asn Ser Ser Arg Pro Ala           
555                 560                 565                 570           

cac ggt cga tca aac acc aca cgg gaa tcg gga ggt taaccgttgt            4100
His Gly Arg Ser Asn Thr Thr Arg Glu Ser Gly Gly                           
                575                 580                                   

accactggag tagatcgcgt ggtgtaatgg caactgtata gacggttcgc ccaagcgtgg     4160

aaataaaaag ttataccaac taaaacatgg attgggcagt gctaggcgct ggccggccgg     4220

ccggcccaaa tttccaacgg tcgtgctagc cgcccgacac cagtcgcact ggccgttgga     4280

tctagcaaaa aaaaaaaaaa accggttcgc gaagctcccc accccaccca caatctcgcg     4340

cagctaaccc cgttgccgcg ctcaccctcc acctgggcgg cgacaccctc cacctatgcc     4400

cgccggcgct cgtccttggt ttcgtgcgtt tctgctccgg tgctcccctc ctgggctgaa     4460

cgcgaggtgg aaaaacacat cgacggccag gatgaaccaa aaacggttaa taacgagggg     4520

cacgatcgtc tttgttgccc cgcgtatagc cgtcgaggag cccagggagg cccgtacgag     4580

cccggttaga tcgtatgccc ggccgtatac gtatttgtat atggcggcgt ttcgtgcacg     4640

cgtggagccg cccgcgcgtg gggcagcacg tgtcacgggt agattccaaa atcgtcccat     4700

catataaatg tggacggcaa ccacctccgg ccgcatcgcc caccattccc ccccgccgca     4760

tcgtctccct ctccccactc ccaattcccc actccgtttc ctctccaaca ttct           4814


<210>  46
<211>  25
<212>  DNA
<213>  Hordeum vulgare


<220>
<221>  HvPAPhy_a SDmut Fw primer
<222>  (1)..(25)
<223>  HvPAPhy_a SDmut Fw primer for mutant enhancer

<400>  46
gtagaacacg agccatgcat gagac                                             25


<210>  47
<211>  25
<212>  DNA
<213>  Hordeum vulgare


<220>
<221>  HvPAPhy_a SDmut Rv primer
<222>  (1)..(25)
<223>  HvPAPhy_a SDmut Rv primer for mutant enhancer

<400>  47
tggctcgtgt tctacaaaat gtagc                                             25


<210>  48
<211>  20
<212>  DNA
<213>  Hordeum vulgare


<220>
<221>  Cis to GUS Fw primer
<222>  (1)..(20)

<400>  48
tcgagtcgac gttccttgac                                                   20


<210>  49
<211>  23
<212>  DNA
<213>  Hordeum vulgare


<220>
<221>  Cis to GUS Rv primer
<222>  (1)..(23)

<400>  49
gttgatgttg ttgcttggca ttg                                               23


<210>  50
<211>  36
<212>  DNA
<213>  Escherichia coli


<220>
<221>  GUS Fw m. overhang primer
<222>  (1)..(36)

<400>  50
agcaacaaca tcaacatgtt acgtcctgta gaaacc                                 36


<210>  51
<211>  38
<212>  DNA
<213>  Escherichia coli


<220>
<221>  GUS Rv m. overhang primer
<222>  (1)..(38)

<400>  51
ggaacgtcga ctcgactatg accatgatta cgaattcc                               38


<210>  52
<211>  8132
<212>  DNA
<213>  Hordeum vulgare


<220>
<221>  pCLEAN-G185-wt-proGUS
<222>  (1)..(8132)

<220>
<221>  Vector backbone, pCLEAN-G185
<222>  (1)..(2599)

<220>
<221>  HvPAPhy_a promoter
<222>  (2600)..(4799)

<220>
<221>  CDS
<222>  (4800)..(6611)
<223>  UidA gene encoding GUS

<220>
<221>  NOS terminator
<222>  (6612)..(6959)

<220>
<221>  Vector backbone, pCLEAN-G185
<222>  (6960)..(8132)

<400>  52
catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt       60

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg      120

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg      180

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag      240

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc      300

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa      360

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg      420

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc      480

taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac      540

cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg      600

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt      660

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt      720

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa      780

atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat      840

caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg      900

tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta      960

tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa     1020

aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa     1080

aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa     1140

atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac     1200

gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac     1260

tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc     1320

tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg     1380

cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt     1440

aacaacattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt     1500

cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata     1560

cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg     1620

ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt     1680

tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg     1740

gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga     1800

caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa     1860

caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc     1920

gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata     1980

gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt     2040

ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa     2100

tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga     2160

gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt     2220

tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt     2280

cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag     2340

cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt     2400

ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct     2460

ggatttccga tccccggaat tagatccgtt taaactacgt aagatcttgg caggatatat     2520

tgtggtgtaa acgttcctgc ggcggtcgag atggatcttg gcaggatata ttgtggtgta     2580

aacgttcctg cggccgcatc ttgggcaaca tatcaggggc agcgccattg ccctgcgact     2640

gacggcggcg gtggaggagc ttggggcaga catgagctga gaacgacgag agagaggagt     2700

ggtggcgggc gagacagagg agcgacatga ttgaagaaga gcagcgggat tgaggattag     2760

ggattcctgc gattttacac ttgacctctc cataaaagat tggcctaatc gaagctgaga     2820

acgtggaggt caacaagtgg tcaaacgagc ctgtacgcac cgcatacgag caacagtgat     2880

cggattttca cgtcacatcg tatatagtga tcgtaaaagc catattctaa agttggatga     2940

ccgtattgtg cttccatgtc aactgcaagg accgtgagtg tatttatctc taaaatataa     3000

atcaaatata atggtgggct tcttcagacc tcattcagct caaaatccgc ccatgaagcc     3060

aatttgaatg ctctaacttt tgcgaagctg gaatctattg tatccaagac tagtgttttc     3120

aaagatagtc ttttcggaat ttcaaaatca ctatgtgtgt tcgtcgtatg aacaactctc     3180

gactagagag tattcttaat aatgcgataa gagtataaca taacaatttt tttgaatgta     3240

ggtttagttg gtctccatgg agcgagacct agtcctattt ggggaggcct ctcgtagttt     3300

ggatggacat agttctgtcg gttcaggttg taatgcaact cctcgcactt gactctaaaa     3360

tgacttaggc tattgaaagg cccaaaaaca atagtattct tcccttgagt tcattcaagt     3420

gagagtatat gtccatgggg ctaagagaag gggtcccttg acaaatgatc aaggttgcgc     3480

aaggtgatga taggtatatt ggtaaatctg tatccacgag ttcatgaagt attcaaaggt     3540

gggggtctat agtccatggt acatcaatca tttccaccca tgtatgaatg ggcctttggc     3600

tagttgggct catttagatt tgggctcagt tacttcttgc cttaaatatt gactttgaca     3660

tttcattttg gtttcccttt ttatttgttg accatgataa tttgcttggc cttttattta     3720

attttttatg gttttgatta ttttttaaac acaatacaga cgaaaacatt catgtacaca     3780

cacatgcatt catctttatg aacatacaca tccacatcat gtccctatca tcttgaaatt     3840

tatgaagtca tagtagacac ctagtcgtcg aggggaattc tcctcggatt gaatgtgtat     3900

cgtcgaaaat tgtgaaataa atgtgagcgc caggacttga atcttgatgg actaggataa     3960

cacagtttct ctaaccatcc aaccgtatgt tggttcgcga tagtttggat tgcttaccac     4020

atgtgtcatg tggttgctag gacttccatt aatctggccg aaccttgtta attgagttgg     4080

atatttcttg accattttag accttattaa gagcatcttc aacaacagtg taaaaaatcc     4140

gcgcccaata aatttttagc gcgccactgt agcactttta aagcgcgggg acggaagcat     4200

cttcaataga cacgcgctaa tgcggcgccc aatccacccc ggtccagcga cccacccaca     4260

acagctcaga gtttgctgcg cgcgcaagcc ggcacaccaa atatgctgtc cgcgatagcg     4320

tttttaagcg cgtgcccaaa attttttagt gcgagcacag ttttgcagcg tctgttggag     4380

ctgttcggcg ccaaaaaacg aatcttttaa cgcgcggtgt agttttgagg cgtctgttgg     4440

agatggtcta accggcttga accgtgttga aaaaaaaacc cggcttgaat ttttgttgac     4500

gagccatagt tgcatattac gtacgttact tgctctttaa tttcaagcta cattttgtag     4560

aacatgagcc atgcatgaga cgtaggcgtc caaactttgg ctagcgcagc tgcatgcacg     4620

tccacaaggc accaaaggcg caggcggcaa ctttgctcgt ttattttctt gcgggtccaa     4680

gatgagttcc agaccatgga cgaattccac ttcgggctcc caatctcctc tgccggattc     4740

ctataagttt cctgccaaga agcatcccaa tcccctcaat gccaagcaac aacatcaac      4799

atg tta cgt cct gta gaa acc cca acc cgt gaa atc aaa aaa ctc gac       4847
Met Leu Arg Pro Val Glu Thr Pro Thr Arg Glu Ile Lys Lys Leu Asp           
1               5                   10                  15                

ggc ctg tgg gca ttc agt ctg gat cgc gaa aac tgt gga att gat cag       4895
Gly Leu Trp Ala Phe Ser Leu Asp Arg Glu Asn Cys Gly Ile Asp Gln           
            20                  25                  30                    

cgt tgg tgg gaa agc gcg tta caa gaa agc cgg gca att gct gtg cca       4943
Arg Trp Trp Glu Ser Ala Leu Gln Glu Ser Arg Ala Ile Ala Val Pro           
        35                  40                  45                        

ggc agt ttt aac gat cag ttc gcc gat gca gat att cgt aat tat gcg       4991
Gly Ser Phe Asn Asp Gln Phe Ala Asp Ala Asp Ile Arg Asn Tyr Ala           
    50                  55                  60                            

ggc aac gtc tgg tat cag cgc gaa gtc ttt ata ccg aaa ggt tgg gca       5039
Gly Asn Val Trp Tyr Gln Arg Glu Val Phe Ile Pro Lys Gly Trp Ala           
65                  70                  75                  80            

ggc cag cgt atc gtg ctg cgt ttc gat gcg gtc act cat tac ggc aaa       5087
Gly Gln Arg Ile Val Leu Arg Phe Asp Ala Val Thr His Tyr Gly Lys           
                85                  90                  95                

gtg tgg gtc aat aat cag gaa gtg atg gag cat cag ggc ggc tat acg       5135
Val Trp Val Asn Asn Gln Glu Val Met Glu His Gln Gly Gly Tyr Thr           
            100                 105                 110                   

cca ttt gaa gcc gat gtc acg ccg tat gtt att gcc ggg aaa agt gta       5183
Pro Phe Glu Ala Asp Val Thr Pro Tyr Val Ile Ala Gly Lys Ser Val           
        115                 120                 125                       

cgt atc acc gtt tgt gtg aac aac gaa ctg aac tgg cag act atc ccg       5231
Arg Ile Thr Val Cys Val Asn Asn Glu Leu Asn Trp Gln Thr Ile Pro           
    130                 135                 140                           

ccg gga atg gtg att acc gac gaa aac ggc aag aaa aag cag tct tac       5279
Pro Gly Met Val Ile Thr Asp Glu Asn Gly Lys Lys Lys Gln Ser Tyr           
145                 150                 155                 160           

ttc cat gat ttc ttt aac tat gcc gga atc cat cgc agc gta atg ctc       5327
Phe His Asp Phe Phe Asn Tyr Ala Gly Ile His Arg Ser Val Met Leu           
                165                 170                 175               

tac acc acg ccg aac acc tgg gtg gac gat atc acc gtg gtg acg cat       5375
Tyr Thr Thr Pro Asn Thr Trp Val Asp Asp Ile Thr Val Val Thr His           
            180                 185                 190                   

gtc gcg caa gac tgt aac cac gcg tct gtt gac tgg cag gtg gtg gcc       5423
Val Ala Gln Asp Cys Asn His Ala Ser Val Asp Trp Gln Val Val Ala           
        195                 200                 205                       

aat ggt gat gtc agc gtt gaa ctg cgt gat gcg gat caa cag gtg gtt       5471
Asn Gly Asp Val Ser Val Glu Leu Arg Asp Ala Asp Gln Gln Val Val           
    210                 215                 220                           

gca act gga caa ggc act agc ggg act ttg caa gtg gtg aat ccg cac       5519
Ala Thr Gly Gln Gly Thr Ser Gly Thr Leu Gln Val Val Asn Pro His           
225                 230                 235                 240           

ctc tgg caa ccg ggt gaa ggt tat ctc tat gaa ctg tgc gtc aca gcc       5567
Leu Trp Gln Pro Gly Glu Gly Tyr Leu Tyr Glu Leu Cys Val Thr Ala           
                245                 250                 255               

aaa agc cag aca gag tgt gat atc tac ccg ctt cgc gtc ggc atc cgg       5615
Lys Ser Gln Thr Glu Cys Asp Ile Tyr Pro Leu Arg Val Gly Ile Arg           
            260                 265                 270                   

tca gtg gca gtg aag ggc gaa cag ttc ctg att aac cac aaa ccg ttc       5663
Ser Val Ala Val Lys Gly Glu Gln Phe Leu Ile Asn His Lys Pro Phe           
        275                 280                 285                       

tac ttt act ggc ttt ggt cgt cat gaa gat gcg gac ttg cgt ggc aaa       5711
Tyr Phe Thr Gly Phe Gly Arg His Glu Asp Ala Asp Leu Arg Gly Lys           
    290                 295                 300                           

gga ttc gat aac gtg ctg atg gtg cac gac cac gca tta atg gac tgg       5759
Gly Phe Asp Asn Val Leu Met Val His Asp His Ala Leu Met Asp Trp           
305                 310                 315                 320           

att ggg gcc aac tcc tac cgt acc tcg cat tac cct tac gct gaa gag       5807
Ile Gly Ala Asn Ser Tyr Arg Thr Ser His Tyr Pro Tyr Ala Glu Glu           
                325                 330                 335               

atg ctc gac tgg gca gat gaa cat ggc atc gtg gtg att gat gaa act       5855
Met Leu Asp Trp Ala Asp Glu His Gly Ile Val Val Ile Asp Glu Thr           
            340                 345                 350                   

gct gct gtc ggc ttt aac ctc tct tta ggc att ggt ttc gaa gcg ggc       5903
Ala Ala Val Gly Phe Asn Leu Ser Leu Gly Ile Gly Phe Glu Ala Gly           
        355                 360                 365                       

aac aag ccg aaa gaa ctg tac agc gaa gag gca gtc aac ggg gaa act       5951
Asn Lys Pro Lys Glu Leu Tyr Ser Glu Glu Ala Val Asn Gly Glu Thr           
    370                 375                 380                           

cag caa gcg cac tta cag gcg att aaa gag ctg ata gcg cgt gac aaa       5999
Gln Gln Ala His Leu Gln Ala Ile Lys Glu Leu Ile Ala Arg Asp Lys           
385                 390                 395                 400           

aac cac cca agc gtg gtg atg tgg agt att gcc aac gaa ccg gat acc       6047
Asn His Pro Ser Val Val Met Trp Ser Ile Ala Asn Glu Pro Asp Thr           
                405                 410                 415               

cgt ccg caa ggt gca cgg gaa tat ttc gcg cca ctg gcg gaa gca acg       6095
Arg Pro Gln Gly Ala Arg Glu Tyr Phe Ala Pro Leu Ala Glu Ala Thr           
            420                 425                 430                   

cgt aaa ctc gac ccg acg cgt ccg atc acc tgc gtc aat gta atg ttc       6143
Arg Lys Leu Asp Pro Thr Arg Pro Ile Thr Cys Val Asn Val Met Phe           
        435                 440                 445                       

tgc gac gct cac acc gat acc atc agc gat ctc ttt gat gtg ctg tgc       6191
Cys Asp Ala His Thr Asp Thr Ile Ser Asp Leu Phe Asp Val Leu Cys           
    450                 455                 460                           

ctg aac cgt tat tac gga tgg tat gtc caa agc ggc gat ttg gaa acg       6239
Leu Asn Arg Tyr Tyr Gly Trp Tyr Val Gln Ser Gly Asp Leu Glu Thr           
465                 470                 475                 480           

gca gag aag gta ctg gaa aaa gaa ctt ctg gcc tgg cag gag aaa ctg       6287
Ala Glu Lys Val Leu Glu Lys Glu Leu Leu Ala Trp Gln Glu Lys Leu           
                485                 490                 495               

cat cag ccg att atc atc acc gaa tac ggc gtg gat acg tta gcc ggg       6335
His Gln Pro Ile Ile Ile Thr Glu Tyr Gly Val Asp Thr Leu Ala Gly           
            500                 505                 510                   

ctg cac tca atg tac acc gac atg tgg agt gaa gag tat cag tgt gca       6383
Leu His Ser Met Tyr Thr Asp Met Trp Ser Glu Glu Tyr Gln Cys Ala           
        515                 520                 525                       

tgg ctg gat atg tat cac cgc gtc ttt gat cgc gtc agc gcc gtc gtc       6431
Trp Leu Asp Met Tyr His Arg Val Phe Asp Arg Val Ser Ala Val Val           
    530                 535                 540                           

ggt gaa cag gta tgg aat ttc gcc gat ttt gcg acc tcg caa ggc ata       6479
Gly Glu Gln Val Trp Asn Phe Ala Asp Phe Ala Thr Ser Gln Gly Ile           
545                 550                 555                 560           

ttg cgc gtt ggc ggt aac aag aaa ggg atc ttc act cgc gac cgc aaa       6527
Leu Arg Val Gly Gly Asn Lys Lys Gly Ile Phe Thr Arg Asp Arg Lys           
                565                 570                 575               

ccg aag tcg gcg gct ttt ctg ctg caa aaa cgc tgg act ggc atg aac       6575
Pro Lys Ser Ala Ala Phe Leu Leu Gln Lys Arg Trp Thr Gly Met Asn           
            580                 585                 590                   

ttc ggt gaa aaa ccg cag cag gga ggc aaa caa tga atcaacaact            6621
Phe Gly Glu Lys Pro Gln Gln Gly Gly Lys Gln                               
        595                 600                                           

ctcctggcgc accatcgtcg gctacagcct cgggaattgc taccgagctc gaatttcccc     6681

gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg     6741

atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc     6801

atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac     6861

gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct     6921

atgttactag atcgggaatt cgtaatcatg gtcatagtcg agtcgacgtt ccttgacagg     6981

atatattggc gggtaaacta agtcgctgta tgtgtttgtt tgagatcctc tagggcatgc     7041

aggctcgcgg cggacgcacg acgccggggc gagaccatag gcgatctcct aaatcaatag     7101

tagctgtaac ctcgaagcgt ttcacttgta acaacgattg agaatttttg tcataaaatt     7161

gaaatacttg gttcgcattt ttgtcatccg cggtcagccg caattctgac gaactgccca     7221

tttagctgga gatgattgta catccttcac gtgaaaattt ctcaagcgct gtgaacaagg     7281

gttcagattt tagattgaaa ggtgagccgt tgaaacacgt tcttcttgtc gatgacgacg     7341

tcgctatgcg gcatcttatt attgaatacc ttacgatcca cgccttcaaa gtgaccgcgg     7401

tagccgacag cacccagttc acaagagtac tctcttccgc gacggtcgat gtcgtggttg     7461

ttgatctaaa tttaggtcgt gaagatgggc tcgagatcgt tcgtaatctg gcggcaaagt     7521

ctgatattcc aatcataatt atcagtggcg accgccttga ggagacggat aaagttgttg     7581

cactcgagct aggagcaagt gattttatcg ctaagccgtt cagtatcaga gagtttctag     7641

cacgcattcg ggttgccttg cgcgtgcgcc ccaacgttgt ccgctccaaa gaccgacggt     7701

ctttttgttt tactgactgg acacttaatc tcaggcaacg tcgcttgatg tccgaagctg     7761

gcggtgaggt gaaacttacg gcaggtgagt tcaatcttct cctcgcgttt ttagagaaac     7821

cccgcgacgt tctatcgcgc gagcaacttc tcattgccag tcgagtacgc gacgaggagg     7881

tttatgacag gagtatagat gttctcattt tgaggctgcg ccgcaaactt gaggcggatc     7941

cgtcaagccc tcaactgata aaaacagcaa gaggtgccgg ttatttcttt gacgcggacg     8001

tgcaggtttc gcacgggggg acgatggcag cctgagccaa ttgcatttgc ctcttaatta     8061

tctggctcaa agggtgactg aggagtaagc gatgtgccca tcacactgcg catgcaagct     8121

gatctggatc t                                                          8132


<210>  53
<211>  603
<212>  PRT
<213>  Hordeum vulgare

<400>  53

Met Leu Arg Pro Val Glu Thr Pro Thr Arg Glu Ile Lys Lys Leu Asp 
1               5                   10                  15      


Gly Leu Trp Ala Phe Ser Leu Asp Arg Glu Asn Cys Gly Ile Asp Gln 
            20                  25                  30          


Arg Trp Trp Glu Ser Ala Leu Gln Glu Ser Arg Ala Ile Ala Val Pro 
        35                  40                  45              


Gly Ser Phe Asn Asp Gln Phe Ala Asp Ala Asp Ile Arg Asn Tyr Ala 
    50                  55                  60                  


Gly Asn Val Trp Tyr Gln Arg Glu Val Phe Ile Pro Lys Gly Trp Ala 
65                  70                  75                  80  


Gly Gln Arg Ile Val Leu Arg Phe Asp Ala Val Thr His Tyr Gly Lys 
                85                  90                  95      


Val Trp Val Asn Asn Gln Glu Val Met Glu His Gln Gly Gly Tyr Thr 
            100                 105                 110         


Pro Phe Glu Ala Asp Val Thr Pro Tyr Val Ile Ala Gly Lys Ser Val 
        115                 120                 125             


Arg Ile Thr Val Cys Val Asn Asn Glu Leu Asn Trp Gln Thr Ile Pro 
    130                 135                 140                 


Pro Gly Met Val Ile Thr Asp Glu Asn Gly Lys Lys Lys Gln Ser Tyr 
145                 150                 155                 160 


Phe His Asp Phe Phe Asn Tyr Ala Gly Ile His Arg Ser Val Met Leu 
                165                 170                 175     


Tyr Thr Thr Pro Asn Thr Trp Val Asp Asp Ile Thr Val Val Thr His 
            180                 185                 190         


Val Ala Gln Asp Cys Asn His Ala Ser Val Asp Trp Gln Val Val Ala 
        195                 200                 205             


Asn Gly Asp Val Ser Val Glu Leu Arg Asp Ala Asp Gln Gln Val Val 
    210                 215                 220                 


Ala Thr Gly Gln Gly Thr Ser Gly Thr Leu Gln Val Val Asn Pro His 
225                 230                 235                 240 


Leu Trp Gln Pro Gly Glu Gly Tyr Leu Tyr Glu Leu Cys Val Thr Ala 
                245                 250                 255     


Lys Ser Gln Thr Glu Cys Asp Ile Tyr Pro Leu Arg Val Gly Ile Arg 
            260                 265                 270         


Ser Val Ala Val Lys Gly Glu Gln Phe Leu Ile Asn His Lys Pro Phe 
        275                 280                 285             


Tyr Phe Thr Gly Phe Gly Arg His Glu Asp Ala Asp Leu Arg Gly Lys 
    290                 295                 300                 


Gly Phe Asp Asn Val Leu Met Val His Asp His Ala Leu Met Asp Trp 
305                 310                 315                 320 


Ile Gly Ala Asn Ser Tyr Arg Thr Ser His Tyr Pro Tyr Ala Glu Glu 
                325                 330                 335     


Met Leu Asp Trp Ala Asp Glu His Gly Ile Val Val Ile Asp Glu Thr 
            340                 345                 350         


Ala Ala Val Gly Phe Asn Leu Ser Leu Gly Ile Gly Phe Glu Ala Gly 
        355                 360                 365             


Asn Lys Pro Lys Glu Leu Tyr Ser Glu Glu Ala Val Asn Gly Glu Thr 
    370                 375                 380                 


Gln Gln Ala His Leu Gln Ala Ile Lys Glu Leu Ile Ala Arg Asp Lys 
385                 390                 395                 400 


Asn His Pro Ser Val Val Met Trp Ser Ile Ala Asn Glu Pro Asp Thr 
                405                 410                 415     


Arg Pro Gln Gly Ala Arg Glu Tyr Phe Ala Pro Leu Ala Glu Ala Thr 
            420                 425                 430         


Arg Lys Leu Asp Pro Thr Arg Pro Ile Thr Cys Val Asn Val Met Phe 
        435                 440                 445             


Cys Asp Ala His Thr Asp Thr Ile Ser Asp Leu Phe Asp Val Leu Cys 
    450                 455                 460                 


Leu Asn Arg Tyr Tyr Gly Trp Tyr Val Gln Ser Gly Asp Leu Glu Thr 
465                 470                 475                 480 


Ala Glu Lys Val Leu Glu Lys Glu Leu Leu Ala Trp Gln Glu Lys Leu 
                485                 490                 495     


His Gln Pro Ile Ile Ile Thr Glu Tyr Gly Val Asp Thr Leu Ala Gly 
            500                 505                 510         


Leu His Ser Met Tyr Thr Asp Met Trp Ser Glu Glu Tyr Gln Cys Ala 
        515                 520                 525             


Trp Leu Asp Met Tyr His Arg Val Phe Asp Arg Val Ser Ala Val Val 
    530                 535                 540                 


Gly Glu Gln Val Trp Asn Phe Ala Asp Phe Ala Thr Ser Gln Gly Ile 
545                 550                 555                 560 


Leu Arg Val Gly Gly Asn Lys Lys Gly Ile Phe Thr Arg Asp Arg Lys 
                565                 570                 575     


Pro Lys Ser Ala Ala Phe Leu Leu Gln Lys Arg Trp Thr Gly Met Asn 
            580                 585                 590         


Phe Gly Glu Lys Pro Gln Gln Gly Gly Lys Gln 
        595                 600             


<210>  54
<211>  8132
<212>  DNA
<213>  Hordeum vulgare


<220>
<221>  pCLEAN-G185-HP-proGUS
<222>  (1)..(8132)

<220>
<221>  Vector backbone, pCLEAN-G185
<222>  (1)..(2599)

<220>
<221>  HighPhy HvPAPhy_a promoter
<222>  (2600)..(4799)

<220>
<221>  HighPhy enhancer mutation
<222>  (4565)..(4565)

<220>
<221>  CDS
<222>  (4800)..(6611)
<223>  UidA gene (encoding GUS)

<220>
<221>  NOS terminator
<222>  (6612)..(6959)

<220>
<221>  Vector backbone, pCLEAN-G185
<222>  (6960)..(8132)

<400>  54
catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt       60

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg      120

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg      180

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag      240

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc      300

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa      360

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg      420

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc      480

taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac      540

cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg      600

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt      660

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt      720

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa      780

atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat      840

caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg      900

tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta      960

tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa     1020

aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa     1080

aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa     1140

atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac     1200

gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac     1260

tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc     1320

tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg     1380

cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt     1440

aacaacattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt     1500

cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata     1560

cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg     1620

ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt     1680

tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg     1740

gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga     1800

caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa     1860

caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc     1920

gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata     1980

gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt     2040

ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa     2100

tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga     2160

gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt     2220

tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt     2280

cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag     2340

cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt     2400

ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct     2460

ggatttccga tccccggaat tagatccgtt taaactacgt aagatcttgg caggatatat     2520

tgtggtgtaa acgttcctgc ggcggtcgag atggatcttg gcaggatata ttgtggtgta     2580

aacgttcctg cggccgcatc ttgggcaaca tatcaggggc agcgccattg ccctgcgact     2640

gacggcggcg gtggaggagc ttggggcaga catgagctga gaacgacgag agagaggagt     2700

ggtggcgggc gagacagagg agcgacatga ttgaagaaga gcagcgggat tgaggattag     2760

ggattcctgc gattttacac ttgacctctc cataaaagat tggcctaatc gaagctgaga     2820

acgtggaggt caacaagtgg tcaaacgagc ctgtacgcac cgcatacgag caacagtgat     2880

cggattttca cgtcacatcg tatatagtga tcgtaaaagc catattctaa agttggatga     2940

ccgtattgtg cttccatgtc aactgcaagg accgtgagtg tatttatctc taaaatataa     3000

atcaaatata atggtgggct tcttcagacc tcattcagct caaaatccgc ccatgaagcc     3060

aatttgaatg ctctaacttt tgcgaagctg gaatctattg tatccaagac tagtgttttc     3120

aaagatagtc ttttcggaat ttcaaaatca ctatgtgtgt tcgtcgtatg aacaactctc     3180

gactagagag tattcttaat aatgcgataa gagtataaca taacaatttt tttgaatgta     3240

ggtttagttg gtctccatgg agcgagacct agtcctattt ggggaggcct ctcgtagttt     3300

ggatggacat agttctgtcg gttcaggttg taatgcaact cctcgcactt gactctaaaa     3360

tgacttaggc tattgaaagg cccaaaaaca atagtattct tcccttgagt tcattcaagt     3420

gagagtatat gtccatgggg ctaagagaag gggtcccttg acaaatgatc aaggttgcgc     3480

aaggtgatga taggtatatt ggtaaatctg tatccacgag ttcatgaagt attcaaaggt     3540

gggggtctat agtccatggt acatcaatca tttccaccca tgtatgaatg ggcctttggc     3600

tagttgggct catttagatt tgggctcagt tacttcttgc cttaaatatt gactttgaca     3660

tttcattttg gtttcccttt ttatttgttg accatgataa tttgcttggc cttttattta     3720

attttttatg gttttgatta ttttttaaac acaatacaga cgaaaacatt catgtacaca     3780

cacatgcatt catctttatg aacatacaca tccacatcat gtccctatca tcttgaaatt     3840

tatgaagtca tagtagacac ctagtcgtcg aggggaattc tcctcggatt gaatgtgtat     3900

cgtcgaaaat tgtgaaataa atgtgagcgc caggacttga atcttgatgg actaggataa     3960

cacagtttct ctaaccatcc aaccgtatgt tggttcgcga tagtttggat tgcttaccac     4020

atgtgtcatg tggttgctag gacttccatt aatctggccg aaccttgtta attgagttgg     4080

atatttcttg accattttag accttattaa gagcatcttc aacaacagtg taaaaaatcc     4140

gcgcccaata aatttttagc gcgccactgt agcactttta aagcgcgggg acggaagcat     4200

cttcaataga cacgcgctaa tgcggcgccc aatccacccc ggtccagcga cccacccaca     4260

acagctcaga gtttgctgcg cgcgcaagcc ggcacaccaa atatgctgtc cgcgatagcg     4320

tttttaagcg cgtgcccaaa attttttagt gcgagcacag ttttgcagcg tctgttggag     4380

ctgttcggcg ccaaaaaacg aatcttttaa cgcgcggtgt agttttgagg cgtctgttgg     4440

agatggtcta accggcttga accgtgttga aaaaaaaacc cggcttgaat ttttgttgac     4500

gagccatagt tgcatattac gtacgttact tgctctttaa tttcaagcta cattttgtag     4560

aacacgagcc atgcatgaga cgtaggcgtc caaactttgg ctagcgcagc tgcatgcacg     4620

tccacaaggc accaaaggcg caggcggcaa ctttgctcgt ttattttctt gcgggtccaa     4680

gatgagttcc agaccatgga cgaattccac ttcgggctcc caatctcctc tgccggattc     4740

ctataagttt cctgccaaga agcatcccaa tcccctcaat gccaagcaac aacatcaac      4799

atg tta cgt cct gta gaa acc cca acc cgt gaa atc aaa aaa ctc gac       4847
Met Leu Arg Pro Val Glu Thr Pro Thr Arg Glu Ile Lys Lys Leu Asp           
1               5                   10                  15                

ggc ctg tgg gca ttc agt ctg gat cgc gaa aac tgt gga att gat cag       4895
Gly Leu Trp Ala Phe Ser Leu Asp Arg Glu Asn Cys Gly Ile Asp Gln           
            20                  25                  30                    

cgt tgg tgg gaa agc gcg tta caa gaa agc cgg gca att gct gtg cca       4943
Arg Trp Trp Glu Ser Ala Leu Gln Glu Ser Arg Ala Ile Ala Val Pro           
        35                  40                  45                        

ggc agt ttt aac gat cag ttc gcc gat gca gat att cgt aat tat gcg       4991
Gly Ser Phe Asn Asp Gln Phe Ala Asp Ala Asp Ile Arg Asn Tyr Ala           
    50                  55                  60                            

ggc aac gtc tgg tat cag cgc gaa gtc ttt ata ccg aaa ggt tgg gca       5039
Gly Asn Val Trp Tyr Gln Arg Glu Val Phe Ile Pro Lys Gly Trp Ala           
65                  70                  75                  80            

ggc cag cgt atc gtg ctg cgt ttc gat gcg gtc act cat tac ggc aaa       5087
Gly Gln Arg Ile Val Leu Arg Phe Asp Ala Val Thr His Tyr Gly Lys           
                85                  90                  95                

gtg tgg gtc aat aat cag gaa gtg atg gag cat cag ggc ggc tat acg       5135
Val Trp Val Asn Asn Gln Glu Val Met Glu His Gln Gly Gly Tyr Thr           
            100                 105                 110                   

cca ttt gaa gcc gat gtc acg ccg tat gtt att gcc ggg aaa agt gta       5183
Pro Phe Glu Ala Asp Val Thr Pro Tyr Val Ile Ala Gly Lys Ser Val           
        115                 120                 125                       

cgt atc acc gtt tgt gtg aac aac gaa ctg aac tgg cag act atc ccg       5231
Arg Ile Thr Val Cys Val Asn Asn Glu Leu Asn Trp Gln Thr Ile Pro           
    130                 135                 140                           

ccg gga atg gtg att acc gac gaa aac ggc aag aaa aag cag tct tac       5279
Pro Gly Met Val Ile Thr Asp Glu Asn Gly Lys Lys Lys Gln Ser Tyr           
145                 150                 155                 160           

ttc cat gat ttc ttt aac tat gcc gga atc cat cgc agc gta atg ctc       5327
Phe His Asp Phe Phe Asn Tyr Ala Gly Ile His Arg Ser Val Met Leu           
                165                 170                 175               

tac acc acg ccg aac acc tgg gtg gac gat atc acc gtg gtg acg cat       5375
Tyr Thr Thr Pro Asn Thr Trp Val Asp Asp Ile Thr Val Val Thr His           
            180                 185                 190                   

gtc gcg caa gac tgt aac cac gcg tct gtt gac tgg cag gtg gtg gcc       5423
Val Ala Gln Asp Cys Asn His Ala Ser Val Asp Trp Gln Val Val Ala           
        195                 200                 205                       

aat ggt gat gtc agc gtt gaa ctg cgt gat gcg gat caa cag gtg gtt       5471
Asn Gly Asp Val Ser Val Glu Leu Arg Asp Ala Asp Gln Gln Val Val           
    210                 215                 220                           

gca act gga caa ggc act agc ggg act ttg caa gtg gtg aat ccg cac       5519
Ala Thr Gly Gln Gly Thr Ser Gly Thr Leu Gln Val Val Asn Pro His           
225                 230                 235                 240           

ctc tgg caa ccg ggt gaa ggt tat ctc tat gaa ctg tgc gtc aca gcc       5567
Leu Trp Gln Pro Gly Glu Gly Tyr Leu Tyr Glu Leu Cys Val Thr Ala           
                245                 250                 255               

aaa agc cag aca gag tgt gat atc tac ccg ctt cgc gtc ggc atc cgg       5615
Lys Ser Gln Thr Glu Cys Asp Ile Tyr Pro Leu Arg Val Gly Ile Arg           
            260                 265                 270                   

tca gtg gca gtg aag ggc gaa cag ttc ctg att aac cac aaa ccg ttc       5663
Ser Val Ala Val Lys Gly Glu Gln Phe Leu Ile Asn His Lys Pro Phe           
        275                 280                 285                       

tac ttt act ggc ttt ggt cgt cat gaa gat gcg gac ttg cgt ggc aaa       5711
Tyr Phe Thr Gly Phe Gly Arg His Glu Asp Ala Asp Leu Arg Gly Lys           
    290                 295                 300                           

gga ttc gat aac gtg ctg atg gtg cac gac cac gca tta atg gac tgg       5759
Gly Phe Asp Asn Val Leu Met Val His Asp His Ala Leu Met Asp Trp           
305                 310                 315                 320           

att ggg gcc aac tcc tac cgt acc tcg cat tac cct tac gct gaa gag       5807
Ile Gly Ala Asn Ser Tyr Arg Thr Ser His Tyr Pro Tyr Ala Glu Glu           
                325                 330                 335               

atg ctc gac tgg gca gat gaa cat ggc atc gtg gtg att gat gaa act       5855
Met Leu Asp Trp Ala Asp Glu His Gly Ile Val Val Ile Asp Glu Thr           
            340                 345                 350                   

gct gct gtc ggc ttt aac ctc tct tta ggc att ggt ttc gaa gcg ggc       5903
Ala Ala Val Gly Phe Asn Leu Ser Leu Gly Ile Gly Phe Glu Ala Gly           
        355                 360                 365                       

aac aag ccg aaa gaa ctg tac agc gaa gag gca gtc aac ggg gaa act       5951
Asn Lys Pro Lys Glu Leu Tyr Ser Glu Glu Ala Val Asn Gly Glu Thr           
    370                 375                 380                           

cag caa gcg cac tta cag gcg att aaa gag ctg ata gcg cgt gac aaa       5999
Gln Gln Ala His Leu Gln Ala Ile Lys Glu Leu Ile Ala Arg Asp Lys           
385                 390                 395                 400           

aac cac cca agc gtg gtg atg tgg agt att gcc aac gaa ccg gat acc       6047
Asn His Pro Ser Val Val Met Trp Ser Ile Ala Asn Glu Pro Asp Thr           
                405                 410                 415               

cgt ccg caa ggt gca cgg gaa tat ttc gcg cca ctg gcg gaa gca acg       6095
Arg Pro Gln Gly Ala Arg Glu Tyr Phe Ala Pro Leu Ala Glu Ala Thr           
            420                 425                 430                   

cgt aaa ctc gac ccg acg cgt ccg atc acc tgc gtc aat gta atg ttc       6143
Arg Lys Leu Asp Pro Thr Arg Pro Ile Thr Cys Val Asn Val Met Phe           
        435                 440                 445                       

tgc gac gct cac acc gat acc atc agc gat ctc ttt gat gtg ctg tgc       6191
Cys Asp Ala His Thr Asp Thr Ile Ser Asp Leu Phe Asp Val Leu Cys           
    450                 455                 460                           

ctg aac cgt tat tac gga tgg tat gtc caa agc ggc gat ttg gaa acg       6239
Leu Asn Arg Tyr Tyr Gly Trp Tyr Val Gln Ser Gly Asp Leu Glu Thr           
465                 470                 475                 480           

gca gag aag gta ctg gaa aaa gaa ctt ctg gcc tgg cag gag aaa ctg       6287
Ala Glu Lys Val Leu Glu Lys Glu Leu Leu Ala Trp Gln Glu Lys Leu           
                485                 490                 495               

cat cag ccg att atc atc acc gaa tac ggc gtg gat acg tta gcc ggg       6335
His Gln Pro Ile Ile Ile Thr Glu Tyr Gly Val Asp Thr Leu Ala Gly           
            500                 505                 510                   

ctg cac tca atg tac acc gac atg tgg agt gaa gag tat cag tgt gca       6383
Leu His Ser Met Tyr Thr Asp Met Trp Ser Glu Glu Tyr Gln Cys Ala           
        515                 520                 525                       

tgg ctg gat atg tat cac cgc gtc ttt gat cgc gtc agc gcc gtc gtc       6431
Trp Leu Asp Met Tyr His Arg Val Phe Asp Arg Val Ser Ala Val Val           
    530                 535                 540                           

ggt gaa cag gta tgg aat ttc gcc gat ttt gcg acc tcg caa ggc ata       6479
Gly Glu Gln Val Trp Asn Phe Ala Asp Phe Ala Thr Ser Gln Gly Ile           
545                 550                 555                 560           

ttg cgc gtt ggc ggt aac aag aaa ggg atc ttc act cgc gac cgc aaa       6527
Leu Arg Val Gly Gly Asn Lys Lys Gly Ile Phe Thr Arg Asp Arg Lys           
                565                 570                 575               

ccg aag tcg gcg gct ttt ctg ctg caa aaa cgc tgg act ggc atg aac       6575
Pro Lys Ser Ala Ala Phe Leu Leu Gln Lys Arg Trp Thr Gly Met Asn           
            580                 585                 590                   

ttc ggt gaa aaa ccg cag cag gga ggc aaa caa tga atcaacaact            6621
Phe Gly Glu Lys Pro Gln Gln Gly Gly Lys Gln                               
        595                 600                                           

ctcctggcgc accatcgtcg gctacagcct cgggaattgc taccgagctc gaatttcccc     6681

gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg     6741

atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc     6801

atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac     6861

gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct     6921

atgttactag atcgggaatt cgtaatcatg gtcatagtcg agtcgacgtt ccttgacagg     6981

atatattggc gggtaaacta agtcgctgta tgtgtttgtt tgagatcctc tagggcatgc     7041

aggctcgcgg cggacgcacg acgccggggc gagaccatag gcgatctcct aaatcaatag     7101

tagctgtaac ctcgaagcgt ttcacttgta acaacgattg agaatttttg tcataaaatt     7161

gaaatacttg gttcgcattt ttgtcatccg cggtcagccg caattctgac gaactgccca     7221

tttagctgga gatgattgta catccttcac gtgaaaattt ctcaagcgct gtgaacaagg     7281

gttcagattt tagattgaaa ggtgagccgt tgaaacacgt tcttcttgtc gatgacgacg     7341

tcgctatgcg gcatcttatt attgaatacc ttacgatcca cgccttcaaa gtgaccgcgg     7401

tagccgacag cacccagttc acaagagtac tctcttccgc gacggtcgat gtcgtggttg     7461

ttgatctaaa tttaggtcgt gaagatgggc tcgagatcgt tcgtaatctg gcggcaaagt     7521

ctgatattcc aatcataatt atcagtggcg accgccttga ggagacggat aaagttgttg     7581

cactcgagct aggagcaagt gattttatcg ctaagccgtt cagtatcaga gagtttctag     7641

cacgcattcg ggttgccttg cgcgtgcgcc ccaacgttgt ccgctccaaa gaccgacggt     7701

ctttttgttt tactgactgg acacttaatc tcaggcaacg tcgcttgatg tccgaagctg     7761

gcggtgaggt gaaacttacg gcaggtgagt tcaatcttct cctcgcgttt ttagagaaac     7821

cccgcgacgt tctatcgcgc gagcaacttc tcattgccag tcgagtacgc gacgaggagg     7881

tttatgacag gagtatagat gttctcattt tgaggctgcg ccgcaaactt gaggcggatc     7941

cgtcaagccc tcaactgata aaaacagcaa gaggtgccgg ttatttcttt gacgcggacg     8001

tgcaggtttc gcacgggggg acgatggcag cctgagccaa ttgcatttgc ctcttaatta     8061

tctggctcaa agggtgactg aggagtaagc gatgtgccca tcacactgcg catgcaagct     8121

gatctggatc t                                                          8132


<210>  55
<211>  603
<212>  PRT
<213>  Hordeum vulgare

<400>  55

Met Leu Arg Pro Val Glu Thr Pro Thr Arg Glu Ile Lys Lys Leu Asp 
1               5                   10                  15      


Gly Leu Trp Ala Phe Ser Leu Asp Arg Glu Asn Cys Gly Ile Asp Gln 
            20                  25                  30          


Arg Trp Trp Glu Ser Ala Leu Gln Glu Ser Arg Ala Ile Ala Val Pro 
        35                  40                  45              


Gly Ser Phe Asn Asp Gln Phe Ala Asp Ala Asp Ile Arg Asn Tyr Ala 
    50                  55                  60                  


Gly Asn Val Trp Tyr Gln Arg Glu Val Phe Ile Pro Lys Gly Trp Ala 
65                  70                  75                  80  


Gly Gln Arg Ile Val Leu Arg Phe Asp Ala Val Thr His Tyr Gly Lys 
                85                  90                  95      


Val Trp Val Asn Asn Gln Glu Val Met Glu His Gln Gly Gly Tyr Thr 
            100                 105                 110         


Pro Phe Glu Ala Asp Val Thr Pro Tyr Val Ile Ala Gly Lys Ser Val 
        115                 120                 125             


Arg Ile Thr Val Cys Val Asn Asn Glu Leu Asn Trp Gln Thr Ile Pro 
    130                 135                 140                 


Pro Gly Met Val Ile Thr Asp Glu Asn Gly Lys Lys Lys Gln Ser Tyr 
145                 150                 155                 160 


Phe His Asp Phe Phe Asn Tyr Ala Gly Ile His Arg Ser Val Met Leu 
                165                 170                 175     


Tyr Thr Thr Pro Asn Thr Trp Val Asp Asp Ile Thr Val Val Thr His 
            180                 185                 190         


Val Ala Gln Asp Cys Asn His Ala Ser Val Asp Trp Gln Val Val Ala 
        195                 200                 205             


Asn Gly Asp Val Ser Val Glu Leu Arg Asp Ala Asp Gln Gln Val Val 
    210                 215                 220                 


Ala Thr Gly Gln Gly Thr Ser Gly Thr Leu Gln Val Val Asn Pro His 
225                 230                 235                 240 


Leu Trp Gln Pro Gly Glu Gly Tyr Leu Tyr Glu Leu Cys Val Thr Ala 
                245                 250                 255     


Lys Ser Gln Thr Glu Cys Asp Ile Tyr Pro Leu Arg Val Gly Ile Arg 
            260                 265                 270         


Ser Val Ala Val Lys Gly Glu Gln Phe Leu Ile Asn His Lys Pro Phe 
        275                 280                 285             


Tyr Phe Thr Gly Phe Gly Arg His Glu Asp Ala Asp Leu Arg Gly Lys 
    290                 295                 300                 


Gly Phe Asp Asn Val Leu Met Val His Asp His Ala Leu Met Asp Trp 
305                 310                 315                 320 


Ile Gly Ala Asn Ser Tyr Arg Thr Ser His Tyr Pro Tyr Ala Glu Glu 
                325                 330                 335     


Met Leu Asp Trp Ala Asp Glu His Gly Ile Val Val Ile Asp Glu Thr 
            340                 345                 350         


Ala Ala Val Gly Phe Asn Leu Ser Leu Gly Ile Gly Phe Glu Ala Gly 
        355                 360                 365             


Asn Lys Pro Lys Glu Leu Tyr Ser Glu Glu Ala Val Asn Gly Glu Thr 
    370                 375                 380                 


Gln Gln Ala His Leu Gln Ala Ile Lys Glu Leu Ile Ala Arg Asp Lys 
385                 390                 395                 400 


Asn His Pro Ser Val Val Met Trp Ser Ile Ala Asn Glu Pro Asp Thr 
                405                 410                 415     


Arg Pro Gln Gly Ala Arg Glu Tyr Phe Ala Pro Leu Ala Glu Ala Thr 
            420                 425                 430         


Arg Lys Leu Asp Pro Thr Arg Pro Ile Thr Cys Val Asn Val Met Phe 
        435                 440                 445             


Cys Asp Ala His Thr Asp Thr Ile Ser Asp Leu Phe Asp Val Leu Cys 
    450                 455                 460                 


Leu Asn Arg Tyr Tyr Gly Trp Tyr Val Gln Ser Gly Asp Leu Glu Thr 
465                 470                 475                 480 


Ala Glu Lys Val Leu Glu Lys Glu Leu Leu Ala Trp Gln Glu Lys Leu 
                485                 490                 495     


His Gln Pro Ile Ile Ile Thr Glu Tyr Gly Val Asp Thr Leu Ala Gly 
            500                 505                 510         


Leu His Ser Met Tyr Thr Asp Met Trp Ser Glu Glu Tyr Gln Cys Ala 
        515                 520                 525             


Trp Leu Asp Met Tyr His Arg Val Phe Asp Arg Val Ser Ala Val Val 
    530                 535                 540                 


Gly Glu Gln Val Trp Asn Phe Ala Asp Phe Ala Thr Ser Gln Gly Ile 
545                 550                 555                 560 


Leu Arg Val Gly Gly Asn Lys Lys Gly Ile Phe Thr Arg Asp Arg Lys 
                565                 570                 575     


Pro Lys Ser Ala Ala Phe Leu Leu Gln Lys Arg Trp Thr Gly Met Asn 
            580                 585                 590         


Phe Gly Glu Lys Pro Gln Gln Gly Gly Lys Gln 
        595                 600             


<210>  56
<211>  39
<212>  DNA
<213>  Hordeum vulgare


<220>
<221>  Kill triad Fw primer
<222>  (1)..(39)
<223>  Kill triad Fw primer to randomise mutant enhancer

<400>  56
gcatacgaag catagtacga cgtaggcgtc caaactttg                              39


<210>  57
<211>  48
<212>  DNA
<213>  Hordeum vulgare


<220>
<221>  Kill triad Rv primer
<222>  (1)..(48)
<223>  Kill triad Rv primer to randomise mutant enhancer

<400>  57
tcgtactatg cttcgtatgc ctacaaaatg tagcttgaaa ttaaagag                    48


<210>  58
<211>  8132
<212>  DNA
<213>  Hordeum vulgare


<220>
<221>  pCLEAN-G185-KOtriad-ProGUS
<222>  (1)..(8132)

<220>
<221>  Vector backbone, pCLEAN-G185
<222>  (1)..(2599)

<220>
<221>  KOtriad HvPAPhy_a promoter
<222>  (2600)..(4799)

<220>
<221>  Randomized sequence replacing the enhancer triad (GCN4, skn1, 
       RY-element)
<222>  (4561)..(4580)

<220>
<221>  CDS
<222>  (4800)..(6611)
<223>  UidA gene (encoding GUS)

<220>
<221>  NOS terminator
<222>  (6612)..(6959)

<220>
<221>  Vector backbone, pCLEAN-G185
<222>  (6960)..(8132)

<400>  58
catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt       60

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg      120

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg      180

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag      240

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc      300

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa      360

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg      420

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc      480

taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac      540

cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg      600

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt      660

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt      720

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa      780

atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat      840

caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg      900

tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta      960

tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa     1020

aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa     1080

aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa     1140

atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac     1200

gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac     1260

tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc     1320

tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg     1380

cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt     1440

aacaacattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt     1500

cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata     1560

cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg     1620

ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt     1680

tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg     1740

gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga     1800

caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa     1860

caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc     1920

gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata     1980

gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt     2040

ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa     2100

tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga     2160

gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt     2220

tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt     2280

cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag     2340

cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt     2400

ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct     2460

ggatttccga tccccggaat tagatccgtt taaactacgt aagatcttgg caggatatat     2520

tgtggtgtaa acgttcctgc ggcggtcgag atggatcttg gcaggatata ttgtggtgta     2580

aacgttcctg cggccgcatc ttgggcaaca tatcaggggc agcgccattg ccctgcgact     2640

gacggcggcg gtggaggagc ttggggcaga catgagctga gaacgacgag agagaggagt     2700

ggtggcgggc gagacagagg agcgacatga ttgaagaaga gcagcgggat tgaggattag     2760

ggattcctgc gattttacac ttgacctctc cataaaagat tggcctaatc gaagctgaga     2820

acgtggaggt caacaagtgg tcaaacgagc ctgtacgcac cgcatacgag caacagtgat     2880

cggattttca cgtcacatcg tatatagtga tcgtaaaagc catattctaa agttggatga     2940

ccgtattgtg cttccatgtc aactgcaagg accgtgagtg tatttatctc taaaatataa     3000

atcaaatata atggtgggct tcttcagacc tcattcagct caaaatccgc ccatgaagcc     3060

aatttgaatg ctctaacttt tgcgaagctg gaatctattg tatccaagac tagtgttttc     3120

aaagatagtc ttttcggaat ttcaaaatca ctatgtgtgt tcgtcgtatg aacaactctc     3180

gactagagag tattcttaat aatgcgataa gagtataaca taacaatttt tttgaatgta     3240

ggtttagttg gtctccatgg agcgagacct agtcctattt ggggaggcct ctcgtagttt     3300

ggatggacat agttctgtcg gttcaggttg taatgcaact cctcgcactt gactctaaaa     3360

tgacttaggc tattgaaagg cccaaaaaca atagtattct tcccttgagt tcattcaagt     3420

gagagtatat gtccatgggg ctaagagaag gggtcccttg acaaatgatc aaggttgcgc     3480

aaggtgatga taggtatatt ggtaaatctg tatccacgag ttcatgaagt attcaaaggt     3540

gggggtctat agtccatggt acatcaatca tttccaccca tgtatgaatg ggcctttggc     3600

tagttgggct catttagatt tgggctcagt tacttcttgc cttaaatatt gactttgaca     3660

tttcattttg gtttcccttt ttatttgttg accatgataa tttgcttggc cttttattta     3720

attttttatg gttttgatta ttttttaaac acaatacaga cgaaaacatt catgtacaca     3780

cacatgcatt catctttatg aacatacaca tccacatcat gtccctatca tcttgaaatt     3840

tatgaagtca tagtagacac ctagtcgtcg aggggaattc tcctcggatt gaatgtgtat     3900

cgtcgaaaat tgtgaaataa atgtgagcgc caggacttga atcttgatgg actaggataa     3960

cacagtttct ctaaccatcc aaccgtatgt tggttcgcga tagtttggat tgcttaccac     4020

atgtgtcatg tggttgctag gacttccatt aatctggccg aaccttgtta attgagttgg     4080

atatttcttg accattttag accttattaa gagcatcttc aacaacagtg taaaaaatcc     4140

gcgcccaata aatttttagc gcgccactgt agcactttta aagcgcgggg acggaagcat     4200

cttcaataga cacgcgctaa tgcggcgccc aatccacccc ggtccagcga cccacccaca     4260

acagctcaga gtttgctgcg cgcgcaagcc ggcacaccaa atatgctgtc cgcgatagcg     4320

tttttaagcg cgtgcccaaa attttttagt gcgagcacag ttttgcagcg tctgttggag     4380

ctgttcggcg ccaaaaaacg aatcttttaa cgcgcggtgt agttttgagg cgtctgttgg     4440

agatggtcta accggcttga accgtgttga aaaaaaaacc cggcttgaat ttttgttgac     4500

gagccatagt tgcatattac gtacgttact tgctctttaa tttcaagcta cattttgtag     4560

gcatacgaag catagtacga cgtaggcgtc caaactttgg ctagcgcagc tgcatgcacg     4620

tccacaaggc accaaaggcg caggcggcaa ctttgctcgt ttattttctt gcgggtccaa     4680

gatgagttcc agaccatgga cgaattccac ttcgggctcc caatctcctc tgccggattc     4740

ctataagttt cctgccaaga agcatcccaa tcccctcaat gccaagcaac aacatcaac      4799

atg tta cgt cct gta gaa acc cca acc cgt gaa atc aaa aaa ctc gac       4847
Met Leu Arg Pro Val Glu Thr Pro Thr Arg Glu Ile Lys Lys Leu Asp           
1               5                   10                  15                

ggc ctg tgg gca ttc agt ctg gat cgc gaa aac tgt gga att gat cag       4895
Gly Leu Trp Ala Phe Ser Leu Asp Arg Glu Asn Cys Gly Ile Asp Gln           
            20                  25                  30                    

cgt tgg tgg gaa agc gcg tta caa gaa agc cgg gca att gct gtg cca       4943
Arg Trp Trp Glu Ser Ala Leu Gln Glu Ser Arg Ala Ile Ala Val Pro           
        35                  40                  45                        

ggc agt ttt aac gat cag ttc gcc gat gca gat att cgt aat tat gcg       4991
Gly Ser Phe Asn Asp Gln Phe Ala Asp Ala Asp Ile Arg Asn Tyr Ala           
    50                  55                  60                            

ggc aac gtc tgg tat cag cgc gaa gtc ttt ata ccg aaa ggt tgg gca       5039
Gly Asn Val Trp Tyr Gln Arg Glu Val Phe Ile Pro Lys Gly Trp Ala           
65                  70                  75                  80            

ggc cag cgt atc gtg ctg cgt ttc gat gcg gtc act cat tac ggc aaa       5087
Gly Gln Arg Ile Val Leu Arg Phe Asp Ala Val Thr His Tyr Gly Lys           
                85                  90                  95                

gtg tgg gtc aat aat cag gaa gtg atg gag cat cag ggc ggc tat acg       5135
Val Trp Val Asn Asn Gln Glu Val Met Glu His Gln Gly Gly Tyr Thr           
            100                 105                 110                   

cca ttt gaa gcc gat gtc acg ccg tat gtt att gcc ggg aaa agt gta       5183
Pro Phe Glu Ala Asp Val Thr Pro Tyr Val Ile Ala Gly Lys Ser Val           
        115                 120                 125                       

cgt atc acc gtt tgt gtg aac aac gaa ctg aac tgg cag act atc ccg       5231
Arg Ile Thr Val Cys Val Asn Asn Glu Leu Asn Trp Gln Thr Ile Pro           
    130                 135                 140                           

ccg gga atg gtg att acc gac gaa aac ggc aag aaa aag cag tct tac       5279
Pro Gly Met Val Ile Thr Asp Glu Asn Gly Lys Lys Lys Gln Ser Tyr           
145                 150                 155                 160           

ttc cat gat ttc ttt aac tat gcc gga atc cat cgc agc gta atg ctc       5327
Phe His Asp Phe Phe Asn Tyr Ala Gly Ile His Arg Ser Val Met Leu           
                165                 170                 175               

tac acc acg ccg aac acc tgg gtg gac gat atc acc gtg gtg acg cat       5375
Tyr Thr Thr Pro Asn Thr Trp Val Asp Asp Ile Thr Val Val Thr His           
            180                 185                 190                   

gtc gcg caa gac tgt aac cac gcg tct gtt gac tgg cag gtg gtg gcc       5423
Val Ala Gln Asp Cys Asn His Ala Ser Val Asp Trp Gln Val Val Ala           
        195                 200                 205                       

aat ggt gat gtc agc gtt gaa ctg cgt gat gcg gat caa cag gtg gtt       5471
Asn Gly Asp Val Ser Val Glu Leu Arg Asp Ala Asp Gln Gln Val Val           
    210                 215                 220                           

gca act gga caa ggc act agc ggg act ttg caa gtg gtg aat ccg cac       5519
Ala Thr Gly Gln Gly Thr Ser Gly Thr Leu Gln Val Val Asn Pro His           
225                 230                 235                 240           

ctc tgg caa ccg ggt gaa ggt tat ctc tat gaa ctg tgc gtc aca gcc       5567
Leu Trp Gln Pro Gly Glu Gly Tyr Leu Tyr Glu Leu Cys Val Thr Ala           
                245                 250                 255               

aaa agc cag aca gag tgt gat atc tac ccg ctt cgc gtc ggc atc cgg       5615
Lys Ser Gln Thr Glu Cys Asp Ile Tyr Pro Leu Arg Val Gly Ile Arg           
            260                 265                 270                   

tca gtg gca gtg aag ggc gaa cag ttc ctg att aac cac aaa ccg ttc       5663
Ser Val Ala Val Lys Gly Glu Gln Phe Leu Ile Asn His Lys Pro Phe           
        275                 280                 285                       

tac ttt act ggc ttt ggt cgt cat gaa gat gcg gac ttg cgt ggc aaa       5711
Tyr Phe Thr Gly Phe Gly Arg His Glu Asp Ala Asp Leu Arg Gly Lys           
    290                 295                 300                           

gga ttc gat aac gtg ctg atg gtg cac gac cac gca tta atg gac tgg       5759
Gly Phe Asp Asn Val Leu Met Val His Asp His Ala Leu Met Asp Trp           
305                 310                 315                 320           

att ggg gcc aac tcc tac cgt acc tcg cat tac cct tac gct gaa gag       5807
Ile Gly Ala Asn Ser Tyr Arg Thr Ser His Tyr Pro Tyr Ala Glu Glu           
                325                 330                 335               

atg ctc gac tgg gca gat gaa cat ggc atc gtg gtg att gat gaa act       5855
Met Leu Asp Trp Ala Asp Glu His Gly Ile Val Val Ile Asp Glu Thr           
            340                 345                 350                   

gct gct gtc ggc ttt aac ctc tct tta ggc att ggt ttc gaa gcg ggc       5903
Ala Ala Val Gly Phe Asn Leu Ser Leu Gly Ile Gly Phe Glu Ala Gly           
        355                 360                 365                       

aac aag ccg aaa gaa ctg tac agc gaa gag gca gtc aac ggg gaa act       5951
Asn Lys Pro Lys Glu Leu Tyr Ser Glu Glu Ala Val Asn Gly Glu Thr           
    370                 375                 380                           

cag caa gcg cac tta cag gcg att aaa gag ctg ata gcg cgt gac aaa       5999
Gln Gln Ala His Leu Gln Ala Ile Lys Glu Leu Ile Ala Arg Asp Lys           
385                 390                 395                 400           

aac cac cca agc gtg gtg atg tgg agt att gcc aac gaa ccg gat acc       6047
Asn His Pro Ser Val Val Met Trp Ser Ile Ala Asn Glu Pro Asp Thr           
                405                 410                 415               

cgt ccg caa ggt gca cgg gaa tat ttc gcg cca ctg gcg gaa gca acg       6095
Arg Pro Gln Gly Ala Arg Glu Tyr Phe Ala Pro Leu Ala Glu Ala Thr           
            420                 425                 430                   

cgt aaa ctc gac ccg acg cgt ccg atc acc tgc gtc aat gta atg ttc       6143
Arg Lys Leu Asp Pro Thr Arg Pro Ile Thr Cys Val Asn Val Met Phe           
        435                 440                 445                       

tgc gac gct cac acc gat acc atc agc gat ctc ttt gat gtg ctg tgc       6191
Cys Asp Ala His Thr Asp Thr Ile Ser Asp Leu Phe Asp Val Leu Cys           
    450                 455                 460                           

ctg aac cgt tat tac gga tgg tat gtc caa agc ggc gat ttg gaa acg       6239
Leu Asn Arg Tyr Tyr Gly Trp Tyr Val Gln Ser Gly Asp Leu Glu Thr           
465                 470                 475                 480           

gca gag aag gta ctg gaa aaa gaa ctt ctg gcc tgg cag gag aaa ctg       6287
Ala Glu Lys Val Leu Glu Lys Glu Leu Leu Ala Trp Gln Glu Lys Leu           
                485                 490                 495               

cat cag ccg att atc atc acc gaa tac ggc gtg gat acg tta gcc ggg       6335
His Gln Pro Ile Ile Ile Thr Glu Tyr Gly Val Asp Thr Leu Ala Gly           
            500                 505                 510                   

ctg cac tca atg tac acc gac atg tgg agt gaa gag tat cag tgt gca       6383
Leu His Ser Met Tyr Thr Asp Met Trp Ser Glu Glu Tyr Gln Cys Ala           
        515                 520                 525                       

tgg ctg gat atg tat cac cgc gtc ttt gat cgc gtc agc gcc gtc gtc       6431
Trp Leu Asp Met Tyr His Arg Val Phe Asp Arg Val Ser Ala Val Val           
    530                 535                 540                           

ggt gaa cag gta tgg aat ttc gcc gat ttt gcg acc tcg caa ggc ata       6479
Gly Glu Gln Val Trp Asn Phe Ala Asp Phe Ala Thr Ser Gln Gly Ile           
545                 550                 555                 560           

ttg cgc gtt ggc ggt aac aag aaa ggg atc ttc act cgc gac cgc aaa       6527
Leu Arg Val Gly Gly Asn Lys Lys Gly Ile Phe Thr Arg Asp Arg Lys           
                565                 570                 575               

ccg aag tcg gcg gct ttt ctg ctg caa aaa cgc tgg act ggc atg aac       6575
Pro Lys Ser Ala Ala Phe Leu Leu Gln Lys Arg Trp Thr Gly Met Asn           
            580                 585                 590                   

ttc ggt gaa aaa ccg cag cag gga ggc aaa caa tga atcaacaact            6621
Phe Gly Glu Lys Pro Gln Gln Gly Gly Lys Gln                               
        595                 600                                           

ctcctggcgc accatcgtcg gctacagcct cgggaattgc taccgagctc gaatttcccc     6681

gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg     6741

atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc     6801

atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac     6861

gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct     6921

atgttactag atcgggaatt cgtaatcatg gtcatagtcg agtcgacgtt ccttgacagg     6981

atatattggc gggtaaacta agtcgctgta tgtgtttgtt tgagatcctc tagggcatgc     7041

aggctcgcgg cggacgcacg acgccggggc gagaccatag gcgatctcct aaatcaatag     7101

tagctgtaac ctcgaagcgt ttcacttgta acaacgattg agaatttttg tcataaaatt     7161

gaaatacttg gttcgcattt ttgtcatccg cggtcagccg caattctgac gaactgccca     7221

tttagctgga gatgattgta catccttcac gtgaaaattt ctcaagcgct gtgaacaagg     7281

gttcagattt tagattgaaa ggtgagccgt tgaaacacgt tcttcttgtc gatgacgacg     7341

tcgctatgcg gcatcttatt attgaatacc ttacgatcca cgccttcaaa gtgaccgcgg     7401

tagccgacag cacccagttc acaagagtac tctcttccgc gacggtcgat gtcgtggttg     7461

ttgatctaaa tttaggtcgt gaagatgggc tcgagatcgt tcgtaatctg gcggcaaagt     7521

ctgatattcc aatcataatt atcagtggcg accgccttga ggagacggat aaagttgttg     7581

cactcgagct aggagcaagt gattttatcg ctaagccgtt cagtatcaga gagtttctag     7641

cacgcattcg ggttgccttg cgcgtgcgcc ccaacgttgt ccgctccaaa gaccgacggt     7701

ctttttgttt tactgactgg acacttaatc tcaggcaacg tcgcttgatg tccgaagctg     7761

gcggtgaggt gaaacttacg gcaggtgagt tcaatcttct cctcgcgttt ttagagaaac     7821

cccgcgacgt tctatcgcgc gagcaacttc tcattgccag tcgagtacgc gacgaggagg     7881

tttatgacag gagtatagat gttctcattt tgaggctgcg ccgcaaactt gaggcggatc     7941

cgtcaagccc tcaactgata aaaacagcaa gaggtgccgg ttatttcttt gacgcggacg     8001

tgcaggtttc gcacgggggg acgatggcag cctgagccaa ttgcatttgc ctcttaatta     8061

tctggctcaa agggtgactg aggagtaagc gatgtgccca tcacactgcg catgcaagct     8121

gatctggatc t                                                          8132


<210>  59
<211>  603
<212>  PRT
<213>  Hordeum vulgare

<400>  59

Met Leu Arg Pro Val Glu Thr Pro Thr Arg Glu Ile Lys Lys Leu Asp 
1               5                   10                  15      


Gly Leu Trp Ala Phe Ser Leu Asp Arg Glu Asn Cys Gly Ile Asp Gln 
            20                  25                  30          


Arg Trp Trp Glu Ser Ala Leu Gln Glu Ser Arg Ala Ile Ala Val Pro 
        35                  40                  45              


Gly Ser Phe Asn Asp Gln Phe Ala Asp Ala Asp Ile Arg Asn Tyr Ala 
    50                  55                  60                  


Gly Asn Val Trp Tyr Gln Arg Glu Val Phe Ile Pro Lys Gly Trp Ala 
65                  70                  75                  80  


Gly Gln Arg Ile Val Leu Arg Phe Asp Ala Val Thr His Tyr Gly Lys 
                85                  90                  95      


Val Trp Val Asn Asn Gln Glu Val Met Glu His Gln Gly Gly Tyr Thr 
            100                 105                 110         


Pro Phe Glu Ala Asp Val Thr Pro Tyr Val Ile Ala Gly Lys Ser Val 
        115                 120                 125             


Arg Ile Thr Val Cys Val Asn Asn Glu Leu Asn Trp Gln Thr Ile Pro 
    130                 135                 140                 


Pro Gly Met Val Ile Thr Asp Glu Asn Gly Lys Lys Lys Gln Ser Tyr 
145                 150                 155                 160 


Phe His Asp Phe Phe Asn Tyr Ala Gly Ile His Arg Ser Val Met Leu 
                165                 170                 175     


Tyr Thr Thr Pro Asn Thr Trp Val Asp Asp Ile Thr Val Val Thr His 
            180                 185                 190         


Val Ala Gln Asp Cys Asn His Ala Ser Val Asp Trp Gln Val Val Ala 
        195                 200                 205             


Asn Gly Asp Val Ser Val Glu Leu Arg Asp Ala Asp Gln Gln Val Val 
    210                 215                 220                 


Ala Thr Gly Gln Gly Thr Ser Gly Thr Leu Gln Val Val Asn Pro His 
225                 230                 235                 240 


Leu Trp Gln Pro Gly Glu Gly Tyr Leu Tyr Glu Leu Cys Val Thr Ala 
                245                 250                 255     


Lys Ser Gln Thr Glu Cys Asp Ile Tyr Pro Leu Arg Val Gly Ile Arg 
            260                 265                 270         


Ser Val Ala Val Lys Gly Glu Gln Phe Leu Ile Asn His Lys Pro Phe 
        275                 280                 285             


Tyr Phe Thr Gly Phe Gly Arg His Glu Asp Ala Asp Leu Arg Gly Lys 
    290                 295                 300                 


Gly Phe Asp Asn Val Leu Met Val His Asp His Ala Leu Met Asp Trp 
305                 310                 315                 320 


Ile Gly Ala Asn Ser Tyr Arg Thr Ser His Tyr Pro Tyr Ala Glu Glu 
                325                 330                 335     


Met Leu Asp Trp Ala Asp Glu His Gly Ile Val Val Ile Asp Glu Thr 
            340                 345                 350         


Ala Ala Val Gly Phe Asn Leu Ser Leu Gly Ile Gly Phe Glu Ala Gly 
        355                 360                 365             


Asn Lys Pro Lys Glu Leu Tyr Ser Glu Glu Ala Val Asn Gly Glu Thr 
    370                 375                 380                 


Gln Gln Ala His Leu Gln Ala Ile Lys Glu Leu Ile Ala Arg Asp Lys 
385                 390                 395                 400 


Asn His Pro Ser Val Val Met Trp Ser Ile Ala Asn Glu Pro Asp Thr 
                405                 410                 415     


Arg Pro Gln Gly Ala Arg Glu Tyr Phe Ala Pro Leu Ala Glu Ala Thr 
            420                 425                 430         


Arg Lys Leu Asp Pro Thr Arg Pro Ile Thr Cys Val Asn Val Met Phe 
        435                 440                 445             


Cys Asp Ala His Thr Asp Thr Ile Ser Asp Leu Phe Asp Val Leu Cys 
    450                 455                 460                 


Leu Asn Arg Tyr Tyr Gly Trp Tyr Val Gln Ser Gly Asp Leu Glu Thr 
465                 470                 475                 480 


Ala Glu Lys Val Leu Glu Lys Glu Leu Leu Ala Trp Gln Glu Lys Leu 
                485                 490                 495     


His Gln Pro Ile Ile Ile Thr Glu Tyr Gly Val Asp Thr Leu Ala Gly 
            500                 505                 510         


Leu His Ser Met Tyr Thr Asp Met Trp Ser Glu Glu Tyr Gln Cys Ala 
        515                 520                 525             


Trp Leu Asp Met Tyr His Arg Val Phe Asp Arg Val Ser Ala Val Val 
    530                 535                 540                 


Gly Glu Gln Val Trp Asn Phe Ala Asp Phe Ala Thr Ser Gln Gly Ile 
545                 550                 555                 560 


Leu Arg Val Gly Gly Asn Lys Lys Gly Ile Phe Thr Arg Asp Arg Lys 
                565                 570                 575     


Pro Lys Ser Ala Ala Phe Leu Leu Gln Lys Arg Trp Thr Gly Met Asn 
            580                 585                 590         


Phe Gly Glu Lys Pro Gln Gln Gly Gly Lys Gln 
        595                 600             


