                         SEQUENCE LISTING

<110>  University of Durham
       The Food and Environment Research Agency (FERA), 
       representing the Secretary of State for Environment, Food and 
       Rural Affairs
 
<120>  Spider Toxin Pesticide

<130>  PL401462WO

<150>  GB1105418.6
<151>  2011-03-31

<160>  28    

<170>  PatentIn version 3.3

<210>  1
<211>  37
<212>  PRT
<213>  Hadronyche versuta

<400>  1

Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  2
<211>  37
<212>  PRT
<213>  Artificial

<220>
<223>  modified natrual toxin sequence

<400>  2

Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Gln Arg Cys Asp 
        35          


<210>  3
<211>  105
<212>  PRT
<213>  Galanthus nivalis

<400>  3

Asp Asn Ile Leu Tyr Ser Gly Glu Thr Leu Ser Thr Gly Glu Phe Leu 
1               5                   10                  15      


Asn Tyr Gly Ser Phe Val Phe Ile Met Gln Glu Asp Cys Asn Leu Val 
            20                  25                  30          


Leu Tyr Asp Val Asp Lys Pro Ile Trp Ala Thr Asn Thr Gly Gly Leu 
        35                  40                  45              


Ser Arg Ser Cys Phe Leu Ser Met Gln Thr Asp Gly Asn Leu Val Val 
    50                  55                  60                  


Tyr Asn Pro Ser Asn Lys Pro Ile Trp Ala Ser Asn Thr Gly Gly Gln 
65                  70                  75                  80  


Asn Gly Asn Tyr Val Cys Ile Leu Gln Lys Asp Arg Asn Val Val Ile 
                85                  90                  95      


Tyr Gly Thr Asp Arg Trp Ala Thr Gly 
            100                 105 


<210>  4
<211>  153
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein

<400>  4

Asp Asn Ile Leu Tyr Ser Gly Glu Thr Leu Ser Thr Gly Glu Phe Leu 
1               5                   10                  15      


Asn Tyr Gly Ser Phe Val Phe Ile Met Gln Glu Asp Cys Asn Leu Val 
            20                  25                  30          


Leu Tyr Asp Val Asp Lys Pro Ile Trp Ala Thr Asn Thr Gly Gly Leu 
        35                  40                  45              


Ser Arg Ser Cys Phe Leu Ser Met Gln Thr Asp Gly Asn Leu Val Val 
    50                  55                  60                  


Tyr Asn Pro Ser Asn Lys Pro Ile Trp Ala Ser Asn Thr Gly Gly Gln 
65                  70                  75                  80  


Asn Gly Asn Tyr Val Cys Ile Leu Gln Lys Asp Arg Asn Val Val Ile 
                85                  90                  95      


Tyr Gly Thr Asp Arg Trp Ala Thr Gly Ala Ala Ala Ser Pro Thr Cys 
            100                 105                 110         


Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn Cys Cys Ser Gln 
        115                 120                 125             


Ser Cys Thr Phe Lys Glu Asn Glu Asn Gly Asn Thr Val Lys Arg Cys 
    130                 135                 140                 


Asp Val Asp His His His His His His 
145                 150             


<210>  5
<211>  151
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein

<400>  5

His His His His His His Asp Asn Ile Leu Tyr Ser Gly Glu Thr Leu 
1               5                   10                  15      


Ser Thr Gly Glu Phe Leu Asn Tyr Gly Ser Phe Val Phe Ile Met Gln 
            20                  25                  30          


Glu Asp Cys Asn Leu Val Leu Tyr Asp Val Asp Lys Pro Ile Trp Ala 
        35                  40                  45              


Thr Asn Thr Gly Gly Leu Ser Arg Ser Cys Phe Leu Ser Met Gln Thr 
    50                  55                  60                  


Asp Gly Asn Leu Val Val Tyr Asn Pro Ser Asn Lys Pro Ile Trp Ala 
65                  70                  75                  80  


Ser Asn Thr Gly Gly Gln Asn Gly Asn Tyr Val Cys Ile Leu Gln Lys 
                85                  90                  95      


Asp Arg Asn Val Val Ile Tyr Gly Thr Asp Arg Trp Ala Thr Gly Ala 
            100                 105                 110         


Ala Ala Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn 
        115                 120                 125             


Glu Asn Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Asn Gly 
    130                 135                 140                 


Asn Thr Val Gln Arg Cys Asp 
145                 150     


<210>  6
<211>  146
<212>  PRT
<213>  Artificial

<220>
<223>  fusion protein

<400>  6

Ala Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu 
1               5                   10                  15      


Asn Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Asn Gly Asn 
            20                  25                  30          


Thr Val Lys Arg Cys Asp Ala Ala Ala Asp Asn Ile Leu Tyr Ser Gly 
        35                  40                  45              


Glu Thr Leu Ser Thr Gly Glu Phe Leu Asn Tyr Gly Ser Phe Val Phe 
    50                  55                  60                  


Ile Met Gln Glu Asp Cys Asn Leu Val Leu Tyr Asp Val Asp Lys Pro 
65                  70                  75                  80  


Ile Trp Ala Thr Asn Thr Gly Gly Leu Ser Arg Ser Cys Phe Leu Ser 
                85                  90                  95      


Met Gln Thr Asp Gly Asn Leu Val Val Tyr Asn Pro Ser Asn Lys Pro 
            100                 105                 110         


Ile Trp Ala Ser Asn Thr Gly Gly Gln Asn Gly Asn Tyr Val Cys Ile 
        115                 120                 125             


Leu Gln Lys Asp Arg Asn Val Val Ile Tyr Gly Thr Asp Arg Trp Ala 
    130                 135                 140                 


Thr Gly 
145     


<210>  7
<211>  154
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein

<400>  7

Ala Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu 
1               5                   10                  15      


Asn Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Asn Gly Asn 
            20                  25                  30          


Thr Val Lys Arg Cys Asp Ala Ala Ala Asp Asn Ile Leu Tyr Ser Gly 
        35                  40                  45              


Glu Thr Leu Ser Thr Gly Glu Phe Leu Asn Tyr Gly Ser Phe Val Phe 
    50                  55                  60                  


Ile Met Gln Glu Asp Cys Asn Leu Val Leu Tyr Asp Val Asp Lys Pro 
65                  70                  75                  80  


Ile Trp Ala Thr Asn Thr Gly Gly Leu Ser Arg Ser Cys Phe Leu Ser 
                85                  90                  95      


Met Gln Thr Asp Gly Asn Leu Val Val Tyr Asn Pro Ser Asn Lys Pro 
            100                 105                 110         


Ile Trp Ala Ser Asn Thr Gly Gly Gln Asn Gly Asn Tyr Val Cys Ile 
        115                 120                 125             


Leu Gln Lys Asp Arg Asn Val Val Ile Tyr Gly Thr Asp Arg Trp Ala 
    130                 135                 140                 


Thr Gly Val Asp His His His His His His 
145                 150                 


<210>  8
<211>  146
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein

<400>  8

Ala Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu 
1               5                   10                  15      


Asn Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Asn Gly Asn 
            20                  25                  30          


Thr Val Gln Arg Cys Asp Ala Ala Ala Asp Asn Ile Leu Tyr Ser Gly 
        35                  40                  45              


Glu Thr Leu Ser Thr Gly Glu Phe Leu Asn Tyr Gly Ser Phe Val Phe 
    50                  55                  60                  


Ile Met Gln Glu Asp Cys Asn Leu Val Leu Tyr Asp Val Asp Lys Pro 
65                  70                  75                  80  


Ile Trp Ala Thr Asn Thr Gly Gly Leu Ser Arg Ser Cys Phe Leu Ser 
                85                  90                  95      


Met Gln Thr Asp Gly Asn Leu Val Val Tyr Asn Pro Ser Asn Lys Pro 
            100                 105                 110         


Ile Trp Ala Ser Asn Thr Gly Gly Gln Asn Gly Asn Tyr Val Cys Ile 
        115                 120                 125             


Leu Gln Lys Asp Arg Asn Val Val Ile Tyr Gly Thr Asp Arg Trp Ala 
    130                 135                 140                 


Thr Gly 
145     


<210>  9
<211>  152
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein

<400>  9

Ala Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu 
1               5                   10                  15      


Asn Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Asn Gly Asn 
            20                  25                  30          


Thr Val Gln Arg Cys Asp Ala Ala Ala Asp Asn Ile Leu Tyr Ser Gly 
        35                  40                  45              


Glu Thr Leu Ser Thr Gly Glu Phe Leu Asn Tyr Gly Ser Phe Val Phe 
    50                  55                  60                  


Ile Met Gln Glu Asp Cys Asn Leu Val Leu Tyr Asp Val Asp Lys Pro 
65                  70                  75                  80  


Ile Trp Ala Thr Asn Thr Gly Gly Leu Ser Arg Ser Cys Phe Leu Ser 
                85                  90                  95      


Met Gln Thr Asp Gly Asn Leu Val Val Tyr Asn Pro Ser Asn Lys Pro 
            100                 105                 110         


Ile Trp Ala Ser Asn Thr Gly Gly Gln Asn Gly Asn Tyr Val Cys Ile 
        115                 120                 125             


Leu Gln Lys Asp Arg Asn Val Val Ile Tyr Gly Thr Asp Arg Trp Ala 
    130                 135                 140                 


Thr Gly His His His His His His 
145                 150         


<210>  10
<211>  246
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein

<400>  10

Met Arg Phe Pro Ser Phe Leu Leu Leu Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Glu Lys Arg Glu Ala Glu Ala Ala Ala Ser Pro Thr Cys Ile 
                85                  90                  95      


Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn Cys Cys Ser Gln Ser 
            100                 105                 110         


Cys Thr Phe Lys Glu Asn Glu Asn Gly Asn Thr Val Gln Arg Cys Asp 
        115                 120                 125             


Ala Ala Ala Asp Asn Ile Leu Tyr Ser Gly Glu Thr Leu Ser Thr Gly 
    130                 135                 140                 


Glu Phe Leu Asn Tyr Gly Ser Phe Val Phe Ile Met Gln Glu Asp Cys 
145                 150                 155                 160 


Asn Leu Val Leu Tyr Asp Val Asp Lys Pro Ile Trp Ala Thr Asn Thr 
                165                 170                 175     


Gly Gly Leu Ser Arg Ser Cys Phe Leu Ser Met Gln Thr Asp Gly Asn 
            180                 185                 190         


Leu Val Val Tyr Asn Pro Ser Asn Lys Pro Ile Trp Ala Ser Asn Thr 
        195                 200                 205             


Gly Gly Gln Asn Gly Asn Tyr Val Cys Ile Leu Gln Lys Asp Arg Asn 
    210                 215                 220                 


Val Val Ile Tyr Gly Thr Asp Arg Trp Ala Thr Gly Val Asp His His 
225                 230                 235                 240 


His His His His Ser Arg 
                245     


<210>  11
<211>  111
<212>  DNA
<213>  Artificial

<220>
<223>  nucleic acid encoding modified toxin

<400>  11
tctccaactt gtattccatc tggtcaacca tgtccatata atgaaaattg ttgttctcaa     60

tcttgtactt ttaaagaaaa tgaaaatggt aatactgttc aaagatgtga t             111


<210>  12
<211>  314
<212>  DNA
<213>  Galanthus nivalis

<400>  12
gacaatattt tgtactccgg tgagactctc tctacagggg aatttctcaa ctacggaagt     60

ttcgttttta tcatgcaaga ggactgcaat ctggtcttgt acgacgtgga caagccaatc    120

tgggcaacaa acacaggtgg tctctcccgt agctgcttcc tcagcatgca gactgatggg    180

aacctcgtgg tgtacaaccc atcgaacaaa ccgatttggg caagcaacac tggaggccaa    240

aatgggaatt acgtgtgcat cctacagaag gataggaatg ttgtgatcta cggaactgat    300

cgttgggcca ctgg                                                      314


<210>  13
<211>  735
<212>  DNA
<213>  Artificial

<220>
<223>  nucleic acid for signal peptide fused to fusion protein

<400>  13
atgagatttc cttcattttt actgctggtt ttattcgcag catcctccgc attagctgct     60

ccagtcaaca ctacaacaga agatgaaacg gcacaaattc cggctgaagc tgtcatcggt    120

tactcagatt tagaagggga tttcgatgtt gctgttttgc cattttccaa cagcacaaat    180

aacgggttat tgtttataaa tactactatt gccagcattg ctgctaaaga agaaggggta    240

tctctcgaga aaagagaggc tgaagctgca gcatctccaa cttgtattcc atctggtcaa    300

ccatgtccat ataatgaaaa ttgttgttct caatcttgta cttttaaaga aaatgaaaat    360

ggtaatactg ttcaaagatg tgatgcggcc gccgacaata ttttgtactc cggtgagact    420

ctctctacag gggaatttct caactacgga agtttcgttt ttatcatgca agaggactgc    480

aatctggtct tgtacgacgt ggacaagcca atctgggcaa caaacacagg tggtctctcc    540

cgtagctgct tcctcagcat gcagactgat gggaacctcg tggtgtacaa cccatcgaac    600

aaaccgattt gggcaagcaa cactggaggc caaaatggga attacgtgtg catcctacag    660

aaggatagga atgttgtgat ctacggaact gatcgttggg ccactggagt ggaccatcat    720

catcatcatc attga                                                     735


<210>  14
<211>  37
<212>  PRT
<213>  Mesobuthus tamulus

<400>  14

Arg Cys Gly Pro Cys Phe Thr Thr Asp Pro Gln Thr Gln Ala Lys Cys 
1               5                   10                  15      


Ser Glu Cys Cys Gly Arg Lys Gly Gly Val Cys Lys Gly Pro Gln Cys 
            20                  25                  30          


Ile Cys Gly Ile Gln 
        35          


<210>  15
<211>  23
<212>  PRT
<213>  Artificial

<220>
<223>  GNA signal peptide

<400>  15

Met Ala Lys Ala Ser Leu Leu Ile Leu Ala Ala Ile Phe Leu Gly Val 
1               5                   10                  15      


Ile Thr Pro Ser Cys Leu Ser 
            20              


<210>  16
<211>  171
<212>  PRT
<213>  Artificial

<220>
<223>  Fusion protein

<400>  16

Met Ala Lys Ala Ser Leu Leu Ile Leu Ala Ala Ile Phe Leu Gly Val 
1               5                   10                  15      


Ile Thr Pro Ser Cys Leu Ser Ala Ala Ala Arg Cys Gly Pro Cys Phe 
            20                  25                  30          


Thr Thr Asp Pro Gln Thr Gln Ala Lys Cys Ser Glu Cys Cys Gly Arg 
        35                  40                  45              


Lys Gly Gly Val Cys Lys Gly Pro Gln Cys Ile Cys Gly Ile Gln Ala 
    50                  55                  60                  


Ala Ala Asp Asn Ile Leu Tyr Ser Gly Glu Thr Leu Ser Thr Gly Glu 
65                  70                  75                  80  


Phe Leu Asn Tyr Gly Ser Phe Val Phe Ile Met Gln Glu Asp Cys Asn 
                85                  90                  95      


Leu Val Leu Tyr Asp Val Asp Lys Pro Ile Trp Ala Thr Asn Thr Gly 
            100                 105                 110         


Gly Leu Ser Arg Ser Cys Phe Leu Ser Met Gln Thr Asp Gly Asn Leu 
        115                 120                 125             


Val Val Tyr Asn Pro Ser Asn Lys Pro Ile Trp Ala Ser Asn Thr Gly 
    130                 135                 140                 


Gly Gln Asn Gly Asn Tyr Val Cys Ile Leu Gln Lys Asp Arg Asn Val 
145                 150                 155                 160 


Val Ile Tyr Gly Thr Asp Arg Trp Ala Thr Gly 
                165                 170     


<210>  17
<211>  516
<212>  DNA
<213>  Artificial

<220>
<223>  nucleic acid for fusion protein

<400>  17
atggctaagg caagtctcct cattttggcc gccatcttcc ttggtgtcat cacaccatct     60

tgcctgagtg ctgcagcaag gtgtggtcct tgctttacaa ctgatcctca aacacaagcc    120

aagtgtagtg agtgttgtgg gcgaaagggt ggagtatgca agggcccaca atgtatctgt    180

ggtatacaag cggccgccga caatattttg tactccggtg agactctctc tacaggggaa    240

tttctcaact acggaagttt cgtttttatc atgcaagagg actgcaatct ggtcttgtac    300

gacgtggaca agccaatctg ggcaacaaac acaggtggtc tctcccgtag ctgcttcctc    360

agcatgcaga ctgatgggaa cctcgtggtg tacaacccat cgaacaaacc gatttgggca    420

agcaacactg gaggccaaaa tgggaattac gtgtgcatcc tacagaagga taggaatgtt    480

gtgatctacg gaactgatcg ttgggctact ggatga                              516


<210>  18
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide sequence

<400>  18
tggtctctcc cgtagctgct t                                               21


<210>  19
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide sequence

<400>  19
atcgaacaaa ccgatttggg                                                 20


<210>  20
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide sequence

<400>  20
cggtatgtgt aaggccggat a                                               21


<210>  21
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide sequence

<400>  21
acgaccgatg ggaacactgt                                                 20


<210>  22
<211>  57
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide sequence

<400>  22
gcatctccaa cttgtattcc atctggtcaa ccatgtccat ataatgaaaa ttgttgt        57


<210>  23
<211>  59
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide sequence

<400>  23
tctcaatctt gtacttttaa agaaaatgaa aatggtaata ctgttaaaag atgtgatgc      59


<210>  24
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucletide sequence

<400>  24
acgtcgtaga ggttgaacat aaggtagacc agttggtaca                           40


<210>  25
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide sequence

<400>  25
ggtatattac ttttaacaac aagagttaga acatgaaaat tt                        42


<210>  26
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide sequence

<400>  26
cttttacttt taccattatg acaattttct acactacgcc gg                        42


<210>  27
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide sequence

<400>  27
taactgcagc atctccaact tgtattcc                                        28


<210>  28
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide sequence

<400>  28
ttagcggccg catcacatct tttaacag                                        28


