                         SEQUENCE LISTING

<110>  Universiteit Utrecht Holding BV
       The Rockefeller University
       Fischetti, Vincent A.
       Schmitz, Jonathan
       Gilmer, Daniel
 
<120>  STREPTOCOCCUS BACTERIOPHAGE LYSINS FOR TREATMENT OF GRAM POSITIVE
        BACTERIA IN COMPANION ANIMALS AND LIVESTOCK

<130>  600-1-302PCT

<140>  PCT/US 12/034356
<141>  2012-04-20

<150>  61/477,909
<151>  2011-04-21

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  452
<212>  PRT
<213>  Streptococcus suis

<400>  1

Met Thr Ile Asn Leu Glu Thr Ser Ile Arg Trp Met Ser Asp Arg Val 
1               5                   10                  15      


Gly Lys Val Ser Tyr Ser Met Asp Tyr Arg Asn Gly Pro Asn Ser Tyr 
            20                  25                  30          


Asp Cys Ser Ser Ala Val Tyr Tyr Ala Leu Met Ala Gly Gly Ala Ile 
        35                  40                  45              


Ser Ala Gly Trp Ala Val Asn Thr Glu Tyr Met His Asp Trp Leu Ile 
    50                  55                  60                  


Arg Asn Gly Tyr Val Leu Val Ala Glu Asn Lys Pro Phe Asn Ala Gln 
65                  70                  75                  80  


Arg His Asp Val Cys Ile Leu Gly Lys Arg Gly Tyr Ser Ser Gly Ala 
                85                  90                  95      


Gly Gly His Val Val Ile Phe Val Asp Asn Val Asn Val Ile His Cys 
            100                 105                 110         


Asn Tyr Ala Arg Asn Gly Ile Ser Ile Asp Asn Tyr Asn Gln Val His 
        115                 120                 125             


Arg Gly Met Tyr Tyr Tyr Leu Tyr Arg Pro Ala Asn Gln Pro Ser Ile 
    130                 135                 140                 


Ser Asn Lys Ser Leu Asp Gln Leu Val Lys Glu Thr Leu Ala Gly Val 
145                 150                 155                 160 


His Gly Asn Gly Asp Thr Arg Lys Ala Ser Leu Gly Ser Gln Tyr Glu 
                165                 170                 175     


Ala Val Met Ala Val Ile Asn Gly Lys Ala Ser Ala Ser Glu Lys Ser 
            180                 185                 190         


Asp Glu Glu Leu Ala Arg Glu Val Leu Ala Gly Lys His Gly Ala Gly 
        195                 200                 205             


Glu Asp Arg Lys Arg Ser Leu Gly Pro Arg Tyr Glu Pro Val Gln Ala 
    210                 215                 220                 


Lys Val Asn Glu Leu Leu Lys Ala Lys Glu Lys Pro Ser Glu Thr Ala 
225                 230                 235                 240 


Lys Asn Glu Pro Gln Thr Val Gln Phe Lys Glu Asp Gly Asp Leu Ser 
                245                 250                 255     


Phe Asn Gly Ala Ile Leu Lys Lys Ser Val Leu Glu Ile Ile Leu Lys 
            260                 265                 270         


Lys Cys Lys Glu His Asp Ile Leu Pro Ser Tyr Ala Leu Thr Ile Leu 
        275                 280                 285             


His Tyr Glu Gly Leu Trp Gly Thr Ser Ala Val Gly Lys Ala Asp Asn 
    290                 295                 300                 


Asn Trp Gly Gly Met Thr Trp Thr Gly Gln Gly Asn Arg Pro Ser Gly 
305                 310                 315                 320 


Val Ile Val Thr Gln Gly Leu Ala Arg Pro Ser Asn Glu Gly Gly His 
                325                 330                 335     


Tyr Met His Tyr Ala Thr Val Asp Asp Phe Leu Thr Asp Trp Phe Tyr 
            340                 345                 350         


Leu Leu Arg Lys Asp Gly Ser Tyr Lys Val Ser Gly Ala Leu Thr Phe 
        355                 360                 365             


Ser Glu Ser Ile Lys Gly Met Phe Gln Val Gly Gly Ala Lys Tyr Asp 
    370                 375                 380                 


Tyr Ala Ala Ala Gly Tyr Asp Ser Tyr Leu Val Gly Ala Thr Ser Arg 
385                 390                 395                 400 


Leu Lys Ala Ile Glu Ser Glu Asn Gly Ser Leu Thr Arg Phe Asp Ala 
                405                 410                 415     


Thr Ser Asn Asn Val His Ser Val Asp Pro Asp Lys Ile Ser Val Asp 
            420                 425                 430         


Ile Asp Gly Ile Glu Val Thr Ile Asn Gly Val Val Tyr Lys Leu Glu 
        435                 440                 445             


Lys Lys Pro Val 
    450         


<210>  2
<211>  255
<212>  PRT
<213>  Streptococcus suis

<400>  2

Met Thr Ile Asn Leu Glu Thr Ser Ile Arg Trp Met Ser Asp Arg Val 
1               5                   10                  15      


Gly Lys Val Ser Tyr Ser Met Asp Tyr Arg Asn Gly Pro Asn Ser Tyr 
            20                  25                  30          


Asp Cys Ser Ser Ala Val Tyr Tyr Ala Leu Met Ala Gly Gly Ala Ile 
        35                  40                  45              


Ser Ala Gly Trp Ala Val Asn Thr Glu Tyr Met His Asp Trp Leu Ile 
    50                  55                  60                  


Arg Asn Gly Tyr Val Leu Val Ala Glu Asn Lys Pro Phe Asn Ala Gln 
65                  70                  75                  80  


Arg His Asp Val Cys Ile Leu Gly Lys Arg Gly Tyr Ser Ser Gly Ala 
                85                  90                  95      


Gly Gly His Val Val Ile Phe Val Asp Asn Val Asn Val Ile His Cys 
            100                 105                 110         


Asn Tyr Ala Arg Asn Gly Ile Ser Ile Asp Asn Tyr Asn Gln Val His 
        115                 120                 125             


Arg Gly Met Tyr Tyr Tyr Leu Tyr Arg Pro Ala Asn Gln Pro Ser Ile 
    130                 135                 140                 


Ser Asn Lys Ser Leu Asp Gln Leu Val Lys Glu Thr Leu Ala Gly Val 
145                 150                 155                 160 


His Gly Asn Gly Asp Thr Arg Lys Ala Ser Leu Gly Ser Gln Tyr Glu 
                165                 170                 175     


Ala Val Met Ala Val Ile Asn Gly Lys Ala Ser Ala Ser Glu Lys Ser 
            180                 185                 190         


Asp Glu Glu Leu Ala Arg Glu Val Leu Ala Gly Lys His Gly Ala Gly 
        195                 200                 205             


Glu Asp Arg Lys Arg Ser Leu Gly Pro Arg Tyr Glu Pro Val Gln Ala 
    210                 215                 220                 


Lys Val Asn Glu Leu Leu Lys Ala Lys Glu Lys Pro Ser Glu Thr Ala 
225                 230                 235                 240 


Lys Asn Glu Pro Gln Thr Val Gln Phe Lys Glu Asp Gly Asp Leu 
                245                 250                 255 


<210>  3
<211>  245
<212>  PRT
<213>  Streptococcus suis

<400>  3

Met Thr Thr Val Asn Glu Ala Leu Asn Asn Val Arg Ala Gln Val Gly 
1               5                   10                  15      


Ser Gly Val Ser Val Gly Asn Gly Glu Cys Tyr Ala Leu Ala Ser Trp 
            20                  25                  30          


Tyr Glu Arg Met Ile Ser Pro Asp Ala Thr Val Gly Leu Gly Ala Gly 
        35                  40                  45              


Val Gly Trp Val Ser Gly Ala Ile Gly Asp Thr Ile Ser Ala Lys Asn 
    50                  55                  60                  


Ile Gly Ser Ser Tyr Asn Trp Gln Ala Asn Gly Trp Thr Val Ser Thr 
65                  70                  75                  80  


Ser Gly Pro Phe Lys Ala Gly Gln Ile Val Thr Leu Gly Ala Thr Pro 
                85                  90                  95      


Gly Asn Pro Tyr Gly His Val Val Ile Val Glu Ala Val Asp Gly Asp 
            100                 105                 110         


Arg Leu Thr Ile Leu Glu Gln Asn Tyr Gly Gly Lys Arg Tyr Pro Val 
        115                 120                 125             


Arg Asn Tyr Tyr Ser Ala Ala Ser Tyr Arg Gln Gln Val Val His Tyr 
    130                 135                 140                 


Ile Thr Pro Pro Gly Thr Val Ala Gln Ser Ala Pro Asn Leu Ala Gly 
145                 150                 155                 160 


Ser Arg Ser Tyr Arg Glu Thr Gly Thr Met Thr Val Thr Val Asp Ala 
                165                 170                 175     


Leu Asn Val Arg Arg Ala Pro Asn Thr Ser Gly Glu Ile Val Ala Val 
            180                 185                 190         


Tyr Lys Arg Gly Glu Ser Phe Asp Tyr Asp Thr Val Ile Ile Asp Val 
        195                 200                 205             


Asn Gly Tyr Val Trp Val Ser Tyr Ile Gly Gly Ser Gly Lys Arg Asn 
    210                 215                 220                 


Tyr Val Ala Thr Gly Ala Thr Lys Asp Gly Lys Arg Phe Gly Asn Ala 
225                 230                 235                 240 


Trp Gly Thr Phe Lys 
                245 


<210>  4
<211>  139
<212>  PRT
<213>  Streptococcus suis


<220>
<221>  CHAP DOMAIN
<222>  (1)..(139)

<400>  4

Leu Asn Asn Val Arg Ala Gln Val Gly Ser Gly Val Ser Val Gly Asn 
1               5                   10                  15      


Gly Glu Cys Tyr Ala Leu Ala Ser Trp Tyr Glu Arg Met Ile Ser Pro 
            20                  25                  30          


Asp Ala Thr Val Gly Leu Gly Ala Gly Val Gly Trp Val Ser Gly Ala 
        35                  40                  45              


Ile Gly Asp Thr Ile Ser Ala Lys Asn Ile Gly Ser Ser Tyr Asn Trp 
    50                  55                  60                  


Gln Ala Asn Gly Trp Thr Val Ser Thr Ser Gly Pro Phe Lys Ala Gly 
65                  70                  75                  80  


Gln Ile Val Thr Leu Gly Ala Thr Pro Gly Asn Pro Tyr Gly His Val 
                85                  90                  95      


Val Ile Val Glu Ala Val Asp Gly Asp Arg Leu Thr Ile Leu Glu Gln 
            100                 105                 110         


Asn Tyr Gly Gly Lys Arg Tyr Pro Val Arg Asn Tyr Tyr Ser Ala Ala 
        115                 120                 125             


Ser Tyr Arg Gln Gln Val Val His Tyr Ile Thr 
    130                 135                 


<210>  5
<211>  68
<212>  PRT
<213>  Streptococcus suis


<220>
<221>  SH3 DOMAIN
<222>  (1)..(67)

<220>
<221>  SH3 DOMAIN
<222>  (1)..(68)

<400>  5

Ser Arg Ser Tyr Arg Glu Thr Gly Thr Met Thr Val Thr Val Asp Ala 
1               5                   10                  15      


Leu Asn Val Arg Arg Ala Pro Asn Thr Ser Gly Glu Ile Val Ala Val 
            20                  25                  30          


Tyr Lys Arg Gly Glu Ser Phe Asp Tyr Asp Thr Val Ile Ile Asp Val 
        35                  40                  45              


Asn Gly Tyr Val Trp Val Ser Tyr Ile Gly Gly Ser Gly Lys Arg Asn 
    50                  55                  60                  


Tyr Val Ala Thr 
65              


<210>  6
<211>  137
<212>  PRT
<213>  Streptococcus suis


<220>
<221>  ENDOPEPTIDASE DOMAIN
<222>  (1)..(137)

<400>  6

Leu Glu Thr Ser Ile Arg Trp Met Ser Asp Arg Val Gly Lys Val Ser 
1               5                   10                  15      


Tyr Ser Met Asp Tyr Arg Asn Gly Pro Asn Ser Tyr Asp Cys Ser Ser 
            20                  25                  30          


Ala Val Tyr Tyr Ala Leu Met Ala Gly Gly Ala Ile Ser Ala Gly Trp 
        35                  40                  45              


Ala Val Asn Thr Glu Tyr Met His Asp Trp Leu Ile Arg Asn Gly Tyr 
    50                  55                  60                  


Val Leu Val Ala Glu Asn Lys Pro Phe Asn Ala Gln Arg His Asp Val 
65                  70                  75                  80  


Cys Ile Leu Gly Lys Arg Gly Tyr Ser Ser Gly Ala Gly Gly His Val 
                85                  90                  95      


Val Ile Phe Val Asp Asn Val Asn Val Ile His Cys Asn Tyr Ala Arg 
            100                 105                 110         


Asn Gly Ile Ser Ile Asp Asn Tyr Asn Gln Val His Arg Gly Met Tyr 
        115                 120                 125             


Tyr Tyr Leu Tyr Arg Pro Ala Asn Gln 
    130                 135         


<210>  7
<211>  82
<212>  PRT
<213>  Streptococcus suis


<220>
<221>  DUAL CPL-7 DOMAIN
<222>  (1)..(82)

<400>  7

Asp Gln Leu Val Lys Glu Thr Leu Ala Gly Val His Gly Asn Gly Asp 
1               5                   10                  15      


Thr Arg Lys Ala Ser Leu Gly Ser Gln Tyr Glu Ala Val Met Ala Val 
            20                  25                  30          


Ile Asn Gly Lys Ala Ser Ala Ser Glu Lys Ser Asp Glu Glu Leu Ala 
        35                  40                  45              


Arg Glu Val Leu Ala Gly Lys His Gly Ala Gly Glu Asp Arg Lys Arg 
    50                  55                  60                  


Ser Leu Gly Pro Arg Tyr Glu Pro Val Gln Ala Lys Val Asn Glu Leu 
65                  70                  75                  80  


Leu Lys 
        


<210>  8
<211>  125
<212>  PRT
<213>  Streptococcus suis


<220>
<221>  GLUCOSAMINIDASE DOMAIN
<222>  (1)..(125)

<400>  8

Ile Leu Lys Lys Cys Lys Glu His Asp Ile Leu Pro Ser Tyr Ala Leu 
1               5                   10                  15      


Thr Ile Leu His Tyr Glu Gly Leu Trp Gly Thr Ser Ala Val Gly Lys 
            20                  25                  30          


Ala Asp Asn Asn Trp Gly Gly Met Thr Trp Thr Gly Gln Gly Asn Arg 
        35                  40                  45              


Pro Ser Gly Val Ile Val Thr Gln Gly Leu Ala Arg Pro Ser Asn Glu 
    50                  55                  60                  


Gly Gly His Tyr Met His Tyr Ala Thr Val Asp Asp Phe Leu Thr Asp 
65                  70                  75                  80  


Trp Phe Tyr Leu Leu Arg Lys Asp Gly Ser Tyr Lys Val Ser Gly Ala 
                85                  90                  95      


Leu Thr Phe Ser Glu Ser Ile Lys Gly Met Phe Gln Val Gly Gly Ala 
            100                 105                 110         


Lys Tyr Asp Tyr Ala Ala Ala Gly Tyr Asp Ser Tyr Leu 
        115                 120                 125 


<210>  9
<211>  1359
<212>  DNA
<213>  Streptococcus suis

<400>  9
atgacaatca atcttgaaac atccattcgt tggatgagcg accgtgtcgg caaagtctct       60

tactcaatgg actatcgtaa cggtccgaat agttatgact gctctagtgc tgtatattat      120

gcgctaatgg cgggtggtgc aatttctgca ggttgggcgg ttaacactga gtatatgcat      180

gactggttga tacgtaacgg atatgttttg gtagctgaaa ataaaccatt taacgctcaa      240

agacatgacg tttgtatttt gggtaaacgt ggctattcga gcggagcagg tggtcacgtc      300

gttatctttg tggataatgt taatgtgata cattgtaact atgcacgtaa cggaatttcc      360

attgataatt ataatcaagt gcatcgtggt atgtattact atctatatcg cccagcaaat      420

caacccagca tcagcaacaa atcactggat cagcttgtta aggagacttt ggctggggta      480

catggcaacg gggacacccg taaggcaagt cttggcagtc aatacgaggc tgtcatggcg      540

gttatcaatg gcaaagcttc ggcaagcgag aaatctgatg aggaacttgc tagggaagtc      600

ttagcaggta agcacggggc tggagaggac cgaaaacggt cattaggacc acgctatgag      660

cctgttcaag ccaaggtcaa cgaattgctc aaggctaagg aaaaaccgtc tgagacggcc      720

aaaaatgaac cacagacggt gcaattcaag gaggacgggg acttgtcttt caatggtgcc      780

attcttaaga agtctgtcct cgaaattatc ctgaaaaagt gtaaagaaca tgacatctta      840

ccaagctatg ccctaactat cctacactat gaagggcttt ggggcacttc tgctgtcggt      900

aaggccgaca acaactgggg cggtatgacc tggactggcc aaggcaaccg tccgagcgga      960

gtaattgtga ctcaaggttt ggctcggcca tcgaacgagg gaggccacta catgcactat     1020

gccaccgtgg atgatttcct gacggactgg ttctacctgc ttcgcaagga cgggtcttac     1080

aaggtatctg gtgcattgac cttcagcgag tccattaagg gcatgttcca ggttggcgga     1140

gctaaatacg actatgcagc cgccggctac gatagttacc tggtcggcgc cactagcagg     1200

ctaaaagcta tcgagtccga aaatggcagt ctgacacggt ttgatgccac atcaaataat     1260

gtccattcgg ttgaccctga taaaatctct gttgatattg acggcattga agttacgatc     1320

aatggtgttg tctacaagct ggaaaagaaa ccagtctaa                            1359


<210>  10
<211>  738
<212>  DNA
<213>  Streptococcus suis

<400>  10
atgacaacag taaatgaagc attaaataat gtaagagctc aggttgggtc cggtgtgtct       60

gttggcaacg gcgaatgcta cgctttggct agttggtacg agcgcatgat tagtccggat      120

gcaactgtcg gacttggcgc tggtgtgggc tgggtcagcg gtgcaatcgg cgatacaatc      180

tctgccaaaa acatcggctc atcatacaac tggcaagcta acggctggac agtttccaca      240

tctggtccat ttaaagcagg tcagattgtg acgcttgggg caacaccagg aaacccttac      300

ggacatgtgg taatcgtcga agcagtggac ggcgatagat tgactatttt ggagcaaaac      360

tacggcggga aacgttatcc cgtccgtaat tattacagcg ctgcaagcta tcgtcaacag      420

gtcgtgcatt acatcacacc gcctggcacg gtcgcacagt cagcacccaa ccttgcaggc      480

tctcgttcct atcgcgagac gggcactatg actgtcacgg tcgatgctct caatgttcgc      540

agggcgccaa atacttcagg cgagattgta gcagtataca agcgtggtga atcatttgac      600

tatgatactg tcatcatcga tgtcaatggc tatgtctggg tgtcttacat aggcggcagc      660

ggcaaacgta actacgttgc gacgggcgct accaaagacg gtaagcgttt cggcaatgct      720

tggggtacat ttaaataa                                                    738


