                         SEQUENCE LISTING

<110>  The UAB Research Foundation
 
<120>  Isopeptide Bond Formation In Bacillus Species and Uses Thereof

<130>  U2011-0041

<150>  61/474,194
<151>  2011-04-04

<150>  13/324,864
<151>  2011-12-13

<150>  61/489,157
<151>  2011-05-23

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  400
<212>  PRT
<213>  Bacillus anthracis

<400>  1

Met Ser Asn Asn Asn Tyr Ser Asn Gly Leu Asn Pro Asp Glu Ser Leu 
1               5                   10                  15      


Ser Ala Ser Ala Phe Asp Pro Asn Leu Val Gly Pro Thr Leu Pro Pro 
            20                  25                  30          


Ile Pro Pro Phe Thr Leu Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro 
        35                  40                  45              


Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr 
    50                  55                  60                  


Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly 
65                  70                  75                  80  


Asp Thr Gly Thr Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro 
                85                  90                  95      


Thr Gly Pro Thr Gly Asp Thr Gly Thr Thr Gly Pro Thr Gly Pro Thr 
            100                 105                 110         


Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly 
        115                 120                 125             


Pro Thr Gly Pro Thr Gly Pro Thr Gly Asp Thr Gly Thr Thr Gly Pro 
    130                 135                 140                 


Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Asp Thr 
145                 150                 155                 160 


Gly Thr Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly 
                165                 170                 175     


Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro 
            180                 185                 190         


Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Asp Thr Gly Thr Thr 
        195                 200                 205             


Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly 
    210                 215                 220                 


Asp Thr Gly Thr Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Pro 
225                 230                 235                 240 


Thr Gly Pro Thr Gly Pro Thr Gly Pro Thr Gly Ala Thr Gly Leu Thr 
                245                 250                 255     


Gly Pro Thr Gly Pro Thr Gly Pro Ser Gly Leu Gly Leu Pro Ala Gly 
            260                 265                 270         


Leu Tyr Ala Phe Asn Ser Gly Gly Ile Ser Leu Asp Leu Gly Ile Asn 
        275                 280                 285             


Asp Pro Val Pro Phe Asn Thr Val Gly Ser Gln Phe Gly Thr Ala Ile 
    290                 295                 300                 


Ser Gln Leu Asp Ala Asp Thr Phe Val Ile Ser Glu Thr Gly Phe Tyr 
305                 310                 315                 320 


Lys Ile Thr Val Ile Ala Asn Thr Ala Thr Ala Ser Val Leu Gly Gly 
                325                 330                 335     


Leu Thr Ile Gln Val Asn Gly Val Pro Val Pro Gly Thr Gly Ser Ser 
            340                 345                 350         


Leu Ile Ser Leu Gly Ala Pro Ile Val Ile Gln Ala Ile Thr Gln Ile 
        355                 360                 365             


Thr Thr Thr Pro Ser Leu Val Glu Val Ile Val Thr Gly Leu Gly Leu 
    370                 375                 380                 


Ser Leu Ala Leu Gly Thr Ser Ala Ser Ile Ile Ile Glu Lys Val Ala 
385                 390                 395                 400 


<210>  2
<211>  167
<212>  PRT
<213>  Bacillus anthracis

<400>  2

Met Phe Ser Ser Asp Cys Glu Phe Thr Lys Ile Asp Cys Glu Ala Lys 
1               5                   10                  15      


Pro Ala Ser Thr Leu Pro Ala Phe Gly Phe Ala Phe Asn Ala Ser Ala 
            20                  25                  30          


Pro Gln Phe Ala Ser Leu Phe Thr Pro Leu Leu Leu Pro Ser Val Ser 
        35                  40                  45              


Pro Asn Pro Asn Ile Thr Val Pro Val Ile Asn Asp Thr Val Ser Val 
    50                  55                  60                  


Gly Asp Gly Ile Arg Ile Leu Arg Ala Gly Ile Tyr Gln Ile Ser Tyr 
65                  70                  75                  80  


Thr Leu Thr Ile Ser Leu Asp Asn Ser Pro Val Ala Pro Glu Ala Gly 
                85                  90                  95      


Arg Phe Phe Leu Ser Leu Gly Thr Pro Ala Asn Ile Ile Pro Gly Ser 
            100                 105                 110         


Gly Thr Ala Val Arg Ser Asn Val Ile Gly Thr Gly Glu Val Asp Val 
        115                 120                 125             


Ser Ser Gly Val Ile Leu Ile Asn Leu Asn Pro Gly Asp Leu Ile Arg 
    130                 135                 140                 


Ile Val Pro Val Glu Leu Ile Gly Thr Val Asp Ile Arg Ala Ala Ala 
145                 150                 155                 160 


Leu Thr Val Ala Gln Ile Ser 
                165         


<210>  3
<211>  152
<212>  PRT
<213>  Bacillus anthracis

<400>  3

Met Ser Cys Asn Glu Asn Lys His His Gly Ser Ser His Cys Val Val 
1               5                   10                  15      


Asp Val Val Lys Phe Ile Asn Glu Leu Gln Asp Cys Ser Thr Thr Thr 
            20                  25                  30          


Cys Gly Ser Gly Cys Glu Ile Pro Phe Leu Gly Ala His Asn Thr Ala 
        35                  40                  45              


Ser Val Ala Asn Thr Arg Pro Phe Ile Leu Tyr Thr Lys Ala Gly Ala 
    50                  55                  60                  


Pro Phe Glu Ala Phe Ala Pro Ser Ala Asn Leu Thr Ser Cys Arg Ser 
65                  70                  75                  80  


Pro Ile Phe Arg Val Glu Ser Val Asp Asp Asp Ser Cys Ala Val Leu 
                85                  90                  95      


Arg Val Leu Ser Val Val Leu Gly Asp Ser Ser Pro Val Pro Pro Thr 
            100                 105                 110         


Asp Asp Pro Ile Cys Thr Phe Leu Ala Val Pro Asn Ala Arg Leu Val 
        115                 120                 125             


Ser Thr Ser Thr Cys Ile Thr Val Asp Leu Ser Cys Phe Cys Ala Ile 
    130                 135                 140                 


Gln Cys Leu Arg Asp Val Thr Ile 
145                 150         


<210>  4
<211>  156
<212>  PRT
<213>  Bacillus anthracis

<400>  4

Met Ser Cys Asn Cys Asn Glu Asp His His His His Asp Cys Asp Phe 
1               5                   10                  15      


Asn Cys Val Ser Asn Val Val Arg Phe Ile His Glu Leu Gln Glu Cys 
            20                  25                  30          


Ala Thr Thr Thr Cys Gly Ser Gly Cys Glu Val Pro Phe Leu Gly Ala 
        35                  40                  45              


His Asn Ser Ala Ser Val Ala Asn Thr Arg Pro Phe Ile Leu Tyr Thr 
    50                  55                  60                  


Lys Ala Gly Ala Pro Phe Glu Ala Phe Ala Pro Ser Ala Asn Leu Thr 
65                  70                  75                  80  


Ser Cys Arg Ser Pro Ile Phe Arg Val Glu Ser Ile Asp Asp Asp Asp 
                85                  90                  95      


Cys Ala Val Leu Arg Val Leu Ser Val Val Leu Gly Asp Thr Ser Pro 
            100                 105                 110         


Val Pro Pro Thr Asp Asp Pro Ile Cys Thr Phe Leu Ala Val Pro Asn 
        115                 120                 125             


Ala Arg Leu Ile Ser Thr Asn Thr Cys Leu Thr Val Asp Leu Ser Cys 
    130                 135                 140                 


Phe Cys Ala Ile Gln Cys Leu Arg Asp Val Thr Ile 
145                 150                 155     


<210>  5
<211>  186
<212>  PRT
<213>  Bacillus anthracis

<400>  5

Met Lys Arg Asp Ile Arg Lys Ala Val Glu Glu Ile Lys Ser Ala Gly 
1               5                   10                  15      


Met Glu Asp Phe Leu His Gln Asp Pro Ser Thr Phe Asp Cys Asp Asp 
            20                  25                  30          


Asp Cys Thr Thr Lys Ile Glu Cys Ser Asp Asp Cys Asn Cys Pro Arg 
        35                  40                  45              


Thr Arg Cys Thr Arg Val Lys His Cys Thr Phe Val Thr Lys Cys Thr 
    50                  55                  60                  


His Val Lys Lys Trp Thr Phe Val Thr Lys Cys Thr Arg Val Arg Val 
65                  70                  75                  80  


Gln Lys Trp Thr Phe Val Thr Lys Val Thr Arg Arg Lys Glu Cys Val 
                85                  90                  95      


Leu Val Thr Lys Arg Thr Arg Arg Lys His Cys Thr Phe Val Thr Lys 
            100                 105                 110         


Cys Val Arg Phe Glu Lys Lys Phe Tyr Trp Thr Lys Arg Cys Tyr Cys 
        115                 120                 125             


Lys Lys Cys Glu Phe Phe Pro His Gly His Gly Gly Ser Cys Asp Asp 
    130                 135                 140                 


Ser Cys Asp His Gly Lys Asp Cys His Asp Asp Gly His Lys Trp Asn 
145                 150                 155                 160 


Asp Cys Lys Gly Gly His Lys Phe Pro Ser Cys Lys Asn Lys Lys Phe 
                165                 170                 175     


Asp His Phe Trp Tyr Lys Lys Arg Asn Cys 
            180                 185     


<210>  6
<211>  180
<212>  PRT
<213>  Bacillus anthracis

<400>  6

Met Ser Glu Phe Arg Glu Ile Ile Thr Lys Ala Val Val Gly Lys Gly 
1               5                   10                  15      


Arg Lys Tyr Thr Lys Ser Thr His Thr Cys Glu Ser Asn Asn Glu Pro 
            20                  25                  30          


Thr Ser Ile Leu Gly Cys Trp Val Ile Asn His Ser Tyr Glu Ala Arg 
        35                  40                  45              


Lys Asn Gly Lys His Val Glu Ile Glu Gly Phe Tyr Asp Val Asn Thr 
    50                  55                  60                  


Trp Tyr Ser Phe Asp Gly Asn Thr Lys Thr Glu Val Val Thr Glu Arg 
65                  70                  75                  80  


Val Asn Tyr Thr Asp Glu Val Ser Ile Gly Tyr Arg Asp Lys Asn Phe 
                85                  90                  95      


Ser Gly Asp Asp Leu Glu Ile Ile Ala Arg Val Ile Gln Pro Pro Asn 
            100                 105                 110         


Cys Leu Glu Ala Leu Val Ser Pro Asn Gly Asn Lys Ile Val Val Thr 
        115                 120                 125             


Val Glu Arg Glu Phe Val Thr Glu Val Val Gly Glu Thr Lys Ile Cys 
    130                 135                 140                 


Val Ser Val Asn Pro Glu Gly Cys Val Glu Ser Asp Glu Asp Phe Gln 
145                 150                 155                 160 


Ile Asp Asp Asp Glu Phe Glu Glu Leu Asp Pro Asn Phe Ile Val Asp 
                165                 170                 175     


Ala Glu Glu Glu 
            180 


<210>  7
<211>  19
<212>  PRT
<213>  Bacillus anthracis

<400>  7

Ala Phe Asp Pro Asn Leu Val Gly Pro Thr Leu Pro Pro Ile Pro Pro 
1               5                   10                  15      


Phe Thr Leu 
            


<210>  8
<211>  14
<212>  PRT
<213>  Bacillus anthracis

<400>  8

Ala Phe Asp Pro Asn Leu Val Gly Pro Thr Leu Pro Pro Ile 
1               5                   10                  


<210>  9
<211>  13
<212>  PRT
<213>  Bacillus anthracis

<400>  9

Phe Asp Pro Asn Leu Val Gly Pro Thr Leu Pro Pro Ile 
1               5                   10              


<210>  10
<211>  9
<212>  PRT
<213>  Bacillus anthracis

<400>  10

Ala Phe Asp Pro Asn Leu Pro Pro Ile 
1               5                   


<210>  11
<211>  8
<212>  PRT
<213>  Bacillus anthracis

<400>  11

Phe Asp Pro Asn Leu Pro Pro Ile 
1               5               


<210>  12
<211>  9
<212>  PRT
<213>  Bacillus anthracis

<400>  12

Leu Val Gly Pro Thr Leu Pro Pro Ile 
1               5                   


<210>  13
<211>  8
<212>  PRT
<213>  Bacillus anthracis

<400>  13

Val Gly Pro Thr Leu Pro Pro Ile 
1               5               


<210>  14
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial donor sequence

<400>  14

Leu Val Gly Pro Thr Leu Pro Pro Ile 
1               5                   


<210>  15
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  donor consensus sequence

<400>  15

Val Gly Pro Thr Leu Pro Pro Ile 
1               5               


<210>  16
<211>  29
<212>  PRT
<213>  Bacillus anthracis

<400>  16

Lys Arg Asp Ile Arg Lys Ala Val Glu Glu Ile Lys Ser Ala Gly Met 
1               5                   10                  15      


Glu Asp Phe Leu His Gln Asp Pro Ser Thr Phe Asp Cys 
            20                  25                  


<210>  17
<211>  20
<212>  PRT
<213>  Bacillus anthracis

<400>  17

Val Glu Glu Ile Lys Ser Ala Gly Met Glu Asp Phe Leu His Gln Asp 
1               5                   10                  15      


Pro Ser Thr Phe 
            20  


<210>  18
<211>  11
<212>  PRT
<213>  Bacillus anthracis

<400>  18

Lys Ser Ala Gly Met Glu Asp Phe Leu His Gln 
1               5                   10      


<210>  19
<211>  19
<212>  PRT
<213>  Bacillus anthracis

<400>  19

Ser Cys Asn Glu Asn Lys His His Gly Ser Ser His Cys Val Val Asp 
1               5                   10                  15      


Val Val Lys 
            


<210>  20
<211>  6
<212>  PRT
<213>  Bacillus anthracis

<400>  20

Ser Cys Asn Glu Asn Lys 
1               5       


<210>  21
<211>  11
<212>  PRT
<213>  Bacillus anthracis

<400>  21

Ser Cys Asn Glu Asn Lys His His Gly Ser Ser 
1               5                   10      


<210>  22
<211>  15
<212>  PRT
<213>  Bacillus anthracis

<400>  22

Cys Asn Glu Asn Lys His His Gly Ser Ser His Cys Val Val Asp 
1               5                   10                  15  


<210>  23
<211>  19
<212>  PRT
<213>  Bacillus anthracis

<400>  23

Ser Cys Asn Cys Asn Glu Asp His His His His Asp Cys Asp Phe Asn 
1               5                   10                  15      


Cys Val Ser 
            


<210>  24
<211>  6
<212>  PRT
<213>  Bacillus anthracis

<400>  24

Ser Cys Asn Cys Asn Glu 
1               5       


<210>  25
<211>  11
<212>  PRT
<213>  Bacillus anthracis

<400>  25

Ser Cys Asn Cys Asn Glu Asp His His His His 
1               5                   10      


<210>  26
<211>  16
<212>  PRT
<213>  Bacillus anthracis

<400>  26

Ser Cys Asn Cys Asn Glu Asp His His His His Asp Cys Asp Phe Asn 
1               5                   10                  15      


